spire1 gene cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus)

mRNA	Protein	Name
XM_017413143.1	XP_017268632.1	protein spire homolog 1 isoform X1
XM_017413146.1	XP_017268635.1	protein spire homolog 1 isoform X4
XM_017413145.1	XP_017268634.1	protein spire homolog 1 isoform X3
XM_017413144.2	XP_017268633.1	protein spire homolog 1 isoform X2
XM_017413145.2	XP_017268634.1	protein spire homolog 1 isoform X3
XM_017413143.2	XP_017268632.1	protein spire homolog 1 isoform X1
XM_017413146.2	XP_017268635.1	protein spire homolog 1 isoform X4
XM_017413147.2	XP_017268636.1	protein spire homolog 1 isoform X5
XM_017413144.1	XP_017268633.1	protein spire homolog 1 isoform X2
XM_017413147.1	XP_017268636.1	protein spire homolog 1 isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OKr09723	XM_017413144.2 Latest version!	Kryptolebias marmoratus spire type actin nucleation factor 1 (spire1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OKr09723	XM_017413143.2 Latest version!	Kryptolebias marmoratus spire type actin nucleation factor 1 (spire1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OKr09724	XM_017413145.2 Latest version!	Kryptolebias marmoratus spire type actin nucleation factor 1 (spire1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OKr09725	XM_017413146.2 Latest version!	Kryptolebias marmoratus spire type actin nucleation factor 1 (spire1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OKr09726	XM_017413147.2 Latest version!	Kryptolebias marmoratus spire type actin nucleation factor 1 (spire1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
View Old Accession Versions >>
OKr09724	XM_017413145.1	Kryptolebias marmoratus spire type actin nucleation factor 1 (spire1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OKr09723	XM_017413143.1	Kryptolebias marmoratus spire type actin nucleation factor 1 (spire1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OKr09725	XM_017413146.1	Kryptolebias marmoratus spire type actin nucleation factor 1 (spire1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OKr09723	XM_017413144.1	Kryptolebias marmoratus spire type actin nucleation factor 1 (spire1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OKr09726	XM_017413147.1	Kryptolebias marmoratus spire type actin nucleation factor 1 (spire1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
Hide Old Accession Versions >>

spire1 ( XM_017413143.1 ) cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus) -> XP_017268632.1 Kryptolebias marmoratus spire type actin nucleation factor 1 (spire1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OKr09723
Clone ID Related Accession (Same CDS sequence)	XM_017413143.1 , XM_017413144.2 , XM_017413143.2 , XM_017413144.1
Accession Version	XM_017413143.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-27
Organism	Kryptolebias marmoratus(mangrove rivulus)
Product	protein spire homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016094264.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Kryptolebias marmoratus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGG CTGCGGCACC GACTCTCCGG GATGAGGAGC GGCACCGCGA CGCGATGAGT 
CCGCCGAAGG AGGAGGCCGG GGCGGAGGAC CTGTCCCTGG AGGAGATCCT GAAGCTCTAC 
AGCCAGCCCA TCAACGAGGA GCAGGCATGG GCGGTGTGCT ACCAGTGCTG CCGGACGCTG 
GCGGAGGCGC ACCGGGGCAG CAGGAGCTCC GCCGCCGCCG GAGCGTCCCC GGCTCCGGCG 
GCGGCTCCGG GGAAGATATC GGGACCCGGG GATGTTAGGA TAGGGAAGGA CGGCTCTGTG 
AGGGTGGATC ACCACGGCTG TGGAGGTAAA TACAGTCCCT GCACAACAAC AGAGGTCATC 
GAGTCTCTGG GCATCATGAT CTACAAAGCT CTGGACTATG GCCTGAAGGA GAACGAGGAG 
CGGGAACTGA GTCCTCCTCT GGAGCGCCTC ATTGATCTCA TGACAAATAT GGCCGAGGCA 
GAAAAGGACG CTTGCCCCGA TGAAGGTTAT GAGGCCACAG AGGAAGAGGA GGAGGCAGAG 
GATGACCCTG ATAATATCTC CGGAGTTCAC AGCTACAGAG ATATCCTCAA GCTTTGCACT 
GTTCACCTAC CCAGTCGATC AGATGCTCCG AGTCACTACC AGGCAGTTTG TCGAGCGCTT 
TATACGGAAA CCAGAGAGCT TCGGACGTTC CTGGAGAAGA TCAAGAGTGC CAAAGAGAAC 
CTTCGTAAGA TGGAGGAGGA GTCATCCGAG GAGCCTGTCA GAGACCTGAA TGAGCTGCAG 
AATGCTGACT GGGCTCGATT CTGGGTTCAA GTGATGAGGG ACCTCCGAGA AGGAGTCAAA 
CTGAAGAAGG TCCAGGAGCG TCAGTACAAC CCTCTGCCCA TCGAGTACCA GCTGACTCCG 
TATGAGATGC TCATGGACGA CATCAGGTCC AAACGCTACA AACTGCGCAA AGTCATGGTC 
AATGGAGACA TCCCACCGAG GTTAAAGAAA AGTGCTCATG AAGTCATCCT GGAGTTCATC 
AGGTCCAGAC CGCCTCTCAA TCCTGTGTCA GCTCGTAAGC TTAAGCCTCA GACTCAGCCT 
CCTCCGACTC TGCACGAGCG GATCCTGGAG GAGATCAAGG CTGAAAGGAA ACTTCGCCCG 
GTGTCACCTG ACATGATGCG CAGGGGAAGG CTGGTAATTC GGCCACTTAG CATGTCTTTC 
AGTTTTGATG CGTCAGGTTT TGGCAAAACT CGCAGTATGC CACAGGATTT GTTCCGTACA 
GGAATAGACG AGTTCAGTCC ATACATGGGC CGGAGGACGT CCAGCACTCT GTCTCTGAGT 
AACGGATCAG CCTCTCCCAG AGTAGAGCCC TCCGGTTCTC AGTCTGTACC TCAGAGGAAA 
AAACTCCTGA AGGCTCCCAG CCTCGCCGAG CTGGACAGCT CCGACTCAGA TGATGAGCTG 
ATTGGGCGAA CATCAGCGAG CAGCTCCAGT GTGGCGACAT CTCTGATGGA TGATACATCC 
CCAGAGTCTG TGCTGGGGAA GAAAACTCCA CCCATGTTCC TGCCCATCTC CTCCACACCT 
CAGCCAGAGA GGCGGCAGAC CCCCCAGAGG AGACACTCCA TCGAGAAGGA AACACCCACC 
ACCGTGCGGC TGTTTCAGCC ACCTTCCAAA CAAAGCTCCA AGTCTCTGGA GGAGTTCTGT 
TTCCCTGTGG AGAGCCTGTC CCTGACAGTG GAGGAGGTGA TGCACATCAG ACAGGTGCTG 
GTGAAAGCAG AGCTGGAGAA GTTTCAGCAG TACAAAGACA TCTACAACGC CATGAAGAAG 
GGAAAGCTTT GCTTCAGTTG TCGAACCAAA AGGTTTTCCC TTTTCACCTG GTCCTACACC 
TGCCAGTTCT GCAAAAAGCC TGTGTGCTCA CAGTGCTGTA AAAAGATGCG GCTACCATCC 
AAACCTTACT CCAGTCTGCC CATTTACTCC ATTGGCTCGA CCAACACTCT CCCCCGAGAC 
AGAGTAAATG CCATGGCTTC TGTAGGACAA GGACTCTCTG TGGCTGGGGG TCCGGGGCCG 
TCAGTGACGA CGGGAGGGGC TGCTGCATCC CCCGTGTTGA AAGGAGCTAC AAAGGCAGCA 
TCTAAAGGAG CTGGAAAAGC TACAGGAGCC GCAGCAGCTG CCAAAACTGA CAAATCCTCC 
GGGAGCCTGC AGCGTCACAG CATTCAAAAG ACCATGTCCA AGTTATCAAA GCATGGCTCA 
TTAAAGTCTC ACGAAGAGCT GGAGCTGCCC CCGGAGCTGA CGGAGGACTG GGCCACCATG 
GAGGTGTGTG TGGACTGCAA GAAGTTCATC TCCGACATAA TCGCCTCCAG CAAGCACAGT 
CTGTCACTGG CCACAAAGAG GGCTCGCTTA AAACGCAAGA CGCAGTCGTT TTACGTGACG 
TCCCCCAAAG GAGCGGAGGG CTACAGGCCC TCGGAGCGAA CGATCAACGA AATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017268632.1
CDS	128..2584
Translation	MTEAAAPTLRDEERHRDAMSPPKEEAGAEDLSLEEILKLYSQPINEEQAWAVCYQCCRTLAEAHRGSRSSAAAGASPAPAAAPGKISGPGDVRIGKDGSVRVDHHGCGGKYSPCTTTEVIESLGIMIYKALDYGLKENEERELSPPLERLIDLMTNMAEAEKDACPDEGYEATEEEEEAEDDPDNISGVHSYRDILKLCTVHLPSRSDAPSHYQAVCRALYTETRELRTFLEKIKSAKENLRKMEEESSEEPVRDLNELQNADWARFWVQVMRDLREGVKLKKVQERQYNPLPIEYQLTPYEMLMDDIRSKRYKLRKVMVNGDIPPRLKKSAHEVILEFIRSRPPLNPVSARKLKPQTQPPPTLHERILEEIKAERKLRPVSPDMMRRGRLVIRPLSMSFSFDASGFGKTRSMPQDLFRTGIDEFSPYMGRRTSSTLSLSNGSASPRVEPSGSQSVPQRKKLLKAPSLAELDSSDSDDELIGRTSASSSSVATSLMDDTSPESVLGKKTPPMFLPISSTPQPERRQTPQRRHSIEKETPTTVRLFQPPSKQSSKSLEEFCFPVESLSLTVEEVMHIRQVLVKAELEKFQQYKDIYNAMKKGKLCFSCRTKRFSLFTWSYTCQFCKKPVCSQCCKKMRLPSKPYSSLPIYSIGSTNTLPRDRVNAMASVGQGLSVAGGPGPSVTTGGAAASPVLKGATKAASKGAGKATGAAAAAKTDKSSGSLQRHSIQKTMSKLSKHGSLKSHEELELPPELTEDWATMEVCVDCKKFISDIIASSKHSLSLATKRARLKRKTQSFYVTSPKGAEGYRPSERTINEI

Target ORF information:

RefSeq Version	XM_017413143.1
Organism	Kryptolebias marmoratus(mangrove rivulus)
Definition	Kryptolebias marmoratus spire type actin nucleation factor 1 (spire1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017413143.1

ORF Insert Sequence:

ATGACGGAGG CTGCGGCACC GACTCTCCGG GATGAGGAGC GGCACCGCGA CGCGATGAGT 
CCGCCGAAGG AGGAGGCCGG GGCGGAGGAC CTGTCCCTGG AGGAGATCCT GAAGCTCTAC 
AGCCAGCCCA TCAACGAGGA GCAGGCATGG GCGGTGTGCT ACCAGTGCTG CCGGACGCTG 
GCGGAGGCGC ACCGGGGCAG CAGGAGCTCC GCCGCCGCCG GAGCGTCCCC GGCTCCGGCG 
GCGGCTCCGG GGAAGATATC GGGACCCGGG GATGTTAGGA TAGGGAAGGA CGGCTCTGTG 
AGGGTGGATC ACCACGGCTG TGGAGGTAAA TACAGTCCCT GCACAACAAC AGAGGTCATC 
GAGTCTCTGG GCATCATGAT CTACAAAGCT CTGGACTATG GCCTGAAGGA GAACGAGGAG 
CGGGAACTGA GTCCTCCTCT GGAGCGCCTC ATTGATCTCA TGACAAATAT GGCCGAGGCA 
GAAAAGGACG CTTGCCCCGA TGAAGGTTAT GAGGCCACAG AGGAAGAGGA GGAGGCAGAG 
GATGACCCTG ATAATATCTC CGGAGTTCAC AGCTACAGAG ATATCCTCAA GCTTTGCACT 
GTTCACCTAC CCAGTCGATC AGATGCTCCG AGTCACTACC AGGCAGTTTG TCGAGCGCTT 
TATACGGAAA CCAGAGAGCT TCGGACGTTC CTGGAGAAGA TCAAGAGTGC CAAAGAGAAC 
CTTCGTAAGA TGGAGGAGGA GTCATCCGAG GAGCCTGTCA GAGACCTGAA TGAGCTGCAG 
AATGCTGACT GGGCTCGATT CTGGGTTCAA GTGATGAGGG ACCTCCGAGA AGGAGTCAAA 
CTGAAGAAGG TCCAGGAGCG TCAGTACAAC CCTCTGCCCA TCGAGTACCA GCTGACTCCG 
TATGAGATGC TCATGGACGA CATCAGGTCC AAACGCTACA AACTGCGCAA AGTCATGGTC 
AATGGAGACA TCCCACCGAG GTTAAAGAAA AGTGCTCATG AAGTCATCCT GGAGTTCATC 
AGGTCCAGAC CGCCTCTCAA TCCTGTGTCA GCTCGTAAGC TTAAGCCTCA GACTCAGCCT 
CCTCCGACTC TGCACGAGCG GATCCTGGAG GAGATCAAGG CTGAAAGGAA ACTTCGCCCG 
GTGTCACCTG ACATGATGCG CAGGGGAAGG CTGGTAATTC GGCCACTTAG CATGTCTTTC 
AGTTTTGATG CGTCAGGTTT TGGCAAAACT CGCAGTATGC CACAGGATTT GTTCCGTACA 
GGAATAGACG AGTTCAGTCC ATACATGGGC CGGAGGACGT CCAGCACTCT GTCTCTGAGT 
AACGGATCAG CCTCTCCCAG AGTAGAGCCC TCCGGTTCTC AGTCTGTACC TCAGAGGAAA 
AAACTCCTGA AGGCTCCCAG CCTCGCCGAG CTGGACAGCT CCGACTCAGA TGATGAGCTG 
ATTGGGCGAA CATCAGCGAG CAGCTCCAGT GTGGCGACAT CTCTGATGGA TGATACATCC 
CCAGAGTCTG TGCTGGGGAA GAAAACTCCA CCCATGTTCC TGCCCATCTC CTCCACACCT 
CAGCCAGAGA GGCGGCAGAC CCCCCAGAGG AGACACTCCA TCGAGAAGGA AACACCCACC 
ACCGTGCGGC TGTTTCAGCC ACCTTCCAAA CAAAGCTCCA AGTCTCTGGA GGAGTTCTGT 
TTCCCTGTGG AGAGCCTGTC CCTGACAGTG GAGGAGGTGA TGCACATCAG ACAGGTGCTG 
GTGAAAGCAG AGCTGGAGAA GTTTCAGCAG TACAAAGACA TCTACAACGC CATGAAGAAG 
GGAAAGCTTT GCTTCAGTTG TCGAACCAAA AGGTTTTCCC TTTTCACCTG GTCCTACACC 
TGCCAGTTCT GCAAAAAGCC TGTGTGCTCA CAGTGCTGTA AAAAGATGCG GCTACCATCC 
AAACCTTACT CCAGTCTGCC CATTTACTCC ATTGGCTCGA CCAACACTCT CCCCCGAGAC 
AGAGTAAATG CCATGGCTTC TGTAGGACAA GGACTCTCTG TGGCTGGGGG TCCGGGGCCG 
TCAGTGACGA CGGGAGGGGC TGCTGCATCC CCCGTGTTGA AAGGAGCTAC AAAGGCAGCA 
TCTAAAGGAG CTGGAAAAGC TACAGGAGCC GCAGCAGCTG CCAAAACTGA CAAATCCTCC 
GGGAGCCTGC AGCGTCACAG CATTCAAAAG ACCATGTCCA AGTTATCAAA GCATGGCTCA 
TTAAAGTCTC ACGAAGAGCT GGAGCTGCCC CCGGAGCTGA CGGAGGACTG GGCCACCATG 
GAGGTGTGTG TGGACTGCAA GAAGTTCATC TCCGACATAA TCGCCTCCAG CAAGCACAGT 
CTGTCACTGG CCACAAAGAG GGCTCGCTTA AAACGCAAGA CGCAGTCGTT TTACGTGACG 
TCCCCCAAAG GAGCGGAGGG CTACAGGCCC TCGGAGCGAA CGATCAACGA AATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spire1 ( XM_017413144.2 ) cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus) -> XP_017268633.1 Kryptolebias marmoratus spire type actin nucleation factor 1 (spire1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OKr09723
Clone ID Related Accession (Same CDS sequence)	XM_017413143.1 , XM_017413144.2 , XM_017413143.2 , XM_017413144.1
Accession Version	XM_017413144.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-03
Organism	Kryptolebias marmoratus(mangrove rivulus)
Product	protein spire homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016094264.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 4, 2018 this sequence version replaced XM_017413144.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Kryptolebias marmoratus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGG CTGCGGCACC GACTCTCCGG GATGAGGAGC GGCACCGCGA CGCGATGAGT 
CCGCCGAAGG AGGAGGCCGG GGCGGAGGAC CTGTCCCTGG AGGAGATCCT GAAGCTCTAC 
AGCCAGCCCA TCAACGAGGA GCAGGCATGG GCGGTGTGCT ACCAGTGCTG CCGGACGCTG 
GCGGAGGCGC ACCGGGGCAG CAGGAGCTCC GCCGCCGCCG GAGCGTCCCC GGCTCCGGCG 
GCGGCTCCGG GGAAGATATC GGGACCCGGG GATGTTAGGA TAGGGAAGGA CGGCTCTGTG 
AGGGTGGATC ACCACGGCTG TGGAGGTAAA TACAGTCCCT GCACAACAAC AGAGGTCATC 
GAGTCTCTGG GCATCATGAT CTACAAAGCT CTGGACTATG GCCTGAAGGA GAACGAGGAG 
CGGGAACTGA GTCCTCCTCT GGAGCGCCTC ATTGATCTCA TGACAAATAT GGCCGAGGCA 
GAAAAGGACG CTTGCCCCGA TGAAGGTTAT GAGGCCACAG AGGAAGAGGA GGAGGCAGAG 
GATGACCCTG ATAATATCTC CGGAGTTCAC AGCTACAGAG ATATCCTCAA GCTTTGCACT 
GTTCACCTAC CCAGTCGATC AGATGCTCCG AGTCACTACC AGGCAGTTTG TCGAGCGCTT 
TATACGGAAA CCAGAGAGCT TCGGACGTTC CTGGAGAAGA TCAAGAGTGC CAAAGAGAAC 
CTTCGTAAGA TGGAGGAGGA GTCATCCGAG GAGCCTGTCA GAGACCTGAA TGAGCTGCAG 
AATGCTGACT GGGCTCGATT CTGGGTTCAA GTGATGAGGG ACCTCCGAGA AGGAGTCAAA 
CTGAAGAAGG TCCAGGAGCG TCAGTACAAC CCTCTGCCCA TCGAGTACCA GCTGACTCCG 
TATGAGATGC TCATGGACGA CATCAGGTCC AAACGCTACA AACTGCGCAA AGTCATGGTC 
AATGGAGACA TCCCACCGAG GTTAAAGAAA AGTGCTCATG AAGTCATCCT GGAGTTCATC 
AGGTCCAGAC CGCCTCTCAA TCCTGTGTCA GCTCGTAAGC TTAAGCCTCA GACTCAGCCT 
CCTCCGACTC TGCACGAGCG GATCCTGGAG GAGATCAAGG CTGAAAGGAA ACTTCGCCCG 
GTGTCACCTG ACATGATGCG CAGGGGAAGG CTGGTAATTC GGCCACTTAG CATGTCTTTC 
AGTTTTGATG CGTCAGGTTT TGGCAAAACT CGCAGTATGC CACAGGATTT GTTCCGTACA 
GGAATAGACG AGTTCAGTCC ATACATGGGC CGGAGGACGT CCAGCACTCT GTCTCTGAGT 
AACGGATCAG CCTCTCCCAG AGTAGAGCCC TCCGGTTCTC AGTCTGTACC TCAGAGGAAA 
AAACTCCTGA AGGCTCCCAG CCTCGCCGAG CTGGACAGCT CCGACTCAGA TGATGAGCTG 
ATTGGGCGAA CATCAGCGAG CAGCTCCAGT GTGGCGACAT CTCTGATGGA TGATACATCC 
CCAGAGTCTG TGCTGGGGAA GAAAACTCCA CCCATGTTCC TGCCCATCTC CTCCACACCT 
CAGCCAGAGA GGCGGCAGAC CCCCCAGAGG AGACACTCCA TCGAGAAGGA AACACCCACC 
ACCGTGCGGC TGTTTCAGCC ACCTTCCAAA CAAAGCTCCA AGTCTCTGGA GGAGTTCTGT 
TTCCCTGTGG AGAGCCTGTC CCTGACAGTG GAGGAGGTGA TGCACATCAG ACAGGTGCTG 
GTGAAAGCAG AGCTGGAGAA GTTTCAGCAG TACAAAGACA TCTACAACGC CATGAAGAAG 
GGAAAGCTTT GCTTCAGTTG TCGAACCAAA AGGTTTTCCC TTTTCACCTG GTCCTACACC 
TGCCAGTTCT GCAAAAAGCC TGTGTGCTCA CAGTGCTGTA AAAAGATGCG GCTACCATCC 
AAACCTTACT CCAGTCTGCC CATTTACTCC ATTGGCTCGA CCAACACTCT CCCCCGAGAC 
AGAGTAAATG CCATGGCTTC TGTAGGACAA GGACTCTCTG TGGCTGGGGG TCCGGGGCCG 
TCAGTGACGA CGGGAGGGGC TGCTGCATCC CCCGTGTTGA AAGGAGCTAC AAAGGCAGCA 
TCTAAAGGAG CTGGAAAAGC TACAGGAGCC GCAGCAGCTG CCAAAACTGA CAAATCCTCC 
GGGAGCCTGC AGCGTCACAG CATTCAAAAG ACCATGTCCA AGTTATCAAA GCATGGCTCA 
TTAAAGTCTC ACGAAGAGCT GGAGCTGCCC CCGGAGCTGA CGGAGGACTG GGCCACCATG 
GAGGTGTGTG TGGACTGCAA GAAGTTCATC TCCGACATAA TCGCCTCCAG CAAGCACAGT 
CTGTCACTGG CCACAAAGAG GGCTCGCTTA AAACGCAAGA CGCAGTCGTT TTACGTGACG 
TCCCCCAAAG GAGCGGAGGG CTACAGGCCC TCGGAGCGAA CGATCAACGA AATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017268633.1
CDS	174..2630
Translation	MTEAAAPTLRDEERHRDAMSPPKEEAGAEDLSLEEILKLYSQPINEEQAWAVCYQCCRTLAEAHRGSRSSAAAGASPAPAAAPGKISGPGDVRIGKDGSVRVDHHGCGGKYSPCTTTEVIESLGIMIYKALDYGLKENEERELSPPLERLIDLMTNMAEAEKDACPDEGYEATEEEEEAEDDPDNISGVHSYRDILKLCTVHLPSRSDAPSHYQAVCRALYTETRELRTFLEKIKSAKENLRKMEEESSEEPVRDLNELQNADWARFWVQVMRDLREGVKLKKVQERQYNPLPIEYQLTPYEMLMDDIRSKRYKLRKVMVNGDIPPRLKKSAHEVILEFIRSRPPLNPVSARKLKPQTQPPPTLHERILEEIKAERKLRPVSPDMMRRGRLVIRPLSMSFSFDASGFGKTRSMPQDLFRTGIDEFSPYMGRRTSSTLSLSNGSASPRVEPSGSQSVPQRKKLLKAPSLAELDSSDSDDELIGRTSASSSSVATSLMDDTSPESVLGKKTPPMFLPISSTPQPERRQTPQRRHSIEKETPTTVRLFQPPSKQSSKSLEEFCFPVESLSLTVEEVMHIRQVLVKAELEKFQQYKDIYNAMKKGKLCFSCRTKRFSLFTWSYTCQFCKKPVCSQCCKKMRLPSKPYSSLPIYSIGSTNTLPRDRVNAMASVGQGLSVAGGPGPSVTTGGAAASPVLKGATKAASKGAGKATGAAAAAKTDKSSGSLQRHSIQKTMSKLSKHGSLKSHEELELPPELTEDWATMEVCVDCKKFISDIIASSKHSLSLATKRARLKRKTQSFYVTSPKGAEGYRPSERTINEI

Target ORF information:

RefSeq Version	XM_017413144.2
Organism	Kryptolebias marmoratus(mangrove rivulus)
Definition	Kryptolebias marmoratus spire type actin nucleation factor 1 (spire1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017413144.2

ORF Insert Sequence:

ATGACGGAGG CTGCGGCACC GACTCTCCGG GATGAGGAGC GGCACCGCGA CGCGATGAGT 
CCGCCGAAGG AGGAGGCCGG GGCGGAGGAC CTGTCCCTGG AGGAGATCCT GAAGCTCTAC 
AGCCAGCCCA TCAACGAGGA GCAGGCATGG GCGGTGTGCT ACCAGTGCTG CCGGACGCTG 
GCGGAGGCGC ACCGGGGCAG CAGGAGCTCC GCCGCCGCCG GAGCGTCCCC GGCTCCGGCG 
GCGGCTCCGG GGAAGATATC GGGACCCGGG GATGTTAGGA TAGGGAAGGA CGGCTCTGTG 
AGGGTGGATC ACCACGGCTG TGGAGGTAAA TACAGTCCCT GCACAACAAC AGAGGTCATC 
GAGTCTCTGG GCATCATGAT CTACAAAGCT CTGGACTATG GCCTGAAGGA GAACGAGGAG 
CGGGAACTGA GTCCTCCTCT GGAGCGCCTC ATTGATCTCA TGACAAATAT GGCCGAGGCA 
GAAAAGGACG CTTGCCCCGA TGAAGGTTAT GAGGCCACAG AGGAAGAGGA GGAGGCAGAG 
GATGACCCTG ATAATATCTC CGGAGTTCAC AGCTACAGAG ATATCCTCAA GCTTTGCACT 
GTTCACCTAC CCAGTCGATC AGATGCTCCG AGTCACTACC AGGCAGTTTG TCGAGCGCTT 
TATACGGAAA CCAGAGAGCT TCGGACGTTC CTGGAGAAGA TCAAGAGTGC CAAAGAGAAC 
CTTCGTAAGA TGGAGGAGGA GTCATCCGAG GAGCCTGTCA GAGACCTGAA TGAGCTGCAG 
AATGCTGACT GGGCTCGATT CTGGGTTCAA GTGATGAGGG ACCTCCGAGA AGGAGTCAAA 
CTGAAGAAGG TCCAGGAGCG TCAGTACAAC CCTCTGCCCA TCGAGTACCA GCTGACTCCG 
TATGAGATGC TCATGGACGA CATCAGGTCC AAACGCTACA AACTGCGCAA AGTCATGGTC 
AATGGAGACA TCCCACCGAG GTTAAAGAAA AGTGCTCATG AAGTCATCCT GGAGTTCATC 
AGGTCCAGAC CGCCTCTCAA TCCTGTGTCA GCTCGTAAGC TTAAGCCTCA GACTCAGCCT 
CCTCCGACTC TGCACGAGCG GATCCTGGAG GAGATCAAGG CTGAAAGGAA ACTTCGCCCG 
GTGTCACCTG ACATGATGCG CAGGGGAAGG CTGGTAATTC GGCCACTTAG CATGTCTTTC 
AGTTTTGATG CGTCAGGTTT TGGCAAAACT CGCAGTATGC CACAGGATTT GTTCCGTACA 
GGAATAGACG AGTTCAGTCC ATACATGGGC CGGAGGACGT CCAGCACTCT GTCTCTGAGT 
AACGGATCAG CCTCTCCCAG AGTAGAGCCC TCCGGTTCTC AGTCTGTACC TCAGAGGAAA 
AAACTCCTGA AGGCTCCCAG CCTCGCCGAG CTGGACAGCT CCGACTCAGA TGATGAGCTG 
ATTGGGCGAA CATCAGCGAG CAGCTCCAGT GTGGCGACAT CTCTGATGGA TGATACATCC 
CCAGAGTCTG TGCTGGGGAA GAAAACTCCA CCCATGTTCC TGCCCATCTC CTCCACACCT 
CAGCCAGAGA GGCGGCAGAC CCCCCAGAGG AGACACTCCA TCGAGAAGGA AACACCCACC 
ACCGTGCGGC TGTTTCAGCC ACCTTCCAAA CAAAGCTCCA AGTCTCTGGA GGAGTTCTGT 
TTCCCTGTGG AGAGCCTGTC CCTGACAGTG GAGGAGGTGA TGCACATCAG ACAGGTGCTG 
GTGAAAGCAG AGCTGGAGAA GTTTCAGCAG TACAAAGACA TCTACAACGC CATGAAGAAG 
GGAAAGCTTT GCTTCAGTTG TCGAACCAAA AGGTTTTCCC TTTTCACCTG GTCCTACACC 
TGCCAGTTCT GCAAAAAGCC TGTGTGCTCA CAGTGCTGTA AAAAGATGCG GCTACCATCC 
AAACCTTACT CCAGTCTGCC CATTTACTCC ATTGGCTCGA CCAACACTCT CCCCCGAGAC 
AGAGTAAATG CCATGGCTTC TGTAGGACAA GGACTCTCTG TGGCTGGGGG TCCGGGGCCG 
TCAGTGACGA CGGGAGGGGC TGCTGCATCC CCCGTGTTGA AAGGAGCTAC AAAGGCAGCA 
TCTAAAGGAG CTGGAAAAGC TACAGGAGCC GCAGCAGCTG CCAAAACTGA CAAATCCTCC 
GGGAGCCTGC AGCGTCACAG CATTCAAAAG ACCATGTCCA AGTTATCAAA GCATGGCTCA 
TTAAAGTCTC ACGAAGAGCT GGAGCTGCCC CCGGAGCTGA CGGAGGACTG GGCCACCATG 
GAGGTGTGTG TGGACTGCAA GAAGTTCATC TCCGACATAA TCGCCTCCAG CAAGCACAGT 
CTGTCACTGG CCACAAAGAG GGCTCGCTTA AAACGCAAGA CGCAGTCGTT TTACGTGACG 
TCCCCCAAAG GAGCGGAGGG CTACAGGCCC TCGGAGCGAA CGATCAACGA AATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spire1 ( XM_017413143.2 ) cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus) -> XP_017268632.1 Kryptolebias marmoratus spire type actin nucleation factor 1 (spire1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OKr09723
Clone ID Related Accession (Same CDS sequence)	XM_017413143.1 , XM_017413144.2 , XM_017413143.2 , XM_017413144.1
Accession Version	XM_017413143.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-03
Organism	Kryptolebias marmoratus(mangrove rivulus)
Product	protein spire homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016094264.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 4, 2018 this sequence version replaced XM_017413143.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Kryptolebias marmoratus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGG CTGCGGCACC GACTCTCCGG GATGAGGAGC GGCACCGCGA CGCGATGAGT 
CCGCCGAAGG AGGAGGCCGG GGCGGAGGAC CTGTCCCTGG AGGAGATCCT GAAGCTCTAC 
AGCCAGCCCA TCAACGAGGA GCAGGCATGG GCGGTGTGCT ACCAGTGCTG CCGGACGCTG 
GCGGAGGCGC ACCGGGGCAG CAGGAGCTCC GCCGCCGCCG GAGCGTCCCC GGCTCCGGCG 
GCGGCTCCGG GGAAGATATC GGGACCCGGG GATGTTAGGA TAGGGAAGGA CGGCTCTGTG 
AGGGTGGATC ACCACGGCTG TGGAGGTAAA TACAGTCCCT GCACAACAAC AGAGGTCATC 
GAGTCTCTGG GCATCATGAT CTACAAAGCT CTGGACTATG GCCTGAAGGA GAACGAGGAG 
CGGGAACTGA GTCCTCCTCT GGAGCGCCTC ATTGATCTCA TGACAAATAT GGCCGAGGCA 
GAAAAGGACG CTTGCCCCGA TGAAGGTTAT GAGGCCACAG AGGAAGAGGA GGAGGCAGAG 
GATGACCCTG ATAATATCTC CGGAGTTCAC AGCTACAGAG ATATCCTCAA GCTTTGCACT 
GTTCACCTAC CCAGTCGATC AGATGCTCCG AGTCACTACC AGGCAGTTTG TCGAGCGCTT 
TATACGGAAA CCAGAGAGCT TCGGACGTTC CTGGAGAAGA TCAAGAGTGC CAAAGAGAAC 
CTTCGTAAGA TGGAGGAGGA GTCATCCGAG GAGCCTGTCA GAGACCTGAA TGAGCTGCAG 
AATGCTGACT GGGCTCGATT CTGGGTTCAA GTGATGAGGG ACCTCCGAGA AGGAGTCAAA 
CTGAAGAAGG TCCAGGAGCG TCAGTACAAC CCTCTGCCCA TCGAGTACCA GCTGACTCCG 
TATGAGATGC TCATGGACGA CATCAGGTCC AAACGCTACA AACTGCGCAA AGTCATGGTC 
AATGGAGACA TCCCACCGAG GTTAAAGAAA AGTGCTCATG AAGTCATCCT GGAGTTCATC 
AGGTCCAGAC CGCCTCTCAA TCCTGTGTCA GCTCGTAAGC TTAAGCCTCA GACTCAGCCT 
CCTCCGACTC TGCACGAGCG GATCCTGGAG GAGATCAAGG CTGAAAGGAA ACTTCGCCCG 
GTGTCACCTG ACATGATGCG CAGGGGAAGG CTGGTAATTC GGCCACTTAG CATGTCTTTC 
AGTTTTGATG CGTCAGGTTT TGGCAAAACT CGCAGTATGC CACAGGATTT GTTCCGTACA 
GGAATAGACG AGTTCAGTCC ATACATGGGC CGGAGGACGT CCAGCACTCT GTCTCTGAGT 
AACGGATCAG CCTCTCCCAG AGTAGAGCCC TCCGGTTCTC AGTCTGTACC TCAGAGGAAA 
AAACTCCTGA AGGCTCCCAG CCTCGCCGAG CTGGACAGCT CCGACTCAGA TGATGAGCTG 
ATTGGGCGAA CATCAGCGAG CAGCTCCAGT GTGGCGACAT CTCTGATGGA TGATACATCC 
CCAGAGTCTG TGCTGGGGAA GAAAACTCCA CCCATGTTCC TGCCCATCTC CTCCACACCT 
CAGCCAGAGA GGCGGCAGAC CCCCCAGAGG AGACACTCCA TCGAGAAGGA AACACCCACC 
ACCGTGCGGC TGTTTCAGCC ACCTTCCAAA CAAAGCTCCA AGTCTCTGGA GGAGTTCTGT 
TTCCCTGTGG AGAGCCTGTC CCTGACAGTG GAGGAGGTGA TGCACATCAG ACAGGTGCTG 
GTGAAAGCAG AGCTGGAGAA GTTTCAGCAG TACAAAGACA TCTACAACGC CATGAAGAAG 
GGAAAGCTTT GCTTCAGTTG TCGAACCAAA AGGTTTTCCC TTTTCACCTG GTCCTACACC 
TGCCAGTTCT GCAAAAAGCC TGTGTGCTCA CAGTGCTGTA AAAAGATGCG GCTACCATCC 
AAACCTTACT CCAGTCTGCC CATTTACTCC ATTGGCTCGA CCAACACTCT CCCCCGAGAC 
AGAGTAAATG CCATGGCTTC TGTAGGACAA GGACTCTCTG TGGCTGGGGG TCCGGGGCCG 
TCAGTGACGA CGGGAGGGGC TGCTGCATCC CCCGTGTTGA AAGGAGCTAC AAAGGCAGCA 
TCTAAAGGAG CTGGAAAAGC TACAGGAGCC GCAGCAGCTG CCAAAACTGA CAAATCCTCC 
GGGAGCCTGC AGCGTCACAG CATTCAAAAG ACCATGTCCA AGTTATCAAA GCATGGCTCA 
TTAAAGTCTC ACGAAGAGCT GGAGCTGCCC CCGGAGCTGA CGGAGGACTG GGCCACCATG 
GAGGTGTGTG TGGACTGCAA GAAGTTCATC TCCGACATAA TCGCCTCCAG CAAGCACAGT 
CTGTCACTGG CCACAAAGAG GGCTCGCTTA AAACGCAAGA CGCAGTCGTT TTACGTGACG 
TCCCCCAAAG GAGCGGAGGG CTACAGGCCC TCGGAGCGAA CGATCAACGA AATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017268632.1
CDS	173..2629
Translation	MTEAAAPTLRDEERHRDAMSPPKEEAGAEDLSLEEILKLYSQPINEEQAWAVCYQCCRTLAEAHRGSRSSAAAGASPAPAAAPGKISGPGDVRIGKDGSVRVDHHGCGGKYSPCTTTEVIESLGIMIYKALDYGLKENEERELSPPLERLIDLMTNMAEAEKDACPDEGYEATEEEEEAEDDPDNISGVHSYRDILKLCTVHLPSRSDAPSHYQAVCRALYTETRELRTFLEKIKSAKENLRKMEEESSEEPVRDLNELQNADWARFWVQVMRDLREGVKLKKVQERQYNPLPIEYQLTPYEMLMDDIRSKRYKLRKVMVNGDIPPRLKKSAHEVILEFIRSRPPLNPVSARKLKPQTQPPPTLHERILEEIKAERKLRPVSPDMMRRGRLVIRPLSMSFSFDASGFGKTRSMPQDLFRTGIDEFSPYMGRRTSSTLSLSNGSASPRVEPSGSQSVPQRKKLLKAPSLAELDSSDSDDELIGRTSASSSSVATSLMDDTSPESVLGKKTPPMFLPISSTPQPERRQTPQRRHSIEKETPTTVRLFQPPSKQSSKSLEEFCFPVESLSLTVEEVMHIRQVLVKAELEKFQQYKDIYNAMKKGKLCFSCRTKRFSLFTWSYTCQFCKKPVCSQCCKKMRLPSKPYSSLPIYSIGSTNTLPRDRVNAMASVGQGLSVAGGPGPSVTTGGAAASPVLKGATKAASKGAGKATGAAAAAKTDKSSGSLQRHSIQKTMSKLSKHGSLKSHEELELPPELTEDWATMEVCVDCKKFISDIIASSKHSLSLATKRARLKRKTQSFYVTSPKGAEGYRPSERTINEI

Target ORF information:

RefSeq Version	XM_017413143.2
Organism	Kryptolebias marmoratus(mangrove rivulus)
Definition	Kryptolebias marmoratus spire type actin nucleation factor 1 (spire1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017413143.2

ORF Insert Sequence:

ATGACGGAGG CTGCGGCACC GACTCTCCGG GATGAGGAGC GGCACCGCGA CGCGATGAGT 
CCGCCGAAGG AGGAGGCCGG GGCGGAGGAC CTGTCCCTGG AGGAGATCCT GAAGCTCTAC 
AGCCAGCCCA TCAACGAGGA GCAGGCATGG GCGGTGTGCT ACCAGTGCTG CCGGACGCTG 
GCGGAGGCGC ACCGGGGCAG CAGGAGCTCC GCCGCCGCCG GAGCGTCCCC GGCTCCGGCG 
GCGGCTCCGG GGAAGATATC GGGACCCGGG GATGTTAGGA TAGGGAAGGA CGGCTCTGTG 
AGGGTGGATC ACCACGGCTG TGGAGGTAAA TACAGTCCCT GCACAACAAC AGAGGTCATC 
GAGTCTCTGG GCATCATGAT CTACAAAGCT CTGGACTATG GCCTGAAGGA GAACGAGGAG 
CGGGAACTGA GTCCTCCTCT GGAGCGCCTC ATTGATCTCA TGACAAATAT GGCCGAGGCA 
GAAAAGGACG CTTGCCCCGA TGAAGGTTAT GAGGCCACAG AGGAAGAGGA GGAGGCAGAG 
GATGACCCTG ATAATATCTC CGGAGTTCAC AGCTACAGAG ATATCCTCAA GCTTTGCACT 
GTTCACCTAC CCAGTCGATC AGATGCTCCG AGTCACTACC AGGCAGTTTG TCGAGCGCTT 
TATACGGAAA CCAGAGAGCT TCGGACGTTC CTGGAGAAGA TCAAGAGTGC CAAAGAGAAC 
CTTCGTAAGA TGGAGGAGGA GTCATCCGAG GAGCCTGTCA GAGACCTGAA TGAGCTGCAG 
AATGCTGACT GGGCTCGATT CTGGGTTCAA GTGATGAGGG ACCTCCGAGA AGGAGTCAAA 
CTGAAGAAGG TCCAGGAGCG TCAGTACAAC CCTCTGCCCA TCGAGTACCA GCTGACTCCG 
TATGAGATGC TCATGGACGA CATCAGGTCC AAACGCTACA AACTGCGCAA AGTCATGGTC 
AATGGAGACA TCCCACCGAG GTTAAAGAAA AGTGCTCATG AAGTCATCCT GGAGTTCATC 
AGGTCCAGAC CGCCTCTCAA TCCTGTGTCA GCTCGTAAGC TTAAGCCTCA GACTCAGCCT 
CCTCCGACTC TGCACGAGCG GATCCTGGAG GAGATCAAGG CTGAAAGGAA ACTTCGCCCG 
GTGTCACCTG ACATGATGCG CAGGGGAAGG CTGGTAATTC GGCCACTTAG CATGTCTTTC 
AGTTTTGATG CGTCAGGTTT TGGCAAAACT CGCAGTATGC CACAGGATTT GTTCCGTACA 
GGAATAGACG AGTTCAGTCC ATACATGGGC CGGAGGACGT CCAGCACTCT GTCTCTGAGT 
AACGGATCAG CCTCTCCCAG AGTAGAGCCC TCCGGTTCTC AGTCTGTACC TCAGAGGAAA 
AAACTCCTGA AGGCTCCCAG CCTCGCCGAG CTGGACAGCT CCGACTCAGA TGATGAGCTG 
ATTGGGCGAA CATCAGCGAG CAGCTCCAGT GTGGCGACAT CTCTGATGGA TGATACATCC 
CCAGAGTCTG TGCTGGGGAA GAAAACTCCA CCCATGTTCC TGCCCATCTC CTCCACACCT 
CAGCCAGAGA GGCGGCAGAC CCCCCAGAGG AGACACTCCA TCGAGAAGGA AACACCCACC 
ACCGTGCGGC TGTTTCAGCC ACCTTCCAAA CAAAGCTCCA AGTCTCTGGA GGAGTTCTGT 
TTCCCTGTGG AGAGCCTGTC CCTGACAGTG GAGGAGGTGA TGCACATCAG ACAGGTGCTG 
GTGAAAGCAG AGCTGGAGAA GTTTCAGCAG TACAAAGACA TCTACAACGC CATGAAGAAG 
GGAAAGCTTT GCTTCAGTTG TCGAACCAAA AGGTTTTCCC TTTTCACCTG GTCCTACACC 
TGCCAGTTCT GCAAAAAGCC TGTGTGCTCA CAGTGCTGTA AAAAGATGCG GCTACCATCC 
AAACCTTACT CCAGTCTGCC CATTTACTCC ATTGGCTCGA CCAACACTCT CCCCCGAGAC 
AGAGTAAATG CCATGGCTTC TGTAGGACAA GGACTCTCTG TGGCTGGGGG TCCGGGGCCG 
TCAGTGACGA CGGGAGGGGC TGCTGCATCC CCCGTGTTGA AAGGAGCTAC AAAGGCAGCA 
TCTAAAGGAG CTGGAAAAGC TACAGGAGCC GCAGCAGCTG CCAAAACTGA CAAATCCTCC 
GGGAGCCTGC AGCGTCACAG CATTCAAAAG ACCATGTCCA AGTTATCAAA GCATGGCTCA 
TTAAAGTCTC ACGAAGAGCT GGAGCTGCCC CCGGAGCTGA CGGAGGACTG GGCCACCATG 
GAGGTGTGTG TGGACTGCAA GAAGTTCATC TCCGACATAA TCGCCTCCAG CAAGCACAGT 
CTGTCACTGG CCACAAAGAG GGCTCGCTTA AAACGCAAGA CGCAGTCGTT TTACGTGACG 
TCCCCCAAAG GAGCGGAGGG CTACAGGCCC TCGGAGCGAA CGATCAACGA AATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spire1 ( XM_017413144.1 ) cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus) -> XP_017268633.1 Kryptolebias marmoratus spire type actin nucleation factor 1 (spire1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OKr09723
Clone ID Related Accession (Same CDS sequence)	XM_017413143.1 , XM_017413144.2 , XM_017413143.2 , XM_017413144.1
Accession Version	XM_017413144.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-27
Organism	Kryptolebias marmoratus(mangrove rivulus)
Product	protein spire homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016094264.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Kryptolebias marmoratus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGG CTGCGGCACC GACTCTCCGG GATGAGGAGC GGCACCGCGA CGCGATGAGT 
CCGCCGAAGG AGGAGGCCGG GGCGGAGGAC CTGTCCCTGG AGGAGATCCT GAAGCTCTAC 
AGCCAGCCCA TCAACGAGGA GCAGGCATGG GCGGTGTGCT ACCAGTGCTG CCGGACGCTG 
GCGGAGGCGC ACCGGGGCAG CAGGAGCTCC GCCGCCGCCG GAGCGTCCCC GGCTCCGGCG 
GCGGCTCCGG GGAAGATATC GGGACCCGGG GATGTTAGGA TAGGGAAGGA CGGCTCTGTG 
AGGGTGGATC ACCACGGCTG TGGAGGTAAA TACAGTCCCT GCACAACAAC AGAGGTCATC 
GAGTCTCTGG GCATCATGAT CTACAAAGCT CTGGACTATG GCCTGAAGGA GAACGAGGAG 
CGGGAACTGA GTCCTCCTCT GGAGCGCCTC ATTGATCTCA TGACAAATAT GGCCGAGGCA 
GAAAAGGACG CTTGCCCCGA TGAAGGTTAT GAGGCCACAG AGGAAGAGGA GGAGGCAGAG 
GATGACCCTG ATAATATCTC CGGAGTTCAC AGCTACAGAG ATATCCTCAA GCTTTGCACT 
GTTCACCTAC CCAGTCGATC AGATGCTCCG AGTCACTACC AGGCAGTTTG TCGAGCGCTT 
TATACGGAAA CCAGAGAGCT TCGGACGTTC CTGGAGAAGA TCAAGAGTGC CAAAGAGAAC 
CTTCGTAAGA TGGAGGAGGA GTCATCCGAG GAGCCTGTCA GAGACCTGAA TGAGCTGCAG 
AATGCTGACT GGGCTCGATT CTGGGTTCAA GTGATGAGGG ACCTCCGAGA AGGAGTCAAA 
CTGAAGAAGG TCCAGGAGCG TCAGTACAAC CCTCTGCCCA TCGAGTACCA GCTGACTCCG 
TATGAGATGC TCATGGACGA CATCAGGTCC AAACGCTACA AACTGCGCAA AGTCATGGTC 
AATGGAGACA TCCCACCGAG GTTAAAGAAA AGTGCTCATG AAGTCATCCT GGAGTTCATC 
AGGTCCAGAC CGCCTCTCAA TCCTGTGTCA GCTCGTAAGC TTAAGCCTCA GACTCAGCCT 
CCTCCGACTC TGCACGAGCG GATCCTGGAG GAGATCAAGG CTGAAAGGAA ACTTCGCCCG 
GTGTCACCTG ACATGATGCG CAGGGGAAGG CTGGTAATTC GGCCACTTAG CATGTCTTTC 
AGTTTTGATG CGTCAGGTTT TGGCAAAACT CGCAGTATGC CACAGGATTT GTTCCGTACA 
GGAATAGACG AGTTCAGTCC ATACATGGGC CGGAGGACGT CCAGCACTCT GTCTCTGAGT 
AACGGATCAG CCTCTCCCAG AGTAGAGCCC TCCGGTTCTC AGTCTGTACC TCAGAGGAAA 
AAACTCCTGA AGGCTCCCAG CCTCGCCGAG CTGGACAGCT CCGACTCAGA TGATGAGCTG 
ATTGGGCGAA CATCAGCGAG CAGCTCCAGT GTGGCGACAT CTCTGATGGA TGATACATCC 
CCAGAGTCTG TGCTGGGGAA GAAAACTCCA CCCATGTTCC TGCCCATCTC CTCCACACCT 
CAGCCAGAGA GGCGGCAGAC CCCCCAGAGG AGACACTCCA TCGAGAAGGA AACACCCACC 
ACCGTGCGGC TGTTTCAGCC ACCTTCCAAA CAAAGCTCCA AGTCTCTGGA GGAGTTCTGT 
TTCCCTGTGG AGAGCCTGTC CCTGACAGTG GAGGAGGTGA TGCACATCAG ACAGGTGCTG 
GTGAAAGCAG AGCTGGAGAA GTTTCAGCAG TACAAAGACA TCTACAACGC CATGAAGAAG 
GGAAAGCTTT GCTTCAGTTG TCGAACCAAA AGGTTTTCCC TTTTCACCTG GTCCTACACC 
TGCCAGTTCT GCAAAAAGCC TGTGTGCTCA CAGTGCTGTA AAAAGATGCG GCTACCATCC 
AAACCTTACT CCAGTCTGCC CATTTACTCC ATTGGCTCGA CCAACACTCT CCCCCGAGAC 
AGAGTAAATG CCATGGCTTC TGTAGGACAA GGACTCTCTG TGGCTGGGGG TCCGGGGCCG 
TCAGTGACGA CGGGAGGGGC TGCTGCATCC CCCGTGTTGA AAGGAGCTAC AAAGGCAGCA 
TCTAAAGGAG CTGGAAAAGC TACAGGAGCC GCAGCAGCTG CCAAAACTGA CAAATCCTCC 
GGGAGCCTGC AGCGTCACAG CATTCAAAAG ACCATGTCCA AGTTATCAAA GCATGGCTCA 
TTAAAGTCTC ACGAAGAGCT GGAGCTGCCC CCGGAGCTGA CGGAGGACTG GGCCACCATG 
GAGGTGTGTG TGGACTGCAA GAAGTTCATC TCCGACATAA TCGCCTCCAG CAAGCACAGT 
CTGTCACTGG CCACAAAGAG GGCTCGCTTA AAACGCAAGA CGCAGTCGTT TTACGTGACG 
TCCCCCAAAG GAGCGGAGGG CTACAGGCCC TCGGAGCGAA CGATCAACGA AATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017268633.1
CDS	128..2584
Translation	MTEAAAPTLRDEERHRDAMSPPKEEAGAEDLSLEEILKLYSQPINEEQAWAVCYQCCRTLAEAHRGSRSSAAAGASPAPAAAPGKISGPGDVRIGKDGSVRVDHHGCGGKYSPCTTTEVIESLGIMIYKALDYGLKENEERELSPPLERLIDLMTNMAEAEKDACPDEGYEATEEEEEAEDDPDNISGVHSYRDILKLCTVHLPSRSDAPSHYQAVCRALYTETRELRTFLEKIKSAKENLRKMEEESSEEPVRDLNELQNADWARFWVQVMRDLREGVKLKKVQERQYNPLPIEYQLTPYEMLMDDIRSKRYKLRKVMVNGDIPPRLKKSAHEVILEFIRSRPPLNPVSARKLKPQTQPPPTLHERILEEIKAERKLRPVSPDMMRRGRLVIRPLSMSFSFDASGFGKTRSMPQDLFRTGIDEFSPYMGRRTSSTLSLSNGSASPRVEPSGSQSVPQRKKLLKAPSLAELDSSDSDDELIGRTSASSSSVATSLMDDTSPESVLGKKTPPMFLPISSTPQPERRQTPQRRHSIEKETPTTVRLFQPPSKQSSKSLEEFCFPVESLSLTVEEVMHIRQVLVKAELEKFQQYKDIYNAMKKGKLCFSCRTKRFSLFTWSYTCQFCKKPVCSQCCKKMRLPSKPYSSLPIYSIGSTNTLPRDRVNAMASVGQGLSVAGGPGPSVTTGGAAASPVLKGATKAASKGAGKATGAAAAAKTDKSSGSLQRHSIQKTMSKLSKHGSLKSHEELELPPELTEDWATMEVCVDCKKFISDIIASSKHSLSLATKRARLKRKTQSFYVTSPKGAEGYRPSERTINEI

Target ORF information:

RefSeq Version	XM_017413144.1
Organism	Kryptolebias marmoratus(mangrove rivulus)
Definition	Kryptolebias marmoratus spire type actin nucleation factor 1 (spire1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017413144.1

ORF Insert Sequence:

ATGACGGAGG CTGCGGCACC GACTCTCCGG GATGAGGAGC GGCACCGCGA CGCGATGAGT 
CCGCCGAAGG AGGAGGCCGG GGCGGAGGAC CTGTCCCTGG AGGAGATCCT GAAGCTCTAC 
AGCCAGCCCA TCAACGAGGA GCAGGCATGG GCGGTGTGCT ACCAGTGCTG CCGGACGCTG 
GCGGAGGCGC ACCGGGGCAG CAGGAGCTCC GCCGCCGCCG GAGCGTCCCC GGCTCCGGCG 
GCGGCTCCGG GGAAGATATC GGGACCCGGG GATGTTAGGA TAGGGAAGGA CGGCTCTGTG 
AGGGTGGATC ACCACGGCTG TGGAGGTAAA TACAGTCCCT GCACAACAAC AGAGGTCATC 
GAGTCTCTGG GCATCATGAT CTACAAAGCT CTGGACTATG GCCTGAAGGA GAACGAGGAG 
CGGGAACTGA GTCCTCCTCT GGAGCGCCTC ATTGATCTCA TGACAAATAT GGCCGAGGCA 
GAAAAGGACG CTTGCCCCGA TGAAGGTTAT GAGGCCACAG AGGAAGAGGA GGAGGCAGAG 
GATGACCCTG ATAATATCTC CGGAGTTCAC AGCTACAGAG ATATCCTCAA GCTTTGCACT 
GTTCACCTAC CCAGTCGATC AGATGCTCCG AGTCACTACC AGGCAGTTTG TCGAGCGCTT 
TATACGGAAA CCAGAGAGCT TCGGACGTTC CTGGAGAAGA TCAAGAGTGC CAAAGAGAAC 
CTTCGTAAGA TGGAGGAGGA GTCATCCGAG GAGCCTGTCA GAGACCTGAA TGAGCTGCAG 
AATGCTGACT GGGCTCGATT CTGGGTTCAA GTGATGAGGG ACCTCCGAGA AGGAGTCAAA 
CTGAAGAAGG TCCAGGAGCG TCAGTACAAC CCTCTGCCCA TCGAGTACCA GCTGACTCCG 
TATGAGATGC TCATGGACGA CATCAGGTCC AAACGCTACA AACTGCGCAA AGTCATGGTC 
AATGGAGACA TCCCACCGAG GTTAAAGAAA AGTGCTCATG AAGTCATCCT GGAGTTCATC 
AGGTCCAGAC CGCCTCTCAA TCCTGTGTCA GCTCGTAAGC TTAAGCCTCA GACTCAGCCT 
CCTCCGACTC TGCACGAGCG GATCCTGGAG GAGATCAAGG CTGAAAGGAA ACTTCGCCCG 
GTGTCACCTG ACATGATGCG CAGGGGAAGG CTGGTAATTC GGCCACTTAG CATGTCTTTC 
AGTTTTGATG CGTCAGGTTT TGGCAAAACT CGCAGTATGC CACAGGATTT GTTCCGTACA 
GGAATAGACG AGTTCAGTCC ATACATGGGC CGGAGGACGT CCAGCACTCT GTCTCTGAGT 
AACGGATCAG CCTCTCCCAG AGTAGAGCCC TCCGGTTCTC AGTCTGTACC TCAGAGGAAA 
AAACTCCTGA AGGCTCCCAG CCTCGCCGAG CTGGACAGCT CCGACTCAGA TGATGAGCTG 
ATTGGGCGAA CATCAGCGAG CAGCTCCAGT GTGGCGACAT CTCTGATGGA TGATACATCC 
CCAGAGTCTG TGCTGGGGAA GAAAACTCCA CCCATGTTCC TGCCCATCTC CTCCACACCT 
CAGCCAGAGA GGCGGCAGAC CCCCCAGAGG AGACACTCCA TCGAGAAGGA AACACCCACC 
ACCGTGCGGC TGTTTCAGCC ACCTTCCAAA CAAAGCTCCA AGTCTCTGGA GGAGTTCTGT 
TTCCCTGTGG AGAGCCTGTC CCTGACAGTG GAGGAGGTGA TGCACATCAG ACAGGTGCTG 
GTGAAAGCAG AGCTGGAGAA GTTTCAGCAG TACAAAGACA TCTACAACGC CATGAAGAAG 
GGAAAGCTTT GCTTCAGTTG TCGAACCAAA AGGTTTTCCC TTTTCACCTG GTCCTACACC 
TGCCAGTTCT GCAAAAAGCC TGTGTGCTCA CAGTGCTGTA AAAAGATGCG GCTACCATCC 
AAACCTTACT CCAGTCTGCC CATTTACTCC ATTGGCTCGA CCAACACTCT CCCCCGAGAC 
AGAGTAAATG CCATGGCTTC TGTAGGACAA GGACTCTCTG TGGCTGGGGG TCCGGGGCCG 
TCAGTGACGA CGGGAGGGGC TGCTGCATCC CCCGTGTTGA AAGGAGCTAC AAAGGCAGCA 
TCTAAAGGAG CTGGAAAAGC TACAGGAGCC GCAGCAGCTG CCAAAACTGA CAAATCCTCC 
GGGAGCCTGC AGCGTCACAG CATTCAAAAG ACCATGTCCA AGTTATCAAA GCATGGCTCA 
TTAAAGTCTC ACGAAGAGCT GGAGCTGCCC CCGGAGCTGA CGGAGGACTG GGCCACCATG 
GAGGTGTGTG TGGACTGCAA GAAGTTCATC TCCGACATAA TCGCCTCCAG CAAGCACAGT 
CTGTCACTGG CCACAAAGAG GGCTCGCTTA AAACGCAAGA CGCAGTCGTT TTACGTGACG 
TCCCCCAAAG GAGCGGAGGG CTACAGGCCC TCGGAGCGAA CGATCAACGA AATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spire1 ( XM_017413145.1 ) cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus) -> XP_017268634.1 Kryptolebias marmoratus spire type actin nucleation factor 1 (spire1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OKr09724
Clone ID Related Accession (Same CDS sequence)	XM_017413145.1 , XM_017413145.2
Accession Version	XM_017413145.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2415bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-27
Organism	Kryptolebias marmoratus(mangrove rivulus)
Product	protein spire homolog 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016094264.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Kryptolebias marmoratus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGG CTGCGGCACC GACTCTCCGG GATGAGGAGC GGCACCGCGA CGCGATGAGT 
CCGCCGAAGG AGGAGGCCGG GGCGGAGGAC CTGTCCCTGG AGGAGATCCT GAAGCTCTAC 
AGCCAGCCCA TCAACGAGGA GCAGGCATGG GCGGTGTGCT ACCAGTGCTG CCGGACGCTG 
GCGGAGGCGC ACCGGGGCAG CAGGAGCTCC GCCGCCGCCG GAGCGTCCCC GGCTCCGGCG 
GCGGCTCCGG GGAAGATATC GGGACCCGGG GATGTTAGGA TAGGGAAGGA CGGCTCTGTG 
AGGGTGGATC ACCACGGCTG TGGAGGTAAA TACAGTCCCT GCACAACAAC AGAGGTCATC 
GAGTCTCTGG GCATCATGAT CTACAAAGCT CTGGACTATG GCCTGAAGGA GAACGAGGAG 
CGGGAACTGA GTCCTCCTCT GGAGCGCCTC ATTGATCTCA TGACAAATAT GGCCGAGGCA 
GAAAAGGACG CTTGCCCCGA TGAAGGTTAT GAGGCCACAG AGGAAGAGGA GGAGGCAGAG 
GATGACCCTG ATAATATCTC CGGAGTTCAC AGCTACAGAG ATATCCTCAA GCTTTGCACT 
GTTCACCTAC CCAGTCGATC AGATGCTCCG AGTCACTACC AGGCAGTTTG TCGAGCGCTT 
TATACGGAAA CCAGAGAGCT TCGGACGTTC CTGGAGAAGA TCAAGAGTGC CAAAGAGAAC 
CTTCGTAAGA TGGAGGAGGA GTCATCCGAG GAGCCTGTCA GAGACCTGAA TGAGCTGCAG 
AATGCTGACT GGGCTCGATT CTGGGTTCAA GTGATGAGGG ACCTCCGAGA AGGAGTCAAA 
CTGAAGAAGG TCCAGGAGCG TCAGTACAAC CCTCTGCCCA TCGAGTACCA GCTGACTCCG 
TATGAGATGC TCATGGACGA CATCAGGTCC AAACGCTACA AACTGCGCAA AGTCATGGTC 
AATGGAGACA TCCCACCGAG GTTAAAGAAA AGTGCTCATG AAGTCATCCT GGAGTTCATC 
AGGTCCAGAC CGCCTCTCAA TCCTGTGTCA GCTCGTAAGC TTAAGCCTCA GACTCAGCCT 
CCTCCGACTC TGCACGAGCG GATCCTGGAG GAGATCAAGG CTGAAAGGAA ACTTCGCCCG 
GTGTCACCTG ACATGATGCG CAGGGGAAGG CTGGGTTTTG GCAAAACTCG CAGTATGCCA 
CAGGATTTGT TCCGTACAGG AATAGACGAG TTCAGTCCAT ACATGGGCCG GAGGACGTCC 
AGCACTCTGT CTCTGAGTAA CGGATCAGCC TCTCCCAGAG TAGAGCCCTC CGGTTCTCAG 
TCTGTACCTC AGAGGAAAAA ACTCCTGAAG GCTCCCAGCC TCGCCGAGCT GGACAGCTCC 
GACTCAGATG ATGAGCTGAT TGGGCGAACA TCAGCGAGCA GCTCCAGTGT GGCGACATCT 
CTGATGGATG ATACATCCCC AGAGTCTGTG CTGGGGAAGA AAACTCCACC CATGTTCCTG 
CCCATCTCCT CCACACCTCA GCCAGAGAGG CGGCAGACCC CCCAGAGGAG ACACTCCATC 
GAGAAGGAAA CACCCACCAC CGTGCGGCTG TTTCAGCCAC CTTCCAAACA AAGCTCCAAG 
TCTCTGGAGG AGTTCTGTTT CCCTGTGGAG AGCCTGTCCC TGACAGTGGA GGAGGTGATG 
CACATCAGAC AGGTGCTGGT GAAAGCAGAG CTGGAGAAGT TTCAGCAGTA CAAAGACATC 
TACAACGCCA TGAAGAAGGG AAAGCTTTGC TTCAGTTGTC GAACCAAAAG GTTTTCCCTT 
TTCACCTGGT CCTACACCTG CCAGTTCTGC AAAAAGCCTG TGTGCTCACA GTGCTGTAAA 
AAGATGCGGC TACCATCCAA ACCTTACTCC AGTCTGCCCA TTTACTCCAT TGGCTCGACC 
AACACTCTCC CCCGAGACAG AGTAAATGCC ATGGCTTCTG TAGGACAAGG ACTCTCTGTG 
GCTGGGGGTC CGGGGCCGTC AGTGACGACG GGAGGGGCTG CTGCATCCCC CGTGTTGAAA 
GGAGCTACAA AGGCAGCATC TAAAGGAGCT GGAAAAGCTA CAGGAGCCGC AGCAGCTGCC 
AAAACTGACA AATCCTCCGG GAGCCTGCAG CGTCACAGCA TTCAAAAGAC CATGTCCAAG 
TTATCAAAGC ATGGCTCATT AAAGTCTCAC GAAGAGCTGG AGCTGCCCCC GGAGCTGACG 
GAGGACTGGG CCACCATGGA GGTGTGTGTG GACTGCAAGA AGTTCATCTC CGACATAATC 
GCCTCCAGCA AGCACAGTCT GTCACTGGCC ACAAAGAGGG CTCGCTTAAA ACGCAAGACG 
CAGTCGTTTT ACGTGACGTC CCCCAAAGGA GCGGAGGGCT ACAGGCCCTC GGAGCGAACG 
ATCAACGAAA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017268634.1
CDS	128..2542
Translation	MTEAAAPTLRDEERHRDAMSPPKEEAGAEDLSLEEILKLYSQPINEEQAWAVCYQCCRTLAEAHRGSRSSAAAGASPAPAAAPGKISGPGDVRIGKDGSVRVDHHGCGGKYSPCTTTEVIESLGIMIYKALDYGLKENEERELSPPLERLIDLMTNMAEAEKDACPDEGYEATEEEEEAEDDPDNISGVHSYRDILKLCTVHLPSRSDAPSHYQAVCRALYTETRELRTFLEKIKSAKENLRKMEEESSEEPVRDLNELQNADWARFWVQVMRDLREGVKLKKVQERQYNPLPIEYQLTPYEMLMDDIRSKRYKLRKVMVNGDIPPRLKKSAHEVILEFIRSRPPLNPVSARKLKPQTQPPPTLHERILEEIKAERKLRPVSPDMMRRGRLGFGKTRSMPQDLFRTGIDEFSPYMGRRTSSTLSLSNGSASPRVEPSGSQSVPQRKKLLKAPSLAELDSSDSDDELIGRTSASSSSVATSLMDDTSPESVLGKKTPPMFLPISSTPQPERRQTPQRRHSIEKETPTTVRLFQPPSKQSSKSLEEFCFPVESLSLTVEEVMHIRQVLVKAELEKFQQYKDIYNAMKKGKLCFSCRTKRFSLFTWSYTCQFCKKPVCSQCCKKMRLPSKPYSSLPIYSIGSTNTLPRDRVNAMASVGQGLSVAGGPGPSVTTGGAAASPVLKGATKAASKGAGKATGAAAAAKTDKSSGSLQRHSIQKTMSKLSKHGSLKSHEELELPPELTEDWATMEVCVDCKKFISDIIASSKHSLSLATKRARLKRKTQSFYVTSPKGAEGYRPSERTINEI

Target ORF information:

RefSeq Version	XM_017413145.1
Organism	Kryptolebias marmoratus(mangrove rivulus)
Definition	Kryptolebias marmoratus spire type actin nucleation factor 1 (spire1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017413145.1

ORF Insert Sequence:

ATGACGGAGG CTGCGGCACC GACTCTCCGG GATGAGGAGC GGCACCGCGA CGCGATGAGT 
CCGCCGAAGG AGGAGGCCGG GGCGGAGGAC CTGTCCCTGG AGGAGATCCT GAAGCTCTAC 
AGCCAGCCCA TCAACGAGGA GCAGGCATGG GCGGTGTGCT ACCAGTGCTG CCGGACGCTG 
GCGGAGGCGC ACCGGGGCAG CAGGAGCTCC GCCGCCGCCG GAGCGTCCCC GGCTCCGGCG 
GCGGCTCCGG GGAAGATATC GGGACCCGGG GATGTTAGGA TAGGGAAGGA CGGCTCTGTG 
AGGGTGGATC ACCACGGCTG TGGAGGTAAA TACAGTCCCT GCACAACAAC AGAGGTCATC 
GAGTCTCTGG GCATCATGAT CTACAAAGCT CTGGACTATG GCCTGAAGGA GAACGAGGAG 
CGGGAACTGA GTCCTCCTCT GGAGCGCCTC ATTGATCTCA TGACAAATAT GGCCGAGGCA 
GAAAAGGACG CTTGCCCCGA TGAAGGTTAT GAGGCCACAG AGGAAGAGGA GGAGGCAGAG 
GATGACCCTG ATAATATCTC CGGAGTTCAC AGCTACAGAG ATATCCTCAA GCTTTGCACT 
GTTCACCTAC CCAGTCGATC AGATGCTCCG AGTCACTACC AGGCAGTTTG TCGAGCGCTT 
TATACGGAAA CCAGAGAGCT TCGGACGTTC CTGGAGAAGA TCAAGAGTGC CAAAGAGAAC 
CTTCGTAAGA TGGAGGAGGA GTCATCCGAG GAGCCTGTCA GAGACCTGAA TGAGCTGCAG 
AATGCTGACT GGGCTCGATT CTGGGTTCAA GTGATGAGGG ACCTCCGAGA AGGAGTCAAA 
CTGAAGAAGG TCCAGGAGCG TCAGTACAAC CCTCTGCCCA TCGAGTACCA GCTGACTCCG 
TATGAGATGC TCATGGACGA CATCAGGTCC AAACGCTACA AACTGCGCAA AGTCATGGTC 
AATGGAGACA TCCCACCGAG GTTAAAGAAA AGTGCTCATG AAGTCATCCT GGAGTTCATC 
AGGTCCAGAC CGCCTCTCAA TCCTGTGTCA GCTCGTAAGC TTAAGCCTCA GACTCAGCCT 
CCTCCGACTC TGCACGAGCG GATCCTGGAG GAGATCAAGG CTGAAAGGAA ACTTCGCCCG 
GTGTCACCTG ACATGATGCG CAGGGGAAGG CTGGGTTTTG GCAAAACTCG CAGTATGCCA 
CAGGATTTGT TCCGTACAGG AATAGACGAG TTCAGTCCAT ACATGGGCCG GAGGACGTCC 
AGCACTCTGT CTCTGAGTAA CGGATCAGCC TCTCCCAGAG TAGAGCCCTC CGGTTCTCAG 
TCTGTACCTC AGAGGAAAAA ACTCCTGAAG GCTCCCAGCC TCGCCGAGCT GGACAGCTCC 
GACTCAGATG ATGAGCTGAT TGGGCGAACA TCAGCGAGCA GCTCCAGTGT GGCGACATCT 
CTGATGGATG ATACATCCCC AGAGTCTGTG CTGGGGAAGA AAACTCCACC CATGTTCCTG 
CCCATCTCCT CCACACCTCA GCCAGAGAGG CGGCAGACCC CCCAGAGGAG ACACTCCATC 
GAGAAGGAAA CACCCACCAC CGTGCGGCTG TTTCAGCCAC CTTCCAAACA AAGCTCCAAG 
TCTCTGGAGG AGTTCTGTTT CCCTGTGGAG AGCCTGTCCC TGACAGTGGA GGAGGTGATG 
CACATCAGAC AGGTGCTGGT GAAAGCAGAG CTGGAGAAGT TTCAGCAGTA CAAAGACATC 
TACAACGCCA TGAAGAAGGG AAAGCTTTGC TTCAGTTGTC GAACCAAAAG GTTTTCCCTT 
TTCACCTGGT CCTACACCTG CCAGTTCTGC AAAAAGCCTG TGTGCTCACA GTGCTGTAAA 
AAGATGCGGC TACCATCCAA ACCTTACTCC AGTCTGCCCA TTTACTCCAT TGGCTCGACC 
AACACTCTCC CCCGAGACAG AGTAAATGCC ATGGCTTCTG TAGGACAAGG ACTCTCTGTG 
GCTGGGGGTC CGGGGCCGTC AGTGACGACG GGAGGGGCTG CTGCATCCCC CGTGTTGAAA 
GGAGCTACAA AGGCAGCATC TAAAGGAGCT GGAAAAGCTA CAGGAGCCGC AGCAGCTGCC 
AAAACTGACA AATCCTCCGG GAGCCTGCAG CGTCACAGCA TTCAAAAGAC CATGTCCAAG 
TTATCAAAGC ATGGCTCATT AAAGTCTCAC GAAGAGCTGG AGCTGCCCCC GGAGCTGACG 
GAGGACTGGG CCACCATGGA GGTGTGTGTG GACTGCAAGA AGTTCATCTC CGACATAATC 
GCCTCCAGCA AGCACAGTCT GTCACTGGCC ACAAAGAGGG CTCGCTTAAA ACGCAAGACG 
CAGTCGTTTT ACGTGACGTC CCCCAAAGGA GCGGAGGGCT ACAGGCCCTC GGAGCGAACG 
ATCAACGAAA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spire1 ( XM_017413145.2 ) cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus) -> XP_017268634.1 Kryptolebias marmoratus spire type actin nucleation factor 1 (spire1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OKr09724
Clone ID Related Accession (Same CDS sequence)	XM_017413145.1 , XM_017413145.2
Accession Version	XM_017413145.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2415bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-03
Organism	Kryptolebias marmoratus(mangrove rivulus)
Product	protein spire homolog 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016094264.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 4, 2018 this sequence version replaced XM_017413145.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Kryptolebias marmoratus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGG CTGCGGCACC GACTCTCCGG GATGAGGAGC GGCACCGCGA CGCGATGAGT 
CCGCCGAAGG AGGAGGCCGG GGCGGAGGAC CTGTCCCTGG AGGAGATCCT GAAGCTCTAC 
AGCCAGCCCA TCAACGAGGA GCAGGCATGG GCGGTGTGCT ACCAGTGCTG CCGGACGCTG 
GCGGAGGCGC ACCGGGGCAG CAGGAGCTCC GCCGCCGCCG GAGCGTCCCC GGCTCCGGCG 
GCGGCTCCGG GGAAGATATC GGGACCCGGG GATGTTAGGA TAGGGAAGGA CGGCTCTGTG 
AGGGTGGATC ACCACGGCTG TGGAGGTAAA TACAGTCCCT GCACAACAAC AGAGGTCATC 
GAGTCTCTGG GCATCATGAT CTACAAAGCT CTGGACTATG GCCTGAAGGA GAACGAGGAG 
CGGGAACTGA GTCCTCCTCT GGAGCGCCTC ATTGATCTCA TGACAAATAT GGCCGAGGCA 
GAAAAGGACG CTTGCCCCGA TGAAGGTTAT GAGGCCACAG AGGAAGAGGA GGAGGCAGAG 
GATGACCCTG ATAATATCTC CGGAGTTCAC AGCTACAGAG ATATCCTCAA GCTTTGCACT 
GTTCACCTAC CCAGTCGATC AGATGCTCCG AGTCACTACC AGGCAGTTTG TCGAGCGCTT 
TATACGGAAA CCAGAGAGCT TCGGACGTTC CTGGAGAAGA TCAAGAGTGC CAAAGAGAAC 
CTTCGTAAGA TGGAGGAGGA GTCATCCGAG GAGCCTGTCA GAGACCTGAA TGAGCTGCAG 
AATGCTGACT GGGCTCGATT CTGGGTTCAA GTGATGAGGG ACCTCCGAGA AGGAGTCAAA 
CTGAAGAAGG TCCAGGAGCG TCAGTACAAC CCTCTGCCCA TCGAGTACCA GCTGACTCCG 
TATGAGATGC TCATGGACGA CATCAGGTCC AAACGCTACA AACTGCGCAA AGTCATGGTC 
AATGGAGACA TCCCACCGAG GTTAAAGAAA AGTGCTCATG AAGTCATCCT GGAGTTCATC 
AGGTCCAGAC CGCCTCTCAA TCCTGTGTCA GCTCGTAAGC TTAAGCCTCA GACTCAGCCT 
CCTCCGACTC TGCACGAGCG GATCCTGGAG GAGATCAAGG CTGAAAGGAA ACTTCGCCCG 
GTGTCACCTG ACATGATGCG CAGGGGAAGG CTGGGTTTTG GCAAAACTCG CAGTATGCCA 
CAGGATTTGT TCCGTACAGG AATAGACGAG TTCAGTCCAT ACATGGGCCG GAGGACGTCC 
AGCACTCTGT CTCTGAGTAA CGGATCAGCC TCTCCCAGAG TAGAGCCCTC CGGTTCTCAG 
TCTGTACCTC AGAGGAAAAA ACTCCTGAAG GCTCCCAGCC TCGCCGAGCT GGACAGCTCC 
GACTCAGATG ATGAGCTGAT TGGGCGAACA TCAGCGAGCA GCTCCAGTGT GGCGACATCT 
CTGATGGATG ATACATCCCC AGAGTCTGTG CTGGGGAAGA AAACTCCACC CATGTTCCTG 
CCCATCTCCT CCACACCTCA GCCAGAGAGG CGGCAGACCC CCCAGAGGAG ACACTCCATC 
GAGAAGGAAA CACCCACCAC CGTGCGGCTG TTTCAGCCAC CTTCCAAACA AAGCTCCAAG 
TCTCTGGAGG AGTTCTGTTT CCCTGTGGAG AGCCTGTCCC TGACAGTGGA GGAGGTGATG 
CACATCAGAC AGGTGCTGGT GAAAGCAGAG CTGGAGAAGT TTCAGCAGTA CAAAGACATC 
TACAACGCCA TGAAGAAGGG AAAGCTTTGC TTCAGTTGTC GAACCAAAAG GTTTTCCCTT 
TTCACCTGGT CCTACACCTG CCAGTTCTGC AAAAAGCCTG TGTGCTCACA GTGCTGTAAA 
AAGATGCGGC TACCATCCAA ACCTTACTCC AGTCTGCCCA TTTACTCCAT TGGCTCGACC 
AACACTCTCC CCCGAGACAG AGTAAATGCC ATGGCTTCTG TAGGACAAGG ACTCTCTGTG 
GCTGGGGGTC CGGGGCCGTC AGTGACGACG GGAGGGGCTG CTGCATCCCC CGTGTTGAAA 
GGAGCTACAA AGGCAGCATC TAAAGGAGCT GGAAAAGCTA CAGGAGCCGC AGCAGCTGCC 
AAAACTGACA AATCCTCCGG GAGCCTGCAG CGTCACAGCA TTCAAAAGAC CATGTCCAAG 
TTATCAAAGC ATGGCTCATT AAAGTCTCAC GAAGAGCTGG AGCTGCCCCC GGAGCTGACG 
GAGGACTGGG CCACCATGGA GGTGTGTGTG GACTGCAAGA AGTTCATCTC CGACATAATC 
GCCTCCAGCA AGCACAGTCT GTCACTGGCC ACAAAGAGGG CTCGCTTAAA ACGCAAGACG 
CAGTCGTTTT ACGTGACGTC CCCCAAAGGA GCGGAGGGCT ACAGGCCCTC GGAGCGAACG 
ATCAACGAAA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017268634.1
CDS	173..2587
Translation	MTEAAAPTLRDEERHRDAMSPPKEEAGAEDLSLEEILKLYSQPINEEQAWAVCYQCCRTLAEAHRGSRSSAAAGASPAPAAAPGKISGPGDVRIGKDGSVRVDHHGCGGKYSPCTTTEVIESLGIMIYKALDYGLKENEERELSPPLERLIDLMTNMAEAEKDACPDEGYEATEEEEEAEDDPDNISGVHSYRDILKLCTVHLPSRSDAPSHYQAVCRALYTETRELRTFLEKIKSAKENLRKMEEESSEEPVRDLNELQNADWARFWVQVMRDLREGVKLKKVQERQYNPLPIEYQLTPYEMLMDDIRSKRYKLRKVMVNGDIPPRLKKSAHEVILEFIRSRPPLNPVSARKLKPQTQPPPTLHERILEEIKAERKLRPVSPDMMRRGRLGFGKTRSMPQDLFRTGIDEFSPYMGRRTSSTLSLSNGSASPRVEPSGSQSVPQRKKLLKAPSLAELDSSDSDDELIGRTSASSSSVATSLMDDTSPESVLGKKTPPMFLPISSTPQPERRQTPQRRHSIEKETPTTVRLFQPPSKQSSKSLEEFCFPVESLSLTVEEVMHIRQVLVKAELEKFQQYKDIYNAMKKGKLCFSCRTKRFSLFTWSYTCQFCKKPVCSQCCKKMRLPSKPYSSLPIYSIGSTNTLPRDRVNAMASVGQGLSVAGGPGPSVTTGGAAASPVLKGATKAASKGAGKATGAAAAAKTDKSSGSLQRHSIQKTMSKLSKHGSLKSHEELELPPELTEDWATMEVCVDCKKFISDIIASSKHSLSLATKRARLKRKTQSFYVTSPKGAEGYRPSERTINEI

Target ORF information:

RefSeq Version	XM_017413145.2
Organism	Kryptolebias marmoratus(mangrove rivulus)
Definition	Kryptolebias marmoratus spire type actin nucleation factor 1 (spire1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017413145.2

ORF Insert Sequence:

ATGACGGAGG CTGCGGCACC GACTCTCCGG GATGAGGAGC GGCACCGCGA CGCGATGAGT 
CCGCCGAAGG AGGAGGCCGG GGCGGAGGAC CTGTCCCTGG AGGAGATCCT GAAGCTCTAC 
AGCCAGCCCA TCAACGAGGA GCAGGCATGG GCGGTGTGCT ACCAGTGCTG CCGGACGCTG 
GCGGAGGCGC ACCGGGGCAG CAGGAGCTCC GCCGCCGCCG GAGCGTCCCC GGCTCCGGCG 
GCGGCTCCGG GGAAGATATC GGGACCCGGG GATGTTAGGA TAGGGAAGGA CGGCTCTGTG 
AGGGTGGATC ACCACGGCTG TGGAGGTAAA TACAGTCCCT GCACAACAAC AGAGGTCATC 
GAGTCTCTGG GCATCATGAT CTACAAAGCT CTGGACTATG GCCTGAAGGA GAACGAGGAG 
CGGGAACTGA GTCCTCCTCT GGAGCGCCTC ATTGATCTCA TGACAAATAT GGCCGAGGCA 
GAAAAGGACG CTTGCCCCGA TGAAGGTTAT GAGGCCACAG AGGAAGAGGA GGAGGCAGAG 
GATGACCCTG ATAATATCTC CGGAGTTCAC AGCTACAGAG ATATCCTCAA GCTTTGCACT 
GTTCACCTAC CCAGTCGATC AGATGCTCCG AGTCACTACC AGGCAGTTTG TCGAGCGCTT 
TATACGGAAA CCAGAGAGCT TCGGACGTTC CTGGAGAAGA TCAAGAGTGC CAAAGAGAAC 
CTTCGTAAGA TGGAGGAGGA GTCATCCGAG GAGCCTGTCA GAGACCTGAA TGAGCTGCAG 
AATGCTGACT GGGCTCGATT CTGGGTTCAA GTGATGAGGG ACCTCCGAGA AGGAGTCAAA 
CTGAAGAAGG TCCAGGAGCG TCAGTACAAC CCTCTGCCCA TCGAGTACCA GCTGACTCCG 
TATGAGATGC TCATGGACGA CATCAGGTCC AAACGCTACA AACTGCGCAA AGTCATGGTC 
AATGGAGACA TCCCACCGAG GTTAAAGAAA AGTGCTCATG AAGTCATCCT GGAGTTCATC 
AGGTCCAGAC CGCCTCTCAA TCCTGTGTCA GCTCGTAAGC TTAAGCCTCA GACTCAGCCT 
CCTCCGACTC TGCACGAGCG GATCCTGGAG GAGATCAAGG CTGAAAGGAA ACTTCGCCCG 
GTGTCACCTG ACATGATGCG CAGGGGAAGG CTGGGTTTTG GCAAAACTCG CAGTATGCCA 
CAGGATTTGT TCCGTACAGG AATAGACGAG TTCAGTCCAT ACATGGGCCG GAGGACGTCC 
AGCACTCTGT CTCTGAGTAA CGGATCAGCC TCTCCCAGAG TAGAGCCCTC CGGTTCTCAG 
TCTGTACCTC AGAGGAAAAA ACTCCTGAAG GCTCCCAGCC TCGCCGAGCT GGACAGCTCC 
GACTCAGATG ATGAGCTGAT TGGGCGAACA TCAGCGAGCA GCTCCAGTGT GGCGACATCT 
CTGATGGATG ATACATCCCC AGAGTCTGTG CTGGGGAAGA AAACTCCACC CATGTTCCTG 
CCCATCTCCT CCACACCTCA GCCAGAGAGG CGGCAGACCC CCCAGAGGAG ACACTCCATC 
GAGAAGGAAA CACCCACCAC CGTGCGGCTG TTTCAGCCAC CTTCCAAACA AAGCTCCAAG 
TCTCTGGAGG AGTTCTGTTT CCCTGTGGAG AGCCTGTCCC TGACAGTGGA GGAGGTGATG 
CACATCAGAC AGGTGCTGGT GAAAGCAGAG CTGGAGAAGT TTCAGCAGTA CAAAGACATC 
TACAACGCCA TGAAGAAGGG AAAGCTTTGC TTCAGTTGTC GAACCAAAAG GTTTTCCCTT 
TTCACCTGGT CCTACACCTG CCAGTTCTGC AAAAAGCCTG TGTGCTCACA GTGCTGTAAA 
AAGATGCGGC TACCATCCAA ACCTTACTCC AGTCTGCCCA TTTACTCCAT TGGCTCGACC 
AACACTCTCC CCCGAGACAG AGTAAATGCC ATGGCTTCTG TAGGACAAGG ACTCTCTGTG 
GCTGGGGGTC CGGGGCCGTC AGTGACGACG GGAGGGGCTG CTGCATCCCC CGTGTTGAAA 
GGAGCTACAA AGGCAGCATC TAAAGGAGCT GGAAAAGCTA CAGGAGCCGC AGCAGCTGCC 
AAAACTGACA AATCCTCCGG GAGCCTGCAG CGTCACAGCA TTCAAAAGAC CATGTCCAAG 
TTATCAAAGC ATGGCTCATT AAAGTCTCAC GAAGAGCTGG AGCTGCCCCC GGAGCTGACG 
GAGGACTGGG CCACCATGGA GGTGTGTGTG GACTGCAAGA AGTTCATCTC CGACATAATC 
GCCTCCAGCA AGCACAGTCT GTCACTGGCC ACAAAGAGGG CTCGCTTAAA ACGCAAGACG 
CAGTCGTTTT ACGTGACGTC CCCCAAAGGA GCGGAGGGCT ACAGGCCCTC GGAGCGAACG 
ATCAACGAAA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spire1 ( XM_017413146.1 ) cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus) -> XP_017268635.1 Kryptolebias marmoratus spire type actin nucleation factor 1 (spire1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OKr09725
Clone ID Related Accession (Same CDS sequence)	XM_017413146.1 , XM_017413146.2
Accession Version	XM_017413146.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-27
Organism	Kryptolebias marmoratus(mangrove rivulus)
Product	protein spire homolog 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016094264.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Kryptolebias marmoratus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGG CTGCGGCACC GACTCTCCGG GATGAGGAGC GGCACCGCGA CGCGATGAGT 
CCGCCGAAGG AGGAGGCCGG GGCGGAGGAC CTGTCCCTGG AGGAGATCCT GAAGCTCTAC 
AGCCAGCCCA TCAACGAGGA GCAGGCATGG GCGGTGTGCT ACCAGTGCTG CCGGACGCTG 
GCGGAGGCGC ACCGGGGCAG CAGGAGCTCC GCCGCCGCCG GAGCGTCCCC GGCTCCGGCG 
GCGGCTCCGG GGAAGATATC GGGACCCGGG GATGTTAGGA TAGGGAAGGA CGGCTCTGTG 
AGGGTGGATC ACCACGGCTG TGGAGGTAAA TACAGTCCCT GCACAACAAC AGAGGTCATC 
GAGTCTCTGG GCATCATGAT CTACAAAGCT CTGGACTATG GCCTGAAGGA GAACGAGGAG 
CGGGAACTGA GTCCTCCTCT GGAGCGCCTC ATTGATCTCA TGACAAATAT GGCCGAGGCA 
GAAAAGGACG CTTGCCCCGA TGAAGGTTAT GAGGCCACAG AGGAAGAGGA GGAGGCAGAG 
GATGACCCTG ATAATATCTC CGGAGTTCAC AGCTACAGAG ATATCCTCAA GCTTTGCACT 
GTTCACCTAC CCAGTCGATC AGATGCTCCG AGTCACTACC AGGCAGTTTG TCGAGCGCTT 
TATACGGAAA CCAGAGAGCT TCGGACGTTC CTGGAGAAGA TCAAGAGTGC CAAAGAGAAC 
CTTCGTAAGA TGGAGGAGGA GTCATCCGAG GAGCCTGTCA GAGACCTGAA TGAGCTGCAG 
AATGCTGACT GGGCTCGATT CTGGGTTCAA GTGATGAGGG ACCTCCGAGA AGGAGTCAAA 
CTGAAGAAGG TCCAGGAGCG TCAGTACAAC CCTCTGCCCA TCGAGTACCA GCTGACTCCG 
TATGAGATGC TCATGGACGA CATCAGGTCC AAACGCTACA AACTGCGCAA AGTCATGGTC 
AATGGAGACA TCCCACCGAG GTTAAAGAAA AGTGCTCATG AAGTCATCCT GGAGTTCATC 
AGGTCCAGAC CGCCTCTCAA TCCTGTGTCA GCTCGTAAGC TTAAGCCTCA GACTCAGCCT 
CCTCCGACTC TGCACGAGCG GATCCTGGAG GAGATCAAGG CTGAAAGGAA ACTTCGCCCG 
GTGTCACCTG ACATGATGCG CAGGGGAAGG CTGGTAATTC GGCCACTTAG CATGTCTTTC 
AGTTTTGATG CGTCAGACGA GTTCAGTCCA TACATGGGCC GGAGGACGTC CAGCACTCTG 
TCTCTGAGTA ACGGATCAGC CTCTCCCAGA GTAGAGCCCT CCGGTTCTCA GTCTGTACCT 
CAGAGGAAAA AACTCCTGAA GGCTCCCAGC CTCGCCGAGC TGGACAGCTC CGACTCAGAT 
GATGAGCTGA TTGGGCGAAC ATCAGCGAGC AGCTCCAGTG TGGCGACATC TCTGATGGAT 
GATACATCCC CAGAGTCTGT GCTGGGGAAG AAAACTCCAC CCATGTTCCT GCCCATCTCC 
TCCACACCTC AGCCAGAGAG GCGGCAGACC CCCCAGAGGA GACACTCCAT CGAGAAGGAA 
ACACCCACCA CCGTGCGGCT GTTTCAGCCA CCTTCCAAAC AAAGCTCCAA GTCTCTGGAG 
GAGTTCTGTT TCCCTGTGGA GAGCCTGTCC CTGACAGTGG AGGAGGTGAT GCACATCAGA 
CAGGTGCTGG TGAAAGCAGA GCTGGAGAAG TTTCAGCAGT ACAAAGACAT CTACAACGCC 
ATGAAGAAGG GAAAGCTTTG CTTCAGTTGT CGAACCAAAA GGTTTTCCCT TTTCACCTGG 
TCCTACACCT GCCAGTTCTG CAAAAAGCCT GTGTGCTCAC AGTGCTGTAA AAAGATGCGG 
CTACCATCCA AACCTTACTC CAGTCTGCCC ATTTACTCCA TTGGCTCGAC CAACACTCTC 
CCCCGAGACA GAGTAAATGC CATGGCTTCT GTAGGACAAG GACTCTCTGT GGCTGGGGGT 
CCGGGGCCGT CAGTGACGAC GGGAGGGGCT GCTGCATCCC CCGTGTTGAA AGGAGCTACA 
AAGGCAGCAT CTAAAGGAGC TGGAAAAGCT ACAGGAGCCG CAGCAGCTGC CAAAACTGAC 
AAATCCTCCG GGAGCCTGCA GCGTCACAGC ATTCAAAAGA CCATGTCCAA GTTATCAAAG 
CATGGCTCAT TAAAGTCTCA CGAAGAGCTG GAGCTGCCCC CGGAGCTGAC GGAGGACTGG 
GCCACCATGG AGGTGTGTGT GGACTGCAAG AAGTTCATCT CCGACATAAT CGCCTCCAGC 
AAGCACAGTC TGTCACTGGC CACAAAGAGG GCTCGCTTAA AACGCAAGAC GCAGTCGTTT 
TACGTGACGT CCCCCAAAGG AGCGGAGGGC TACAGGCCCT CGGAGCGAAC GATCAACGAA 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017268635.1
CDS	128..2533
Translation	MTEAAAPTLRDEERHRDAMSPPKEEAGAEDLSLEEILKLYSQPINEEQAWAVCYQCCRTLAEAHRGSRSSAAAGASPAPAAAPGKISGPGDVRIGKDGSVRVDHHGCGGKYSPCTTTEVIESLGIMIYKALDYGLKENEERELSPPLERLIDLMTNMAEAEKDACPDEGYEATEEEEEAEDDPDNISGVHSYRDILKLCTVHLPSRSDAPSHYQAVCRALYTETRELRTFLEKIKSAKENLRKMEEESSEEPVRDLNELQNADWARFWVQVMRDLREGVKLKKVQERQYNPLPIEYQLTPYEMLMDDIRSKRYKLRKVMVNGDIPPRLKKSAHEVILEFIRSRPPLNPVSARKLKPQTQPPPTLHERILEEIKAERKLRPVSPDMMRRGRLVIRPLSMSFSFDASDEFSPYMGRRTSSTLSLSNGSASPRVEPSGSQSVPQRKKLLKAPSLAELDSSDSDDELIGRTSASSSSVATSLMDDTSPESVLGKKTPPMFLPISSTPQPERRQTPQRRHSIEKETPTTVRLFQPPSKQSSKSLEEFCFPVESLSLTVEEVMHIRQVLVKAELEKFQQYKDIYNAMKKGKLCFSCRTKRFSLFTWSYTCQFCKKPVCSQCCKKMRLPSKPYSSLPIYSIGSTNTLPRDRVNAMASVGQGLSVAGGPGPSVTTGGAAASPVLKGATKAASKGAGKATGAAAAAKTDKSSGSLQRHSIQKTMSKLSKHGSLKSHEELELPPELTEDWATMEVCVDCKKFISDIIASSKHSLSLATKRARLKRKTQSFYVTSPKGAEGYRPSERTINEI

Target ORF information:

RefSeq Version	XM_017413146.1
Organism	Kryptolebias marmoratus(mangrove rivulus)
Definition	Kryptolebias marmoratus spire type actin nucleation factor 1 (spire1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017413146.1

ORF Insert Sequence:

ATGACGGAGG CTGCGGCACC GACTCTCCGG GATGAGGAGC GGCACCGCGA CGCGATGAGT 
CCGCCGAAGG AGGAGGCCGG GGCGGAGGAC CTGTCCCTGG AGGAGATCCT GAAGCTCTAC 
AGCCAGCCCA TCAACGAGGA GCAGGCATGG GCGGTGTGCT ACCAGTGCTG CCGGACGCTG 
GCGGAGGCGC ACCGGGGCAG CAGGAGCTCC GCCGCCGCCG GAGCGTCCCC GGCTCCGGCG 
GCGGCTCCGG GGAAGATATC GGGACCCGGG GATGTTAGGA TAGGGAAGGA CGGCTCTGTG 
AGGGTGGATC ACCACGGCTG TGGAGGTAAA TACAGTCCCT GCACAACAAC AGAGGTCATC 
GAGTCTCTGG GCATCATGAT CTACAAAGCT CTGGACTATG GCCTGAAGGA GAACGAGGAG 
CGGGAACTGA GTCCTCCTCT GGAGCGCCTC ATTGATCTCA TGACAAATAT GGCCGAGGCA 
GAAAAGGACG CTTGCCCCGA TGAAGGTTAT GAGGCCACAG AGGAAGAGGA GGAGGCAGAG 
GATGACCCTG ATAATATCTC CGGAGTTCAC AGCTACAGAG ATATCCTCAA GCTTTGCACT 
GTTCACCTAC CCAGTCGATC AGATGCTCCG AGTCACTACC AGGCAGTTTG TCGAGCGCTT 
TATACGGAAA CCAGAGAGCT TCGGACGTTC CTGGAGAAGA TCAAGAGTGC CAAAGAGAAC 
CTTCGTAAGA TGGAGGAGGA GTCATCCGAG GAGCCTGTCA GAGACCTGAA TGAGCTGCAG 
AATGCTGACT GGGCTCGATT CTGGGTTCAA GTGATGAGGG ACCTCCGAGA AGGAGTCAAA 
CTGAAGAAGG TCCAGGAGCG TCAGTACAAC CCTCTGCCCA TCGAGTACCA GCTGACTCCG 
TATGAGATGC TCATGGACGA CATCAGGTCC AAACGCTACA AACTGCGCAA AGTCATGGTC 
AATGGAGACA TCCCACCGAG GTTAAAGAAA AGTGCTCATG AAGTCATCCT GGAGTTCATC 
AGGTCCAGAC CGCCTCTCAA TCCTGTGTCA GCTCGTAAGC TTAAGCCTCA GACTCAGCCT 
CCTCCGACTC TGCACGAGCG GATCCTGGAG GAGATCAAGG CTGAAAGGAA ACTTCGCCCG 
GTGTCACCTG ACATGATGCG CAGGGGAAGG CTGGTAATTC GGCCACTTAG CATGTCTTTC 
AGTTTTGATG CGTCAGACGA GTTCAGTCCA TACATGGGCC GGAGGACGTC CAGCACTCTG 
TCTCTGAGTA ACGGATCAGC CTCTCCCAGA GTAGAGCCCT CCGGTTCTCA GTCTGTACCT 
CAGAGGAAAA AACTCCTGAA GGCTCCCAGC CTCGCCGAGC TGGACAGCTC CGACTCAGAT 
GATGAGCTGA TTGGGCGAAC ATCAGCGAGC AGCTCCAGTG TGGCGACATC TCTGATGGAT 
GATACATCCC CAGAGTCTGT GCTGGGGAAG AAAACTCCAC CCATGTTCCT GCCCATCTCC 
TCCACACCTC AGCCAGAGAG GCGGCAGACC CCCCAGAGGA GACACTCCAT CGAGAAGGAA 
ACACCCACCA CCGTGCGGCT GTTTCAGCCA CCTTCCAAAC AAAGCTCCAA GTCTCTGGAG 
GAGTTCTGTT TCCCTGTGGA GAGCCTGTCC CTGACAGTGG AGGAGGTGAT GCACATCAGA 
CAGGTGCTGG TGAAAGCAGA GCTGGAGAAG TTTCAGCAGT ACAAAGACAT CTACAACGCC 
ATGAAGAAGG GAAAGCTTTG CTTCAGTTGT CGAACCAAAA GGTTTTCCCT TTTCACCTGG 
TCCTACACCT GCCAGTTCTG CAAAAAGCCT GTGTGCTCAC AGTGCTGTAA AAAGATGCGG 
CTACCATCCA AACCTTACTC CAGTCTGCCC ATTTACTCCA TTGGCTCGAC CAACACTCTC 
CCCCGAGACA GAGTAAATGC CATGGCTTCT GTAGGACAAG GACTCTCTGT GGCTGGGGGT 
CCGGGGCCGT CAGTGACGAC GGGAGGGGCT GCTGCATCCC CCGTGTTGAA AGGAGCTACA 
AAGGCAGCAT CTAAAGGAGC TGGAAAAGCT ACAGGAGCCG CAGCAGCTGC CAAAACTGAC 
AAATCCTCCG GGAGCCTGCA GCGTCACAGC ATTCAAAAGA CCATGTCCAA GTTATCAAAG 
CATGGCTCAT TAAAGTCTCA CGAAGAGCTG GAGCTGCCCC CGGAGCTGAC GGAGGACTGG 
GCCACCATGG AGGTGTGTGT GGACTGCAAG AAGTTCATCT CCGACATAAT CGCCTCCAGC 
AAGCACAGTC TGTCACTGGC CACAAAGAGG GCTCGCTTAA AACGCAAGAC GCAGTCGTTT 
TACGTGACGT CCCCCAAAGG AGCGGAGGGC TACAGGCCCT CGGAGCGAAC GATCAACGAA 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spire1 ( XM_017413146.2 ) cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus) -> XP_017268635.1 Kryptolebias marmoratus spire type actin nucleation factor 1 (spire1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OKr09725
Clone ID Related Accession (Same CDS sequence)	XM_017413146.1 , XM_017413146.2
Accession Version	XM_017413146.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-03
Organism	Kryptolebias marmoratus(mangrove rivulus)
Product	protein spire homolog 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016094264.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 4, 2018 this sequence version replaced XM_017413146.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Kryptolebias marmoratus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGG CTGCGGCACC GACTCTCCGG GATGAGGAGC GGCACCGCGA CGCGATGAGT 
CCGCCGAAGG AGGAGGCCGG GGCGGAGGAC CTGTCCCTGG AGGAGATCCT GAAGCTCTAC 
AGCCAGCCCA TCAACGAGGA GCAGGCATGG GCGGTGTGCT ACCAGTGCTG CCGGACGCTG 
GCGGAGGCGC ACCGGGGCAG CAGGAGCTCC GCCGCCGCCG GAGCGTCCCC GGCTCCGGCG 
GCGGCTCCGG GGAAGATATC GGGACCCGGG GATGTTAGGA TAGGGAAGGA CGGCTCTGTG 
AGGGTGGATC ACCACGGCTG TGGAGGTAAA TACAGTCCCT GCACAACAAC AGAGGTCATC 
GAGTCTCTGG GCATCATGAT CTACAAAGCT CTGGACTATG GCCTGAAGGA GAACGAGGAG 
CGGGAACTGA GTCCTCCTCT GGAGCGCCTC ATTGATCTCA TGACAAATAT GGCCGAGGCA 
GAAAAGGACG CTTGCCCCGA TGAAGGTTAT GAGGCCACAG AGGAAGAGGA GGAGGCAGAG 
GATGACCCTG ATAATATCTC CGGAGTTCAC AGCTACAGAG ATATCCTCAA GCTTTGCACT 
GTTCACCTAC CCAGTCGATC AGATGCTCCG AGTCACTACC AGGCAGTTTG TCGAGCGCTT 
TATACGGAAA CCAGAGAGCT TCGGACGTTC CTGGAGAAGA TCAAGAGTGC CAAAGAGAAC 
CTTCGTAAGA TGGAGGAGGA GTCATCCGAG GAGCCTGTCA GAGACCTGAA TGAGCTGCAG 
AATGCTGACT GGGCTCGATT CTGGGTTCAA GTGATGAGGG ACCTCCGAGA AGGAGTCAAA 
CTGAAGAAGG TCCAGGAGCG TCAGTACAAC CCTCTGCCCA TCGAGTACCA GCTGACTCCG 
TATGAGATGC TCATGGACGA CATCAGGTCC AAACGCTACA AACTGCGCAA AGTCATGGTC 
AATGGAGACA TCCCACCGAG GTTAAAGAAA AGTGCTCATG AAGTCATCCT GGAGTTCATC 
AGGTCCAGAC CGCCTCTCAA TCCTGTGTCA GCTCGTAAGC TTAAGCCTCA GACTCAGCCT 
CCTCCGACTC TGCACGAGCG GATCCTGGAG GAGATCAAGG CTGAAAGGAA ACTTCGCCCG 
GTGTCACCTG ACATGATGCG CAGGGGAAGG CTGGTAATTC GGCCACTTAG CATGTCTTTC 
AGTTTTGATG CGTCAGACGA GTTCAGTCCA TACATGGGCC GGAGGACGTC CAGCACTCTG 
TCTCTGAGTA ACGGATCAGC CTCTCCCAGA GTAGAGCCCT CCGGTTCTCA GTCTGTACCT 
CAGAGGAAAA AACTCCTGAA GGCTCCCAGC CTCGCCGAGC TGGACAGCTC CGACTCAGAT 
GATGAGCTGA TTGGGCGAAC ATCAGCGAGC AGCTCCAGTG TGGCGACATC TCTGATGGAT 
GATACATCCC CAGAGTCTGT GCTGGGGAAG AAAACTCCAC CCATGTTCCT GCCCATCTCC 
TCCACACCTC AGCCAGAGAG GCGGCAGACC CCCCAGAGGA GACACTCCAT CGAGAAGGAA 
ACACCCACCA CCGTGCGGCT GTTTCAGCCA CCTTCCAAAC AAAGCTCCAA GTCTCTGGAG 
GAGTTCTGTT TCCCTGTGGA GAGCCTGTCC CTGACAGTGG AGGAGGTGAT GCACATCAGA 
CAGGTGCTGG TGAAAGCAGA GCTGGAGAAG TTTCAGCAGT ACAAAGACAT CTACAACGCC 
ATGAAGAAGG GAAAGCTTTG CTTCAGTTGT CGAACCAAAA GGTTTTCCCT TTTCACCTGG 
TCCTACACCT GCCAGTTCTG CAAAAAGCCT GTGTGCTCAC AGTGCTGTAA AAAGATGCGG 
CTACCATCCA AACCTTACTC CAGTCTGCCC ATTTACTCCA TTGGCTCGAC CAACACTCTC 
CCCCGAGACA GAGTAAATGC CATGGCTTCT GTAGGACAAG GACTCTCTGT GGCTGGGGGT 
CCGGGGCCGT CAGTGACGAC GGGAGGGGCT GCTGCATCCC CCGTGTTGAA AGGAGCTACA 
AAGGCAGCAT CTAAAGGAGC TGGAAAAGCT ACAGGAGCCG CAGCAGCTGC CAAAACTGAC 
AAATCCTCCG GGAGCCTGCA GCGTCACAGC ATTCAAAAGA CCATGTCCAA GTTATCAAAG 
CATGGCTCAT TAAAGTCTCA CGAAGAGCTG GAGCTGCCCC CGGAGCTGAC GGAGGACTGG 
GCCACCATGG AGGTGTGTGT GGACTGCAAG AAGTTCATCT CCGACATAAT CGCCTCCAGC 
AAGCACAGTC TGTCACTGGC CACAAAGAGG GCTCGCTTAA AACGCAAGAC GCAGTCGTTT 
TACGTGACGT CCCCCAAAGG AGCGGAGGGC TACAGGCCCT CGGAGCGAAC GATCAACGAA 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017268635.1
CDS	171..2576
Translation	MTEAAAPTLRDEERHRDAMSPPKEEAGAEDLSLEEILKLYSQPINEEQAWAVCYQCCRTLAEAHRGSRSSAAAGASPAPAAAPGKISGPGDVRIGKDGSVRVDHHGCGGKYSPCTTTEVIESLGIMIYKALDYGLKENEERELSPPLERLIDLMTNMAEAEKDACPDEGYEATEEEEEAEDDPDNISGVHSYRDILKLCTVHLPSRSDAPSHYQAVCRALYTETRELRTFLEKIKSAKENLRKMEEESSEEPVRDLNELQNADWARFWVQVMRDLREGVKLKKVQERQYNPLPIEYQLTPYEMLMDDIRSKRYKLRKVMVNGDIPPRLKKSAHEVILEFIRSRPPLNPVSARKLKPQTQPPPTLHERILEEIKAERKLRPVSPDMMRRGRLVIRPLSMSFSFDASDEFSPYMGRRTSSTLSLSNGSASPRVEPSGSQSVPQRKKLLKAPSLAELDSSDSDDELIGRTSASSSSVATSLMDDTSPESVLGKKTPPMFLPISSTPQPERRQTPQRRHSIEKETPTTVRLFQPPSKQSSKSLEEFCFPVESLSLTVEEVMHIRQVLVKAELEKFQQYKDIYNAMKKGKLCFSCRTKRFSLFTWSYTCQFCKKPVCSQCCKKMRLPSKPYSSLPIYSIGSTNTLPRDRVNAMASVGQGLSVAGGPGPSVTTGGAAASPVLKGATKAASKGAGKATGAAAAAKTDKSSGSLQRHSIQKTMSKLSKHGSLKSHEELELPPELTEDWATMEVCVDCKKFISDIIASSKHSLSLATKRARLKRKTQSFYVTSPKGAEGYRPSERTINEI

Target ORF information:

RefSeq Version	XM_017413146.2
Organism	Kryptolebias marmoratus(mangrove rivulus)
Definition	Kryptolebias marmoratus spire type actin nucleation factor 1 (spire1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017413146.2

ORF Insert Sequence:

ATGACGGAGG CTGCGGCACC GACTCTCCGG GATGAGGAGC GGCACCGCGA CGCGATGAGT 
CCGCCGAAGG AGGAGGCCGG GGCGGAGGAC CTGTCCCTGG AGGAGATCCT GAAGCTCTAC 
AGCCAGCCCA TCAACGAGGA GCAGGCATGG GCGGTGTGCT ACCAGTGCTG CCGGACGCTG 
GCGGAGGCGC ACCGGGGCAG CAGGAGCTCC GCCGCCGCCG GAGCGTCCCC GGCTCCGGCG 
GCGGCTCCGG GGAAGATATC GGGACCCGGG GATGTTAGGA TAGGGAAGGA CGGCTCTGTG 
AGGGTGGATC ACCACGGCTG TGGAGGTAAA TACAGTCCCT GCACAACAAC AGAGGTCATC 
GAGTCTCTGG GCATCATGAT CTACAAAGCT CTGGACTATG GCCTGAAGGA GAACGAGGAG 
CGGGAACTGA GTCCTCCTCT GGAGCGCCTC ATTGATCTCA TGACAAATAT GGCCGAGGCA 
GAAAAGGACG CTTGCCCCGA TGAAGGTTAT GAGGCCACAG AGGAAGAGGA GGAGGCAGAG 
GATGACCCTG ATAATATCTC CGGAGTTCAC AGCTACAGAG ATATCCTCAA GCTTTGCACT 
GTTCACCTAC CCAGTCGATC AGATGCTCCG AGTCACTACC AGGCAGTTTG TCGAGCGCTT 
TATACGGAAA CCAGAGAGCT TCGGACGTTC CTGGAGAAGA TCAAGAGTGC CAAAGAGAAC 
CTTCGTAAGA TGGAGGAGGA GTCATCCGAG GAGCCTGTCA GAGACCTGAA TGAGCTGCAG 
AATGCTGACT GGGCTCGATT CTGGGTTCAA GTGATGAGGG ACCTCCGAGA AGGAGTCAAA 
CTGAAGAAGG TCCAGGAGCG TCAGTACAAC CCTCTGCCCA TCGAGTACCA GCTGACTCCG 
TATGAGATGC TCATGGACGA CATCAGGTCC AAACGCTACA AACTGCGCAA AGTCATGGTC 
AATGGAGACA TCCCACCGAG GTTAAAGAAA AGTGCTCATG AAGTCATCCT GGAGTTCATC 
AGGTCCAGAC CGCCTCTCAA TCCTGTGTCA GCTCGTAAGC TTAAGCCTCA GACTCAGCCT 
CCTCCGACTC TGCACGAGCG GATCCTGGAG GAGATCAAGG CTGAAAGGAA ACTTCGCCCG 
GTGTCACCTG ACATGATGCG CAGGGGAAGG CTGGTAATTC GGCCACTTAG CATGTCTTTC 
AGTTTTGATG CGTCAGACGA GTTCAGTCCA TACATGGGCC GGAGGACGTC CAGCACTCTG 
TCTCTGAGTA ACGGATCAGC CTCTCCCAGA GTAGAGCCCT CCGGTTCTCA GTCTGTACCT 
CAGAGGAAAA AACTCCTGAA GGCTCCCAGC CTCGCCGAGC TGGACAGCTC CGACTCAGAT 
GATGAGCTGA TTGGGCGAAC ATCAGCGAGC AGCTCCAGTG TGGCGACATC TCTGATGGAT 
GATACATCCC CAGAGTCTGT GCTGGGGAAG AAAACTCCAC CCATGTTCCT GCCCATCTCC 
TCCACACCTC AGCCAGAGAG GCGGCAGACC CCCCAGAGGA GACACTCCAT CGAGAAGGAA 
ACACCCACCA CCGTGCGGCT GTTTCAGCCA CCTTCCAAAC AAAGCTCCAA GTCTCTGGAG 
GAGTTCTGTT TCCCTGTGGA GAGCCTGTCC CTGACAGTGG AGGAGGTGAT GCACATCAGA 
CAGGTGCTGG TGAAAGCAGA GCTGGAGAAG TTTCAGCAGT ACAAAGACAT CTACAACGCC 
ATGAAGAAGG GAAAGCTTTG CTTCAGTTGT CGAACCAAAA GGTTTTCCCT TTTCACCTGG 
TCCTACACCT GCCAGTTCTG CAAAAAGCCT GTGTGCTCAC AGTGCTGTAA AAAGATGCGG 
CTACCATCCA AACCTTACTC CAGTCTGCCC ATTTACTCCA TTGGCTCGAC CAACACTCTC 
CCCCGAGACA GAGTAAATGC CATGGCTTCT GTAGGACAAG GACTCTCTGT GGCTGGGGGT 
CCGGGGCCGT CAGTGACGAC GGGAGGGGCT GCTGCATCCC CCGTGTTGAA AGGAGCTACA 
AAGGCAGCAT CTAAAGGAGC TGGAAAAGCT ACAGGAGCCG CAGCAGCTGC CAAAACTGAC 
AAATCCTCCG GGAGCCTGCA GCGTCACAGC ATTCAAAAGA CCATGTCCAA GTTATCAAAG 
CATGGCTCAT TAAAGTCTCA CGAAGAGCTG GAGCTGCCCC CGGAGCTGAC GGAGGACTGG 
GCCACCATGG AGGTGTGTGT GGACTGCAAG AAGTTCATCT CCGACATAAT CGCCTCCAGC 
AAGCACAGTC TGTCACTGGC CACAAAGAGG GCTCGCTTAA AACGCAAGAC GCAGTCGTTT 
TACGTGACGT CCCCCAAAGG AGCGGAGGGC TACAGGCCCT CGGAGCGAAC GATCAACGAA 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spire1 ( XM_017413147.2 ) cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus) -> XP_017268636.1 Kryptolebias marmoratus spire type actin nucleation factor 1 (spire1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OKr09726
Clone ID Related Accession (Same CDS sequence)	XM_017413147.2 , XM_017413147.1
Accession Version	XM_017413147.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-03
Organism	Kryptolebias marmoratus(mangrove rivulus)
Product	protein spire homolog 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016094264.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 4, 2018 this sequence version replaced XM_017413147.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Kryptolebias marmoratus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGG CTGCGGCACC GACTCTCCGG GATGAGGAGC GGCACCGCGA CGCGATGAGT 
CCGCCGAAGG AGGAGGCCGG GGCGGAGGAC CTGTCCCTGG AGGAGATCCT GAAGCTCTAC 
AGCCAGCCCA TCAACGAGGA GCAGGCATGG GCGGTGTGCT ACCAGTGCTG CCGGACGCTG 
GCGGAGGCGC ACCGGGGCAG CAGGAGCTCC GCCGCCGCCG GAGCGTCCCC GGCTCCGGCG 
GCGGCTCCGG GGAAGATATC GGGACCCGGG GATGTTAGGA TAGGGAAGGA CGGCTCTGTG 
AGGGTGGATC ACCACGGCTG TGGAGGTAAA TACAGTCCCT GCACAACAAC AGAGGTCATC 
GAGTCTCTGG GCATCATGAT CTACAAAGCT CTGGACTATG GCCTGAAGGA GAACGAGGAG 
CGGGAACTGA GTCCTCCTCT GGAGCGCCTC ATTGATCTCA TGACAAATAT GGCCGAGGCA 
GAAAAGGACG CTTGCCCCGA TGAAGGTTAT GAGGCCACAG AGGAAGAGGA GGAGGCAGAG 
GATGACCCTG ATAATATCTC CGGAGTTCAC AGCTACAGAG ATATCCTCAA GCTTTGCACT 
GTTCACCTAC CCAGTCGATC AGATGCTCCG AGTCACTACC AGGCAGTTTG TCGAGCGCTT 
TATACGGAAA CCAGAGAGCT TCGGACGTTC CTGGAGAAGA TCAAGAGTGC CAAAGAGAAC 
CTTCGTAAGA TGGAGGAGGA GTCATCCGAG GAGCCTGTCA GAGACCTGAA TGAGCTGCAG 
AATGCTGACT GGGCTCGATT CTGGGTTCAA GTGATGAGGG ACCTCCGAGA AGGAGTCAAA 
CTGAAGAAGG TCCAGGAGCG TCAGTACAAC CCTCTGCCCA TCGAGTACCA GCTGACTCCG 
TATGAGATGC TCATGGACGA CATCAGGTCC AAACGCTACA AACTGCGCAA AGTCATGGTC 
AATGGAGACA TCCCACCGAG GTTAAAGAAA AGTGCTCATG AAGTCATCCT GGAGTTCATC 
AGGTCCAGAC CGCCTCTCAA TCCTGTGTCA GCTCGTAAGC TTAAGCCTCA GACTCAGCCT 
CCTCCGACTC TGCACGAGCG GATCCTGGAG GAGATCAAGG CTGAAAGGAA ACTTCGCCCG 
GTGTCACCTG ACATGATGCG CAGGGGAAGG CTGGACGAGT TCAGTCCATA CATGGGCCGG 
AGGACGTCCA GCACTCTGTC TCTGAGTAAC GGATCAGCCT CTCCCAGAGT AGAGCCCTCC 
GGTTCTCAGT CTGTACCTCA GAGGAAAAAA CTCCTGAAGG CTCCCAGCCT CGCCGAGCTG 
GACAGCTCCG ACTCAGATGA TGAGCTGATT GGGCGAACAT CAGCGAGCAG CTCCAGTGTG 
GCGACATCTC TGATGGATGA TACATCCCCA GAGTCTGTGC TGGGGAAGAA AACTCCACCC 
ATGTTCCTGC CCATCTCCTC CACACCTCAG CCAGAGAGGC GGCAGACCCC CCAGAGGAGA 
CACTCCATCG AGAAGGAAAC ACCCACCACC GTGCGGCTGT TTCAGCCACC TTCCAAACAA 
AGCTCCAAGT CTCTGGAGGA GTTCTGTTTC CCTGTGGAGA GCCTGTCCCT GACAGTGGAG 
GAGGTGATGC ACATCAGACA GGTGCTGGTG AAAGCAGAGC TGGAGAAGTT TCAGCAGTAC 
AAAGACATCT ACAACGCCAT GAAGAAGGGA AAGCTTTGCT TCAGTTGTCG AACCAAAAGG 
TTTTCCCTTT TCACCTGGTC CTACACCTGC CAGTTCTGCA AAAAGCCTGT GTGCTCACAG 
TGCTGTAAAA AGATGCGGCT ACCATCCAAA CCTTACTCCA GTCTGCCCAT TTACTCCATT 
GGCTCGACCA ACACTCTCCC CCGAGACAGA GTAAATGCCA TGGCTTCTGT AGGACAAGGA 
CTCTCTGTGG CTGGGGGTCC GGGGCCGTCA GTGACGACGG GAGGGGCTGC TGCATCCCCC 
GTGTTGAAAG GAGCTACAAA GGCAGCATCT AAAGGAGCTG GAAAAGCTAC AGGAGCCGCA 
GCAGCTGCCA AAACTGACAA ATCCTCCGGG AGCCTGCAGC GTCACAGCAT TCAAAAGACC 
ATGTCCAAGT TATCAAAGCA TGGCTCATTA AAGTCTCACG AAGAGCTGGA GCTGCCCCCG 
GAGCTGACGG AGGACTGGGC CACCATGGAG GTGTGTGTGG ACTGCAAGAA GTTCATCTCC 
GACATAATCG CCTCCAGCAA GCACAGTCTG TCACTGGCCA CAAAGAGGGC TCGCTTAAAA 
CGCAAGACGC AGTCGTTTTA CGTGACGTCC CCCAAAGGAG CGGAGGGCTA CAGGCCCTCG 
GAGCGAACGA TCAACGAAAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017268636.1
CDS	170..2533
Translation	MTEAAAPTLRDEERHRDAMSPPKEEAGAEDLSLEEILKLYSQPINEEQAWAVCYQCCRTLAEAHRGSRSSAAAGASPAPAAAPGKISGPGDVRIGKDGSVRVDHHGCGGKYSPCTTTEVIESLGIMIYKALDYGLKENEERELSPPLERLIDLMTNMAEAEKDACPDEGYEATEEEEEAEDDPDNISGVHSYRDILKLCTVHLPSRSDAPSHYQAVCRALYTETRELRTFLEKIKSAKENLRKMEEESSEEPVRDLNELQNADWARFWVQVMRDLREGVKLKKVQERQYNPLPIEYQLTPYEMLMDDIRSKRYKLRKVMVNGDIPPRLKKSAHEVILEFIRSRPPLNPVSARKLKPQTQPPPTLHERILEEIKAERKLRPVSPDMMRRGRLDEFSPYMGRRTSSTLSLSNGSASPRVEPSGSQSVPQRKKLLKAPSLAELDSSDSDDELIGRTSASSSSVATSLMDDTSPESVLGKKTPPMFLPISSTPQPERRQTPQRRHSIEKETPTTVRLFQPPSKQSSKSLEEFCFPVESLSLTVEEVMHIRQVLVKAELEKFQQYKDIYNAMKKGKLCFSCRTKRFSLFTWSYTCQFCKKPVCSQCCKKMRLPSKPYSSLPIYSIGSTNTLPRDRVNAMASVGQGLSVAGGPGPSVTTGGAAASPVLKGATKAASKGAGKATGAAAAAKTDKSSGSLQRHSIQKTMSKLSKHGSLKSHEELELPPELTEDWATMEVCVDCKKFISDIIASSKHSLSLATKRARLKRKTQSFYVTSPKGAEGYRPSERTINEI

Target ORF information:

RefSeq Version	XM_017413147.2
Organism	Kryptolebias marmoratus(mangrove rivulus)
Definition	Kryptolebias marmoratus spire type actin nucleation factor 1 (spire1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017413147.2

ORF Insert Sequence:

ATGACGGAGG CTGCGGCACC GACTCTCCGG GATGAGGAGC GGCACCGCGA CGCGATGAGT 
CCGCCGAAGG AGGAGGCCGG GGCGGAGGAC CTGTCCCTGG AGGAGATCCT GAAGCTCTAC 
AGCCAGCCCA TCAACGAGGA GCAGGCATGG GCGGTGTGCT ACCAGTGCTG CCGGACGCTG 
GCGGAGGCGC ACCGGGGCAG CAGGAGCTCC GCCGCCGCCG GAGCGTCCCC GGCTCCGGCG 
GCGGCTCCGG GGAAGATATC GGGACCCGGG GATGTTAGGA TAGGGAAGGA CGGCTCTGTG 
AGGGTGGATC ACCACGGCTG TGGAGGTAAA TACAGTCCCT GCACAACAAC AGAGGTCATC 
GAGTCTCTGG GCATCATGAT CTACAAAGCT CTGGACTATG GCCTGAAGGA GAACGAGGAG 
CGGGAACTGA GTCCTCCTCT GGAGCGCCTC ATTGATCTCA TGACAAATAT GGCCGAGGCA 
GAAAAGGACG CTTGCCCCGA TGAAGGTTAT GAGGCCACAG AGGAAGAGGA GGAGGCAGAG 
GATGACCCTG ATAATATCTC CGGAGTTCAC AGCTACAGAG ATATCCTCAA GCTTTGCACT 
GTTCACCTAC CCAGTCGATC AGATGCTCCG AGTCACTACC AGGCAGTTTG TCGAGCGCTT 
TATACGGAAA CCAGAGAGCT TCGGACGTTC CTGGAGAAGA TCAAGAGTGC CAAAGAGAAC 
CTTCGTAAGA TGGAGGAGGA GTCATCCGAG GAGCCTGTCA GAGACCTGAA TGAGCTGCAG 
AATGCTGACT GGGCTCGATT CTGGGTTCAA GTGATGAGGG ACCTCCGAGA AGGAGTCAAA 
CTGAAGAAGG TCCAGGAGCG TCAGTACAAC CCTCTGCCCA TCGAGTACCA GCTGACTCCG 
TATGAGATGC TCATGGACGA CATCAGGTCC AAACGCTACA AACTGCGCAA AGTCATGGTC 
AATGGAGACA TCCCACCGAG GTTAAAGAAA AGTGCTCATG AAGTCATCCT GGAGTTCATC 
AGGTCCAGAC CGCCTCTCAA TCCTGTGTCA GCTCGTAAGC TTAAGCCTCA GACTCAGCCT 
CCTCCGACTC TGCACGAGCG GATCCTGGAG GAGATCAAGG CTGAAAGGAA ACTTCGCCCG 
GTGTCACCTG ACATGATGCG CAGGGGAAGG CTGGACGAGT TCAGTCCATA CATGGGCCGG 
AGGACGTCCA GCACTCTGTC TCTGAGTAAC GGATCAGCCT CTCCCAGAGT AGAGCCCTCC 
GGTTCTCAGT CTGTACCTCA GAGGAAAAAA CTCCTGAAGG CTCCCAGCCT CGCCGAGCTG 
GACAGCTCCG ACTCAGATGA TGAGCTGATT GGGCGAACAT CAGCGAGCAG CTCCAGTGTG 
GCGACATCTC TGATGGATGA TACATCCCCA GAGTCTGTGC TGGGGAAGAA AACTCCACCC 
ATGTTCCTGC CCATCTCCTC CACACCTCAG CCAGAGAGGC GGCAGACCCC CCAGAGGAGA 
CACTCCATCG AGAAGGAAAC ACCCACCACC GTGCGGCTGT TTCAGCCACC TTCCAAACAA 
AGCTCCAAGT CTCTGGAGGA GTTCTGTTTC CCTGTGGAGA GCCTGTCCCT GACAGTGGAG 
GAGGTGATGC ACATCAGACA GGTGCTGGTG AAAGCAGAGC TGGAGAAGTT TCAGCAGTAC 
AAAGACATCT ACAACGCCAT GAAGAAGGGA AAGCTTTGCT TCAGTTGTCG AACCAAAAGG 
TTTTCCCTTT TCACCTGGTC CTACACCTGC CAGTTCTGCA AAAAGCCTGT GTGCTCACAG 
TGCTGTAAAA AGATGCGGCT ACCATCCAAA CCTTACTCCA GTCTGCCCAT TTACTCCATT 
GGCTCGACCA ACACTCTCCC CCGAGACAGA GTAAATGCCA TGGCTTCTGT AGGACAAGGA 
CTCTCTGTGG CTGGGGGTCC GGGGCCGTCA GTGACGACGG GAGGGGCTGC TGCATCCCCC 
GTGTTGAAAG GAGCTACAAA GGCAGCATCT AAAGGAGCTG GAAAAGCTAC AGGAGCCGCA 
GCAGCTGCCA AAACTGACAA ATCCTCCGGG AGCCTGCAGC GTCACAGCAT TCAAAAGACC 
ATGTCCAAGT TATCAAAGCA TGGCTCATTA AAGTCTCACG AAGAGCTGGA GCTGCCCCCG 
GAGCTGACGG AGGACTGGGC CACCATGGAG GTGTGTGTGG ACTGCAAGAA GTTCATCTCC 
GACATAATCG CCTCCAGCAA GCACAGTCTG TCACTGGCCA CAAAGAGGGC TCGCTTAAAA 
CGCAAGACGC AGTCGTTTTA CGTGACGTCC CCCAAAGGAG CGGAGGGCTA CAGGCCCTCG 
GAGCGAACGA TCAACGAAAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spire1 ( XM_017413147.1 ) cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus) -> XP_017268636.1 Kryptolebias marmoratus spire type actin nucleation factor 1 (spire1), transcript variant X5, mRNA.

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CloneID	OKr09726
Clone ID Related Accession (Same CDS sequence)	XM_017413147.2 , XM_017413147.1
Accession Version	XM_017413147.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-27
Organism	Kryptolebias marmoratus(mangrove rivulus)
Product	protein spire homolog 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016094264.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Kryptolebias marmoratus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGG CTGCGGCACC GACTCTCCGG GATGAGGAGC GGCACCGCGA CGCGATGAGT 
CCGCCGAAGG AGGAGGCCGG GGCGGAGGAC CTGTCCCTGG AGGAGATCCT GAAGCTCTAC 
AGCCAGCCCA TCAACGAGGA GCAGGCATGG GCGGTGTGCT ACCAGTGCTG CCGGACGCTG 
GCGGAGGCGC ACCGGGGCAG CAGGAGCTCC GCCGCCGCCG GAGCGTCCCC GGCTCCGGCG 
GCGGCTCCGG GGAAGATATC GGGACCCGGG GATGTTAGGA TAGGGAAGGA CGGCTCTGTG 
AGGGTGGATC ACCACGGCTG TGGAGGTAAA TACAGTCCCT GCACAACAAC AGAGGTCATC 
GAGTCTCTGG GCATCATGAT CTACAAAGCT CTGGACTATG GCCTGAAGGA GAACGAGGAG 
CGGGAACTGA GTCCTCCTCT GGAGCGCCTC ATTGATCTCA TGACAAATAT GGCCGAGGCA 
GAAAAGGACG CTTGCCCCGA TGAAGGTTAT GAGGCCACAG AGGAAGAGGA GGAGGCAGAG 
GATGACCCTG ATAATATCTC CGGAGTTCAC AGCTACAGAG ATATCCTCAA GCTTTGCACT 
GTTCACCTAC CCAGTCGATC AGATGCTCCG AGTCACTACC AGGCAGTTTG TCGAGCGCTT 
TATACGGAAA CCAGAGAGCT TCGGACGTTC CTGGAGAAGA TCAAGAGTGC CAAAGAGAAC 
CTTCGTAAGA TGGAGGAGGA GTCATCCGAG GAGCCTGTCA GAGACCTGAA TGAGCTGCAG 
AATGCTGACT GGGCTCGATT CTGGGTTCAA GTGATGAGGG ACCTCCGAGA AGGAGTCAAA 
CTGAAGAAGG TCCAGGAGCG TCAGTACAAC CCTCTGCCCA TCGAGTACCA GCTGACTCCG 
TATGAGATGC TCATGGACGA CATCAGGTCC AAACGCTACA AACTGCGCAA AGTCATGGTC 
AATGGAGACA TCCCACCGAG GTTAAAGAAA AGTGCTCATG AAGTCATCCT GGAGTTCATC 
AGGTCCAGAC CGCCTCTCAA TCCTGTGTCA GCTCGTAAGC TTAAGCCTCA GACTCAGCCT 
CCTCCGACTC TGCACGAGCG GATCCTGGAG GAGATCAAGG CTGAAAGGAA ACTTCGCCCG 
GTGTCACCTG ACATGATGCG CAGGGGAAGG CTGGACGAGT TCAGTCCATA CATGGGCCGG 
AGGACGTCCA GCACTCTGTC TCTGAGTAAC GGATCAGCCT CTCCCAGAGT AGAGCCCTCC 
GGTTCTCAGT CTGTACCTCA GAGGAAAAAA CTCCTGAAGG CTCCCAGCCT CGCCGAGCTG 
GACAGCTCCG ACTCAGATGA TGAGCTGATT GGGCGAACAT CAGCGAGCAG CTCCAGTGTG 
GCGACATCTC TGATGGATGA TACATCCCCA GAGTCTGTGC TGGGGAAGAA AACTCCACCC 
ATGTTCCTGC CCATCTCCTC CACACCTCAG CCAGAGAGGC GGCAGACCCC CCAGAGGAGA 
CACTCCATCG AGAAGGAAAC ACCCACCACC GTGCGGCTGT TTCAGCCACC TTCCAAACAA 
AGCTCCAAGT CTCTGGAGGA GTTCTGTTTC CCTGTGGAGA GCCTGTCCCT GACAGTGGAG 
GAGGTGATGC ACATCAGACA GGTGCTGGTG AAAGCAGAGC TGGAGAAGTT TCAGCAGTAC 
AAAGACATCT ACAACGCCAT GAAGAAGGGA AAGCTTTGCT TCAGTTGTCG AACCAAAAGG 
TTTTCCCTTT TCACCTGGTC CTACACCTGC CAGTTCTGCA AAAAGCCTGT GTGCTCACAG 
TGCTGTAAAA AGATGCGGCT ACCATCCAAA CCTTACTCCA GTCTGCCCAT TTACTCCATT 
GGCTCGACCA ACACTCTCCC CCGAGACAGA GTAAATGCCA TGGCTTCTGT AGGACAAGGA 
CTCTCTGTGG CTGGGGGTCC GGGGCCGTCA GTGACGACGG GAGGGGCTGC TGCATCCCCC 
GTGTTGAAAG GAGCTACAAA GGCAGCATCT AAAGGAGCTG GAAAAGCTAC AGGAGCCGCA 
GCAGCTGCCA AAACTGACAA ATCCTCCGGG AGCCTGCAGC GTCACAGCAT TCAAAAGACC 
ATGTCCAAGT TATCAAAGCA TGGCTCATTA AAGTCTCACG AAGAGCTGGA GCTGCCCCCG 
GAGCTGACGG AGGACTGGGC CACCATGGAG GTGTGTGTGG ACTGCAAGAA GTTCATCTCC 
GACATAATCG CCTCCAGCAA GCACAGTCTG TCACTGGCCA CAAAGAGGGC TCGCTTAAAA 
CGCAAGACGC AGTCGTTTTA CGTGACGTCC CCCAAAGGAG CGGAGGGCTA CAGGCCCTCG 
GAGCGAACGA TCAACGAAAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017268636.1
CDS	128..2491
Translation	MTEAAAPTLRDEERHRDAMSPPKEEAGAEDLSLEEILKLYSQPINEEQAWAVCYQCCRTLAEAHRGSRSSAAAGASPAPAAAPGKISGPGDVRIGKDGSVRVDHHGCGGKYSPCTTTEVIESLGIMIYKALDYGLKENEERELSPPLERLIDLMTNMAEAEKDACPDEGYEATEEEEEAEDDPDNISGVHSYRDILKLCTVHLPSRSDAPSHYQAVCRALYTETRELRTFLEKIKSAKENLRKMEEESSEEPVRDLNELQNADWARFWVQVMRDLREGVKLKKVQERQYNPLPIEYQLTPYEMLMDDIRSKRYKLRKVMVNGDIPPRLKKSAHEVILEFIRSRPPLNPVSARKLKPQTQPPPTLHERILEEIKAERKLRPVSPDMMRRGRLDEFSPYMGRRTSSTLSLSNGSASPRVEPSGSQSVPQRKKLLKAPSLAELDSSDSDDELIGRTSASSSSVATSLMDDTSPESVLGKKTPPMFLPISSTPQPERRQTPQRRHSIEKETPTTVRLFQPPSKQSSKSLEEFCFPVESLSLTVEEVMHIRQVLVKAELEKFQQYKDIYNAMKKGKLCFSCRTKRFSLFTWSYTCQFCKKPVCSQCCKKMRLPSKPYSSLPIYSIGSTNTLPRDRVNAMASVGQGLSVAGGPGPSVTTGGAAASPVLKGATKAASKGAGKATGAAAAAKTDKSSGSLQRHSIQKTMSKLSKHGSLKSHEELELPPELTEDWATMEVCVDCKKFISDIIASSKHSLSLATKRARLKRKTQSFYVTSPKGAEGYRPSERTINEI

Target ORF information:

RefSeq Version	XM_017413147.1
Organism	Kryptolebias marmoratus(mangrove rivulus)
Definition	Kryptolebias marmoratus spire type actin nucleation factor 1 (spire1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017413147.1

ORF Insert Sequence:

ATGACGGAGG CTGCGGCACC GACTCTCCGG GATGAGGAGC GGCACCGCGA CGCGATGAGT 
CCGCCGAAGG AGGAGGCCGG GGCGGAGGAC CTGTCCCTGG AGGAGATCCT GAAGCTCTAC 
AGCCAGCCCA TCAACGAGGA GCAGGCATGG GCGGTGTGCT ACCAGTGCTG CCGGACGCTG 
GCGGAGGCGC ACCGGGGCAG CAGGAGCTCC GCCGCCGCCG GAGCGTCCCC GGCTCCGGCG 
GCGGCTCCGG GGAAGATATC GGGACCCGGG GATGTTAGGA TAGGGAAGGA CGGCTCTGTG 
AGGGTGGATC ACCACGGCTG TGGAGGTAAA TACAGTCCCT GCACAACAAC AGAGGTCATC 
GAGTCTCTGG GCATCATGAT CTACAAAGCT CTGGACTATG GCCTGAAGGA GAACGAGGAG 
CGGGAACTGA GTCCTCCTCT GGAGCGCCTC ATTGATCTCA TGACAAATAT GGCCGAGGCA 
GAAAAGGACG CTTGCCCCGA TGAAGGTTAT GAGGCCACAG AGGAAGAGGA GGAGGCAGAG 
GATGACCCTG ATAATATCTC CGGAGTTCAC AGCTACAGAG ATATCCTCAA GCTTTGCACT 
GTTCACCTAC CCAGTCGATC AGATGCTCCG AGTCACTACC AGGCAGTTTG TCGAGCGCTT 
TATACGGAAA CCAGAGAGCT TCGGACGTTC CTGGAGAAGA TCAAGAGTGC CAAAGAGAAC 
CTTCGTAAGA TGGAGGAGGA GTCATCCGAG GAGCCTGTCA GAGACCTGAA TGAGCTGCAG 
AATGCTGACT GGGCTCGATT CTGGGTTCAA GTGATGAGGG ACCTCCGAGA AGGAGTCAAA 
CTGAAGAAGG TCCAGGAGCG TCAGTACAAC CCTCTGCCCA TCGAGTACCA GCTGACTCCG 
TATGAGATGC TCATGGACGA CATCAGGTCC AAACGCTACA AACTGCGCAA AGTCATGGTC 
AATGGAGACA TCCCACCGAG GTTAAAGAAA AGTGCTCATG AAGTCATCCT GGAGTTCATC 
AGGTCCAGAC CGCCTCTCAA TCCTGTGTCA GCTCGTAAGC TTAAGCCTCA GACTCAGCCT 
CCTCCGACTC TGCACGAGCG GATCCTGGAG GAGATCAAGG CTGAAAGGAA ACTTCGCCCG 
GTGTCACCTG ACATGATGCG CAGGGGAAGG CTGGACGAGT TCAGTCCATA CATGGGCCGG 
AGGACGTCCA GCACTCTGTC TCTGAGTAAC GGATCAGCCT CTCCCAGAGT AGAGCCCTCC 
GGTTCTCAGT CTGTACCTCA GAGGAAAAAA CTCCTGAAGG CTCCCAGCCT CGCCGAGCTG 
GACAGCTCCG ACTCAGATGA TGAGCTGATT GGGCGAACAT CAGCGAGCAG CTCCAGTGTG 
GCGACATCTC TGATGGATGA TACATCCCCA GAGTCTGTGC TGGGGAAGAA AACTCCACCC 
ATGTTCCTGC CCATCTCCTC CACACCTCAG CCAGAGAGGC GGCAGACCCC CCAGAGGAGA 
CACTCCATCG AGAAGGAAAC ACCCACCACC GTGCGGCTGT TTCAGCCACC TTCCAAACAA 
AGCTCCAAGT CTCTGGAGGA GTTCTGTTTC CCTGTGGAGA GCCTGTCCCT GACAGTGGAG 
GAGGTGATGC ACATCAGACA GGTGCTGGTG AAAGCAGAGC TGGAGAAGTT TCAGCAGTAC 
AAAGACATCT ACAACGCCAT GAAGAAGGGA AAGCTTTGCT TCAGTTGTCG AACCAAAAGG 
TTTTCCCTTT TCACCTGGTC CTACACCTGC CAGTTCTGCA AAAAGCCTGT GTGCTCACAG 
TGCTGTAAAA AGATGCGGCT ACCATCCAAA CCTTACTCCA GTCTGCCCAT TTACTCCATT 
GGCTCGACCA ACACTCTCCC CCGAGACAGA GTAAATGCCA TGGCTTCTGT AGGACAAGGA 
CTCTCTGTGG CTGGGGGTCC GGGGCCGTCA GTGACGACGG GAGGGGCTGC TGCATCCCCC 
GTGTTGAAAG GAGCTACAAA GGCAGCATCT AAAGGAGCTG GAAAAGCTAC AGGAGCCGCA 
GCAGCTGCCA AAACTGACAA ATCCTCCGGG AGCCTGCAGC GTCACAGCAT TCAAAAGACC 
ATGTCCAAGT TATCAAAGCA TGGCTCATTA AAGTCTCACG AAGAGCTGGA GCTGCCCCCG 
GAGCTGACGG AGGACTGGGC CACCATGGAG GTGTGTGTGG ACTGCAAGAA GTTCATCTCC 
GACATAATCG CCTCCAGCAA GCACAGTCTG TCACTGGCCA CAAAGAGGGC TCGCTTAAAA 
CGCAAGACGC AGTCGTTTTA CGTGACGTCC CCCAAAGGAG CGGAGGGCTA CAGGCCCTCG 
GAGCGAACGA TCAACGAAAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	spire1
Entrez Gene ID	108234180
Full Name	spire type actin nucleation factor 1
Gene Type	protein-coding
Organism	Kryptolebias marmoratus(mangrove rivulus)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

spire1 cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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