LOC104929390 cDNA ORF clone, Larimichthys crocea(large yellow croaker)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC104929390
Entrez Gene ID	104929390
Full Name	PDZ domain-containing protein 8
Synonyms	pdzd8
Gene Type	protein-coding
Organism	Larimichthys crocea(large yellow croaker)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010743901.3	XP_010742203.2	PDZ domain-containing protein 8
XM_010743901.2	XP_010742203.2	PDZ domain-containing protein 8

Pathways from BioSystems
Gene Ontology

The following LOC104929390 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC104929390 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OLa111566	XM_010743901.3 Latest version!	Larimichthys crocea PDZ domain containing 8 (pdzd8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
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OLa111566	XM_010743901.2	Larimichthys crocea PDZ domain-containing protein 8 (LOC104929390), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC104929390 ( XM_010743901.3 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010742203.2 Larimichthys crocea PDZ domain containing 8 (pdzd8), mRNA.

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CloneID	OLa111566
Clone ID Related Accession (Same CDS sequence)	XM_010743901.3 , XM_010743901.2
Accession Version	XM_010743901.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	PDZ domain-containing protein 8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040011.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_010743901.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTACT TAATATTAAT CTCCGCGTTC TCCGGAGCAG TCGTTACGTT GCTATTACAG 
TTCTTGCTGA TCTACCGGAG AAACATCGAG CCAATAGGCA GGACAGTACA GTATGTCAAA 
GTAGTGCCTG ATAACGCACT GAAGGATTAC TTTAATACCC AACATGCCGA ACCAGGCCAG 
CACCAGCAGG ACAGCACGGC ATCCACCGCA TCCAAGCAGC CAGAGGCTGG CTCGGCCCGG 
CAGCAGGAAG CGGCTGTCAG CGGCGGCAGC CCCAAACAGC CGCCGCCTCC TCACAGCGAG 
CCCGCCGATG CGGCCAAGCC CGAGACGTGC AATTTCCTAA ACGCGATCTT TTTGTTCCTG 
TTCAGGGAGC TCCGGGACAC CCCTGTGGTC AGACACTGGC TCACCAAGAA GATCAAGGTG 
GAGTTTGAGG AGCTGCTGCA GACCAAGACA GCCGGACGGC TCCTGGAGGG ACTCAGTCTC 
AGGGATATTT CCCTGGGCAA TTCTCTGCCA GTCTTTAAGA CGGCCAAACT TATGAAGCCG 
GTGCACCTGA ACGAGGACGG CATGCCCGAG GAGCTCAACT TCGAGGTGGA CATCGAATAC 
AATGGCGGCT TCCATCTGGC CATCGACGTC GAGCTGGTGT TCGGGAAGTC TGCCTACCTG 
TTCGTTAAAA TGAGGCGCGT GGTGGGTAGG CTGAGGCTGC AGTTCACGCG CATGCCCTTC 
TCCCATTGGT CATTCTCCTT CCTCGAGGAC CCGCTGGTGG ATTTTGAGGC GAAGTCCCAG 
TTTGAAGGAA GGCCGCTGCC TCAGCTCACC TCCATCATAG TCAACCAGCT GAAGCGCGTC 
ATCAAAAAGA AACACACCTT ACCAAACTAC AAAATCAGAT ACAAGCCCTT CTTCCCGTTC 
CAAGTCCAGG CTCCGCTCGG TTCGGCTTGT GATTTGGACC TGTCAGTCCA GGACAGCAGG 
CTGGTGGAAG GCCGACTCAG AGTTACCCTC ATTGAATGTA GCAGACTGTT CATCCTGGGC 
TCCTATGACA GAGAAACCTA TGTTCACTGC ACCTTGGAGC TGAGCAGCCA CCAGTGGAAG 
GAGAAGACTC GCAGCTCCAT CAAACAGACG GAGGTTATCA AGGGGCCATG TGGCAGTGTG 
GGGATGACGT TCAGACATGT CCCTGCCAGT GAGGGGGATG CAGTGCATGT CAGCATCGAG 
ACAGTCATGC CCAACTCGCC TGCAGCCTTG GCAGACTTGC AAAGGGGTGA TCGCCTAATC 
GCCATTGGAG GTGTCAAAGT GACATCCTCA GTTCAAGTGC CCAAACTACT GAAGCAGGCT 
GGAGACAGGG TGGTGGTACT TTATGAGAGA CCTGTTCGTC ACCAGCCTCC CTCTTTTGGA 
AGCCTGGGAA CTCTTCAAGA AGGGTTTGGG CAGCTTGAGG AAACAGGTTT TATTTCCCAG 
CCAGGGGGTT ATGAAGAAGA CCCAGCCCCT ATCACCACCC TTTCTGACAT ATCCGACAGC 
AAAGACATTG ACTCTGAGTT TGAAGAGTTG ATTGTGGACT CTAGTGTGAA ACCTAGCCAG 
ACAGGTGGCC CTAACAGCAG TATCACAAAT ACCAGTGACA CTAAGGATGA TTCCTTACTT 
ACAGTAAACC AAAGCCCCAA AAGAACTGTG GCCAACTTGG CCTCTAAACC CCTTGGTACC 
ATATCACCTA TACTCAACCG CAAGTTAAAC CTGGTGGGTC TCCAGTCACC ACTAAAGCCC 
CAGCCCAAAG AATCACCGAA GCCCTCACCA CATAAGACAA GTAGTGATCC AGGGGAGGGT 
GTGCAACGCC CTACTGTCCC TCCCCCACCT CCTCCTTCTC GACCCCCAGT GCCGCCAAGA 
CCTCAGATAA GGTTGACATC AGCCTCTTCA GAAACCAGTC TTTTGGAAGG CGTTGATTCT 
GTTACAACTA CAAATGCTAC TGTTGCTCCT ACCGTAACTA CCAGCAGCTC TGAAAAATCT 
CCAGAAAAAG TTCCTCTCGC TGGAGCCAGT GAGGACAAGG CTGGAACTGA GAAGATATGT 
GTCAAACAGG CTGAGGTGAA ACAGATTACC AGGCCAGCAG AGTCCAGTGA TGAGCTGCCA 
GTCCCTTCCA CTGTGACCAG CAGCAGCAAG GCCGACCAAG CAAAGGACAA AACATCAGAG 
AGTTCCTGCA GCACACGGGA TATTCTAGAG GACCACTCTC TCTGGGAATC CCCAGAAACC 
ATGTTTCGTC ACCAGACAGC ACGCTGGCCC AAGGCCTCTA TGGTGTTTGA GGTGGAGAGC 
AACCATCAGT ATCTTAATGT CGCCCTGTGG TGCAAAGATC CCTTTAAGCT TGGCAGTTTG 
TTGTGCCTGG GTCATGCCAG CCTCCAGCTG GAGCATTTAG CCATGGAATG TATGGCCACA 
TCTTCTGCAG AGTACCAGAG CACCTTTAGG TTGGGGCCTC CAGAACCCAG AGCTAACGTC 
AGCCGTACTG CACTACGTAG CCTGAGCACC CATAAGGGCT TCAATGAGAA GCTGTGTTAC 
GGAGATGTTA CTCTGAACTT CTGTTACTTA GCTGAGGGTG AATTAGAGTA TCCAGGAGGG 
CTGGCAGAAC GGGAGCGAGA GGGAAGTCTC CATGATGAAG ATCTAAGAGA GAGAGAGCGG 
GAGCATATCC CTTCAGCACC ACGTGATGAC TTGGCCTACA GCACCATGCA GTTGGTGGAG 
GTGCGTCACA ACTTCCAGGA CACCCAGTTC CAGAATCCCA CCTGGTGTGA ATACTGCAAG 
AAGAAGGTTT GGACCAAGGC TGCCTCTCAG TGTATGGTCT GCACCTATGT TTGCCACAAA 
AAGTGCCAGG ACAAGTGCCT CACTGAGAAT CCTTTCTGTG TGGCAGCAAC TGAGCGTCGT 
GGAGCTGACC CTGAAGCCAA ATCCACCATA AACCGGGCCA CCACTGGCTT AACTCGGCAT 
ATCATCAACA CCAGCTCCCG TTTGCTTAAT CTCCGCCAGG TGCCCAAGAC TCGGCTGGTT 
GAGCAGGTGG CTGATGTGGT GCCTGGAGTG GTAGAGCCCT CACCCAAGCA TACACCCAAC 
ACCTCAGACA ATGAGAGCAG TGATACTGAG ACCTACACCA GTGGCGCCAG CCCCTCAAAG 
CAGCCCTCTG GCAGCGGTGG CAGCAGTAAA CTTGTACGCA AGGAAGGTGG GTTGGATGAC 
AGTGTGTTCA TTGCTGTGAA GGAGATAGGT CGTGACCTGT ACCGGGGACT GCCCACAGAT 
GAACGCTCTC AGAAGCTTGA GTTGATGCTG GACAAATTGC AGCAGGAAAT TGACCAGGAG 
CTGGAGCACA ATAATGCTCT TCTGTTGGAG GAGAGGGAGG CCACAGATGC CCGACGCAAG 
GCCCTAATTA GTACAGCCCT GGCCAAGTCT GGGGAGAGAC TCCAGGCCCT CACCCTGCTA 
ATGATCCACT ACCGAGCAGG CATTGAGGAC CTGGAGTCAG TGGAGAGCAC CTCTCCATCA 
GAGCAGCAAG GCTTTAAGGG CAAAGGAGAA ACCCTGGAGG AAGAGGCTCT GATGGGGACA 
GAGGTCTATG ACAGTGACAT ATGCAGCCCT GTGGAGGTGC AGCTGCTGGA TGACATTACT 
GAGGAGCAAA TCTGTGTTGA GGCACTCCAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010742203.2
CDS	863..4495
Translation	MIYLILISAFSGAVVTLLLQFLLIYRRNIEPIGRTVQYVKVVPDNALKDYFNTQHAEPGQHQQDSTASTASKQPEAGSARQQEAAVSGGSPKQPPPPHSEPADAAKPETCNFLNAIFLFLFRELRDTPVVRHWLTKKIKVEFEELLQTKTAGRLLEGLSLRDISLGNSLPVFKTAKLMKPVHLNEDGMPEELNFEVDIEYNGGFHLAIDVELVFGKSAYLFVKMRRVVGRLRLQFTRMPFSHWSFSFLEDPLVDFEAKSQFEGRPLPQLTSIIVNQLKRVIKKKHTLPNYKIRYKPFFPFQVQAPLGSACDLDLSVQDSRLVEGRLRVTLIECSRLFILGSYDRETYVHCTLELSSHQWKEKTRSSIKQTEVIKGPCGSVGMTFRHVPASEGDAVHVSIETVMPNSPAALADLQRGDRLIAIGGVKVTSSVQVPKLLKQAGDRVVVLYERPVRHQPPSFGSLGTLQEGFGQLEETGFISQPGGYEEDPAPITTLSDISDSKDIDSEFEELIVDSSVKPSQTGGPNSSITNTSDTKDDSLLTVNQSPKRTVANLASKPLGTISPILNRKLNLVGLQSPLKPQPKESPKPSPHKTSSDPGEGVQRPTVPPPPPPSRPPVPPRPQIRLTSASSETSLLEGVDSVTTTNATVAPTVTTSSSEKSPEKVPLAGASEDKAGTEKICVKQAEVKQITRPAESSDELPVPSTVTSSSKADQAKDKTSESSCSTRDILEDHSLWESPETMFRHQTARWPKASMVFEVESNHQYLNVALWCKDPFKLGSLLCLGHASLQLEHLAMECMATSSAEYQSTFRLGPPEPRANVSRTALRSLSTHKGFNEKLCYGDVTLNFCYLAEGELEYPGGLAEREREGSLHDEDLREREREHIPSAPRDDLAYSTMQLVEVRHNFQDTQFQNPTWCEYCKKKVWTKAASQCMVCTYVCHKKCQDKCLTENPFCVAATERRGADPEAKSTINRATTGLTRHIINTSSRLLNLRQVPKTRLVEQVADVVPGVVEPSPKHTPNTSDNESSDTETYTSGASPSKQPSGSGGSSKLVRKEGGLDDSVFIAVKEIGRDLYRGLPTDERSQKLELMLDKLQQEIDQELEHNNALLLEEREATDARRKALISTALAKSGERLQALTLLMIHYRAGIEDLESVESTSPSEQQGFKGKGETLEEEALMGTEVYDSDICSPVEVQLLDDITEEQICVEALH

Target ORF information:

RefSeq Version	XM_010743901.3
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea PDZ domain containing 8 (pdzd8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010743901.3

ORF Insert Sequence:

ATGATTTACT TAATATTAAT CTCCGCGTTC TCCGGAGCAG TCGTTACGTT GCTATTACAG 
TTCTTGCTGA TCTACCGGAG AAACATCGAG CCAATAGGCA GGACAGTACA GTATGTCAAA 
GTAGTGCCTG ATAACGCACT GAAGGATTAC TTTAATACCC AACATGCCGA ACCAGGCCAG 
CACCAGCAGG ACAGCACGGC ATCCACCGCA TCCAAGCAGC CAGAGGCTGG CTCGGCCCGG 
CAGCAGGAAG CGGCTGTCAG CGGCGGCAGC CCCAAACAGC CGCCGCCTCC TCACAGCGAG 
CCCGCCGATG CGGCCAAGCC CGAGACGTGC AATTTCCTAA ACGCGATCTT TTTGTTCCTG 
TTCAGGGAGC TCCGGGACAC CCCTGTGGTC AGACACTGGC TCACCAAGAA GATCAAGGTG 
GAGTTTGAGG AGCTGCTGCA GACCAAGACA GCCGGACGGC TCCTGGAGGG ACTCAGTCTC 
AGGGATATTT CCCTGGGCAA TTCTCTGCCA GTCTTTAAGA CGGCCAAACT TATGAAGCCG 
GTGCACCTGA ACGAGGACGG CATGCCCGAG GAGCTCAACT TCGAGGTGGA CATCGAATAC 
AATGGCGGCT TCCATCTGGC CATCGACGTC GAGCTGGTGT TCGGGAAGTC TGCCTACCTG 
TTCGTTAAAA TGAGGCGCGT GGTGGGTAGG CTGAGGCTGC AGTTCACGCG CATGCCCTTC 
TCCCATTGGT CATTCTCCTT CCTCGAGGAC CCGCTGGTGG ATTTTGAGGC GAAGTCCCAG 
TTTGAAGGAA GGCCGCTGCC TCAGCTCACC TCCATCATAG TCAACCAGCT GAAGCGCGTC 
ATCAAAAAGA AACACACCTT ACCAAACTAC AAAATCAGAT ACAAGCCCTT CTTCCCGTTC 
CAAGTCCAGG CTCCGCTCGG TTCGGCTTGT GATTTGGACC TGTCAGTCCA GGACAGCAGG 
CTGGTGGAAG GCCGACTCAG AGTTACCCTC ATTGAATGTA GCAGACTGTT CATCCTGGGC 
TCCTATGACA GAGAAACCTA TGTTCACTGC ACCTTGGAGC TGAGCAGCCA CCAGTGGAAG 
GAGAAGACTC GCAGCTCCAT CAAACAGACG GAGGTTATCA AGGGGCCATG TGGCAGTGTG 
GGGATGACGT TCAGACATGT CCCTGCCAGT GAGGGGGATG CAGTGCATGT CAGCATCGAG 
ACAGTCATGC CCAACTCGCC TGCAGCCTTG GCAGACTTGC AAAGGGGTGA TCGCCTAATC 
GCCATTGGAG GTGTCAAAGT GACATCCTCA GTTCAAGTGC CCAAACTACT GAAGCAGGCT 
GGAGACAGGG TGGTGGTACT TTATGAGAGA CCTGTTCGTC ACCAGCCTCC CTCTTTTGGA 
AGCCTGGGAA CTCTTCAAGA AGGGTTTGGG CAGCTTGAGG AAACAGGTTT TATTTCCCAG 
CCAGGGGGTT ATGAAGAAGA CCCAGCCCCT ATCACCACCC TTTCTGACAT ATCCGACAGC 
AAAGACATTG ACTCTGAGTT TGAAGAGTTG ATTGTGGACT CTAGTGTGAA ACCTAGCCAG 
ACAGGTGGCC CTAACAGCAG TATCACAAAT ACCAGTGACA CTAAGGATGA TTCCTTACTT 
ACAGTAAACC AAAGCCCCAA AAGAACTGTG GCCAACTTGG CCTCTAAACC CCTTGGTACC 
ATATCACCTA TACTCAACCG CAAGTTAAAC CTGGTGGGTC TCCAGTCACC ACTAAAGCCC 
CAGCCCAAAG AATCACCGAA GCCCTCACCA CATAAGACAA GTAGTGATCC AGGGGAGGGT 
GTGCAACGCC CTACTGTCCC TCCCCCACCT CCTCCTTCTC GACCCCCAGT GCCGCCAAGA 
CCTCAGATAA GGTTGACATC AGCCTCTTCA GAAACCAGTC TTTTGGAAGG CGTTGATTCT 
GTTACAACTA CAAATGCTAC TGTTGCTCCT ACCGTAACTA CCAGCAGCTC TGAAAAATCT 
CCAGAAAAAG TTCCTCTCGC TGGAGCCAGT GAGGACAAGG CTGGAACTGA GAAGATATGT 
GTCAAACAGG CTGAGGTGAA ACAGATTACC AGGCCAGCAG AGTCCAGTGA TGAGCTGCCA 
GTCCCTTCCA CTGTGACCAG CAGCAGCAAG GCCGACCAAG CAAAGGACAA AACATCAGAG 
AGTTCCTGCA GCACACGGGA TATTCTAGAG GACCACTCTC TCTGGGAATC CCCAGAAACC 
ATGTTTCGTC ACCAGACAGC ACGCTGGCCC AAGGCCTCTA TGGTGTTTGA GGTGGAGAGC 
AACCATCAGT ATCTTAATGT CGCCCTGTGG TGCAAAGATC CCTTTAAGCT TGGCAGTTTG 
TTGTGCCTGG GTCATGCCAG CCTCCAGCTG GAGCATTTAG CCATGGAATG TATGGCCACA 
TCTTCTGCAG AGTACCAGAG CACCTTTAGG TTGGGGCCTC CAGAACCCAG AGCTAACGTC 
AGCCGTACTG CACTACGTAG CCTGAGCACC CATAAGGGCT TCAATGAGAA GCTGTGTTAC 
GGAGATGTTA CTCTGAACTT CTGTTACTTA GCTGAGGGTG AATTAGAGTA TCCAGGAGGG 
CTGGCAGAAC GGGAGCGAGA GGGAAGTCTC CATGATGAAG ATCTAAGAGA GAGAGAGCGG 
GAGCATATCC CTTCAGCACC ACGTGATGAC TTGGCCTACA GCACCATGCA GTTGGTGGAG 
GTGCGTCACA ACTTCCAGGA CACCCAGTTC CAGAATCCCA CCTGGTGTGA ATACTGCAAG 
AAGAAGGTTT GGACCAAGGC TGCCTCTCAG TGTATGGTCT GCACCTATGT TTGCCACAAA 
AAGTGCCAGG ACAAGTGCCT CACTGAGAAT CCTTTCTGTG TGGCAGCAAC TGAGCGTCGT 
GGAGCTGACC CTGAAGCCAA ATCCACCATA AACCGGGCCA CCACTGGCTT AACTCGGCAT 
ATCATCAACA CCAGCTCCCG TTTGCTTAAT CTCCGCCAGG TGCCCAAGAC TCGGCTGGTT 
GAGCAGGTGG CTGATGTGGT GCCTGGAGTG GTAGAGCCCT CACCCAAGCA TACACCCAAC 
ACCTCAGACA ATGAGAGCAG TGATACTGAG ACCTACACCA GTGGCGCCAG CCCCTCAAAG 
CAGCCCTCTG GCAGCGGTGG CAGCAGTAAA CTTGTACGCA AGGAAGGTGG GTTGGATGAC 
AGTGTGTTCA TTGCTGTGAA GGAGATAGGT CGTGACCTGT ACCGGGGACT GCCCACAGAT 
GAACGCTCTC AGAAGCTTGA GTTGATGCTG GACAAATTGC AGCAGGAAAT TGACCAGGAG 
CTGGAGCACA ATAATGCTCT TCTGTTGGAG GAGAGGGAGG CCACAGATGC CCGACGCAAG 
GCCCTAATTA GTACAGCCCT GGCCAAGTCT GGGGAGAGAC TCCAGGCCCT CACCCTGCTA 
ATGATCCACT ACCGAGCAGG CATTGAGGAC CTGGAGTCAG TGGAGAGCAC CTCTCCATCA 
GAGCAGCAAG GCTTTAAGGG CAAAGGAGAA ACCCTGGAGG AAGAGGCTCT GATGGGGACA 
GAGGTCTATG ACAGTGACAT ATGCAGCCCT GTGGAGGTGC AGCTGCTGGA TGACATTACT 
GAGGAGCAAA TCTGTGTTGA GGCACTCCAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104929390 ( XM_010743901.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010742203.2 Larimichthys crocea PDZ domain-containing protein 8 (LOC104929390), mRNA.

Price & Availability↑

CloneID	OLa111566
Clone ID Related Accession (Same CDS sequence)	XM_010743901.3 , XM_010743901.2
Accession Version	XM_010743901.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	PDZ domain-containing protein 8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017609562.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 29, 2016 this sequence version replaced XM_010743901.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTACT TAATATTAAT CTCCGCGTTC TCCGGAGCAG TCGTTACGTT GCTATTACAG 
TTCTTGCTGA TCTACCGGAG AAACATCGAG CCAATAGGCA GGACAGTACA GTATGTCAAA 
GTAGTGCCTG ATAACGCACT GAAGGATTAC TTTAATACCC AACATGCCGA ACCAGGCCAG 
CACCAGCAGG ACAGCACGGC ATCCACCGCA TCCAAGCAGC CAGAGGCTGG CTCGGCCCGG 
CAGCAGGAAG CGGCTGTCAG CGGCGGCAGC CCCAAACAGC CGCCGCCTCC TCACAGCGAG 
CCCGCCGATG CGGCCAAGCC CGAGACGTGC AATTTCCTAA ACGCGATCTT TTTGTTCCTG 
TTCAGGGAGC TCCGGGACAC CCCTGTGGTC AGACACTGGC TCACCAAGAA GATCAAGGTG 
GAGTTTGAGG AGCTGCTGCA GACCAAGACA GCCGGACGGC TCCTGGAGGG ACTCAGTCTC 
AGGGATATTT CCCTGGGCAA TTCTCTGCCA GTCTTTAAGA CGGCCAAACT TATGAAGCCG 
GTGCACCTGA ACGAGGACGG CATGCCCGAG GAGCTCAACT TCGAGGTGGA CATCGAATAC 
AATGGCGGCT TCCATCTGGC CATCGACGTC GAGCTGGTGT TCGGGAAGTC TGCCTACCTG 
TTCGTTAAAA TGAGGCGCGT GGTGGGTAGG CTGAGGCTGC AGTTCACGCG CATGCCCTTC 
TCCCATTGGT CATTCTCCTT CCTCGAGGAC CCGCTGGTGG ATTTTGAGGC GAAGTCCCAG 
TTTGAAGGAA GGCCGCTGCC TCAGCTCACC TCCATCATAG TCAACCAGCT GAAGCGCGTC 
ATCAAAAAGA AACACACCTT ACCAAACTAC AAAATCAGAT ACAAGCCCTT CTTCCCGTTC 
CAAGTCCAGG CTCCGCTCGG TTCGGCTTGT GATTTGGACC TGTCAGTCCA GGACAGCAGG 
CTGGTGGAAG GCCGACTCAG AGTTACCCTC ATTGAATGTA GCAGACTGTT CATCCTGGGC 
TCCTATGACA GAGAAACCTA TGTTCACTGC ACCTTGGAGC TGAGCAGCCA CCAGTGGAAG 
GAGAAGACTC GCAGCTCCAT CAAACAGACG GAGGTTATCA AGGGGCCATG TGGCAGTGTG 
GGGATGACGT TCAGACATGT CCCTGCCAGT GAGGGGGATG CAGTGCATGT CAGCATCGAG 
ACAGTCATGC CCAACTCGCC TGCAGCCTTG GCAGACTTGC AAAGGGGTGA TCGCCTAATC 
GCCATTGGAG GTGTCAAAGT GACATCCTCA GTTCAAGTGC CCAAACTACT GAAGCAGGCT 
GGAGACAGGG TGGTGGTACT TTATGAGAGA CCTGTTCGTC ACCAGCCTCC CTCTTTTGGA 
AGCCTGGGAA CTCTTCAAGA AGGGTTTGGG CAGCTTGAGG AAACAGGTTT TATTTCCCAG 
CCAGGGGGTT ATGAAGAAGA CCCAGCCCCT ATCACCACCC TTTCTGACAT ATCCGACAGC 
AAAGACATTG ACTCTGAGTT TGAAGAGTTG ATTGTGGACT CTAGTGTGAA ACCTAGCCAG 
ACAGGTGGCC CTAACAGCAG TATCACAAAT ACCAGTGACA CTAAGGATGA TTCCTTACTT 
ACAGTAAACC AAAGCCCCAA AAGAACTGTG GCCAACTTGG CCTCTAAACC CCTTGGTACC 
ATATCACCTA TACTCAACCG CAAGTTAAAC CTGGTGGGTC TCCAGTCACC ACTAAAGCCC 
CAGCCCAAAG AATCACCGAA GCCCTCACCA CATAAGACAA GTAGTGATCC AGGGGAGGGT 
GTGCAACGCC CTACTGTCCC TCCCCCACCT CCTCCTTCTC GACCCCCAGT GCCGCCAAGA 
CCTCAGATAA GGTTGACATC AGCCTCTTCA GAAACCAGTC TTTTGGAAGG CGTTGATTCT 
GTTACAACTA CAAATGCTAC TGTTGCTCCT ACCGTAACTA CCAGCAGCTC TGAAAAATCT 
CCAGAAAAAG TTCCTCTCGC TGGAGCCAGT GAGGACAAGG CTGGAACTGA GAAGATATGT 
GTCAAACAGG CTGAGGTGAA ACAGATTACC AGGCCAGCAG AGTCCAGTGA TGAGCTGCCA 
GTCCCTTCCA CTGTGACCAG CAGCAGCAAG GCCGACCAAG CAAAGGACAA AACATCAGAG 
AGTTCCTGCA GCACACGGGA TATTCTAGAG GACCACTCTC TCTGGGAATC CCCAGAAACC 
ATGTTTCGTC ACCAGACAGC ACGCTGGCCC AAGGCCTCTA TGGTGTTTGA GGTGGAGAGC 
AACCATCAGT ATCTTAATGT CGCCCTGTGG TGCAAAGATC CCTTTAAGCT TGGCAGTTTG 
TTGTGCCTGG GTCATGCCAG CCTCCAGCTG GAGCATTTAG CCATGGAATG TATGGCCACA 
TCTTCTGCAG AGTACCAGAG CACCTTTAGG TTGGGGCCTC CAGAACCCAG AGCTAACGTC 
AGCCGTACTG CACTACGTAG CCTGAGCACC CATAAGGGCT TCAATGAGAA GCTGTGTTAC 
GGAGATGTTA CTCTGAACTT CTGTTACTTA GCTGAGGGTG AATTAGAGTA TCCAGGAGGG 
CTGGCAGAAC GGGAGCGAGA GGGAAGTCTC CATGATGAAG ATCTAAGAGA GAGAGAGCGG 
GAGCATATCC CTTCAGCACC ACGTGATGAC TTGGCCTACA GCACCATGCA GTTGGTGGAG 
GTGCGTCACA ACTTCCAGGA CACCCAGTTC CAGAATCCCA CCTGGTGTGA ATACTGCAAG 
AAGAAGGTTT GGACCAAGGC TGCCTCTCAG TGTATGGTCT GCACCTATGT TTGCCACAAA 
AAGTGCCAGG ACAAGTGCCT CACTGAGAAT CCTTTCTGTG TGGCAGCAAC TGAGCGTCGT 
GGAGCTGACC CTGAAGCCAA ATCCACCATA AACCGGGCCA CCACTGGCTT AACTCGGCAT 
ATCATCAACA CCAGCTCCCG TTTGCTTAAT CTCCGCCAGG TGCCCAAGAC TCGGCTGGTT 
GAGCAGGTGG CTGATGTGGT GCCTGGAGTG GTAGAGCCCT CACCCAAGCA TACACCCAAC 
ACCTCAGACA ATGAGAGCAG TGATACTGAG ACCTACACCA GTGGCGCCAG CCCCTCAAAG 
CAGCCCTCTG GCAGCGGTGG CAGCAGTAAA CTTGTACGCA AGGAAGGTGG GTTGGATGAC 
AGTGTGTTCA TTGCTGTGAA GGAGATAGGT CGTGACCTGT ACCGGGGACT GCCCACAGAT 
GAACGCTCTC AGAAGCTTGA GTTGATGCTG GACAAATTGC AGCAGGAAAT TGACCAGGAG 
CTGGAGCACA ATAATGCTCT TCTGTTGGAG GAGAGGGAGG CCACAGATGC CCGACGCAAG 
GCCCTAATTA GTACAGCCCT GGCCAAGTCT GGGGAGAGAC TCCAGGCCCT CACCCTGCTA 
ATGATCCACT ACCGAGCAGG CATTGAGGAC CTGGAGTCAG TGGAGAGCAC CTCTCCATCA 
GAGCAGCAAG GCTTTAAGGG CAAAGGAGAA ACCCTGGAGG AAGAGGCTCT GATGGGGACA 
GAGGTCTATG ACAGTGACAT ATGCAGCCCT GTGGAGGTGC AGCTGCTGGA TGACATTACT 
GAGGAGCAAA TCTGTGTTGA GGCACTCCAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010742203.2
CDS	340..3972
Translation	MIYLILISAFSGAVVTLLLQFLLIYRRNIEPIGRTVQYVKVVPDNALKDYFNTQHAEPGQHQQDSTASTASKQPEAGSARQQEAAVSGGSPKQPPPPHSEPADAAKPETCNFLNAIFLFLFRELRDTPVVRHWLTKKIKVEFEELLQTKTAGRLLEGLSLRDISLGNSLPVFKTAKLMKPVHLNEDGMPEELNFEVDIEYNGGFHLAIDVELVFGKSAYLFVKMRRVVGRLRLQFTRMPFSHWSFSFLEDPLVDFEAKSQFEGRPLPQLTSIIVNQLKRVIKKKHTLPNYKIRYKPFFPFQVQAPLGSACDLDLSVQDSRLVEGRLRVTLIECSRLFILGSYDRETYVHCTLELSSHQWKEKTRSSIKQTEVIKGPCGSVGMTFRHVPASEGDAVHVSIETVMPNSPAALADLQRGDRLIAIGGVKVTSSVQVPKLLKQAGDRVVVLYERPVRHQPPSFGSLGTLQEGFGQLEETGFISQPGGYEEDPAPITTLSDISDSKDIDSEFEELIVDSSVKPSQTGGPNSSITNTSDTKDDSLLTVNQSPKRTVANLASKPLGTISPILNRKLNLVGLQSPLKPQPKESPKPSPHKTSSDPGEGVQRPTVPPPPPPSRPPVPPRPQIRLTSASSETSLLEGVDSVTTTNATVAPTVTTSSSEKSPEKVPLAGASEDKAGTEKICVKQAEVKQITRPAESSDELPVPSTVTSSSKADQAKDKTSESSCSTRDILEDHSLWESPETMFRHQTARWPKASMVFEVESNHQYLNVALWCKDPFKLGSLLCLGHASLQLEHLAMECMATSSAEYQSTFRLGPPEPRANVSRTALRSLSTHKGFNEKLCYGDVTLNFCYLAEGELEYPGGLAEREREGSLHDEDLREREREHIPSAPRDDLAYSTMQLVEVRHNFQDTQFQNPTWCEYCKKKVWTKAASQCMVCTYVCHKKCQDKCLTENPFCVAATERRGADPEAKSTINRATTGLTRHIINTSSRLLNLRQVPKTRLVEQVADVVPGVVEPSPKHTPNTSDNESSDTETYTSGASPSKQPSGSGGSSKLVRKEGGLDDSVFIAVKEIGRDLYRGLPTDERSQKLELMLDKLQQEIDQELEHNNALLLEEREATDARRKALISTALAKSGERLQALTLLMIHYRAGIEDLESVESTSPSEQQGFKGKGETLEEEALMGTEVYDSDICSPVEVQLLDDITEEQICVEALH

Target ORF information:

RefSeq Version	XM_010743901.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea PDZ domain-containing protein 8 (LOC104929390), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010743901.2

ORF Insert Sequence:

ATGATTTACT TAATATTAAT CTCCGCGTTC TCCGGAGCAG TCGTTACGTT GCTATTACAG 
TTCTTGCTGA TCTACCGGAG AAACATCGAG CCAATAGGCA GGACAGTACA GTATGTCAAA 
GTAGTGCCTG ATAACGCACT GAAGGATTAC TTTAATACCC AACATGCCGA ACCAGGCCAG 
CACCAGCAGG ACAGCACGGC ATCCACCGCA TCCAAGCAGC CAGAGGCTGG CTCGGCCCGG 
CAGCAGGAAG CGGCTGTCAG CGGCGGCAGC CCCAAACAGC CGCCGCCTCC TCACAGCGAG 
CCCGCCGATG CGGCCAAGCC CGAGACGTGC AATTTCCTAA ACGCGATCTT TTTGTTCCTG 
TTCAGGGAGC TCCGGGACAC CCCTGTGGTC AGACACTGGC TCACCAAGAA GATCAAGGTG 
GAGTTTGAGG AGCTGCTGCA GACCAAGACA GCCGGACGGC TCCTGGAGGG ACTCAGTCTC 
AGGGATATTT CCCTGGGCAA TTCTCTGCCA GTCTTTAAGA CGGCCAAACT TATGAAGCCG 
GTGCACCTGA ACGAGGACGG CATGCCCGAG GAGCTCAACT TCGAGGTGGA CATCGAATAC 
AATGGCGGCT TCCATCTGGC CATCGACGTC GAGCTGGTGT TCGGGAAGTC TGCCTACCTG 
TTCGTTAAAA TGAGGCGCGT GGTGGGTAGG CTGAGGCTGC AGTTCACGCG CATGCCCTTC 
TCCCATTGGT CATTCTCCTT CCTCGAGGAC CCGCTGGTGG ATTTTGAGGC GAAGTCCCAG 
TTTGAAGGAA GGCCGCTGCC TCAGCTCACC TCCATCATAG TCAACCAGCT GAAGCGCGTC 
ATCAAAAAGA AACACACCTT ACCAAACTAC AAAATCAGAT ACAAGCCCTT CTTCCCGTTC 
CAAGTCCAGG CTCCGCTCGG TTCGGCTTGT GATTTGGACC TGTCAGTCCA GGACAGCAGG 
CTGGTGGAAG GCCGACTCAG AGTTACCCTC ATTGAATGTA GCAGACTGTT CATCCTGGGC 
TCCTATGACA GAGAAACCTA TGTTCACTGC ACCTTGGAGC TGAGCAGCCA CCAGTGGAAG 
GAGAAGACTC GCAGCTCCAT CAAACAGACG GAGGTTATCA AGGGGCCATG TGGCAGTGTG 
GGGATGACGT TCAGACATGT CCCTGCCAGT GAGGGGGATG CAGTGCATGT CAGCATCGAG 
ACAGTCATGC CCAACTCGCC TGCAGCCTTG GCAGACTTGC AAAGGGGTGA TCGCCTAATC 
GCCATTGGAG GTGTCAAAGT GACATCCTCA GTTCAAGTGC CCAAACTACT GAAGCAGGCT 
GGAGACAGGG TGGTGGTACT TTATGAGAGA CCTGTTCGTC ACCAGCCTCC CTCTTTTGGA 
AGCCTGGGAA CTCTTCAAGA AGGGTTTGGG CAGCTTGAGG AAACAGGTTT TATTTCCCAG 
CCAGGGGGTT ATGAAGAAGA CCCAGCCCCT ATCACCACCC TTTCTGACAT ATCCGACAGC 
AAAGACATTG ACTCTGAGTT TGAAGAGTTG ATTGTGGACT CTAGTGTGAA ACCTAGCCAG 
ACAGGTGGCC CTAACAGCAG TATCACAAAT ACCAGTGACA CTAAGGATGA TTCCTTACTT 
ACAGTAAACC AAAGCCCCAA AAGAACTGTG GCCAACTTGG CCTCTAAACC CCTTGGTACC 
ATATCACCTA TACTCAACCG CAAGTTAAAC CTGGTGGGTC TCCAGTCACC ACTAAAGCCC 
CAGCCCAAAG AATCACCGAA GCCCTCACCA CATAAGACAA GTAGTGATCC AGGGGAGGGT 
GTGCAACGCC CTACTGTCCC TCCCCCACCT CCTCCTTCTC GACCCCCAGT GCCGCCAAGA 
CCTCAGATAA GGTTGACATC AGCCTCTTCA GAAACCAGTC TTTTGGAAGG CGTTGATTCT 
GTTACAACTA CAAATGCTAC TGTTGCTCCT ACCGTAACTA CCAGCAGCTC TGAAAAATCT 
CCAGAAAAAG TTCCTCTCGC TGGAGCCAGT GAGGACAAGG CTGGAACTGA GAAGATATGT 
GTCAAACAGG CTGAGGTGAA ACAGATTACC AGGCCAGCAG AGTCCAGTGA TGAGCTGCCA 
GTCCCTTCCA CTGTGACCAG CAGCAGCAAG GCCGACCAAG CAAAGGACAA AACATCAGAG 
AGTTCCTGCA GCACACGGGA TATTCTAGAG GACCACTCTC TCTGGGAATC CCCAGAAACC 
ATGTTTCGTC ACCAGACAGC ACGCTGGCCC AAGGCCTCTA TGGTGTTTGA GGTGGAGAGC 
AACCATCAGT ATCTTAATGT CGCCCTGTGG TGCAAAGATC CCTTTAAGCT TGGCAGTTTG 
TTGTGCCTGG GTCATGCCAG CCTCCAGCTG GAGCATTTAG CCATGGAATG TATGGCCACA 
TCTTCTGCAG AGTACCAGAG CACCTTTAGG TTGGGGCCTC CAGAACCCAG AGCTAACGTC 
AGCCGTACTG CACTACGTAG CCTGAGCACC CATAAGGGCT TCAATGAGAA GCTGTGTTAC 
GGAGATGTTA CTCTGAACTT CTGTTACTTA GCTGAGGGTG AATTAGAGTA TCCAGGAGGG 
CTGGCAGAAC GGGAGCGAGA GGGAAGTCTC CATGATGAAG ATCTAAGAGA GAGAGAGCGG 
GAGCATATCC CTTCAGCACC ACGTGATGAC TTGGCCTACA GCACCATGCA GTTGGTGGAG 
GTGCGTCACA ACTTCCAGGA CACCCAGTTC CAGAATCCCA CCTGGTGTGA ATACTGCAAG 
AAGAAGGTTT GGACCAAGGC TGCCTCTCAG TGTATGGTCT GCACCTATGT TTGCCACAAA 
AAGTGCCAGG ACAAGTGCCT CACTGAGAAT CCTTTCTGTG TGGCAGCAAC TGAGCGTCGT 
GGAGCTGACC CTGAAGCCAA ATCCACCATA AACCGGGCCA CCACTGGCTT AACTCGGCAT 
ATCATCAACA CCAGCTCCCG TTTGCTTAAT CTCCGCCAGG TGCCCAAGAC TCGGCTGGTT 
GAGCAGGTGG CTGATGTGGT GCCTGGAGTG GTAGAGCCCT CACCCAAGCA TACACCCAAC 
ACCTCAGACA ATGAGAGCAG TGATACTGAG ACCTACACCA GTGGCGCCAG CCCCTCAAAG 
CAGCCCTCTG GCAGCGGTGG CAGCAGTAAA CTTGTACGCA AGGAAGGTGG GTTGGATGAC 
AGTGTGTTCA TTGCTGTGAA GGAGATAGGT CGTGACCTGT ACCGGGGACT GCCCACAGAT 
GAACGCTCTC AGAAGCTTGA GTTGATGCTG GACAAATTGC AGCAGGAAAT TGACCAGGAG 
CTGGAGCACA ATAATGCTCT TCTGTTGGAG GAGAGGGAGG CCACAGATGC CCGACGCAAG 
GCCCTAATTA GTACAGCCCT GGCCAAGTCT GGGGAGAGAC TCCAGGCCCT CACCCTGCTA 
ATGATCCACT ACCGAGCAGG CATTGAGGAC CTGGAGTCAG TGGAGAGCAC CTCTCCATCA 
GAGCAGCAAG GCTTTAAGGG CAAAGGAGAA ACCCTGGAGG AAGAGGCTCT GATGGGGACA 
GAGGTCTATG ACAGTGACAT ATGCAGCCCT GTGGAGGTGC AGCTGCTGGA TGACATTACT 
GAGGAGCAAA TCTGTGTTGA GGCACTCCAC TAA