lrrc8a cDNA ORF clone, Larimichthys crocea(large yellow croaker)

Gene
Clones
gRNAs

Summary

Gene Symbol	lrrc8a
Entrez Gene ID	104926519
Full Name	leucine rich repeat containing 8 VRAC subunit A
Gene Type	protein-coding
Organism	Larimichthys crocea(large yellow croaker)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010740392.3	XP_010738694.1	volume-regulated anion channel subunit LRRC8A
XM_010740392.2	XP_010738694.1	volume-regulated anion channel subunit LRRC8A

Pathways from BioSystems
Gene Ontology

The following lrrc8a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lrrc8a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLa112824	XM_010740392.2 Latest version!	Larimichthys crocea leucine rich repeat containing 8 family member A (lrrc8a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OLa224198	XM_010740392.3 Latest version!	Larimichthys crocea leucine rich repeat containing 8 VRAC subunit A (LOC104926519), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lrrc8a ( XM_010740392.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010738694.1 Larimichthys crocea leucine rich repeat containing 8 family member A (lrrc8a), mRNA.

Price & Availability↑

CloneID	OLa112824
Clone ID Related Accession (Same CDS sequence)	XM_010740392.2
Accession Version	XM_010740392.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	volume-regulated anion channel subunit LRRC8A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017609670.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 29, 2016 this sequence version replaced XM_010740392.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCCCA TTACTGAGCT CCGGTACTTT GCTGACACCC AGCCAGCGTA CCGTATCCTG 
AAGCCATGGT GGGACGTTTT CACCGACTAC ATCTCAATCG TCATGCTGAT GATTGCGGTG 
TTCGGTGGTA CGCTGCAGGT CACGCAGGAC AAGATGATCT GCCTGCCTTG CAAGTGGGTG 
GTCAACAAGT CGTGCGAGAC CATGCCCGTG CCAAATGTGA GCACCGCCTA TGCACCAGAA 
CCCAAAGGCA TTCAGTATGA CCTCGACCGT CATCAGTATA ACTATGTCGA TGCCGTCTGC 
TATGAGAACA AACTGCACTG GTTTGCCAAA TATTTCCCCT ATCTGGTGCT ACTCCACACT 
CTTATCTTTC TGGCCTGCAG CAACTTCTGG TTCAAGTTCC CCCGCACAAG CTCTAAACTG 
GAGCACTTTG TCTCCATCCT CCTCAAGTGC TTTGACTCAC CCTGGACAAC AAGGGCTTTG 
TCTGAGACGG TGGTGGAGGA GAGCGACTCC AAGCCTCTGG GAAAAATGAA CGGCTCGATG 
GACAAGAAGG CCTCGTGCGT GAGCGAAGAT GTCGAGGCCA GCGTGCCCAT GCTCCAACGA 
ACAAAGTCCA GGATCGAACA AGGAATCGTG GATCGCTCTG AAACTGGGGT TTTGGATAAG 
AAGGAAGGGG AGCAGGCCAA AGCTCTTTTT GAGAAGGTGA AGAAGTTCAG GATCCACGTA 
GAGGAAGGCG ATATAGTGTA TCGTCTTTAC ATACGTCAGA CCATCATCAA AGTAATCAAG 
TTTATACTGA TAATTAGCTA CACGGTCAAT TATGTACGCT ACATCAGGTT CAGCGTAGTT 
TGCACGGTGA ACATTCAGAA ACTAACAGGG TACAGCACGT TCTATTGCGC TCACCCACTG 
GCAACTCTTT TCAAGATTCT GGCCTGTTTC TACATCAGTT TGGTGGTGGT CTATGGGCTT 
ATCTGCATGT ACACCCTCTG TTGGATGGTC AGTCGCTCCC TCAAACGATA TTCCTTCGAA 
TCAATCCGCG AGGAAAGCAG CTACAGCGAC ATCCCCGACC TGAAGAACGA CTTTGCCTTC 
ATGCTACACA TGATAGATCA GTACGATCCT CTCTACTCCA AGCGCTTCGC TGTCTTTCTG 
TCGGAAGTGA GCGAGAACAA GCTGAGGCAG CTGAACCTGA ACAACGAGTG GACGCTGGAG 
AAGCTGAGGC AGCGCATCAC CAAGAACTCG CAGGAGAAGC TGGAGCTGCA TCTGTTCATG 
CTCAGCGGGA TCCCGGACAC AGTATTTGAT CTGATCGAGC TGGAGGTGCT CAAGCTGGAG 
CTTATCCCCG ACGTGACTAT CCCACCGATC ATCGCCCAGC TCTCCAACCT GAGGGAGATG 
TGGCTCTATC ACACACCAGC TAAAATCGAG GCTCCGGCTC TTGCTTTCCT GAGAGAGAAC 
CTGAAGTCTC TTCACATCAA GTTCACGGAC ATCAAGGAGA TCCCACTGTG GATCTACAGC 
CTGAAGAACC TCAGTGAGCT GCATCTAACC GGGAACCTGA GCGCTGAGAA CAATCGGTAC 
ATCGTCATCG ACGGGCTTCG GGAGCTCAAA AGGCTCAAAG TGTTACGTCT GAAAAGCAAC 
CTGACCAAGC TGCCGCAGGT GGTGACGGAC GTGGGCGTGC ACCTCCAGAA GCTCTCCATC 
AATAACGAGG GCACCAAGCT GATGGTGCTC AACAGCCTGA AGAAAATGGT CAACCTGACG 
GAGCTCGAGC TGGTTCGCTG CGATCTCGAA CGCGTCCCGC ACTCCATCTT CAGTCTGCAC 
AACCTGCAGG AGATCGACCT GAAGGACAAC AACCTGAAGA CGATAGAGGA GATCATCAGC 
TTTCAGCACC TGCACCGGCT CGTGTGCCTG AAGCTGTGGT ACAACCAGAT CGCTTACATC 
CCCATTCAGA TCGGAACGCT CACCAACCTG GAGAGGCTGT ACCTGAACAG GAACAAGATC 
GAGAAAATCC CCAGCCAGCT CTTCTTCTGT CGCAAGCTGC GCTTCTTAGA CCTGAGCCAC 
AACAATCTGA CCAGCATCCA CGCCGATGTA GGCTTCCTCC AAAACCTGCA GTACTTTGCT 
GTGACGGCAA ACAGGATCGA GACTTTGCCC CCGGAGCTGT TCAAGTGCAA GAAGCTGCGC 
ACTCTGAATC TGGGAAACAA CTGCCTGCAG ACGCTGCCGT CGCGCTTCGG AGAGCTCACC 
GGGTTGACGC AGCTGGAGCT GAGAGGGAAC CGTCTCGAGT GTCTCCCCGT GGAGCTCGGC 
GAGTGTCGCC TGCTGAAGAG GAGCGGCCTG GTGGTGGAGG AGGACCTGTT CAACACGCTG 
CCGTCGGAAG TCAAAGAGCA GCTCTGGAGG GCCGATAAGG AGCAGGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010738694.1
CDS	219..2609
Translation	MIPITELRYFADTQPAYRILKPWWDVFTDYISIVMLMIAVFGGTLQVTQDKMICLPCKWVVNKSCETMPVPNVSTAYAPEPKGIQYDLDRHQYNYVDAVCYENKLHWFAKYFPYLVLLHTLIFLACSNFWFKFPRTSSKLEHFVSILLKCFDSPWTTRALSETVVEESDSKPLGKMNGSMDKKASCVSEDVEASVPMLQRTKSRIEQGIVDRSETGVLDKKEGEQAKALFEKVKKFRIHVEEGDIVYRLYIRQTIIKVIKFILIISYTVNYVRYIRFSVVCTVNIQKLTGYSTFYCAHPLATLFKILACFYISLVVVYGLICMYTLCWMVSRSLKRYSFESIREESSYSDIPDLKNDFAFMLHMIDQYDPLYSKRFAVFLSEVSENKLRQLNLNNEWTLEKLRQRITKNSQEKLELHLFMLSGIPDTVFDLIELEVLKLELIPDVTIPPIIAQLSNLREMWLYHTPAKIEAPALAFLRENLKSLHIKFTDIKEIPLWIYSLKNLSELHLTGNLSAENNRYIVIDGLRELKRLKVLRLKSNLTKLPQVVTDVGVHLQKLSINNEGTKLMVLNSLKKMVNLTELELVRCDLERVPHSIFSLHNLQEIDLKDNNLKTIEEIISFQHLHRLVCLKLWYNQIAYIPIQIGTLTNLERLYLNRNKIEKIPSQLFFCRKLRFLDLSHNNLTSIHADVGFLQNLQYFAVTANRIETLPPELFKCKKLRTLNLGNNCLQTLPSRFGELTGLTQLELRGNRLECLPVELGECRLLKRSGLVVEEDLFNTLPSEVKEQLWRADKEQA

Target ORF information:

RefSeq Version	XM_010740392.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea leucine rich repeat containing 8 family member A (lrrc8a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010740392.2

ORF Insert Sequence:

ATGATTCCCA TTACTGAGCT CCGGTACTTT GCTGACACCC AGCCAGCGTA CCGTATCCTG 
AAGCCATGGT GGGACGTTTT CACCGACTAC ATCTCAATCG TCATGCTGAT GATTGCGGTG 
TTCGGTGGTA CGCTGCAGGT CACGCAGGAC AAGATGATCT GCCTGCCTTG CAAGTGGGTG 
GTCAACAAGT CGTGCGAGAC CATGCCCGTG CCAAATGTGA GCACCGCCTA TGCACCAGAA 
CCCAAAGGCA TTCAGTATGA CCTCGACCGT CATCAGTATA ACTATGTCGA TGCCGTCTGC 
TATGAGAACA AACTGCACTG GTTTGCCAAA TATTTCCCCT ATCTGGTGCT ACTCCACACT 
CTTATCTTTC TGGCCTGCAG CAACTTCTGG TTCAAGTTCC CCCGCACAAG CTCTAAACTG 
GAGCACTTTG TCTCCATCCT CCTCAAGTGC TTTGACTCAC CCTGGACAAC AAGGGCTTTG 
TCTGAGACGG TGGTGGAGGA GAGCGACTCC AAGCCTCTGG GAAAAATGAA CGGCTCGATG 
GACAAGAAGG CCTCGTGCGT GAGCGAAGAT GTCGAGGCCA GCGTGCCCAT GCTCCAACGA 
ACAAAGTCCA GGATCGAACA AGGAATCGTG GATCGCTCTG AAACTGGGGT TTTGGATAAG 
AAGGAAGGGG AGCAGGCCAA AGCTCTTTTT GAGAAGGTGA AGAAGTTCAG GATCCACGTA 
GAGGAAGGCG ATATAGTGTA TCGTCTTTAC ATACGTCAGA CCATCATCAA AGTAATCAAG 
TTTATACTGA TAATTAGCTA CACGGTCAAT TATGTACGCT ACATCAGGTT CAGCGTAGTT 
TGCACGGTGA ACATTCAGAA ACTAACAGGG TACAGCACGT TCTATTGCGC TCACCCACTG 
GCAACTCTTT TCAAGATTCT GGCCTGTTTC TACATCAGTT TGGTGGTGGT CTATGGGCTT 
ATCTGCATGT ACACCCTCTG TTGGATGGTC AGTCGCTCCC TCAAACGATA TTCCTTCGAA 
TCAATCCGCG AGGAAAGCAG CTACAGCGAC ATCCCCGACC TGAAGAACGA CTTTGCCTTC 
ATGCTACACA TGATAGATCA GTACGATCCT CTCTACTCCA AGCGCTTCGC TGTCTTTCTG 
TCGGAAGTGA GCGAGAACAA GCTGAGGCAG CTGAACCTGA ACAACGAGTG GACGCTGGAG 
AAGCTGAGGC AGCGCATCAC CAAGAACTCG CAGGAGAAGC TGGAGCTGCA TCTGTTCATG 
CTCAGCGGGA TCCCGGACAC AGTATTTGAT CTGATCGAGC TGGAGGTGCT CAAGCTGGAG 
CTTATCCCCG ACGTGACTAT CCCACCGATC ATCGCCCAGC TCTCCAACCT GAGGGAGATG 
TGGCTCTATC ACACACCAGC TAAAATCGAG GCTCCGGCTC TTGCTTTCCT GAGAGAGAAC 
CTGAAGTCTC TTCACATCAA GTTCACGGAC ATCAAGGAGA TCCCACTGTG GATCTACAGC 
CTGAAGAACC TCAGTGAGCT GCATCTAACC GGGAACCTGA GCGCTGAGAA CAATCGGTAC 
ATCGTCATCG ACGGGCTTCG GGAGCTCAAA AGGCTCAAAG TGTTACGTCT GAAAAGCAAC 
CTGACCAAGC TGCCGCAGGT GGTGACGGAC GTGGGCGTGC ACCTCCAGAA GCTCTCCATC 
AATAACGAGG GCACCAAGCT GATGGTGCTC AACAGCCTGA AGAAAATGGT CAACCTGACG 
GAGCTCGAGC TGGTTCGCTG CGATCTCGAA CGCGTCCCGC ACTCCATCTT CAGTCTGCAC 
AACCTGCAGG AGATCGACCT GAAGGACAAC AACCTGAAGA CGATAGAGGA GATCATCAGC 
TTTCAGCACC TGCACCGGCT CGTGTGCCTG AAGCTGTGGT ACAACCAGAT CGCTTACATC 
CCCATTCAGA TCGGAACGCT CACCAACCTG GAGAGGCTGT ACCTGAACAG GAACAAGATC 
GAGAAAATCC CCAGCCAGCT CTTCTTCTGT CGCAAGCTGC GCTTCTTAGA CCTGAGCCAC 
AACAATCTGA CCAGCATCCA CGCCGATGTA GGCTTCCTCC AAAACCTGCA GTACTTTGCT 
GTGACGGCAA ACAGGATCGA GACTTTGCCC CCGGAGCTGT TCAAGTGCAA GAAGCTGCGC 
ACTCTGAATC TGGGAAACAA CTGCCTGCAG ACGCTGCCGT CGCGCTTCGG AGAGCTCACC 
GGGTTGACGC AGCTGGAGCT GAGAGGGAAC CGTCTCGAGT GTCTCCCCGT GGAGCTCGGC 
GAGTGTCGCC TGCTGAAGAG GAGCGGCCTG GTGGTGGAGG AGGACCTGTT CAACACGCTG 
CCGTCGGAAG TCAAAGAGCA GCTCTGGAGG GCCGATAAGG AGCAGGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

lrrc8a ( XM_010740392.3 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010738694.1 Larimichthys crocea leucine rich repeat containing 8 VRAC subunit A (LOC104926519), mRNA.

Price & Availability↑

CloneID	OLa224198
Clone ID Related Accession (Same CDS sequence)	XM_010740392.3
Accession Version	XM_010740392.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	volume-regulated anion channel subunit LRRC8A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040019.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_010740392.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTCCCA TTACTGAGCT CCGGTACTTT GCTGACACCC AGCCAGCGTA CCGTATCCTG 
AAGCCATGGT GGGACGTTTT CACCGACTAC ATCTCAATCG TCATGCTGAT GATTGCGGTG 
TTCGGTGGTA CGCTGCAGGT CACGCAGGAC AAGATGATCT GCCTGCCTTG CAAGTGGGTG 
GTCAACAAGT CGTGCGAGAC CATGCCCGTG CCAAATGTGA GCACCGCCTA TGCACCAGAA 
CCCAAAGGCA TTCAGTATGA CCTCGACCGT CATCAGTATA ACTATGTCGA TGCCGTCTGC 
TATGAGAACA AACTGCACTG GTTTGCCAAA TATTTCCCCT ATCTGGTGCT ACTCCACACT 
CTTATCTTTC TGGCCTGCAG CAACTTCTGG TTCAAGTTCC CCCGCACAAG CTCTAAACTG 
GAGCACTTTG TCTCCATCCT CCTCAAGTGC TTTGACTCAC CCTGGACAAC AAGGGCTTTG 
TCTGAGACGG TGGTGGAGGA GAGCGACTCC AAGCCTCTGG GAAAAATGAA CGGCTCGATG 
GACAAGAAGG CCTCGTGCGT GAGCGAAGAT GTCGAGGCCA GCGTGCCCAT GCTCCAACGA 
ACAAAGTCCA GGATCGAACA AGGAATCGTG GATCGCTCTG AAACTGGGGT TTTGGATAAG 
AAGGAAGGGG AGCAGGCCAA AGCTCTTTTT GAGAAGGTGA AGAAGTTCAG GATCCACGTA 
GAGGAAGGCG ATATAGTGTA TCGTCTTTAC ATACGTCAGA CCATCATCAA AGTAATCAAG 
TTTATACTGA TAATTAGCTA CACGGTCAAT TATGTACGCT ACATCAGGTT CAGCGTAGTT 
TGCACGGTGA ACATTCAGAA ACTAACAGGG TACAGCACGT TCTATTGCGC TCACCCACTG 
GCAACTCTTT TCAAGATTCT GGCCTGTTTC TACATCAGTT TGGTGGTGGT CTATGGGCTT 
ATCTGCATGT ACACCCTCTG TTGGATGGTC AGTCGCTCCC TCAAACGATA TTCCTTCGAA 
TCAATCCGCG AGGAAAGCAG CTACAGCGAC ATCCCCGACC TGAAGAACGA CTTTGCCTTC 
ATGCTACACA TGATAGATCA GTACGATCCT CTCTACTCCA AGCGCTTCGC TGTCTTTCTG 
TCGGAAGTGA GCGAGAACAA GCTGAGGCAG CTGAACCTGA ACAACGAGTG GACGCTGGAG 
AAGCTGAGGC AGCGCATCAC CAAGAACTCG CAGGAGAAGC TGGAGCTGCA TCTGTTCATG 
CTCAGCGGGA TCCCGGACAC AGTATTTGAT CTGATCGAGC TGGAGGTGCT CAAGCTGGAG 
CTTATCCCCG ACGTGACTAT CCCACCGATC ATCGCCCAGC TCTCCAACCT GAGGGAGATG 
TGGCTCTATC ACACACCAGC TAAAATCGAG GCTCCGGCTC TTGCTTTCCT GAGAGAGAAC 
CTGAAGTCTC TTCACATCAA GTTCACGGAC ATCAAGGAGA TCCCACTGTG GATCTACAGC 
CTGAAGAACC TCAGTGAGCT GCATCTAACC GGGAACCTGA GCGCTGAGAA CAATCGGTAC 
ATCGTCATCG ACGGGCTTCG GGAGCTCAAA AGGCTCAAAG TGTTACGTCT GAAAAGCAAC 
CTGACCAAGC TGCCGCAGGT GGTGACGGAC GTGGGCGTGC ACCTCCAGAA GCTCTCCATC 
AATAACGAGG GCACCAAGCT GATGGTGCTC AACAGCCTGA AGAAAATGGT CAACCTGACG 
GAGCTCGAGC TGGTTCGCTG CGATCTCGAA CGCGTCCCGC ACTCCATCTT CAGTCTGCAC 
AACCTGCAGG AGATCGACCT GAAGGACAAC AACCTGAAGA CGATAGAGGA GATCATCAGC 
TTTCAGCACC TGCACCGGCT CGTGTGCCTG AAGCTGTGGT ACAACCAGAT CGCTTACATC 
CCCATTCAGA TCGGAACGCT CACCAACCTG GAGAGGCTGT ACCTGAACAG GAACAAGATC 
GAGAAAATCC CCAGCCAGCT CTTCTTCTGT CGCAAGCTGC GCTTCTTAGA CCTGAGCCAC 
AACAATCTGA CCAGCATCCA CGCCGATGTA GGCTTCCTCC AGAACCTGCA GTACTTCGCC 
GTGACGGCAA ACAGGATCGA GACTTTGCCC CCGGAGCTGT TCAAGTGCAA GAAGCTGCGC 
ACTCTGAATC TGGGAAACAA CTGCCTGCAG ACGCTGCCGT CGCGCTTCGG AGAGCTCACC 
GGGTTGACGC AGCTGGAGCT GAGAGGGAAC CGTCTCGAGT GTCTCCCCGT GGAGCTCGGC 
GAGTGTCGCC TGCTGAAGAG GAGCGGCCTG GTGGTGGAGG AGGACCTGTT CAACACGCTG 
CCGTCGGAAG TCAAAGAGCA GCTCTGGAGG GCCGATAAGG AGCAGGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010738694.1
CDS	222..2612
Translation	MIPITELRYFADTQPAYRILKPWWDVFTDYISIVMLMIAVFGGTLQVTQDKMICLPCKWVVNKSCETMPVPNVSTAYAPEPKGIQYDLDRHQYNYVDAVCYENKLHWFAKYFPYLVLLHTLIFLACSNFWFKFPRTSSKLEHFVSILLKCFDSPWTTRALSETVVEESDSKPLGKMNGSMDKKASCVSEDVEASVPMLQRTKSRIEQGIVDRSETGVLDKKEGEQAKALFEKVKKFRIHVEEGDIVYRLYIRQTIIKVIKFILIISYTVNYVRYIRFSVVCTVNIQKLTGYSTFYCAHPLATLFKILACFYISLVVVYGLICMYTLCWMVSRSLKRYSFESIREESSYSDIPDLKNDFAFMLHMIDQYDPLYSKRFAVFLSEVSENKLRQLNLNNEWTLEKLRQRITKNSQEKLELHLFMLSGIPDTVFDLIELEVLKLELIPDVTIPPIIAQLSNLREMWLYHTPAKIEAPALAFLRENLKSLHIKFTDIKEIPLWIYSLKNLSELHLTGNLSAENNRYIVIDGLRELKRLKVLRLKSNLTKLPQVVTDVGVHLQKLSINNEGTKLMVLNSLKKMVNLTELELVRCDLERVPHSIFSLHNLQEIDLKDNNLKTIEEIISFQHLHRLVCLKLWYNQIAYIPIQIGTLTNLERLYLNRNKIEKIPSQLFFCRKLRFLDLSHNNLTSIHADVGFLQNLQYFAVTANRIETLPPELFKCKKLRTLNLGNNCLQTLPSRFGELTGLTQLELRGNRLECLPVELGECRLLKRSGLVVEEDLFNTLPSEVKEQLWRADKEQA

Target ORF information:

RefSeq Version	XM_010740392.3
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea leucine rich repeat containing 8 VRAC subunit A (LOC104926519), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010740392.3

ORF Insert Sequence:

ATGATTCCCA TTACTGAGCT CCGGTACTTT GCTGACACCC AGCCAGCGTA CCGTATCCTG 
AAGCCATGGT GGGACGTTTT CACCGACTAC ATCTCAATCG TCATGCTGAT GATTGCGGTG 
TTCGGTGGTA CGCTGCAGGT CACGCAGGAC AAGATGATCT GCCTGCCTTG CAAGTGGGTG 
GTCAACAAGT CGTGCGAGAC CATGCCCGTG CCAAATGTGA GCACCGCCTA TGCACCAGAA 
CCCAAAGGCA TTCAGTATGA CCTCGACCGT CATCAGTATA ACTATGTCGA TGCCGTCTGC 
TATGAGAACA AACTGCACTG GTTTGCCAAA TATTTCCCCT ATCTGGTGCT ACTCCACACT 
CTTATCTTTC TGGCCTGCAG CAACTTCTGG TTCAAGTTCC CCCGCACAAG CTCTAAACTG 
GAGCACTTTG TCTCCATCCT CCTCAAGTGC TTTGACTCAC CCTGGACAAC AAGGGCTTTG 
TCTGAGACGG TGGTGGAGGA GAGCGACTCC AAGCCTCTGG GAAAAATGAA CGGCTCGATG 
GACAAGAAGG CCTCGTGCGT GAGCGAAGAT GTCGAGGCCA GCGTGCCCAT GCTCCAACGA 
ACAAAGTCCA GGATCGAACA AGGAATCGTG GATCGCTCTG AAACTGGGGT TTTGGATAAG 
AAGGAAGGGG AGCAGGCCAA AGCTCTTTTT GAGAAGGTGA AGAAGTTCAG GATCCACGTA 
GAGGAAGGCG ATATAGTGTA TCGTCTTTAC ATACGTCAGA CCATCATCAA AGTAATCAAG 
TTTATACTGA TAATTAGCTA CACGGTCAAT TATGTACGCT ACATCAGGTT CAGCGTAGTT 
TGCACGGTGA ACATTCAGAA ACTAACAGGG TACAGCACGT TCTATTGCGC TCACCCACTG 
GCAACTCTTT TCAAGATTCT GGCCTGTTTC TACATCAGTT TGGTGGTGGT CTATGGGCTT 
ATCTGCATGT ACACCCTCTG TTGGATGGTC AGTCGCTCCC TCAAACGATA TTCCTTCGAA 
TCAATCCGCG AGGAAAGCAG CTACAGCGAC ATCCCCGACC TGAAGAACGA CTTTGCCTTC 
ATGCTACACA TGATAGATCA GTACGATCCT CTCTACTCCA AGCGCTTCGC TGTCTTTCTG 
TCGGAAGTGA GCGAGAACAA GCTGAGGCAG CTGAACCTGA ACAACGAGTG GACGCTGGAG 
AAGCTGAGGC AGCGCATCAC CAAGAACTCG CAGGAGAAGC TGGAGCTGCA TCTGTTCATG 
CTCAGCGGGA TCCCGGACAC AGTATTTGAT CTGATCGAGC TGGAGGTGCT CAAGCTGGAG 
CTTATCCCCG ACGTGACTAT CCCACCGATC ATCGCCCAGC TCTCCAACCT GAGGGAGATG 
TGGCTCTATC ACACACCAGC TAAAATCGAG GCTCCGGCTC TTGCTTTCCT GAGAGAGAAC 
CTGAAGTCTC TTCACATCAA GTTCACGGAC ATCAAGGAGA TCCCACTGTG GATCTACAGC 
CTGAAGAACC TCAGTGAGCT GCATCTAACC GGGAACCTGA GCGCTGAGAA CAATCGGTAC 
ATCGTCATCG ACGGGCTTCG GGAGCTCAAA AGGCTCAAAG TGTTACGTCT GAAAAGCAAC 
CTGACCAAGC TGCCGCAGGT GGTGACGGAC GTGGGCGTGC ACCTCCAGAA GCTCTCCATC 
AATAACGAGG GCACCAAGCT GATGGTGCTC AACAGCCTGA AGAAAATGGT CAACCTGACG 
GAGCTCGAGC TGGTTCGCTG CGATCTCGAA CGCGTCCCGC ACTCCATCTT CAGTCTGCAC 
AACCTGCAGG AGATCGACCT GAAGGACAAC AACCTGAAGA CGATAGAGGA GATCATCAGC 
TTTCAGCACC TGCACCGGCT CGTGTGCCTG AAGCTGTGGT ACAACCAGAT CGCTTACATC 
CCCATTCAGA TCGGAACGCT CACCAACCTG GAGAGGCTGT ACCTGAACAG GAACAAGATC 
GAGAAAATCC CCAGCCAGCT CTTCTTCTGT CGCAAGCTGC GCTTCTTAGA CCTGAGCCAC 
AACAATCTGA CCAGCATCCA CGCCGATGTA GGCTTCCTCC AGAACCTGCA GTACTTCGCC 
GTGACGGCAA ACAGGATCGA GACTTTGCCC CCGGAGCTGT TCAAGTGCAA GAAGCTGCGC 
ACTCTGAATC TGGGAAACAA CTGCCTGCAG ACGCTGCCGT CGCGCTTCGG AGAGCTCACC 
GGGTTGACGC AGCTGGAGCT GAGAGGGAAC CGTCTCGAGT GTCTCCCCGT GGAGCTCGGC 
GAGTGTCGCC TGCTGAAGAG GAGCGGCCTG GTGGTGGAGG AGGACCTGTT CAACACGCTG 
CCGTCGGAAG TCAAAGAGCA GCTCTGGAGG GCCGATAAGG AGCAGGCTTG A