LOC108232031 gene cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus)

mRNA	Protein	Name
XM_017409522.1	XP_017265011.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X5
XM_017409525.1	XP_017265014.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X8
XM_017409526.1	XP_017265015.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X9
XM_017409522.2	XP_017265011.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X6
XM_017409524.2	XP_017265013.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X8
XM_025004509.1	XP_024860277.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X10
XM_017409520.1	XP_017265009.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X4
XM_017409519.1	XP_017265008.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X3
XM_017409525.2	XP_017265014.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X9
XM_017409518.1	XP_017265007.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X2
XM_017409523.1	XP_017265012.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X6
XM_017409517.2	XP_017265006.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X1
XM_017409518.2	XP_017265007.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X2
XM_017409520.2	XP_017265009.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X5
XM_025004508.1	XP_024860276.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X7
XM_017409517.1	XP_017265006.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X1
XM_017409524.1	XP_017265013.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X7
XM_017409519.2	XP_017265008.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X3
XM_025004507.1	XP_024860275.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OKr06775	XM_017409525.2 Latest version!	Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OKr06776	XM_017409526.1 Latest version!	Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OKr34862	XM_025004509.1 Latest version!	Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OKr06768	XM_017409517.2 Latest version!	Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OKr06769	XM_017409518.2 Latest version!	Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OKr06770	XM_017409519.2 Latest version!	Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OKr06771	XM_017409520.2 Latest version!	Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OKr06772	XM_017409522.2 Latest version!	Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OKr06773	XM_017409523.1 Latest version!	Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OKr06774	XM_017409524.2 Latest version!	Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OKr34860	XM_025004507.1 Latest version!	Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OKr34861	XM_025004508.1 Latest version!	Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OKr06772	XM_017409522.1	Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OKr06775	XM_017409525.1	Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OKr06769	XM_017409518.1	Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OKr06771	XM_017409520.1	Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OKr06768	XM_017409517.1	Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OKr06774	XM_017409524.1	Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OKr06770	XM_017409519.1	Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
Hide Old Accession Versions >>

LOC108232031 ( XM_017409525.1 ) cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus) -> XP_017265014.1 Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OKr06775
Clone ID Related Accession (Same CDS sequence)	XM_017409525.1 , XM_017409525.2
Accession Version	XM_017409525.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-27
Organism	Kryptolebias marmoratus(mangrove rivulus)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016094253.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Kryptolebias marmoratus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACGA AAGTGAAGGA GTGCGCCGTG 
GTTCGGGACC CCAGCGGGGA GCTGAACTTG GAGCTGCGCG GCGGGGCGCA GGACGGAGAG 
TTCGCCTACA TCGGACACAT CAGGGAGGAT GCTGTGCTCC ACCAGGACGG GAAACTGTCT 
GAGGGAGAGC TGCTGCTGGA GGTGGAGGGG CTGTCCGTGT CTGGATTACC TCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAAGGT CCCGTCAGGT TCAAAACTGT CCGCCAAGGT 
CACAAGCTCA ACAAGGACCT AAAGCACTTC CTGAGCTTGA GGTTTCAGAA GTCATCACCT 
GACCACGAGT TGCAGAAGAC TATCCGAGCC AATCTATATC GTCATGCCGT GCCTTGTACA 
ACCCGCGCCC CTCGAGACGG AGAGGTGCCA GGGGTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTTGAGGA GAGTGGCACA CTACTGGAAA TAGGAACGTA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC GCCGAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
ACGGGCTCCC ATCAGTCCAC CCCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAACGTC 
CGCGTAGTGC CTGCTGACGA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGAACCT TAGTGCGTAG CCTTTTCCCC TCTCCTTTGT TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGTAC CGGCATCCTT CGGCCATATC CTGTGCGTGA TAATGCTCTG 
GCCTGTGGTC TGAACAATGC CACCACCTCC ACTGTTGAAA GTGGGCAGCA GACGCTCGCA 
GAACCACAGG TCTCTCCAGA TGACCCTCTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACTGAGA ACGGGGAGCT GTACTTCATA GACACTGCAA AGCCTCACCA CCAGGACATG 
GCTGTGCATT TTCAGAGTGT CCACAACACA AAGACCACAT CATGGCTGGA TCCGCGGTGC 
CGAGACAAAG CCTCCAGGCC TTTGGAAGAG TGTGATGATG ATGAAGAAGG GATACATACG 
GAAGACCTGG AAAGTGATCT AGAGCTGCCC CCAGGATGGG AAAGAATAGA TGACCCCGTG 
TACGGAGTGT ACTATGTAGA TCATATAAAC AGGAAGACCC AATATGAGAA CCCAGTGGTG 
GAGGCGCGGC GCCAAAAACT CTTGGAGCAG CAGCAACAGC CGCAGCCTCC AGAAGGTGAG 
CGGTATATTC GAGAGTGGAT AGAGGAGCAC TCAGCTGCAG CAGCTCCTTT GGCAAACTAT 
GCTCCAAACC ACCTGGAGAC CTACAGGGAC CCACAAGTCC CTCCAGCTCT ACCTCCAGGC 
CCCGCTGGCA TCAAAAGAGG GAAACCTTTC TTTACGAGAA ACCCAGCAGA GCTGAAGGGA 
ACCTTCATCA ACACCAAGCT GAAGAAAAGT CACCGTGGAT TCGGGTTCAC CGTGGTGGGA 
GGTGACGAGC CCGACGAGTT CCTGCAGATC AAGAGTCTGG TGCTGGATGG GCCTGCGGCT 
GTTGATGGCA AGATGGAGAC AGGTGATGTG ATTGTCAGCG TGAATGACAC TATTGTGCTG 
GGCTACACTC ATGCTCAGGT GGTGAAGATC TTCCAGTCCA TCCCTATCGG CTCCATGGTG 
GACCTGGCCT TATGCCGTGG CTACCCACTG CCCTTTGACC CAGACGACCC AAACACCAGC 
CTGGTGACCT CTGTGGCCAT CTTGGACAAG GAACCCATTA TTGTCAATGG GCAAGAGACG 
TTTGACACGC CGCCTAACCA GGCTGGACCC CTTAACAGCA TGCGTGAGCC ACGGCCTCTC 
AGCCCCTCAG CTGAGGTGGC GTCCAACGGC TCCCATGGTT ACTCCAGCGA CGTGGTCACG 
TTGGCCTCGT CCATAGCCAC TCAGCCCGAG CTCATCACCA TCCACATGGA GAAGGGGGAC 
AAAGGTTTTG GCTTCACCAT CGCTGACAGC CTAACAGGCG GAGGCCAGAG GGTAAAGCAG 
ATAGTGGACT ATCCACGGTG CCGCGGCCTG AAAGAGGGGG ACATTCTAAT GGAGGTCAAC 
AAGAGAAATG TCCAGAATAT GAGTCACAAT CAGGTGGTGG ATCTGCTGAG CAAATGTCCA 
AGAGGCAGTG AGGTCACCAT GTTGGTGCAA AGAGGAGTGG TGCCAGCCAA GAGGAGCCCA 
AAGCTGGTGC ACCAACTGGA GAGAAAAGAC AGTCAGAACA GCTCCCAGCA CAGTGTGTGC 
AGCCATCGCA GCACCCACAC CGATTCTCCC AGCCACCCAC CCTCTGCCAT GCCCAGTGAG 
GCAGTGGTCC CCACTCCCGC TGCCCCCACT CAGCCTCTGC CAGTCTTGCC CCCCCAGGAC 
CCCGCAGATG GCATGCTCAC CCTCCAGAAG AAGCCAGACC CTTTTAAGAT CTGGGCACAG 
TCCAGGAGCA TGTATGAGAG TCGACTACCA GATTGCCAGG AGCAAGACAT TTTCCTTTGG 
CGGAAAGATA CAGGTTTTGG GTTTCGCATC TTGGGAGGAA ATGAGCCTGG GGAGCCTATA 
TACATAGGGC ACATAGTGAA GTACGGGGCC GCAGATGAAG ATGGCCGCCT GCGATCGGGT 
GACGAGCTCA TCTGTGTGGA CGGCACGGCT GTAGTGGGCA AATCTCACCA GCTGGTGGTG 
CAGCTGATGC AGCAGGCAGC CAAGCAGGGC CACGTCAACC TCACTGTCAG ACGCAAGACC 
CCCGGATACG GAGGGTCAAA AGGAGATGGT GACGTTCCTC CATCCCCAGC CTCCTCCCAC 
CACAGCAGCA CCCAGGCACC CAGTCTGACG GAGGGAAAGC GCACCCCTCA GGGCAGCCAG 
AACTCACTCA ACACTGTCAG CTCCGGCAGC GGATCCACCA GCGGCATAGG CAGCGGCGGT 
GGAGGAGGCA GTGGGAGCGC GGTGGTGCCT GCCTCCCTGC AGCCTTACGA CGTGGAGATC 
CAGCGAGGAG AGAACGAGGG ATTTGGTTTT GTCATCGTGT CGTCCGTTTC CAGGCCTGAT 
GCTGGCACTA CTTTCGCTGG AAATGCTTGT GTGGCAATGC CCCACAAAAT AGGACGCATC 
ATCGAGGGCA GCCCGGCGGA CCGCTGCGGG AAGCTGAAGG TCGGGGACAG AATACTGGCT 
GTTAACGGCT GCTCCATCAC CAACAAGTCT CACTCAGACA TCGTCAACCT GATTAAGGAA 
GCCGGGAACA CCGTCTCACT CAGGATCATC CCAGGTGATG AATCTTCCAA TGCTTCTCTG 
CTGACTAATG CTGAGAAGAT AGCTACCATC ACCACGACAC ACACACCTCA TTCCACGGAG 
ACCAGAAATA ATTCAAAGTC AAAAGGAGCT CCTCCTCCAC CCCCTTCACA GACCCAAACA 
CAGGAAGCAG AGTTTTATTC TGTGGACCTC GAGAGGGACA GTAAAGGCTT CGGCTTCAGC 
TTAAGAGGAG GACGGGAGTA CAACATGGAC CTGTACGTGT TGAGACTAGC AGAGGATGGG 
GCTGCTGTCA GAAATGGCAA GATGAGGGTG GGAGACGAGA TCCTGGAGAT TAATGGTGAG 
AGCACAAAGA GCATGAAGCA TTCACGAGCC ATTGAACTGA TCAAGAACGG CGGTCGGAGG 
GCACGACTTG TCCTAAAAAG GGGCGATGGA TCTGTACCTG AATATGCGAT GATGGCTCCT 
CATCTCGCTG TTGGTACAAT GAGGAATGAC AAGATGGGAG AGCCCTGTTT CTACCTAATG 
GGCCAAAATC AGACAACGAC GGCAGCAGTG ACGGGCACCC TCCCACACCC CGGGCACAAA 
ACACTCCGGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017265014.1
CDS	791..4705
Translation	MSKVIQKKNHWITKVKECAVVRDPSGELNLELRGGAQDGEFAYIGHIREDAVLHQDGKLSEGELLLEVEGLSVSGLPLYDIYTVINCCKGPVRFKTVRQGHKLNKDLKHFLSLRFQKSSPDHELQKTIRANLYRHAVPCTTRAPRDGEVPGVDYNFLSVEDFLQLEESGTLLEIGTYEGNYYGTPKPPRQPIIGTVTLSDTGSHQSTPKRTKSYNDMQNVRVVPADDDDDDQATEMNNSFTGTLVRSLFPSPLLCTGNPNDQDESRTGILRPYPVRDNALACGLNNATTSTVESGQQTLAEPQVSPDDPLGPLPENWEMAYTENGELYFIDTAKPHHQDMAVHFQSVHNTKTTSWLDPRCRDKASRPLEECDDDEEGIHTEDLESDLELPPGWERIDDPVYGVYYVDHINRKTQYENPVVEARRQKLLEQQQQPQPPEGERYIREWIEEHSAAAAPLANYAPNHLETYRDPQVPPALPPGPAGIKRGKPFFTRNPAELKGTFINTKLKKSHRGFGFTVVGGDEPDEFLQIKSLVLDGPAAVDGKMETGDVIVSVNDTIVLGYTHAQVVKIFQSIPIGSMVDLALCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETFDTPPNQAGPLNSMREPRPLSPSAEVASNGSHGYSSDVVTLASSIATQPELITIHMEKGDKGFGFTIADSLTGGGQRVKQIVDYPRCRGLKEGDILMEVNKRNVQNMSHNQVVDLLSKCPRGSEVTMLVQRGVVPAKRSPKLVHQLERKDSQNSSQHSVCSHRSTHTDSPSHPPSAMPSEAVVPTPAAPTQPLPVLPPQDPADGMLTLQKKPDPFKIWAQSRSMYESRLPDCQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTPGYGGSKGDGDVPPSPASSHHSSTQAPSLTEGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGSAVVPASLQPYDVEIQRGENEGFGFVIVSSVSRPDAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVSLRIIPGDESSNASLLTNAEKIATITTTHTPHSTETRNNSKSKGAPPPPPSQTQTQEAEFYSVDLERDSKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKSMKHSRAIELIKNGGRRARLVLKRGDGSVPEYAMMAPHLAVGTMRNDKMGEPCFYLMGQNQTTTAAVTGTLPHPGHKTLRK

Target ORF information:

RefSeq Version	XM_017409525.1
Organism	Kryptolebias marmoratus(mangrove rivulus)
Definition	Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017409525.1

ORF Insert Sequence:

ATGTCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACGA AAGTGAAGGA GTGCGCCGTG 
GTTCGGGACC CCAGCGGGGA GCTGAACTTG GAGCTGCGCG GCGGGGCGCA GGACGGAGAG 
TTCGCCTACA TCGGACACAT CAGGGAGGAT GCTGTGCTCC ACCAGGACGG GAAACTGTCT 
GAGGGAGAGC TGCTGCTGGA GGTGGAGGGG CTGTCCGTGT CTGGATTACC TCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAAGGT CCCGTCAGGT TCAAAACTGT CCGCCAAGGT 
CACAAGCTCA ACAAGGACCT AAAGCACTTC CTGAGCTTGA GGTTTCAGAA GTCATCACCT 
GACCACGAGT TGCAGAAGAC TATCCGAGCC AATCTATATC GTCATGCCGT GCCTTGTACA 
ACCCGCGCCC CTCGAGACGG AGAGGTGCCA GGGGTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTTGAGGA GAGTGGCACA CTACTGGAAA TAGGAACGTA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC GCCGAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
ACGGGCTCCC ATCAGTCCAC CCCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAACGTC 
CGCGTAGTGC CTGCTGACGA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGAACCT TAGTGCGTAG CCTTTTCCCC TCTCCTTTGT TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGTAC CGGCATCCTT CGGCCATATC CTGTGCGTGA TAATGCTCTG 
GCCTGTGGTC TGAACAATGC CACCACCTCC ACTGTTGAAA GTGGGCAGCA GACGCTCGCA 
GAACCACAGG TCTCTCCAGA TGACCCTCTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACTGAGA ACGGGGAGCT GTACTTCATA GACACTGCAA AGCCTCACCA CCAGGACATG 
GCTGTGCATT TTCAGAGTGT CCACAACACA AAGACCACAT CATGGCTGGA TCCGCGGTGC 
CGAGACAAAG CCTCCAGGCC TTTGGAAGAG TGTGATGATG ATGAAGAAGG GATACATACG 
GAAGACCTGG AAAGTGATCT AGAGCTGCCC CCAGGATGGG AAAGAATAGA TGACCCCGTG 
TACGGAGTGT ACTATGTAGA TCATATAAAC AGGAAGACCC AATATGAGAA CCCAGTGGTG 
GAGGCGCGGC GCCAAAAACT CTTGGAGCAG CAGCAACAGC CGCAGCCTCC AGAAGGTGAG 
CGGTATATTC GAGAGTGGAT AGAGGAGCAC TCAGCTGCAG CAGCTCCTTT GGCAAACTAT 
GCTCCAAACC ACCTGGAGAC CTACAGGGAC CCACAAGTCC CTCCAGCTCT ACCTCCAGGC 
CCCGCTGGCA TCAAAAGAGG GAAACCTTTC TTTACGAGAA ACCCAGCAGA GCTGAAGGGA 
ACCTTCATCA ACACCAAGCT GAAGAAAAGT CACCGTGGAT TCGGGTTCAC CGTGGTGGGA 
GGTGACGAGC CCGACGAGTT CCTGCAGATC AAGAGTCTGG TGCTGGATGG GCCTGCGGCT 
GTTGATGGCA AGATGGAGAC AGGTGATGTG ATTGTCAGCG TGAATGACAC TATTGTGCTG 
GGCTACACTC ATGCTCAGGT GGTGAAGATC TTCCAGTCCA TCCCTATCGG CTCCATGGTG 
GACCTGGCCT TATGCCGTGG CTACCCACTG CCCTTTGACC CAGACGACCC AAACACCAGC 
CTGGTGACCT CTGTGGCCAT CTTGGACAAG GAACCCATTA TTGTCAATGG GCAAGAGACG 
TTTGACACGC CGCCTAACCA GGCTGGACCC CTTAACAGCA TGCGTGAGCC ACGGCCTCTC 
AGCCCCTCAG CTGAGGTGGC GTCCAACGGC TCCCATGGTT ACTCCAGCGA CGTGGTCACG 
TTGGCCTCGT CCATAGCCAC TCAGCCCGAG CTCATCACCA TCCACATGGA GAAGGGGGAC 
AAAGGTTTTG GCTTCACCAT CGCTGACAGC CTAACAGGCG GAGGCCAGAG GGTAAAGCAG 
ATAGTGGACT ATCCACGGTG CCGCGGCCTG AAAGAGGGGG ACATTCTAAT GGAGGTCAAC 
AAGAGAAATG TCCAGAATAT GAGTCACAAT CAGGTGGTGG ATCTGCTGAG CAAATGTCCA 
AGAGGCAGTG AGGTCACCAT GTTGGTGCAA AGAGGAGTGG TGCCAGCCAA GAGGAGCCCA 
AAGCTGGTGC ACCAACTGGA GAGAAAAGAC AGTCAGAACA GCTCCCAGCA CAGTGTGTGC 
AGCCATCGCA GCACCCACAC CGATTCTCCC AGCCACCCAC CCTCTGCCAT GCCCAGTGAG 
GCAGTGGTCC CCACTCCCGC TGCCCCCACT CAGCCTCTGC CAGTCTTGCC CCCCCAGGAC 
CCCGCAGATG GCATGCTCAC CCTCCAGAAG AAGCCAGACC CTTTTAAGAT CTGGGCACAG 
TCCAGGAGCA TGTATGAGAG TCGACTACCA GATTGCCAGG AGCAAGACAT TTTCCTTTGG 
CGGAAAGATA CAGGTTTTGG GTTTCGCATC TTGGGAGGAA ATGAGCCTGG GGAGCCTATA 
TACATAGGGC ACATAGTGAA GTACGGGGCC GCAGATGAAG ATGGCCGCCT GCGATCGGGT 
GACGAGCTCA TCTGTGTGGA CGGCACGGCT GTAGTGGGCA AATCTCACCA GCTGGTGGTG 
CAGCTGATGC AGCAGGCAGC CAAGCAGGGC CACGTCAACC TCACTGTCAG ACGCAAGACC 
CCCGGATACG GAGGGTCAAA AGGAGATGGT GACGTTCCTC CATCCCCAGC CTCCTCCCAC 
CACAGCAGCA CCCAGGCACC CAGTCTGACG GAGGGAAAGC GCACCCCTCA GGGCAGCCAG 
AACTCACTCA ACACTGTCAG CTCCGGCAGC GGATCCACCA GCGGCATAGG CAGCGGCGGT 
GGAGGAGGCA GTGGGAGCGC GGTGGTGCCT GCCTCCCTGC AGCCTTACGA CGTGGAGATC 
CAGCGAGGAG AGAACGAGGG ATTTGGTTTT GTCATCGTGT CGTCCGTTTC CAGGCCTGAT 
GCTGGCACTA CTTTCGCTGG AAATGCTTGT GTGGCAATGC CCCACAAAAT AGGACGCATC 
ATCGAGGGCA GCCCGGCGGA CCGCTGCGGG AAGCTGAAGG TCGGGGACAG AATACTGGCT 
GTTAACGGCT GCTCCATCAC CAACAAGTCT CACTCAGACA TCGTCAACCT GATTAAGGAA 
GCCGGGAACA CCGTCTCACT CAGGATCATC CCAGGTGATG AATCTTCCAA TGCTTCTCTG 
CTGACTAATG CTGAGAAGAT AGCTACCATC ACCACGACAC ACACACCTCA TTCCACGGAG 
ACCAGAAATA ATTCAAAGTC AAAAGGAGCT CCTCCTCCAC CCCCTTCACA GACCCAAACA 
CAGGAAGCAG AGTTTTATTC TGTGGACCTC GAGAGGGACA GTAAAGGCTT CGGCTTCAGC 
TTAAGAGGAG GACGGGAGTA CAACATGGAC CTGTACGTGT TGAGACTAGC AGAGGATGGG 
GCTGCTGTCA GAAATGGCAA GATGAGGGTG GGAGACGAGA TCCTGGAGAT TAATGGTGAG 
AGCACAAAGA GCATGAAGCA TTCACGAGCC ATTGAACTGA TCAAGAACGG CGGTCGGAGG 
GCACGACTTG TCCTAAAAAG GGGCGATGGA TCTGTACCTG AATATGCGAT GATGGCTCCT 
CATCTCGCTG TTGGTACAAT GAGGAATGAC AAGATGGGAG AGCCCTGTTT CTACCTAATG 
GGCCAAAATC AGACAACGAC GGCAGCAGTG ACGGGCACCC TCCCACACCC CGGGCACAAA 
ACACTCCGGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108232031 ( XM_017409525.2 ) cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus) -> XP_017265014.1 Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X9, mRNA.

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CloneID	OKr06775
Clone ID Related Accession (Same CDS sequence)	XM_017409525.1 , XM_017409525.2
Accession Version	XM_017409525.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-03
Organism	Kryptolebias marmoratus(mangrove rivulus)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016094253.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 4, 2018 this sequence version replaced XM_017409525.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Kryptolebias marmoratus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACGA AAGTGAAGGA GTGCGCCGTG 
GTTCGGGACC CCAGCGGGGA GCTGAACTTG GAGCTGCGCG GCGGGGCGCA GGACGGAGAG 
TTCGCCTACA TCGGACACAT CAGGGAGGAT GCTGTGCTCC ACCAGGACGG GAAACTGTCT 
GAGGGAGAGC TGCTGCTGGA GGTGGAGGGG CTGTCCGTGT CTGGATTACC TCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAAGGT CCCGTCAGGT TCAAAACTGT CCGCCAAGGT 
CACAAGCTCA ACAAGGACCT AAAGCACTTC CTGAGCTTGA GGTTTCAGAA GTCATCACCT 
GACCACGAGT TGCAGAAGAC TATCCGAGCC AATCTATATC GTCATGCCGT GCCTTGTACA 
ACCCGCGCCC CTCGAGACGG AGAGGTGCCA GGGGTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTTGAGGA GAGTGGCACA CTACTGGAAA TAGGAACGTA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC GCCGAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
ACGGGCTCCC ATCAGTCCAC CCCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAACGTC 
CGCGTAGTGC CTGCTGACGA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGAACCT TAGTGCGTAG CCTTTTCCCC TCTCCTTTGT TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGTAC CGGCATCCTT CGGCCATATC CTGTGCGTGA TAATGCTCTG 
GCCTGTGGTC TGAACAATGC CACCACCTCC ACTGTTGAAA GTGGGCAGCA GACGCTCGCA 
GAACCACAGG TCTCTCCAGA TGACCCTCTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACTGAGA ACGGGGAGCT GTACTTCATA GACACTGCAA AGCCTCACCA CCAGGACATG 
GCTGTGCATT TTCAGAGTGT CCACAACACA AAGACCACAT CATGGCTGGA TCCGCGGTGC 
CGAGACAAAG CCTCCAGGCC TTTGGAAGAG TGTGATGATG ATGAAGAAGG GATACATACG 
GAAGACCTGG AAAGTGATCT AGAGCTGCCC CCAGGATGGG AAAGAATAGA TGACCCCGTG 
TACGGAGTGT ACTATGTAGA TCATATAAAC AGGAAGACCC AATATGAGAA CCCAGTGGTG 
GAGGCGCGGC GCCAAAAACT CTTGGAGCAG CAGCAACAGC CGCAGCCTCC AGAAGGTGAG 
CGGTATATTC GAGAGTGGAT AGAGGAGCAC TCAGCTGCAG CAGCTCCTTT GGCAAACTAT 
GCTCCAAACC ACCTGGAGAC CTACAGGGAC CCACAAGTCC CTCCAGCTCT ACCTCCAGGC 
CCCGCTGGCA TCAAAAGAGG GAAACCTTTC TTTACGAGAA ACCCAGCAGA GCTGAAGGGA 
ACCTTCATCA ACACCAAGCT GAAGAAAAGT CACCGTGGAT TCGGGTTCAC CGTGGTGGGA 
GGTGACGAGC CCGACGAGTT CCTGCAGATC AAGAGTCTGG TGCTGGATGG GCCTGCGGCT 
GTTGATGGCA AGATGGAGAC AGGTGATGTG ATTGTCAGCG TGAATGACAC TATTGTGCTG 
GGCTACACTC ATGCTCAGGT GGTGAAGATC TTCCAGTCCA TCCCTATCGG CTCCATGGTG 
GACCTGGCCT TATGCCGTGG CTACCCACTG CCCTTTGACC CAGACGACCC AAACACCAGC 
CTGGTGACCT CTGTGGCCAT CTTGGACAAG GAACCCATTA TTGTCAATGG GCAAGAGACG 
TTTGACACGC CGCCTAACCA GGCTGGACCC CTTAACAGCA TGCGTGAGCC ACGGCCTCTC 
AGCCCCTCAG CTGAGGTGGC GTCCAACGGC TCCCATGGTT ACTCCAGCGA CGTGGTCACG 
TTGGCCTCGT CCATAGCCAC TCAGCCCGAG CTCATCACCA TCCACATGGA GAAGGGGGAC 
AAAGGTTTTG GCTTCACCAT CGCTGACAGC CTAACAGGCG GAGGCCAGAG GGTAAAGCAG 
ATAGTGGACT ATCCACGGTG CCGCGGCCTG AAAGAGGGGG ACATTCTAAT GGAGGTCAAC 
AAGAGAAATG TCCAGAATAT GAGTCACAAT CAGGTGGTGG ATCTGCTGAG CAAATGTCCA 
AGAGGCAGTG AGGTCACCAT GTTGGTGCAA AGAGGAGTGG TGCCAGCCAA GAGGAGCCCA 
AAGCTGGTGC ACCAACTGGA GAGAAAAGAC AGTCAGAACA GCTCCCAGCA CAGTGTGTGC 
AGCCATCGCA GCACCCACAC CGATTCTCCC AGCCACCCAC CCTCTGCCAT GCCCAGTGAG 
GCAGTGGTCC CCACTCCCGC TGCCCCCACT CAGCCTCTGC CAGTCTTGCC CCCCCAGGAC 
CCCGCAGATG GCATGCTCAC CCTCCAGAAG AAGCCAGACC CTTTTAAGAT CTGGGCACAG 
TCCAGGAGCA TGTATGAGAG TCGACTACCA GATTGCCAGG AGCAAGACAT TTTCCTTTGG 
CGGAAAGATA CAGGTTTTGG GTTTCGCATC TTGGGAGGAA ATGAGCCTGG GGAGCCTATA 
TACATAGGGC ACATAGTGAA GTACGGGGCC GCAGATGAAG ATGGCCGCCT GCGATCGGGT 
GACGAGCTCA TCTGTGTGGA CGGCACGGCT GTAGTGGGCA AATCTCACCA GCTGGTGGTG 
CAGCTGATGC AGCAGGCAGC CAAGCAGGGC CACGTCAACC TCACTGTCAG ACGCAAGACC 
CCCGGATACG GAGGGTCAAA AGGAGATGGT GACGTTCCTC CATCCCCAGC CTCCTCCCAC 
CACAGCAGCA CCCAGGCACC CAGTCTGACG GAGGGAAAGC GCACCCCTCA GGGCAGCCAG 
AACTCACTCA ACACTGTCAG CTCCGGCAGC GGATCCACCA GCGGCATAGG CAGCGGCGGT 
GGAGGAGGCA GTGGGAGCGC GGTGGTGCCT GCCTCCCTGC AGCCTTACGA CGTGGAGATC 
CAGCGAGGAG AGAACGAGGG ATTTGGTTTT GTCATCGTGT CGTCCGTTTC CAGGCCTGAT 
GCTGGCACTA CTTTCGCTGG AAATGCTTGT GTGGCAATGC CCCACAAAAT AGGACGCATC 
ATCGAGGGCA GCCCGGCGGA CCGCTGCGGG AAGCTGAAGG TCGGGGACAG AATACTGGCT 
GTTAACGGCT GCTCCATCAC CAACAAGTCT CACTCAGACA TCGTCAACCT GATTAAGGAA 
GCCGGGAACA CCGTCTCACT CAGGATCATC CCAGGTGATG AATCTTCCAA TGCTTCTCTG 
CTGACTAATG CTGAGAAGAT AGCTACCATC ACCACGACAC ACACACCTCA TTCCACGGAG 
ACCAGAAATA ATTCAAAGTC AAAAGGAGCT CCTCCTCCAC CCCCTTCACA GACCCAAACA 
CAGGAAGCAG AGTTTTATTC TGTGGACCTC GAGAGGGACA GTAAAGGCTT CGGCTTCAGC 
TTAAGAGGAG GACGGGAGTA CAACATGGAC CTGTACGTGT TGAGACTAGC AGAGGATGGG 
GCTGCTGTCA GAAATGGCAA GATGAGGGTG GGAGACGAGA TCCTGGAGAT TAATGGTGAG 
AGCACAAAGA GCATGAAGCA TTCACGAGCC ATTGAACTGA TCAAGAACGG CGGTCGGAGG 
GCACGACTTG TCCTAAAAAG GGGCGATGGA TCTGTACCTG AATATGCGAT GATGGCTCCT 
CATCTCGCTG TTGGTACAAT GAGGAATGAC AAGATGGGAG AGCCCTGTTT CTACCTAATG 
GGCCAAAATC AGACAACGAC GGCAGCAGTG ACGGGCACCC TCCCACACCC CGGGCACAAA 
ACACTCCGGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017265014.1
CDS	917..4831
Translation	MSKVIQKKNHWITKVKECAVVRDPSGELNLELRGGAQDGEFAYIGHIREDAVLHQDGKLSEGELLLEVEGLSVSGLPLYDIYTVINCCKGPVRFKTVRQGHKLNKDLKHFLSLRFQKSSPDHELQKTIRANLYRHAVPCTTRAPRDGEVPGVDYNFLSVEDFLQLEESGTLLEIGTYEGNYYGTPKPPRQPIIGTVTLSDTGSHQSTPKRTKSYNDMQNVRVVPADDDDDDQATEMNNSFTGTLVRSLFPSPLLCTGNPNDQDESRTGILRPYPVRDNALACGLNNATTSTVESGQQTLAEPQVSPDDPLGPLPENWEMAYTENGELYFIDTAKPHHQDMAVHFQSVHNTKTTSWLDPRCRDKASRPLEECDDDEEGIHTEDLESDLELPPGWERIDDPVYGVYYVDHINRKTQYENPVVEARRQKLLEQQQQPQPPEGERYIREWIEEHSAAAAPLANYAPNHLETYRDPQVPPALPPGPAGIKRGKPFFTRNPAELKGTFINTKLKKSHRGFGFTVVGGDEPDEFLQIKSLVLDGPAAVDGKMETGDVIVSVNDTIVLGYTHAQVVKIFQSIPIGSMVDLALCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETFDTPPNQAGPLNSMREPRPLSPSAEVASNGSHGYSSDVVTLASSIATQPELITIHMEKGDKGFGFTIADSLTGGGQRVKQIVDYPRCRGLKEGDILMEVNKRNVQNMSHNQVVDLLSKCPRGSEVTMLVQRGVVPAKRSPKLVHQLERKDSQNSSQHSVCSHRSTHTDSPSHPPSAMPSEAVVPTPAAPTQPLPVLPPQDPADGMLTLQKKPDPFKIWAQSRSMYESRLPDCQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTPGYGGSKGDGDVPPSPASSHHSSTQAPSLTEGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGSAVVPASLQPYDVEIQRGENEGFGFVIVSSVSRPDAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVSLRIIPGDESSNASLLTNAEKIATITTTHTPHSTETRNNSKSKGAPPPPPSQTQTQEAEFYSVDLERDSKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKSMKHSRAIELIKNGGRRARLVLKRGDGSVPEYAMMAPHLAVGTMRNDKMGEPCFYLMGQNQTTTAAVTGTLPHPGHKTLRK

Target ORF information:

RefSeq Version	XM_017409525.2
Organism	Kryptolebias marmoratus(mangrove rivulus)
Definition	Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017409525.2

ORF Insert Sequence:

ATGTCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACGA AAGTGAAGGA GTGCGCCGTG 
GTTCGGGACC CCAGCGGGGA GCTGAACTTG GAGCTGCGCG GCGGGGCGCA GGACGGAGAG 
TTCGCCTACA TCGGACACAT CAGGGAGGAT GCTGTGCTCC ACCAGGACGG GAAACTGTCT 
GAGGGAGAGC TGCTGCTGGA GGTGGAGGGG CTGTCCGTGT CTGGATTACC TCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAAGGT CCCGTCAGGT TCAAAACTGT CCGCCAAGGT 
CACAAGCTCA ACAAGGACCT AAAGCACTTC CTGAGCTTGA GGTTTCAGAA GTCATCACCT 
GACCACGAGT TGCAGAAGAC TATCCGAGCC AATCTATATC GTCATGCCGT GCCTTGTACA 
ACCCGCGCCC CTCGAGACGG AGAGGTGCCA GGGGTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTTGAGGA GAGTGGCACA CTACTGGAAA TAGGAACGTA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC GCCGAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
ACGGGCTCCC ATCAGTCCAC CCCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAACGTC 
CGCGTAGTGC CTGCTGACGA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGAACCT TAGTGCGTAG CCTTTTCCCC TCTCCTTTGT TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGTAC CGGCATCCTT CGGCCATATC CTGTGCGTGA TAATGCTCTG 
GCCTGTGGTC TGAACAATGC CACCACCTCC ACTGTTGAAA GTGGGCAGCA GACGCTCGCA 
GAACCACAGG TCTCTCCAGA TGACCCTCTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACTGAGA ACGGGGAGCT GTACTTCATA GACACTGCAA AGCCTCACCA CCAGGACATG 
GCTGTGCATT TTCAGAGTGT CCACAACACA AAGACCACAT CATGGCTGGA TCCGCGGTGC 
CGAGACAAAG CCTCCAGGCC TTTGGAAGAG TGTGATGATG ATGAAGAAGG GATACATACG 
GAAGACCTGG AAAGTGATCT AGAGCTGCCC CCAGGATGGG AAAGAATAGA TGACCCCGTG 
TACGGAGTGT ACTATGTAGA TCATATAAAC AGGAAGACCC AATATGAGAA CCCAGTGGTG 
GAGGCGCGGC GCCAAAAACT CTTGGAGCAG CAGCAACAGC CGCAGCCTCC AGAAGGTGAG 
CGGTATATTC GAGAGTGGAT AGAGGAGCAC TCAGCTGCAG CAGCTCCTTT GGCAAACTAT 
GCTCCAAACC ACCTGGAGAC CTACAGGGAC CCACAAGTCC CTCCAGCTCT ACCTCCAGGC 
CCCGCTGGCA TCAAAAGAGG GAAACCTTTC TTTACGAGAA ACCCAGCAGA GCTGAAGGGA 
ACCTTCATCA ACACCAAGCT GAAGAAAAGT CACCGTGGAT TCGGGTTCAC CGTGGTGGGA 
GGTGACGAGC CCGACGAGTT CCTGCAGATC AAGAGTCTGG TGCTGGATGG GCCTGCGGCT 
GTTGATGGCA AGATGGAGAC AGGTGATGTG ATTGTCAGCG TGAATGACAC TATTGTGCTG 
GGCTACACTC ATGCTCAGGT GGTGAAGATC TTCCAGTCCA TCCCTATCGG CTCCATGGTG 
GACCTGGCCT TATGCCGTGG CTACCCACTG CCCTTTGACC CAGACGACCC AAACACCAGC 
CTGGTGACCT CTGTGGCCAT CTTGGACAAG GAACCCATTA TTGTCAATGG GCAAGAGACG 
TTTGACACGC CGCCTAACCA GGCTGGACCC CTTAACAGCA TGCGTGAGCC ACGGCCTCTC 
AGCCCCTCAG CTGAGGTGGC GTCCAACGGC TCCCATGGTT ACTCCAGCGA CGTGGTCACG 
TTGGCCTCGT CCATAGCCAC TCAGCCCGAG CTCATCACCA TCCACATGGA GAAGGGGGAC 
AAAGGTTTTG GCTTCACCAT CGCTGACAGC CTAACAGGCG GAGGCCAGAG GGTAAAGCAG 
ATAGTGGACT ATCCACGGTG CCGCGGCCTG AAAGAGGGGG ACATTCTAAT GGAGGTCAAC 
AAGAGAAATG TCCAGAATAT GAGTCACAAT CAGGTGGTGG ATCTGCTGAG CAAATGTCCA 
AGAGGCAGTG AGGTCACCAT GTTGGTGCAA AGAGGAGTGG TGCCAGCCAA GAGGAGCCCA 
AAGCTGGTGC ACCAACTGGA GAGAAAAGAC AGTCAGAACA GCTCCCAGCA CAGTGTGTGC 
AGCCATCGCA GCACCCACAC CGATTCTCCC AGCCACCCAC CCTCTGCCAT GCCCAGTGAG 
GCAGTGGTCC CCACTCCCGC TGCCCCCACT CAGCCTCTGC CAGTCTTGCC CCCCCAGGAC 
CCCGCAGATG GCATGCTCAC CCTCCAGAAG AAGCCAGACC CTTTTAAGAT CTGGGCACAG 
TCCAGGAGCA TGTATGAGAG TCGACTACCA GATTGCCAGG AGCAAGACAT TTTCCTTTGG 
CGGAAAGATA CAGGTTTTGG GTTTCGCATC TTGGGAGGAA ATGAGCCTGG GGAGCCTATA 
TACATAGGGC ACATAGTGAA GTACGGGGCC GCAGATGAAG ATGGCCGCCT GCGATCGGGT 
GACGAGCTCA TCTGTGTGGA CGGCACGGCT GTAGTGGGCA AATCTCACCA GCTGGTGGTG 
CAGCTGATGC AGCAGGCAGC CAAGCAGGGC CACGTCAACC TCACTGTCAG ACGCAAGACC 
CCCGGATACG GAGGGTCAAA AGGAGATGGT GACGTTCCTC CATCCCCAGC CTCCTCCCAC 
CACAGCAGCA CCCAGGCACC CAGTCTGACG GAGGGAAAGC GCACCCCTCA GGGCAGCCAG 
AACTCACTCA ACACTGTCAG CTCCGGCAGC GGATCCACCA GCGGCATAGG CAGCGGCGGT 
GGAGGAGGCA GTGGGAGCGC GGTGGTGCCT GCCTCCCTGC AGCCTTACGA CGTGGAGATC 
CAGCGAGGAG AGAACGAGGG ATTTGGTTTT GTCATCGTGT CGTCCGTTTC CAGGCCTGAT 
GCTGGCACTA CTTTCGCTGG AAATGCTTGT GTGGCAATGC CCCACAAAAT AGGACGCATC 
ATCGAGGGCA GCCCGGCGGA CCGCTGCGGG AAGCTGAAGG TCGGGGACAG AATACTGGCT 
GTTAACGGCT GCTCCATCAC CAACAAGTCT CACTCAGACA TCGTCAACCT GATTAAGGAA 
GCCGGGAACA CCGTCTCACT CAGGATCATC CCAGGTGATG AATCTTCCAA TGCTTCTCTG 
CTGACTAATG CTGAGAAGAT AGCTACCATC ACCACGACAC ACACACCTCA TTCCACGGAG 
ACCAGAAATA ATTCAAAGTC AAAAGGAGCT CCTCCTCCAC CCCCTTCACA GACCCAAACA 
CAGGAAGCAG AGTTTTATTC TGTGGACCTC GAGAGGGACA GTAAAGGCTT CGGCTTCAGC 
TTAAGAGGAG GACGGGAGTA CAACATGGAC CTGTACGTGT TGAGACTAGC AGAGGATGGG 
GCTGCTGTCA GAAATGGCAA GATGAGGGTG GGAGACGAGA TCCTGGAGAT TAATGGTGAG 
AGCACAAAGA GCATGAAGCA TTCACGAGCC ATTGAACTGA TCAAGAACGG CGGTCGGAGG 
GCACGACTTG TCCTAAAAAG GGGCGATGGA TCTGTACCTG AATATGCGAT GATGGCTCCT 
CATCTCGCTG TTGGTACAAT GAGGAATGAC AAGATGGGAG AGCCCTGTTT CTACCTAATG 
GGCCAAAATC AGACAACGAC GGCAGCAGTG ACGGGCACCC TCCCACACCC CGGGCACAAA 
ACACTCCGGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108232031 ( XM_017409526.1 ) cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus) -> XP_017265015.1 Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X9, mRNA.

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CloneID	OKr06776
Clone ID Related Accession (Same CDS sequence)	XM_017409526.1
Accession Version	XM_017409526.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-27
Organism	Kryptolebias marmoratus(mangrove rivulus)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016094253.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Kryptolebias marmoratus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACGA AAGTGAAGGA GTGCGCCGTG 
GTTCGGGACC CCAGCGGGGA GCTGAACTTG GAGCTGCGCG GCGGGGCGCA GGACGGAGAG 
TTCGCCTACA TCGGACACAT CAGGGAGGAT GCTGTGCTCC ACCAGGACGG GAAACTGTCT 
GAGGGAGAGC TGCTGCTGGA GGTGGAGGGG CTGTCCGTGT CTGGATTACC TCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAAGGT CCCGTCAGGT TCAAAACTGT CCGCCAAGGT 
CACAAGCTCA ACAAGGACCT AAAGCACTTC CTGAGCTTGA GGTTTCAGAA GTCATCACCT 
GACCACGAGT TGCAGAAGAC TATCCGAGCC AATCTATATC GTCATGCCGT GCCTTGTACA 
ACCCGCGCCC CTCGAGACGG AGAGGTGCCA GGGGTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTTGAGGA GAGTGGCACA CTACTGGAAA TAGGAACGTA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC GCCGAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
ACGGGCTCCC ATCAGTCCAC CCCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAACGTC 
CGCGTAGTGC CTGCTGACGA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGTAACC CTAACGACCA GGACGAGAGC CGTACCGGCA TCCTTCGGCC ATATCCTGTG 
CGTGATAATG CTCTGGCCTG TGGTCTGAAC AATGCCACCA CCTCCACTGT TGAAAGTGGG 
CAGCAGACGC TCGCAGAACC ACAGGTCTCT CCAGATGACC CTCTGGGACC GCTGCCTGAA 
AACTGGGAGA TGGCCTACAC TGAGAACGGG GAGCTGTACT TCATAGACCA CAACACAAAG 
ACCACATCAT GGCTGGATCC GCGGTGCCGA GACAAAGCCT CCAGGCCTTT GGAAGAGTGT 
GATGATGATG AAGGGATACA TACGGAAGAC CTGGAAAGTG ATCTAGAGCT GCCCCCAGGA 
TGGGAAAGAA TAGATGACCC CGTGTACGGA GTGTACTATG TAGATCATAT AAACAGGAAG 
ACCCAATATG AGAACCCAGT GGTGGAGGCG CGGCGCCAAA AACTCTTGGA GCAGCAGCAA 
CAGCCGCAGC CTCCAGAAGG TGAGCGGTAT ATTCGAGAGT GGATAGAGGA GCACTCAGCT 
GCAGCAGCTC CTTTGGCAAA CTATGCTCCA AACCACCTGG AGACCTACAG GGACCCACAA 
GTCCCTCCAG CTCTACCTCC AGGCCCCGCT GGCATCAAAA GAGGGAAACC TTTCTTTACG 
AGAAACCCAG CAGAGCTGAA GGGAACCTTC ATCAACACCA AGCTGAAGAA AAGTCACCGT 
GGATTCGGGT TCACCGTGGT GGGAGGTGAC GAGCCCGACG AGTTCCTGCA GATCAAGAGT 
CTGGTGCTGG ATGGGCCTGC GGCTGTTGAT GGCAAGATGG AGACAGGTGA TGTGATTGTC 
AGCGTGAATG ACACTATTGT GCTGGGCTAC ACTCATGCTC AGGTGGTGAA GATCTTCCAG 
TCCATCCCTA TCGGCTCCAT GGTGGACCTG GCCTTATGCC GTGGCTACCC ACTGCCCTTT 
GACCCAGACG ACCCAAACAC CAGCCTGGTG ACCTCTGTGG CCATCTTGGA CAAGGAACCC 
ATTATTGTCA ATGGGCAAGA GACGTTTGAC ACGCCGCCTA ACCAGGCTGG ACCCCTTAAC 
AGCATGCGTG AGCCACGGCC TCTCAGCCCC TCAGCTGAGG TGGCGTCCAA CGGCTCCCAT 
GGTTACTCCA GCGACGTGGT CACGTTGGCC TCGTCCATAG CCACTCAGCC CGAGCTCATC 
ACCATCCACA TGGAGAAGGG GGACAAAGGT TTTGGCTTCA CCATCGCTGA CAGCCTAACA 
GGCGGAGGCC AGAGGGTAAA GCAGATAGTG GACTATCCAC GGTGCCGCGG CCTGAAAGAG 
GGGGACATTC TAATGGAGGT CAACAAGAGA AATGTCCAGA ATATGAGTCA CAATCAGGTG 
GTGGATCTGC TGAGCAAATG TCCAAGAGGC AGTGAGGTCA CCATGTTGGT GCAAAGAGGA 
GTGGTGCCAG CCAAGAGGAG CCCAAAGCTG GTGCACCAAC TGGAGAGAAA AGACAGTCAG 
AACAGCTCCC AGCACAGTGT GTGCAGCCAT CGCAGCACCC ACACCGATTC TCCCAGCCAC 
CCACCCTCTG CCATGCCCAG TGAGGCAGTG GTCCCCACTC CCGCTGCCCC CACTCAGCCT 
CTGCCAGTCT TGCCCCCCCA GGACCCCGCA GATGGCATGC TCACCCTCCA GAAGAAGCCA 
GACCCTTTTA AGATCTGGGC ACAGTCCAGG AGCATGTATG AGAGTCGACT ACCAGATTGC 
CAGGAGCAAG ACATTTTCCT TTGGCGGAAA GATACAGGTT TTGGGTTTCG CATCTTGGGA 
GGAAATGAGC CTGGGGAGCC TATATACATA GGGCACATAG TGAAGTACGG GGCCGCAGAT 
GAAGATGGCC GCCTGCGATC GGGTGACGAG CTCATCTGTG TGGACGGCAC GGCTGTAGTG 
GGCAAATCTC ACCAGCTGGT GGTGCAGCTG ATGCAGCAGG CAGCCAAGCA GGGCCACGTC 
AACCTCACTG TCAGACGCAA GACCCCCGGA TACGGAGGGT CAAAAGGAGA TGGTGACGTT 
CCTCCATCCC CAGCCTCCTC CCACCACAGC AGCACCCAGG CACCCAGTCT GACGGAGGGA 
AAGCGCACCC CTCAGGGCAG CCAGAACTCA CTCAACACTG TCAGCTCCGG CAGCGGATCC 
ACCAGCGGCA TAGGCAGCGG CGGTGGAGGA GGCAGTGGGA GCGCGGTGGT GCCTGCCTCC 
CTGCAGCCTT ACGACGTGGA GATCCAGCGA GGAGAGAACG AGGGATTTGG TTTTGTCATC 
GTGTCGTCCG TTTCCAGGCC TGATGCTGGC ACTACTTTCG CTGGAAATGC TTGTGTGGCA 
ATGCCCCACA AAATAGGACG CATCATCGAG GGCAGCCCGG CGGACCGCTG CGGGAAGCTG 
AAGGTCGGGG ACAGAATACT GGCTGTTAAC GGCTGCTCCA TCACCAACAA GTCTCACTCA 
GACATCGTCA ACCTGATTAA GGAAGCCGGG AACACCGTCT CACTCAGGAT CATCCCAGGT 
GATGAATCTT CCAATGCTTC TCTGCTGACT AATGCTGAGA AGATAGCTAC CATCACCACG 
ACACACACAC CTCATTCCAC GGAGACCAGA AATAATTCAA AGTCAAAAGG AGCTCCTCCT 
CCACCCCCTT CACAGACCCA AACACAGGAA GCAGAGTTTT ATTCTGTGGA CCTCGAGAGG 
GACAGTAAAG GCTTCGGCTT CAGCTTAAGA GGAGGACGGG AGTACAACAT GGACCTGTAC 
GTGTTGAGAC TAGCAGAGGA TGGGGCTGCT GTCAGAAATG GCAAGATGAG GGTGGGAGAC 
GAGATCCTGG AGATTAATGG TGAGAGCACA AAGAGCATGA AGCATTCACG AGCCATTGAA 
CTGATCAAGA ACGGCGGTCG GAGGGCACGA CTTGTCCTAA AAAGGGGCGA TGGATCTGTA 
CCTGAATATG CGATGATGGC TCCTCATCTC GCTGTTGGTA CAATGAGGAA TGACAAGATG 
GGAGAGCCCT GTTTCTACCT AATGGGCCAA AATCAGACAA CGACGGCAGC AGTGACGGGC 
ACCCTCCCAC ACCCCGGGCA CAAAACACTC CGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017265015.1
CDS	788..4606
Translation	MSKVIQKKNHWITKVKECAVVRDPSGELNLELRGGAQDGEFAYIGHIREDAVLHQDGKLSEGELLLEVEGLSVSGLPLYDIYTVINCCKGPVRFKTVRQGHKLNKDLKHFLSLRFQKSSPDHELQKTIRANLYRHAVPCTTRAPRDGEVPGVDYNFLSVEDFLQLEESGTLLEIGTYEGNYYGTPKPPRQPIIGTVTLSDTGSHQSTPKRTKSYNDMQNVRVVPADDDDDDQATEMNNSFTGNPNDQDESRTGILRPYPVRDNALACGLNNATTSTVESGQQTLAEPQVSPDDPLGPLPENWEMAYTENGELYFIDHNTKTTSWLDPRCRDKASRPLEECDDDEGIHTEDLESDLELPPGWERIDDPVYGVYYVDHINRKTQYENPVVEARRQKLLEQQQQPQPPEGERYIREWIEEHSAAAAPLANYAPNHLETYRDPQVPPALPPGPAGIKRGKPFFTRNPAELKGTFINTKLKKSHRGFGFTVVGGDEPDEFLQIKSLVLDGPAAVDGKMETGDVIVSVNDTIVLGYTHAQVVKIFQSIPIGSMVDLALCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETFDTPPNQAGPLNSMREPRPLSPSAEVASNGSHGYSSDVVTLASSIATQPELITIHMEKGDKGFGFTIADSLTGGGQRVKQIVDYPRCRGLKEGDILMEVNKRNVQNMSHNQVVDLLSKCPRGSEVTMLVQRGVVPAKRSPKLVHQLERKDSQNSSQHSVCSHRSTHTDSPSHPPSAMPSEAVVPTPAAPTQPLPVLPPQDPADGMLTLQKKPDPFKIWAQSRSMYESRLPDCQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTPGYGGSKGDGDVPPSPASSHHSSTQAPSLTEGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGSAVVPASLQPYDVEIQRGENEGFGFVIVSSVSRPDAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVSLRIIPGDESSNASLLTNAEKIATITTTHTPHSTETRNNSKSKGAPPPPPSQTQTQEAEFYSVDLERDSKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKSMKHSRAIELIKNGGRRARLVLKRGDGSVPEYAMMAPHLAVGTMRNDKMGEPCFYLMGQNQTTTAAVTGTLPHPGHKTLRK

Target ORF information:

RefSeq Version	XM_017409526.1
Organism	Kryptolebias marmoratus(mangrove rivulus)
Definition	Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017409526.1

ORF Insert Sequence:

ATGTCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACGA AAGTGAAGGA GTGCGCCGTG 
GTTCGGGACC CCAGCGGGGA GCTGAACTTG GAGCTGCGCG GCGGGGCGCA GGACGGAGAG 
TTCGCCTACA TCGGACACAT CAGGGAGGAT GCTGTGCTCC ACCAGGACGG GAAACTGTCT 
GAGGGAGAGC TGCTGCTGGA GGTGGAGGGG CTGTCCGTGT CTGGATTACC TCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAAGGT CCCGTCAGGT TCAAAACTGT CCGCCAAGGT 
CACAAGCTCA ACAAGGACCT AAAGCACTTC CTGAGCTTGA GGTTTCAGAA GTCATCACCT 
GACCACGAGT TGCAGAAGAC TATCCGAGCC AATCTATATC GTCATGCCGT GCCTTGTACA 
ACCCGCGCCC CTCGAGACGG AGAGGTGCCA GGGGTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTTGAGGA GAGTGGCACA CTACTGGAAA TAGGAACGTA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC GCCGAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
ACGGGCTCCC ATCAGTCCAC CCCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAACGTC 
CGCGTAGTGC CTGCTGACGA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGTAACC CTAACGACCA GGACGAGAGC CGTACCGGCA TCCTTCGGCC ATATCCTGTG 
CGTGATAATG CTCTGGCCTG TGGTCTGAAC AATGCCACCA CCTCCACTGT TGAAAGTGGG 
CAGCAGACGC TCGCAGAACC ACAGGTCTCT CCAGATGACC CTCTGGGACC GCTGCCTGAA 
AACTGGGAGA TGGCCTACAC TGAGAACGGG GAGCTGTACT TCATAGACCA CAACACAAAG 
ACCACATCAT GGCTGGATCC GCGGTGCCGA GACAAAGCCT CCAGGCCTTT GGAAGAGTGT 
GATGATGATG AAGGGATACA TACGGAAGAC CTGGAAAGTG ATCTAGAGCT GCCCCCAGGA 
TGGGAAAGAA TAGATGACCC CGTGTACGGA GTGTACTATG TAGATCATAT AAACAGGAAG 
ACCCAATATG AGAACCCAGT GGTGGAGGCG CGGCGCCAAA AACTCTTGGA GCAGCAGCAA 
CAGCCGCAGC CTCCAGAAGG TGAGCGGTAT ATTCGAGAGT GGATAGAGGA GCACTCAGCT 
GCAGCAGCTC CTTTGGCAAA CTATGCTCCA AACCACCTGG AGACCTACAG GGACCCACAA 
GTCCCTCCAG CTCTACCTCC AGGCCCCGCT GGCATCAAAA GAGGGAAACC TTTCTTTACG 
AGAAACCCAG CAGAGCTGAA GGGAACCTTC ATCAACACCA AGCTGAAGAA AAGTCACCGT 
GGATTCGGGT TCACCGTGGT GGGAGGTGAC GAGCCCGACG AGTTCCTGCA GATCAAGAGT 
CTGGTGCTGG ATGGGCCTGC GGCTGTTGAT GGCAAGATGG AGACAGGTGA TGTGATTGTC 
AGCGTGAATG ACACTATTGT GCTGGGCTAC ACTCATGCTC AGGTGGTGAA GATCTTCCAG 
TCCATCCCTA TCGGCTCCAT GGTGGACCTG GCCTTATGCC GTGGCTACCC ACTGCCCTTT 
GACCCAGACG ACCCAAACAC CAGCCTGGTG ACCTCTGTGG CCATCTTGGA CAAGGAACCC 
ATTATTGTCA ATGGGCAAGA GACGTTTGAC ACGCCGCCTA ACCAGGCTGG ACCCCTTAAC 
AGCATGCGTG AGCCACGGCC TCTCAGCCCC TCAGCTGAGG TGGCGTCCAA CGGCTCCCAT 
GGTTACTCCA GCGACGTGGT CACGTTGGCC TCGTCCATAG CCACTCAGCC CGAGCTCATC 
ACCATCCACA TGGAGAAGGG GGACAAAGGT TTTGGCTTCA CCATCGCTGA CAGCCTAACA 
GGCGGAGGCC AGAGGGTAAA GCAGATAGTG GACTATCCAC GGTGCCGCGG CCTGAAAGAG 
GGGGACATTC TAATGGAGGT CAACAAGAGA AATGTCCAGA ATATGAGTCA CAATCAGGTG 
GTGGATCTGC TGAGCAAATG TCCAAGAGGC AGTGAGGTCA CCATGTTGGT GCAAAGAGGA 
GTGGTGCCAG CCAAGAGGAG CCCAAAGCTG GTGCACCAAC TGGAGAGAAA AGACAGTCAG 
AACAGCTCCC AGCACAGTGT GTGCAGCCAT CGCAGCACCC ACACCGATTC TCCCAGCCAC 
CCACCCTCTG CCATGCCCAG TGAGGCAGTG GTCCCCACTC CCGCTGCCCC CACTCAGCCT 
CTGCCAGTCT TGCCCCCCCA GGACCCCGCA GATGGCATGC TCACCCTCCA GAAGAAGCCA 
GACCCTTTTA AGATCTGGGC ACAGTCCAGG AGCATGTATG AGAGTCGACT ACCAGATTGC 
CAGGAGCAAG ACATTTTCCT TTGGCGGAAA GATACAGGTT TTGGGTTTCG CATCTTGGGA 
GGAAATGAGC CTGGGGAGCC TATATACATA GGGCACATAG TGAAGTACGG GGCCGCAGAT 
GAAGATGGCC GCCTGCGATC GGGTGACGAG CTCATCTGTG TGGACGGCAC GGCTGTAGTG 
GGCAAATCTC ACCAGCTGGT GGTGCAGCTG ATGCAGCAGG CAGCCAAGCA GGGCCACGTC 
AACCTCACTG TCAGACGCAA GACCCCCGGA TACGGAGGGT CAAAAGGAGA TGGTGACGTT 
CCTCCATCCC CAGCCTCCTC CCACCACAGC AGCACCCAGG CACCCAGTCT GACGGAGGGA 
AAGCGCACCC CTCAGGGCAG CCAGAACTCA CTCAACACTG TCAGCTCCGG CAGCGGATCC 
ACCAGCGGCA TAGGCAGCGG CGGTGGAGGA GGCAGTGGGA GCGCGGTGGT GCCTGCCTCC 
CTGCAGCCTT ACGACGTGGA GATCCAGCGA GGAGAGAACG AGGGATTTGG TTTTGTCATC 
GTGTCGTCCG TTTCCAGGCC TGATGCTGGC ACTACTTTCG CTGGAAATGC TTGTGTGGCA 
ATGCCCCACA AAATAGGACG CATCATCGAG GGCAGCCCGG CGGACCGCTG CGGGAAGCTG 
AAGGTCGGGG ACAGAATACT GGCTGTTAAC GGCTGCTCCA TCACCAACAA GTCTCACTCA 
GACATCGTCA ACCTGATTAA GGAAGCCGGG AACACCGTCT CACTCAGGAT CATCCCAGGT 
GATGAATCTT CCAATGCTTC TCTGCTGACT AATGCTGAGA AGATAGCTAC CATCACCACG 
ACACACACAC CTCATTCCAC GGAGACCAGA AATAATTCAA AGTCAAAAGG AGCTCCTCCT 
CCACCCCCTT CACAGACCCA AACACAGGAA GCAGAGTTTT ATTCTGTGGA CCTCGAGAGG 
GACAGTAAAG GCTTCGGCTT CAGCTTAAGA GGAGGACGGG AGTACAACAT GGACCTGTAC 
GTGTTGAGAC TAGCAGAGGA TGGGGCTGCT GTCAGAAATG GCAAGATGAG GGTGGGAGAC 
GAGATCCTGG AGATTAATGG TGAGAGCACA AAGAGCATGA AGCATTCACG AGCCATTGAA 
CTGATCAAGA ACGGCGGTCG GAGGGCACGA CTTGTCCTAA AAAGGGGCGA TGGATCTGTA 
CCTGAATATG CGATGATGGC TCCTCATCTC GCTGTTGGTA CAATGAGGAA TGACAAGATG 
GGAGAGCCCT GTTTCTACCT AATGGGCCAA AATCAGACAA CGACGGCAGC AGTGACGGGC 
ACCCTCCCAC ACCCCGGGCA CAAAACACTC CGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108232031 ( XM_025004509.1 ) cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus) -> XP_024860277.1 Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X10, mRNA.

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CloneID	OKr34862
Clone ID Related Accession (Same CDS sequence)	XM_025004509.1
Accession Version	XM_025004509.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-03
Organism	Kryptolebias marmoratus(mangrove rivulus)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016094253.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Kryptolebias marmoratus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACGA AAGTGAAGGA GTGCGCCGTG 
GTTCGGGACC CCAGCGGGGA GCTGAACTTG GAGCTGCGCG GCGGGGCGCA GGACGGAGAG 
TTCGCCTACA TCGGACACAT CAGGGAGGAT GCTGTGCTCC ACCAGGACGG GAAACTGTCT 
GAGGGAGAGC TGCTGCTGGA GGTGGAGGGG CTGTCCGTGT CTGGATTACC TCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAAGGT CCCGTCAGGT TCAAAACTGT CCGCCAAGGT 
CACAAGCTCA ACAAGGACCT AAAGCACTTC CTGAGCTTGA GGTTTCAGAA GTCATCACCT 
GACCACGAGT TGCAGAAGAC TATCCGAGCC AATCTATATC GTCATGCCGT GCCTTGTACA 
ACCCGCGCCC CTCGAGACGG AGAGGTGCCA GGGGTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTTGAGGA GAGTGGCACA CTACTGGAAA TAGGAACGTA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC GCCGAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
ACGGGCTCCC ATCAGTCCAC CCCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAACGTC 
CGCGTAGTGC CTGCTGACGA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGAACCT TAGTGCGTAG CCTTTTCCCC TCTCCTTTGT TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGTAC CGGCATCCTT CGGCCATATC CTGTGCGTGA TAATGCTCTG 
GCCTGTGGTC TGAACAATGC CACCACCTCC ACTGTTGAAA GTGGGCAGCA GACGCTCGCA 
GAACCACAGG TCTCTCCAGA TGACCCTCTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACTGAGA ACGGGGAGCT GTACTTCATA GACACTGCAA AGCCTCACCA CCAGGACATG 
GCTGTGCATT TTCAGAGTGT CCACAACACA AAGACCACAT CATGGCTGGA TCCGCGGTGC 
CGAGACAAAG CCTCCAGGCC TTTGGAAGAG TGTGATGATG ATGAAGGGAT ACATACGGAA 
GACCTGGAAA GTGATCTAGA GCTGCCCCCA GGATGGGAAA GAATAGATGA CCCCGTGTAC 
GGAGTGTACT ATGTAGATCA TATAAACAGG AAGACCCAAT ATGAGAACCC AGTGGTGGAG 
GCGCGGCGCC AAAAACTCTT GGAGCAGCAG CAACAGCCGC AGCCTCCAGA AGGTGAGCGG 
TATATTCGAG AGTGGATAGA GGAGCACTCA GCTGCAGCAG CTCCTTTGGC AAACTATGCT 
CCAAACCACC TGGAGACCTA CAGGGACCCA CAAGTCCCTC CAGCTCTACC TCCAGGCCCC 
GCTGGCATCA AAAGAGGGAA ACCTTTCTTT ACGAGAAACC CAGCAGAGCT GAAGGGAACC 
TTCATCAACA CCAAGCTGAA GAAAAGTCAC CGTGGATTCG GGTTCACCGT GGTGGGAGGT 
GACGAGCCCG ACGAGTTCCT GCAGATCAAG AGTCTGGTGC TGGATGGGCC TGCGGCTGTT 
GATGGCAAGA TGGAGACAGG TGATGTGATT GTCAGCGTGA ATGACACTAT TGTGCTGGGC 
TACACTCATG CTCAGGTGGT GAAGATCTTC CAGTCCATCC CTATCGGCTC CATGGTGGAC 
CTGGCCTTAT GCCGTGGCTA CCCACTGCCC TTTGACCCAG ACGACCCAAA CACCAGCCTG 
GTGACCTCTG TGGCCATCTT GGACAAGGAA CCCATTATTG TCAATGGGCA AGAGACGTTT 
GACACGCCGC CTAACCAGGC TGGACCCCTT AACAGCATGC GTGAGCCACG GCCTCTCAGC 
CCCTCAGCTG AGGTGGCGTC CAACGGCTCC CATGGTTACT CCAGCGACGT GGTCACGTTG 
GCCTCGTCCA TAGCCACTCA GCCCGAGCTC ATCACCATCC ACATGGAGAA GGGGGACAAA 
GGTTTTGGCT TCACCATCGC TGACAGCCTA ACAGGCGGAG GCCAGAGGGT AAAGCAGATA 
GTGGACTATC CACGGTGCCG CGGCCTGAAA GAGGGGGACA TTCTAATGGA GGTCAACAAG 
AGAAATGTCC AGAATATGAG TCACAATCAG GTGGTGGATC TGCTGAGCAA ATGTCCAAGA 
GGCAGTGAGG TCACCATGTT GGTGCAAAGA GGAGTGGTGC CAGCCAAGAG GAGCCCAAAG 
CTGGTGCACC AACTGGAGAG AAAAGACAGT CAGAACAGCT CCCAGCACAG TGTGTGCAGC 
CATCGCAGCA CCCACACCGA TTCTCCCAGC CACCCACCCT CTGCCATGCC CAGTGAGGCA 
GTGGTCCCCA CTCCCGCTGC CCCCACTCAG CCTCTGCCAG TCTTGCCCCC CCAGGACCCC 
GCAGATGGCA TGCTCACCCT CCAGAAGAAG CCAGACCCTT TTAAGATCTG GGCACAGTCC 
AGGAGCATGT ATGAGAGTCG ACTACCAGAT TGCCAGGAGC AAGACATTTT CCTTTGGCGG 
AAAGATACAG GTTTTGGGTT TCGCATCTTG GGAGGAAATG AGCCTGGGGA GCCTATATAC 
ATAGGGCACA TAGTGAAGTA CGGGGCCGCA GATGAAGATG GCCGCCTGCG ATCGGGTGAC 
GAGCTCATCT GTGTGGACGG CACGGCTGTA GTGGGCAAAT CTCACCAGCT GGTGGTGCAG 
CTGATGCAGC AGGCAGCCAA GCAGGGCCAC GTCAACCTCA CTGTCAGACG CAAGACCCCC 
GGATACGGAG GGTCAAAAGG AGATGGTGAC GTTCCTCCAT CCCCAGCCTC CTCCCACCAC 
AGCAGCACCC AGGCACCCAG TCTGACGGAG GGAAAGCGCA CCCCTCAGGG CAGCCAGAAC 
TCACTCAACA CTGTCAGCTC CGGCAGCGGA TCCACCAGCG GCATAGGCAG CGGCGGTGGA 
GGAGGCAGTG GGAGCGCGGT GGTGCCTGCC TCCCTGCAGC CTTACGACGT GGAGATCCAG 
CGAGGAGAGA ACGAGGGATT TGGTTTTGTC ATCGTGTCGT CCGTTTCCAG GCCTGATGCT 
GGCACTACTT TCGCTGGAAA TGCTTGTGTG GCAATGCCCC ACAAAATAGG ACGCATCATC 
GAGGGCAGCC CGGCGGACCG CTGCGGGAAG CTGAAGGTCG GGGACAGAAT ACTGGCTGTT 
AACGGCTGCT CCATCACCAA CAAGTCTCAC TCAGACATCG TCAACCTGAT TAAGGAAGCC 
GGGAACACCG TCTCACTCAG GATCATCCCA GGTGATGAAT CTTCCAATGC TTCTCTGCTG 
ACTAATGCTG AGAAGATAGC TACCATCACC ACGACACACA CACCTCATTC CACGGAGACC 
AGAAATAATT CAAAGTCAAA AGGAGCTCCT CCTCCACCCC CTTCACAGAC CCAAACACAG 
GAAGCAGAGT TTTATTCTGT GGACCTCGAG AGGGACAGTA AAGGCTTCGG CTTCAGCTTA 
AGAGGAGGAC GGGAGTACAA CATGGACCTG TACGTGTTGA GACTAGCAGA GGATGGGGCT 
GCTGTCAGAA ATGGCAAGAT GAGGGTGGGA GACGAGATCC TGGAGATTAA TGGTGAGAGC 
ACAAAGAGCA TGAAGCATTC ACGAGCCATT GAACTGATCA AGAACGGCGG TCGGAGGGCA 
CGACTTGTCC TAAAAAGGGG CGATGGATCT GTACCTGAAT ATGCGATGAT GGCTCCTCAT 
CTCGCTGTTG GTACAATGAG GAATGACAAG ATGGGAGAGC CCTGTTTCTA CCTAATGGGC 
CAAAATCAGA CAACGACGGC AGCAGTGACG GGCACCCTCC CACACCCCGG GCACAAAACA 
CTCCGGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024860277.1
CDS	917..4828
Translation	MSKVIQKKNHWITKVKECAVVRDPSGELNLELRGGAQDGEFAYIGHIREDAVLHQDGKLSEGELLLEVEGLSVSGLPLYDIYTVINCCKGPVRFKTVRQGHKLNKDLKHFLSLRFQKSSPDHELQKTIRANLYRHAVPCTTRAPRDGEVPGVDYNFLSVEDFLQLEESGTLLEIGTYEGNYYGTPKPPRQPIIGTVTLSDTGSHQSTPKRTKSYNDMQNVRVVPADDDDDDQATEMNNSFTGTLVRSLFPSPLLCTGNPNDQDESRTGILRPYPVRDNALACGLNNATTSTVESGQQTLAEPQVSPDDPLGPLPENWEMAYTENGELYFIDTAKPHHQDMAVHFQSVHNTKTTSWLDPRCRDKASRPLEECDDDEGIHTEDLESDLELPPGWERIDDPVYGVYYVDHINRKTQYENPVVEARRQKLLEQQQQPQPPEGERYIREWIEEHSAAAAPLANYAPNHLETYRDPQVPPALPPGPAGIKRGKPFFTRNPAELKGTFINTKLKKSHRGFGFTVVGGDEPDEFLQIKSLVLDGPAAVDGKMETGDVIVSVNDTIVLGYTHAQVVKIFQSIPIGSMVDLALCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETFDTPPNQAGPLNSMREPRPLSPSAEVASNGSHGYSSDVVTLASSIATQPELITIHMEKGDKGFGFTIADSLTGGGQRVKQIVDYPRCRGLKEGDILMEVNKRNVQNMSHNQVVDLLSKCPRGSEVTMLVQRGVVPAKRSPKLVHQLERKDSQNSSQHSVCSHRSTHTDSPSHPPSAMPSEAVVPTPAAPTQPLPVLPPQDPADGMLTLQKKPDPFKIWAQSRSMYESRLPDCQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTPGYGGSKGDGDVPPSPASSHHSSTQAPSLTEGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGSAVVPASLQPYDVEIQRGENEGFGFVIVSSVSRPDAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVSLRIIPGDESSNASLLTNAEKIATITTTHTPHSTETRNNSKSKGAPPPPPSQTQTQEAEFYSVDLERDSKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKSMKHSRAIELIKNGGRRARLVLKRGDGSVPEYAMMAPHLAVGTMRNDKMGEPCFYLMGQNQTTTAAVTGTLPHPGHKTLRK

Target ORF information:

RefSeq Version	XM_025004509.1
Organism	Kryptolebias marmoratus(mangrove rivulus)
Definition	Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025004509.1

ORF Insert Sequence:

ATGTCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACGA AAGTGAAGGA GTGCGCCGTG 
GTTCGGGACC CCAGCGGGGA GCTGAACTTG GAGCTGCGCG GCGGGGCGCA GGACGGAGAG 
TTCGCCTACA TCGGACACAT CAGGGAGGAT GCTGTGCTCC ACCAGGACGG GAAACTGTCT 
GAGGGAGAGC TGCTGCTGGA GGTGGAGGGG CTGTCCGTGT CTGGATTACC TCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAAGGT CCCGTCAGGT TCAAAACTGT CCGCCAAGGT 
CACAAGCTCA ACAAGGACCT AAAGCACTTC CTGAGCTTGA GGTTTCAGAA GTCATCACCT 
GACCACGAGT TGCAGAAGAC TATCCGAGCC AATCTATATC GTCATGCCGT GCCTTGTACA 
ACCCGCGCCC CTCGAGACGG AGAGGTGCCA GGGGTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTTGAGGA GAGTGGCACA CTACTGGAAA TAGGAACGTA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC GCCGAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
ACGGGCTCCC ATCAGTCCAC CCCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAACGTC 
CGCGTAGTGC CTGCTGACGA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGAACCT TAGTGCGTAG CCTTTTCCCC TCTCCTTTGT TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGTAC CGGCATCCTT CGGCCATATC CTGTGCGTGA TAATGCTCTG 
GCCTGTGGTC TGAACAATGC CACCACCTCC ACTGTTGAAA GTGGGCAGCA GACGCTCGCA 
GAACCACAGG TCTCTCCAGA TGACCCTCTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACTGAGA ACGGGGAGCT GTACTTCATA GACACTGCAA AGCCTCACCA CCAGGACATG 
GCTGTGCATT TTCAGAGTGT CCACAACACA AAGACCACAT CATGGCTGGA TCCGCGGTGC 
CGAGACAAAG CCTCCAGGCC TTTGGAAGAG TGTGATGATG ATGAAGGGAT ACATACGGAA 
GACCTGGAAA GTGATCTAGA GCTGCCCCCA GGATGGGAAA GAATAGATGA CCCCGTGTAC 
GGAGTGTACT ATGTAGATCA TATAAACAGG AAGACCCAAT ATGAGAACCC AGTGGTGGAG 
GCGCGGCGCC AAAAACTCTT GGAGCAGCAG CAACAGCCGC AGCCTCCAGA AGGTGAGCGG 
TATATTCGAG AGTGGATAGA GGAGCACTCA GCTGCAGCAG CTCCTTTGGC AAACTATGCT 
CCAAACCACC TGGAGACCTA CAGGGACCCA CAAGTCCCTC CAGCTCTACC TCCAGGCCCC 
GCTGGCATCA AAAGAGGGAA ACCTTTCTTT ACGAGAAACC CAGCAGAGCT GAAGGGAACC 
TTCATCAACA CCAAGCTGAA GAAAAGTCAC CGTGGATTCG GGTTCACCGT GGTGGGAGGT 
GACGAGCCCG ACGAGTTCCT GCAGATCAAG AGTCTGGTGC TGGATGGGCC TGCGGCTGTT 
GATGGCAAGA TGGAGACAGG TGATGTGATT GTCAGCGTGA ATGACACTAT TGTGCTGGGC 
TACACTCATG CTCAGGTGGT GAAGATCTTC CAGTCCATCC CTATCGGCTC CATGGTGGAC 
CTGGCCTTAT GCCGTGGCTA CCCACTGCCC TTTGACCCAG ACGACCCAAA CACCAGCCTG 
GTGACCTCTG TGGCCATCTT GGACAAGGAA CCCATTATTG TCAATGGGCA AGAGACGTTT 
GACACGCCGC CTAACCAGGC TGGACCCCTT AACAGCATGC GTGAGCCACG GCCTCTCAGC 
CCCTCAGCTG AGGTGGCGTC CAACGGCTCC CATGGTTACT CCAGCGACGT GGTCACGTTG 
GCCTCGTCCA TAGCCACTCA GCCCGAGCTC ATCACCATCC ACATGGAGAA GGGGGACAAA 
GGTTTTGGCT TCACCATCGC TGACAGCCTA ACAGGCGGAG GCCAGAGGGT AAAGCAGATA 
GTGGACTATC CACGGTGCCG CGGCCTGAAA GAGGGGGACA TTCTAATGGA GGTCAACAAG 
AGAAATGTCC AGAATATGAG TCACAATCAG GTGGTGGATC TGCTGAGCAA ATGTCCAAGA 
GGCAGTGAGG TCACCATGTT GGTGCAAAGA GGAGTGGTGC CAGCCAAGAG GAGCCCAAAG 
CTGGTGCACC AACTGGAGAG AAAAGACAGT CAGAACAGCT CCCAGCACAG TGTGTGCAGC 
CATCGCAGCA CCCACACCGA TTCTCCCAGC CACCCACCCT CTGCCATGCC CAGTGAGGCA 
GTGGTCCCCA CTCCCGCTGC CCCCACTCAG CCTCTGCCAG TCTTGCCCCC CCAGGACCCC 
GCAGATGGCA TGCTCACCCT CCAGAAGAAG CCAGACCCTT TTAAGATCTG GGCACAGTCC 
AGGAGCATGT ATGAGAGTCG ACTACCAGAT TGCCAGGAGC AAGACATTTT CCTTTGGCGG 
AAAGATACAG GTTTTGGGTT TCGCATCTTG GGAGGAAATG AGCCTGGGGA GCCTATATAC 
ATAGGGCACA TAGTGAAGTA CGGGGCCGCA GATGAAGATG GCCGCCTGCG ATCGGGTGAC 
GAGCTCATCT GTGTGGACGG CACGGCTGTA GTGGGCAAAT CTCACCAGCT GGTGGTGCAG 
CTGATGCAGC AGGCAGCCAA GCAGGGCCAC GTCAACCTCA CTGTCAGACG CAAGACCCCC 
GGATACGGAG GGTCAAAAGG AGATGGTGAC GTTCCTCCAT CCCCAGCCTC CTCCCACCAC 
AGCAGCACCC AGGCACCCAG TCTGACGGAG GGAAAGCGCA CCCCTCAGGG CAGCCAGAAC 
TCACTCAACA CTGTCAGCTC CGGCAGCGGA TCCACCAGCG GCATAGGCAG CGGCGGTGGA 
GGAGGCAGTG GGAGCGCGGT GGTGCCTGCC TCCCTGCAGC CTTACGACGT GGAGATCCAG 
CGAGGAGAGA ACGAGGGATT TGGTTTTGTC ATCGTGTCGT CCGTTTCCAG GCCTGATGCT 
GGCACTACTT TCGCTGGAAA TGCTTGTGTG GCAATGCCCC ACAAAATAGG ACGCATCATC 
GAGGGCAGCC CGGCGGACCG CTGCGGGAAG CTGAAGGTCG GGGACAGAAT ACTGGCTGTT 
AACGGCTGCT CCATCACCAA CAAGTCTCAC TCAGACATCG TCAACCTGAT TAAGGAAGCC 
GGGAACACCG TCTCACTCAG GATCATCCCA GGTGATGAAT CTTCCAATGC TTCTCTGCTG 
ACTAATGCTG AGAAGATAGC TACCATCACC ACGACACACA CACCTCATTC CACGGAGACC 
AGAAATAATT CAAAGTCAAA AGGAGCTCCT CCTCCACCCC CTTCACAGAC CCAAACACAG 
GAAGCAGAGT TTTATTCTGT GGACCTCGAG AGGGACAGTA AAGGCTTCGG CTTCAGCTTA 
AGAGGAGGAC GGGAGTACAA CATGGACCTG TACGTGTTGA GACTAGCAGA GGATGGGGCT 
GCTGTCAGAA ATGGCAAGAT GAGGGTGGGA GACGAGATCC TGGAGATTAA TGGTGAGAGC 
ACAAAGAGCA TGAAGCATTC ACGAGCCATT GAACTGATCA AGAACGGCGG TCGGAGGGCA 
CGACTTGTCC TAAAAAGGGG CGATGGATCT GTACCTGAAT ATGCGATGAT GGCTCCTCAT 
CTCGCTGTTG GTACAATGAG GAATGACAAG ATGGGAGAGC CCTGTTTCTA CCTAATGGGC 
CAAAATCAGA CAACGACGGC AGCAGTGACG GGCACCCTCC CACACCCCGG GCACAAAACA 
CTCCGGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108232031 ( XM_017409517.2 ) cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus) -> XP_017265006.1 Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X1, mRNA.

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CloneID	OKr06768
Clone ID Related Accession (Same CDS sequence)	XM_017409517.2 , XM_017409517.1
Accession Version	XM_017409517.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-03
Organism	Kryptolebias marmoratus(mangrove rivulus)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016094253.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 4, 2018 this sequence version replaced XM_017409517.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Kryptolebias marmoratus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACGA AAGTGAAGGA GTGCGCCGTG 
GTTCGGGACC CCAGCGGGGA GCTGAACTTG GAGCTGCGCG GCGGGGCGCA GGACGGAGAG 
TTCGCCTACA TCGGACACAT CAGGGAGGAT GCTGTGCTCC ACCAGGACGG GAAACTGTCT 
GAGGGAGAGC TGCTGCTGGA GGTGGAGGGG CTGTCCGTGT CTGGATTACC TCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAAGGT CCCGTCAGGT TCAAAACTGT CCGCCAAGGT 
CACAAGCTCA ACAAGGACCT AAAGCACTTC CTGAGCTTGA GGTTTCAGAA GTCATCACCT 
GACCACGAGT TGCAGAAGAC TATCCGAGCC AATCTATATC GTCATGCCGT GCCTTGTACA 
ACCCGCGCCC CTCGAGACGG AGAGGTGCCA GGGGTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTTGAGGA GAGTGGCACA CTACTGGAAA TAGGAACGTA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC GCCGAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
ACGGGCTCCC ATCAGTCCAC CCCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAACGTC 
CGCGTAGTGC CTGCTGACGA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGAACCT TAGTGCGTAG CCTTTTCCCC TCTCCTTTGT TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGTAC CGGCATCCTT CGGCCATATC CTGTGCGTGA TAATGCTCTG 
GCCTGTGGTC TGAACAATGC CACCACCTCC ACTGTTGAAA GTGGGCAGCA GACGCTCGCA 
GAACCACAGG TCTCTCCAGA TGACCCTCTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACTGAGA ACGGGGAGCT GTACTTCATA GACACTGCAA AGCCTCACCA CCAGGACATG 
GCTGTGCATT TTCAGAGTGT CCACAACACA AAGACCACAT CATGGCTGGA TCCGCGGTGC 
CGAGACAAAG CCTCCAGGCC TTTGGAAGAG TGTGATGATG ATGAAGAAGG GATACATACG 
GAAGACCTGG AAAGTGATCT AGAGCTGCCC CCAGGATGGG AAAGAATAGA TGACCCCGTG 
TACGGAGTGT ACTATGTAGA TCATATAAAC AGGAAGACCC AATATGAGAA CCCAGTGGTG 
GAGGCGCGGC GCCAAAAACT CTTGGAGCAG CAGCAACAGC CGCAGCCTCC AGAAGGTGAG 
CGGTATATTC GAGAGTGGAT AGAGGAGCAC TCAGCTGCAG CAGCTCCTTT GGCAAACTAT 
GCTCCAAACC ACCTGGAGAC CTACAGGGAC CCACAAGTCC CTCCAGCTCT ACCTCCAGGC 
CCCGCTGGCA TCAAAAGAGG GAAACCTTTC TTTACGAGAA ACCCAGCAGA GCTGAAGGGA 
ACCTTCATCA ACACCAAGCT GAAGAAAAGT CACCGTGGAT TCGGGTTCAC CGTGGTGGGA 
GGTGACGAGC CCGACGAGTT CCTGCAGATC AAGAGTCTGG TGCTGGATGG GCCTGCGGCT 
GTTGATGGCA AGATGGAGAC AGGTGATGTG ATTGTCAGCG TGAATGACAC TATTGTGCTG 
GGCTACACTC ATGCTCAGGT GGTGAAGATC TTCCAGTCCA TCCCTATCGG CTCCATGGTG 
GACCTGGCCT TATGCCGTGG CTACCCACTG CCCTTTGACC CAGACGACCC AAACACCAGC 
CTGGTGACCT CTGTGGCCAT CTTGGACAAG GAACCCATTA TTGTCAATGG GCAAGAGACG 
TTTGACACGC CGCCTAACCA GGCTGGACCC CTTAACAGCA TGCGTGAGCC ACGGCCTCTC 
AGCCCCTCAG CTGAGGTGGC GTCCAACGGC TCCCATGGTT ACTCCAGCGA CGTGGTCACG 
TTGGCCTCGT CCATAGCCAC TCAGCCCGAG CTCATCACCA TCCACATGGA GAAGGGGGAC 
AAAGGTTTTG GCTTCACCAT CGCTGACAGC CTAACAGGCG GAGGCCAGAG GGTAAAGCAG 
ATAGTGGACT ATCCACGGTG CCGCGGCCTG AAAGAGGGGG ACATTCTAAT GGAGGTCAAC 
AAGAGAAATG TCCAGAATAT GAGTCACAAT CAGGTGGTGG ATCTGCTGAG CAAATGTCCA 
AGAGGCAGTG AGGTCACCAT GTTGGTGCAA AGAGGAGTGG TGCCAGCCAA GAGGAGCCCA 
AAGCTGGTGC ACCAACTGGA GAGAAAAGAC AGTCAGAACA GCTCCCAGCA CAGTGTGTGC 
AGCCATCGCA GCACCCACAC CGATTCTCCC AGCCACCCAC CCTCTGCCAT GCCCAGTGAG 
GCAGTGGTCC CCACTCCCGC TGCCCCCACT CAGCCTCTGC CAGTCTTGCC CCCCCAGGAC 
CCCGCAGATG GCATGCTCAC CCTCCAGAAG AAGCCAGACC CTTTTAAGAT CTGGGCACAG 
TCCAGGAGCA TGTATGAGAG TCGACTACCA GATTGCCAGG AGCAAGACAT TTTCCTTTGG 
CGGAAAGATA CAGGTTTTGG GTTTCGCATC TTGGGAGGAA ATGAGCCTGG GGAGCCTATA 
TACATAGGGC ACATAGTGAA GTACGGGGCC GCAGATGAAG ATGGCCGCCT GCGATCGGGT 
GACGAGCTCA TCTGTGTGGA CGGCACGGCT GTAGTGGGCA AATCTCACCA GCTGGTGGTG 
CAGCTGATGC AGCAGGCAGC CAAGCAGGGC CACGTCAACC TCACTGTCAG ACGCAAGACC 
CCCGGATACG GAGGGTCAAA AGGAGATGGT GACGTTCCTC CATCCCCAGC CTCCTCCCAC 
CACAGCAGCA CCCAGGCACC CAGTCTGACG GAGGGAAAGC GCACCCCTCA GGGCAGCCAG 
AACTCACTCA ACACTGTCAG CTCCGGCAGC GGATCCACCA GCGGCATAGG CAGCGGCGGT 
GGAGGAGGCA GTGGGAGCGC GGTGGTGCCT GCCTCCCTGC AGCCTTACGA CGTGGAGATC 
CAGCGAGGAG AGAACGAGGG ATTTGGTTTT GTCATCGTGT CGTCCGTTTC CAGGCCTGAT 
GCTGGCACTA CTTTCGCTGG AAATGCTTGT GTGGCAATGC CCCACAAAAT AGGACGCATC 
ATCGAGGGCA GCCCGGCGGA CCGCTGCGGG AAGCTGAAGG TCGGGGACAG AATACTGGCT 
GTTAACGGCT GCTCCATCAC CAACAAGTCT CACTCAGACA TCGTCAACCT GATTAAGGAA 
GCCGGGAACA CCGTCTCACT CAGGATCATC CCAGGTGATG AATCTTCCAA TGCTTCTCTG 
CTGACTAATG CTGAGAAGAT AGCTACCATC ACCACGACAC ACACACCTCA TTCCACGGAG 
ACCAGAAATA ATTCAAAGTC AAAAGGAGCT CCTCCTCCAC CCCCTTCACA GACCCAAACA 
CAGGAAGCAG AGTTTTATTC TGTGGACCTC GAGAGGGACA GTAAAGGCTT CGGCTTCAGC 
TTAAGAGGAG GACGGGAGTA CAACATGGAC CTGTACGTGT TGAGACTAGC AGAGGATGGG 
GCTGCTGTCA GAAATGGCAA GATGAGGGTG GGAGACGAGA TCCTGGAGAT TAATGGTGAG 
AGCACAAAGA GCATGAAGCA TTCACGAGCC ATTGAACTGA TCAAGAACGG CGGTCGGAGG 
GCACGACTTG TCCTAAAAAG GGGCGATGGA TCTGTACCTG AATATGACGG CAGCAGTGAC 
GGGCACCCTC CCACACCCCG GGCACAAAAC ACTCCGGAAG TGAGAACGTT GCCACCCAGC 
AGCCGATATC ACACCTCATT GGAGTCCAGT TATCCACCAG ACCTTCACAA ATCATCACGC 
CTGGAGGAGG CTGCTTGTGG CCGAGGTATG GACAAGAACA GGGACAGGGC TGGTTCAGAT 
CAGATGGAAT TCCAAGGCCG ACAATTTAAC CCCCATCACC ATTACACTTG GAATAATAAT 
CACAGTCAAA GTACCCCCAG ACAGCAGTCC CCTGACAGTA ATAGCACTAG CAGTGGCAAC 
AAGAAGACCA GAGGCCGTAA AGTCAAGAAG TCTGAATCGG AGAGTGGCAT CTCTAAACTT 
CTAAAGACTC TGAGGAAGGA CAGCTCGGGA AAAAAGGCTG CAGCCGCCGA AAAACTAAGG 
GGCCGAGAGC TTTCAAGCAG CGGTTCTACT TTGGATGAGA AGTATCTTCG GCAGCGTCGT 
GGGTCCCTCG ACCGCTCACC AACCAGAAAG CGCACCTCGT CTCCAGATCG TCGACGGGCG 
AAATCCATGG ACCGCAGGGC AGAGCGAGGA CATCGTGACC GCACACCTGA GAGGGAACAC 
GATGATGGAG GGTTTCTCTC AGTCACCCCT GAACGGAGGT TCCACGAGCG AAGGCTCCTC 
AAGGACTCAC AGATAGCTGG ACGAGTCAGT GAGGCTTCAA CCCCCGAGCG CGCCAACAAC 
AGCATGGAAT CCTTGTCTCG TTCCAGGGGC CGCACGGACG ATAAAGCCAC AAAGAACGGC 
AACCTTCTGA GAGACTCTTC TTCAGAGAGG AGAGAGGACC GACATCGCAT GAATGGGACA 
ATGGAGAGGC GATACAGAGC GGAGCGGGAC TCTTCCCCTG ACAGCAACTA CAGCCGGGAC 
GAGCTGAACT ATGCAGAAGC ACCTAGACTG AAGGACTACT ACAGCATGAT GAAGAACAAC 
GCTGCACACA ACAATGCTAC CTACAACAAC GCAGGCCATA ACAATAACAC AGTAGGCCGC 
AGCTCAAACC AGGTCCCTGA GCCCAAGAGG AGGCTGTACA AAGAAAGTCC TAAAGACCTC 
AGCATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017265006.1
CDS	893..5761
Translation	MSKVIQKKNHWITKVKECAVVRDPSGELNLELRGGAQDGEFAYIGHIREDAVLHQDGKLSEGELLLEVEGLSVSGLPLYDIYTVINCCKGPVRFKTVRQGHKLNKDLKHFLSLRFQKSSPDHELQKTIRANLYRHAVPCTTRAPRDGEVPGVDYNFLSVEDFLQLEESGTLLEIGTYEGNYYGTPKPPRQPIIGTVTLSDTGSHQSTPKRTKSYNDMQNVRVVPADDDDDDQATEMNNSFTGTLVRSLFPSPLLCTGNPNDQDESRTGILRPYPVRDNALACGLNNATTSTVESGQQTLAEPQVSPDDPLGPLPENWEMAYTENGELYFIDTAKPHHQDMAVHFQSVHNTKTTSWLDPRCRDKASRPLEECDDDEEGIHTEDLESDLELPPGWERIDDPVYGVYYVDHINRKTQYENPVVEARRQKLLEQQQQPQPPEGERYIREWIEEHSAAAAPLANYAPNHLETYRDPQVPPALPPGPAGIKRGKPFFTRNPAELKGTFINTKLKKSHRGFGFTVVGGDEPDEFLQIKSLVLDGPAAVDGKMETGDVIVSVNDTIVLGYTHAQVVKIFQSIPIGSMVDLALCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETFDTPPNQAGPLNSMREPRPLSPSAEVASNGSHGYSSDVVTLASSIATQPELITIHMEKGDKGFGFTIADSLTGGGQRVKQIVDYPRCRGLKEGDILMEVNKRNVQNMSHNQVVDLLSKCPRGSEVTMLVQRGVVPAKRSPKLVHQLERKDSQNSSQHSVCSHRSTHTDSPSHPPSAMPSEAVVPTPAAPTQPLPVLPPQDPADGMLTLQKKPDPFKIWAQSRSMYESRLPDCQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTPGYGGSKGDGDVPPSPASSHHSSTQAPSLTEGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGSAVVPASLQPYDVEIQRGENEGFGFVIVSSVSRPDAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVSLRIIPGDESSNASLLTNAEKIATITTTHTPHSTETRNNSKSKGAPPPPPSQTQTQEAEFYSVDLERDSKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKSMKHSRAIELIKNGGRRARLVLKRGDGSVPEYDGSSDGHPPTPRAQNTPEVRTLPPSSRYHTSLESSYPPDLHKSSRLEEAACGRGMDKNRDRAGSDQMEFQGRQFNPHHHYTWNNNHSQSTPRQQSPDSNSTSSGNKKTRGRKVKKSESESGISKLLKTLRKDSSGKKAAAAEKLRGRELSSSGSTLDEKYLRQRRGSLDRSPTRKRTSSPDRRRAKSMDRRAERGHRDRTPEREHDDGGFLSVTPERRFHERRLLKDSQIAGRVSEASTPERANNSMESLSRSRGRTDDKATKNGNLLRDSSSERREDRHRMNGTMERRYRAERDSSPDSNYSRDELNYAEAPRLKDYYSMMKNNAAHNNATYNNAGHNNNTVGRSSNQVPEPKRRLYKESPKDLSI

Target ORF information:

RefSeq Version	XM_017409517.2
Organism	Kryptolebias marmoratus(mangrove rivulus)
Definition	Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017409517.2

ORF Insert Sequence:

ATGTCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACGA AAGTGAAGGA GTGCGCCGTG 
GTTCGGGACC CCAGCGGGGA GCTGAACTTG GAGCTGCGCG GCGGGGCGCA GGACGGAGAG 
TTCGCCTACA TCGGACACAT CAGGGAGGAT GCTGTGCTCC ACCAGGACGG GAAACTGTCT 
GAGGGAGAGC TGCTGCTGGA GGTGGAGGGG CTGTCCGTGT CTGGATTACC TCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAAGGT CCCGTCAGGT TCAAAACTGT CCGCCAAGGT 
CACAAGCTCA ACAAGGACCT AAAGCACTTC CTGAGCTTGA GGTTTCAGAA GTCATCACCT 
GACCACGAGT TGCAGAAGAC TATCCGAGCC AATCTATATC GTCATGCCGT GCCTTGTACA 
ACCCGCGCCC CTCGAGACGG AGAGGTGCCA GGGGTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTTGAGGA GAGTGGCACA CTACTGGAAA TAGGAACGTA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC GCCGAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
ACGGGCTCCC ATCAGTCCAC CCCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAACGTC 
CGCGTAGTGC CTGCTGACGA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGAACCT TAGTGCGTAG CCTTTTCCCC TCTCCTTTGT TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGTAC CGGCATCCTT CGGCCATATC CTGTGCGTGA TAATGCTCTG 
GCCTGTGGTC TGAACAATGC CACCACCTCC ACTGTTGAAA GTGGGCAGCA GACGCTCGCA 
GAACCACAGG TCTCTCCAGA TGACCCTCTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACTGAGA ACGGGGAGCT GTACTTCATA GACACTGCAA AGCCTCACCA CCAGGACATG 
GCTGTGCATT TTCAGAGTGT CCACAACACA AAGACCACAT CATGGCTGGA TCCGCGGTGC 
CGAGACAAAG CCTCCAGGCC TTTGGAAGAG TGTGATGATG ATGAAGAAGG GATACATACG 
GAAGACCTGG AAAGTGATCT AGAGCTGCCC CCAGGATGGG AAAGAATAGA TGACCCCGTG 
TACGGAGTGT ACTATGTAGA TCATATAAAC AGGAAGACCC AATATGAGAA CCCAGTGGTG 
GAGGCGCGGC GCCAAAAACT CTTGGAGCAG CAGCAACAGC CGCAGCCTCC AGAAGGTGAG 
CGGTATATTC GAGAGTGGAT AGAGGAGCAC TCAGCTGCAG CAGCTCCTTT GGCAAACTAT 
GCTCCAAACC ACCTGGAGAC CTACAGGGAC CCACAAGTCC CTCCAGCTCT ACCTCCAGGC 
CCCGCTGGCA TCAAAAGAGG GAAACCTTTC TTTACGAGAA ACCCAGCAGA GCTGAAGGGA 
ACCTTCATCA ACACCAAGCT GAAGAAAAGT CACCGTGGAT TCGGGTTCAC CGTGGTGGGA 
GGTGACGAGC CCGACGAGTT CCTGCAGATC AAGAGTCTGG TGCTGGATGG GCCTGCGGCT 
GTTGATGGCA AGATGGAGAC AGGTGATGTG ATTGTCAGCG TGAATGACAC TATTGTGCTG 
GGCTACACTC ATGCTCAGGT GGTGAAGATC TTCCAGTCCA TCCCTATCGG CTCCATGGTG 
GACCTGGCCT TATGCCGTGG CTACCCACTG CCCTTTGACC CAGACGACCC AAACACCAGC 
CTGGTGACCT CTGTGGCCAT CTTGGACAAG GAACCCATTA TTGTCAATGG GCAAGAGACG 
TTTGACACGC CGCCTAACCA GGCTGGACCC CTTAACAGCA TGCGTGAGCC ACGGCCTCTC 
AGCCCCTCAG CTGAGGTGGC GTCCAACGGC TCCCATGGTT ACTCCAGCGA CGTGGTCACG 
TTGGCCTCGT CCATAGCCAC TCAGCCCGAG CTCATCACCA TCCACATGGA GAAGGGGGAC 
AAAGGTTTTG GCTTCACCAT CGCTGACAGC CTAACAGGCG GAGGCCAGAG GGTAAAGCAG 
ATAGTGGACT ATCCACGGTG CCGCGGCCTG AAAGAGGGGG ACATTCTAAT GGAGGTCAAC 
AAGAGAAATG TCCAGAATAT GAGTCACAAT CAGGTGGTGG ATCTGCTGAG CAAATGTCCA 
AGAGGCAGTG AGGTCACCAT GTTGGTGCAA AGAGGAGTGG TGCCAGCCAA GAGGAGCCCA 
AAGCTGGTGC ACCAACTGGA GAGAAAAGAC AGTCAGAACA GCTCCCAGCA CAGTGTGTGC 
AGCCATCGCA GCACCCACAC CGATTCTCCC AGCCACCCAC CCTCTGCCAT GCCCAGTGAG 
GCAGTGGTCC CCACTCCCGC TGCCCCCACT CAGCCTCTGC CAGTCTTGCC CCCCCAGGAC 
CCCGCAGATG GCATGCTCAC CCTCCAGAAG AAGCCAGACC CTTTTAAGAT CTGGGCACAG 
TCCAGGAGCA TGTATGAGAG TCGACTACCA GATTGCCAGG AGCAAGACAT TTTCCTTTGG 
CGGAAAGATA CAGGTTTTGG GTTTCGCATC TTGGGAGGAA ATGAGCCTGG GGAGCCTATA 
TACATAGGGC ACATAGTGAA GTACGGGGCC GCAGATGAAG ATGGCCGCCT GCGATCGGGT 
GACGAGCTCA TCTGTGTGGA CGGCACGGCT GTAGTGGGCA AATCTCACCA GCTGGTGGTG 
CAGCTGATGC AGCAGGCAGC CAAGCAGGGC CACGTCAACC TCACTGTCAG ACGCAAGACC 
CCCGGATACG GAGGGTCAAA AGGAGATGGT GACGTTCCTC CATCCCCAGC CTCCTCCCAC 
CACAGCAGCA CCCAGGCACC CAGTCTGACG GAGGGAAAGC GCACCCCTCA GGGCAGCCAG 
AACTCACTCA ACACTGTCAG CTCCGGCAGC GGATCCACCA GCGGCATAGG CAGCGGCGGT 
GGAGGAGGCA GTGGGAGCGC GGTGGTGCCT GCCTCCCTGC AGCCTTACGA CGTGGAGATC 
CAGCGAGGAG AGAACGAGGG ATTTGGTTTT GTCATCGTGT CGTCCGTTTC CAGGCCTGAT 
GCTGGCACTA CTTTCGCTGG AAATGCTTGT GTGGCAATGC CCCACAAAAT AGGACGCATC 
ATCGAGGGCA GCCCGGCGGA CCGCTGCGGG AAGCTGAAGG TCGGGGACAG AATACTGGCT 
GTTAACGGCT GCTCCATCAC CAACAAGTCT CACTCAGACA TCGTCAACCT GATTAAGGAA 
GCCGGGAACA CCGTCTCACT CAGGATCATC CCAGGTGATG AATCTTCCAA TGCTTCTCTG 
CTGACTAATG CTGAGAAGAT AGCTACCATC ACCACGACAC ACACACCTCA TTCCACGGAG 
ACCAGAAATA ATTCAAAGTC AAAAGGAGCT CCTCCTCCAC CCCCTTCACA GACCCAAACA 
CAGGAAGCAG AGTTTTATTC TGTGGACCTC GAGAGGGACA GTAAAGGCTT CGGCTTCAGC 
TTAAGAGGAG GACGGGAGTA CAACATGGAC CTGTACGTGT TGAGACTAGC AGAGGATGGG 
GCTGCTGTCA GAAATGGCAA GATGAGGGTG GGAGACGAGA TCCTGGAGAT TAATGGTGAG 
AGCACAAAGA GCATGAAGCA TTCACGAGCC ATTGAACTGA TCAAGAACGG CGGTCGGAGG 
GCACGACTTG TCCTAAAAAG GGGCGATGGA TCTGTACCTG AATATGACGG CAGCAGTGAC 
GGGCACCCTC CCACACCCCG GGCACAAAAC ACTCCGGAAG TGAGAACGTT GCCACCCAGC 
AGCCGATATC ACACCTCATT GGAGTCCAGT TATCCACCAG ACCTTCACAA ATCATCACGC 
CTGGAGGAGG CTGCTTGTGG CCGAGGTATG GACAAGAACA GGGACAGGGC TGGTTCAGAT 
CAGATGGAAT TCCAAGGCCG ACAATTTAAC CCCCATCACC ATTACACTTG GAATAATAAT 
CACAGTCAAA GTACCCCCAG ACAGCAGTCC CCTGACAGTA ATAGCACTAG CAGTGGCAAC 
AAGAAGACCA GAGGCCGTAA AGTCAAGAAG TCTGAATCGG AGAGTGGCAT CTCTAAACTT 
CTAAAGACTC TGAGGAAGGA CAGCTCGGGA AAAAAGGCTG CAGCCGCCGA AAAACTAAGG 
GGCCGAGAGC TTTCAAGCAG CGGTTCTACT TTGGATGAGA AGTATCTTCG GCAGCGTCGT 
GGGTCCCTCG ACCGCTCACC AACCAGAAAG CGCACCTCGT CTCCAGATCG TCGACGGGCG 
AAATCCATGG ACCGCAGGGC AGAGCGAGGA CATCGTGACC GCACACCTGA GAGGGAACAC 
GATGATGGAG GGTTTCTCTC AGTCACCCCT GAACGGAGGT TCCACGAGCG AAGGCTCCTC 
AAGGACTCAC AGATAGCTGG ACGAGTCAGT GAGGCTTCAA CCCCCGAGCG CGCCAACAAC 
AGCATGGAAT CCTTGTCTCG TTCCAGGGGC CGCACGGACG ATAAAGCCAC AAAGAACGGC 
AACCTTCTGA GAGACTCTTC TTCAGAGAGG AGAGAGGACC GACATCGCAT GAATGGGACA 
ATGGAGAGGC GATACAGAGC GGAGCGGGAC TCTTCCCCTG ACAGCAACTA CAGCCGGGAC 
GAGCTGAACT ATGCAGAAGC ACCTAGACTG AAGGACTACT ACAGCATGAT GAAGAACAAC 
GCTGCACACA ACAATGCTAC CTACAACAAC GCAGGCCATA ACAATAACAC AGTAGGCCGC 
AGCTCAAACC AGGTCCCTGA GCCCAAGAGG AGGCTGTACA AAGAAAGTCC TAAAGACCTC 
AGCATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108232031 ( XM_017409517.1 ) cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus) -> XP_017265006.1 Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OKr06768
Clone ID Related Accession (Same CDS sequence)	XM_017409517.2 , XM_017409517.1
Accession Version	XM_017409517.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-27
Organism	Kryptolebias marmoratus(mangrove rivulus)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016094253.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Kryptolebias marmoratus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACGA AAGTGAAGGA GTGCGCCGTG 
GTTCGGGACC CCAGCGGGGA GCTGAACTTG GAGCTGCGCG GCGGGGCGCA GGACGGAGAG 
TTCGCCTACA TCGGACACAT CAGGGAGGAT GCTGTGCTCC ACCAGGACGG GAAACTGTCT 
GAGGGAGAGC TGCTGCTGGA GGTGGAGGGG CTGTCCGTGT CTGGATTACC TCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAAGGT CCCGTCAGGT TCAAAACTGT CCGCCAAGGT 
CACAAGCTCA ACAAGGACCT AAAGCACTTC CTGAGCTTGA GGTTTCAGAA GTCATCACCT 
GACCACGAGT TGCAGAAGAC TATCCGAGCC AATCTATATC GTCATGCCGT GCCTTGTACA 
ACCCGCGCCC CTCGAGACGG AGAGGTGCCA GGGGTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTTGAGGA GAGTGGCACA CTACTGGAAA TAGGAACGTA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC GCCGAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
ACGGGCTCCC ATCAGTCCAC CCCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAACGTC 
CGCGTAGTGC CTGCTGACGA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGAACCT TAGTGCGTAG CCTTTTCCCC TCTCCTTTGT TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGTAC CGGCATCCTT CGGCCATATC CTGTGCGTGA TAATGCTCTG 
GCCTGTGGTC TGAACAATGC CACCACCTCC ACTGTTGAAA GTGGGCAGCA GACGCTCGCA 
GAACCACAGG TCTCTCCAGA TGACCCTCTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACTGAGA ACGGGGAGCT GTACTTCATA GACACTGCAA AGCCTCACCA CCAGGACATG 
GCTGTGCATT TTCAGAGTGT CCACAACACA AAGACCACAT CATGGCTGGA TCCGCGGTGC 
CGAGACAAAG CCTCCAGGCC TTTGGAAGAG TGTGATGATG ATGAAGAAGG GATACATACG 
GAAGACCTGG AAAGTGATCT AGAGCTGCCC CCAGGATGGG AAAGAATAGA TGACCCCGTG 
TACGGAGTGT ACTATGTAGA TCATATAAAC AGGAAGACCC AATATGAGAA CCCAGTGGTG 
GAGGCGCGGC GCCAAAAACT CTTGGAGCAG CAGCAACAGC CGCAGCCTCC AGAAGGTGAG 
CGGTATATTC GAGAGTGGAT AGAGGAGCAC TCAGCTGCAG CAGCTCCTTT GGCAAACTAT 
GCTCCAAACC ACCTGGAGAC CTACAGGGAC CCACAAGTCC CTCCAGCTCT ACCTCCAGGC 
CCCGCTGGCA TCAAAAGAGG GAAACCTTTC TTTACGAGAA ACCCAGCAGA GCTGAAGGGA 
ACCTTCATCA ACACCAAGCT GAAGAAAAGT CACCGTGGAT TCGGGTTCAC CGTGGTGGGA 
GGTGACGAGC CCGACGAGTT CCTGCAGATC AAGAGTCTGG TGCTGGATGG GCCTGCGGCT 
GTTGATGGCA AGATGGAGAC AGGTGATGTG ATTGTCAGCG TGAATGACAC TATTGTGCTG 
GGCTACACTC ATGCTCAGGT GGTGAAGATC TTCCAGTCCA TCCCTATCGG CTCCATGGTG 
GACCTGGCCT TATGCCGTGG CTACCCACTG CCCTTTGACC CAGACGACCC AAACACCAGC 
CTGGTGACCT CTGTGGCCAT CTTGGACAAG GAACCCATTA TTGTCAATGG GCAAGAGACG 
TTTGACACGC CGCCTAACCA GGCTGGACCC CTTAACAGCA TGCGTGAGCC ACGGCCTCTC 
AGCCCCTCAG CTGAGGTGGC GTCCAACGGC TCCCATGGTT ACTCCAGCGA CGTGGTCACG 
TTGGCCTCGT CCATAGCCAC TCAGCCCGAG CTCATCACCA TCCACATGGA GAAGGGGGAC 
AAAGGTTTTG GCTTCACCAT CGCTGACAGC CTAACAGGCG GAGGCCAGAG GGTAAAGCAG 
ATAGTGGACT ATCCACGGTG CCGCGGCCTG AAAGAGGGGG ACATTCTAAT GGAGGTCAAC 
AAGAGAAATG TCCAGAATAT GAGTCACAAT CAGGTGGTGG ATCTGCTGAG CAAATGTCCA 
AGAGGCAGTG AGGTCACCAT GTTGGTGCAA AGAGGAGTGG TGCCAGCCAA GAGGAGCCCA 
AAGCTGGTGC ACCAACTGGA GAGAAAAGAC AGTCAGAACA GCTCCCAGCA CAGTGTGTGC 
AGCCATCGCA GCACCCACAC CGATTCTCCC AGCCACCCAC CCTCTGCCAT GCCCAGTGAG 
GCAGTGGTCC CCACTCCCGC TGCCCCCACT CAGCCTCTGC CAGTCTTGCC CCCCCAGGAC 
CCCGCAGATG GCATGCTCAC CCTCCAGAAG AAGCCAGACC CTTTTAAGAT CTGGGCACAG 
TCCAGGAGCA TGTATGAGAG TCGACTACCA GATTGCCAGG AGCAAGACAT TTTCCTTTGG 
CGGAAAGATA CAGGTTTTGG GTTTCGCATC TTGGGAGGAA ATGAGCCTGG GGAGCCTATA 
TACATAGGGC ACATAGTGAA GTACGGGGCC GCAGATGAAG ATGGCCGCCT GCGATCGGGT 
GACGAGCTCA TCTGTGTGGA CGGCACGGCT GTAGTGGGCA AATCTCACCA GCTGGTGGTG 
CAGCTGATGC AGCAGGCAGC CAAGCAGGGC CACGTCAACC TCACTGTCAG ACGCAAGACC 
CCCGGATACG GAGGGTCAAA AGGAGATGGT GACGTTCCTC CATCCCCAGC CTCCTCCCAC 
CACAGCAGCA CCCAGGCACC CAGTCTGACG GAGGGAAAGC GCACCCCTCA GGGCAGCCAG 
AACTCACTCA ACACTGTCAG CTCCGGCAGC GGATCCACCA GCGGCATAGG CAGCGGCGGT 
GGAGGAGGCA GTGGGAGCGC GGTGGTGCCT GCCTCCCTGC AGCCTTACGA CGTGGAGATC 
CAGCGAGGAG AGAACGAGGG ATTTGGTTTT GTCATCGTGT CGTCCGTTTC CAGGCCTGAT 
GCTGGCACTA CTTTCGCTGG AAATGCTTGT GTGGCAATGC CCCACAAAAT AGGACGCATC 
ATCGAGGGCA GCCCGGCGGA CCGCTGCGGG AAGCTGAAGG TCGGGGACAG AATACTGGCT 
GTTAACGGCT GCTCCATCAC CAACAAGTCT CACTCAGACA TCGTCAACCT GATTAAGGAA 
GCCGGGAACA CCGTCTCACT CAGGATCATC CCAGGTGATG AATCTTCCAA TGCTTCTCTG 
CTGACTAATG CTGAGAAGAT AGCTACCATC ACCACGACAC ACACACCTCA TTCCACGGAG 
ACCAGAAATA ATTCAAAGTC AAAAGGAGCT CCTCCTCCAC CCCCTTCACA GACCCAAACA 
CAGGAAGCAG AGTTTTATTC TGTGGACCTC GAGAGGGACA GTAAAGGCTT CGGCTTCAGC 
TTAAGAGGAG GACGGGAGTA CAACATGGAC CTGTACGTGT TGAGACTAGC AGAGGATGGG 
GCTGCTGTCA GAAATGGCAA GATGAGGGTG GGAGACGAGA TCCTGGAGAT TAATGGTGAG 
AGCACAAAGA GCATGAAGCA TTCACGAGCC ATTGAACTGA TCAAGAACGG CGGTCGGAGG 
GCACGACTTG TCCTAAAAAG GGGCGATGGA TCTGTACCTG AATATGACGG CAGCAGTGAC 
GGGCACCCTC CCACACCCCG GGCACAAAAC ACTCCGGAAG TGAGAACGTT GCCACCCAGC 
AGCCGATATC ACACCTCATT GGAGTCCAGT TATCCACCAG ACCTTCACAA ATCATCACGC 
CTGGAGGAGG CTGCTTGTGG CCGAGGTATG GACAAGAACA GGGACAGGGC TGGTTCAGAT 
CAGATGGAAT TCCAAGGCCG ACAATTTAAC CCCCATCACC ATTACACTTG GAATAATAAT 
CACAGTCAAA GTACCCCCAG ACAGCAGTCC CCTGACAGTA ATAGCACTAG CAGTGGCAAC 
AAGAAGACCA GAGGCCGTAA AGTCAAGAAG TCTGAATCGG AGAGTGGCAT CTCTAAACTT 
CTAAAGACTC TGAGGAAGGA CAGCTCGGGA AAAAAGGCTG CAGCCGCCGA AAAACTAAGG 
GGCCGAGAGC TTTCAAGCAG CGGTTCTACT TTGGATGAGA AGTATCTTCG GCAGCGTCGT 
GGGTCCCTCG ACCGCTCACC AACCAGAAAG CGCACCTCGT CTCCAGATCG TCGACGGGCG 
AAATCCATGG ACCGCAGGGC AGAGCGAGGA CATCGTGACC GCACACCTGA GAGGGAACAC 
GATGATGGAG GGTTTCTCTC AGTCACCCCT GAACGGAGGT TCCACGAGCG AAGGCTCCTC 
AAGGACTCAC AGATAGCTGG ACGAGTCAGT GAGGCTTCAA CCCCCGAGCG CGCCAACAAC 
AGCATGGAAT CCTTGTCTCG TTCCAGGGGC CGCACGGACG ATAAAGCCAC AAAGAACGGC 
AACCTTCTGA GAGACTCTTC TTCAGAGAGG AGAGAGGACC GACATCGCAT GAATGGGACA 
ATGGAGAGGC GATACAGAGC GGAGCGGGAC TCTTCCCCTG ACAGCAACTA CAGCCGGGAC 
GAGCTGAACT ATGCAGAAGC ACCTAGACTG AAGGACTACT ACAGCATGAT GAAGAACAAC 
GCTGCACACA ACAATGCTAC CTACAACAAC GCAGGCCATA ACAATAACAC AGTAGGCCGC 
AGCTCAAACC AGGTCCCTGA GCCCAAGAGG AGGCTGTACA AAGAAAGTCC TAAAGACCTC 
AGCATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017265006.1
CDS	775..5643
Translation	MSKVIQKKNHWITKVKECAVVRDPSGELNLELRGGAQDGEFAYIGHIREDAVLHQDGKLSEGELLLEVEGLSVSGLPLYDIYTVINCCKGPVRFKTVRQGHKLNKDLKHFLSLRFQKSSPDHELQKTIRANLYRHAVPCTTRAPRDGEVPGVDYNFLSVEDFLQLEESGTLLEIGTYEGNYYGTPKPPRQPIIGTVTLSDTGSHQSTPKRTKSYNDMQNVRVVPADDDDDDQATEMNNSFTGTLVRSLFPSPLLCTGNPNDQDESRTGILRPYPVRDNALACGLNNATTSTVESGQQTLAEPQVSPDDPLGPLPENWEMAYTENGELYFIDTAKPHHQDMAVHFQSVHNTKTTSWLDPRCRDKASRPLEECDDDEEGIHTEDLESDLELPPGWERIDDPVYGVYYVDHINRKTQYENPVVEARRQKLLEQQQQPQPPEGERYIREWIEEHSAAAAPLANYAPNHLETYRDPQVPPALPPGPAGIKRGKPFFTRNPAELKGTFINTKLKKSHRGFGFTVVGGDEPDEFLQIKSLVLDGPAAVDGKMETGDVIVSVNDTIVLGYTHAQVVKIFQSIPIGSMVDLALCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETFDTPPNQAGPLNSMREPRPLSPSAEVASNGSHGYSSDVVTLASSIATQPELITIHMEKGDKGFGFTIADSLTGGGQRVKQIVDYPRCRGLKEGDILMEVNKRNVQNMSHNQVVDLLSKCPRGSEVTMLVQRGVVPAKRSPKLVHQLERKDSQNSSQHSVCSHRSTHTDSPSHPPSAMPSEAVVPTPAAPTQPLPVLPPQDPADGMLTLQKKPDPFKIWAQSRSMYESRLPDCQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTPGYGGSKGDGDVPPSPASSHHSSTQAPSLTEGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGSAVVPASLQPYDVEIQRGENEGFGFVIVSSVSRPDAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVSLRIIPGDESSNASLLTNAEKIATITTTHTPHSTETRNNSKSKGAPPPPPSQTQTQEAEFYSVDLERDSKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKSMKHSRAIELIKNGGRRARLVLKRGDGSVPEYDGSSDGHPPTPRAQNTPEVRTLPPSSRYHTSLESSYPPDLHKSSRLEEAACGRGMDKNRDRAGSDQMEFQGRQFNPHHHYTWNNNHSQSTPRQQSPDSNSTSSGNKKTRGRKVKKSESESGISKLLKTLRKDSSGKKAAAAEKLRGRELSSSGSTLDEKYLRQRRGSLDRSPTRKRTSSPDRRRAKSMDRRAERGHRDRTPEREHDDGGFLSVTPERRFHERRLLKDSQIAGRVSEASTPERANNSMESLSRSRGRTDDKATKNGNLLRDSSSERREDRHRMNGTMERRYRAERDSSPDSNYSRDELNYAEAPRLKDYYSMMKNNAAHNNATYNNAGHNNNTVGRSSNQVPEPKRRLYKESPKDLSI

Target ORF information:

RefSeq Version	XM_017409517.1
Organism	Kryptolebias marmoratus(mangrove rivulus)
Definition	Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017409517.1

ORF Insert Sequence:

ATGTCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACGA AAGTGAAGGA GTGCGCCGTG 
GTTCGGGACC CCAGCGGGGA GCTGAACTTG GAGCTGCGCG GCGGGGCGCA GGACGGAGAG 
TTCGCCTACA TCGGACACAT CAGGGAGGAT GCTGTGCTCC ACCAGGACGG GAAACTGTCT 
GAGGGAGAGC TGCTGCTGGA GGTGGAGGGG CTGTCCGTGT CTGGATTACC TCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAAGGT CCCGTCAGGT TCAAAACTGT CCGCCAAGGT 
CACAAGCTCA ACAAGGACCT AAAGCACTTC CTGAGCTTGA GGTTTCAGAA GTCATCACCT 
GACCACGAGT TGCAGAAGAC TATCCGAGCC AATCTATATC GTCATGCCGT GCCTTGTACA 
ACCCGCGCCC CTCGAGACGG AGAGGTGCCA GGGGTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTTGAGGA GAGTGGCACA CTACTGGAAA TAGGAACGTA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC GCCGAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
ACGGGCTCCC ATCAGTCCAC CCCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAACGTC 
CGCGTAGTGC CTGCTGACGA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGAACCT TAGTGCGTAG CCTTTTCCCC TCTCCTTTGT TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGTAC CGGCATCCTT CGGCCATATC CTGTGCGTGA TAATGCTCTG 
GCCTGTGGTC TGAACAATGC CACCACCTCC ACTGTTGAAA GTGGGCAGCA GACGCTCGCA 
GAACCACAGG TCTCTCCAGA TGACCCTCTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACTGAGA ACGGGGAGCT GTACTTCATA GACACTGCAA AGCCTCACCA CCAGGACATG 
GCTGTGCATT TTCAGAGTGT CCACAACACA AAGACCACAT CATGGCTGGA TCCGCGGTGC 
CGAGACAAAG CCTCCAGGCC TTTGGAAGAG TGTGATGATG ATGAAGAAGG GATACATACG 
GAAGACCTGG AAAGTGATCT AGAGCTGCCC CCAGGATGGG AAAGAATAGA TGACCCCGTG 
TACGGAGTGT ACTATGTAGA TCATATAAAC AGGAAGACCC AATATGAGAA CCCAGTGGTG 
GAGGCGCGGC GCCAAAAACT CTTGGAGCAG CAGCAACAGC CGCAGCCTCC AGAAGGTGAG 
CGGTATATTC GAGAGTGGAT AGAGGAGCAC TCAGCTGCAG CAGCTCCTTT GGCAAACTAT 
GCTCCAAACC ACCTGGAGAC CTACAGGGAC CCACAAGTCC CTCCAGCTCT ACCTCCAGGC 
CCCGCTGGCA TCAAAAGAGG GAAACCTTTC TTTACGAGAA ACCCAGCAGA GCTGAAGGGA 
ACCTTCATCA ACACCAAGCT GAAGAAAAGT CACCGTGGAT TCGGGTTCAC CGTGGTGGGA 
GGTGACGAGC CCGACGAGTT CCTGCAGATC AAGAGTCTGG TGCTGGATGG GCCTGCGGCT 
GTTGATGGCA AGATGGAGAC AGGTGATGTG ATTGTCAGCG TGAATGACAC TATTGTGCTG 
GGCTACACTC ATGCTCAGGT GGTGAAGATC TTCCAGTCCA TCCCTATCGG CTCCATGGTG 
GACCTGGCCT TATGCCGTGG CTACCCACTG CCCTTTGACC CAGACGACCC AAACACCAGC 
CTGGTGACCT CTGTGGCCAT CTTGGACAAG GAACCCATTA TTGTCAATGG GCAAGAGACG 
TTTGACACGC CGCCTAACCA GGCTGGACCC CTTAACAGCA TGCGTGAGCC ACGGCCTCTC 
AGCCCCTCAG CTGAGGTGGC GTCCAACGGC TCCCATGGTT ACTCCAGCGA CGTGGTCACG 
TTGGCCTCGT CCATAGCCAC TCAGCCCGAG CTCATCACCA TCCACATGGA GAAGGGGGAC 
AAAGGTTTTG GCTTCACCAT CGCTGACAGC CTAACAGGCG GAGGCCAGAG GGTAAAGCAG 
ATAGTGGACT ATCCACGGTG CCGCGGCCTG AAAGAGGGGG ACATTCTAAT GGAGGTCAAC 
AAGAGAAATG TCCAGAATAT GAGTCACAAT CAGGTGGTGG ATCTGCTGAG CAAATGTCCA 
AGAGGCAGTG AGGTCACCAT GTTGGTGCAA AGAGGAGTGG TGCCAGCCAA GAGGAGCCCA 
AAGCTGGTGC ACCAACTGGA GAGAAAAGAC AGTCAGAACA GCTCCCAGCA CAGTGTGTGC 
AGCCATCGCA GCACCCACAC CGATTCTCCC AGCCACCCAC CCTCTGCCAT GCCCAGTGAG 
GCAGTGGTCC CCACTCCCGC TGCCCCCACT CAGCCTCTGC CAGTCTTGCC CCCCCAGGAC 
CCCGCAGATG GCATGCTCAC CCTCCAGAAG AAGCCAGACC CTTTTAAGAT CTGGGCACAG 
TCCAGGAGCA TGTATGAGAG TCGACTACCA GATTGCCAGG AGCAAGACAT TTTCCTTTGG 
CGGAAAGATA CAGGTTTTGG GTTTCGCATC TTGGGAGGAA ATGAGCCTGG GGAGCCTATA 
TACATAGGGC ACATAGTGAA GTACGGGGCC GCAGATGAAG ATGGCCGCCT GCGATCGGGT 
GACGAGCTCA TCTGTGTGGA CGGCACGGCT GTAGTGGGCA AATCTCACCA GCTGGTGGTG 
CAGCTGATGC AGCAGGCAGC CAAGCAGGGC CACGTCAACC TCACTGTCAG ACGCAAGACC 
CCCGGATACG GAGGGTCAAA AGGAGATGGT GACGTTCCTC CATCCCCAGC CTCCTCCCAC 
CACAGCAGCA CCCAGGCACC CAGTCTGACG GAGGGAAAGC GCACCCCTCA GGGCAGCCAG 
AACTCACTCA ACACTGTCAG CTCCGGCAGC GGATCCACCA GCGGCATAGG CAGCGGCGGT 
GGAGGAGGCA GTGGGAGCGC GGTGGTGCCT GCCTCCCTGC AGCCTTACGA CGTGGAGATC 
CAGCGAGGAG AGAACGAGGG ATTTGGTTTT GTCATCGTGT CGTCCGTTTC CAGGCCTGAT 
GCTGGCACTA CTTTCGCTGG AAATGCTTGT GTGGCAATGC CCCACAAAAT AGGACGCATC 
ATCGAGGGCA GCCCGGCGGA CCGCTGCGGG AAGCTGAAGG TCGGGGACAG AATACTGGCT 
GTTAACGGCT GCTCCATCAC CAACAAGTCT CACTCAGACA TCGTCAACCT GATTAAGGAA 
GCCGGGAACA CCGTCTCACT CAGGATCATC CCAGGTGATG AATCTTCCAA TGCTTCTCTG 
CTGACTAATG CTGAGAAGAT AGCTACCATC ACCACGACAC ACACACCTCA TTCCACGGAG 
ACCAGAAATA ATTCAAAGTC AAAAGGAGCT CCTCCTCCAC CCCCTTCACA GACCCAAACA 
CAGGAAGCAG AGTTTTATTC TGTGGACCTC GAGAGGGACA GTAAAGGCTT CGGCTTCAGC 
TTAAGAGGAG GACGGGAGTA CAACATGGAC CTGTACGTGT TGAGACTAGC AGAGGATGGG 
GCTGCTGTCA GAAATGGCAA GATGAGGGTG GGAGACGAGA TCCTGGAGAT TAATGGTGAG 
AGCACAAAGA GCATGAAGCA TTCACGAGCC ATTGAACTGA TCAAGAACGG CGGTCGGAGG 
GCACGACTTG TCCTAAAAAG GGGCGATGGA TCTGTACCTG AATATGACGG CAGCAGTGAC 
GGGCACCCTC CCACACCCCG GGCACAAAAC ACTCCGGAAG TGAGAACGTT GCCACCCAGC 
AGCCGATATC ACACCTCATT GGAGTCCAGT TATCCACCAG ACCTTCACAA ATCATCACGC 
CTGGAGGAGG CTGCTTGTGG CCGAGGTATG GACAAGAACA GGGACAGGGC TGGTTCAGAT 
CAGATGGAAT TCCAAGGCCG ACAATTTAAC CCCCATCACC ATTACACTTG GAATAATAAT 
CACAGTCAAA GTACCCCCAG ACAGCAGTCC CCTGACAGTA ATAGCACTAG CAGTGGCAAC 
AAGAAGACCA GAGGCCGTAA AGTCAAGAAG TCTGAATCGG AGAGTGGCAT CTCTAAACTT 
CTAAAGACTC TGAGGAAGGA CAGCTCGGGA AAAAAGGCTG CAGCCGCCGA AAAACTAAGG 
GGCCGAGAGC TTTCAAGCAG CGGTTCTACT TTGGATGAGA AGTATCTTCG GCAGCGTCGT 
GGGTCCCTCG ACCGCTCACC AACCAGAAAG CGCACCTCGT CTCCAGATCG TCGACGGGCG 
AAATCCATGG ACCGCAGGGC AGAGCGAGGA CATCGTGACC GCACACCTGA GAGGGAACAC 
GATGATGGAG GGTTTCTCTC AGTCACCCCT GAACGGAGGT TCCACGAGCG AAGGCTCCTC 
AAGGACTCAC AGATAGCTGG ACGAGTCAGT GAGGCTTCAA CCCCCGAGCG CGCCAACAAC 
AGCATGGAAT CCTTGTCTCG TTCCAGGGGC CGCACGGACG ATAAAGCCAC AAAGAACGGC 
AACCTTCTGA GAGACTCTTC TTCAGAGAGG AGAGAGGACC GACATCGCAT GAATGGGACA 
ATGGAGAGGC GATACAGAGC GGAGCGGGAC TCTTCCCCTG ACAGCAACTA CAGCCGGGAC 
GAGCTGAACT ATGCAGAAGC ACCTAGACTG AAGGACTACT ACAGCATGAT GAAGAACAAC 
GCTGCACACA ACAATGCTAC CTACAACAAC GCAGGCCATA ACAATAACAC AGTAGGCCGC 
AGCTCAAACC AGGTCCCTGA GCCCAAGAGG AGGCTGTACA AAGAAAGTCC TAAAGACCTC 
AGCATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108232031 ( XM_017409518.1 ) cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus) -> XP_017265007.1 Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X2, mRNA.

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CloneID	OKr06769
Clone ID Related Accession (Same CDS sequence)	XM_017409518.1 , XM_017409518.2
Accession Version	XM_017409518.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-27
Organism	Kryptolebias marmoratus(mangrove rivulus)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016094253.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Kryptolebias marmoratus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACGA AAGTGAAGGA GTGCGCCGTG 
GTTCGGGACC CCAGCGGGGA GCTGAACTTG GAGCTGCGCG GCGGGGCGCA GGACGGAGAG 
TTCGCCTACA TCGGACACAT CAGGGAGGAT GCTGTGCTCC ACCAGGACGG GAAACTGTCT 
GAGGGAGAGC TGCTGCTGGA GGTGGAGGGG CTGTCCGTGT CTGGATTACC TCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAAGGT CCCGTCAGGT TCAAAACTGT CCGCCAAGGT 
CACAAGCTCA ACAAGGACCT AAAGCACTTC CTGAGCTTGA GGTTTCAGAA GTCATCACCT 
GACCACGAGT TGCAGAAGAC TATCCGAGCC AATCTATATC GTCATGCCGT GCCTTGTACA 
ACCCGCGCCC CTCGAGACGG AGAGGTGCCA GGGGTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTTGAGGA GAGTGGCACA CTACTGGAAA TAGGAACGTA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC GCCGAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
ACGGGCTCCC ATCAGTCCAC CCCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAACGTC 
CGCGTAGTGC CTGCTGACGA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGAACCT TAGTGCGTAG CCTTTTCCCC TCTCCTTTGT TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGTAC CGGCATCCTT CGGCCATATC CTGTGCGTGA TAATGCTCTG 
GCCTGTGGTC TGAACAATGC CACCACCTCC ACTGTTGAAA GTGGGCAGCA GACGCTCGCA 
GAACCACAGG TCTCTCCAGA TGACCCTCTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACTGAGA ACGGGGAGCT GTACTTCATA GACACTGCAA AGCCTCACCA CCAGGACATG 
GCTGTGCATT TTCAGAGTGT CCACAACACA AAGACCACAT CATGGCTGGA TCCGCGGTGC 
CGAGACAAAG CCTCCAGGCC TTTGGAAGAG TGTGATGATG ATGAAGGGAT ACATACGGAA 
GACCTGGAAA GTGATCTAGA GCTGCCCCCA GGATGGGAAA GAATAGATGA CCCCGTGTAC 
GGAGTGTACT ATGTAGATCA TATAAACAGG AAGACCCAAT ATGAGAACCC AGTGGTGGAG 
GCGCGGCGCC AAAAACTCTT GGAGCAGCAG CAACAGCCGC AGCCTCCAGA AGGTGAGCGG 
TATATTCGAG AGTGGATAGA GGAGCACTCA GCTGCAGCAG CTCCTTTGGC AAACTATGCT 
CCAAACCACC TGGAGACCTA CAGGGACCCA CAAGTCCCTC CAGCTCTACC TCCAGGCCCC 
GCTGGCATCA AAAGAGGGAA ACCTTTCTTT ACGAGAAACC CAGCAGAGCT GAAGGGAACC 
TTCATCAACA CCAAGCTGAA GAAAAGTCAC CGTGGATTCG GGTTCACCGT GGTGGGAGGT 
GACGAGCCCG ACGAGTTCCT GCAGATCAAG AGTCTGGTGC TGGATGGGCC TGCGGCTGTT 
GATGGCAAGA TGGAGACAGG TGATGTGATT GTCAGCGTGA ATGACACTAT TGTGCTGGGC 
TACACTCATG CTCAGGTGGT GAAGATCTTC CAGTCCATCC CTATCGGCTC CATGGTGGAC 
CTGGCCTTAT GCCGTGGCTA CCCACTGCCC TTTGACCCAG ACGACCCAAA CACCAGCCTG 
GTGACCTCTG TGGCCATCTT GGACAAGGAA CCCATTATTG TCAATGGGCA AGAGACGTTT 
GACACGCCGC CTAACCAGGC TGGACCCCTT AACAGCATGC GTGAGCCACG GCCTCTCAGC 
CCCTCAGCTG AGGTGGCGTC CAACGGCTCC CATGGTTACT CCAGCGACGT GGTCACGTTG 
GCCTCGTCCA TAGCCACTCA GCCCGAGCTC ATCACCATCC ACATGGAGAA GGGGGACAAA 
GGTTTTGGCT TCACCATCGC TGACAGCCTA ACAGGCGGAG GCCAGAGGGT AAAGCAGATA 
GTGGACTATC CACGGTGCCG CGGCCTGAAA GAGGGGGACA TTCTAATGGA GGTCAACAAG 
AGAAATGTCC AGAATATGAG TCACAATCAG GTGGTGGATC TGCTGAGCAA ATGTCCAAGA 
GGCAGTGAGG TCACCATGTT GGTGCAAAGA GGAGTGGTGC CAGCCAAGAG GAGCCCAAAG 
CTGGTGCACC AACTGGAGAG AAAAGACAGT CAGAACAGCT CCCAGCACAG TGTGTGCAGC 
CATCGCAGCA CCCACACCGA TTCTCCCAGC CACCCACCCT CTGCCATGCC CAGTGAGGCA 
GTGGTCCCCA CTCCCGCTGC CCCCACTCAG CCTCTGCCAG TCTTGCCCCC CCAGGACCCC 
GCAGATGGCA TGCTCACCCT CCAGAAGAAG CCAGACCCTT TTAAGATCTG GGCACAGTCC 
AGGAGCATGT ATGAGAGTCG ACTACCAGAT TGCCAGGAGC AAGACATTTT CCTTTGGCGG 
AAAGATACAG GTTTTGGGTT TCGCATCTTG GGAGGAAATG AGCCTGGGGA GCCTATATAC 
ATAGGGCACA TAGTGAAGTA CGGGGCCGCA GATGAAGATG GCCGCCTGCG ATCGGGTGAC 
GAGCTCATCT GTGTGGACGG CACGGCTGTA GTGGGCAAAT CTCACCAGCT GGTGGTGCAG 
CTGATGCAGC AGGCAGCCAA GCAGGGCCAC GTCAACCTCA CTGTCAGACG CAAGACCCCC 
GGATACGGAG GGTCAAAAGG AGATGGTGAC GTTCCTCCAT CCCCAGCCTC CTCCCACCAC 
AGCAGCACCC AGGCACCCAG TCTGACGGAG GGAAAGCGCA CCCCTCAGGG CAGCCAGAAC 
TCACTCAACA CTGTCAGCTC CGGCAGCGGA TCCACCAGCG GCATAGGCAG CGGCGGTGGA 
GGAGGCAGTG GGAGCGCGGT GGTGCCTGCC TCCCTGCAGC CTTACGACGT GGAGATCCAG 
CGAGGAGAGA ACGAGGGATT TGGTTTTGTC ATCGTGTCGT CCGTTTCCAG GCCTGATGCT 
GGCACTACTT TCGCTGGAAA TGCTTGTGTG GCAATGCCCC ACAAAATAGG ACGCATCATC 
GAGGGCAGCC CGGCGGACCG CTGCGGGAAG CTGAAGGTCG GGGACAGAAT ACTGGCTGTT 
AACGGCTGCT CCATCACCAA CAAGTCTCAC TCAGACATCG TCAACCTGAT TAAGGAAGCC 
GGGAACACCG TCTCACTCAG GATCATCCCA GGTGATGAAT CTTCCAATGC TTCTCTGCTG 
ACTAATGCTG AGAAGATAGC TACCATCACC ACGACACACA CACCTCATTC CACGGAGACC 
AGAAATAATT CAAAGTCAAA AGGAGCTCCT CCTCCACCCC CTTCACAGAC CCAAACACAG 
GAAGCAGAGT TTTATTCTGT GGACCTCGAG AGGGACAGTA AAGGCTTCGG CTTCAGCTTA 
AGAGGAGGAC GGGAGTACAA CATGGACCTG TACGTGTTGA GACTAGCAGA GGATGGGGCT 
GCTGTCAGAA ATGGCAAGAT GAGGGTGGGA GACGAGATCC TGGAGATTAA TGGTGAGAGC 
ACAAAGAGCA TGAAGCATTC ACGAGCCATT GAACTGATCA AGAACGGCGG TCGGAGGGCA 
CGACTTGTCC TAAAAAGGGG CGATGGATCT GTACCTGAAT ATGACGGCAG CAGTGACGGG 
CACCCTCCCA CACCCCGGGC ACAAAACACT CCGGAAGTGA GAACGTTGCC ACCCAGCAGC 
CGATATCACA CCTCATTGGA GTCCAGTTAT CCACCAGACC TTCACAAATC ATCACGCCTG 
GAGGAGGCTG CTTGTGGCCG AGGTATGGAC AAGAACAGGG ACAGGGCTGG TTCAGATCAG 
ATGGAATTCC AAGGCCGACA ATTTAACCCC CATCACCATT ACACTTGGAA TAATAATCAC 
AGTCAAAGTA CCCCCAGACA GCAGTCCCCT GACAGTAATA GCACTAGCAG TGGCAACAAG 
AAGACCAGAG GCCGTAAAGT CAAGAAGTCT GAATCGGAGA GTGGCATCTC TAAACTTCTA 
AAGACTCTGA GGAAGGACAG CTCGGGAAAA AAGGCTGCAG CCGCCGAAAA ACTAAGGGGC 
CGAGAGCTTT CAAGCAGCGG TTCTACTTTG GATGAGAAGT ATCTTCGGCA GCGTCGTGGG 
TCCCTCGACC GCTCACCAAC CAGAAAGCGC ACCTCGTCTC CAGATCGTCG ACGGGCGAAA 
TCCATGGACC GCAGGGCAGA GCGAGGACAT CGTGACCGCA CACCTGAGAG GGAACACGAT 
GATGGAGGGT TTCTCTCAGT CACCCCTGAA CGGAGGTTCC ACGAGCGAAG GCTCCTCAAG 
GACTCACAGA TAGCTGGACG AGTCAGTGAG GCTTCAACCC CCGAGCGCGC CAACAACAGC 
ATGGAATCCT TGTCTCGTTC CAGGGGCCGC ACGGACGATA AAGCCACAAA GAACGGCAAC 
CTTCTGAGAG ACTCTTCTTC AGAGAGGAGA GAGGACCGAC ATCGCATGAA TGGGACAATG 
GAGAGGCGAT ACAGAGCGGA GCGGGACTCT TCCCCTGACA GCAACTACAG CCGGGACGAG 
CTGAACTATG CAGAAGCACC TAGACTGAAG GACTACTACA GCATGATGAA GAACAACGCT 
GCACACAACA ATGCTACCTA CAACAACGCA GGCCATAACA ATAACACAGT AGGCCGCAGC 
TCAAACCAGG TCCCTGAGCC CAAGAGGAGG CTGTACAAAG AAAGTCCTAA AGACCTCAGC 
ATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017265007.1
CDS	775..5640
Translation	MSKVIQKKNHWITKVKECAVVRDPSGELNLELRGGAQDGEFAYIGHIREDAVLHQDGKLSEGELLLEVEGLSVSGLPLYDIYTVINCCKGPVRFKTVRQGHKLNKDLKHFLSLRFQKSSPDHELQKTIRANLYRHAVPCTTRAPRDGEVPGVDYNFLSVEDFLQLEESGTLLEIGTYEGNYYGTPKPPRQPIIGTVTLSDTGSHQSTPKRTKSYNDMQNVRVVPADDDDDDQATEMNNSFTGTLVRSLFPSPLLCTGNPNDQDESRTGILRPYPVRDNALACGLNNATTSTVESGQQTLAEPQVSPDDPLGPLPENWEMAYTENGELYFIDTAKPHHQDMAVHFQSVHNTKTTSWLDPRCRDKASRPLEECDDDEGIHTEDLESDLELPPGWERIDDPVYGVYYVDHINRKTQYENPVVEARRQKLLEQQQQPQPPEGERYIREWIEEHSAAAAPLANYAPNHLETYRDPQVPPALPPGPAGIKRGKPFFTRNPAELKGTFINTKLKKSHRGFGFTVVGGDEPDEFLQIKSLVLDGPAAVDGKMETGDVIVSVNDTIVLGYTHAQVVKIFQSIPIGSMVDLALCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETFDTPPNQAGPLNSMREPRPLSPSAEVASNGSHGYSSDVVTLASSIATQPELITIHMEKGDKGFGFTIADSLTGGGQRVKQIVDYPRCRGLKEGDILMEVNKRNVQNMSHNQVVDLLSKCPRGSEVTMLVQRGVVPAKRSPKLVHQLERKDSQNSSQHSVCSHRSTHTDSPSHPPSAMPSEAVVPTPAAPTQPLPVLPPQDPADGMLTLQKKPDPFKIWAQSRSMYESRLPDCQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTPGYGGSKGDGDVPPSPASSHHSSTQAPSLTEGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGSAVVPASLQPYDVEIQRGENEGFGFVIVSSVSRPDAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVSLRIIPGDESSNASLLTNAEKIATITTTHTPHSTETRNNSKSKGAPPPPPSQTQTQEAEFYSVDLERDSKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKSMKHSRAIELIKNGGRRARLVLKRGDGSVPEYDGSSDGHPPTPRAQNTPEVRTLPPSSRYHTSLESSYPPDLHKSSRLEEAACGRGMDKNRDRAGSDQMEFQGRQFNPHHHYTWNNNHSQSTPRQQSPDSNSTSSGNKKTRGRKVKKSESESGISKLLKTLRKDSSGKKAAAAEKLRGRELSSSGSTLDEKYLRQRRGSLDRSPTRKRTSSPDRRRAKSMDRRAERGHRDRTPEREHDDGGFLSVTPERRFHERRLLKDSQIAGRVSEASTPERANNSMESLSRSRGRTDDKATKNGNLLRDSSSERREDRHRMNGTMERRYRAERDSSPDSNYSRDELNYAEAPRLKDYYSMMKNNAAHNNATYNNAGHNNNTVGRSSNQVPEPKRRLYKESPKDLSI

Target ORF information:

RefSeq Version	XM_017409518.1
Organism	Kryptolebias marmoratus(mangrove rivulus)
Definition	Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017409518.1

ORF Insert Sequence:

ATGTCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACGA AAGTGAAGGA GTGCGCCGTG 
GTTCGGGACC CCAGCGGGGA GCTGAACTTG GAGCTGCGCG GCGGGGCGCA GGACGGAGAG 
TTCGCCTACA TCGGACACAT CAGGGAGGAT GCTGTGCTCC ACCAGGACGG GAAACTGTCT 
GAGGGAGAGC TGCTGCTGGA GGTGGAGGGG CTGTCCGTGT CTGGATTACC TCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAAGGT CCCGTCAGGT TCAAAACTGT CCGCCAAGGT 
CACAAGCTCA ACAAGGACCT AAAGCACTTC CTGAGCTTGA GGTTTCAGAA GTCATCACCT 
GACCACGAGT TGCAGAAGAC TATCCGAGCC AATCTATATC GTCATGCCGT GCCTTGTACA 
ACCCGCGCCC CTCGAGACGG AGAGGTGCCA GGGGTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTTGAGGA GAGTGGCACA CTACTGGAAA TAGGAACGTA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC GCCGAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
ACGGGCTCCC ATCAGTCCAC CCCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAACGTC 
CGCGTAGTGC CTGCTGACGA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGAACCT TAGTGCGTAG CCTTTTCCCC TCTCCTTTGT TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGTAC CGGCATCCTT CGGCCATATC CTGTGCGTGA TAATGCTCTG 
GCCTGTGGTC TGAACAATGC CACCACCTCC ACTGTTGAAA GTGGGCAGCA GACGCTCGCA 
GAACCACAGG TCTCTCCAGA TGACCCTCTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACTGAGA ACGGGGAGCT GTACTTCATA GACACTGCAA AGCCTCACCA CCAGGACATG 
GCTGTGCATT TTCAGAGTGT CCACAACACA AAGACCACAT CATGGCTGGA TCCGCGGTGC 
CGAGACAAAG CCTCCAGGCC TTTGGAAGAG TGTGATGATG ATGAAGGGAT ACATACGGAA 
GACCTGGAAA GTGATCTAGA GCTGCCCCCA GGATGGGAAA GAATAGATGA CCCCGTGTAC 
GGAGTGTACT ATGTAGATCA TATAAACAGG AAGACCCAAT ATGAGAACCC AGTGGTGGAG 
GCGCGGCGCC AAAAACTCTT GGAGCAGCAG CAACAGCCGC AGCCTCCAGA AGGTGAGCGG 
TATATTCGAG AGTGGATAGA GGAGCACTCA GCTGCAGCAG CTCCTTTGGC AAACTATGCT 
CCAAACCACC TGGAGACCTA CAGGGACCCA CAAGTCCCTC CAGCTCTACC TCCAGGCCCC 
GCTGGCATCA AAAGAGGGAA ACCTTTCTTT ACGAGAAACC CAGCAGAGCT GAAGGGAACC 
TTCATCAACA CCAAGCTGAA GAAAAGTCAC CGTGGATTCG GGTTCACCGT GGTGGGAGGT 
GACGAGCCCG ACGAGTTCCT GCAGATCAAG AGTCTGGTGC TGGATGGGCC TGCGGCTGTT 
GATGGCAAGA TGGAGACAGG TGATGTGATT GTCAGCGTGA ATGACACTAT TGTGCTGGGC 
TACACTCATG CTCAGGTGGT GAAGATCTTC CAGTCCATCC CTATCGGCTC CATGGTGGAC 
CTGGCCTTAT GCCGTGGCTA CCCACTGCCC TTTGACCCAG ACGACCCAAA CACCAGCCTG 
GTGACCTCTG TGGCCATCTT GGACAAGGAA CCCATTATTG TCAATGGGCA AGAGACGTTT 
GACACGCCGC CTAACCAGGC TGGACCCCTT AACAGCATGC GTGAGCCACG GCCTCTCAGC 
CCCTCAGCTG AGGTGGCGTC CAACGGCTCC CATGGTTACT CCAGCGACGT GGTCACGTTG 
GCCTCGTCCA TAGCCACTCA GCCCGAGCTC ATCACCATCC ACATGGAGAA GGGGGACAAA 
GGTTTTGGCT TCACCATCGC TGACAGCCTA ACAGGCGGAG GCCAGAGGGT AAAGCAGATA 
GTGGACTATC CACGGTGCCG CGGCCTGAAA GAGGGGGACA TTCTAATGGA GGTCAACAAG 
AGAAATGTCC AGAATATGAG TCACAATCAG GTGGTGGATC TGCTGAGCAA ATGTCCAAGA 
GGCAGTGAGG TCACCATGTT GGTGCAAAGA GGAGTGGTGC CAGCCAAGAG GAGCCCAAAG 
CTGGTGCACC AACTGGAGAG AAAAGACAGT CAGAACAGCT CCCAGCACAG TGTGTGCAGC 
CATCGCAGCA CCCACACCGA TTCTCCCAGC CACCCACCCT CTGCCATGCC CAGTGAGGCA 
GTGGTCCCCA CTCCCGCTGC CCCCACTCAG CCTCTGCCAG TCTTGCCCCC CCAGGACCCC 
GCAGATGGCA TGCTCACCCT CCAGAAGAAG CCAGACCCTT TTAAGATCTG GGCACAGTCC 
AGGAGCATGT ATGAGAGTCG ACTACCAGAT TGCCAGGAGC AAGACATTTT CCTTTGGCGG 
AAAGATACAG GTTTTGGGTT TCGCATCTTG GGAGGAAATG AGCCTGGGGA GCCTATATAC 
ATAGGGCACA TAGTGAAGTA CGGGGCCGCA GATGAAGATG GCCGCCTGCG ATCGGGTGAC 
GAGCTCATCT GTGTGGACGG CACGGCTGTA GTGGGCAAAT CTCACCAGCT GGTGGTGCAG 
CTGATGCAGC AGGCAGCCAA GCAGGGCCAC GTCAACCTCA CTGTCAGACG CAAGACCCCC 
GGATACGGAG GGTCAAAAGG AGATGGTGAC GTTCCTCCAT CCCCAGCCTC CTCCCACCAC 
AGCAGCACCC AGGCACCCAG TCTGACGGAG GGAAAGCGCA CCCCTCAGGG CAGCCAGAAC 
TCACTCAACA CTGTCAGCTC CGGCAGCGGA TCCACCAGCG GCATAGGCAG CGGCGGTGGA 
GGAGGCAGTG GGAGCGCGGT GGTGCCTGCC TCCCTGCAGC CTTACGACGT GGAGATCCAG 
CGAGGAGAGA ACGAGGGATT TGGTTTTGTC ATCGTGTCGT CCGTTTCCAG GCCTGATGCT 
GGCACTACTT TCGCTGGAAA TGCTTGTGTG GCAATGCCCC ACAAAATAGG ACGCATCATC 
GAGGGCAGCC CGGCGGACCG CTGCGGGAAG CTGAAGGTCG GGGACAGAAT ACTGGCTGTT 
AACGGCTGCT CCATCACCAA CAAGTCTCAC TCAGACATCG TCAACCTGAT TAAGGAAGCC 
GGGAACACCG TCTCACTCAG GATCATCCCA GGTGATGAAT CTTCCAATGC TTCTCTGCTG 
ACTAATGCTG AGAAGATAGC TACCATCACC ACGACACACA CACCTCATTC CACGGAGACC 
AGAAATAATT CAAAGTCAAA AGGAGCTCCT CCTCCACCCC CTTCACAGAC CCAAACACAG 
GAAGCAGAGT TTTATTCTGT GGACCTCGAG AGGGACAGTA AAGGCTTCGG CTTCAGCTTA 
AGAGGAGGAC GGGAGTACAA CATGGACCTG TACGTGTTGA GACTAGCAGA GGATGGGGCT 
GCTGTCAGAA ATGGCAAGAT GAGGGTGGGA GACGAGATCC TGGAGATTAA TGGTGAGAGC 
ACAAAGAGCA TGAAGCATTC ACGAGCCATT GAACTGATCA AGAACGGCGG TCGGAGGGCA 
CGACTTGTCC TAAAAAGGGG CGATGGATCT GTACCTGAAT ATGACGGCAG CAGTGACGGG 
CACCCTCCCA CACCCCGGGC ACAAAACACT CCGGAAGTGA GAACGTTGCC ACCCAGCAGC 
CGATATCACA CCTCATTGGA GTCCAGTTAT CCACCAGACC TTCACAAATC ATCACGCCTG 
GAGGAGGCTG CTTGTGGCCG AGGTATGGAC AAGAACAGGG ACAGGGCTGG TTCAGATCAG 
ATGGAATTCC AAGGCCGACA ATTTAACCCC CATCACCATT ACACTTGGAA TAATAATCAC 
AGTCAAAGTA CCCCCAGACA GCAGTCCCCT GACAGTAATA GCACTAGCAG TGGCAACAAG 
AAGACCAGAG GCCGTAAAGT CAAGAAGTCT GAATCGGAGA GTGGCATCTC TAAACTTCTA 
AAGACTCTGA GGAAGGACAG CTCGGGAAAA AAGGCTGCAG CCGCCGAAAA ACTAAGGGGC 
CGAGAGCTTT CAAGCAGCGG TTCTACTTTG GATGAGAAGT ATCTTCGGCA GCGTCGTGGG 
TCCCTCGACC GCTCACCAAC CAGAAAGCGC ACCTCGTCTC CAGATCGTCG ACGGGCGAAA 
TCCATGGACC GCAGGGCAGA GCGAGGACAT CGTGACCGCA CACCTGAGAG GGAACACGAT 
GATGGAGGGT TTCTCTCAGT CACCCCTGAA CGGAGGTTCC ACGAGCGAAG GCTCCTCAAG 
GACTCACAGA TAGCTGGACG AGTCAGTGAG GCTTCAACCC CCGAGCGCGC CAACAACAGC 
ATGGAATCCT TGTCTCGTTC CAGGGGCCGC ACGGACGATA AAGCCACAAA GAACGGCAAC 
CTTCTGAGAG ACTCTTCTTC AGAGAGGAGA GAGGACCGAC ATCGCATGAA TGGGACAATG 
GAGAGGCGAT ACAGAGCGGA GCGGGACTCT TCCCCTGACA GCAACTACAG CCGGGACGAG 
CTGAACTATG CAGAAGCACC TAGACTGAAG GACTACTACA GCATGATGAA GAACAACGCT 
GCACACAACA ATGCTACCTA CAACAACGCA GGCCATAACA ATAACACAGT AGGCCGCAGC 
TCAAACCAGG TCCCTGAGCC CAAGAGGAGG CTGTACAAAG AAAGTCCTAA AGACCTCAGC 
ATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108232031 ( XM_017409518.2 ) cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus) -> XP_017265007.1 Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X2, mRNA.

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CloneID	OKr06769
Clone ID Related Accession (Same CDS sequence)	XM_017409518.1 , XM_017409518.2
Accession Version	XM_017409518.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4866bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-03
Organism	Kryptolebias marmoratus(mangrove rivulus)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016094253.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 4, 2018 this sequence version replaced XM_017409518.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Kryptolebias marmoratus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACGA AAGTGAAGGA GTGCGCCGTG 
GTTCGGGACC CCAGCGGGGA GCTGAACTTG GAGCTGCGCG GCGGGGCGCA GGACGGAGAG 
TTCGCCTACA TCGGACACAT CAGGGAGGAT GCTGTGCTCC ACCAGGACGG GAAACTGTCT 
GAGGGAGAGC TGCTGCTGGA GGTGGAGGGG CTGTCCGTGT CTGGATTACC TCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAAGGT CCCGTCAGGT TCAAAACTGT CCGCCAAGGT 
CACAAGCTCA ACAAGGACCT AAAGCACTTC CTGAGCTTGA GGTTTCAGAA GTCATCACCT 
GACCACGAGT TGCAGAAGAC TATCCGAGCC AATCTATATC GTCATGCCGT GCCTTGTACA 
ACCCGCGCCC CTCGAGACGG AGAGGTGCCA GGGGTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTTGAGGA GAGTGGCACA CTACTGGAAA TAGGAACGTA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC GCCGAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
ACGGGCTCCC ATCAGTCCAC CCCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAACGTC 
CGCGTAGTGC CTGCTGACGA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGAACCT TAGTGCGTAG CCTTTTCCCC TCTCCTTTGT TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGTAC CGGCATCCTT CGGCCATATC CTGTGCGTGA TAATGCTCTG 
GCCTGTGGTC TGAACAATGC CACCACCTCC ACTGTTGAAA GTGGGCAGCA GACGCTCGCA 
GAACCACAGG TCTCTCCAGA TGACCCTCTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACTGAGA ACGGGGAGCT GTACTTCATA GACACTGCAA AGCCTCACCA CCAGGACATG 
GCTGTGCATT TTCAGAGTGT CCACAACACA AAGACCACAT CATGGCTGGA TCCGCGGTGC 
CGAGACAAAG CCTCCAGGCC TTTGGAAGAG TGTGATGATG ATGAAGGGAT ACATACGGAA 
GACCTGGAAA GTGATCTAGA GCTGCCCCCA GGATGGGAAA GAATAGATGA CCCCGTGTAC 
GGAGTGTACT ATGTAGATCA TATAAACAGG AAGACCCAAT ATGAGAACCC AGTGGTGGAG 
GCGCGGCGCC AAAAACTCTT GGAGCAGCAG CAACAGCCGC AGCCTCCAGA AGGTGAGCGG 
TATATTCGAG AGTGGATAGA GGAGCACTCA GCTGCAGCAG CTCCTTTGGC AAACTATGCT 
CCAAACCACC TGGAGACCTA CAGGGACCCA CAAGTCCCTC CAGCTCTACC TCCAGGCCCC 
GCTGGCATCA AAAGAGGGAA ACCTTTCTTT ACGAGAAACC CAGCAGAGCT GAAGGGAACC 
TTCATCAACA CCAAGCTGAA GAAAAGTCAC CGTGGATTCG GGTTCACCGT GGTGGGAGGT 
GACGAGCCCG ACGAGTTCCT GCAGATCAAG AGTCTGGTGC TGGATGGGCC TGCGGCTGTT 
GATGGCAAGA TGGAGACAGG TGATGTGATT GTCAGCGTGA ATGACACTAT TGTGCTGGGC 
TACACTCATG CTCAGGTGGT GAAGATCTTC CAGTCCATCC CTATCGGCTC CATGGTGGAC 
CTGGCCTTAT GCCGTGGCTA CCCACTGCCC TTTGACCCAG ACGACCCAAA CACCAGCCTG 
GTGACCTCTG TGGCCATCTT GGACAAGGAA CCCATTATTG TCAATGGGCA AGAGACGTTT 
GACACGCCGC CTAACCAGGC TGGACCCCTT AACAGCATGC GTGAGCCACG GCCTCTCAGC 
CCCTCAGCTG AGGTGGCGTC CAACGGCTCC CATGGTTACT CCAGCGACGT GGTCACGTTG 
GCCTCGTCCA TAGCCACTCA GCCCGAGCTC ATCACCATCC ACATGGAGAA GGGGGACAAA 
GGTTTTGGCT TCACCATCGC TGACAGCCTA ACAGGCGGAG GCCAGAGGGT AAAGCAGATA 
GTGGACTATC CACGGTGCCG CGGCCTGAAA GAGGGGGACA TTCTAATGGA GGTCAACAAG 
AGAAATGTCC AGAATATGAG TCACAATCAG GTGGTGGATC TGCTGAGCAA ATGTCCAAGA 
GGCAGTGAGG TCACCATGTT GGTGCAAAGA GGAGTGGTGC CAGCCAAGAG GAGCCCAAAG 
CTGGTGCACC AACTGGAGAG AAAAGACAGT CAGAACAGCT CCCAGCACAG TGTGTGCAGC 
CATCGCAGCA CCCACACCGA TTCTCCCAGC CACCCACCCT CTGCCATGCC CAGTGAGGCA 
GTGGTCCCCA CTCCCGCTGC CCCCACTCAG CCTCTGCCAG TCTTGCCCCC CCAGGACCCC 
GCAGATGGCA TGCTCACCCT CCAGAAGAAG CCAGACCCTT TTAAGATCTG GGCACAGTCC 
AGGAGCATGT ATGAGAGTCG ACTACCAGAT TGCCAGGAGC AAGACATTTT CCTTTGGCGG 
AAAGATACAG GTTTTGGGTT TCGCATCTTG GGAGGAAATG AGCCTGGGGA GCCTATATAC 
ATAGGGCACA TAGTGAAGTA CGGGGCCGCA GATGAAGATG GCCGCCTGCG ATCGGGTGAC 
GAGCTCATCT GTGTGGACGG CACGGCTGTA GTGGGCAAAT CTCACCAGCT GGTGGTGCAG 
CTGATGCAGC AGGCAGCCAA GCAGGGCCAC GTCAACCTCA CTGTCAGACG CAAGACCCCC 
GGATACGGAG GGTCAAAAGG AGATGGTGAC GTTCCTCCAT CCCCAGCCTC CTCCCACCAC 
AGCAGCACCC AGGCACCCAG TCTGACGGAG GGAAAGCGCA CCCCTCAGGG CAGCCAGAAC 
TCACTCAACA CTGTCAGCTC CGGCAGCGGA TCCACCAGCG GCATAGGCAG CGGCGGTGGA 
GGAGGCAGTG GGAGCGCGGT GGTGCCTGCC TCCCTGCAGC CTTACGACGT GGAGATCCAG 
CGAGGAGAGA ACGAGGGATT TGGTTTTGTC ATCGTGTCGT CCGTTTCCAG GCCTGATGCT 
GGCACTACTT TCGCTGGAAA TGCTTGTGTG GCAATGCCCC ACAAAATAGG ACGCATCATC 
GAGGGCAGCC CGGCGGACCG CTGCGGGAAG CTGAAGGTCG GGGACAGAAT ACTGGCTGTT 
AACGGCTGCT CCATCACCAA CAAGTCTCAC TCAGACATCG TCAACCTGAT TAAGGAAGCC 
GGGAACACCG TCTCACTCAG GATCATCCCA GGTGATGAAT CTTCCAATGC TTCTCTGCTG 
ACTAATGCTG AGAAGATAGC TACCATCACC ACGACACACA CACCTCATTC CACGGAGACC 
AGAAATAATT CAAAGTCAAA AGGAGCTCCT CCTCCACCCC CTTCACAGAC CCAAACACAG 
GAAGCAGAGT TTTATTCTGT GGACCTCGAG AGGGACAGTA AAGGCTTCGG CTTCAGCTTA 
AGAGGAGGAC GGGAGTACAA CATGGACCTG TACGTGTTGA GACTAGCAGA GGATGGGGCT 
GCTGTCAGAA ATGGCAAGAT GAGGGTGGGA GACGAGATCC TGGAGATTAA TGGTGAGAGC 
ACAAAGAGCA TGAAGCATTC ACGAGCCATT GAACTGATCA AGAACGGCGG TCGGAGGGCA 
CGACTTGTCC TAAAAAGGGG CGATGGATCT GTACCTGAAT ATGACGGCAG CAGTGACGGG 
CACCCTCCCA CACCCCGGGC ACAAAACACT CCGGAAGTGA GAACGTTGCC ACCCAGCAGC 
CGATATCACA CCTCATTGGA GTCCAGTTAT CCACCAGACC TTCACAAATC ATCACGCCTG 
GAGGAGGCTG CTTGTGGCCG AGGTATGGAC AAGAACAGGG ACAGGGCTGG TTCAGATCAG 
ATGGAATTCC AAGGCCGACA ATTTAACCCC CATCACCATT ACACTTGGAA TAATAATCAC 
AGTCAAAGTA CCCCCAGACA GCAGTCCCCT GACAGTAATA GCACTAGCAG TGGCAACAAG 
AAGACCAGAG GCCGTAAAGT CAAGAAGTCT GAATCGGAGA GTGGCATCTC TAAACTTCTA 
AAGACTCTGA GGAAGGACAG CTCGGGAAAA AAGGCTGCAG CCGCCGAAAA ACTAAGGGGC 
CGAGAGCTTT CAAGCAGCGG TTCTACTTTG GATGAGAAGT ATCTTCGGCA GCGTCGTGGG 
TCCCTCGACC GCTCACCAAC CAGAAAGCGC ACCTCGTCTC CAGATCGTCG ACGGGCGAAA 
TCCATGGACC GCAGGGCAGA GCGAGGACAT CGTGACCGCA CACCTGAGAG GGAACACGAT 
GATGGAGGGT TTCTCTCAGT CACCCCTGAA CGGAGGTTCC ACGAGCGAAG GCTCCTCAAG 
GACTCACAGA TAGCTGGACG AGTCAGTGAG GCTTCAACCC CCGAGCGCGC CAACAACAGC 
ATGGAATCCT TGTCTCGTTC CAGGGGCCGC ACGGACGATA AAGCCACAAA GAACGGCAAC 
CTTCTGAGAG ACTCTTCTTC AGAGAGGAGA GAGGACCGAC ATCGCATGAA TGGGACAATG 
GAGAGGCGAT ACAGAGCGGA GCGGGACTCT TCCCCTGACA GCAACTACAG CCGGGACGAG 
CTGAACTATG CAGAAGCACC TAGACTGAAG GACTACTACA GCATGATGAA GAACAACGCT 
GCACACAACA ATGCTACCTA CAACAACGCA GGCCATAACA ATAACACAGT AGGCCGCAGC 
TCAAACCAGG TCCCTGAGCC CAAGAGGAGG CTGTACAAAG AAAGTCCTAA AGACCTCAGC 
ATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017265007.1
CDS	893..5758
Translation	MSKVIQKKNHWITKVKECAVVRDPSGELNLELRGGAQDGEFAYIGHIREDAVLHQDGKLSEGELLLEVEGLSVSGLPLYDIYTVINCCKGPVRFKTVRQGHKLNKDLKHFLSLRFQKSSPDHELQKTIRANLYRHAVPCTTRAPRDGEVPGVDYNFLSVEDFLQLEESGTLLEIGTYEGNYYGTPKPPRQPIIGTVTLSDTGSHQSTPKRTKSYNDMQNVRVVPADDDDDDQATEMNNSFTGTLVRSLFPSPLLCTGNPNDQDESRTGILRPYPVRDNALACGLNNATTSTVESGQQTLAEPQVSPDDPLGPLPENWEMAYTENGELYFIDTAKPHHQDMAVHFQSVHNTKTTSWLDPRCRDKASRPLEECDDDEGIHTEDLESDLELPPGWERIDDPVYGVYYVDHINRKTQYENPVVEARRQKLLEQQQQPQPPEGERYIREWIEEHSAAAAPLANYAPNHLETYRDPQVPPALPPGPAGIKRGKPFFTRNPAELKGTFINTKLKKSHRGFGFTVVGGDEPDEFLQIKSLVLDGPAAVDGKMETGDVIVSVNDTIVLGYTHAQVVKIFQSIPIGSMVDLALCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETFDTPPNQAGPLNSMREPRPLSPSAEVASNGSHGYSSDVVTLASSIATQPELITIHMEKGDKGFGFTIADSLTGGGQRVKQIVDYPRCRGLKEGDILMEVNKRNVQNMSHNQVVDLLSKCPRGSEVTMLVQRGVVPAKRSPKLVHQLERKDSQNSSQHSVCSHRSTHTDSPSHPPSAMPSEAVVPTPAAPTQPLPVLPPQDPADGMLTLQKKPDPFKIWAQSRSMYESRLPDCQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTPGYGGSKGDGDVPPSPASSHHSSTQAPSLTEGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGSAVVPASLQPYDVEIQRGENEGFGFVIVSSVSRPDAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVSLRIIPGDESSNASLLTNAEKIATITTTHTPHSTETRNNSKSKGAPPPPPSQTQTQEAEFYSVDLERDSKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKSMKHSRAIELIKNGGRRARLVLKRGDGSVPEYDGSSDGHPPTPRAQNTPEVRTLPPSSRYHTSLESSYPPDLHKSSRLEEAACGRGMDKNRDRAGSDQMEFQGRQFNPHHHYTWNNNHSQSTPRQQSPDSNSTSSGNKKTRGRKVKKSESESGISKLLKTLRKDSSGKKAAAAEKLRGRELSSSGSTLDEKYLRQRRGSLDRSPTRKRTSSPDRRRAKSMDRRAERGHRDRTPEREHDDGGFLSVTPERRFHERRLLKDSQIAGRVSEASTPERANNSMESLSRSRGRTDDKATKNGNLLRDSSSERREDRHRMNGTMERRYRAERDSSPDSNYSRDELNYAEAPRLKDYYSMMKNNAAHNNATYNNAGHNNNTVGRSSNQVPEPKRRLYKESPKDLSI

Target ORF information:

RefSeq Version	XM_017409518.2
Organism	Kryptolebias marmoratus(mangrove rivulus)
Definition	Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017409518.2

ORF Insert Sequence:

ATGTCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACGA AAGTGAAGGA GTGCGCCGTG 
GTTCGGGACC CCAGCGGGGA GCTGAACTTG GAGCTGCGCG GCGGGGCGCA GGACGGAGAG 
TTCGCCTACA TCGGACACAT CAGGGAGGAT GCTGTGCTCC ACCAGGACGG GAAACTGTCT 
GAGGGAGAGC TGCTGCTGGA GGTGGAGGGG CTGTCCGTGT CTGGATTACC TCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAAGGT CCCGTCAGGT TCAAAACTGT CCGCCAAGGT 
CACAAGCTCA ACAAGGACCT AAAGCACTTC CTGAGCTTGA GGTTTCAGAA GTCATCACCT 
GACCACGAGT TGCAGAAGAC TATCCGAGCC AATCTATATC GTCATGCCGT GCCTTGTACA 
ACCCGCGCCC CTCGAGACGG AGAGGTGCCA GGGGTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTTGAGGA GAGTGGCACA CTACTGGAAA TAGGAACGTA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC GCCGAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
ACGGGCTCCC ATCAGTCCAC CCCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAACGTC 
CGCGTAGTGC CTGCTGACGA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGAACCT TAGTGCGTAG CCTTTTCCCC TCTCCTTTGT TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGTAC CGGCATCCTT CGGCCATATC CTGTGCGTGA TAATGCTCTG 
GCCTGTGGTC TGAACAATGC CACCACCTCC ACTGTTGAAA GTGGGCAGCA GACGCTCGCA 
GAACCACAGG TCTCTCCAGA TGACCCTCTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACTGAGA ACGGGGAGCT GTACTTCATA GACACTGCAA AGCCTCACCA CCAGGACATG 
GCTGTGCATT TTCAGAGTGT CCACAACACA AAGACCACAT CATGGCTGGA TCCGCGGTGC 
CGAGACAAAG CCTCCAGGCC TTTGGAAGAG TGTGATGATG ATGAAGGGAT ACATACGGAA 
GACCTGGAAA GTGATCTAGA GCTGCCCCCA GGATGGGAAA GAATAGATGA CCCCGTGTAC 
GGAGTGTACT ATGTAGATCA TATAAACAGG AAGACCCAAT ATGAGAACCC AGTGGTGGAG 
GCGCGGCGCC AAAAACTCTT GGAGCAGCAG CAACAGCCGC AGCCTCCAGA AGGTGAGCGG 
TATATTCGAG AGTGGATAGA GGAGCACTCA GCTGCAGCAG CTCCTTTGGC AAACTATGCT 
CCAAACCACC TGGAGACCTA CAGGGACCCA CAAGTCCCTC CAGCTCTACC TCCAGGCCCC 
GCTGGCATCA AAAGAGGGAA ACCTTTCTTT ACGAGAAACC CAGCAGAGCT GAAGGGAACC 
TTCATCAACA CCAAGCTGAA GAAAAGTCAC CGTGGATTCG GGTTCACCGT GGTGGGAGGT 
GACGAGCCCG ACGAGTTCCT GCAGATCAAG AGTCTGGTGC TGGATGGGCC TGCGGCTGTT 
GATGGCAAGA TGGAGACAGG TGATGTGATT GTCAGCGTGA ATGACACTAT TGTGCTGGGC 
TACACTCATG CTCAGGTGGT GAAGATCTTC CAGTCCATCC CTATCGGCTC CATGGTGGAC 
CTGGCCTTAT GCCGTGGCTA CCCACTGCCC TTTGACCCAG ACGACCCAAA CACCAGCCTG 
GTGACCTCTG TGGCCATCTT GGACAAGGAA CCCATTATTG TCAATGGGCA AGAGACGTTT 
GACACGCCGC CTAACCAGGC TGGACCCCTT AACAGCATGC GTGAGCCACG GCCTCTCAGC 
CCCTCAGCTG AGGTGGCGTC CAACGGCTCC CATGGTTACT CCAGCGACGT GGTCACGTTG 
GCCTCGTCCA TAGCCACTCA GCCCGAGCTC ATCACCATCC ACATGGAGAA GGGGGACAAA 
GGTTTTGGCT TCACCATCGC TGACAGCCTA ACAGGCGGAG GCCAGAGGGT AAAGCAGATA 
GTGGACTATC CACGGTGCCG CGGCCTGAAA GAGGGGGACA TTCTAATGGA GGTCAACAAG 
AGAAATGTCC AGAATATGAG TCACAATCAG GTGGTGGATC TGCTGAGCAA ATGTCCAAGA 
GGCAGTGAGG TCACCATGTT GGTGCAAAGA GGAGTGGTGC CAGCCAAGAG GAGCCCAAAG 
CTGGTGCACC AACTGGAGAG AAAAGACAGT CAGAACAGCT CCCAGCACAG TGTGTGCAGC 
CATCGCAGCA CCCACACCGA TTCTCCCAGC CACCCACCCT CTGCCATGCC CAGTGAGGCA 
GTGGTCCCCA CTCCCGCTGC CCCCACTCAG CCTCTGCCAG TCTTGCCCCC CCAGGACCCC 
GCAGATGGCA TGCTCACCCT CCAGAAGAAG CCAGACCCTT TTAAGATCTG GGCACAGTCC 
AGGAGCATGT ATGAGAGTCG ACTACCAGAT TGCCAGGAGC AAGACATTTT CCTTTGGCGG 
AAAGATACAG GTTTTGGGTT TCGCATCTTG GGAGGAAATG AGCCTGGGGA GCCTATATAC 
ATAGGGCACA TAGTGAAGTA CGGGGCCGCA GATGAAGATG GCCGCCTGCG ATCGGGTGAC 
GAGCTCATCT GTGTGGACGG CACGGCTGTA GTGGGCAAAT CTCACCAGCT GGTGGTGCAG 
CTGATGCAGC AGGCAGCCAA GCAGGGCCAC GTCAACCTCA CTGTCAGACG CAAGACCCCC 
GGATACGGAG GGTCAAAAGG AGATGGTGAC GTTCCTCCAT CCCCAGCCTC CTCCCACCAC 
AGCAGCACCC AGGCACCCAG TCTGACGGAG GGAAAGCGCA CCCCTCAGGG CAGCCAGAAC 
TCACTCAACA CTGTCAGCTC CGGCAGCGGA TCCACCAGCG GCATAGGCAG CGGCGGTGGA 
GGAGGCAGTG GGAGCGCGGT GGTGCCTGCC TCCCTGCAGC CTTACGACGT GGAGATCCAG 
CGAGGAGAGA ACGAGGGATT TGGTTTTGTC ATCGTGTCGT CCGTTTCCAG GCCTGATGCT 
GGCACTACTT TCGCTGGAAA TGCTTGTGTG GCAATGCCCC ACAAAATAGG ACGCATCATC 
GAGGGCAGCC CGGCGGACCG CTGCGGGAAG CTGAAGGTCG GGGACAGAAT ACTGGCTGTT 
AACGGCTGCT CCATCACCAA CAAGTCTCAC TCAGACATCG TCAACCTGAT TAAGGAAGCC 
GGGAACACCG TCTCACTCAG GATCATCCCA GGTGATGAAT CTTCCAATGC TTCTCTGCTG 
ACTAATGCTG AGAAGATAGC TACCATCACC ACGACACACA CACCTCATTC CACGGAGACC 
AGAAATAATT CAAAGTCAAA AGGAGCTCCT CCTCCACCCC CTTCACAGAC CCAAACACAG 
GAAGCAGAGT TTTATTCTGT GGACCTCGAG AGGGACAGTA AAGGCTTCGG CTTCAGCTTA 
AGAGGAGGAC GGGAGTACAA CATGGACCTG TACGTGTTGA GACTAGCAGA GGATGGGGCT 
GCTGTCAGAA ATGGCAAGAT GAGGGTGGGA GACGAGATCC TGGAGATTAA TGGTGAGAGC 
ACAAAGAGCA TGAAGCATTC ACGAGCCATT GAACTGATCA AGAACGGCGG TCGGAGGGCA 
CGACTTGTCC TAAAAAGGGG CGATGGATCT GTACCTGAAT ATGACGGCAG CAGTGACGGG 
CACCCTCCCA CACCCCGGGC ACAAAACACT CCGGAAGTGA GAACGTTGCC ACCCAGCAGC 
CGATATCACA CCTCATTGGA GTCCAGTTAT CCACCAGACC TTCACAAATC ATCACGCCTG 
GAGGAGGCTG CTTGTGGCCG AGGTATGGAC AAGAACAGGG ACAGGGCTGG TTCAGATCAG 
ATGGAATTCC AAGGCCGACA ATTTAACCCC CATCACCATT ACACTTGGAA TAATAATCAC 
AGTCAAAGTA CCCCCAGACA GCAGTCCCCT GACAGTAATA GCACTAGCAG TGGCAACAAG 
AAGACCAGAG GCCGTAAAGT CAAGAAGTCT GAATCGGAGA GTGGCATCTC TAAACTTCTA 
AAGACTCTGA GGAAGGACAG CTCGGGAAAA AAGGCTGCAG CCGCCGAAAA ACTAAGGGGC 
CGAGAGCTTT CAAGCAGCGG TTCTACTTTG GATGAGAAGT ATCTTCGGCA GCGTCGTGGG 
TCCCTCGACC GCTCACCAAC CAGAAAGCGC ACCTCGTCTC CAGATCGTCG ACGGGCGAAA 
TCCATGGACC GCAGGGCAGA GCGAGGACAT CGTGACCGCA CACCTGAGAG GGAACACGAT 
GATGGAGGGT TTCTCTCAGT CACCCCTGAA CGGAGGTTCC ACGAGCGAAG GCTCCTCAAG 
GACTCACAGA TAGCTGGACG AGTCAGTGAG GCTTCAACCC CCGAGCGCGC CAACAACAGC 
ATGGAATCCT TGTCTCGTTC CAGGGGCCGC ACGGACGATA AAGCCACAAA GAACGGCAAC 
CTTCTGAGAG ACTCTTCTTC AGAGAGGAGA GAGGACCGAC ATCGCATGAA TGGGACAATG 
GAGAGGCGAT ACAGAGCGGA GCGGGACTCT TCCCCTGACA GCAACTACAG CCGGGACGAG 
CTGAACTATG CAGAAGCACC TAGACTGAAG GACTACTACA GCATGATGAA GAACAACGCT 
GCACACAACA ATGCTACCTA CAACAACGCA GGCCATAACA ATAACACAGT AGGCCGCAGC 
TCAAACCAGG TCCCTGAGCC CAAGAGGAGG CTGTACAAAG AAAGTCCTAA AGACCTCAGC 
ATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108232031 ( XM_017409519.1 ) cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus) -> XP_017265008.1 Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X3, mRNA.

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CloneID	OKr06770
Clone ID Related Accession (Same CDS sequence)	XM_017409519.1 , XM_017409519.2
Accession Version	XM_017409519.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-27
Organism	Kryptolebias marmoratus(mangrove rivulus)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016094253.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Kryptolebias marmoratus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACGA AAGTGAAGGA GTGCGCCGTG 
GTTCGGGACC CCAGCGGGGA GCTGAACTTG GAGCTGCGCG GCGGGGCGCA GGACGGAGAG 
TTCGCCTACA TCGGACACAT CAGGGAGGAT GCTGTGCTCC ACCAGGACGG GAAACTGTCT 
GAGGGAGAGC TGCTGCTGGA GGTGGAGGGG CTGTCCGTGT CTGGATTACC TCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAAGGT CCCGTCAGGT TCAAAACTGT CCGCCAAGGT 
CACAAGCTCA ACAAGGACCT AAAGCACTTC CTGAGCTTGA GGTTTCAGAA GTCATCACCT 
GACCACGAGT TGCAGAAGAC TATCCGAGCC AATCTATATC GTCATGCCGT GCCTTGTACA 
ACCCGCGCCC CTCGAGACGG AGAGGTGCCA GGGGTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTTGAGGA GAGTGGCACA CTACTGGAAA TAGGAACGTA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC GCCGAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
ACGGGCTCCC ATCAGTCCAC CCCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAACGTC 
CGCGTAGTGC CTGCTGACGA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGAACCT TAGTGCGTAG CCTTTTCCCC TCTCCTTTGT TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGTAC CGGCATCCTT CGGCCATATC CTGTGCGTGA TAATGCTCTG 
GCCTGTGGTC TGAACAATGC CACCACCTCC ACTGTTGAAA GTGGGCAGCA GACGCTCGCA 
GAACCACAGG TCTCTCCAGA TGACCCTCTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACTGAGA ACGGGGAGCT GTACTTCATA GACACTGCAA AGCCTCACCA CCAGGACATG 
GCTGTGCATT TTCAGAGTGT CCACAACACA AAGACCACAT CATGGCTGGA TCCGCGGTGC 
CGAGACAAAG CCTCCAGGCC TTTGGAAGAG TGTGATGATG ATGAAGAAGG GATACATACG 
GAAGACCTGG AAAGTGATCT AGAGCTGCCC CCAGGATGGG AAAGAATAGA TGACCCCGTG 
TACGGAGTGT ACTATGTAGA TCATATAAAC AGGAAGACCC AATATGAGAA CCCAGTGGTG 
GAGGCGCGGC GCCAAAAACT CTTGGAGCAG CAGCAACAGC CGCAGCCTCC AGAAGAGTGG 
ATAGAGGAGC ACTCAGCTGC AGCAGCTCCT TTGGCAAACT ATGCTCCAAA CCACCTGGAG 
ACCTACAGGG ACCCACAAGT CCCTCCAGCT CTACCTCCAG GCCCCGCTGG CATCAAAAGA 
GGGAAACCTT TCTTTACGAG AAACCCAGCA GAGCTGAAGG GAACCTTCAT CAACACCAAG 
CTGAAGAAAA GTCACCGTGG ATTCGGGTTC ACCGTGGTGG GAGGTGACGA GCCCGACGAG 
TTCCTGCAGA TCAAGAGTCT GGTGCTGGAT GGGCCTGCGG CTGTTGATGG CAAGATGGAG 
ACAGGTGATG TGATTGTCAG CGTGAATGAC ACTATTGTGC TGGGCTACAC TCATGCTCAG 
GTGGTGAAGA TCTTCCAGTC CATCCCTATC GGCTCCATGG TGGACCTGGC CTTATGCCGT 
GGCTACCCAC TGCCCTTTGA CCCAGACGAC CCAAACACCA GCCTGGTGAC CTCTGTGGCC 
ATCTTGGACA AGGAACCCAT TATTGTCAAT GGGCAAGAGA CGTTTGACAC GCCGCCTAAC 
CAGGCTGGAC CCCTTAACAG CATGCGTGAG CCACGGCCTC TCAGCCCCTC AGCTGAGGTG 
GCGTCCAACG GCTCCCATGG TTACTCCAGC GACGTGGTCA CGTTGGCCTC GTCCATAGCC 
ACTCAGCCCG AGCTCATCAC CATCCACATG GAGAAGGGGG ACAAAGGTTT TGGCTTCACC 
ATCGCTGACA GCCTAACAGG CGGAGGCCAG AGGGTAAAGC AGATAGTGGA CTATCCACGG 
TGCCGCGGCC TGAAAGAGGG GGACATTCTA ATGGAGGTCA ACAAGAGAAA TGTCCAGAAT 
ATGAGTCACA ATCAGGTGGT GGATCTGCTG AGCAAATGTC CAAGAGGCAG TGAGGTCACC 
ATGTTGGTGC AAAGAGGAGT GGTGCCAGCC AAGAGGAGCC CAAAGCTGGT GCACCAACTG 
GAGAGAAAAG ACAGTCAGAA CAGCTCCCAG CACAGTGTGT GCAGCCATCG CAGCACCCAC 
ACCGATTCTC CCAGCCACCC ACCCTCTGCC ATGCCCAGTG AGGCAGTGGT CCCCACTCCC 
GCTGCCCCCA CTCAGCCTCT GCCAGTCTTG CCCCCCCAGG ACCCCGCAGA TGGCATGCTC 
ACCCTCCAGA AGAAGCCAGA CCCTTTTAAG ATCTGGGCAC AGTCCAGGAG CATGTATGAG 
AGTCGACTAC CAGATTGCCA GGAGCAAGAC ATTTTCCTTT GGCGGAAAGA TACAGGTTTT 
GGGTTTCGCA TCTTGGGAGG AAATGAGCCT GGGGAGCCTA TATACATAGG GCACATAGTG 
AAGTACGGGG CCGCAGATGA AGATGGCCGC CTGCGATCGG GTGACGAGCT CATCTGTGTG 
GACGGCACGG CTGTAGTGGG CAAATCTCAC CAGCTGGTGG TGCAGCTGAT GCAGCAGGCA 
GCCAAGCAGG GCCACGTCAA CCTCACTGTC AGACGCAAGA CCCCCGGATA CGGAGGGTCA 
AAAGGAGATG GTGACGTTCC TCCATCCCCA GCCTCCTCCC ACCACAGCAG CACCCAGGCA 
CCCAGTCTGA CGGAGGGAAA GCGCACCCCT CAGGGCAGCC AGAACTCACT CAACACTGTC 
AGCTCCGGCA GCGGATCCAC CAGCGGCATA GGCAGCGGCG GTGGAGGAGG CAGTGGGAGC 
GCGGTGGTGC CTGCCTCCCT GCAGCCTTAC GACGTGGAGA TCCAGCGAGG AGAGAACGAG 
GGATTTGGTT TTGTCATCGT GTCGTCCGTT TCCAGGCCTG ATGCTGGCAC TACTTTCGCT 
GGAAATGCTT GTGTGGCAAT GCCCCACAAA ATAGGACGCA TCATCGAGGG CAGCCCGGCG 
GACCGCTGCG GGAAGCTGAA GGTCGGGGAC AGAATACTGG CTGTTAACGG CTGCTCCATC 
ACCAACAAGT CTCACTCAGA CATCGTCAAC CTGATTAAGG AAGCCGGGAA CACCGTCTCA 
CTCAGGATCA TCCCAGGTGA TGAATCTTCC AATGCTTCTC TGCTGACTAA TGCTGAGAAG 
ATAGCTACCA TCACCACGAC ACACACACCT CATTCCACGG AGACCAGAAA TAATTCAAAG 
TCAAAAGGAG CTCCTCCTCC ACCCCCTTCA CAGACCCAAA CACAGGAAGC AGAGTTTTAT 
TCTGTGGACC TCGAGAGGGA CAGTAAAGGC TTCGGCTTCA GCTTAAGAGG AGGACGGGAG 
TACAACATGG ACCTGTACGT GTTGAGACTA GCAGAGGATG GGGCTGCTGT CAGAAATGGC 
AAGATGAGGG TGGGAGACGA GATCCTGGAG ATTAATGGTG AGAGCACAAA GAGCATGAAG 
CATTCACGAG CCATTGAACT GATCAAGAAC GGCGGTCGGA GGGCACGACT TGTCCTAAAA 
AGGGGCGATG GATCTGTACC TGAATATGAC GGCAGCAGTG ACGGGCACCC TCCCACACCC 
CGGGCACAAA ACACTCCGGA AGTGAGAACG TTGCCACCCA GCAGCCGATA TCACACCTCA 
TTGGAGTCCA GTTATCCACC AGACCTTCAC AAATCATCAC GCCTGGAGGA GGCTGCTTGT 
GGCCGAGGTA TGGACAAGAA CAGGGACAGG GCTGGTTCAG ATCAGATGGA ATTCCAAGGC 
CGACAATTTA ACCCCCATCA CCATTACACT TGGAATAATA ATCACAGTCA AAGTACCCCC 
AGACAGCAGT CCCCTGACAG TAATAGCACT AGCAGTGGCA ACAAGAAGAC CAGAGGCCGT 
AAAGTCAAGA AGTCTGAATC GGAGAGTGGC ATCTCTAAAC TTCTAAAGAC TCTGAGGAAG 
GACAGCTCGG GAAAAAAGGC TGCAGCCGCC GAAAAACTAA GGGGCCGAGA GCTTTCAAGC 
AGCGGTTCTA CTTTGGATGA GAAGTATCTT CGGCAGCGTC GTGGGTCCCT CGACCGCTCA 
CCAACCAGAA AGCGCACCTC GTCTCCAGAT CGTCGACGGG CGAAATCCAT GGACCGCAGG 
GCAGAGCGAG GACATCGTGA CCGCACACCT GAGAGGGAAC ACGATGATGG AGGGTTTCTC 
TCAGTCACCC CTGAACGGAG GTTCCACGAG CGAAGGCTCC TCAAGGACTC ACAGATAGCT 
GGACGAGTCA GTGAGGCTTC AACCCCCGAG CGCGCCAACA ACAGCATGGA ATCCTTGTCT 
CGTTCCAGGG GCCGCACGGA CGATAAAGCC ACAAAGAACG GCAACCTTCT GAGAGACTCT 
TCTTCAGAGA GGAGAGAGGA CCGACATCGC ATGAATGGGA CAATGGAGAG GCGATACAGA 
GCGGAGCGGG ACTCTTCCCC TGACAGCAAC TACAGCCGGG ACGAGCTGAA CTATGCAGAA 
GCACCTAGAC TGAAGGACTA CTACAGCATG ATGAAGAACA ACGCTGCACA CAACAATGCT 
ACCTACAACA ACGCAGGCCA TAACAATAAC ACAGTAGGCC GCAGCTCAAA CCAGGTCCCT 
GAGCCCAAGA GGAGGCTGTA CAAAGAAAGT CCTAAAGACC TCAGCATCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017265008.1
CDS	775..5625
Translation	MSKVIQKKNHWITKVKECAVVRDPSGELNLELRGGAQDGEFAYIGHIREDAVLHQDGKLSEGELLLEVEGLSVSGLPLYDIYTVINCCKGPVRFKTVRQGHKLNKDLKHFLSLRFQKSSPDHELQKTIRANLYRHAVPCTTRAPRDGEVPGVDYNFLSVEDFLQLEESGTLLEIGTYEGNYYGTPKPPRQPIIGTVTLSDTGSHQSTPKRTKSYNDMQNVRVVPADDDDDDQATEMNNSFTGTLVRSLFPSPLLCTGNPNDQDESRTGILRPYPVRDNALACGLNNATTSTVESGQQTLAEPQVSPDDPLGPLPENWEMAYTENGELYFIDTAKPHHQDMAVHFQSVHNTKTTSWLDPRCRDKASRPLEECDDDEEGIHTEDLESDLELPPGWERIDDPVYGVYYVDHINRKTQYENPVVEARRQKLLEQQQQPQPPEEWIEEHSAAAAPLANYAPNHLETYRDPQVPPALPPGPAGIKRGKPFFTRNPAELKGTFINTKLKKSHRGFGFTVVGGDEPDEFLQIKSLVLDGPAAVDGKMETGDVIVSVNDTIVLGYTHAQVVKIFQSIPIGSMVDLALCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETFDTPPNQAGPLNSMREPRPLSPSAEVASNGSHGYSSDVVTLASSIATQPELITIHMEKGDKGFGFTIADSLTGGGQRVKQIVDYPRCRGLKEGDILMEVNKRNVQNMSHNQVVDLLSKCPRGSEVTMLVQRGVVPAKRSPKLVHQLERKDSQNSSQHSVCSHRSTHTDSPSHPPSAMPSEAVVPTPAAPTQPLPVLPPQDPADGMLTLQKKPDPFKIWAQSRSMYESRLPDCQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTPGYGGSKGDGDVPPSPASSHHSSTQAPSLTEGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGSAVVPASLQPYDVEIQRGENEGFGFVIVSSVSRPDAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVSLRIIPGDESSNASLLTNAEKIATITTTHTPHSTETRNNSKSKGAPPPPPSQTQTQEAEFYSVDLERDSKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKSMKHSRAIELIKNGGRRARLVLKRGDGSVPEYDGSSDGHPPTPRAQNTPEVRTLPPSSRYHTSLESSYPPDLHKSSRLEEAACGRGMDKNRDRAGSDQMEFQGRQFNPHHHYTWNNNHSQSTPRQQSPDSNSTSSGNKKTRGRKVKKSESESGISKLLKTLRKDSSGKKAAAAEKLRGRELSSSGSTLDEKYLRQRRGSLDRSPTRKRTSSPDRRRAKSMDRRAERGHRDRTPEREHDDGGFLSVTPERRFHERRLLKDSQIAGRVSEASTPERANNSMESLSRSRGRTDDKATKNGNLLRDSSSERREDRHRMNGTMERRYRAERDSSPDSNYSRDELNYAEAPRLKDYYSMMKNNAAHNNATYNNAGHNNNTVGRSSNQVPEPKRRLYKESPKDLSI

Target ORF information:

RefSeq Version	XM_017409519.1
Organism	Kryptolebias marmoratus(mangrove rivulus)
Definition	Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017409519.1

ORF Insert Sequence:

ATGTCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACGA AAGTGAAGGA GTGCGCCGTG 
GTTCGGGACC CCAGCGGGGA GCTGAACTTG GAGCTGCGCG GCGGGGCGCA GGACGGAGAG 
TTCGCCTACA TCGGACACAT CAGGGAGGAT GCTGTGCTCC ACCAGGACGG GAAACTGTCT 
GAGGGAGAGC TGCTGCTGGA GGTGGAGGGG CTGTCCGTGT CTGGATTACC TCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAAGGT CCCGTCAGGT TCAAAACTGT CCGCCAAGGT 
CACAAGCTCA ACAAGGACCT AAAGCACTTC CTGAGCTTGA GGTTTCAGAA GTCATCACCT 
GACCACGAGT TGCAGAAGAC TATCCGAGCC AATCTATATC GTCATGCCGT GCCTTGTACA 
ACCCGCGCCC CTCGAGACGG AGAGGTGCCA GGGGTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTTGAGGA GAGTGGCACA CTACTGGAAA TAGGAACGTA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC GCCGAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
ACGGGCTCCC ATCAGTCCAC CCCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAACGTC 
CGCGTAGTGC CTGCTGACGA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGAACCT TAGTGCGTAG CCTTTTCCCC TCTCCTTTGT TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGTAC CGGCATCCTT CGGCCATATC CTGTGCGTGA TAATGCTCTG 
GCCTGTGGTC TGAACAATGC CACCACCTCC ACTGTTGAAA GTGGGCAGCA GACGCTCGCA 
GAACCACAGG TCTCTCCAGA TGACCCTCTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACTGAGA ACGGGGAGCT GTACTTCATA GACACTGCAA AGCCTCACCA CCAGGACATG 
GCTGTGCATT TTCAGAGTGT CCACAACACA AAGACCACAT CATGGCTGGA TCCGCGGTGC 
CGAGACAAAG CCTCCAGGCC TTTGGAAGAG TGTGATGATG ATGAAGAAGG GATACATACG 
GAAGACCTGG AAAGTGATCT AGAGCTGCCC CCAGGATGGG AAAGAATAGA TGACCCCGTG 
TACGGAGTGT ACTATGTAGA TCATATAAAC AGGAAGACCC AATATGAGAA CCCAGTGGTG 
GAGGCGCGGC GCCAAAAACT CTTGGAGCAG CAGCAACAGC CGCAGCCTCC AGAAGAGTGG 
ATAGAGGAGC ACTCAGCTGC AGCAGCTCCT TTGGCAAACT ATGCTCCAAA CCACCTGGAG 
ACCTACAGGG ACCCACAAGT CCCTCCAGCT CTACCTCCAG GCCCCGCTGG CATCAAAAGA 
GGGAAACCTT TCTTTACGAG AAACCCAGCA GAGCTGAAGG GAACCTTCAT CAACACCAAG 
CTGAAGAAAA GTCACCGTGG ATTCGGGTTC ACCGTGGTGG GAGGTGACGA GCCCGACGAG 
TTCCTGCAGA TCAAGAGTCT GGTGCTGGAT GGGCCTGCGG CTGTTGATGG CAAGATGGAG 
ACAGGTGATG TGATTGTCAG CGTGAATGAC ACTATTGTGC TGGGCTACAC TCATGCTCAG 
GTGGTGAAGA TCTTCCAGTC CATCCCTATC GGCTCCATGG TGGACCTGGC CTTATGCCGT 
GGCTACCCAC TGCCCTTTGA CCCAGACGAC CCAAACACCA GCCTGGTGAC CTCTGTGGCC 
ATCTTGGACA AGGAACCCAT TATTGTCAAT GGGCAAGAGA CGTTTGACAC GCCGCCTAAC 
CAGGCTGGAC CCCTTAACAG CATGCGTGAG CCACGGCCTC TCAGCCCCTC AGCTGAGGTG 
GCGTCCAACG GCTCCCATGG TTACTCCAGC GACGTGGTCA CGTTGGCCTC GTCCATAGCC 
ACTCAGCCCG AGCTCATCAC CATCCACATG GAGAAGGGGG ACAAAGGTTT TGGCTTCACC 
ATCGCTGACA GCCTAACAGG CGGAGGCCAG AGGGTAAAGC AGATAGTGGA CTATCCACGG 
TGCCGCGGCC TGAAAGAGGG GGACATTCTA ATGGAGGTCA ACAAGAGAAA TGTCCAGAAT 
ATGAGTCACA ATCAGGTGGT GGATCTGCTG AGCAAATGTC CAAGAGGCAG TGAGGTCACC 
ATGTTGGTGC AAAGAGGAGT GGTGCCAGCC AAGAGGAGCC CAAAGCTGGT GCACCAACTG 
GAGAGAAAAG ACAGTCAGAA CAGCTCCCAG CACAGTGTGT GCAGCCATCG CAGCACCCAC 
ACCGATTCTC CCAGCCACCC ACCCTCTGCC ATGCCCAGTG AGGCAGTGGT CCCCACTCCC 
GCTGCCCCCA CTCAGCCTCT GCCAGTCTTG CCCCCCCAGG ACCCCGCAGA TGGCATGCTC 
ACCCTCCAGA AGAAGCCAGA CCCTTTTAAG ATCTGGGCAC AGTCCAGGAG CATGTATGAG 
AGTCGACTAC CAGATTGCCA GGAGCAAGAC ATTTTCCTTT GGCGGAAAGA TACAGGTTTT 
GGGTTTCGCA TCTTGGGAGG AAATGAGCCT GGGGAGCCTA TATACATAGG GCACATAGTG 
AAGTACGGGG CCGCAGATGA AGATGGCCGC CTGCGATCGG GTGACGAGCT CATCTGTGTG 
GACGGCACGG CTGTAGTGGG CAAATCTCAC CAGCTGGTGG TGCAGCTGAT GCAGCAGGCA 
GCCAAGCAGG GCCACGTCAA CCTCACTGTC AGACGCAAGA CCCCCGGATA CGGAGGGTCA 
AAAGGAGATG GTGACGTTCC TCCATCCCCA GCCTCCTCCC ACCACAGCAG CACCCAGGCA 
CCCAGTCTGA CGGAGGGAAA GCGCACCCCT CAGGGCAGCC AGAACTCACT CAACACTGTC 
AGCTCCGGCA GCGGATCCAC CAGCGGCATA GGCAGCGGCG GTGGAGGAGG CAGTGGGAGC 
GCGGTGGTGC CTGCCTCCCT GCAGCCTTAC GACGTGGAGA TCCAGCGAGG AGAGAACGAG 
GGATTTGGTT TTGTCATCGT GTCGTCCGTT TCCAGGCCTG ATGCTGGCAC TACTTTCGCT 
GGAAATGCTT GTGTGGCAAT GCCCCACAAA ATAGGACGCA TCATCGAGGG CAGCCCGGCG 
GACCGCTGCG GGAAGCTGAA GGTCGGGGAC AGAATACTGG CTGTTAACGG CTGCTCCATC 
ACCAACAAGT CTCACTCAGA CATCGTCAAC CTGATTAAGG AAGCCGGGAA CACCGTCTCA 
CTCAGGATCA TCCCAGGTGA TGAATCTTCC AATGCTTCTC TGCTGACTAA TGCTGAGAAG 
ATAGCTACCA TCACCACGAC ACACACACCT CATTCCACGG AGACCAGAAA TAATTCAAAG 
TCAAAAGGAG CTCCTCCTCC ACCCCCTTCA CAGACCCAAA CACAGGAAGC AGAGTTTTAT 
TCTGTGGACC TCGAGAGGGA CAGTAAAGGC TTCGGCTTCA GCTTAAGAGG AGGACGGGAG 
TACAACATGG ACCTGTACGT GTTGAGACTA GCAGAGGATG GGGCTGCTGT CAGAAATGGC 
AAGATGAGGG TGGGAGACGA GATCCTGGAG ATTAATGGTG AGAGCACAAA GAGCATGAAG 
CATTCACGAG CCATTGAACT GATCAAGAAC GGCGGTCGGA GGGCACGACT TGTCCTAAAA 
AGGGGCGATG GATCTGTACC TGAATATGAC GGCAGCAGTG ACGGGCACCC TCCCACACCC 
CGGGCACAAA ACACTCCGGA AGTGAGAACG TTGCCACCCA GCAGCCGATA TCACACCTCA 
TTGGAGTCCA GTTATCCACC AGACCTTCAC AAATCATCAC GCCTGGAGGA GGCTGCTTGT 
GGCCGAGGTA TGGACAAGAA CAGGGACAGG GCTGGTTCAG ATCAGATGGA ATTCCAAGGC 
CGACAATTTA ACCCCCATCA CCATTACACT TGGAATAATA ATCACAGTCA AAGTACCCCC 
AGACAGCAGT CCCCTGACAG TAATAGCACT AGCAGTGGCA ACAAGAAGAC CAGAGGCCGT 
AAAGTCAAGA AGTCTGAATC GGAGAGTGGC ATCTCTAAAC TTCTAAAGAC TCTGAGGAAG 
GACAGCTCGG GAAAAAAGGC TGCAGCCGCC GAAAAACTAA GGGGCCGAGA GCTTTCAAGC 
AGCGGTTCTA CTTTGGATGA GAAGTATCTT CGGCAGCGTC GTGGGTCCCT CGACCGCTCA 
CCAACCAGAA AGCGCACCTC GTCTCCAGAT CGTCGACGGG CGAAATCCAT GGACCGCAGG 
GCAGAGCGAG GACATCGTGA CCGCACACCT GAGAGGGAAC ACGATGATGG AGGGTTTCTC 
TCAGTCACCC CTGAACGGAG GTTCCACGAG CGAAGGCTCC TCAAGGACTC ACAGATAGCT 
GGACGAGTCA GTGAGGCTTC AACCCCCGAG CGCGCCAACA ACAGCATGGA ATCCTTGTCT 
CGTTCCAGGG GCCGCACGGA CGATAAAGCC ACAAAGAACG GCAACCTTCT GAGAGACTCT 
TCTTCAGAGA GGAGAGAGGA CCGACATCGC ATGAATGGGA CAATGGAGAG GCGATACAGA 
GCGGAGCGGG ACTCTTCCCC TGACAGCAAC TACAGCCGGG ACGAGCTGAA CTATGCAGAA 
GCACCTAGAC TGAAGGACTA CTACAGCATG ATGAAGAACA ACGCTGCACA CAACAATGCT 
ACCTACAACA ACGCAGGCCA TAACAATAAC ACAGTAGGCC GCAGCTCAAA CCAGGTCCCT 
GAGCCCAAGA GGAGGCTGTA CAAAGAAAGT CCTAAAGACC TCAGCATCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108232031 ( XM_017409519.2 ) cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus) -> XP_017265008.1 Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X3, mRNA.

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CloneID	OKr06770
Clone ID Related Accession (Same CDS sequence)	XM_017409519.1 , XM_017409519.2
Accession Version	XM_017409519.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-03
Organism	Kryptolebias marmoratus(mangrove rivulus)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016094253.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 4, 2018 this sequence version replaced XM_017409519.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Kryptolebias marmoratus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACGA AAGTGAAGGA GTGCGCCGTG 
GTTCGGGACC CCAGCGGGGA GCTGAACTTG GAGCTGCGCG GCGGGGCGCA GGACGGAGAG 
TTCGCCTACA TCGGACACAT CAGGGAGGAT GCTGTGCTCC ACCAGGACGG GAAACTGTCT 
GAGGGAGAGC TGCTGCTGGA GGTGGAGGGG CTGTCCGTGT CTGGATTACC TCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAAGGT CCCGTCAGGT TCAAAACTGT CCGCCAAGGT 
CACAAGCTCA ACAAGGACCT AAAGCACTTC CTGAGCTTGA GGTTTCAGAA GTCATCACCT 
GACCACGAGT TGCAGAAGAC TATCCGAGCC AATCTATATC GTCATGCCGT GCCTTGTACA 
ACCCGCGCCC CTCGAGACGG AGAGGTGCCA GGGGTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTTGAGGA GAGTGGCACA CTACTGGAAA TAGGAACGTA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC GCCGAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
ACGGGCTCCC ATCAGTCCAC CCCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAACGTC 
CGCGTAGTGC CTGCTGACGA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGAACCT TAGTGCGTAG CCTTTTCCCC TCTCCTTTGT TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGTAC CGGCATCCTT CGGCCATATC CTGTGCGTGA TAATGCTCTG 
GCCTGTGGTC TGAACAATGC CACCACCTCC ACTGTTGAAA GTGGGCAGCA GACGCTCGCA 
GAACCACAGG TCTCTCCAGA TGACCCTCTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACTGAGA ACGGGGAGCT GTACTTCATA GACACTGCAA AGCCTCACCA CCAGGACATG 
GCTGTGCATT TTCAGAGTGT CCACAACACA AAGACCACAT CATGGCTGGA TCCGCGGTGC 
CGAGACAAAG CCTCCAGGCC TTTGGAAGAG TGTGATGATG ATGAAGAAGG GATACATACG 
GAAGACCTGG AAAGTGATCT AGAGCTGCCC CCAGGATGGG AAAGAATAGA TGACCCCGTG 
TACGGAGTGT ACTATGTAGA TCATATAAAC AGGAAGACCC AATATGAGAA CCCAGTGGTG 
GAGGCGCGGC GCCAAAAACT CTTGGAGCAG CAGCAACAGC CGCAGCCTCC AGAAGAGTGG 
ATAGAGGAGC ACTCAGCTGC AGCAGCTCCT TTGGCAAACT ATGCTCCAAA CCACCTGGAG 
ACCTACAGGG ACCCACAAGT CCCTCCAGCT CTACCTCCAG GCCCCGCTGG CATCAAAAGA 
GGGAAACCTT TCTTTACGAG AAACCCAGCA GAGCTGAAGG GAACCTTCAT CAACACCAAG 
CTGAAGAAAA GTCACCGTGG ATTCGGGTTC ACCGTGGTGG GAGGTGACGA GCCCGACGAG 
TTCCTGCAGA TCAAGAGTCT GGTGCTGGAT GGGCCTGCGG CTGTTGATGG CAAGATGGAG 
ACAGGTGATG TGATTGTCAG CGTGAATGAC ACTATTGTGC TGGGCTACAC TCATGCTCAG 
GTGGTGAAGA TCTTCCAGTC CATCCCTATC GGCTCCATGG TGGACCTGGC CTTATGCCGT 
GGCTACCCAC TGCCCTTTGA CCCAGACGAC CCAAACACCA GCCTGGTGAC CTCTGTGGCC 
ATCTTGGACA AGGAACCCAT TATTGTCAAT GGGCAAGAGA CGTTTGACAC GCCGCCTAAC 
CAGGCTGGAC CCCTTAACAG CATGCGTGAG CCACGGCCTC TCAGCCCCTC AGCTGAGGTG 
GCGTCCAACG GCTCCCATGG TTACTCCAGC GACGTGGTCA CGTTGGCCTC GTCCATAGCC 
ACTCAGCCCG AGCTCATCAC CATCCACATG GAGAAGGGGG ACAAAGGTTT TGGCTTCACC 
ATCGCTGACA GCCTAACAGG CGGAGGCCAG AGGGTAAAGC AGATAGTGGA CTATCCACGG 
TGCCGCGGCC TGAAAGAGGG GGACATTCTA ATGGAGGTCA ACAAGAGAAA TGTCCAGAAT 
ATGAGTCACA ATCAGGTGGT GGATCTGCTG AGCAAATGTC CAAGAGGCAG TGAGGTCACC 
ATGTTGGTGC AAAGAGGAGT GGTGCCAGCC AAGAGGAGCC CAAAGCTGGT GCACCAACTG 
GAGAGAAAAG ACAGTCAGAA CAGCTCCCAG CACAGTGTGT GCAGCCATCG CAGCACCCAC 
ACCGATTCTC CCAGCCACCC ACCCTCTGCC ATGCCCAGTG AGGCAGTGGT CCCCACTCCC 
GCTGCCCCCA CTCAGCCTCT GCCAGTCTTG CCCCCCCAGG ACCCCGCAGA TGGCATGCTC 
ACCCTCCAGA AGAAGCCAGA CCCTTTTAAG ATCTGGGCAC AGTCCAGGAG CATGTATGAG 
AGTCGACTAC CAGATTGCCA GGAGCAAGAC ATTTTCCTTT GGCGGAAAGA TACAGGTTTT 
GGGTTTCGCA TCTTGGGAGG AAATGAGCCT GGGGAGCCTA TATACATAGG GCACATAGTG 
AAGTACGGGG CCGCAGATGA AGATGGCCGC CTGCGATCGG GTGACGAGCT CATCTGTGTG 
GACGGCACGG CTGTAGTGGG CAAATCTCAC CAGCTGGTGG TGCAGCTGAT GCAGCAGGCA 
GCCAAGCAGG GCCACGTCAA CCTCACTGTC AGACGCAAGA CCCCCGGATA CGGAGGGTCA 
AAAGGAGATG GTGACGTTCC TCCATCCCCA GCCTCCTCCC ACCACAGCAG CACCCAGGCA 
CCCAGTCTGA CGGAGGGAAA GCGCACCCCT CAGGGCAGCC AGAACTCACT CAACACTGTC 
AGCTCCGGCA GCGGATCCAC CAGCGGCATA GGCAGCGGCG GTGGAGGAGG CAGTGGGAGC 
GCGGTGGTGC CTGCCTCCCT GCAGCCTTAC GACGTGGAGA TCCAGCGAGG AGAGAACGAG 
GGATTTGGTT TTGTCATCGT GTCGTCCGTT TCCAGGCCTG ATGCTGGCAC TACTTTCGCT 
GGAAATGCTT GTGTGGCAAT GCCCCACAAA ATAGGACGCA TCATCGAGGG CAGCCCGGCG 
GACCGCTGCG GGAAGCTGAA GGTCGGGGAC AGAATACTGG CTGTTAACGG CTGCTCCATC 
ACCAACAAGT CTCACTCAGA CATCGTCAAC CTGATTAAGG AAGCCGGGAA CACCGTCTCA 
CTCAGGATCA TCCCAGGTGA TGAATCTTCC AATGCTTCTC TGCTGACTAA TGCTGAGAAG 
ATAGCTACCA TCACCACGAC ACACACACCT CATTCCACGG AGACCAGAAA TAATTCAAAG 
TCAAAAGGAG CTCCTCCTCC ACCCCCTTCA CAGACCCAAA CACAGGAAGC AGAGTTTTAT 
TCTGTGGACC TCGAGAGGGA CAGTAAAGGC TTCGGCTTCA GCTTAAGAGG AGGACGGGAG 
TACAACATGG ACCTGTACGT GTTGAGACTA GCAGAGGATG GGGCTGCTGT CAGAAATGGC 
AAGATGAGGG TGGGAGACGA GATCCTGGAG ATTAATGGTG AGAGCACAAA GAGCATGAAG 
CATTCACGAG CCATTGAACT GATCAAGAAC GGCGGTCGGA GGGCACGACT TGTCCTAAAA 
AGGGGCGATG GATCTGTACC TGAATATGAC GGCAGCAGTG ACGGGCACCC TCCCACACCC 
CGGGCACAAA ACACTCCGGA AGTGAGAACG TTGCCACCCA GCAGCCGATA TCACACCTCA 
TTGGAGTCCA GTTATCCACC AGACCTTCAC AAATCATCAC GCCTGGAGGA GGCTGCTTGT 
GGCCGAGGTA TGGACAAGAA CAGGGACAGG GCTGGTTCAG ATCAGATGGA ATTCCAAGGC 
CGACAATTTA ACCCCCATCA CCATTACACT TGGAATAATA ATCACAGTCA AAGTACCCCC 
AGACAGCAGT CCCCTGACAG TAATAGCACT AGCAGTGGCA ACAAGAAGAC CAGAGGCCGT 
AAAGTCAAGA AGTCTGAATC GGAGAGTGGC ATCTCTAAAC TTCTAAAGAC TCTGAGGAAG 
GACAGCTCGG GAAAAAAGGC TGCAGCCGCC GAAAAACTAA GGGGCCGAGA GCTTTCAAGC 
AGCGGTTCTA CTTTGGATGA GAAGTATCTT CGGCAGCGTC GTGGGTCCCT CGACCGCTCA 
CCAACCAGAA AGCGCACCTC GTCTCCAGAT CGTCGACGGG CGAAATCCAT GGACCGCAGG 
GCAGAGCGAG GACATCGTGA CCGCACACCT GAGAGGGAAC ACGATGATGG AGGGTTTCTC 
TCAGTCACCC CTGAACGGAG GTTCCACGAG CGAAGGCTCC TCAAGGACTC ACAGATAGCT 
GGACGAGTCA GTGAGGCTTC AACCCCCGAG CGCGCCAACA ACAGCATGGA ATCCTTGTCT 
CGTTCCAGGG GCCGCACGGA CGATAAAGCC ACAAAGAACG GCAACCTTCT GAGAGACTCT 
TCTTCAGAGA GGAGAGAGGA CCGACATCGC ATGAATGGGA CAATGGAGAG GCGATACAGA 
GCGGAGCGGG ACTCTTCCCC TGACAGCAAC TACAGCCGGG ACGAGCTGAA CTATGCAGAA 
GCACCTAGAC TGAAGGACTA CTACAGCATG ATGAAGAACA ACGCTGCACA CAACAATGCT 
ACCTACAACA ACGCAGGCCA TAACAATAAC ACAGTAGGCC GCAGCTCAAA CCAGGTCCCT 
GAGCCCAAGA GGAGGCTGTA CAAAGAAAGT CCTAAAGACC TCAGCATCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017265008.1
CDS	893..5743
Translation	MSKVIQKKNHWITKVKECAVVRDPSGELNLELRGGAQDGEFAYIGHIREDAVLHQDGKLSEGELLLEVEGLSVSGLPLYDIYTVINCCKGPVRFKTVRQGHKLNKDLKHFLSLRFQKSSPDHELQKTIRANLYRHAVPCTTRAPRDGEVPGVDYNFLSVEDFLQLEESGTLLEIGTYEGNYYGTPKPPRQPIIGTVTLSDTGSHQSTPKRTKSYNDMQNVRVVPADDDDDDQATEMNNSFTGTLVRSLFPSPLLCTGNPNDQDESRTGILRPYPVRDNALACGLNNATTSTVESGQQTLAEPQVSPDDPLGPLPENWEMAYTENGELYFIDTAKPHHQDMAVHFQSVHNTKTTSWLDPRCRDKASRPLEECDDDEEGIHTEDLESDLELPPGWERIDDPVYGVYYVDHINRKTQYENPVVEARRQKLLEQQQQPQPPEEWIEEHSAAAAPLANYAPNHLETYRDPQVPPALPPGPAGIKRGKPFFTRNPAELKGTFINTKLKKSHRGFGFTVVGGDEPDEFLQIKSLVLDGPAAVDGKMETGDVIVSVNDTIVLGYTHAQVVKIFQSIPIGSMVDLALCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETFDTPPNQAGPLNSMREPRPLSPSAEVASNGSHGYSSDVVTLASSIATQPELITIHMEKGDKGFGFTIADSLTGGGQRVKQIVDYPRCRGLKEGDILMEVNKRNVQNMSHNQVVDLLSKCPRGSEVTMLVQRGVVPAKRSPKLVHQLERKDSQNSSQHSVCSHRSTHTDSPSHPPSAMPSEAVVPTPAAPTQPLPVLPPQDPADGMLTLQKKPDPFKIWAQSRSMYESRLPDCQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTPGYGGSKGDGDVPPSPASSHHSSTQAPSLTEGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGSAVVPASLQPYDVEIQRGENEGFGFVIVSSVSRPDAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVSLRIIPGDESSNASLLTNAEKIATITTTHTPHSTETRNNSKSKGAPPPPPSQTQTQEAEFYSVDLERDSKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKSMKHSRAIELIKNGGRRARLVLKRGDGSVPEYDGSSDGHPPTPRAQNTPEVRTLPPSSRYHTSLESSYPPDLHKSSRLEEAACGRGMDKNRDRAGSDQMEFQGRQFNPHHHYTWNNNHSQSTPRQQSPDSNSTSSGNKKTRGRKVKKSESESGISKLLKTLRKDSSGKKAAAAEKLRGRELSSSGSTLDEKYLRQRRGSLDRSPTRKRTSSPDRRRAKSMDRRAERGHRDRTPEREHDDGGFLSVTPERRFHERRLLKDSQIAGRVSEASTPERANNSMESLSRSRGRTDDKATKNGNLLRDSSSERREDRHRMNGTMERRYRAERDSSPDSNYSRDELNYAEAPRLKDYYSMMKNNAAHNNATYNNAGHNNNTVGRSSNQVPEPKRRLYKESPKDLSI

Target ORF information:

RefSeq Version	XM_017409519.2
Organism	Kryptolebias marmoratus(mangrove rivulus)
Definition	Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017409519.2

ORF Insert Sequence:

ATGTCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACGA AAGTGAAGGA GTGCGCCGTG 
GTTCGGGACC CCAGCGGGGA GCTGAACTTG GAGCTGCGCG GCGGGGCGCA GGACGGAGAG 
TTCGCCTACA TCGGACACAT CAGGGAGGAT GCTGTGCTCC ACCAGGACGG GAAACTGTCT 
GAGGGAGAGC TGCTGCTGGA GGTGGAGGGG CTGTCCGTGT CTGGATTACC TCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAAGGT CCCGTCAGGT TCAAAACTGT CCGCCAAGGT 
CACAAGCTCA ACAAGGACCT AAAGCACTTC CTGAGCTTGA GGTTTCAGAA GTCATCACCT 
GACCACGAGT TGCAGAAGAC TATCCGAGCC AATCTATATC GTCATGCCGT GCCTTGTACA 
ACCCGCGCCC CTCGAGACGG AGAGGTGCCA GGGGTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTTGAGGA GAGTGGCACA CTACTGGAAA TAGGAACGTA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC GCCGAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
ACGGGCTCCC ATCAGTCCAC CCCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAACGTC 
CGCGTAGTGC CTGCTGACGA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGAACCT TAGTGCGTAG CCTTTTCCCC TCTCCTTTGT TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGTAC CGGCATCCTT CGGCCATATC CTGTGCGTGA TAATGCTCTG 
GCCTGTGGTC TGAACAATGC CACCACCTCC ACTGTTGAAA GTGGGCAGCA GACGCTCGCA 
GAACCACAGG TCTCTCCAGA TGACCCTCTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACTGAGA ACGGGGAGCT GTACTTCATA GACACTGCAA AGCCTCACCA CCAGGACATG 
GCTGTGCATT TTCAGAGTGT CCACAACACA AAGACCACAT CATGGCTGGA TCCGCGGTGC 
CGAGACAAAG CCTCCAGGCC TTTGGAAGAG TGTGATGATG ATGAAGAAGG GATACATACG 
GAAGACCTGG AAAGTGATCT AGAGCTGCCC CCAGGATGGG AAAGAATAGA TGACCCCGTG 
TACGGAGTGT ACTATGTAGA TCATATAAAC AGGAAGACCC AATATGAGAA CCCAGTGGTG 
GAGGCGCGGC GCCAAAAACT CTTGGAGCAG CAGCAACAGC CGCAGCCTCC AGAAGAGTGG 
ATAGAGGAGC ACTCAGCTGC AGCAGCTCCT TTGGCAAACT ATGCTCCAAA CCACCTGGAG 
ACCTACAGGG ACCCACAAGT CCCTCCAGCT CTACCTCCAG GCCCCGCTGG CATCAAAAGA 
GGGAAACCTT TCTTTACGAG AAACCCAGCA GAGCTGAAGG GAACCTTCAT CAACACCAAG 
CTGAAGAAAA GTCACCGTGG ATTCGGGTTC ACCGTGGTGG GAGGTGACGA GCCCGACGAG 
TTCCTGCAGA TCAAGAGTCT GGTGCTGGAT GGGCCTGCGG CTGTTGATGG CAAGATGGAG 
ACAGGTGATG TGATTGTCAG CGTGAATGAC ACTATTGTGC TGGGCTACAC TCATGCTCAG 
GTGGTGAAGA TCTTCCAGTC CATCCCTATC GGCTCCATGG TGGACCTGGC CTTATGCCGT 
GGCTACCCAC TGCCCTTTGA CCCAGACGAC CCAAACACCA GCCTGGTGAC CTCTGTGGCC 
ATCTTGGACA AGGAACCCAT TATTGTCAAT GGGCAAGAGA CGTTTGACAC GCCGCCTAAC 
CAGGCTGGAC CCCTTAACAG CATGCGTGAG CCACGGCCTC TCAGCCCCTC AGCTGAGGTG 
GCGTCCAACG GCTCCCATGG TTACTCCAGC GACGTGGTCA CGTTGGCCTC GTCCATAGCC 
ACTCAGCCCG AGCTCATCAC CATCCACATG GAGAAGGGGG ACAAAGGTTT TGGCTTCACC 
ATCGCTGACA GCCTAACAGG CGGAGGCCAG AGGGTAAAGC AGATAGTGGA CTATCCACGG 
TGCCGCGGCC TGAAAGAGGG GGACATTCTA ATGGAGGTCA ACAAGAGAAA TGTCCAGAAT 
ATGAGTCACA ATCAGGTGGT GGATCTGCTG AGCAAATGTC CAAGAGGCAG TGAGGTCACC 
ATGTTGGTGC AAAGAGGAGT GGTGCCAGCC AAGAGGAGCC CAAAGCTGGT GCACCAACTG 
GAGAGAAAAG ACAGTCAGAA CAGCTCCCAG CACAGTGTGT GCAGCCATCG CAGCACCCAC 
ACCGATTCTC CCAGCCACCC ACCCTCTGCC ATGCCCAGTG AGGCAGTGGT CCCCACTCCC 
GCTGCCCCCA CTCAGCCTCT GCCAGTCTTG CCCCCCCAGG ACCCCGCAGA TGGCATGCTC 
ACCCTCCAGA AGAAGCCAGA CCCTTTTAAG ATCTGGGCAC AGTCCAGGAG CATGTATGAG 
AGTCGACTAC CAGATTGCCA GGAGCAAGAC ATTTTCCTTT GGCGGAAAGA TACAGGTTTT 
GGGTTTCGCA TCTTGGGAGG AAATGAGCCT GGGGAGCCTA TATACATAGG GCACATAGTG 
AAGTACGGGG CCGCAGATGA AGATGGCCGC CTGCGATCGG GTGACGAGCT CATCTGTGTG 
GACGGCACGG CTGTAGTGGG CAAATCTCAC CAGCTGGTGG TGCAGCTGAT GCAGCAGGCA 
GCCAAGCAGG GCCACGTCAA CCTCACTGTC AGACGCAAGA CCCCCGGATA CGGAGGGTCA 
AAAGGAGATG GTGACGTTCC TCCATCCCCA GCCTCCTCCC ACCACAGCAG CACCCAGGCA 
CCCAGTCTGA CGGAGGGAAA GCGCACCCCT CAGGGCAGCC AGAACTCACT CAACACTGTC 
AGCTCCGGCA GCGGATCCAC CAGCGGCATA GGCAGCGGCG GTGGAGGAGG CAGTGGGAGC 
GCGGTGGTGC CTGCCTCCCT GCAGCCTTAC GACGTGGAGA TCCAGCGAGG AGAGAACGAG 
GGATTTGGTT TTGTCATCGT GTCGTCCGTT TCCAGGCCTG ATGCTGGCAC TACTTTCGCT 
GGAAATGCTT GTGTGGCAAT GCCCCACAAA ATAGGACGCA TCATCGAGGG CAGCCCGGCG 
GACCGCTGCG GGAAGCTGAA GGTCGGGGAC AGAATACTGG CTGTTAACGG CTGCTCCATC 
ACCAACAAGT CTCACTCAGA CATCGTCAAC CTGATTAAGG AAGCCGGGAA CACCGTCTCA 
CTCAGGATCA TCCCAGGTGA TGAATCTTCC AATGCTTCTC TGCTGACTAA TGCTGAGAAG 
ATAGCTACCA TCACCACGAC ACACACACCT CATTCCACGG AGACCAGAAA TAATTCAAAG 
TCAAAAGGAG CTCCTCCTCC ACCCCCTTCA CAGACCCAAA CACAGGAAGC AGAGTTTTAT 
TCTGTGGACC TCGAGAGGGA CAGTAAAGGC TTCGGCTTCA GCTTAAGAGG AGGACGGGAG 
TACAACATGG ACCTGTACGT GTTGAGACTA GCAGAGGATG GGGCTGCTGT CAGAAATGGC 
AAGATGAGGG TGGGAGACGA GATCCTGGAG ATTAATGGTG AGAGCACAAA GAGCATGAAG 
CATTCACGAG CCATTGAACT GATCAAGAAC GGCGGTCGGA GGGCACGACT TGTCCTAAAA 
AGGGGCGATG GATCTGTACC TGAATATGAC GGCAGCAGTG ACGGGCACCC TCCCACACCC 
CGGGCACAAA ACACTCCGGA AGTGAGAACG TTGCCACCCA GCAGCCGATA TCACACCTCA 
TTGGAGTCCA GTTATCCACC AGACCTTCAC AAATCATCAC GCCTGGAGGA GGCTGCTTGT 
GGCCGAGGTA TGGACAAGAA CAGGGACAGG GCTGGTTCAG ATCAGATGGA ATTCCAAGGC 
CGACAATTTA ACCCCCATCA CCATTACACT TGGAATAATA ATCACAGTCA AAGTACCCCC 
AGACAGCAGT CCCCTGACAG TAATAGCACT AGCAGTGGCA ACAAGAAGAC CAGAGGCCGT 
AAAGTCAAGA AGTCTGAATC GGAGAGTGGC ATCTCTAAAC TTCTAAAGAC TCTGAGGAAG 
GACAGCTCGG GAAAAAAGGC TGCAGCCGCC GAAAAACTAA GGGGCCGAGA GCTTTCAAGC 
AGCGGTTCTA CTTTGGATGA GAAGTATCTT CGGCAGCGTC GTGGGTCCCT CGACCGCTCA 
CCAACCAGAA AGCGCACCTC GTCTCCAGAT CGTCGACGGG CGAAATCCAT GGACCGCAGG 
GCAGAGCGAG GACATCGTGA CCGCACACCT GAGAGGGAAC ACGATGATGG AGGGTTTCTC 
TCAGTCACCC CTGAACGGAG GTTCCACGAG CGAAGGCTCC TCAAGGACTC ACAGATAGCT 
GGACGAGTCA GTGAGGCTTC AACCCCCGAG CGCGCCAACA ACAGCATGGA ATCCTTGTCT 
CGTTCCAGGG GCCGCACGGA CGATAAAGCC ACAAAGAACG GCAACCTTCT GAGAGACTCT 
TCTTCAGAGA GGAGAGAGGA CCGACATCGC ATGAATGGGA CAATGGAGAG GCGATACAGA 
GCGGAGCGGG ACTCTTCCCC TGACAGCAAC TACAGCCGGG ACGAGCTGAA CTATGCAGAA 
GCACCTAGAC TGAAGGACTA CTACAGCATG ATGAAGAACA ACGCTGCACA CAACAATGCT 
ACCTACAACA ACGCAGGCCA TAACAATAAC ACAGTAGGCC GCAGCTCAAA CCAGGTCCCT 
GAGCCCAAGA GGAGGCTGTA CAAAGAAAGT CCTAAAGACC TCAGCATCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108232031 ( XM_017409520.1 ) cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus) -> XP_017265009.1 Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OKr06771
Clone ID Related Accession (Same CDS sequence)	XM_017409520.1 , XM_017409520.2
Accession Version	XM_017409520.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-27
Organism	Kryptolebias marmoratus(mangrove rivulus)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016094253.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Kryptolebias marmoratus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACGA AAGTGAAGGA GTGCGCCGTG 
GTTCGGGACC CCAGCGGGGA GCTGAACTTG GAGCTGCGCG GCGGGGCGCA GGACGGAGAG 
TTCGCCTACA TCGGACACAT CAGGGAGGAT GCTGTGCTCC ACCAGGACGG GAAACTGTCT 
GAGGGAGAGC TGCTGCTGGA GGTGGAGGGG CTGTCCGTGT CTGGATTACC TCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAAGGT CCCGTCAGGT TCAAAACTGT CCGCCAAGGT 
CACAAGCTCA ACAAGGACCT AAAGCACTTC CTGAGCTTGA GGTTTCAGAA GTCATCACCT 
GACCACGAGT TGCAGAAGAC TATCCGAGCC AATCTATATC GTCATGCCGT GCCTTGTACA 
ACCCGCGCCC CTCGAGACGG AGAGGTGCCA GGGGTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTTGAGGA GAGTGGCACA CTACTGGAAA TAGGAACGTA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC GCCGAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
ACGGGCTCCC ATCAGTCCAC CCCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAACGTC 
CGCGTAGTGC CTGCTGACGA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGTAACC CTAACGACCA GGACGAGAGC CGTACCGGCA TCCTTCGGCC ATATCCTGTG 
CGTGATAATG CTCTGGCCTG TGGTCTGAAC AATGCCACCA CCTCCACTGT TGAAAGTGGG 
CAGCAGACGC TCGCAGAACC ACAGGTCTCT CCAGATGACC CTCTGGGACC GCTGCCTGAA 
AACTGGGAGA TGGCCTACAC TGAGAACGGG GAGCTGTACT TCATAGACAC TGCAAAGCCT 
CACCACCAGG ACATGGCTGT GCATTTTCAG AGTGTCCACA ACACAAAGAC CACATCATGG 
CTGGATCCGC GGTGCCGAGA CAAAGCCTCC AGGCCTTTGG AAGAGTGTGA TGATGATGAA 
GAAGGGATAC ATACGGAAGA CCTGGAAAGT GATCTAGAGC TGCCCCCAGG ATGGGAAAGA 
ATAGATGACC CCGTGTACGG AGTGTACTAT GTAGATCATA TAAACAGGAA GACCCAATAT 
GAGAACCCAG TGGTGGAGGC GCGGCGCCAA AAACTCTTGG AGCAGCAGCA ACAGCCGCAG 
CCTCCAGAAG GTGAGCGGTA TATTCGAGAG TGGATAGAGG AGCACTCAGC TGCAGCAGCT 
CCTTTGGCAA ACTATGCTCC AAACCACCTG GAGACCTACA GGGACCCACA AGTCCCTCCA 
GCTCTACCTC CAGGCCCCGC TGGCATCAAA AGAGGGAAAC CTTTCTTTAC GAGAAACCCA 
GCAGAGCTGA AGGGAACCTT CATCAACACC AAGCTGAAGA AAAGTCACCG TGGATTCGGG 
TTCACCGTGG TGGGAGGTGA CGAGCCCGAC GAGTTCCTGC AGATCAAGAG TCTGGTGCTG 
GATGGGCCTG CGGCTGTTGA TGGCAAGATG GAGACAGGTG ATGTGATTGT CAGCGTGAAT 
GACACTATTG TGCTGGGCTA CACTCATGCT CAGGTGGTGA AGATCTTCCA GTCCATCCCT 
ATCGGCTCCA TGGTGGACCT GGCCTTATGC CGTGGCTACC CACTGCCCTT TGACCCAGAC 
GACCCAAACA CCAGCCTGGT GACCTCTGTG GCCATCTTGG ACAAGGAACC CATTATTGTC 
AATGGGCAAG AGACGTTTGA CACGCCGCCT AACCAGGCTG GACCCCTTAA CAGCATGCGT 
GAGCCACGGC CTCTCAGCCC CTCAGCTGAG GTGGCGTCCA ACGGCTCCCA TGGTTACTCC 
AGCGACGTGG TCACGTTGGC CTCGTCCATA GCCACTCAGC CCGAGCTCAT CACCATCCAC 
ATGGAGAAGG GGGACAAAGG TTTTGGCTTC ACCATCGCTG ACAGCCTAAC AGGCGGAGGC 
CAGAGGGTAA AGCAGATAGT GGACTATCCA CGGTGCCGCG GCCTGAAAGA GGGGGACATT 
CTAATGGAGG TCAACAAGAG AAATGTCCAG AATATGAGTC ACAATCAGGT GGTGGATCTG 
CTGAGCAAAT GTCCAAGAGG CAGTGAGGTC ACCATGTTGG TGCAAAGAGG AGTGGTGCCA 
GCCAAGAGGA GCCCAAAGCT GGTGCACCAA CTGGAGAGAA AAGACAGTCA GAACAGCTCC 
CAGCACAGTG TGTGCAGCCA TCGCAGCACC CACACCGATT CTCCCAGCCA CCCACCCTCT 
GCCATGCCCA GTGAGGCAGT GGTCCCCACT CCCGCTGCCC CCACTCAGCC TCTGCCAGTC 
TTGCCCCCCC AGGACCCCGC AGATGGCATG CTCACCCTCC AGAAGAAGCC AGACCCTTTT 
AAGATCTGGG CACAGTCCAG GAGCATGTAT GAGAGTCGAC TACCAGATTG CCAGGAGCAA 
GACATTTTCC TTTGGCGGAA AGATACAGGT TTTGGGTTTC GCATCTTGGG AGGAAATGAG 
CCTGGGGAGC CTATATACAT AGGGCACATA GTGAAGTACG GGGCCGCAGA TGAAGATGGC 
CGCCTGCGAT CGGGTGACGA GCTCATCTGT GTGGACGGCA CGGCTGTAGT GGGCAAATCT 
CACCAGCTGG TGGTGCAGCT GATGCAGCAG GCAGCCAAGC AGGGCCACGT CAACCTCACT 
GTCAGACGCA AGACCCCCGG ATACGGAGGG TCAAAAGGAG ATGGTGACGT TCCTCCATCC 
CCAGCCTCCT CCCACCACAG CAGCACCCAG GCACCCAGTC TGACGGAGGG AAAGCGCACC 
CCTCAGGGCA GCCAGAACTC ACTCAACACT GTCAGCTCCG GCAGCGGATC CACCAGCGGC 
ATAGGCAGCG GCGGTGGAGG AGGCAGTGGG AGCGCGGTGG TGCCTGCCTC CCTGCAGCCT 
TACGACGTGG AGATCCAGCG AGGAGAGAAC GAGGGATTTG GTTTTGTCAT CGTGTCGTCC 
GTTTCCAGGC CTGATGCTGG CACTACTTTC GCTGGAAATG CTTGTGTGGC AATGCCCCAC 
AAAATAGGAC GCATCATCGA GGGCAGCCCG GCGGACCGCT GCGGGAAGCT GAAGGTCGGG 
GACAGAATAC TGGCTGTTAA CGGCTGCTCC ATCACCAACA AGTCTCACTC AGACATCGTC 
AACCTGATTA AGGAAGCCGG GAACACCGTC TCACTCAGGA TCATCCCAGG TGATGAATCT 
TCCAATGCTT CTCTGCTGAC TAATGCTGAG AAGATAGCTA CCATCACCAC GACACACACA 
CCTCATTCCA CGGAGACCAG AAATAATTCA AAGTCAAAAG GAGCTCCTCC TCCACCCCCT 
TCACAGACCC AAACACAGGA AGCAGAGTTT TATTCTGTGG ACCTCGAGAG GGACAGTAAA 
GGCTTCGGCT TCAGCTTAAG AGGAGGACGG GAGTACAACA TGGACCTGTA CGTGTTGAGA 
CTAGCAGAGG ATGGGGCTGC TGTCAGAAAT GGCAAGATGA GGGTGGGAGA CGAGATCCTG 
GAGATTAATG GTGAGAGCAC AAAGAGCATG AAGCATTCAC GAGCCATTGA ACTGATCAAG 
AACGGCGGTC GGAGGGCACG ACTTGTCCTA AAAAGGGGCG ATGGATCTGT ACCTGAATAT 
GACGGCAGCA GTGACGGGCA CCCTCCCACA CCCCGGGCAC AAAACACTCC GGAAGTGAGA 
ACGTTGCCAC CCAGCAGCCG ATATCACACC TCATTGGAGT CCAGTTATCC ACCAGACCTT 
CACAAATCAT CACGCCTGGA GGAGGCTGCT TGTGGCCGAG GTATGGACAA GAACAGGGAC 
AGGGCTGGTT CAGATCAGAT GGAATTCCAA GGCCGACAAT TTAACCCCCA TCACCATTAC 
ACTTGGAATA ATAATCACAG TCAAAGTACC CCCAGACAGC AGTCCCCTGA CAGTAATAGC 
ACTAGCAGTG GCAACAAGAA GACCAGAGGC CGTAAAGTCA AGAAGTCTGA ATCGGAGAGT 
GGCATCTCTA AACTTCTAAA GACTCTGAGG AAGGACAGCT CGGGAAAAAA GGCTGCAGCC 
GCCGAAAAAC TAAGGGGCCG AGAGCTTTCA AGCAGCGGTT CTACTTTGGA TGAGAAGTAT 
CTTCGGCAGC GTCGTGGGTC CCTCGACCGC TCACCAACCA GAAAGCGCAC CTCGTCTCCA 
GATCGTCGAC GGGCGAAATC CATGGACCGC AGGGCAGAGC GAGGACATCG TGACCGCACA 
CCTGAGAGGG AACACGATGA TGGAGGGTTT CTCTCAGTCA CCCCTGAACG GAGGTTCCAC 
GAGCGAAGGC TCCTCAAGGA CTCACAGATA GCTGGACGAG TCAGTGAGGC TTCAACCCCC 
GAGCGCGCCA ACAACAGCAT GGAATCCTTG TCTCGTTCCA GGGGCCGCAC GGACGATAAA 
GCCACAAAGA ACGGCAACCT TCTGAGAGAC TCTTCTTCAG AGAGGAGAGA GGACCGACAT 
CGCATGAATG GGACAATGGA GAGGCGATAC AGAGCGGAGC GGGACTCTTC CCCTGACAGC 
AACTACAGCC GGGACGAGCT GAACTATGCA GAAGCACCTA GACTGAAGGA CTACTACAGC 
ATGATGAAGA ACAACGCTGC ACACAACAAT GCTACCTACA ACAACGCAGG CCATAACAAT 
AACACAGTAG GCCGCAGCTC AAACCAGGTC CCTGAGCCCA AGAGGAGGCT GTACAAAGAA 
AGTCCTAAAG ACCTCAGCAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017265009.1
CDS	774..5597
Translation	MSKVIQKKNHWITKVKECAVVRDPSGELNLELRGGAQDGEFAYIGHIREDAVLHQDGKLSEGELLLEVEGLSVSGLPLYDIYTVINCCKGPVRFKTVRQGHKLNKDLKHFLSLRFQKSSPDHELQKTIRANLYRHAVPCTTRAPRDGEVPGVDYNFLSVEDFLQLEESGTLLEIGTYEGNYYGTPKPPRQPIIGTVTLSDTGSHQSTPKRTKSYNDMQNVRVVPADDDDDDQATEMNNSFTGNPNDQDESRTGILRPYPVRDNALACGLNNATTSTVESGQQTLAEPQVSPDDPLGPLPENWEMAYTENGELYFIDTAKPHHQDMAVHFQSVHNTKTTSWLDPRCRDKASRPLEECDDDEEGIHTEDLESDLELPPGWERIDDPVYGVYYVDHINRKTQYENPVVEARRQKLLEQQQQPQPPEGERYIREWIEEHSAAAAPLANYAPNHLETYRDPQVPPALPPGPAGIKRGKPFFTRNPAELKGTFINTKLKKSHRGFGFTVVGGDEPDEFLQIKSLVLDGPAAVDGKMETGDVIVSVNDTIVLGYTHAQVVKIFQSIPIGSMVDLALCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETFDTPPNQAGPLNSMREPRPLSPSAEVASNGSHGYSSDVVTLASSIATQPELITIHMEKGDKGFGFTIADSLTGGGQRVKQIVDYPRCRGLKEGDILMEVNKRNVQNMSHNQVVDLLSKCPRGSEVTMLVQRGVVPAKRSPKLVHQLERKDSQNSSQHSVCSHRSTHTDSPSHPPSAMPSEAVVPTPAAPTQPLPVLPPQDPADGMLTLQKKPDPFKIWAQSRSMYESRLPDCQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTPGYGGSKGDGDVPPSPASSHHSSTQAPSLTEGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGSAVVPASLQPYDVEIQRGENEGFGFVIVSSVSRPDAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVSLRIIPGDESSNASLLTNAEKIATITTTHTPHSTETRNNSKSKGAPPPPPSQTQTQEAEFYSVDLERDSKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKSMKHSRAIELIKNGGRRARLVLKRGDGSVPEYDGSSDGHPPTPRAQNTPEVRTLPPSSRYHTSLESSYPPDLHKSSRLEEAACGRGMDKNRDRAGSDQMEFQGRQFNPHHHYTWNNNHSQSTPRQQSPDSNSTSSGNKKTRGRKVKKSESESGISKLLKTLRKDSSGKKAAAAEKLRGRELSSSGSTLDEKYLRQRRGSLDRSPTRKRTSSPDRRRAKSMDRRAERGHRDRTPEREHDDGGFLSVTPERRFHERRLLKDSQIAGRVSEASTPERANNSMESLSRSRGRTDDKATKNGNLLRDSSSERREDRHRMNGTMERRYRAERDSSPDSNYSRDELNYAEAPRLKDYYSMMKNNAAHNNATYNNAGHNNNTVGRSSNQVPEPKRRLYKESPKDLSI

Target ORF information:

RefSeq Version	XM_017409520.1
Organism	Kryptolebias marmoratus(mangrove rivulus)
Definition	Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017409520.1

ORF Insert Sequence:

ATGTCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACGA AAGTGAAGGA GTGCGCCGTG 
GTTCGGGACC CCAGCGGGGA GCTGAACTTG GAGCTGCGCG GCGGGGCGCA GGACGGAGAG 
TTCGCCTACA TCGGACACAT CAGGGAGGAT GCTGTGCTCC ACCAGGACGG GAAACTGTCT 
GAGGGAGAGC TGCTGCTGGA GGTGGAGGGG CTGTCCGTGT CTGGATTACC TCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAAGGT CCCGTCAGGT TCAAAACTGT CCGCCAAGGT 
CACAAGCTCA ACAAGGACCT AAAGCACTTC CTGAGCTTGA GGTTTCAGAA GTCATCACCT 
GACCACGAGT TGCAGAAGAC TATCCGAGCC AATCTATATC GTCATGCCGT GCCTTGTACA 
ACCCGCGCCC CTCGAGACGG AGAGGTGCCA GGGGTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTTGAGGA GAGTGGCACA CTACTGGAAA TAGGAACGTA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC GCCGAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
ACGGGCTCCC ATCAGTCCAC CCCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAACGTC 
CGCGTAGTGC CTGCTGACGA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGTAACC CTAACGACCA GGACGAGAGC CGTACCGGCA TCCTTCGGCC ATATCCTGTG 
CGTGATAATG CTCTGGCCTG TGGTCTGAAC AATGCCACCA CCTCCACTGT TGAAAGTGGG 
CAGCAGACGC TCGCAGAACC ACAGGTCTCT CCAGATGACC CTCTGGGACC GCTGCCTGAA 
AACTGGGAGA TGGCCTACAC TGAGAACGGG GAGCTGTACT TCATAGACAC TGCAAAGCCT 
CACCACCAGG ACATGGCTGT GCATTTTCAG AGTGTCCACA ACACAAAGAC CACATCATGG 
CTGGATCCGC GGTGCCGAGA CAAAGCCTCC AGGCCTTTGG AAGAGTGTGA TGATGATGAA 
GAAGGGATAC ATACGGAAGA CCTGGAAAGT GATCTAGAGC TGCCCCCAGG ATGGGAAAGA 
ATAGATGACC CCGTGTACGG AGTGTACTAT GTAGATCATA TAAACAGGAA GACCCAATAT 
GAGAACCCAG TGGTGGAGGC GCGGCGCCAA AAACTCTTGG AGCAGCAGCA ACAGCCGCAG 
CCTCCAGAAG GTGAGCGGTA TATTCGAGAG TGGATAGAGG AGCACTCAGC TGCAGCAGCT 
CCTTTGGCAA ACTATGCTCC AAACCACCTG GAGACCTACA GGGACCCACA AGTCCCTCCA 
GCTCTACCTC CAGGCCCCGC TGGCATCAAA AGAGGGAAAC CTTTCTTTAC GAGAAACCCA 
GCAGAGCTGA AGGGAACCTT CATCAACACC AAGCTGAAGA AAAGTCACCG TGGATTCGGG 
TTCACCGTGG TGGGAGGTGA CGAGCCCGAC GAGTTCCTGC AGATCAAGAG TCTGGTGCTG 
GATGGGCCTG CGGCTGTTGA TGGCAAGATG GAGACAGGTG ATGTGATTGT CAGCGTGAAT 
GACACTATTG TGCTGGGCTA CACTCATGCT CAGGTGGTGA AGATCTTCCA GTCCATCCCT 
ATCGGCTCCA TGGTGGACCT GGCCTTATGC CGTGGCTACC CACTGCCCTT TGACCCAGAC 
GACCCAAACA CCAGCCTGGT GACCTCTGTG GCCATCTTGG ACAAGGAACC CATTATTGTC 
AATGGGCAAG AGACGTTTGA CACGCCGCCT AACCAGGCTG GACCCCTTAA CAGCATGCGT 
GAGCCACGGC CTCTCAGCCC CTCAGCTGAG GTGGCGTCCA ACGGCTCCCA TGGTTACTCC 
AGCGACGTGG TCACGTTGGC CTCGTCCATA GCCACTCAGC CCGAGCTCAT CACCATCCAC 
ATGGAGAAGG GGGACAAAGG TTTTGGCTTC ACCATCGCTG ACAGCCTAAC AGGCGGAGGC 
CAGAGGGTAA AGCAGATAGT GGACTATCCA CGGTGCCGCG GCCTGAAAGA GGGGGACATT 
CTAATGGAGG TCAACAAGAG AAATGTCCAG AATATGAGTC ACAATCAGGT GGTGGATCTG 
CTGAGCAAAT GTCCAAGAGG CAGTGAGGTC ACCATGTTGG TGCAAAGAGG AGTGGTGCCA 
GCCAAGAGGA GCCCAAAGCT GGTGCACCAA CTGGAGAGAA AAGACAGTCA GAACAGCTCC 
CAGCACAGTG TGTGCAGCCA TCGCAGCACC CACACCGATT CTCCCAGCCA CCCACCCTCT 
GCCATGCCCA GTGAGGCAGT GGTCCCCACT CCCGCTGCCC CCACTCAGCC TCTGCCAGTC 
TTGCCCCCCC AGGACCCCGC AGATGGCATG CTCACCCTCC AGAAGAAGCC AGACCCTTTT 
AAGATCTGGG CACAGTCCAG GAGCATGTAT GAGAGTCGAC TACCAGATTG CCAGGAGCAA 
GACATTTTCC TTTGGCGGAA AGATACAGGT TTTGGGTTTC GCATCTTGGG AGGAAATGAG 
CCTGGGGAGC CTATATACAT AGGGCACATA GTGAAGTACG GGGCCGCAGA TGAAGATGGC 
CGCCTGCGAT CGGGTGACGA GCTCATCTGT GTGGACGGCA CGGCTGTAGT GGGCAAATCT 
CACCAGCTGG TGGTGCAGCT GATGCAGCAG GCAGCCAAGC AGGGCCACGT CAACCTCACT 
GTCAGACGCA AGACCCCCGG ATACGGAGGG TCAAAAGGAG ATGGTGACGT TCCTCCATCC 
CCAGCCTCCT CCCACCACAG CAGCACCCAG GCACCCAGTC TGACGGAGGG AAAGCGCACC 
CCTCAGGGCA GCCAGAACTC ACTCAACACT GTCAGCTCCG GCAGCGGATC CACCAGCGGC 
ATAGGCAGCG GCGGTGGAGG AGGCAGTGGG AGCGCGGTGG TGCCTGCCTC CCTGCAGCCT 
TACGACGTGG AGATCCAGCG AGGAGAGAAC GAGGGATTTG GTTTTGTCAT CGTGTCGTCC 
GTTTCCAGGC CTGATGCTGG CACTACTTTC GCTGGAAATG CTTGTGTGGC AATGCCCCAC 
AAAATAGGAC GCATCATCGA GGGCAGCCCG GCGGACCGCT GCGGGAAGCT GAAGGTCGGG 
GACAGAATAC TGGCTGTTAA CGGCTGCTCC ATCACCAACA AGTCTCACTC AGACATCGTC 
AACCTGATTA AGGAAGCCGG GAACACCGTC TCACTCAGGA TCATCCCAGG TGATGAATCT 
TCCAATGCTT CTCTGCTGAC TAATGCTGAG AAGATAGCTA CCATCACCAC GACACACACA 
CCTCATTCCA CGGAGACCAG AAATAATTCA AAGTCAAAAG GAGCTCCTCC TCCACCCCCT 
TCACAGACCC AAACACAGGA AGCAGAGTTT TATTCTGTGG ACCTCGAGAG GGACAGTAAA 
GGCTTCGGCT TCAGCTTAAG AGGAGGACGG GAGTACAACA TGGACCTGTA CGTGTTGAGA 
CTAGCAGAGG ATGGGGCTGC TGTCAGAAAT GGCAAGATGA GGGTGGGAGA CGAGATCCTG 
GAGATTAATG GTGAGAGCAC AAAGAGCATG AAGCATTCAC GAGCCATTGA ACTGATCAAG 
AACGGCGGTC GGAGGGCACG ACTTGTCCTA AAAAGGGGCG ATGGATCTGT ACCTGAATAT 
GACGGCAGCA GTGACGGGCA CCCTCCCACA CCCCGGGCAC AAAACACTCC GGAAGTGAGA 
ACGTTGCCAC CCAGCAGCCG ATATCACACC TCATTGGAGT CCAGTTATCC ACCAGACCTT 
CACAAATCAT CACGCCTGGA GGAGGCTGCT TGTGGCCGAG GTATGGACAA GAACAGGGAC 
AGGGCTGGTT CAGATCAGAT GGAATTCCAA GGCCGACAAT TTAACCCCCA TCACCATTAC 
ACTTGGAATA ATAATCACAG TCAAAGTACC CCCAGACAGC AGTCCCCTGA CAGTAATAGC 
ACTAGCAGTG GCAACAAGAA GACCAGAGGC CGTAAAGTCA AGAAGTCTGA ATCGGAGAGT 
GGCATCTCTA AACTTCTAAA GACTCTGAGG AAGGACAGCT CGGGAAAAAA GGCTGCAGCC 
GCCGAAAAAC TAAGGGGCCG AGAGCTTTCA AGCAGCGGTT CTACTTTGGA TGAGAAGTAT 
CTTCGGCAGC GTCGTGGGTC CCTCGACCGC TCACCAACCA GAAAGCGCAC CTCGTCTCCA 
GATCGTCGAC GGGCGAAATC CATGGACCGC AGGGCAGAGC GAGGACATCG TGACCGCACA 
CCTGAGAGGG AACACGATGA TGGAGGGTTT CTCTCAGTCA CCCCTGAACG GAGGTTCCAC 
GAGCGAAGGC TCCTCAAGGA CTCACAGATA GCTGGACGAG TCAGTGAGGC TTCAACCCCC 
GAGCGCGCCA ACAACAGCAT GGAATCCTTG TCTCGTTCCA GGGGCCGCAC GGACGATAAA 
GCCACAAAGA ACGGCAACCT TCTGAGAGAC TCTTCTTCAG AGAGGAGAGA GGACCGACAT 
CGCATGAATG GGACAATGGA GAGGCGATAC AGAGCGGAGC GGGACTCTTC CCCTGACAGC 
AACTACAGCC GGGACGAGCT GAACTATGCA GAAGCACCTA GACTGAAGGA CTACTACAGC 
ATGATGAAGA ACAACGCTGC ACACAACAAT GCTACCTACA ACAACGCAGG CCATAACAAT 
AACACAGTAG GCCGCAGCTC AAACCAGGTC CCTGAGCCCA AGAGGAGGCT GTACAAAGAA 
AGTCCTAAAG ACCTCAGCAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108232031 ( XM_017409520.2 ) cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus) -> XP_017265009.1 Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X5, mRNA.

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CloneID	OKr06771
Clone ID Related Accession (Same CDS sequence)	XM_017409520.1 , XM_017409520.2
Accession Version	XM_017409520.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-03
Organism	Kryptolebias marmoratus(mangrove rivulus)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016094253.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 4, 2018 this sequence version replaced XM_017409520.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Kryptolebias marmoratus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACGA AAGTGAAGGA GTGCGCCGTG 
GTTCGGGACC CCAGCGGGGA GCTGAACTTG GAGCTGCGCG GCGGGGCGCA GGACGGAGAG 
TTCGCCTACA TCGGACACAT CAGGGAGGAT GCTGTGCTCC ACCAGGACGG GAAACTGTCT 
GAGGGAGAGC TGCTGCTGGA GGTGGAGGGG CTGTCCGTGT CTGGATTACC TCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAAGGT CCCGTCAGGT TCAAAACTGT CCGCCAAGGT 
CACAAGCTCA ACAAGGACCT AAAGCACTTC CTGAGCTTGA GGTTTCAGAA GTCATCACCT 
GACCACGAGT TGCAGAAGAC TATCCGAGCC AATCTATATC GTCATGCCGT GCCTTGTACA 
ACCCGCGCCC CTCGAGACGG AGAGGTGCCA GGGGTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTTGAGGA GAGTGGCACA CTACTGGAAA TAGGAACGTA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC GCCGAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
ACGGGCTCCC ATCAGTCCAC CCCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAACGTC 
CGCGTAGTGC CTGCTGACGA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGTAACC CTAACGACCA GGACGAGAGC CGTACCGGCA TCCTTCGGCC ATATCCTGTG 
CGTGATAATG CTCTGGCCTG TGGTCTGAAC AATGCCACCA CCTCCACTGT TGAAAGTGGG 
CAGCAGACGC TCGCAGAACC ACAGGTCTCT CCAGATGACC CTCTGGGACC GCTGCCTGAA 
AACTGGGAGA TGGCCTACAC TGAGAACGGG GAGCTGTACT TCATAGACAC TGCAAAGCCT 
CACCACCAGG ACATGGCTGT GCATTTTCAG AGTGTCCACA ACACAAAGAC CACATCATGG 
CTGGATCCGC GGTGCCGAGA CAAAGCCTCC AGGCCTTTGG AAGAGTGTGA TGATGATGAA 
GAAGGGATAC ATACGGAAGA CCTGGAAAGT GATCTAGAGC TGCCCCCAGG ATGGGAAAGA 
ATAGATGACC CCGTGTACGG AGTGTACTAT GTAGATCATA TAAACAGGAA GACCCAATAT 
GAGAACCCAG TGGTGGAGGC GCGGCGCCAA AAACTCTTGG AGCAGCAGCA ACAGCCGCAG 
CCTCCAGAAG GTGAGCGGTA TATTCGAGAG TGGATAGAGG AGCACTCAGC TGCAGCAGCT 
CCTTTGGCAA ACTATGCTCC AAACCACCTG GAGACCTACA GGGACCCACA AGTCCCTCCA 
GCTCTACCTC CAGGCCCCGC TGGCATCAAA AGAGGGAAAC CTTTCTTTAC GAGAAACCCA 
GCAGAGCTGA AGGGAACCTT CATCAACACC AAGCTGAAGA AAAGTCACCG TGGATTCGGG 
TTCACCGTGG TGGGAGGTGA CGAGCCCGAC GAGTTCCTGC AGATCAAGAG TCTGGTGCTG 
GATGGGCCTG CGGCTGTTGA TGGCAAGATG GAGACAGGTG ATGTGATTGT CAGCGTGAAT 
GACACTATTG TGCTGGGCTA CACTCATGCT CAGGTGGTGA AGATCTTCCA GTCCATCCCT 
ATCGGCTCCA TGGTGGACCT GGCCTTATGC CGTGGCTACC CACTGCCCTT TGACCCAGAC 
GACCCAAACA CCAGCCTGGT GACCTCTGTG GCCATCTTGG ACAAGGAACC CATTATTGTC 
AATGGGCAAG AGACGTTTGA CACGCCGCCT AACCAGGCTG GACCCCTTAA CAGCATGCGT 
GAGCCACGGC CTCTCAGCCC CTCAGCTGAG GTGGCGTCCA ACGGCTCCCA TGGTTACTCC 
AGCGACGTGG TCACGTTGGC CTCGTCCATA GCCACTCAGC CCGAGCTCAT CACCATCCAC 
ATGGAGAAGG GGGACAAAGG TTTTGGCTTC ACCATCGCTG ACAGCCTAAC AGGCGGAGGC 
CAGAGGGTAA AGCAGATAGT GGACTATCCA CGGTGCCGCG GCCTGAAAGA GGGGGACATT 
CTAATGGAGG TCAACAAGAG AAATGTCCAG AATATGAGTC ACAATCAGGT GGTGGATCTG 
CTGAGCAAAT GTCCAAGAGG CAGTGAGGTC ACCATGTTGG TGCAAAGAGG AGTGGTGCCA 
GCCAAGAGGA GCCCAAAGCT GGTGCACCAA CTGGAGAGAA AAGACAGTCA GAACAGCTCC 
CAGCACAGTG TGTGCAGCCA TCGCAGCACC CACACCGATT CTCCCAGCCA CCCACCCTCT 
GCCATGCCCA GTGAGGCAGT GGTCCCCACT CCCGCTGCCC CCACTCAGCC TCTGCCAGTC 
TTGCCCCCCC AGGACCCCGC AGATGGCATG CTCACCCTCC AGAAGAAGCC AGACCCTTTT 
AAGATCTGGG CACAGTCCAG GAGCATGTAT GAGAGTCGAC TACCAGATTG CCAGGAGCAA 
GACATTTTCC TTTGGCGGAA AGATACAGGT TTTGGGTTTC GCATCTTGGG AGGAAATGAG 
CCTGGGGAGC CTATATACAT AGGGCACATA GTGAAGTACG GGGCCGCAGA TGAAGATGGC 
CGCCTGCGAT CGGGTGACGA GCTCATCTGT GTGGACGGCA CGGCTGTAGT GGGCAAATCT 
CACCAGCTGG TGGTGCAGCT GATGCAGCAG GCAGCCAAGC AGGGCCACGT CAACCTCACT 
GTCAGACGCA AGACCCCCGG ATACGGAGGG TCAAAAGGAG ATGGTGACGT TCCTCCATCC 
CCAGCCTCCT CCCACCACAG CAGCACCCAG GCACCCAGTC TGACGGAGGG AAAGCGCACC 
CCTCAGGGCA GCCAGAACTC ACTCAACACT GTCAGCTCCG GCAGCGGATC CACCAGCGGC 
ATAGGCAGCG GCGGTGGAGG AGGCAGTGGG AGCGCGGTGG TGCCTGCCTC CCTGCAGCCT 
TACGACGTGG AGATCCAGCG AGGAGAGAAC GAGGGATTTG GTTTTGTCAT CGTGTCGTCC 
GTTTCCAGGC CTGATGCTGG CACTACTTTC GCTGGAAATG CTTGTGTGGC AATGCCCCAC 
AAAATAGGAC GCATCATCGA GGGCAGCCCG GCGGACCGCT GCGGGAAGCT GAAGGTCGGG 
GACAGAATAC TGGCTGTTAA CGGCTGCTCC ATCACCAACA AGTCTCACTC AGACATCGTC 
AACCTGATTA AGGAAGCCGG GAACACCGTC TCACTCAGGA TCATCCCAGG TGATGAATCT 
TCCAATGCTT CTCTGCTGAC TAATGCTGAG AAGATAGCTA CCATCACCAC GACACACACA 
CCTCATTCCA CGGAGACCAG AAATAATTCA AAGTCAAAAG GAGCTCCTCC TCCACCCCCT 
TCACAGACCC AAACACAGGA AGCAGAGTTT TATTCTGTGG ACCTCGAGAG GGACAGTAAA 
GGCTTCGGCT TCAGCTTAAG AGGAGGACGG GAGTACAACA TGGACCTGTA CGTGTTGAGA 
CTAGCAGAGG ATGGGGCTGC TGTCAGAAAT GGCAAGATGA GGGTGGGAGA CGAGATCCTG 
GAGATTAATG GTGAGAGCAC AAAGAGCATG AAGCATTCAC GAGCCATTGA ACTGATCAAG 
AACGGCGGTC GGAGGGCACG ACTTGTCCTA AAAAGGGGCG ATGGATCTGT ACCTGAATAT 
GACGGCAGCA GTGACGGGCA CCCTCCCACA CCCCGGGCAC AAAACACTCC GGAAGTGAGA 
ACGTTGCCAC CCAGCAGCCG ATATCACACC TCATTGGAGT CCAGTTATCC ACCAGACCTT 
CACAAATCAT CACGCCTGGA GGAGGCTGCT TGTGGCCGAG GTATGGACAA GAACAGGGAC 
AGGGCTGGTT CAGATCAGAT GGAATTCCAA GGCCGACAAT TTAACCCCCA TCACCATTAC 
ACTTGGAATA ATAATCACAG TCAAAGTACC CCCAGACAGC AGTCCCCTGA CAGTAATAGC 
ACTAGCAGTG GCAACAAGAA GACCAGAGGC CGTAAAGTCA AGAAGTCTGA ATCGGAGAGT 
GGCATCTCTA AACTTCTAAA GACTCTGAGG AAGGACAGCT CGGGAAAAAA GGCTGCAGCC 
GCCGAAAAAC TAAGGGGCCG AGAGCTTTCA AGCAGCGGTT CTACTTTGGA TGAGAAGTAT 
CTTCGGCAGC GTCGTGGGTC CCTCGACCGC TCACCAACCA GAAAGCGCAC CTCGTCTCCA 
GATCGTCGAC GGGCGAAATC CATGGACCGC AGGGCAGAGC GAGGACATCG TGACCGCACA 
CCTGAGAGGG AACACGATGA TGGAGGGTTT CTCTCAGTCA CCCCTGAACG GAGGTTCCAC 
GAGCGAAGGC TCCTCAAGGA CTCACAGATA GCTGGACGAG TCAGTGAGGC TTCAACCCCC 
GAGCGCGCCA ACAACAGCAT GGAATCCTTG TCTCGTTCCA GGGGCCGCAC GGACGATAAA 
GCCACAAAGA ACGGCAACCT TCTGAGAGAC TCTTCTTCAG AGAGGAGAGA GGACCGACAT 
CGCATGAATG GGACAATGGA GAGGCGATAC AGAGCGGAGC GGGACTCTTC CCCTGACAGC 
AACTACAGCC GGGACGAGCT GAACTATGCA GAAGCACCTA GACTGAAGGA CTACTACAGC 
ATGATGAAGA ACAACGCTGC ACACAACAAT GCTACCTACA ACAACGCAGG CCATAACAAT 
AACACAGTAG GCCGCAGCTC AAACCAGGTC CCTGAGCCCA AGAGGAGGCT GTACAAAGAA 
AGTCCTAAAG ACCTCAGCAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017265009.1
CDS	891..5714
Translation	MSKVIQKKNHWITKVKECAVVRDPSGELNLELRGGAQDGEFAYIGHIREDAVLHQDGKLSEGELLLEVEGLSVSGLPLYDIYTVINCCKGPVRFKTVRQGHKLNKDLKHFLSLRFQKSSPDHELQKTIRANLYRHAVPCTTRAPRDGEVPGVDYNFLSVEDFLQLEESGTLLEIGTYEGNYYGTPKPPRQPIIGTVTLSDTGSHQSTPKRTKSYNDMQNVRVVPADDDDDDQATEMNNSFTGNPNDQDESRTGILRPYPVRDNALACGLNNATTSTVESGQQTLAEPQVSPDDPLGPLPENWEMAYTENGELYFIDTAKPHHQDMAVHFQSVHNTKTTSWLDPRCRDKASRPLEECDDDEEGIHTEDLESDLELPPGWERIDDPVYGVYYVDHINRKTQYENPVVEARRQKLLEQQQQPQPPEGERYIREWIEEHSAAAAPLANYAPNHLETYRDPQVPPALPPGPAGIKRGKPFFTRNPAELKGTFINTKLKKSHRGFGFTVVGGDEPDEFLQIKSLVLDGPAAVDGKMETGDVIVSVNDTIVLGYTHAQVVKIFQSIPIGSMVDLALCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETFDTPPNQAGPLNSMREPRPLSPSAEVASNGSHGYSSDVVTLASSIATQPELITIHMEKGDKGFGFTIADSLTGGGQRVKQIVDYPRCRGLKEGDILMEVNKRNVQNMSHNQVVDLLSKCPRGSEVTMLVQRGVVPAKRSPKLVHQLERKDSQNSSQHSVCSHRSTHTDSPSHPPSAMPSEAVVPTPAAPTQPLPVLPPQDPADGMLTLQKKPDPFKIWAQSRSMYESRLPDCQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTPGYGGSKGDGDVPPSPASSHHSSTQAPSLTEGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGSAVVPASLQPYDVEIQRGENEGFGFVIVSSVSRPDAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVSLRIIPGDESSNASLLTNAEKIATITTTHTPHSTETRNNSKSKGAPPPPPSQTQTQEAEFYSVDLERDSKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKSMKHSRAIELIKNGGRRARLVLKRGDGSVPEYDGSSDGHPPTPRAQNTPEVRTLPPSSRYHTSLESSYPPDLHKSSRLEEAACGRGMDKNRDRAGSDQMEFQGRQFNPHHHYTWNNNHSQSTPRQQSPDSNSTSSGNKKTRGRKVKKSESESGISKLLKTLRKDSSGKKAAAAEKLRGRELSSSGSTLDEKYLRQRRGSLDRSPTRKRTSSPDRRRAKSMDRRAERGHRDRTPEREHDDGGFLSVTPERRFHERRLLKDSQIAGRVSEASTPERANNSMESLSRSRGRTDDKATKNGNLLRDSSSERREDRHRMNGTMERRYRAERDSSPDSNYSRDELNYAEAPRLKDYYSMMKNNAAHNNATYNNAGHNNNTVGRSSNQVPEPKRRLYKESPKDLSI

Target ORF information:

RefSeq Version	XM_017409520.2
Organism	Kryptolebias marmoratus(mangrove rivulus)
Definition	Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017409520.2

ORF Insert Sequence:

ATGTCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACGA AAGTGAAGGA GTGCGCCGTG 
GTTCGGGACC CCAGCGGGGA GCTGAACTTG GAGCTGCGCG GCGGGGCGCA GGACGGAGAG 
TTCGCCTACA TCGGACACAT CAGGGAGGAT GCTGTGCTCC ACCAGGACGG GAAACTGTCT 
GAGGGAGAGC TGCTGCTGGA GGTGGAGGGG CTGTCCGTGT CTGGATTACC TCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAAGGT CCCGTCAGGT TCAAAACTGT CCGCCAAGGT 
CACAAGCTCA ACAAGGACCT AAAGCACTTC CTGAGCTTGA GGTTTCAGAA GTCATCACCT 
GACCACGAGT TGCAGAAGAC TATCCGAGCC AATCTATATC GTCATGCCGT GCCTTGTACA 
ACCCGCGCCC CTCGAGACGG AGAGGTGCCA GGGGTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTTGAGGA GAGTGGCACA CTACTGGAAA TAGGAACGTA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC GCCGAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
ACGGGCTCCC ATCAGTCCAC CCCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAACGTC 
CGCGTAGTGC CTGCTGACGA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGTAACC CTAACGACCA GGACGAGAGC CGTACCGGCA TCCTTCGGCC ATATCCTGTG 
CGTGATAATG CTCTGGCCTG TGGTCTGAAC AATGCCACCA CCTCCACTGT TGAAAGTGGG 
CAGCAGACGC TCGCAGAACC ACAGGTCTCT CCAGATGACC CTCTGGGACC GCTGCCTGAA 
AACTGGGAGA TGGCCTACAC TGAGAACGGG GAGCTGTACT TCATAGACAC TGCAAAGCCT 
CACCACCAGG ACATGGCTGT GCATTTTCAG AGTGTCCACA ACACAAAGAC CACATCATGG 
CTGGATCCGC GGTGCCGAGA CAAAGCCTCC AGGCCTTTGG AAGAGTGTGA TGATGATGAA 
GAAGGGATAC ATACGGAAGA CCTGGAAAGT GATCTAGAGC TGCCCCCAGG ATGGGAAAGA 
ATAGATGACC CCGTGTACGG AGTGTACTAT GTAGATCATA TAAACAGGAA GACCCAATAT 
GAGAACCCAG TGGTGGAGGC GCGGCGCCAA AAACTCTTGG AGCAGCAGCA ACAGCCGCAG 
CCTCCAGAAG GTGAGCGGTA TATTCGAGAG TGGATAGAGG AGCACTCAGC TGCAGCAGCT 
CCTTTGGCAA ACTATGCTCC AAACCACCTG GAGACCTACA GGGACCCACA AGTCCCTCCA 
GCTCTACCTC CAGGCCCCGC TGGCATCAAA AGAGGGAAAC CTTTCTTTAC GAGAAACCCA 
GCAGAGCTGA AGGGAACCTT CATCAACACC AAGCTGAAGA AAAGTCACCG TGGATTCGGG 
TTCACCGTGG TGGGAGGTGA CGAGCCCGAC GAGTTCCTGC AGATCAAGAG TCTGGTGCTG 
GATGGGCCTG CGGCTGTTGA TGGCAAGATG GAGACAGGTG ATGTGATTGT CAGCGTGAAT 
GACACTATTG TGCTGGGCTA CACTCATGCT CAGGTGGTGA AGATCTTCCA GTCCATCCCT 
ATCGGCTCCA TGGTGGACCT GGCCTTATGC CGTGGCTACC CACTGCCCTT TGACCCAGAC 
GACCCAAACA CCAGCCTGGT GACCTCTGTG GCCATCTTGG ACAAGGAACC CATTATTGTC 
AATGGGCAAG AGACGTTTGA CACGCCGCCT AACCAGGCTG GACCCCTTAA CAGCATGCGT 
GAGCCACGGC CTCTCAGCCC CTCAGCTGAG GTGGCGTCCA ACGGCTCCCA TGGTTACTCC 
AGCGACGTGG TCACGTTGGC CTCGTCCATA GCCACTCAGC CCGAGCTCAT CACCATCCAC 
ATGGAGAAGG GGGACAAAGG TTTTGGCTTC ACCATCGCTG ACAGCCTAAC AGGCGGAGGC 
CAGAGGGTAA AGCAGATAGT GGACTATCCA CGGTGCCGCG GCCTGAAAGA GGGGGACATT 
CTAATGGAGG TCAACAAGAG AAATGTCCAG AATATGAGTC ACAATCAGGT GGTGGATCTG 
CTGAGCAAAT GTCCAAGAGG CAGTGAGGTC ACCATGTTGG TGCAAAGAGG AGTGGTGCCA 
GCCAAGAGGA GCCCAAAGCT GGTGCACCAA CTGGAGAGAA AAGACAGTCA GAACAGCTCC 
CAGCACAGTG TGTGCAGCCA TCGCAGCACC CACACCGATT CTCCCAGCCA CCCACCCTCT 
GCCATGCCCA GTGAGGCAGT GGTCCCCACT CCCGCTGCCC CCACTCAGCC TCTGCCAGTC 
TTGCCCCCCC AGGACCCCGC AGATGGCATG CTCACCCTCC AGAAGAAGCC AGACCCTTTT 
AAGATCTGGG CACAGTCCAG GAGCATGTAT GAGAGTCGAC TACCAGATTG CCAGGAGCAA 
GACATTTTCC TTTGGCGGAA AGATACAGGT TTTGGGTTTC GCATCTTGGG AGGAAATGAG 
CCTGGGGAGC CTATATACAT AGGGCACATA GTGAAGTACG GGGCCGCAGA TGAAGATGGC 
CGCCTGCGAT CGGGTGACGA GCTCATCTGT GTGGACGGCA CGGCTGTAGT GGGCAAATCT 
CACCAGCTGG TGGTGCAGCT GATGCAGCAG GCAGCCAAGC AGGGCCACGT CAACCTCACT 
GTCAGACGCA AGACCCCCGG ATACGGAGGG TCAAAAGGAG ATGGTGACGT TCCTCCATCC 
CCAGCCTCCT CCCACCACAG CAGCACCCAG GCACCCAGTC TGACGGAGGG AAAGCGCACC 
CCTCAGGGCA GCCAGAACTC ACTCAACACT GTCAGCTCCG GCAGCGGATC CACCAGCGGC 
ATAGGCAGCG GCGGTGGAGG AGGCAGTGGG AGCGCGGTGG TGCCTGCCTC CCTGCAGCCT 
TACGACGTGG AGATCCAGCG AGGAGAGAAC GAGGGATTTG GTTTTGTCAT CGTGTCGTCC 
GTTTCCAGGC CTGATGCTGG CACTACTTTC GCTGGAAATG CTTGTGTGGC AATGCCCCAC 
AAAATAGGAC GCATCATCGA GGGCAGCCCG GCGGACCGCT GCGGGAAGCT GAAGGTCGGG 
GACAGAATAC TGGCTGTTAA CGGCTGCTCC ATCACCAACA AGTCTCACTC AGACATCGTC 
AACCTGATTA AGGAAGCCGG GAACACCGTC TCACTCAGGA TCATCCCAGG TGATGAATCT 
TCCAATGCTT CTCTGCTGAC TAATGCTGAG AAGATAGCTA CCATCACCAC GACACACACA 
CCTCATTCCA CGGAGACCAG AAATAATTCA AAGTCAAAAG GAGCTCCTCC TCCACCCCCT 
TCACAGACCC AAACACAGGA AGCAGAGTTT TATTCTGTGG ACCTCGAGAG GGACAGTAAA 
GGCTTCGGCT TCAGCTTAAG AGGAGGACGG GAGTACAACA TGGACCTGTA CGTGTTGAGA 
CTAGCAGAGG ATGGGGCTGC TGTCAGAAAT GGCAAGATGA GGGTGGGAGA CGAGATCCTG 
GAGATTAATG GTGAGAGCAC AAAGAGCATG AAGCATTCAC GAGCCATTGA ACTGATCAAG 
AACGGCGGTC GGAGGGCACG ACTTGTCCTA AAAAGGGGCG ATGGATCTGT ACCTGAATAT 
GACGGCAGCA GTGACGGGCA CCCTCCCACA CCCCGGGCAC AAAACACTCC GGAAGTGAGA 
ACGTTGCCAC CCAGCAGCCG ATATCACACC TCATTGGAGT CCAGTTATCC ACCAGACCTT 
CACAAATCAT CACGCCTGGA GGAGGCTGCT TGTGGCCGAG GTATGGACAA GAACAGGGAC 
AGGGCTGGTT CAGATCAGAT GGAATTCCAA GGCCGACAAT TTAACCCCCA TCACCATTAC 
ACTTGGAATA ATAATCACAG TCAAAGTACC CCCAGACAGC AGTCCCCTGA CAGTAATAGC 
ACTAGCAGTG GCAACAAGAA GACCAGAGGC CGTAAAGTCA AGAAGTCTGA ATCGGAGAGT 
GGCATCTCTA AACTTCTAAA GACTCTGAGG AAGGACAGCT CGGGAAAAAA GGCTGCAGCC 
GCCGAAAAAC TAAGGGGCCG AGAGCTTTCA AGCAGCGGTT CTACTTTGGA TGAGAAGTAT 
CTTCGGCAGC GTCGTGGGTC CCTCGACCGC TCACCAACCA GAAAGCGCAC CTCGTCTCCA 
GATCGTCGAC GGGCGAAATC CATGGACCGC AGGGCAGAGC GAGGACATCG TGACCGCACA 
CCTGAGAGGG AACACGATGA TGGAGGGTTT CTCTCAGTCA CCCCTGAACG GAGGTTCCAC 
GAGCGAAGGC TCCTCAAGGA CTCACAGATA GCTGGACGAG TCAGTGAGGC TTCAACCCCC 
GAGCGCGCCA ACAACAGCAT GGAATCCTTG TCTCGTTCCA GGGGCCGCAC GGACGATAAA 
GCCACAAAGA ACGGCAACCT TCTGAGAGAC TCTTCTTCAG AGAGGAGAGA GGACCGACAT 
CGCATGAATG GGACAATGGA GAGGCGATAC AGAGCGGAGC GGGACTCTTC CCCTGACAGC 
AACTACAGCC GGGACGAGCT GAACTATGCA GAAGCACCTA GACTGAAGGA CTACTACAGC 
ATGATGAAGA ACAACGCTGC ACACAACAAT GCTACCTACA ACAACGCAGG CCATAACAAT 
AACACAGTAG GCCGCAGCTC AAACCAGGTC CCTGAGCCCA AGAGGAGGCT GTACAAAGAA 
AGTCCTAAAG ACCTCAGCAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108232031 ( XM_017409522.1 ) cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus) -> XP_017265011.1 Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X5, mRNA.

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CloneID	OKr06772
Clone ID Related Accession (Same CDS sequence)	XM_017409522.1 , XM_017409522.2
Accession Version	XM_017409522.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-27
Organism	Kryptolebias marmoratus(mangrove rivulus)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016094253.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Kryptolebias marmoratus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACGA AAGTGAAGGA GTGCGCCGTG 
GTTCGGGACC CCAGCGGGGA GCTGAACTTG GAGCTGCGCG GCGGGGCGCA GGACGGAGAG 
TTCGCCTACA TCGGACACAT CAGGGAGGAT GCTGTGCTCC ACCAGGACGG GAAACTGTCT 
GAGGGAGAGC TGCTGCTGGA GGTGGAGGGG CTGTCCGTGT CTGGATTACC TCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAAGGT CCCGTCAGGT TCAAAACTGT CCGCCAAGGT 
CACAAGCTCA ACAAGGACCT AAAGCACTTC CTGAGCTTGA GGTTTCAGAA GTCATCACCT 
GACCACGAGT TGCAGAAGAC TATCCGAGCC AATCTATATC GTCATGCCGT GCCTTGTACA 
ACCCGCGCCC CTCGAGACGG AGAGGTGCCA GGGGTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTTGAGGA GAGTGGCACA CTACTGGAAA TAGGAACGTA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC GCCGAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
ACGGGCTCCC ATCAGTCCAC CCCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAACGTC 
CGCGTAGTGC CTGCTGACGA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGAACCT TAGTGCGTAG CCTTTTCCCC TCTCCTTTGT TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGTAC CGGCATCCTT CGGCCATATC CTGTGCGTGA TAATGCTCTG 
GCCTGTGGTC TGAACAATGC CACCACCTCC ACTGTTGAAA GTGGGCAGCA GACGCTCGCA 
GAACCACAGG TCTCTCCAGA TGACCCTCTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACTGAGA ACGGGGAGCT GTACTTCATA GACCACAACA CAAAGACCAC ATCATGGCTG 
GATCCGCGGT GCCGAGACAA AGCCTCCAGG CCTTTGGAAG AGTGTGATGA TGATGAAGAA 
GGGATACATA CGGAAGACCT GGAAAGTGAT CTAGAGCTGC CCCCAGGATG GGAAAGAATA 
GATGACCCCG TGTACGGAGT GTACTATGTA GATCATATAA ACAGGAAGAC CCAATATGAG 
AACCCAGTGG TGGAGGCGCG GCGCCAAAAA CTCTTGGAGC AGCAGCAACA GCCGCAGCCT 
CCAGAAGGTG AGCGGTATAT TCGAGAGTGG ATAGAGGAGC ACTCAGCTGC AGCAGCTCCT 
TTGGCAAACT ATGCTCCAAA CCACCTGGAG ACCTACAGGG ACCCACAAGT CCCTCCAGCT 
CTACCTCCAG GCCCCGCTGG CATCAAAAGA GGGAAACCTT TCTTTACGAG AAACCCAGCA 
GAGCTGAAGG GAACCTTCAT CAACACCAAG CTGAAGAAAA GTCACCGTGG ATTCGGGTTC 
ACCGTGGTGG GAGGTGACGA GCCCGACGAG TTCCTGCAGA TCAAGAGTCT GGTGCTGGAT 
GGGCCTGCGG CTGTTGATGG CAAGATGGAG ACAGGTGATG TGATTGTCAG CGTGAATGAC 
ACTATTGTGC TGGGCTACAC TCATGCTCAG GTGGTGAAGA TCTTCCAGTC CATCCCTATC 
GGCTCCATGG TGGACCTGGC CTTATGCCGT GGCTACCCAC TGCCCTTTGA CCCAGACGAC 
CCAAACACCA GCCTGGTGAC CTCTGTGGCC ATCTTGGACA AGGAACCCAT TATTGTCAAT 
GGGCAAGAGA CGTTTGACAC GCCGCCTAAC CAGGCTGGAC CCCTTAACAG CATGCGTGAG 
CCACGGCCTC TCAGCCCCTC AGCTGAGGTG GCGTCCAACG GCTCCCATGG TTACTCCAGC 
GACGTGGTCA CGTTGGCCTC GTCCATAGCC ACTCAGCCCG AGCTCATCAC CATCCACATG 
GAGAAGGGGG ACAAAGGTTT TGGCTTCACC ATCGCTGACA GCCTAACAGG CGGAGGCCAG 
AGGGTAAAGC AGATAGTGGA CTATCCACGG TGCCGCGGCC TGAAAGAGGG GGACATTCTA 
ATGGAGGTCA ACAAGAGAAA TGTCCAGAAT ATGAGTCACA ATCAGGTGGT GGATCTGCTG 
AGCAAATGTC CAAGAGGCAG TGAGGTCACC ATGTTGGTGC AAAGAGGAGT GGTGCCAGCC 
AAGAGGAGCC CAAAGCTGGT GCACCAACTG GAGAGAAAAG ACAGTCAGAA CAGCTCCCAG 
CACAGTGTGT GCAGCCATCG CAGCACCCAC ACCGATTCTC CCAGCCACCC ACCCTCTGCC 
ATGCCCAGTG AGGCAGTGGT CCCCACTCCC GCTGCCCCCA CTCAGCCTCT GCCAGTCTTG 
CCCCCCCAGG ACCCCGCAGA TGGCATGCTC ACCCTCCAGA AGAAGCCAGA CCCTTTTAAG 
ATCTGGGCAC AGTCCAGGAG CATGTATGAG AGTCGACTAC CAGATTGCCA GGAGCAAGAC 
ATTTTCCTTT GGCGGAAAGA TACAGGTTTT GGGTTTCGCA TCTTGGGAGG AAATGAGCCT 
GGGGAGCCTA TATACATAGG GCACATAGTG AAGTACGGGG CCGCAGATGA AGATGGCCGC 
CTGCGATCGG GTGACGAGCT CATCTGTGTG GACGGCACGG CTGTAGTGGG CAAATCTCAC 
CAGCTGGTGG TGCAGCTGAT GCAGCAGGCA GCCAAGCAGG GCCACGTCAA CCTCACTGTC 
AGACGCAAGA CCCCCGGATA CGGAGGGTCA AAAGGAGATG GTGACGTTCC TCCATCCCCA 
GCCTCCTCCC ACCACAGCAG CACCCAGGCA CCCAGTCTGA CGGAGGGAAA GCGCACCCCT 
CAGGGCAGCC AGAACTCACT CAACACTGTC AGCTCCGGCA GCGGATCCAC CAGCGGCATA 
GGCAGCGGCG GTGGAGGAGG CAGTGGGAGC GCGGTGGTGC CTGCCTCCCT GCAGCCTTAC 
GACGTGGAGA TCCAGCGAGG AGAGAACGAG GGATTTGGTT TTGTCATCGT GTCGTCCGTT 
TCCAGGCCTG ATGCTGGCAC TACTTTCGCT GGAAATGCTT GTGTGGCAAT GCCCCACAAA 
ATAGGACGCA TCATCGAGGG CAGCCCGGCG GACCGCTGCG GGAAGCTGAA GGTCGGGGAC 
AGAATACTGG CTGTTAACGG CTGCTCCATC ACCAACAAGT CTCACTCAGA CATCGTCAAC 
CTGATTAAGG AAGCCGGGAA CACCGTCTCA CTCAGGATCA TCCCAGGTGA TGAATCTTCC 
AATGCTTCTC TGCTGACTAA TGCTGAGAAG ATAGCTACCA TCACCACGAC ACACACACCT 
CATTCCACGG AGACCAGAAA TAATTCAAAG TCAAAAGGAG CTCCTCCTCC ACCCCCTTCA 
CAGACCCAAA CACAGGAAGC AGAGTTTTAT TCTGTGGACC TCGAGAGGGA CAGTAAAGGC 
TTCGGCTTCA GCTTAAGAGG AGGACGGGAG TACAACATGG ACCTGTACGT GTTGAGACTA 
GCAGAGGATG GGGCTGCTGT CAGAAATGGC AAGATGAGGG TGGGAGACGA GATCCTGGAG 
ATTAATGGTG AGAGCACAAA GAGCATGAAG CATTCACGAG CCATTGAACT GATCAAGAAC 
GGCGGTCGGA GGGCACGACT TGTCCTAAAA AGGGGCGATG GATCTGTACC TGAATATGAC 
GGCAGCAGTG ACGGGCACCC TCCCACACCC CGGGCACAAA ACACTCCGGA AGTGAGAACG 
TTGCCACCCA GCAGCCGATA TCACACCTCA TTGGAGTCCA GTTATCCACC AGACCTTCAC 
AAATCATCAC GCCTGGAGGA GGCTGCTTGT GGCCGAGGTA TGGACAAGAA CAGGGACAGG 
GCTGGTTCAG ATCAGATGGA ATTCCAAGGC CGACAATTTA ACCCCCATCA CCATTACACT 
TGGAATAATA ATCACAGTCA AAGTACCCCC AGACAGCAGT CCCCTGACAG TAATAGCACT 
AGCAGTGGCA ACAAGAAGAC CAGAGGCCGT AAAGTCAAGA AGTCTGAATC GGAGAGTGGC 
ATCTCTAAAC TTCTAAAGAC TCTGAGGAAG GACAGCTCGG GAAAAAAGGC TGCAGCCGCC 
GAAAAACTAA GGGGCCGAGA GCTTTCAAGC AGCGGTTCTA CTTTGGATGA GAAGTATCTT 
CGGCAGCGTC GTGGGTCCCT CGACCGCTCA CCAACCAGAA AGCGCACCTC GTCTCCAGAT 
CGTCGACGGG CGAAATCCAT GGACCGCAGG GCAGAGCGAG GACATCGTGA CCGCACACCT 
GAGAGGGAAC ACGATGATGG AGGGTTTCTC TCAGTCACCC CTGAACGGAG GTTCCACGAG 
CGAAGGCTCC TCAAGGACTC ACAGATAGCT GGACGAGTCA GTGAGGCTTC AACCCCCGAG 
CGCGCCAACA ACAGCATGGA ATCCTTGTCT CGTTCCAGGG GCCGCACGGA CGATAAAGCC 
ACAAAGAACG GCAACCTTCT GAGAGACTCT TCTTCAGAGA GGAGAGAGGA CCGACATCGC 
ATGAATGGGA CAATGGAGAG GCGATACAGA GCGGAGCGGG ACTCTTCCCC TGACAGCAAC 
TACAGCCGGG ACGAGCTGAA CTATGCAGAA GCACCTAGAC TGAAGGACTA CTACAGCATG 
ATGAAGAACA ACGCTGCACA CAACAATGCT ACCTACAACA ACGCAGGCCA TAACAATAAC 
ACAGTAGGCC GCAGCTCAAA CCAGGTCCCT GAGCCCAAGA GGAGGCTGTA CAAAGAAAGT 
CCTAAAGACC TCAGCATCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017265011.1
CDS	774..5594
Translation	MSKVIQKKNHWITKVKECAVVRDPSGELNLELRGGAQDGEFAYIGHIREDAVLHQDGKLSEGELLLEVEGLSVSGLPLYDIYTVINCCKGPVRFKTVRQGHKLNKDLKHFLSLRFQKSSPDHELQKTIRANLYRHAVPCTTRAPRDGEVPGVDYNFLSVEDFLQLEESGTLLEIGTYEGNYYGTPKPPRQPIIGTVTLSDTGSHQSTPKRTKSYNDMQNVRVVPADDDDDDQATEMNNSFTGTLVRSLFPSPLLCTGNPNDQDESRTGILRPYPVRDNALACGLNNATTSTVESGQQTLAEPQVSPDDPLGPLPENWEMAYTENGELYFIDHNTKTTSWLDPRCRDKASRPLEECDDDEEGIHTEDLESDLELPPGWERIDDPVYGVYYVDHINRKTQYENPVVEARRQKLLEQQQQPQPPEGERYIREWIEEHSAAAAPLANYAPNHLETYRDPQVPPALPPGPAGIKRGKPFFTRNPAELKGTFINTKLKKSHRGFGFTVVGGDEPDEFLQIKSLVLDGPAAVDGKMETGDVIVSVNDTIVLGYTHAQVVKIFQSIPIGSMVDLALCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETFDTPPNQAGPLNSMREPRPLSPSAEVASNGSHGYSSDVVTLASSIATQPELITIHMEKGDKGFGFTIADSLTGGGQRVKQIVDYPRCRGLKEGDILMEVNKRNVQNMSHNQVVDLLSKCPRGSEVTMLVQRGVVPAKRSPKLVHQLERKDSQNSSQHSVCSHRSTHTDSPSHPPSAMPSEAVVPTPAAPTQPLPVLPPQDPADGMLTLQKKPDPFKIWAQSRSMYESRLPDCQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTPGYGGSKGDGDVPPSPASSHHSSTQAPSLTEGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGSAVVPASLQPYDVEIQRGENEGFGFVIVSSVSRPDAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVSLRIIPGDESSNASLLTNAEKIATITTTHTPHSTETRNNSKSKGAPPPPPSQTQTQEAEFYSVDLERDSKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKSMKHSRAIELIKNGGRRARLVLKRGDGSVPEYDGSSDGHPPTPRAQNTPEVRTLPPSSRYHTSLESSYPPDLHKSSRLEEAACGRGMDKNRDRAGSDQMEFQGRQFNPHHHYTWNNNHSQSTPRQQSPDSNSTSSGNKKTRGRKVKKSESESGISKLLKTLRKDSSGKKAAAAEKLRGRELSSSGSTLDEKYLRQRRGSLDRSPTRKRTSSPDRRRAKSMDRRAERGHRDRTPEREHDDGGFLSVTPERRFHERRLLKDSQIAGRVSEASTPERANNSMESLSRSRGRTDDKATKNGNLLRDSSSERREDRHRMNGTMERRYRAERDSSPDSNYSRDELNYAEAPRLKDYYSMMKNNAAHNNATYNNAGHNNNTVGRSSNQVPEPKRRLYKESPKDLSI

Target ORF information:

RefSeq Version	XM_017409522.1
Organism	Kryptolebias marmoratus(mangrove rivulus)
Definition	Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017409522.1

ORF Insert Sequence:

ATGTCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACGA AAGTGAAGGA GTGCGCCGTG 
GTTCGGGACC CCAGCGGGGA GCTGAACTTG GAGCTGCGCG GCGGGGCGCA GGACGGAGAG 
TTCGCCTACA TCGGACACAT CAGGGAGGAT GCTGTGCTCC ACCAGGACGG GAAACTGTCT 
GAGGGAGAGC TGCTGCTGGA GGTGGAGGGG CTGTCCGTGT CTGGATTACC TCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAAGGT CCCGTCAGGT TCAAAACTGT CCGCCAAGGT 
CACAAGCTCA ACAAGGACCT AAAGCACTTC CTGAGCTTGA GGTTTCAGAA GTCATCACCT 
GACCACGAGT TGCAGAAGAC TATCCGAGCC AATCTATATC GTCATGCCGT GCCTTGTACA 
ACCCGCGCCC CTCGAGACGG AGAGGTGCCA GGGGTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTTGAGGA GAGTGGCACA CTACTGGAAA TAGGAACGTA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC GCCGAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
ACGGGCTCCC ATCAGTCCAC CCCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAACGTC 
CGCGTAGTGC CTGCTGACGA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGAACCT TAGTGCGTAG CCTTTTCCCC TCTCCTTTGT TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGTAC CGGCATCCTT CGGCCATATC CTGTGCGTGA TAATGCTCTG 
GCCTGTGGTC TGAACAATGC CACCACCTCC ACTGTTGAAA GTGGGCAGCA GACGCTCGCA 
GAACCACAGG TCTCTCCAGA TGACCCTCTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACTGAGA ACGGGGAGCT GTACTTCATA GACCACAACA CAAAGACCAC ATCATGGCTG 
GATCCGCGGT GCCGAGACAA AGCCTCCAGG CCTTTGGAAG AGTGTGATGA TGATGAAGAA 
GGGATACATA CGGAAGACCT GGAAAGTGAT CTAGAGCTGC CCCCAGGATG GGAAAGAATA 
GATGACCCCG TGTACGGAGT GTACTATGTA GATCATATAA ACAGGAAGAC CCAATATGAG 
AACCCAGTGG TGGAGGCGCG GCGCCAAAAA CTCTTGGAGC AGCAGCAACA GCCGCAGCCT 
CCAGAAGGTG AGCGGTATAT TCGAGAGTGG ATAGAGGAGC ACTCAGCTGC AGCAGCTCCT 
TTGGCAAACT ATGCTCCAAA CCACCTGGAG ACCTACAGGG ACCCACAAGT CCCTCCAGCT 
CTACCTCCAG GCCCCGCTGG CATCAAAAGA GGGAAACCTT TCTTTACGAG AAACCCAGCA 
GAGCTGAAGG GAACCTTCAT CAACACCAAG CTGAAGAAAA GTCACCGTGG ATTCGGGTTC 
ACCGTGGTGG GAGGTGACGA GCCCGACGAG TTCCTGCAGA TCAAGAGTCT GGTGCTGGAT 
GGGCCTGCGG CTGTTGATGG CAAGATGGAG ACAGGTGATG TGATTGTCAG CGTGAATGAC 
ACTATTGTGC TGGGCTACAC TCATGCTCAG GTGGTGAAGA TCTTCCAGTC CATCCCTATC 
GGCTCCATGG TGGACCTGGC CTTATGCCGT GGCTACCCAC TGCCCTTTGA CCCAGACGAC 
CCAAACACCA GCCTGGTGAC CTCTGTGGCC ATCTTGGACA AGGAACCCAT TATTGTCAAT 
GGGCAAGAGA CGTTTGACAC GCCGCCTAAC CAGGCTGGAC CCCTTAACAG CATGCGTGAG 
CCACGGCCTC TCAGCCCCTC AGCTGAGGTG GCGTCCAACG GCTCCCATGG TTACTCCAGC 
GACGTGGTCA CGTTGGCCTC GTCCATAGCC ACTCAGCCCG AGCTCATCAC CATCCACATG 
GAGAAGGGGG ACAAAGGTTT TGGCTTCACC ATCGCTGACA GCCTAACAGG CGGAGGCCAG 
AGGGTAAAGC AGATAGTGGA CTATCCACGG TGCCGCGGCC TGAAAGAGGG GGACATTCTA 
ATGGAGGTCA ACAAGAGAAA TGTCCAGAAT ATGAGTCACA ATCAGGTGGT GGATCTGCTG 
AGCAAATGTC CAAGAGGCAG TGAGGTCACC ATGTTGGTGC AAAGAGGAGT GGTGCCAGCC 
AAGAGGAGCC CAAAGCTGGT GCACCAACTG GAGAGAAAAG ACAGTCAGAA CAGCTCCCAG 
CACAGTGTGT GCAGCCATCG CAGCACCCAC ACCGATTCTC CCAGCCACCC ACCCTCTGCC 
ATGCCCAGTG AGGCAGTGGT CCCCACTCCC GCTGCCCCCA CTCAGCCTCT GCCAGTCTTG 
CCCCCCCAGG ACCCCGCAGA TGGCATGCTC ACCCTCCAGA AGAAGCCAGA CCCTTTTAAG 
ATCTGGGCAC AGTCCAGGAG CATGTATGAG AGTCGACTAC CAGATTGCCA GGAGCAAGAC 
ATTTTCCTTT GGCGGAAAGA TACAGGTTTT GGGTTTCGCA TCTTGGGAGG AAATGAGCCT 
GGGGAGCCTA TATACATAGG GCACATAGTG AAGTACGGGG CCGCAGATGA AGATGGCCGC 
CTGCGATCGG GTGACGAGCT CATCTGTGTG GACGGCACGG CTGTAGTGGG CAAATCTCAC 
CAGCTGGTGG TGCAGCTGAT GCAGCAGGCA GCCAAGCAGG GCCACGTCAA CCTCACTGTC 
AGACGCAAGA CCCCCGGATA CGGAGGGTCA AAAGGAGATG GTGACGTTCC TCCATCCCCA 
GCCTCCTCCC ACCACAGCAG CACCCAGGCA CCCAGTCTGA CGGAGGGAAA GCGCACCCCT 
CAGGGCAGCC AGAACTCACT CAACACTGTC AGCTCCGGCA GCGGATCCAC CAGCGGCATA 
GGCAGCGGCG GTGGAGGAGG CAGTGGGAGC GCGGTGGTGC CTGCCTCCCT GCAGCCTTAC 
GACGTGGAGA TCCAGCGAGG AGAGAACGAG GGATTTGGTT TTGTCATCGT GTCGTCCGTT 
TCCAGGCCTG ATGCTGGCAC TACTTTCGCT GGAAATGCTT GTGTGGCAAT GCCCCACAAA 
ATAGGACGCA TCATCGAGGG CAGCCCGGCG GACCGCTGCG GGAAGCTGAA GGTCGGGGAC 
AGAATACTGG CTGTTAACGG CTGCTCCATC ACCAACAAGT CTCACTCAGA CATCGTCAAC 
CTGATTAAGG AAGCCGGGAA CACCGTCTCA CTCAGGATCA TCCCAGGTGA TGAATCTTCC 
AATGCTTCTC TGCTGACTAA TGCTGAGAAG ATAGCTACCA TCACCACGAC ACACACACCT 
CATTCCACGG AGACCAGAAA TAATTCAAAG TCAAAAGGAG CTCCTCCTCC ACCCCCTTCA 
CAGACCCAAA CACAGGAAGC AGAGTTTTAT TCTGTGGACC TCGAGAGGGA CAGTAAAGGC 
TTCGGCTTCA GCTTAAGAGG AGGACGGGAG TACAACATGG ACCTGTACGT GTTGAGACTA 
GCAGAGGATG GGGCTGCTGT CAGAAATGGC AAGATGAGGG TGGGAGACGA GATCCTGGAG 
ATTAATGGTG AGAGCACAAA GAGCATGAAG CATTCACGAG CCATTGAACT GATCAAGAAC 
GGCGGTCGGA GGGCACGACT TGTCCTAAAA AGGGGCGATG GATCTGTACC TGAATATGAC 
GGCAGCAGTG ACGGGCACCC TCCCACACCC CGGGCACAAA ACACTCCGGA AGTGAGAACG 
TTGCCACCCA GCAGCCGATA TCACACCTCA TTGGAGTCCA GTTATCCACC AGACCTTCAC 
AAATCATCAC GCCTGGAGGA GGCTGCTTGT GGCCGAGGTA TGGACAAGAA CAGGGACAGG 
GCTGGTTCAG ATCAGATGGA ATTCCAAGGC CGACAATTTA ACCCCCATCA CCATTACACT 
TGGAATAATA ATCACAGTCA AAGTACCCCC AGACAGCAGT CCCCTGACAG TAATAGCACT 
AGCAGTGGCA ACAAGAAGAC CAGAGGCCGT AAAGTCAAGA AGTCTGAATC GGAGAGTGGC 
ATCTCTAAAC TTCTAAAGAC TCTGAGGAAG GACAGCTCGG GAAAAAAGGC TGCAGCCGCC 
GAAAAACTAA GGGGCCGAGA GCTTTCAAGC AGCGGTTCTA CTTTGGATGA GAAGTATCTT 
CGGCAGCGTC GTGGGTCCCT CGACCGCTCA CCAACCAGAA AGCGCACCTC GTCTCCAGAT 
CGTCGACGGG CGAAATCCAT GGACCGCAGG GCAGAGCGAG GACATCGTGA CCGCACACCT 
GAGAGGGAAC ACGATGATGG AGGGTTTCTC TCAGTCACCC CTGAACGGAG GTTCCACGAG 
CGAAGGCTCC TCAAGGACTC ACAGATAGCT GGACGAGTCA GTGAGGCTTC AACCCCCGAG 
CGCGCCAACA ACAGCATGGA ATCCTTGTCT CGTTCCAGGG GCCGCACGGA CGATAAAGCC 
ACAAAGAACG GCAACCTTCT GAGAGACTCT TCTTCAGAGA GGAGAGAGGA CCGACATCGC 
ATGAATGGGA CAATGGAGAG GCGATACAGA GCGGAGCGGG ACTCTTCCCC TGACAGCAAC 
TACAGCCGGG ACGAGCTGAA CTATGCAGAA GCACCTAGAC TGAAGGACTA CTACAGCATG 
ATGAAGAACA ACGCTGCACA CAACAATGCT ACCTACAACA ACGCAGGCCA TAACAATAAC 
ACAGTAGGCC GCAGCTCAAA CCAGGTCCCT GAGCCCAAGA GGAGGCTGTA CAAAGAAAGT 
CCTAAAGACC TCAGCATCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108232031 ( XM_017409522.2 ) cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus) -> XP_017265011.1 Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X6, mRNA.

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CloneID	OKr06772
Clone ID Related Accession (Same CDS sequence)	XM_017409522.1 , XM_017409522.2
Accession Version	XM_017409522.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-03
Organism	Kryptolebias marmoratus(mangrove rivulus)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016094253.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 4, 2018 this sequence version replaced XM_017409522.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Kryptolebias marmoratus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACGA AAGTGAAGGA GTGCGCCGTG 
GTTCGGGACC CCAGCGGGGA GCTGAACTTG GAGCTGCGCG GCGGGGCGCA GGACGGAGAG 
TTCGCCTACA TCGGACACAT CAGGGAGGAT GCTGTGCTCC ACCAGGACGG GAAACTGTCT 
GAGGGAGAGC TGCTGCTGGA GGTGGAGGGG CTGTCCGTGT CTGGATTACC TCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAAGGT CCCGTCAGGT TCAAAACTGT CCGCCAAGGT 
CACAAGCTCA ACAAGGACCT AAAGCACTTC CTGAGCTTGA GGTTTCAGAA GTCATCACCT 
GACCACGAGT TGCAGAAGAC TATCCGAGCC AATCTATATC GTCATGCCGT GCCTTGTACA 
ACCCGCGCCC CTCGAGACGG AGAGGTGCCA GGGGTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTTGAGGA GAGTGGCACA CTACTGGAAA TAGGAACGTA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC GCCGAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
ACGGGCTCCC ATCAGTCCAC CCCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAACGTC 
CGCGTAGTGC CTGCTGACGA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGAACCT TAGTGCGTAG CCTTTTCCCC TCTCCTTTGT TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGTAC CGGCATCCTT CGGCCATATC CTGTGCGTGA TAATGCTCTG 
GCCTGTGGTC TGAACAATGC CACCACCTCC ACTGTTGAAA GTGGGCAGCA GACGCTCGCA 
GAACCACAGG TCTCTCCAGA TGACCCTCTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACTGAGA ACGGGGAGCT GTACTTCATA GACCACAACA CAAAGACCAC ATCATGGCTG 
GATCCGCGGT GCCGAGACAA AGCCTCCAGG CCTTTGGAAG AGTGTGATGA TGATGAAGAA 
GGGATACATA CGGAAGACCT GGAAAGTGAT CTAGAGCTGC CCCCAGGATG GGAAAGAATA 
GATGACCCCG TGTACGGAGT GTACTATGTA GATCATATAA ACAGGAAGAC CCAATATGAG 
AACCCAGTGG TGGAGGCGCG GCGCCAAAAA CTCTTGGAGC AGCAGCAACA GCCGCAGCCT 
CCAGAAGGTG AGCGGTATAT TCGAGAGTGG ATAGAGGAGC ACTCAGCTGC AGCAGCTCCT 
TTGGCAAACT ATGCTCCAAA CCACCTGGAG ACCTACAGGG ACCCACAAGT CCCTCCAGCT 
CTACCTCCAG GCCCCGCTGG CATCAAAAGA GGGAAACCTT TCTTTACGAG AAACCCAGCA 
GAGCTGAAGG GAACCTTCAT CAACACCAAG CTGAAGAAAA GTCACCGTGG ATTCGGGTTC 
ACCGTGGTGG GAGGTGACGA GCCCGACGAG TTCCTGCAGA TCAAGAGTCT GGTGCTGGAT 
GGGCCTGCGG CTGTTGATGG CAAGATGGAG ACAGGTGATG TGATTGTCAG CGTGAATGAC 
ACTATTGTGC TGGGCTACAC TCATGCTCAG GTGGTGAAGA TCTTCCAGTC CATCCCTATC 
GGCTCCATGG TGGACCTGGC CTTATGCCGT GGCTACCCAC TGCCCTTTGA CCCAGACGAC 
CCAAACACCA GCCTGGTGAC CTCTGTGGCC ATCTTGGACA AGGAACCCAT TATTGTCAAT 
GGGCAAGAGA CGTTTGACAC GCCGCCTAAC CAGGCTGGAC CCCTTAACAG CATGCGTGAG 
CCACGGCCTC TCAGCCCCTC AGCTGAGGTG GCGTCCAACG GCTCCCATGG TTACTCCAGC 
GACGTGGTCA CGTTGGCCTC GTCCATAGCC ACTCAGCCCG AGCTCATCAC CATCCACATG 
GAGAAGGGGG ACAAAGGTTT TGGCTTCACC ATCGCTGACA GCCTAACAGG CGGAGGCCAG 
AGGGTAAAGC AGATAGTGGA CTATCCACGG TGCCGCGGCC TGAAAGAGGG GGACATTCTA 
ATGGAGGTCA ACAAGAGAAA TGTCCAGAAT ATGAGTCACA ATCAGGTGGT GGATCTGCTG 
AGCAAATGTC CAAGAGGCAG TGAGGTCACC ATGTTGGTGC AAAGAGGAGT GGTGCCAGCC 
AAGAGGAGCC CAAAGCTGGT GCACCAACTG GAGAGAAAAG ACAGTCAGAA CAGCTCCCAG 
CACAGTGTGT GCAGCCATCG CAGCACCCAC ACCGATTCTC CCAGCCACCC ACCCTCTGCC 
ATGCCCAGTG AGGCAGTGGT CCCCACTCCC GCTGCCCCCA CTCAGCCTCT GCCAGTCTTG 
CCCCCCCAGG ACCCCGCAGA TGGCATGCTC ACCCTCCAGA AGAAGCCAGA CCCTTTTAAG 
ATCTGGGCAC AGTCCAGGAG CATGTATGAG AGTCGACTAC CAGATTGCCA GGAGCAAGAC 
ATTTTCCTTT GGCGGAAAGA TACAGGTTTT GGGTTTCGCA TCTTGGGAGG AAATGAGCCT 
GGGGAGCCTA TATACATAGG GCACATAGTG AAGTACGGGG CCGCAGATGA AGATGGCCGC 
CTGCGATCGG GTGACGAGCT CATCTGTGTG GACGGCACGG CTGTAGTGGG CAAATCTCAC 
CAGCTGGTGG TGCAGCTGAT GCAGCAGGCA GCCAAGCAGG GCCACGTCAA CCTCACTGTC 
AGACGCAAGA CCCCCGGATA CGGAGGGTCA AAAGGAGATG GTGACGTTCC TCCATCCCCA 
GCCTCCTCCC ACCACAGCAG CACCCAGGCA CCCAGTCTGA CGGAGGGAAA GCGCACCCCT 
CAGGGCAGCC AGAACTCACT CAACACTGTC AGCTCCGGCA GCGGATCCAC CAGCGGCATA 
GGCAGCGGCG GTGGAGGAGG CAGTGGGAGC GCGGTGGTGC CTGCCTCCCT GCAGCCTTAC 
GACGTGGAGA TCCAGCGAGG AGAGAACGAG GGATTTGGTT TTGTCATCGT GTCGTCCGTT 
TCCAGGCCTG ATGCTGGCAC TACTTTCGCT GGAAATGCTT GTGTGGCAAT GCCCCACAAA 
ATAGGACGCA TCATCGAGGG CAGCCCGGCG GACCGCTGCG GGAAGCTGAA GGTCGGGGAC 
AGAATACTGG CTGTTAACGG CTGCTCCATC ACCAACAAGT CTCACTCAGA CATCGTCAAC 
CTGATTAAGG AAGCCGGGAA CACCGTCTCA CTCAGGATCA TCCCAGGTGA TGAATCTTCC 
AATGCTTCTC TGCTGACTAA TGCTGAGAAG ATAGCTACCA TCACCACGAC ACACACACCT 
CATTCCACGG AGACCAGAAA TAATTCAAAG TCAAAAGGAG CTCCTCCTCC ACCCCCTTCA 
CAGACCCAAA CACAGGAAGC AGAGTTTTAT TCTGTGGACC TCGAGAGGGA CAGTAAAGGC 
TTCGGCTTCA GCTTAAGAGG AGGACGGGAG TACAACATGG ACCTGTACGT GTTGAGACTA 
GCAGAGGATG GGGCTGCTGT CAGAAATGGC AAGATGAGGG TGGGAGACGA GATCCTGGAG 
ATTAATGGTG AGAGCACAAA GAGCATGAAG CATTCACGAG CCATTGAACT GATCAAGAAC 
GGCGGTCGGA GGGCACGACT TGTCCTAAAA AGGGGCGATG GATCTGTACC TGAATATGAC 
GGCAGCAGTG ACGGGCACCC TCCCACACCC CGGGCACAAA ACACTCCGGA AGTGAGAACG 
TTGCCACCCA GCAGCCGATA TCACACCTCA TTGGAGTCCA GTTATCCACC AGACCTTCAC 
AAATCATCAC GCCTGGAGGA GGCTGCTTGT GGCCGAGGTA TGGACAAGAA CAGGGACAGG 
GCTGGTTCAG ATCAGATGGA ATTCCAAGGC CGACAATTTA ACCCCCATCA CCATTACACT 
TGGAATAATA ATCACAGTCA AAGTACCCCC AGACAGCAGT CCCCTGACAG TAATAGCACT 
AGCAGTGGCA ACAAGAAGAC CAGAGGCCGT AAAGTCAAGA AGTCTGAATC GGAGAGTGGC 
ATCTCTAAAC TTCTAAAGAC TCTGAGGAAG GACAGCTCGG GAAAAAAGGC TGCAGCCGCC 
GAAAAACTAA GGGGCCGAGA GCTTTCAAGC AGCGGTTCTA CTTTGGATGA GAAGTATCTT 
CGGCAGCGTC GTGGGTCCCT CGACCGCTCA CCAACCAGAA AGCGCACCTC GTCTCCAGAT 
CGTCGACGGG CGAAATCCAT GGACCGCAGG GCAGAGCGAG GACATCGTGA CCGCACACCT 
GAGAGGGAAC ACGATGATGG AGGGTTTCTC TCAGTCACCC CTGAACGGAG GTTCCACGAG 
CGAAGGCTCC TCAAGGACTC ACAGATAGCT GGACGAGTCA GTGAGGCTTC AACCCCCGAG 
CGCGCCAACA ACAGCATGGA ATCCTTGTCT CGTTCCAGGG GCCGCACGGA CGATAAAGCC 
ACAAAGAACG GCAACCTTCT GAGAGACTCT TCTTCAGAGA GGAGAGAGGA CCGACATCGC 
ATGAATGGGA CAATGGAGAG GCGATACAGA GCGGAGCGGG ACTCTTCCCC TGACAGCAAC 
TACAGCCGGG ACGAGCTGAA CTATGCAGAA GCACCTAGAC TGAAGGACTA CTACAGCATG 
ATGAAGAACA ACGCTGCACA CAACAATGCT ACCTACAACA ACGCAGGCCA TAACAATAAC 
ACAGTAGGCC GCAGCTCAAA CCAGGTCCCT GAGCCCAAGA GGAGGCTGTA CAAAGAAAGT 
CCTAAAGACC TCAGCATCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017265011.1
CDS	892..5712
Translation	MSKVIQKKNHWITKVKECAVVRDPSGELNLELRGGAQDGEFAYIGHIREDAVLHQDGKLSEGELLLEVEGLSVSGLPLYDIYTVINCCKGPVRFKTVRQGHKLNKDLKHFLSLRFQKSSPDHELQKTIRANLYRHAVPCTTRAPRDGEVPGVDYNFLSVEDFLQLEESGTLLEIGTYEGNYYGTPKPPRQPIIGTVTLSDTGSHQSTPKRTKSYNDMQNVRVVPADDDDDDQATEMNNSFTGTLVRSLFPSPLLCTGNPNDQDESRTGILRPYPVRDNALACGLNNATTSTVESGQQTLAEPQVSPDDPLGPLPENWEMAYTENGELYFIDHNTKTTSWLDPRCRDKASRPLEECDDDEEGIHTEDLESDLELPPGWERIDDPVYGVYYVDHINRKTQYENPVVEARRQKLLEQQQQPQPPEGERYIREWIEEHSAAAAPLANYAPNHLETYRDPQVPPALPPGPAGIKRGKPFFTRNPAELKGTFINTKLKKSHRGFGFTVVGGDEPDEFLQIKSLVLDGPAAVDGKMETGDVIVSVNDTIVLGYTHAQVVKIFQSIPIGSMVDLALCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETFDTPPNQAGPLNSMREPRPLSPSAEVASNGSHGYSSDVVTLASSIATQPELITIHMEKGDKGFGFTIADSLTGGGQRVKQIVDYPRCRGLKEGDILMEVNKRNVQNMSHNQVVDLLSKCPRGSEVTMLVQRGVVPAKRSPKLVHQLERKDSQNSSQHSVCSHRSTHTDSPSHPPSAMPSEAVVPTPAAPTQPLPVLPPQDPADGMLTLQKKPDPFKIWAQSRSMYESRLPDCQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTPGYGGSKGDGDVPPSPASSHHSSTQAPSLTEGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGSAVVPASLQPYDVEIQRGENEGFGFVIVSSVSRPDAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVSLRIIPGDESSNASLLTNAEKIATITTTHTPHSTETRNNSKSKGAPPPPPSQTQTQEAEFYSVDLERDSKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKSMKHSRAIELIKNGGRRARLVLKRGDGSVPEYDGSSDGHPPTPRAQNTPEVRTLPPSSRYHTSLESSYPPDLHKSSRLEEAACGRGMDKNRDRAGSDQMEFQGRQFNPHHHYTWNNNHSQSTPRQQSPDSNSTSSGNKKTRGRKVKKSESESGISKLLKTLRKDSSGKKAAAAEKLRGRELSSSGSTLDEKYLRQRRGSLDRSPTRKRTSSPDRRRAKSMDRRAERGHRDRTPEREHDDGGFLSVTPERRFHERRLLKDSQIAGRVSEASTPERANNSMESLSRSRGRTDDKATKNGNLLRDSSSERREDRHRMNGTMERRYRAERDSSPDSNYSRDELNYAEAPRLKDYYSMMKNNAAHNNATYNNAGHNNNTVGRSSNQVPEPKRRLYKESPKDLSI

Target ORF information:

RefSeq Version	XM_017409522.2
Organism	Kryptolebias marmoratus(mangrove rivulus)
Definition	Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017409522.2

ORF Insert Sequence:

ATGTCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACGA AAGTGAAGGA GTGCGCCGTG 
GTTCGGGACC CCAGCGGGGA GCTGAACTTG GAGCTGCGCG GCGGGGCGCA GGACGGAGAG 
TTCGCCTACA TCGGACACAT CAGGGAGGAT GCTGTGCTCC ACCAGGACGG GAAACTGTCT 
GAGGGAGAGC TGCTGCTGGA GGTGGAGGGG CTGTCCGTGT CTGGATTACC TCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAAGGT CCCGTCAGGT TCAAAACTGT CCGCCAAGGT 
CACAAGCTCA ACAAGGACCT AAAGCACTTC CTGAGCTTGA GGTTTCAGAA GTCATCACCT 
GACCACGAGT TGCAGAAGAC TATCCGAGCC AATCTATATC GTCATGCCGT GCCTTGTACA 
ACCCGCGCCC CTCGAGACGG AGAGGTGCCA GGGGTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTTGAGGA GAGTGGCACA CTACTGGAAA TAGGAACGTA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC GCCGAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
ACGGGCTCCC ATCAGTCCAC CCCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAACGTC 
CGCGTAGTGC CTGCTGACGA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGAACCT TAGTGCGTAG CCTTTTCCCC TCTCCTTTGT TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGTAC CGGCATCCTT CGGCCATATC CTGTGCGTGA TAATGCTCTG 
GCCTGTGGTC TGAACAATGC CACCACCTCC ACTGTTGAAA GTGGGCAGCA GACGCTCGCA 
GAACCACAGG TCTCTCCAGA TGACCCTCTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACTGAGA ACGGGGAGCT GTACTTCATA GACCACAACA CAAAGACCAC ATCATGGCTG 
GATCCGCGGT GCCGAGACAA AGCCTCCAGG CCTTTGGAAG AGTGTGATGA TGATGAAGAA 
GGGATACATA CGGAAGACCT GGAAAGTGAT CTAGAGCTGC CCCCAGGATG GGAAAGAATA 
GATGACCCCG TGTACGGAGT GTACTATGTA GATCATATAA ACAGGAAGAC CCAATATGAG 
AACCCAGTGG TGGAGGCGCG GCGCCAAAAA CTCTTGGAGC AGCAGCAACA GCCGCAGCCT 
CCAGAAGGTG AGCGGTATAT TCGAGAGTGG ATAGAGGAGC ACTCAGCTGC AGCAGCTCCT 
TTGGCAAACT ATGCTCCAAA CCACCTGGAG ACCTACAGGG ACCCACAAGT CCCTCCAGCT 
CTACCTCCAG GCCCCGCTGG CATCAAAAGA GGGAAACCTT TCTTTACGAG AAACCCAGCA 
GAGCTGAAGG GAACCTTCAT CAACACCAAG CTGAAGAAAA GTCACCGTGG ATTCGGGTTC 
ACCGTGGTGG GAGGTGACGA GCCCGACGAG TTCCTGCAGA TCAAGAGTCT GGTGCTGGAT 
GGGCCTGCGG CTGTTGATGG CAAGATGGAG ACAGGTGATG TGATTGTCAG CGTGAATGAC 
ACTATTGTGC TGGGCTACAC TCATGCTCAG GTGGTGAAGA TCTTCCAGTC CATCCCTATC 
GGCTCCATGG TGGACCTGGC CTTATGCCGT GGCTACCCAC TGCCCTTTGA CCCAGACGAC 
CCAAACACCA GCCTGGTGAC CTCTGTGGCC ATCTTGGACA AGGAACCCAT TATTGTCAAT 
GGGCAAGAGA CGTTTGACAC GCCGCCTAAC CAGGCTGGAC CCCTTAACAG CATGCGTGAG 
CCACGGCCTC TCAGCCCCTC AGCTGAGGTG GCGTCCAACG GCTCCCATGG TTACTCCAGC 
GACGTGGTCA CGTTGGCCTC GTCCATAGCC ACTCAGCCCG AGCTCATCAC CATCCACATG 
GAGAAGGGGG ACAAAGGTTT TGGCTTCACC ATCGCTGACA GCCTAACAGG CGGAGGCCAG 
AGGGTAAAGC AGATAGTGGA CTATCCACGG TGCCGCGGCC TGAAAGAGGG GGACATTCTA 
ATGGAGGTCA ACAAGAGAAA TGTCCAGAAT ATGAGTCACA ATCAGGTGGT GGATCTGCTG 
AGCAAATGTC CAAGAGGCAG TGAGGTCACC ATGTTGGTGC AAAGAGGAGT GGTGCCAGCC 
AAGAGGAGCC CAAAGCTGGT GCACCAACTG GAGAGAAAAG ACAGTCAGAA CAGCTCCCAG 
CACAGTGTGT GCAGCCATCG CAGCACCCAC ACCGATTCTC CCAGCCACCC ACCCTCTGCC 
ATGCCCAGTG AGGCAGTGGT CCCCACTCCC GCTGCCCCCA CTCAGCCTCT GCCAGTCTTG 
CCCCCCCAGG ACCCCGCAGA TGGCATGCTC ACCCTCCAGA AGAAGCCAGA CCCTTTTAAG 
ATCTGGGCAC AGTCCAGGAG CATGTATGAG AGTCGACTAC CAGATTGCCA GGAGCAAGAC 
ATTTTCCTTT GGCGGAAAGA TACAGGTTTT GGGTTTCGCA TCTTGGGAGG AAATGAGCCT 
GGGGAGCCTA TATACATAGG GCACATAGTG AAGTACGGGG CCGCAGATGA AGATGGCCGC 
CTGCGATCGG GTGACGAGCT CATCTGTGTG GACGGCACGG CTGTAGTGGG CAAATCTCAC 
CAGCTGGTGG TGCAGCTGAT GCAGCAGGCA GCCAAGCAGG GCCACGTCAA CCTCACTGTC 
AGACGCAAGA CCCCCGGATA CGGAGGGTCA AAAGGAGATG GTGACGTTCC TCCATCCCCA 
GCCTCCTCCC ACCACAGCAG CACCCAGGCA CCCAGTCTGA CGGAGGGAAA GCGCACCCCT 
CAGGGCAGCC AGAACTCACT CAACACTGTC AGCTCCGGCA GCGGATCCAC CAGCGGCATA 
GGCAGCGGCG GTGGAGGAGG CAGTGGGAGC GCGGTGGTGC CTGCCTCCCT GCAGCCTTAC 
GACGTGGAGA TCCAGCGAGG AGAGAACGAG GGATTTGGTT TTGTCATCGT GTCGTCCGTT 
TCCAGGCCTG ATGCTGGCAC TACTTTCGCT GGAAATGCTT GTGTGGCAAT GCCCCACAAA 
ATAGGACGCA TCATCGAGGG CAGCCCGGCG GACCGCTGCG GGAAGCTGAA GGTCGGGGAC 
AGAATACTGG CTGTTAACGG CTGCTCCATC ACCAACAAGT CTCACTCAGA CATCGTCAAC 
CTGATTAAGG AAGCCGGGAA CACCGTCTCA CTCAGGATCA TCCCAGGTGA TGAATCTTCC 
AATGCTTCTC TGCTGACTAA TGCTGAGAAG ATAGCTACCA TCACCACGAC ACACACACCT 
CATTCCACGG AGACCAGAAA TAATTCAAAG TCAAAAGGAG CTCCTCCTCC ACCCCCTTCA 
CAGACCCAAA CACAGGAAGC AGAGTTTTAT TCTGTGGACC TCGAGAGGGA CAGTAAAGGC 
TTCGGCTTCA GCTTAAGAGG AGGACGGGAG TACAACATGG ACCTGTACGT GTTGAGACTA 
GCAGAGGATG GGGCTGCTGT CAGAAATGGC AAGATGAGGG TGGGAGACGA GATCCTGGAG 
ATTAATGGTG AGAGCACAAA GAGCATGAAG CATTCACGAG CCATTGAACT GATCAAGAAC 
GGCGGTCGGA GGGCACGACT TGTCCTAAAA AGGGGCGATG GATCTGTACC TGAATATGAC 
GGCAGCAGTG ACGGGCACCC TCCCACACCC CGGGCACAAA ACACTCCGGA AGTGAGAACG 
TTGCCACCCA GCAGCCGATA TCACACCTCA TTGGAGTCCA GTTATCCACC AGACCTTCAC 
AAATCATCAC GCCTGGAGGA GGCTGCTTGT GGCCGAGGTA TGGACAAGAA CAGGGACAGG 
GCTGGTTCAG ATCAGATGGA ATTCCAAGGC CGACAATTTA ACCCCCATCA CCATTACACT 
TGGAATAATA ATCACAGTCA AAGTACCCCC AGACAGCAGT CCCCTGACAG TAATAGCACT 
AGCAGTGGCA ACAAGAAGAC CAGAGGCCGT AAAGTCAAGA AGTCTGAATC GGAGAGTGGC 
ATCTCTAAAC TTCTAAAGAC TCTGAGGAAG GACAGCTCGG GAAAAAAGGC TGCAGCCGCC 
GAAAAACTAA GGGGCCGAGA GCTTTCAAGC AGCGGTTCTA CTTTGGATGA GAAGTATCTT 
CGGCAGCGTC GTGGGTCCCT CGACCGCTCA CCAACCAGAA AGCGCACCTC GTCTCCAGAT 
CGTCGACGGG CGAAATCCAT GGACCGCAGG GCAGAGCGAG GACATCGTGA CCGCACACCT 
GAGAGGGAAC ACGATGATGG AGGGTTTCTC TCAGTCACCC CTGAACGGAG GTTCCACGAG 
CGAAGGCTCC TCAAGGACTC ACAGATAGCT GGACGAGTCA GTGAGGCTTC AACCCCCGAG 
CGCGCCAACA ACAGCATGGA ATCCTTGTCT CGTTCCAGGG GCCGCACGGA CGATAAAGCC 
ACAAAGAACG GCAACCTTCT GAGAGACTCT TCTTCAGAGA GGAGAGAGGA CCGACATCGC 
ATGAATGGGA CAATGGAGAG GCGATACAGA GCGGAGCGGG ACTCTTCCCC TGACAGCAAC 
TACAGCCGGG ACGAGCTGAA CTATGCAGAA GCACCTAGAC TGAAGGACTA CTACAGCATG 
ATGAAGAACA ACGCTGCACA CAACAATGCT ACCTACAACA ACGCAGGCCA TAACAATAAC 
ACAGTAGGCC GCAGCTCAAA CCAGGTCCCT GAGCCCAAGA GGAGGCTGTA CAAAGAAAGT 
CCTAAAGACC TCAGCATCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108232031 ( XM_017409523.1 ) cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus) -> XP_017265012.1 Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X6, mRNA.

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CloneID	OKr06773
Clone ID Related Accession (Same CDS sequence)	XM_017409523.1
Accession Version	XM_017409523.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4773bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-27
Organism	Kryptolebias marmoratus(mangrove rivulus)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016094253.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Kryptolebias marmoratus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACGA AAGTGAAGGA GTGCGCCGTG 
GTTCGGGACC CCAGCGGGGA GCTGAACTTG GAGCTGCGCG GCGGGGCGCA GGACGGAGAG 
TTCGCCTACA TCGGACACAT CAGGGAGGAT GCTGTGCTCC ACCAGGACGG GAAACTGTCT 
GAGGGAGAGC TGCTGCTGGA GGTGGAGGGG CTGTCCGTGT CTGGATTACC TCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAAGGT CCCGTCAGGT TCAAAACTGT CCGCCAAGGT 
CACAAGCTCA ACAAGGACCT AAAGCACTTC CTGAGCTTGA GGTTTCAGAA GTCATCACCT 
GACCACGAGT TGCAGAAGAC TATCCGAGCC AATCTATATC GTCATGCCGT GCCTTGTACA 
ACCCGCGCCC CTCGAGACGG AGAGGTGCCA GGGGTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTTGAGGA GAGTGGCACA CTACTGGAAA TAGGAACGTA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC GCCGAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
ACGGGCTCCC ATCAGTCCAC CCCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAACGTC 
CGCGTAGTGC CTGCTGACGA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGTAACC CTAACGACCA GGACGAGAGC CGTACCGGCA TCCTTCGGCC ATATCCTGTG 
CGTGATAATG CTCTGGCCTG TGGTCTGAAC AATGCCACCA CCTCCACTGT TGAAAGTGGG 
CAGCAGACGC TCGCAGAACC ACAGGTCTCT CCAGATGACC CTCTGGGACC GCTGCCTGAA 
AACTGGGAGA TGGCCTACAC TGAGAACGGG GAGCTGTACT TCATAGACCA CAACACAAAG 
ACCACATCAT GGCTGGATCC GCGGTGCCGA GACAAAGCCT CCAGGCCTTT GGAAGAGTGT 
GATGATGATG AAGGGATACA TACGGAAGAC CTGGAAAGTG ATCTAGAGCT GCCCCCAGGA 
TGGGAAAGAA TAGATGACCC CGTGTACGGA GTGTACTATG TAGATCATAT AAACAGGAAG 
ACCCAATATG AGAACCCAGT GGTGGAGGCG CGGCGCCAAA AACTCTTGGA GCAGCAGCAA 
CAGCCGCAGC CTCCAGAAGG TGAGCGGTAT ATTCGAGAGT GGATAGAGGA GCACTCAGCT 
GCAGCAGCTC CTTTGGCAAA CTATGCTCCA AACCACCTGG AGACCTACAG GGACCCACAA 
GTCCCTCCAG CTCTACCTCC AGGCCCCGCT GGCATCAAAA GAGGGAAACC TTTCTTTACG 
AGAAACCCAG CAGAGCTGAA GGGAACCTTC ATCAACACCA AGCTGAAGAA AAGTCACCGT 
GGATTCGGGT TCACCGTGGT GGGAGGTGAC GAGCCCGACG AGTTCCTGCA GATCAAGAGT 
CTGGTGCTGG ATGGGCCTGC GGCTGTTGAT GGCAAGATGG AGACAGGTGA TGTGATTGTC 
AGCGTGAATG ACACTATTGT GCTGGGCTAC ACTCATGCTC AGGTGGTGAA GATCTTCCAG 
TCCATCCCTA TCGGCTCCAT GGTGGACCTG GCCTTATGCC GTGGCTACCC ACTGCCCTTT 
GACCCAGACG ACCCAAACAC CAGCCTGGTG ACCTCTGTGG CCATCTTGGA CAAGGAACCC 
ATTATTGTCA ATGGGCAAGA GACGTTTGAC ACGCCGCCTA ACCAGGCTGG ACCCCTTAAC 
AGCATGCGTG AGCCACGGCC TCTCAGCCCC TCAGCTGAGG TGGCGTCCAA CGGCTCCCAT 
GGTTACTCCA GCGACGTGGT CACGTTGGCC TCGTCCATAG CCACTCAGCC CGAGCTCATC 
ACCATCCACA TGGAGAAGGG GGACAAAGGT TTTGGCTTCA CCATCGCTGA CAGCCTAACA 
GGCGGAGGCC AGAGGGTAAA GCAGATAGTG GACTATCCAC GGTGCCGCGG CCTGAAAGAG 
GGGGACATTC TAATGGAGGT CAACAAGAGA AATGTCCAGA ATATGAGTCA CAATCAGGTG 
GTGGATCTGC TGAGCAAATG TCCAAGAGGC AGTGAGGTCA CCATGTTGGT GCAAAGAGGA 
GTGGTGCCAG CCAAGAGGAG CCCAAAGCTG GTGCACCAAC TGGAGAGAAA AGACAGTCAG 
AACAGCTCCC AGCACAGTGT GTGCAGCCAT CGCAGCACCC ACACCGATTC TCCCAGCCAC 
CCACCCTCTG CCATGCCCAG TGAGGCAGTG GTCCCCACTC CCGCTGCCCC CACTCAGCCT 
CTGCCAGTCT TGCCCCCCCA GGACCCCGCA GATGGCATGC TCACCCTCCA GAAGAAGCCA 
GACCCTTTTA AGATCTGGGC ACAGTCCAGG AGCATGTATG AGAGTCGACT ACCAGATTGC 
CAGGAGCAAG ACATTTTCCT TTGGCGGAAA GATACAGGTT TTGGGTTTCG CATCTTGGGA 
GGAAATGAGC CTGGGGAGCC TATATACATA GGGCACATAG TGAAGTACGG GGCCGCAGAT 
GAAGATGGCC GCCTGCGATC GGGTGACGAG CTCATCTGTG TGGACGGCAC GGCTGTAGTG 
GGCAAATCTC ACCAGCTGGT GGTGCAGCTG ATGCAGCAGG CAGCCAAGCA GGGCCACGTC 
AACCTCACTG TCAGACGCAA GACCCCCGGA TACGGAGGGT CAAAAGGAGA TGGTGACGTT 
CCTCCATCCC CAGCCTCCTC CCACCACAGC AGCACCCAGG CACCCAGTCT GACGGAGGGA 
AAGCGCACCC CTCAGGGCAG CCAGAACTCA CTCAACACTG TCAGCTCCGG CAGCGGATCC 
ACCAGCGGCA TAGGCAGCGG CGGTGGAGGA GGCAGTGGGA GCGCGGTGGT GCCTGCCTCC 
CTGCAGCCTT ACGACGTGGA GATCCAGCGA GGAGAGAACG AGGGATTTGG TTTTGTCATC 
GTGTCGTCCG TTTCCAGGCC TGATGCTGGC ACTACTTTCG CTGGAAATGC TTGTGTGGCA 
ATGCCCCACA AAATAGGACG CATCATCGAG GGCAGCCCGG CGGACCGCTG CGGGAAGCTG 
AAGGTCGGGG ACAGAATACT GGCTGTTAAC GGCTGCTCCA TCACCAACAA GTCTCACTCA 
GACATCGTCA ACCTGATTAA GGAAGCCGGG AACACCGTCT CACTCAGGAT CATCCCAGGT 
GATGAATCTT CCAATGCTTC TCTGCTGACT AATGCTGAGA AGATAGCTAC CATCACCACG 
ACACACACAC CTCATTCCAC GGAGACCAGA AATAATTCAA AGTCAAAAGG AGCTCCTCCT 
CCACCCCCTT CACAGACCCA AACACAGGAA GCAGAGTTTT ATTCTGTGGA CCTCGAGAGG 
GACAGTAAAG GCTTCGGCTT CAGCTTAAGA GGAGGACGGG AGTACAACAT GGACCTGTAC 
GTGTTGAGAC TAGCAGAGGA TGGGGCTGCT GTCAGAAATG GCAAGATGAG GGTGGGAGAC 
GAGATCCTGG AGATTAATGG TGAGAGCACA AAGAGCATGA AGCATTCACG AGCCATTGAA 
CTGATCAAGA ACGGCGGTCG GAGGGCACGA CTTGTCCTAA AAAGGGGCGA TGGATCTGTA 
CCTGAATATG ACGGCAGCAG TGACGGGCAC CCTCCCACAC CCCGGGCACA AAACACTCCG 
GAAGTGAGAA CGTTGCCACC CAGCAGCCGA TATCACACCT CATTGGAGTC CAGTTATCCA 
CCAGACCTTC ACAAATCATC ACGCCTGGAG GAGGCTGCTT GTGGCCGAGG TATGGACAAG 
AACAGGGACA GGGCTGGTTC AGATCAGATG GAATTCCAAG GCCGACAATT TAACCCCCAT 
CACCATTACA CTTGGAATAA TAATCACAGT CAAAGTACCC CCAGACAGCA GTCCCCTGAC 
AGTAATAGCA CTAGCAGTGG CAACAAGAAG ACCAGAGGCC GTAAAGTCAA GAAGTCTGAA 
TCGGAGAGTG GCATCTCTAA ACTTCTAAAG ACTCTGAGGA AGGACAGCTC GGGAAAAAAG 
GCTGCAGCCG CCGAAAAACT AAGGGGCCGA GAGCTTTCAA GCAGCGGTTC TACTTTGGAT 
GAGAAGTATC TTCGGCAGCG TCGTGGGTCC CTCGACCGCT CACCAACCAG AAAGCGCACC 
TCGTCTCCAG ATCGTCGACG GGCGAAATCC ATGGACCGCA GGGCAGAGCG AGGACATCGT 
GACCGCACAC CTGAGAGGGA ACACGATGAT GGAGGGTTTC TCTCAGTCAC CCCTGAACGG 
AGGTTCCACG AGCGAAGGCT CCTCAAGGAC TCACAGATAG CTGGACGAGT CAGTGAGGCT 
TCAACCCCCG AGCGCGCCAA CAACAGCATG GAATCCTTGT CTCGTTCCAG GGGCCGCACG 
GACGATAAAG CCACAAAGAA CGGCAACCTT CTGAGAGACT CTTCTTCAGA GAGGAGAGAG 
GACCGACATC GCATGAATGG GACAATGGAG AGGCGATACA GAGCGGAGCG GGACTCTTCC 
CCTGACAGCA ACTACAGCCG GGACGAGCTG AACTATGCAG AAGCACCTAG ACTGAAGGAC 
TACTACAGCA TGATGAAGAA CAACGCTGCA CACAACAATG CTACCTACAA CAACGCAGGC 
CATAACAATA ACACAGTAGG CCGCAGCTCA AACCAGGTCC CTGAGCCCAA GAGGAGGCTG 
TACAAAGAAA GTCCTAAAGA CCTCAGCATC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017265012.1
CDS	773..5545
Translation	MSKVIQKKNHWITKVKECAVVRDPSGELNLELRGGAQDGEFAYIGHIREDAVLHQDGKLSEGELLLEVEGLSVSGLPLYDIYTVINCCKGPVRFKTVRQGHKLNKDLKHFLSLRFQKSSPDHELQKTIRANLYRHAVPCTTRAPRDGEVPGVDYNFLSVEDFLQLEESGTLLEIGTYEGNYYGTPKPPRQPIIGTVTLSDTGSHQSTPKRTKSYNDMQNVRVVPADDDDDDQATEMNNSFTGNPNDQDESRTGILRPYPVRDNALACGLNNATTSTVESGQQTLAEPQVSPDDPLGPLPENWEMAYTENGELYFIDHNTKTTSWLDPRCRDKASRPLEECDDDEGIHTEDLESDLELPPGWERIDDPVYGVYYVDHINRKTQYENPVVEARRQKLLEQQQQPQPPEGERYIREWIEEHSAAAAPLANYAPNHLETYRDPQVPPALPPGPAGIKRGKPFFTRNPAELKGTFINTKLKKSHRGFGFTVVGGDEPDEFLQIKSLVLDGPAAVDGKMETGDVIVSVNDTIVLGYTHAQVVKIFQSIPIGSMVDLALCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETFDTPPNQAGPLNSMREPRPLSPSAEVASNGSHGYSSDVVTLASSIATQPELITIHMEKGDKGFGFTIADSLTGGGQRVKQIVDYPRCRGLKEGDILMEVNKRNVQNMSHNQVVDLLSKCPRGSEVTMLVQRGVVPAKRSPKLVHQLERKDSQNSSQHSVCSHRSTHTDSPSHPPSAMPSEAVVPTPAAPTQPLPVLPPQDPADGMLTLQKKPDPFKIWAQSRSMYESRLPDCQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTPGYGGSKGDGDVPPSPASSHHSSTQAPSLTEGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGSAVVPASLQPYDVEIQRGENEGFGFVIVSSVSRPDAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVSLRIIPGDESSNASLLTNAEKIATITTTHTPHSTETRNNSKSKGAPPPPPSQTQTQEAEFYSVDLERDSKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKSMKHSRAIELIKNGGRRARLVLKRGDGSVPEYDGSSDGHPPTPRAQNTPEVRTLPPSSRYHTSLESSYPPDLHKSSRLEEAACGRGMDKNRDRAGSDQMEFQGRQFNPHHHYTWNNNHSQSTPRQQSPDSNSTSSGNKKTRGRKVKKSESESGISKLLKTLRKDSSGKKAAAAEKLRGRELSSSGSTLDEKYLRQRRGSLDRSPTRKRTSSPDRRRAKSMDRRAERGHRDRTPEREHDDGGFLSVTPERRFHERRLLKDSQIAGRVSEASTPERANNSMESLSRSRGRTDDKATKNGNLLRDSSSERREDRHRMNGTMERRYRAERDSSPDSNYSRDELNYAEAPRLKDYYSMMKNNAAHNNATYNNAGHNNNTVGRSSNQVPEPKRRLYKESPKDLSI

Target ORF information:

RefSeq Version	XM_017409523.1
Organism	Kryptolebias marmoratus(mangrove rivulus)
Definition	Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017409523.1

ORF Insert Sequence:

ATGTCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACGA AAGTGAAGGA GTGCGCCGTG 
GTTCGGGACC CCAGCGGGGA GCTGAACTTG GAGCTGCGCG GCGGGGCGCA GGACGGAGAG 
TTCGCCTACA TCGGACACAT CAGGGAGGAT GCTGTGCTCC ACCAGGACGG GAAACTGTCT 
GAGGGAGAGC TGCTGCTGGA GGTGGAGGGG CTGTCCGTGT CTGGATTACC TCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAAGGT CCCGTCAGGT TCAAAACTGT CCGCCAAGGT 
CACAAGCTCA ACAAGGACCT AAAGCACTTC CTGAGCTTGA GGTTTCAGAA GTCATCACCT 
GACCACGAGT TGCAGAAGAC TATCCGAGCC AATCTATATC GTCATGCCGT GCCTTGTACA 
ACCCGCGCCC CTCGAGACGG AGAGGTGCCA GGGGTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTTGAGGA GAGTGGCACA CTACTGGAAA TAGGAACGTA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC GCCGAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
ACGGGCTCCC ATCAGTCCAC CCCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAACGTC 
CGCGTAGTGC CTGCTGACGA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGTAACC CTAACGACCA GGACGAGAGC CGTACCGGCA TCCTTCGGCC ATATCCTGTG 
CGTGATAATG CTCTGGCCTG TGGTCTGAAC AATGCCACCA CCTCCACTGT TGAAAGTGGG 
CAGCAGACGC TCGCAGAACC ACAGGTCTCT CCAGATGACC CTCTGGGACC GCTGCCTGAA 
AACTGGGAGA TGGCCTACAC TGAGAACGGG GAGCTGTACT TCATAGACCA CAACACAAAG 
ACCACATCAT GGCTGGATCC GCGGTGCCGA GACAAAGCCT CCAGGCCTTT GGAAGAGTGT 
GATGATGATG AAGGGATACA TACGGAAGAC CTGGAAAGTG ATCTAGAGCT GCCCCCAGGA 
TGGGAAAGAA TAGATGACCC CGTGTACGGA GTGTACTATG TAGATCATAT AAACAGGAAG 
ACCCAATATG AGAACCCAGT GGTGGAGGCG CGGCGCCAAA AACTCTTGGA GCAGCAGCAA 
CAGCCGCAGC CTCCAGAAGG TGAGCGGTAT ATTCGAGAGT GGATAGAGGA GCACTCAGCT 
GCAGCAGCTC CTTTGGCAAA CTATGCTCCA AACCACCTGG AGACCTACAG GGACCCACAA 
GTCCCTCCAG CTCTACCTCC AGGCCCCGCT GGCATCAAAA GAGGGAAACC TTTCTTTACG 
AGAAACCCAG CAGAGCTGAA GGGAACCTTC ATCAACACCA AGCTGAAGAA AAGTCACCGT 
GGATTCGGGT TCACCGTGGT GGGAGGTGAC GAGCCCGACG AGTTCCTGCA GATCAAGAGT 
CTGGTGCTGG ATGGGCCTGC GGCTGTTGAT GGCAAGATGG AGACAGGTGA TGTGATTGTC 
AGCGTGAATG ACACTATTGT GCTGGGCTAC ACTCATGCTC AGGTGGTGAA GATCTTCCAG 
TCCATCCCTA TCGGCTCCAT GGTGGACCTG GCCTTATGCC GTGGCTACCC ACTGCCCTTT 
GACCCAGACG ACCCAAACAC CAGCCTGGTG ACCTCTGTGG CCATCTTGGA CAAGGAACCC 
ATTATTGTCA ATGGGCAAGA GACGTTTGAC ACGCCGCCTA ACCAGGCTGG ACCCCTTAAC 
AGCATGCGTG AGCCACGGCC TCTCAGCCCC TCAGCTGAGG TGGCGTCCAA CGGCTCCCAT 
GGTTACTCCA GCGACGTGGT CACGTTGGCC TCGTCCATAG CCACTCAGCC CGAGCTCATC 
ACCATCCACA TGGAGAAGGG GGACAAAGGT TTTGGCTTCA CCATCGCTGA CAGCCTAACA 
GGCGGAGGCC AGAGGGTAAA GCAGATAGTG GACTATCCAC GGTGCCGCGG CCTGAAAGAG 
GGGGACATTC TAATGGAGGT CAACAAGAGA AATGTCCAGA ATATGAGTCA CAATCAGGTG 
GTGGATCTGC TGAGCAAATG TCCAAGAGGC AGTGAGGTCA CCATGTTGGT GCAAAGAGGA 
GTGGTGCCAG CCAAGAGGAG CCCAAAGCTG GTGCACCAAC TGGAGAGAAA AGACAGTCAG 
AACAGCTCCC AGCACAGTGT GTGCAGCCAT CGCAGCACCC ACACCGATTC TCCCAGCCAC 
CCACCCTCTG CCATGCCCAG TGAGGCAGTG GTCCCCACTC CCGCTGCCCC CACTCAGCCT 
CTGCCAGTCT TGCCCCCCCA GGACCCCGCA GATGGCATGC TCACCCTCCA GAAGAAGCCA 
GACCCTTTTA AGATCTGGGC ACAGTCCAGG AGCATGTATG AGAGTCGACT ACCAGATTGC 
CAGGAGCAAG ACATTTTCCT TTGGCGGAAA GATACAGGTT TTGGGTTTCG CATCTTGGGA 
GGAAATGAGC CTGGGGAGCC TATATACATA GGGCACATAG TGAAGTACGG GGCCGCAGAT 
GAAGATGGCC GCCTGCGATC GGGTGACGAG CTCATCTGTG TGGACGGCAC GGCTGTAGTG 
GGCAAATCTC ACCAGCTGGT GGTGCAGCTG ATGCAGCAGG CAGCCAAGCA GGGCCACGTC 
AACCTCACTG TCAGACGCAA GACCCCCGGA TACGGAGGGT CAAAAGGAGA TGGTGACGTT 
CCTCCATCCC CAGCCTCCTC CCACCACAGC AGCACCCAGG CACCCAGTCT GACGGAGGGA 
AAGCGCACCC CTCAGGGCAG CCAGAACTCA CTCAACACTG TCAGCTCCGG CAGCGGATCC 
ACCAGCGGCA TAGGCAGCGG CGGTGGAGGA GGCAGTGGGA GCGCGGTGGT GCCTGCCTCC 
CTGCAGCCTT ACGACGTGGA GATCCAGCGA GGAGAGAACG AGGGATTTGG TTTTGTCATC 
GTGTCGTCCG TTTCCAGGCC TGATGCTGGC ACTACTTTCG CTGGAAATGC TTGTGTGGCA 
ATGCCCCACA AAATAGGACG CATCATCGAG GGCAGCCCGG CGGACCGCTG CGGGAAGCTG 
AAGGTCGGGG ACAGAATACT GGCTGTTAAC GGCTGCTCCA TCACCAACAA GTCTCACTCA 
GACATCGTCA ACCTGATTAA GGAAGCCGGG AACACCGTCT CACTCAGGAT CATCCCAGGT 
GATGAATCTT CCAATGCTTC TCTGCTGACT AATGCTGAGA AGATAGCTAC CATCACCACG 
ACACACACAC CTCATTCCAC GGAGACCAGA AATAATTCAA AGTCAAAAGG AGCTCCTCCT 
CCACCCCCTT CACAGACCCA AACACAGGAA GCAGAGTTTT ATTCTGTGGA CCTCGAGAGG 
GACAGTAAAG GCTTCGGCTT CAGCTTAAGA GGAGGACGGG AGTACAACAT GGACCTGTAC 
GTGTTGAGAC TAGCAGAGGA TGGGGCTGCT GTCAGAAATG GCAAGATGAG GGTGGGAGAC 
GAGATCCTGG AGATTAATGG TGAGAGCACA AAGAGCATGA AGCATTCACG AGCCATTGAA 
CTGATCAAGA ACGGCGGTCG GAGGGCACGA CTTGTCCTAA AAAGGGGCGA TGGATCTGTA 
CCTGAATATG ACGGCAGCAG TGACGGGCAC CCTCCCACAC CCCGGGCACA AAACACTCCG 
GAAGTGAGAA CGTTGCCACC CAGCAGCCGA TATCACACCT CATTGGAGTC CAGTTATCCA 
CCAGACCTTC ACAAATCATC ACGCCTGGAG GAGGCTGCTT GTGGCCGAGG TATGGACAAG 
AACAGGGACA GGGCTGGTTC AGATCAGATG GAATTCCAAG GCCGACAATT TAACCCCCAT 
CACCATTACA CTTGGAATAA TAATCACAGT CAAAGTACCC CCAGACAGCA GTCCCCTGAC 
AGTAATAGCA CTAGCAGTGG CAACAAGAAG ACCAGAGGCC GTAAAGTCAA GAAGTCTGAA 
TCGGAGAGTG GCATCTCTAA ACTTCTAAAG ACTCTGAGGA AGGACAGCTC GGGAAAAAAG 
GCTGCAGCCG CCGAAAAACT AAGGGGCCGA GAGCTTTCAA GCAGCGGTTC TACTTTGGAT 
GAGAAGTATC TTCGGCAGCG TCGTGGGTCC CTCGACCGCT CACCAACCAG AAAGCGCACC 
TCGTCTCCAG ATCGTCGACG GGCGAAATCC ATGGACCGCA GGGCAGAGCG AGGACATCGT 
GACCGCACAC CTGAGAGGGA ACACGATGAT GGAGGGTTTC TCTCAGTCAC CCCTGAACGG 
AGGTTCCACG AGCGAAGGCT CCTCAAGGAC TCACAGATAG CTGGACGAGT CAGTGAGGCT 
TCAACCCCCG AGCGCGCCAA CAACAGCATG GAATCCTTGT CTCGTTCCAG GGGCCGCACG 
GACGATAAAG CCACAAAGAA CGGCAACCTT CTGAGAGACT CTTCTTCAGA GAGGAGAGAG 
GACCGACATC GCATGAATGG GACAATGGAG AGGCGATACA GAGCGGAGCG GGACTCTTCC 
CCTGACAGCA ACTACAGCCG GGACGAGCTG AACTATGCAG AAGCACCTAG ACTGAAGGAC 
TACTACAGCA TGATGAAGAA CAACGCTGCA CACAACAATG CTACCTACAA CAACGCAGGC 
CATAACAATA ACACAGTAGG CCGCAGCTCA AACCAGGTCC CTGAGCCCAA GAGGAGGCTG 
TACAAAGAAA GTCCTAAAGA CCTCAGCATC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108232031 ( XM_017409524.2 ) cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus) -> XP_017265013.1 Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X8, mRNA.

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CloneID	OKr06774
Clone ID Related Accession (Same CDS sequence)	XM_017409524.2 , XM_017409524.1
Accession Version	XM_017409524.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-03
Organism	Kryptolebias marmoratus(mangrove rivulus)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016094253.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 4, 2018 this sequence version replaced XM_017409524.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Kryptolebias marmoratus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACGA AAGTGAAGGA GTGCGCCGTG 
GTTCGGGACC CCAGCGGGGA GCTGAACTTG GAGCTGCGCG GCGGGGCGCA GGACGGAGAG 
TTCGCCTACA TCGGACACAT CAGGGAGGAT GCTGTGCTCC ACCAGGACGG GAAACTGTCT 
GAGGGAGAGC TGCTGCTGGA GGTGGAGGGG CTGTCCGTGT CTGGATTACC TCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAAGGT CCCGTCAGGT TCAAAACTGT CCGCCAAGGT 
CACAAGCTCA ACAAGGACCT AAAGCACTTC CTGAGCTTGA GGTTTCAGAA GTCATCACCT 
GACCACGAGT TGCAGAAGAC TATCCGAGCC AATCTATATC GTCATGCCGT GCCTTGTACA 
ACCCGCGCCC CTCGAGACGG AGAGGTGCCA GGGGTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTTGAGGA GAGTGGCACA CTACTGGAAA TAGGAACGTA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC GCCGAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
ACGGGCTCCC ATCAGTCCAC CCCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAACGTC 
CGCGTAGTGC CTGCTGACGA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGTAACC CTAACGACCA GGACGAGAGC CGTACCGGCA TCCTTCGGCC ATATCCTGTG 
CGTGATAATG CTCTGGCCTG TGGTCTGAAC AATGCCACCA CCTCCACTGT TGAAAGTGGG 
CAGCAGACGC TCGCAGAACC ACAGGTCTCT CCAGATGACC CTCTGGGACC GCTGCCTGAA 
AACTGGGAGA TGGCCTACAC TGAGAACGGG GAGCTGTACT TCATAGACCA CAACACAAAG 
ACCACATCAT GGCTGGATCC GCGGTGCCGA GACAAAGCCT CCAGGCCTTT GGAAGAGTGT 
GATGATGATG AAGGGATACA TACGGAAGAC CTGGAAAGTG ATCTAGAGCT GCCCCCAGGA 
TGGGAAAGAA TAGATGACCC CGTGTACGGA GTGTACTATG TAGATCATAT AAACAGGAAG 
ACCCAATATG AGAACCCAGT GGTGGAGGCG CGGCGCCAAA AACTCTTGGA GCAGCAGCAA 
CAGCCGCAGC CTCCAGAAGA GTGGATAGAG GAGCACTCAG CTGCAGCAGC TCCTTTGGCA 
AACTATGCTC CAAACCACCT GGAGACCTAC AGGGACCCAC AAGTCCCTCC AGCTCTACCT 
CCAGGCCCCG CTGGCATCAA AAGAGGGAAA CCTTTCTTTA CGAGAAACCC AGCAGAGCTG 
AAGGGAACCT TCATCAACAC CAAGCTGAAG AAAAGTCACC GTGGATTCGG GTTCACCGTG 
GTGGGAGGTG ACGAGCCCGA CGAGTTCCTG CAGATCAAGA GTCTGGTGCT GGATGGGCCT 
GCGGCTGTTG ATGGCAAGAT GGAGACAGGT GATGTGATTG TCAGCGTGAA TGACACTATT 
GTGCTGGGCT ACACTCATGC TCAGGTGGTG AAGATCTTCC AGTCCATCCC TATCGGCTCC 
ATGGTGGACC TGGCCTTATG CCGTGGCTAC CCACTGCCCT TTGACCCAGA CGACCCAAAC 
ACCAGCCTGG TGACCTCTGT GGCCATCTTG GACAAGGAAC CCATTATTGT CAATGGGCAA 
GAGACGTTTG ACACGCCGCC TAACCAGGCT GGACCCCTTA ACAGCATGCG TGAGCCACGG 
CCTCTCAGCC CCTCAGCTGA GGTGGCGTCC AACGGCTCCC ATGGTTACTC CAGCGACGTG 
GTCACGTTGG CCTCGTCCAT AGCCACTCAG CCCGAGCTCA TCACCATCCA CATGGAGAAG 
GGGGACAAAG GTTTTGGCTT CACCATCGCT GACAGCCTAA CAGGCGGAGG CCAGAGGGTA 
AAGCAGATAG TGGACTATCC ACGGTGCCGC GGCCTGAAAG AGGGGGACAT TCTAATGGAG 
GTCAACAAGA GAAATGTCCA GAATATGAGT CACAATCAGG TGGTGGATCT GCTGAGCAAA 
TGTCCAAGAG GCAGTGAGGT CACCATGTTG GTGCAAAGAG GAGTGGTGCC AGCCAAGAGG 
AGCCCAAAGC TGGTGCACCA ACTGGAGAGA AAAGACAGTC AGAACAGCTC CCAGCACAGT 
GTGTGCAGCC ATCGCAGCAC CCACACCGAT TCTCCCAGCC ACCCACCCTC TGCCATGCCC 
AGTGAGGCAG TGGTCCCCAC TCCCGCTGCC CCCACTCAGC CTCTGCCAGT CTTGCCCCCC 
CAGGACCCCG CAGATGGCAT GCTCACCCTC CAGAAGAAGC CAGACCCTTT TAAGATCTGG 
GCACAGTCCA GGAGCATGTA TGAGAGTCGA CTACCAGATT GCCAGGAGCA AGACATTTTC 
CTTTGGCGGA AAGATACAGG TTTTGGGTTT CGCATCTTGG GAGGAAATGA GCCTGGGGAG 
CCTATATACA TAGGGCACAT AGTGAAGTAC GGGGCCGCAG ATGAAGATGG CCGCCTGCGA 
TCGGGTGACG AGCTCATCTG TGTGGACGGC ACGGCTGTAG TGGGCAAATC TCACCAGCTG 
GTGGTGCAGC TGATGCAGCA GGCAGCCAAG CAGGGCCACG TCAACCTCAC TGTCAGACGC 
AAGACCCCCG GATACGGAGG GTCAAAAGGA GATGGTGACG TTCCTCCATC CCCAGCCTCC 
TCCCACCACA GCAGCACCCA GGCACCCAGT CTGACGGAGG GAAAGCGCAC CCCTCAGGGC 
AGCCAGAACT CACTCAACAC TGTCAGCTCC GGCAGCGGAT CCACCAGCGG CATAGGCAGC 
GGCGGTGGAG GAGGCAGTGG GAGCGCGGTG GTGCCTGCCT CCCTGCAGCC TTACGACGTG 
GAGATCCAGC GAGGAGAGAA CGAGGGATTT GGTTTTGTCA TCGTGTCGTC CGTTTCCAGG 
CCTGATGCTG GCACTACTTT CGCTGGAAAT GCTTGTGTGG CAATGCCCCA CAAAATAGGA 
CGCATCATCG AGGGCAGCCC GGCGGACCGC TGCGGGAAGC TGAAGGTCGG GGACAGAATA 
CTGGCTGTTA ACGGCTGCTC CATCACCAAC AAGTCTCACT CAGACATCGT CAACCTGATT 
AAGGAAGCCG GGAACACCGT CTCACTCAGG ATCATCCCAG GTGATGAATC TTCCAATGCT 
TCTCTGCTGA CTAATGCTGA GAAGATAGCT ACCATCACCA CGACACACAC ACCTCATTCC 
ACGGAGACCA GAAATAATTC AAAGTCAAAA GGAGCTCCTC CTCCACCCCC TTCACAGACC 
CAAACACAGG AAGCAGAGTT TTATTCTGTG GACCTCGAGA GGGACAGTAA AGGCTTCGGC 
TTCAGCTTAA GAGGAGGACG GGAGTACAAC ATGGACCTGT ACGTGTTGAG ACTAGCAGAG 
GATGGGGCTG CTGTCAGAAA TGGCAAGATG AGGGTGGGAG ACGAGATCCT GGAGATTAAT 
GGTGAGAGCA CAAAGAGCAT GAAGCATTCA CGAGCCATTG AACTGATCAA GAACGGCGGT 
CGGAGGGCAC GACTTGTCCT AAAAAGGGGC GATGGATCTG TACCTGAATA TGACGGCAGC 
AGTGACGGGC ACCCTCCCAC ACCCCGGGCA CAAAACACTC CGGAAGTGAG AACGTTGCCA 
CCCAGCAGCC GATATCACAC CTCATTGGAG TCCAGTTATC CACCAGACCT TCACAAATCA 
TCACGCCTGG AGGAGGCTGC TTGTGGCCGA GGTATGGACA AGAACAGGGA CAGGGCTGGT 
TCAGATCAGA TGGAATTCCA AGGCCGACAA TTTAACCCCC ATCACCATTA CACTTGGAAT 
AATAATCACA GTCAAAGTAC CCCCAGACAG CAGTCCCCTG ACAGTAATAG CACTAGCAGT 
GGCAACAAGA AGACCAGAGG CCGTAAAGTC AAGAAGTCTG AATCGGAGAG TGGCATCTCT 
AAACTTCTAA AGACTCTGAG GAAGGACAGC TCGGGAAAAA AGGCTGCAGC CGCCGAAAAA 
CTAAGGGGCC GAGAGCTTTC AAGCAGCGGT TCTACTTTGG ATGAGAAGTA TCTTCGGCAG 
CGTCGTGGGT CCCTCGACCG CTCACCAACC AGAAAGCGCA CCTCGTCTCC AGATCGTCGA 
CGGGCGAAAT CCATGGACCG CAGGGCAGAG CGAGGACATC GTGACCGCAC ACCTGAGAGG 
GAACACGATG ATGGAGGGTT TCTCTCAGTC ACCCCTGAAC GGAGGTTCCA CGAGCGAAGG 
CTCCTCAAGG ACTCACAGAT AGCTGGACGA GTCAGTGAGG CTTCAACCCC CGAGCGCGCC 
AACAACAGCA TGGAATCCTT GTCTCGTTCC AGGGGCCGCA CGGACGATAA AGCCACAAAG 
AACGGCAACC TTCTGAGAGA CTCTTCTTCA GAGAGGAGAG AGGACCGACA TCGCATGAAT 
GGGACAATGG AGAGGCGATA CAGAGCGGAG CGGGACTCTT CCCCTGACAG CAACTACAGC 
CGGGACGAGC TGAACTATGC AGAAGCACCT AGACTGAAGG ACTACTACAG CATGATGAAG 
AACAACGCTG CACACAACAA TGCTACCTAC AACAACGCAG GCCATAACAA TAACACAGTA 
GGCCGCAGCT CAAACCAGGT CCCTGAGCCC AAGAGGAGGC TGTACAAAGA AAGTCCTAAA 
GACCTCAGCA TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017265013.1
CDS	890..5644
Translation	MSKVIQKKNHWITKVKECAVVRDPSGELNLELRGGAQDGEFAYIGHIREDAVLHQDGKLSEGELLLEVEGLSVSGLPLYDIYTVINCCKGPVRFKTVRQGHKLNKDLKHFLSLRFQKSSPDHELQKTIRANLYRHAVPCTTRAPRDGEVPGVDYNFLSVEDFLQLEESGTLLEIGTYEGNYYGTPKPPRQPIIGTVTLSDTGSHQSTPKRTKSYNDMQNVRVVPADDDDDDQATEMNNSFTGNPNDQDESRTGILRPYPVRDNALACGLNNATTSTVESGQQTLAEPQVSPDDPLGPLPENWEMAYTENGELYFIDHNTKTTSWLDPRCRDKASRPLEECDDDEGIHTEDLESDLELPPGWERIDDPVYGVYYVDHINRKTQYENPVVEARRQKLLEQQQQPQPPEEWIEEHSAAAAPLANYAPNHLETYRDPQVPPALPPGPAGIKRGKPFFTRNPAELKGTFINTKLKKSHRGFGFTVVGGDEPDEFLQIKSLVLDGPAAVDGKMETGDVIVSVNDTIVLGYTHAQVVKIFQSIPIGSMVDLALCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETFDTPPNQAGPLNSMREPRPLSPSAEVASNGSHGYSSDVVTLASSIATQPELITIHMEKGDKGFGFTIADSLTGGGQRVKQIVDYPRCRGLKEGDILMEVNKRNVQNMSHNQVVDLLSKCPRGSEVTMLVQRGVVPAKRSPKLVHQLERKDSQNSSQHSVCSHRSTHTDSPSHPPSAMPSEAVVPTPAAPTQPLPVLPPQDPADGMLTLQKKPDPFKIWAQSRSMYESRLPDCQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTPGYGGSKGDGDVPPSPASSHHSSTQAPSLTEGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGSAVVPASLQPYDVEIQRGENEGFGFVIVSSVSRPDAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVSLRIIPGDESSNASLLTNAEKIATITTTHTPHSTETRNNSKSKGAPPPPPSQTQTQEAEFYSVDLERDSKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKSMKHSRAIELIKNGGRRARLVLKRGDGSVPEYDGSSDGHPPTPRAQNTPEVRTLPPSSRYHTSLESSYPPDLHKSSRLEEAACGRGMDKNRDRAGSDQMEFQGRQFNPHHHYTWNNNHSQSTPRQQSPDSNSTSSGNKKTRGRKVKKSESESGISKLLKTLRKDSSGKKAAAAEKLRGRELSSSGSTLDEKYLRQRRGSLDRSPTRKRTSSPDRRRAKSMDRRAERGHRDRTPEREHDDGGFLSVTPERRFHERRLLKDSQIAGRVSEASTPERANNSMESLSRSRGRTDDKATKNGNLLRDSSSERREDRHRMNGTMERRYRAERDSSPDSNYSRDELNYAEAPRLKDYYSMMKNNAAHNNATYNNAGHNNNTVGRSSNQVPEPKRRLYKESPKDLSI

Target ORF information:

RefSeq Version	XM_017409524.2
Organism	Kryptolebias marmoratus(mangrove rivulus)
Definition	Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017409524.2

ORF Insert Sequence:

ATGTCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACGA AAGTGAAGGA GTGCGCCGTG 
GTTCGGGACC CCAGCGGGGA GCTGAACTTG GAGCTGCGCG GCGGGGCGCA GGACGGAGAG 
TTCGCCTACA TCGGACACAT CAGGGAGGAT GCTGTGCTCC ACCAGGACGG GAAACTGTCT 
GAGGGAGAGC TGCTGCTGGA GGTGGAGGGG CTGTCCGTGT CTGGATTACC TCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAAGGT CCCGTCAGGT TCAAAACTGT CCGCCAAGGT 
CACAAGCTCA ACAAGGACCT AAAGCACTTC CTGAGCTTGA GGTTTCAGAA GTCATCACCT 
GACCACGAGT TGCAGAAGAC TATCCGAGCC AATCTATATC GTCATGCCGT GCCTTGTACA 
ACCCGCGCCC CTCGAGACGG AGAGGTGCCA GGGGTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTTGAGGA GAGTGGCACA CTACTGGAAA TAGGAACGTA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC GCCGAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
ACGGGCTCCC ATCAGTCCAC CCCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAACGTC 
CGCGTAGTGC CTGCTGACGA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGTAACC CTAACGACCA GGACGAGAGC CGTACCGGCA TCCTTCGGCC ATATCCTGTG 
CGTGATAATG CTCTGGCCTG TGGTCTGAAC AATGCCACCA CCTCCACTGT TGAAAGTGGG 
CAGCAGACGC TCGCAGAACC ACAGGTCTCT CCAGATGACC CTCTGGGACC GCTGCCTGAA 
AACTGGGAGA TGGCCTACAC TGAGAACGGG GAGCTGTACT TCATAGACCA CAACACAAAG 
ACCACATCAT GGCTGGATCC GCGGTGCCGA GACAAAGCCT CCAGGCCTTT GGAAGAGTGT 
GATGATGATG AAGGGATACA TACGGAAGAC CTGGAAAGTG ATCTAGAGCT GCCCCCAGGA 
TGGGAAAGAA TAGATGACCC CGTGTACGGA GTGTACTATG TAGATCATAT AAACAGGAAG 
ACCCAATATG AGAACCCAGT GGTGGAGGCG CGGCGCCAAA AACTCTTGGA GCAGCAGCAA 
CAGCCGCAGC CTCCAGAAGA GTGGATAGAG GAGCACTCAG CTGCAGCAGC TCCTTTGGCA 
AACTATGCTC CAAACCACCT GGAGACCTAC AGGGACCCAC AAGTCCCTCC AGCTCTACCT 
CCAGGCCCCG CTGGCATCAA AAGAGGGAAA CCTTTCTTTA CGAGAAACCC AGCAGAGCTG 
AAGGGAACCT TCATCAACAC CAAGCTGAAG AAAAGTCACC GTGGATTCGG GTTCACCGTG 
GTGGGAGGTG ACGAGCCCGA CGAGTTCCTG CAGATCAAGA GTCTGGTGCT GGATGGGCCT 
GCGGCTGTTG ATGGCAAGAT GGAGACAGGT GATGTGATTG TCAGCGTGAA TGACACTATT 
GTGCTGGGCT ACACTCATGC TCAGGTGGTG AAGATCTTCC AGTCCATCCC TATCGGCTCC 
ATGGTGGACC TGGCCTTATG CCGTGGCTAC CCACTGCCCT TTGACCCAGA CGACCCAAAC 
ACCAGCCTGG TGACCTCTGT GGCCATCTTG GACAAGGAAC CCATTATTGT CAATGGGCAA 
GAGACGTTTG ACACGCCGCC TAACCAGGCT GGACCCCTTA ACAGCATGCG TGAGCCACGG 
CCTCTCAGCC CCTCAGCTGA GGTGGCGTCC AACGGCTCCC ATGGTTACTC CAGCGACGTG 
GTCACGTTGG CCTCGTCCAT AGCCACTCAG CCCGAGCTCA TCACCATCCA CATGGAGAAG 
GGGGACAAAG GTTTTGGCTT CACCATCGCT GACAGCCTAA CAGGCGGAGG CCAGAGGGTA 
AAGCAGATAG TGGACTATCC ACGGTGCCGC GGCCTGAAAG AGGGGGACAT TCTAATGGAG 
GTCAACAAGA GAAATGTCCA GAATATGAGT CACAATCAGG TGGTGGATCT GCTGAGCAAA 
TGTCCAAGAG GCAGTGAGGT CACCATGTTG GTGCAAAGAG GAGTGGTGCC AGCCAAGAGG 
AGCCCAAAGC TGGTGCACCA ACTGGAGAGA AAAGACAGTC AGAACAGCTC CCAGCACAGT 
GTGTGCAGCC ATCGCAGCAC CCACACCGAT TCTCCCAGCC ACCCACCCTC TGCCATGCCC 
AGTGAGGCAG TGGTCCCCAC TCCCGCTGCC CCCACTCAGC CTCTGCCAGT CTTGCCCCCC 
CAGGACCCCG CAGATGGCAT GCTCACCCTC CAGAAGAAGC CAGACCCTTT TAAGATCTGG 
GCACAGTCCA GGAGCATGTA TGAGAGTCGA CTACCAGATT GCCAGGAGCA AGACATTTTC 
CTTTGGCGGA AAGATACAGG TTTTGGGTTT CGCATCTTGG GAGGAAATGA GCCTGGGGAG 
CCTATATACA TAGGGCACAT AGTGAAGTAC GGGGCCGCAG ATGAAGATGG CCGCCTGCGA 
TCGGGTGACG AGCTCATCTG TGTGGACGGC ACGGCTGTAG TGGGCAAATC TCACCAGCTG 
GTGGTGCAGC TGATGCAGCA GGCAGCCAAG CAGGGCCACG TCAACCTCAC TGTCAGACGC 
AAGACCCCCG GATACGGAGG GTCAAAAGGA GATGGTGACG TTCCTCCATC CCCAGCCTCC 
TCCCACCACA GCAGCACCCA GGCACCCAGT CTGACGGAGG GAAAGCGCAC CCCTCAGGGC 
AGCCAGAACT CACTCAACAC TGTCAGCTCC GGCAGCGGAT CCACCAGCGG CATAGGCAGC 
GGCGGTGGAG GAGGCAGTGG GAGCGCGGTG GTGCCTGCCT CCCTGCAGCC TTACGACGTG 
GAGATCCAGC GAGGAGAGAA CGAGGGATTT GGTTTTGTCA TCGTGTCGTC CGTTTCCAGG 
CCTGATGCTG GCACTACTTT CGCTGGAAAT GCTTGTGTGG CAATGCCCCA CAAAATAGGA 
CGCATCATCG AGGGCAGCCC GGCGGACCGC TGCGGGAAGC TGAAGGTCGG GGACAGAATA 
CTGGCTGTTA ACGGCTGCTC CATCACCAAC AAGTCTCACT CAGACATCGT CAACCTGATT 
AAGGAAGCCG GGAACACCGT CTCACTCAGG ATCATCCCAG GTGATGAATC TTCCAATGCT 
TCTCTGCTGA CTAATGCTGA GAAGATAGCT ACCATCACCA CGACACACAC ACCTCATTCC 
ACGGAGACCA GAAATAATTC AAAGTCAAAA GGAGCTCCTC CTCCACCCCC TTCACAGACC 
CAAACACAGG AAGCAGAGTT TTATTCTGTG GACCTCGAGA GGGACAGTAA AGGCTTCGGC 
TTCAGCTTAA GAGGAGGACG GGAGTACAAC ATGGACCTGT ACGTGTTGAG ACTAGCAGAG 
GATGGGGCTG CTGTCAGAAA TGGCAAGATG AGGGTGGGAG ACGAGATCCT GGAGATTAAT 
GGTGAGAGCA CAAAGAGCAT GAAGCATTCA CGAGCCATTG AACTGATCAA GAACGGCGGT 
CGGAGGGCAC GACTTGTCCT AAAAAGGGGC GATGGATCTG TACCTGAATA TGACGGCAGC 
AGTGACGGGC ACCCTCCCAC ACCCCGGGCA CAAAACACTC CGGAAGTGAG AACGTTGCCA 
CCCAGCAGCC GATATCACAC CTCATTGGAG TCCAGTTATC CACCAGACCT TCACAAATCA 
TCACGCCTGG AGGAGGCTGC TTGTGGCCGA GGTATGGACA AGAACAGGGA CAGGGCTGGT 
TCAGATCAGA TGGAATTCCA AGGCCGACAA TTTAACCCCC ATCACCATTA CACTTGGAAT 
AATAATCACA GTCAAAGTAC CCCCAGACAG CAGTCCCCTG ACAGTAATAG CACTAGCAGT 
GGCAACAAGA AGACCAGAGG CCGTAAAGTC AAGAAGTCTG AATCGGAGAG TGGCATCTCT 
AAACTTCTAA AGACTCTGAG GAAGGACAGC TCGGGAAAAA AGGCTGCAGC CGCCGAAAAA 
CTAAGGGGCC GAGAGCTTTC AAGCAGCGGT TCTACTTTGG ATGAGAAGTA TCTTCGGCAG 
CGTCGTGGGT CCCTCGACCG CTCACCAACC AGAAAGCGCA CCTCGTCTCC AGATCGTCGA 
CGGGCGAAAT CCATGGACCG CAGGGCAGAG CGAGGACATC GTGACCGCAC ACCTGAGAGG 
GAACACGATG ATGGAGGGTT TCTCTCAGTC ACCCCTGAAC GGAGGTTCCA CGAGCGAAGG 
CTCCTCAAGG ACTCACAGAT AGCTGGACGA GTCAGTGAGG CTTCAACCCC CGAGCGCGCC 
AACAACAGCA TGGAATCCTT GTCTCGTTCC AGGGGCCGCA CGGACGATAA AGCCACAAAG 
AACGGCAACC TTCTGAGAGA CTCTTCTTCA GAGAGGAGAG AGGACCGACA TCGCATGAAT 
GGGACAATGG AGAGGCGATA CAGAGCGGAG CGGGACTCTT CCCCTGACAG CAACTACAGC 
CGGGACGAGC TGAACTATGC AGAAGCACCT AGACTGAAGG ACTACTACAG CATGATGAAG 
AACAACGCTG CACACAACAA TGCTACCTAC AACAACGCAG GCCATAACAA TAACACAGTA 
GGCCGCAGCT CAAACCAGGT CCCTGAGCCC AAGAGGAGGC TGTACAAAGA AAGTCCTAAA 
GACCTCAGCA TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108232031 ( XM_017409524.1 ) cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus) -> XP_017265013.1 Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OKr06774
Clone ID Related Accession (Same CDS sequence)	XM_017409524.2 , XM_017409524.1
Accession Version	XM_017409524.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-27
Organism	Kryptolebias marmoratus(mangrove rivulus)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016094253.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Kryptolebias marmoratus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACGA AAGTGAAGGA GTGCGCCGTG 
GTTCGGGACC CCAGCGGGGA GCTGAACTTG GAGCTGCGCG GCGGGGCGCA GGACGGAGAG 
TTCGCCTACA TCGGACACAT CAGGGAGGAT GCTGTGCTCC ACCAGGACGG GAAACTGTCT 
GAGGGAGAGC TGCTGCTGGA GGTGGAGGGG CTGTCCGTGT CTGGATTACC TCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAAGGT CCCGTCAGGT TCAAAACTGT CCGCCAAGGT 
CACAAGCTCA ACAAGGACCT AAAGCACTTC CTGAGCTTGA GGTTTCAGAA GTCATCACCT 
GACCACGAGT TGCAGAAGAC TATCCGAGCC AATCTATATC GTCATGCCGT GCCTTGTACA 
ACCCGCGCCC CTCGAGACGG AGAGGTGCCA GGGGTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTTGAGGA GAGTGGCACA CTACTGGAAA TAGGAACGTA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC GCCGAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
ACGGGCTCCC ATCAGTCCAC CCCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAACGTC 
CGCGTAGTGC CTGCTGACGA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGTAACC CTAACGACCA GGACGAGAGC CGTACCGGCA TCCTTCGGCC ATATCCTGTG 
CGTGATAATG CTCTGGCCTG TGGTCTGAAC AATGCCACCA CCTCCACTGT TGAAAGTGGG 
CAGCAGACGC TCGCAGAACC ACAGGTCTCT CCAGATGACC CTCTGGGACC GCTGCCTGAA 
AACTGGGAGA TGGCCTACAC TGAGAACGGG GAGCTGTACT TCATAGACCA CAACACAAAG 
ACCACATCAT GGCTGGATCC GCGGTGCCGA GACAAAGCCT CCAGGCCTTT GGAAGAGTGT 
GATGATGATG AAGGGATACA TACGGAAGAC CTGGAAAGTG ATCTAGAGCT GCCCCCAGGA 
TGGGAAAGAA TAGATGACCC CGTGTACGGA GTGTACTATG TAGATCATAT AAACAGGAAG 
ACCCAATATG AGAACCCAGT GGTGGAGGCG CGGCGCCAAA AACTCTTGGA GCAGCAGCAA 
CAGCCGCAGC CTCCAGAAGA GTGGATAGAG GAGCACTCAG CTGCAGCAGC TCCTTTGGCA 
AACTATGCTC CAAACCACCT GGAGACCTAC AGGGACCCAC AAGTCCCTCC AGCTCTACCT 
CCAGGCCCCG CTGGCATCAA AAGAGGGAAA CCTTTCTTTA CGAGAAACCC AGCAGAGCTG 
AAGGGAACCT TCATCAACAC CAAGCTGAAG AAAAGTCACC GTGGATTCGG GTTCACCGTG 
GTGGGAGGTG ACGAGCCCGA CGAGTTCCTG CAGATCAAGA GTCTGGTGCT GGATGGGCCT 
GCGGCTGTTG ATGGCAAGAT GGAGACAGGT GATGTGATTG TCAGCGTGAA TGACACTATT 
GTGCTGGGCT ACACTCATGC TCAGGTGGTG AAGATCTTCC AGTCCATCCC TATCGGCTCC 
ATGGTGGACC TGGCCTTATG CCGTGGCTAC CCACTGCCCT TTGACCCAGA CGACCCAAAC 
ACCAGCCTGG TGACCTCTGT GGCCATCTTG GACAAGGAAC CCATTATTGT CAATGGGCAA 
GAGACGTTTG ACACGCCGCC TAACCAGGCT GGACCCCTTA ACAGCATGCG TGAGCCACGG 
CCTCTCAGCC CCTCAGCTGA GGTGGCGTCC AACGGCTCCC ATGGTTACTC CAGCGACGTG 
GTCACGTTGG CCTCGTCCAT AGCCACTCAG CCCGAGCTCA TCACCATCCA CATGGAGAAG 
GGGGACAAAG GTTTTGGCTT CACCATCGCT GACAGCCTAA CAGGCGGAGG CCAGAGGGTA 
AAGCAGATAG TGGACTATCC ACGGTGCCGC GGCCTGAAAG AGGGGGACAT TCTAATGGAG 
GTCAACAAGA GAAATGTCCA GAATATGAGT CACAATCAGG TGGTGGATCT GCTGAGCAAA 
TGTCCAAGAG GCAGTGAGGT CACCATGTTG GTGCAAAGAG GAGTGGTGCC AGCCAAGAGG 
AGCCCAAAGC TGGTGCACCA ACTGGAGAGA AAAGACAGTC AGAACAGCTC CCAGCACAGT 
GTGTGCAGCC ATCGCAGCAC CCACACCGAT TCTCCCAGCC ACCCACCCTC TGCCATGCCC 
AGTGAGGCAG TGGTCCCCAC TCCCGCTGCC CCCACTCAGC CTCTGCCAGT CTTGCCCCCC 
CAGGACCCCG CAGATGGCAT GCTCACCCTC CAGAAGAAGC CAGACCCTTT TAAGATCTGG 
GCACAGTCCA GGAGCATGTA TGAGAGTCGA CTACCAGATT GCCAGGAGCA AGACATTTTC 
CTTTGGCGGA AAGATACAGG TTTTGGGTTT CGCATCTTGG GAGGAAATGA GCCTGGGGAG 
CCTATATACA TAGGGCACAT AGTGAAGTAC GGGGCCGCAG ATGAAGATGG CCGCCTGCGA 
TCGGGTGACG AGCTCATCTG TGTGGACGGC ACGGCTGTAG TGGGCAAATC TCACCAGCTG 
GTGGTGCAGC TGATGCAGCA GGCAGCCAAG CAGGGCCACG TCAACCTCAC TGTCAGACGC 
AAGACCCCCG GATACGGAGG GTCAAAAGGA GATGGTGACG TTCCTCCATC CCCAGCCTCC 
TCCCACCACA GCAGCACCCA GGCACCCAGT CTGACGGAGG GAAAGCGCAC CCCTCAGGGC 
AGCCAGAACT CACTCAACAC TGTCAGCTCC GGCAGCGGAT CCACCAGCGG CATAGGCAGC 
GGCGGTGGAG GAGGCAGTGG GAGCGCGGTG GTGCCTGCCT CCCTGCAGCC TTACGACGTG 
GAGATCCAGC GAGGAGAGAA CGAGGGATTT GGTTTTGTCA TCGTGTCGTC CGTTTCCAGG 
CCTGATGCTG GCACTACTTT CGCTGGAAAT GCTTGTGTGG CAATGCCCCA CAAAATAGGA 
CGCATCATCG AGGGCAGCCC GGCGGACCGC TGCGGGAAGC TGAAGGTCGG GGACAGAATA 
CTGGCTGTTA ACGGCTGCTC CATCACCAAC AAGTCTCACT CAGACATCGT CAACCTGATT 
AAGGAAGCCG GGAACACCGT CTCACTCAGG ATCATCCCAG GTGATGAATC TTCCAATGCT 
TCTCTGCTGA CTAATGCTGA GAAGATAGCT ACCATCACCA CGACACACAC ACCTCATTCC 
ACGGAGACCA GAAATAATTC AAAGTCAAAA GGAGCTCCTC CTCCACCCCC TTCACAGACC 
CAAACACAGG AAGCAGAGTT TTATTCTGTG GACCTCGAGA GGGACAGTAA AGGCTTCGGC 
TTCAGCTTAA GAGGAGGACG GGAGTACAAC ATGGACCTGT ACGTGTTGAG ACTAGCAGAG 
GATGGGGCTG CTGTCAGAAA TGGCAAGATG AGGGTGGGAG ACGAGATCCT GGAGATTAAT 
GGTGAGAGCA CAAAGAGCAT GAAGCATTCA CGAGCCATTG AACTGATCAA GAACGGCGGT 
CGGAGGGCAC GACTTGTCCT AAAAAGGGGC GATGGATCTG TACCTGAATA TGACGGCAGC 
AGTGACGGGC ACCCTCCCAC ACCCCGGGCA CAAAACACTC CGGAAGTGAG AACGTTGCCA 
CCCAGCAGCC GATATCACAC CTCATTGGAG TCCAGTTATC CACCAGACCT TCACAAATCA 
TCACGCCTGG AGGAGGCTGC TTGTGGCCGA GGTATGGACA AGAACAGGGA CAGGGCTGGT 
TCAGATCAGA TGGAATTCCA AGGCCGACAA TTTAACCCCC ATCACCATTA CACTTGGAAT 
AATAATCACA GTCAAAGTAC CCCCAGACAG CAGTCCCCTG ACAGTAATAG CACTAGCAGT 
GGCAACAAGA AGACCAGAGG CCGTAAAGTC AAGAAGTCTG AATCGGAGAG TGGCATCTCT 
AAACTTCTAA AGACTCTGAG GAAGGACAGC TCGGGAAAAA AGGCTGCAGC CGCCGAAAAA 
CTAAGGGGCC GAGAGCTTTC AAGCAGCGGT TCTACTTTGG ATGAGAAGTA TCTTCGGCAG 
CGTCGTGGGT CCCTCGACCG CTCACCAACC AGAAAGCGCA CCTCGTCTCC AGATCGTCGA 
CGGGCGAAAT CCATGGACCG CAGGGCAGAG CGAGGACATC GTGACCGCAC ACCTGAGAGG 
GAACACGATG ATGGAGGGTT TCTCTCAGTC ACCCCTGAAC GGAGGTTCCA CGAGCGAAGG 
CTCCTCAAGG ACTCACAGAT AGCTGGACGA GTCAGTGAGG CTTCAACCCC CGAGCGCGCC 
AACAACAGCA TGGAATCCTT GTCTCGTTCC AGGGGCCGCA CGGACGATAA AGCCACAAAG 
AACGGCAACC TTCTGAGAGA CTCTTCTTCA GAGAGGAGAG AGGACCGACA TCGCATGAAT 
GGGACAATGG AGAGGCGATA CAGAGCGGAG CGGGACTCTT CCCCTGACAG CAACTACAGC 
CGGGACGAGC TGAACTATGC AGAAGCACCT AGACTGAAGG ACTACTACAG CATGATGAAG 
AACAACGCTG CACACAACAA TGCTACCTAC AACAACGCAG GCCATAACAA TAACACAGTA 
GGCCGCAGCT CAAACCAGGT CCCTGAGCCC AAGAGGAGGC TGTACAAAGA AAGTCCTAAA 
GACCTCAGCA TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017265013.1
CDS	773..5527
Translation	MSKVIQKKNHWITKVKECAVVRDPSGELNLELRGGAQDGEFAYIGHIREDAVLHQDGKLSEGELLLEVEGLSVSGLPLYDIYTVINCCKGPVRFKTVRQGHKLNKDLKHFLSLRFQKSSPDHELQKTIRANLYRHAVPCTTRAPRDGEVPGVDYNFLSVEDFLQLEESGTLLEIGTYEGNYYGTPKPPRQPIIGTVTLSDTGSHQSTPKRTKSYNDMQNVRVVPADDDDDDQATEMNNSFTGNPNDQDESRTGILRPYPVRDNALACGLNNATTSTVESGQQTLAEPQVSPDDPLGPLPENWEMAYTENGELYFIDHNTKTTSWLDPRCRDKASRPLEECDDDEGIHTEDLESDLELPPGWERIDDPVYGVYYVDHINRKTQYENPVVEARRQKLLEQQQQPQPPEEWIEEHSAAAAPLANYAPNHLETYRDPQVPPALPPGPAGIKRGKPFFTRNPAELKGTFINTKLKKSHRGFGFTVVGGDEPDEFLQIKSLVLDGPAAVDGKMETGDVIVSVNDTIVLGYTHAQVVKIFQSIPIGSMVDLALCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETFDTPPNQAGPLNSMREPRPLSPSAEVASNGSHGYSSDVVTLASSIATQPELITIHMEKGDKGFGFTIADSLTGGGQRVKQIVDYPRCRGLKEGDILMEVNKRNVQNMSHNQVVDLLSKCPRGSEVTMLVQRGVVPAKRSPKLVHQLERKDSQNSSQHSVCSHRSTHTDSPSHPPSAMPSEAVVPTPAAPTQPLPVLPPQDPADGMLTLQKKPDPFKIWAQSRSMYESRLPDCQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTPGYGGSKGDGDVPPSPASSHHSSTQAPSLTEGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGSAVVPASLQPYDVEIQRGENEGFGFVIVSSVSRPDAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVSLRIIPGDESSNASLLTNAEKIATITTTHTPHSTETRNNSKSKGAPPPPPSQTQTQEAEFYSVDLERDSKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKSMKHSRAIELIKNGGRRARLVLKRGDGSVPEYDGSSDGHPPTPRAQNTPEVRTLPPSSRYHTSLESSYPPDLHKSSRLEEAACGRGMDKNRDRAGSDQMEFQGRQFNPHHHYTWNNNHSQSTPRQQSPDSNSTSSGNKKTRGRKVKKSESESGISKLLKTLRKDSSGKKAAAAEKLRGRELSSSGSTLDEKYLRQRRGSLDRSPTRKRTSSPDRRRAKSMDRRAERGHRDRTPEREHDDGGFLSVTPERRFHERRLLKDSQIAGRVSEASTPERANNSMESLSRSRGRTDDKATKNGNLLRDSSSERREDRHRMNGTMERRYRAERDSSPDSNYSRDELNYAEAPRLKDYYSMMKNNAAHNNATYNNAGHNNNTVGRSSNQVPEPKRRLYKESPKDLSI

Target ORF information:

RefSeq Version	XM_017409524.1
Organism	Kryptolebias marmoratus(mangrove rivulus)
Definition	Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017409524.1

ORF Insert Sequence:

ATGTCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACGA AAGTGAAGGA GTGCGCCGTG 
GTTCGGGACC CCAGCGGGGA GCTGAACTTG GAGCTGCGCG GCGGGGCGCA GGACGGAGAG 
TTCGCCTACA TCGGACACAT CAGGGAGGAT GCTGTGCTCC ACCAGGACGG GAAACTGTCT 
GAGGGAGAGC TGCTGCTGGA GGTGGAGGGG CTGTCCGTGT CTGGATTACC TCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAAGGT CCCGTCAGGT TCAAAACTGT CCGCCAAGGT 
CACAAGCTCA ACAAGGACCT AAAGCACTTC CTGAGCTTGA GGTTTCAGAA GTCATCACCT 
GACCACGAGT TGCAGAAGAC TATCCGAGCC AATCTATATC GTCATGCCGT GCCTTGTACA 
ACCCGCGCCC CTCGAGACGG AGAGGTGCCA GGGGTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTTGAGGA GAGTGGCACA CTACTGGAAA TAGGAACGTA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC GCCGAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
ACGGGCTCCC ATCAGTCCAC CCCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAACGTC 
CGCGTAGTGC CTGCTGACGA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGTAACC CTAACGACCA GGACGAGAGC CGTACCGGCA TCCTTCGGCC ATATCCTGTG 
CGTGATAATG CTCTGGCCTG TGGTCTGAAC AATGCCACCA CCTCCACTGT TGAAAGTGGG 
CAGCAGACGC TCGCAGAACC ACAGGTCTCT CCAGATGACC CTCTGGGACC GCTGCCTGAA 
AACTGGGAGA TGGCCTACAC TGAGAACGGG GAGCTGTACT TCATAGACCA CAACACAAAG 
ACCACATCAT GGCTGGATCC GCGGTGCCGA GACAAAGCCT CCAGGCCTTT GGAAGAGTGT 
GATGATGATG AAGGGATACA TACGGAAGAC CTGGAAAGTG ATCTAGAGCT GCCCCCAGGA 
TGGGAAAGAA TAGATGACCC CGTGTACGGA GTGTACTATG TAGATCATAT AAACAGGAAG 
ACCCAATATG AGAACCCAGT GGTGGAGGCG CGGCGCCAAA AACTCTTGGA GCAGCAGCAA 
CAGCCGCAGC CTCCAGAAGA GTGGATAGAG GAGCACTCAG CTGCAGCAGC TCCTTTGGCA 
AACTATGCTC CAAACCACCT GGAGACCTAC AGGGACCCAC AAGTCCCTCC AGCTCTACCT 
CCAGGCCCCG CTGGCATCAA AAGAGGGAAA CCTTTCTTTA CGAGAAACCC AGCAGAGCTG 
AAGGGAACCT TCATCAACAC CAAGCTGAAG AAAAGTCACC GTGGATTCGG GTTCACCGTG 
GTGGGAGGTG ACGAGCCCGA CGAGTTCCTG CAGATCAAGA GTCTGGTGCT GGATGGGCCT 
GCGGCTGTTG ATGGCAAGAT GGAGACAGGT GATGTGATTG TCAGCGTGAA TGACACTATT 
GTGCTGGGCT ACACTCATGC TCAGGTGGTG AAGATCTTCC AGTCCATCCC TATCGGCTCC 
ATGGTGGACC TGGCCTTATG CCGTGGCTAC CCACTGCCCT TTGACCCAGA CGACCCAAAC 
ACCAGCCTGG TGACCTCTGT GGCCATCTTG GACAAGGAAC CCATTATTGT CAATGGGCAA 
GAGACGTTTG ACACGCCGCC TAACCAGGCT GGACCCCTTA ACAGCATGCG TGAGCCACGG 
CCTCTCAGCC CCTCAGCTGA GGTGGCGTCC AACGGCTCCC ATGGTTACTC CAGCGACGTG 
GTCACGTTGG CCTCGTCCAT AGCCACTCAG CCCGAGCTCA TCACCATCCA CATGGAGAAG 
GGGGACAAAG GTTTTGGCTT CACCATCGCT GACAGCCTAA CAGGCGGAGG CCAGAGGGTA 
AAGCAGATAG TGGACTATCC ACGGTGCCGC GGCCTGAAAG AGGGGGACAT TCTAATGGAG 
GTCAACAAGA GAAATGTCCA GAATATGAGT CACAATCAGG TGGTGGATCT GCTGAGCAAA 
TGTCCAAGAG GCAGTGAGGT CACCATGTTG GTGCAAAGAG GAGTGGTGCC AGCCAAGAGG 
AGCCCAAAGC TGGTGCACCA ACTGGAGAGA AAAGACAGTC AGAACAGCTC CCAGCACAGT 
GTGTGCAGCC ATCGCAGCAC CCACACCGAT TCTCCCAGCC ACCCACCCTC TGCCATGCCC 
AGTGAGGCAG TGGTCCCCAC TCCCGCTGCC CCCACTCAGC CTCTGCCAGT CTTGCCCCCC 
CAGGACCCCG CAGATGGCAT GCTCACCCTC CAGAAGAAGC CAGACCCTTT TAAGATCTGG 
GCACAGTCCA GGAGCATGTA TGAGAGTCGA CTACCAGATT GCCAGGAGCA AGACATTTTC 
CTTTGGCGGA AAGATACAGG TTTTGGGTTT CGCATCTTGG GAGGAAATGA GCCTGGGGAG 
CCTATATACA TAGGGCACAT AGTGAAGTAC GGGGCCGCAG ATGAAGATGG CCGCCTGCGA 
TCGGGTGACG AGCTCATCTG TGTGGACGGC ACGGCTGTAG TGGGCAAATC TCACCAGCTG 
GTGGTGCAGC TGATGCAGCA GGCAGCCAAG CAGGGCCACG TCAACCTCAC TGTCAGACGC 
AAGACCCCCG GATACGGAGG GTCAAAAGGA GATGGTGACG TTCCTCCATC CCCAGCCTCC 
TCCCACCACA GCAGCACCCA GGCACCCAGT CTGACGGAGG GAAAGCGCAC CCCTCAGGGC 
AGCCAGAACT CACTCAACAC TGTCAGCTCC GGCAGCGGAT CCACCAGCGG CATAGGCAGC 
GGCGGTGGAG GAGGCAGTGG GAGCGCGGTG GTGCCTGCCT CCCTGCAGCC TTACGACGTG 
GAGATCCAGC GAGGAGAGAA CGAGGGATTT GGTTTTGTCA TCGTGTCGTC CGTTTCCAGG 
CCTGATGCTG GCACTACTTT CGCTGGAAAT GCTTGTGTGG CAATGCCCCA CAAAATAGGA 
CGCATCATCG AGGGCAGCCC GGCGGACCGC TGCGGGAAGC TGAAGGTCGG GGACAGAATA 
CTGGCTGTTA ACGGCTGCTC CATCACCAAC AAGTCTCACT CAGACATCGT CAACCTGATT 
AAGGAAGCCG GGAACACCGT CTCACTCAGG ATCATCCCAG GTGATGAATC TTCCAATGCT 
TCTCTGCTGA CTAATGCTGA GAAGATAGCT ACCATCACCA CGACACACAC ACCTCATTCC 
ACGGAGACCA GAAATAATTC AAAGTCAAAA GGAGCTCCTC CTCCACCCCC TTCACAGACC 
CAAACACAGG AAGCAGAGTT TTATTCTGTG GACCTCGAGA GGGACAGTAA AGGCTTCGGC 
TTCAGCTTAA GAGGAGGACG GGAGTACAAC ATGGACCTGT ACGTGTTGAG ACTAGCAGAG 
GATGGGGCTG CTGTCAGAAA TGGCAAGATG AGGGTGGGAG ACGAGATCCT GGAGATTAAT 
GGTGAGAGCA CAAAGAGCAT GAAGCATTCA CGAGCCATTG AACTGATCAA GAACGGCGGT 
CGGAGGGCAC GACTTGTCCT AAAAAGGGGC GATGGATCTG TACCTGAATA TGACGGCAGC 
AGTGACGGGC ACCCTCCCAC ACCCCGGGCA CAAAACACTC CGGAAGTGAG AACGTTGCCA 
CCCAGCAGCC GATATCACAC CTCATTGGAG TCCAGTTATC CACCAGACCT TCACAAATCA 
TCACGCCTGG AGGAGGCTGC TTGTGGCCGA GGTATGGACA AGAACAGGGA CAGGGCTGGT 
TCAGATCAGA TGGAATTCCA AGGCCGACAA TTTAACCCCC ATCACCATTA CACTTGGAAT 
AATAATCACA GTCAAAGTAC CCCCAGACAG CAGTCCCCTG ACAGTAATAG CACTAGCAGT 
GGCAACAAGA AGACCAGAGG CCGTAAAGTC AAGAAGTCTG AATCGGAGAG TGGCATCTCT 
AAACTTCTAA AGACTCTGAG GAAGGACAGC TCGGGAAAAA AGGCTGCAGC CGCCGAAAAA 
CTAAGGGGCC GAGAGCTTTC AAGCAGCGGT TCTACTTTGG ATGAGAAGTA TCTTCGGCAG 
CGTCGTGGGT CCCTCGACCG CTCACCAACC AGAAAGCGCA CCTCGTCTCC AGATCGTCGA 
CGGGCGAAAT CCATGGACCG CAGGGCAGAG CGAGGACATC GTGACCGCAC ACCTGAGAGG 
GAACACGATG ATGGAGGGTT TCTCTCAGTC ACCCCTGAAC GGAGGTTCCA CGAGCGAAGG 
CTCCTCAAGG ACTCACAGAT AGCTGGACGA GTCAGTGAGG CTTCAACCCC CGAGCGCGCC 
AACAACAGCA TGGAATCCTT GTCTCGTTCC AGGGGCCGCA CGGACGATAA AGCCACAAAG 
AACGGCAACC TTCTGAGAGA CTCTTCTTCA GAGAGGAGAG AGGACCGACA TCGCATGAAT 
GGGACAATGG AGAGGCGATA CAGAGCGGAG CGGGACTCTT CCCCTGACAG CAACTACAGC 
CGGGACGAGC TGAACTATGC AGAAGCACCT AGACTGAAGG ACTACTACAG CATGATGAAG 
AACAACGCTG CACACAACAA TGCTACCTAC AACAACGCAG GCCATAACAA TAACACAGTA 
GGCCGCAGCT CAAACCAGGT CCCTGAGCCC AAGAGGAGGC TGTACAAAGA AAGTCCTAAA 
GACCTCAGCA TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108232031 ( XM_025004507.1 ) cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus) -> XP_024860275.1 Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X4, mRNA.

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CloneID	OKr34860
Clone ID Related Accession (Same CDS sequence)	XM_025004507.1
Accession Version	XM_025004507.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-03
Organism	Kryptolebias marmoratus(mangrove rivulus)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016094253.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Kryptolebias marmoratus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACGA AAGTGAAGGA GTGCGCCGTG 
GTTCGGGACC CCAGCGGGGA GCTGAACTTG GAGCTGCGCG GCGGGGCGCA GGACGGAGAG 
TTCGCCTACA TCGGACACAT CAGGGAGGAT GCTGTGCTCC ACCAGGACGG GAAACTGTCT 
GAGGGAGAGC TGCTGCTGGA GGTGGAGGGG CTGTCCGTGT CTGGATTACC TCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAAGGT CCCGTCAGGT TCAAAACTGT CCGCCAAGGT 
CACAAGCTCA ACAAGGACCT AAAGCACTTC CTGAGCTTGA GGTTTCAGAA GTCATCACCT 
GACCACGAGT TGCAGAAGAC TATCCGAGCC AATCTATATC GTCATGCCGT GCCTTGTACA 
ACCCGCGCCC CTCGAGACGG AGAGGTGCCA GGGGTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTTGAGGA GAGTGGCACA CTACTGGAAA TAGGAACGTA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC GCCGAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
ACGGGCTCCC ATCAGTCCAC CCCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAACGTC 
CGCGTAGTGC CTGCTGACGA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGAACCT TAGTGCGTAG CCTTTTCCCC TCTCCTTTGT TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGTAC CGGCATCCTT CGGCCATATC CTGTGCGTGA TAATGCTCTG 
GCCTGTGGTC TGAACAATGC CACCACCTCC ACTGTTGAAA GTGGGCAGCA GACGCTCGCA 
GAACCACAGG TCTCTCCAGA TGACCCTCTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACTGAGA ACGGGGAGCT GTACTTCATA GACACTGCAA AGCCTCACCA CCAGGACATG 
GCTGTGCATT TTCAGAGTGT CCACAACACA AAGACCACAT CATGGCTGGA TCCGCGGTGC 
CGAGACAAAG CCTCCAGGCC TTTGGAAGAG TGTGATGATG ATGAAGAAGG GATACATACG 
GAAGACCTGG AAAGTGATCT AGAGCTGCCC CCAGGATGGG AAAGAATAGA TGACCCCGTG 
TACGGAGTGT ACTATGTAGA TCATATAAAC AGGAAGACCC AATATGAGAA CCCAGTGGTG 
GAGGCGCGGC GCCAAAAACT CTTGGAGCAG CAGCAACAGC CGCAGCCTCC AGAAGGTGAG 
CGGTATATTC GAGAGTGGAT AGAGGAGCAC TCAGCTGCAG CAGCTCCTTT GGCAAACTAT 
GCTCCAAACC ACCTGGAGAC CTACAGGGAC CCACAAGTCC CTCCAGCTCT ACCTCCAGGC 
CCCGCTGGCA TCAAAAGAGG GAAACCTTTC TTTACGAGAA ACCCAGCAGA GCTGAAGGGA 
ACCTTCATCA ACACCAAGCT GAAGAAAAGT CACCGTGGAT TCGGGTTCAC CGTGGTGGGA 
GGTGACGAGC CCGACGAGTT CCTGCAGATC AAGAGTCTGG TGCTGGATGG GCCTGCGGCT 
GTTGATGGCA AGATGGAGAC AGGTGATGTG ATTGTCAGCG TGAATGACAC TATTGTGCTG 
GGCTACACTC ATGCTCAGGT GGTGAAGATC TTCCAGTCCA TCCCTATCGG CTCCATGGTG 
GACCTGGCCT TATGCCGTGG CTACCCACTG CCCTTTGACC CAGACGACCC AAACACCAGC 
CTGGTGACCT CTGTGGCCAT CTTGGACAAG GAACCCATTA TTGTCAATGG GCAAGAGACG 
TTTGACACGC CGCCTAACCA GGCTGGACCC CTTAACAGCA TGCGTGAGCC ACGGCCTCTC 
AGCCCCTCAG CTGAGGTGGC GTCCAACGGC TCCCATGGTT ACTCCAGCGA CGTGGTCACG 
TTGGCCTCGT CCATAGCCAC TCAGCCCGAG CTCATCACCA TCCACATGGA GAAGGGGGAC 
AAAGGTTTTG GCTTCACCAT CGCTGACAGC CTAACAGGCG GAGGCCAGAG GGTAAAGCAG 
ATAGTGGACT ATCCACGGTG CCGCGGCCTG AAAGAGGGGG ACATTCTAAT GGAGGTCAAC 
AAGAGAAATG TCCAGAATAT GAGTCACAAT CAGGTGGTGG ATCTGCTGAG CAAATGTCCA 
AGAGGCAGTG AGGTCACCAT GTTGGTGCAA AGAGGAGTGG TGCCAGCCAA GAGGAGCCCA 
AAGCTGGTGC ACCAACTGGA GAGAAAAGAC AGTCAGAACA GCTCCCAGCA CAGTGTGTGC 
AGCCATCGCA GCACCCACAC CGATTCTCCC AGCCACCCAC CCTCTGCCAT GCCCAGTGAG 
GCAGTGGTCC CCACTCCCGC TGCCCCCACT CAGCCTCTGC CAGTCTTGCC CCCCCAGGAC 
CCCGCAGATG GCATGCTCAC CCTCCAGAAG AAGCCAGACC CTTTTAAGAT CTGGGCACAG 
TCCAGGAGCA TGTATGAGAG TCGACTACCA GATTGCCAGG AGCAAGACAT TTTCCTTTGG 
CGGAAAGATA CAGGTTTTGG GTTTCGCATC TTGGGAGGAA ATGAGCCTGG GGAGCCTATA 
TACATAGGGC ACATAGTGAA GTACGGGGCC GCAGATGAAG ATGGCCGCCT GCGATCGGGT 
GACGAGCTCA TCTGTGTGGA CGGCACGGCT GTAGTGGGCA AATCTCACCA GCTGGTGGTG 
CAGCTGATGC AGCAGGCAGC CAAGCAGGGC CACGTCAACC TCACTGTCAG ACGCAAGACC 
CCCGGATACG GAGGGTCAAA AGGAGATGGT GACGTTCCTC CATCCCCAGC CTCCTCCCAC 
CACAGCAGCA CCCAGGCACC CAGTCTGACG GAGGGAAAGC GCACCCCTCA GGGCAGCCAG 
AACTCACTCA ACACTGTCAG CTCCGGCAGC GGATCCACCA GCGGCATAGG CAGCGGCGGT 
GGAGGAGGCA GTGGGAGCGC GGTGGTGCCT GCCTCCCTGC AGCCTTACGA CGTGGAGATC 
CAGCGAGGAG AGAACGAGGG ATTTGGTTTT GTCATCGTGT CGTCCGTTTC CAGGCCTGAT 
GCTGGCACTA CTTTCGGACG CATCATCGAG GGCAGCCCGG CGGACCGCTG CGGGAAGCTG 
AAGGTCGGGG ACAGAATACT GGCTGTTAAC GGCTGCTCCA TCACCAACAA GTCTCACTCA 
GACATCGTCA ACCTGATTAA GGAAGCCGGG AACACCGTCT CACTCAGGAT CATCCCAGGT 
GATGAATCTT CCAATGCTTC TCTGCTGACT AATGCTGAGA AGATAGCTAC CATCACCACG 
ACACACACAC CTCATTCCAC GGAGACCAGA AATAATTCAA AGTCAAAAGG AGCTCCTCCT 
CCACCCCCTT CACAGACCCA AACACAGGAA GCAGAGTTTT ATTCTGTGGA CCTCGAGAGG 
GACAGTAAAG GCTTCGGCTT CAGCTTAAGA GGAGGACGGG AGTACAACAT GGACCTGTAC 
GTGTTGAGAC TAGCAGAGGA TGGGGCTGCT GTCAGAAATG GCAAGATGAG GGTGGGAGAC 
GAGATCCTGG AGATTAATGG TGAGAGCACA AAGAGCATGA AGCATTCACG AGCCATTGAA 
CTGATCAAGA ACGGCGGTCG GAGGGCACGA CTTGTCCTAA AAAGGGGCGA TGGATCTGTA 
CCTGAATATG ACGGCAGCAG TGACGGGCAC CCTCCCACAC CCCGGGCACA AAACACTCCG 
GAAGTGAGAA CGTTGCCACC CAGCAGCCGA TATCACACCT CATTGGAGTC CAGTTATCCA 
CCAGACCTTC ACAAATCATC ACGCCTGGAG GAGGCTGCTT GTGGCCGAGG TATGGACAAG 
AACAGGGACA GGGCTGGTTC AGATCAGATG GAATTCCAAG GCCGACAATT TAACCCCCAT 
CACCATTACA CTTGGAATAA TAATCACAGT CAAAGTACCC CCAGACAGCA GTCCCCTGAC 
AGTAATAGCA CTAGCAGTGG CAACAAGAAG ACCAGAGGCC GTAAAGTCAA GAAGTCTGAA 
TCGGAGAGTG GCATCTCTAA ACTTCTAAAG ACTCTGAGGA AGGACAGCTC GGGAAAAAAG 
GCTGCAGCCG CCGAAAAACT AAGGGGCCGA GAGCTTTCAA GCAGCGGTTC TACTTTGGAT 
GAGAAGTATC TTCGGCAGCG TCGTGGGTCC CTCGACCGCT CACCAACCAG AAAGCGCACC 
TCGTCTCCAG ATCGTCGACG GGCGAAATCC ATGGACCGCA GGGCAGAGCG AGGACATCGT 
GACCGCACAC CTGAGAGGGA ACACGATGAT GGAGGGTTTC TCTCAGTCAC CCCTGAACGG 
AGGTTCCACG AGCGAAGGCT CCTCAAGGAC TCACAGATAG CTGGACGAGT CAGTGAGGCT 
TCAACCCCCG AGCGCGCCAA CAACAGCATG GAATCCTTGT CTCGTTCCAG GGGCCGCACG 
GACGATAAAG CCACAAAGAA CGGCAACCTT CTGAGAGACT CTTCTTCAGA GAGGAGAGAG 
GACCGACATC GCATGAATGG GACAATGGAG AGGCGATACA GAGCGGAGCG GGACTCTTCC 
CCTGACAGCA ACTACAGCCG GGACGAGCTG AACTATGCAG AAGCACCTAG ACTGAAGGAC 
TACTACAGCA TGATGAAGAA CAACGCTGCA CACAACAATG CTACCTACAA CAACGCAGGC 
CATAACAATA ACACAGTAGG CCGCAGCTCA AACCAGGTCC CTGAGCCCAA GAGGAGGCTG 
TACAAAGAAA GTCCTAAAGA CCTCAGCATC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024860275.1
CDS	894..5726
Translation	MSKVIQKKNHWITKVKECAVVRDPSGELNLELRGGAQDGEFAYIGHIREDAVLHQDGKLSEGELLLEVEGLSVSGLPLYDIYTVINCCKGPVRFKTVRQGHKLNKDLKHFLSLRFQKSSPDHELQKTIRANLYRHAVPCTTRAPRDGEVPGVDYNFLSVEDFLQLEESGTLLEIGTYEGNYYGTPKPPRQPIIGTVTLSDTGSHQSTPKRTKSYNDMQNVRVVPADDDDDDQATEMNNSFTGTLVRSLFPSPLLCTGNPNDQDESRTGILRPYPVRDNALACGLNNATTSTVESGQQTLAEPQVSPDDPLGPLPENWEMAYTENGELYFIDTAKPHHQDMAVHFQSVHNTKTTSWLDPRCRDKASRPLEECDDDEEGIHTEDLESDLELPPGWERIDDPVYGVYYVDHINRKTQYENPVVEARRQKLLEQQQQPQPPEGERYIREWIEEHSAAAAPLANYAPNHLETYRDPQVPPALPPGPAGIKRGKPFFTRNPAELKGTFINTKLKKSHRGFGFTVVGGDEPDEFLQIKSLVLDGPAAVDGKMETGDVIVSVNDTIVLGYTHAQVVKIFQSIPIGSMVDLALCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETFDTPPNQAGPLNSMREPRPLSPSAEVASNGSHGYSSDVVTLASSIATQPELITIHMEKGDKGFGFTIADSLTGGGQRVKQIVDYPRCRGLKEGDILMEVNKRNVQNMSHNQVVDLLSKCPRGSEVTMLVQRGVVPAKRSPKLVHQLERKDSQNSSQHSVCSHRSTHTDSPSHPPSAMPSEAVVPTPAAPTQPLPVLPPQDPADGMLTLQKKPDPFKIWAQSRSMYESRLPDCQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTPGYGGSKGDGDVPPSPASSHHSSTQAPSLTEGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGSAVVPASLQPYDVEIQRGENEGFGFVIVSSVSRPDAGTTFGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVSLRIIPGDESSNASLLTNAEKIATITTTHTPHSTETRNNSKSKGAPPPPPSQTQTQEAEFYSVDLERDSKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKSMKHSRAIELIKNGGRRARLVLKRGDGSVPEYDGSSDGHPPTPRAQNTPEVRTLPPSSRYHTSLESSYPPDLHKSSRLEEAACGRGMDKNRDRAGSDQMEFQGRQFNPHHHYTWNNNHSQSTPRQQSPDSNSTSSGNKKTRGRKVKKSESESGISKLLKTLRKDSSGKKAAAAEKLRGRELSSSGSTLDEKYLRQRRGSLDRSPTRKRTSSPDRRRAKSMDRRAERGHRDRTPEREHDDGGFLSVTPERRFHERRLLKDSQIAGRVSEASTPERANNSMESLSRSRGRTDDKATKNGNLLRDSSSERREDRHRMNGTMERRYRAERDSSPDSNYSRDELNYAEAPRLKDYYSMMKNNAAHNNATYNNAGHNNNTVGRSSNQVPEPKRRLYKESPKDLSI

Target ORF information:

RefSeq Version	XM_025004507.1
Organism	Kryptolebias marmoratus(mangrove rivulus)
Definition	Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025004507.1

ORF Insert Sequence:

ATGTCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACGA AAGTGAAGGA GTGCGCCGTG 
GTTCGGGACC CCAGCGGGGA GCTGAACTTG GAGCTGCGCG GCGGGGCGCA GGACGGAGAG 
TTCGCCTACA TCGGACACAT CAGGGAGGAT GCTGTGCTCC ACCAGGACGG GAAACTGTCT 
GAGGGAGAGC TGCTGCTGGA GGTGGAGGGG CTGTCCGTGT CTGGATTACC TCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAAGGT CCCGTCAGGT TCAAAACTGT CCGCCAAGGT 
CACAAGCTCA ACAAGGACCT AAAGCACTTC CTGAGCTTGA GGTTTCAGAA GTCATCACCT 
GACCACGAGT TGCAGAAGAC TATCCGAGCC AATCTATATC GTCATGCCGT GCCTTGTACA 
ACCCGCGCCC CTCGAGACGG AGAGGTGCCA GGGGTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTTGAGGA GAGTGGCACA CTACTGGAAA TAGGAACGTA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC GCCGAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
ACGGGCTCCC ATCAGTCCAC CCCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAACGTC 
CGCGTAGTGC CTGCTGACGA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGAACCT TAGTGCGTAG CCTTTTCCCC TCTCCTTTGT TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGTAC CGGCATCCTT CGGCCATATC CTGTGCGTGA TAATGCTCTG 
GCCTGTGGTC TGAACAATGC CACCACCTCC ACTGTTGAAA GTGGGCAGCA GACGCTCGCA 
GAACCACAGG TCTCTCCAGA TGACCCTCTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACTGAGA ACGGGGAGCT GTACTTCATA GACACTGCAA AGCCTCACCA CCAGGACATG 
GCTGTGCATT TTCAGAGTGT CCACAACACA AAGACCACAT CATGGCTGGA TCCGCGGTGC 
CGAGACAAAG CCTCCAGGCC TTTGGAAGAG TGTGATGATG ATGAAGAAGG GATACATACG 
GAAGACCTGG AAAGTGATCT AGAGCTGCCC CCAGGATGGG AAAGAATAGA TGACCCCGTG 
TACGGAGTGT ACTATGTAGA TCATATAAAC AGGAAGACCC AATATGAGAA CCCAGTGGTG 
GAGGCGCGGC GCCAAAAACT CTTGGAGCAG CAGCAACAGC CGCAGCCTCC AGAAGGTGAG 
CGGTATATTC GAGAGTGGAT AGAGGAGCAC TCAGCTGCAG CAGCTCCTTT GGCAAACTAT 
GCTCCAAACC ACCTGGAGAC CTACAGGGAC CCACAAGTCC CTCCAGCTCT ACCTCCAGGC 
CCCGCTGGCA TCAAAAGAGG GAAACCTTTC TTTACGAGAA ACCCAGCAGA GCTGAAGGGA 
ACCTTCATCA ACACCAAGCT GAAGAAAAGT CACCGTGGAT TCGGGTTCAC CGTGGTGGGA 
GGTGACGAGC CCGACGAGTT CCTGCAGATC AAGAGTCTGG TGCTGGATGG GCCTGCGGCT 
GTTGATGGCA AGATGGAGAC AGGTGATGTG ATTGTCAGCG TGAATGACAC TATTGTGCTG 
GGCTACACTC ATGCTCAGGT GGTGAAGATC TTCCAGTCCA TCCCTATCGG CTCCATGGTG 
GACCTGGCCT TATGCCGTGG CTACCCACTG CCCTTTGACC CAGACGACCC AAACACCAGC 
CTGGTGACCT CTGTGGCCAT CTTGGACAAG GAACCCATTA TTGTCAATGG GCAAGAGACG 
TTTGACACGC CGCCTAACCA GGCTGGACCC CTTAACAGCA TGCGTGAGCC ACGGCCTCTC 
AGCCCCTCAG CTGAGGTGGC GTCCAACGGC TCCCATGGTT ACTCCAGCGA CGTGGTCACG 
TTGGCCTCGT CCATAGCCAC TCAGCCCGAG CTCATCACCA TCCACATGGA GAAGGGGGAC 
AAAGGTTTTG GCTTCACCAT CGCTGACAGC CTAACAGGCG GAGGCCAGAG GGTAAAGCAG 
ATAGTGGACT ATCCACGGTG CCGCGGCCTG AAAGAGGGGG ACATTCTAAT GGAGGTCAAC 
AAGAGAAATG TCCAGAATAT GAGTCACAAT CAGGTGGTGG ATCTGCTGAG CAAATGTCCA 
AGAGGCAGTG AGGTCACCAT GTTGGTGCAA AGAGGAGTGG TGCCAGCCAA GAGGAGCCCA 
AAGCTGGTGC ACCAACTGGA GAGAAAAGAC AGTCAGAACA GCTCCCAGCA CAGTGTGTGC 
AGCCATCGCA GCACCCACAC CGATTCTCCC AGCCACCCAC CCTCTGCCAT GCCCAGTGAG 
GCAGTGGTCC CCACTCCCGC TGCCCCCACT CAGCCTCTGC CAGTCTTGCC CCCCCAGGAC 
CCCGCAGATG GCATGCTCAC CCTCCAGAAG AAGCCAGACC CTTTTAAGAT CTGGGCACAG 
TCCAGGAGCA TGTATGAGAG TCGACTACCA GATTGCCAGG AGCAAGACAT TTTCCTTTGG 
CGGAAAGATA CAGGTTTTGG GTTTCGCATC TTGGGAGGAA ATGAGCCTGG GGAGCCTATA 
TACATAGGGC ACATAGTGAA GTACGGGGCC GCAGATGAAG ATGGCCGCCT GCGATCGGGT 
GACGAGCTCA TCTGTGTGGA CGGCACGGCT GTAGTGGGCA AATCTCACCA GCTGGTGGTG 
CAGCTGATGC AGCAGGCAGC CAAGCAGGGC CACGTCAACC TCACTGTCAG ACGCAAGACC 
CCCGGATACG GAGGGTCAAA AGGAGATGGT GACGTTCCTC CATCCCCAGC CTCCTCCCAC 
CACAGCAGCA CCCAGGCACC CAGTCTGACG GAGGGAAAGC GCACCCCTCA GGGCAGCCAG 
AACTCACTCA ACACTGTCAG CTCCGGCAGC GGATCCACCA GCGGCATAGG CAGCGGCGGT 
GGAGGAGGCA GTGGGAGCGC GGTGGTGCCT GCCTCCCTGC AGCCTTACGA CGTGGAGATC 
CAGCGAGGAG AGAACGAGGG ATTTGGTTTT GTCATCGTGT CGTCCGTTTC CAGGCCTGAT 
GCTGGCACTA CTTTCGGACG CATCATCGAG GGCAGCCCGG CGGACCGCTG CGGGAAGCTG 
AAGGTCGGGG ACAGAATACT GGCTGTTAAC GGCTGCTCCA TCACCAACAA GTCTCACTCA 
GACATCGTCA ACCTGATTAA GGAAGCCGGG AACACCGTCT CACTCAGGAT CATCCCAGGT 
GATGAATCTT CCAATGCTTC TCTGCTGACT AATGCTGAGA AGATAGCTAC CATCACCACG 
ACACACACAC CTCATTCCAC GGAGACCAGA AATAATTCAA AGTCAAAAGG AGCTCCTCCT 
CCACCCCCTT CACAGACCCA AACACAGGAA GCAGAGTTTT ATTCTGTGGA CCTCGAGAGG 
GACAGTAAAG GCTTCGGCTT CAGCTTAAGA GGAGGACGGG AGTACAACAT GGACCTGTAC 
GTGTTGAGAC TAGCAGAGGA TGGGGCTGCT GTCAGAAATG GCAAGATGAG GGTGGGAGAC 
GAGATCCTGG AGATTAATGG TGAGAGCACA AAGAGCATGA AGCATTCACG AGCCATTGAA 
CTGATCAAGA ACGGCGGTCG GAGGGCACGA CTTGTCCTAA AAAGGGGCGA TGGATCTGTA 
CCTGAATATG ACGGCAGCAG TGACGGGCAC CCTCCCACAC CCCGGGCACA AAACACTCCG 
GAAGTGAGAA CGTTGCCACC CAGCAGCCGA TATCACACCT CATTGGAGTC CAGTTATCCA 
CCAGACCTTC ACAAATCATC ACGCCTGGAG GAGGCTGCTT GTGGCCGAGG TATGGACAAG 
AACAGGGACA GGGCTGGTTC AGATCAGATG GAATTCCAAG GCCGACAATT TAACCCCCAT 
CACCATTACA CTTGGAATAA TAATCACAGT CAAAGTACCC CCAGACAGCA GTCCCCTGAC 
AGTAATAGCA CTAGCAGTGG CAACAAGAAG ACCAGAGGCC GTAAAGTCAA GAAGTCTGAA 
TCGGAGAGTG GCATCTCTAA ACTTCTAAAG ACTCTGAGGA AGGACAGCTC GGGAAAAAAG 
GCTGCAGCCG CCGAAAAACT AAGGGGCCGA GAGCTTTCAA GCAGCGGTTC TACTTTGGAT 
GAGAAGTATC TTCGGCAGCG TCGTGGGTCC CTCGACCGCT CACCAACCAG AAAGCGCACC 
TCGTCTCCAG ATCGTCGACG GGCGAAATCC ATGGACCGCA GGGCAGAGCG AGGACATCGT 
GACCGCACAC CTGAGAGGGA ACACGATGAT GGAGGGTTTC TCTCAGTCAC CCCTGAACGG 
AGGTTCCACG AGCGAAGGCT CCTCAAGGAC TCACAGATAG CTGGACGAGT CAGTGAGGCT 
TCAACCCCCG AGCGCGCCAA CAACAGCATG GAATCCTTGT CTCGTTCCAG GGGCCGCACG 
GACGATAAAG CCACAAAGAA CGGCAACCTT CTGAGAGACT CTTCTTCAGA GAGGAGAGAG 
GACCGACATC GCATGAATGG GACAATGGAG AGGCGATACA GAGCGGAGCG GGACTCTTCC 
CCTGACAGCA ACTACAGCCG GGACGAGCTG AACTATGCAG AAGCACCTAG ACTGAAGGAC 
TACTACAGCA TGATGAAGAA CAACGCTGCA CACAACAATG CTACCTACAA CAACGCAGGC 
CATAACAATA ACACAGTAGG CCGCAGCTCA AACCAGGTCC CTGAGCCCAA GAGGAGGCTG 
TACAAAGAAA GTCCTAAAGA CCTCAGCATC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108232031 ( XM_025004508.1 ) cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus) -> XP_024860276.1 Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X7, mRNA.

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CloneID	OKr34861
Clone ID Related Accession (Same CDS sequence)	XM_025004508.1
Accession Version	XM_025004508.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-03
Organism	Kryptolebias marmoratus(mangrove rivulus)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016094253.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Kryptolebias marmoratus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACGA AAGTGAAGGA GTGCGCCGTG 
GTTCGGGACC CCAGCGGGGA GCTGAACTTG GAGCTGCGCG GCGGGGCGCA GGACGGAGAG 
TTCGCCTACA TCGGACACAT CAGGGAGGAT GCTGTGCTCC ACCAGGACGG GAAACTGTCT 
GAGGGAGAGC TGCTGCTGGA GGTGGAGGGG CTGTCCGTGT CTGGATTACC TCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAAGGT CCCGTCAGGT TCAAAACTGT CCGCCAAGGT 
CACAAGCTCA ACAAGGACCT AAAGCACTTC CTGAGCTTGA GGTTTCAGAA GTCATCACCT 
GACCACGAGT TGCAGAAGAC TATCCGAGCC AATCTATATC GTCATGCCGT GCCTTGTACA 
ACCCGCGCCC CTCGAGACGG AGAGGTGCCA GGGGTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTTGAGGA GAGTGGCACA CTACTGGAAA TAGGAACGTA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC GCCGAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
ACGGGCTCCC ATCAGTCCAC CCCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAACGTC 
CGCGTAGTGC CTGCTGACGA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGAACCT TAGTGCGTAG CCTTTTCCCC TCTCCTTTGT TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGTAC CGGCATCCTT CGGCCATATC CTGTGCGTGA TAATGCTCTG 
GCCTGTGGTC TGAACAATGC CACCACCTCC ACTGTTGAAA GTGGGCAGCA GACGCTCGCA 
GAACCACAGG TCTCTCCAGA TGACCCTCTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACTGAGA ACGGGGAGCT GTACTTCATA GACCACAACA CAAAGACCAC ATCATGGCTG 
GATCCGCGGT GCCGAGACAA AGCCTCCAGG CCTTTGGAAG AGTGTGATGA TGATGAAGGG 
ATACATACGG AAGACCTGGA AAGTGATCTA GAGCTGCCCC CAGGATGGGA AAGAATAGAT 
GACCCCGTGT ACGGAGTGTA CTATGTAGAT CATATAAACA GGAAGACCCA ATATGAGAAC 
CCAGTGGTGG AGGCGCGGCG CCAAAAACTC TTGGAGCAGC AGCAACAGCC GCAGCCTCCA 
GAAGGTGAGC GGTATATTCG AGAGTGGATA GAGGAGCACT CAGCTGCAGC AGCTCCTTTG 
GCAAACTATG CTCCAAACCA CCTGGAGACC TACAGGGACC CACAAGTCCC TCCAGCTCTA 
CCTCCAGGCC CCGCTGGCAT CAAAAGAGGG AAACCTTTCT TTACGAGAAA CCCAGCAGAG 
CTGAAGGGAA CCTTCATCAA CACCAAGCTG AAGAAAAGTC ACCGTGGATT CGGGTTCACC 
GTGGTGGGAG GTGACGAGCC CGACGAGTTC CTGCAGATCA AGAGTCTGGT GCTGGATGGG 
CCTGCGGCTG TTGATGGCAA GATGGAGACA GGTGATGTGA TTGTCAGCGT GAATGACACT 
ATTGTGCTGG GCTACACTCA TGCTCAGGTG GTGAAGATCT TCCAGTCCAT CCCTATCGGC 
TCCATGGTGG ACCTGGCCTT ATGCCGTGGC TACCCACTGC CCTTTGACCC AGACGACCCA 
AACACCAGCC TGGTGACCTC TGTGGCCATC TTGGACAAGG AACCCATTAT TGTCAATGGG 
CAAGAGACGT TTGACACGCC GCCTAACCAG GCTGGACCCC TTAACAGCAT GCGTGAGCCA 
CGGCCTCTCA GCCCCTCAGC TGAGGTGGCG TCCAACGGCT CCCATGGTTA CTCCAGCGAC 
GTGGTCACGT TGGCCTCGTC CATAGCCACT CAGCCCGAGC TCATCACCAT CCACATGGAG 
AAGGGGGACA AAGGTTTTGG CTTCACCATC GCTGACAGCC TAACAGGCGG AGGCCAGAGG 
GTAAAGCAGA TAGTGGACTA TCCACGGTGC CGCGGCCTGA AAGAGGGGGA CATTCTAATG 
GAGGTCAACA AGAGAAATGT CCAGAATATG AGTCACAATC AGGTGGTGGA TCTGCTGAGC 
AAATGTCCAA GAGGCAGTGA GGTCACCATG TTGGTGCAAA GAGGAGTGGT GCCAGCCAAG 
AGGAGCCCAA AGCTGGTGCA CCAACTGGAG AGAAAAGACA GTCAGAACAG CTCCCAGCAC 
AGTGTGTGCA GCCATCGCAG CACCCACACC GATTCTCCCA GCCACCCACC CTCTGCCATG 
CCCAGTGAGG CAGTGGTCCC CACTCCCGCT GCCCCCACTC AGCCTCTGCC AGTCTTGCCC 
CCCCAGGACC CCGCAGATGG CATGCTCACC CTCCAGAAGA AGCCAGACCC TTTTAAGATC 
TGGGCACAGT CCAGGAGCAT GTATGAGAGT CGACTACCAG ATTGCCAGGA GCAAGACATT 
TTCCTTTGGC GGAAAGATAC AGGTTTTGGG TTTCGCATCT TGGGAGGAAA TGAGCCTGGG 
GAGCCTATAT ACATAGGGCA CATAGTGAAG TACGGGGCCG CAGATGAAGA TGGCCGCCTG 
CGATCGGGTG ACGAGCTCAT CTGTGTGGAC GGCACGGCTG TAGTGGGCAA ATCTCACCAG 
CTGGTGGTGC AGCTGATGCA GCAGGCAGCC AAGCAGGGCC ACGTCAACCT CACTGTCAGA 
CGCAAGACCC CCGGATACGG AGGGTCAAAA GGAGATGGTG ACGTTCCTCC ATCCCCAGCC 
TCCTCCCACC ACAGCAGCAC CCAGGCACCC AGTCTGACGG AGGGAAAGCG CACCCCTCAG 
GGCAGCCAGA ACTCACTCAA CACTGTCAGC TCCGGCAGCG GATCCACCAG CGGCATAGGC 
AGCGGCGGTG GAGGAGGCAG TGGGAGCGCG GTGGTGCCTG CCTCCCTGCA GCCTTACGAC 
GTGGAGATCC AGCGAGGAGA GAACGAGGGA TTTGGTTTTG TCATCGTGTC GTCCGTTTCC 
AGGCCTGATG CTGGCACTAC TTTCGCTGGA AATGCTTGTG TGGCAATGCC CCACAAAATA 
GGACGCATCA TCGAGGGCAG CCCGGCGGAC CGCTGCGGGA AGCTGAAGGT CGGGGACAGA 
ATACTGGCTG TTAACGGCTG CTCCATCACC AACAAGTCTC ACTCAGACAT CGTCAACCTG 
ATTAAGGAAG CCGGGAACAC CGTCTCACTC AGGATCATCC CAGGTGATGA ATCTTCCAAT 
GCTTCTCTGC TGACTAATGC TGAGAAGATA GCTACCATCA CCACGACACA CACACCTCAT 
TCCACGGAGA CCAGAAATAA TTCAAAGTCA AAAGGAGCTC CTCCTCCACC CCCTTCACAG 
ACCCAAACAC AGGAAGCAGA GTTTTATTCT GTGGACCTCG AGAGGGACAG TAAAGGCTTC 
GGCTTCAGCT TAAGAGGAGG ACGGGAGTAC AACATGGACC TGTACGTGTT GAGACTAGCA 
GAGGATGGGG CTGCTGTCAG AAATGGCAAG ATGAGGGTGG GAGACGAGAT CCTGGAGATT 
AATGGTGAGA GCACAAAGAG CATGAAGCAT TCACGAGCCA TTGAACTGAT CAAGAACGGC 
GGTCGGAGGG CACGACTTGT CCTAAAAAGG GGCGATGGAT CTGTACCTGA ATATGACGGC 
AGCAGTGACG GGCACCCTCC CACACCCCGG GCACAAAACA CTCCGGAAGT GAGAACGTTG 
CCACCCAGCA GCCGATATCA CACCTCATTG GAGTCCAGTT ATCCACCAGA CCTTCACAAA 
TCATCACGCC TGGAGGAGGC TGCTTGTGGC CGAGGTATGG ACAAGAACAG GGACAGGGCT 
GGTTCAGATC AGATGGAATT CCAAGGCCGA CAATTTAACC CCCATCACCA TTACACTTGG 
AATAATAATC ACAGTCAAAG TACCCCCAGA CAGCAGTCCC CTGACAGTAA TAGCACTAGC 
AGTGGCAACA AGAAGACCAG AGGCCGTAAA GTCAAGAAGT CTGAATCGGA GAGTGGCATC 
TCTAAACTTC TAAAGACTCT GAGGAAGGAC AGCTCGGGAA AAAAGGCTGC AGCCGCCGAA 
AAACTAAGGG GCCGAGAGCT TTCAAGCAGC GGTTCTACTT TGGATGAGAA GTATCTTCGG 
CAGCGTCGTG GGTCCCTCGA CCGCTCACCA ACCAGAAAGC GCACCTCGTC TCCAGATCGT 
CGACGGGCGA AATCCATGGA CCGCAGGGCA GAGCGAGGAC ATCGTGACCG CACACCTGAG 
AGGGAACACG ATGATGGAGG GTTTCTCTCA GTCACCCCTG AACGGAGGTT CCACGAGCGA 
AGGCTCCTCA AGGACTCACA GATAGCTGGA CGAGTCAGTG AGGCTTCAAC CCCCGAGCGC 
GCCAACAACA GCATGGAATC CTTGTCTCGT TCCAGGGGCC GCACGGACGA TAAAGCCACA 
AAGAACGGCA ACCTTCTGAG AGACTCTTCT TCAGAGAGGA GAGAGGACCG ACATCGCATG 
AATGGGACAA TGGAGAGGCG ATACAGAGCG GAGCGGGACT CTTCCCCTGA CAGCAACTAC 
AGCCGGGACG AGCTGAACTA TGCAGAAGCA CCTAGACTGA AGGACTACTA CAGCATGATG 
AAGAACAACG CTGCACACAA CAATGCTACC TACAACAACG CAGGCCATAA CAATAACACA 
GTAGGCCGCA GCTCAAACCA GGTCCCTGAG CCCAAGAGGA GGCTGTACAA AGAAAGTCCT 
AAAGACCTCA GCATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024860276.1
CDS	892..5709
Translation	MSKVIQKKNHWITKVKECAVVRDPSGELNLELRGGAQDGEFAYIGHIREDAVLHQDGKLSEGELLLEVEGLSVSGLPLYDIYTVINCCKGPVRFKTVRQGHKLNKDLKHFLSLRFQKSSPDHELQKTIRANLYRHAVPCTTRAPRDGEVPGVDYNFLSVEDFLQLEESGTLLEIGTYEGNYYGTPKPPRQPIIGTVTLSDTGSHQSTPKRTKSYNDMQNVRVVPADDDDDDQATEMNNSFTGTLVRSLFPSPLLCTGNPNDQDESRTGILRPYPVRDNALACGLNNATTSTVESGQQTLAEPQVSPDDPLGPLPENWEMAYTENGELYFIDHNTKTTSWLDPRCRDKASRPLEECDDDEGIHTEDLESDLELPPGWERIDDPVYGVYYVDHINRKTQYENPVVEARRQKLLEQQQQPQPPEGERYIREWIEEHSAAAAPLANYAPNHLETYRDPQVPPALPPGPAGIKRGKPFFTRNPAELKGTFINTKLKKSHRGFGFTVVGGDEPDEFLQIKSLVLDGPAAVDGKMETGDVIVSVNDTIVLGYTHAQVVKIFQSIPIGSMVDLALCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETFDTPPNQAGPLNSMREPRPLSPSAEVASNGSHGYSSDVVTLASSIATQPELITIHMEKGDKGFGFTIADSLTGGGQRVKQIVDYPRCRGLKEGDILMEVNKRNVQNMSHNQVVDLLSKCPRGSEVTMLVQRGVVPAKRSPKLVHQLERKDSQNSSQHSVCSHRSTHTDSPSHPPSAMPSEAVVPTPAAPTQPLPVLPPQDPADGMLTLQKKPDPFKIWAQSRSMYESRLPDCQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTPGYGGSKGDGDVPPSPASSHHSSTQAPSLTEGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGSAVVPASLQPYDVEIQRGENEGFGFVIVSSVSRPDAGTTFAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVSLRIIPGDESSNASLLTNAEKIATITTTHTPHSTETRNNSKSKGAPPPPPSQTQTQEAEFYSVDLERDSKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKSMKHSRAIELIKNGGRRARLVLKRGDGSVPEYDGSSDGHPPTPRAQNTPEVRTLPPSSRYHTSLESSYPPDLHKSSRLEEAACGRGMDKNRDRAGSDQMEFQGRQFNPHHHYTWNNNHSQSTPRQQSPDSNSTSSGNKKTRGRKVKKSESESGISKLLKTLRKDSSGKKAAAAEKLRGRELSSSGSTLDEKYLRQRRGSLDRSPTRKRTSSPDRRRAKSMDRRAERGHRDRTPEREHDDGGFLSVTPERRFHERRLLKDSQIAGRVSEASTPERANNSMESLSRSRGRTDDKATKNGNLLRDSSSERREDRHRMNGTMERRYRAERDSSPDSNYSRDELNYAEAPRLKDYYSMMKNNAAHNNATYNNAGHNNNTVGRSSNQVPEPKRRLYKESPKDLSI

Target ORF information:

RefSeq Version	XM_025004508.1
Organism	Kryptolebias marmoratus(mangrove rivulus)
Definition	Kryptolebias marmoratus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC108232031), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025004508.1

ORF Insert Sequence:

ATGTCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACGA AAGTGAAGGA GTGCGCCGTG 
GTTCGGGACC CCAGCGGGGA GCTGAACTTG GAGCTGCGCG GCGGGGCGCA GGACGGAGAG 
TTCGCCTACA TCGGACACAT CAGGGAGGAT GCTGTGCTCC ACCAGGACGG GAAACTGTCT 
GAGGGAGAGC TGCTGCTGGA GGTGGAGGGG CTGTCCGTGT CTGGATTACC TCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAAGGT CCCGTCAGGT TCAAAACTGT CCGCCAAGGT 
CACAAGCTCA ACAAGGACCT AAAGCACTTC CTGAGCTTGA GGTTTCAGAA GTCATCACCT 
GACCACGAGT TGCAGAAGAC TATCCGAGCC AATCTATATC GTCATGCCGT GCCTTGTACA 
ACCCGCGCCC CTCGAGACGG AGAGGTGCCA GGGGTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTTGAGGA GAGTGGCACA CTACTGGAAA TAGGAACGTA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC GCCGAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
ACGGGCTCCC ATCAGTCCAC CCCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAACGTC 
CGCGTAGTGC CTGCTGACGA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGAACCT TAGTGCGTAG CCTTTTCCCC TCTCCTTTGT TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGTAC CGGCATCCTT CGGCCATATC CTGTGCGTGA TAATGCTCTG 
GCCTGTGGTC TGAACAATGC CACCACCTCC ACTGTTGAAA GTGGGCAGCA GACGCTCGCA 
GAACCACAGG TCTCTCCAGA TGACCCTCTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACTGAGA ACGGGGAGCT GTACTTCATA GACCACAACA CAAAGACCAC ATCATGGCTG 
GATCCGCGGT GCCGAGACAA AGCCTCCAGG CCTTTGGAAG AGTGTGATGA TGATGAAGGG 
ATACATACGG AAGACCTGGA AAGTGATCTA GAGCTGCCCC CAGGATGGGA AAGAATAGAT 
GACCCCGTGT ACGGAGTGTA CTATGTAGAT CATATAAACA GGAAGACCCA ATATGAGAAC 
CCAGTGGTGG AGGCGCGGCG CCAAAAACTC TTGGAGCAGC AGCAACAGCC GCAGCCTCCA 
GAAGGTGAGC GGTATATTCG AGAGTGGATA GAGGAGCACT CAGCTGCAGC AGCTCCTTTG 
GCAAACTATG CTCCAAACCA CCTGGAGACC TACAGGGACC CACAAGTCCC TCCAGCTCTA 
CCTCCAGGCC CCGCTGGCAT CAAAAGAGGG AAACCTTTCT TTACGAGAAA CCCAGCAGAG 
CTGAAGGGAA CCTTCATCAA CACCAAGCTG AAGAAAAGTC ACCGTGGATT CGGGTTCACC 
GTGGTGGGAG GTGACGAGCC CGACGAGTTC CTGCAGATCA AGAGTCTGGT GCTGGATGGG 
CCTGCGGCTG TTGATGGCAA GATGGAGACA GGTGATGTGA TTGTCAGCGT GAATGACACT 
ATTGTGCTGG GCTACACTCA TGCTCAGGTG GTGAAGATCT TCCAGTCCAT CCCTATCGGC 
TCCATGGTGG ACCTGGCCTT ATGCCGTGGC TACCCACTGC CCTTTGACCC AGACGACCCA 
AACACCAGCC TGGTGACCTC TGTGGCCATC TTGGACAAGG AACCCATTAT TGTCAATGGG 
CAAGAGACGT TTGACACGCC GCCTAACCAG GCTGGACCCC TTAACAGCAT GCGTGAGCCA 
CGGCCTCTCA GCCCCTCAGC TGAGGTGGCG TCCAACGGCT CCCATGGTTA CTCCAGCGAC 
GTGGTCACGT TGGCCTCGTC CATAGCCACT CAGCCCGAGC TCATCACCAT CCACATGGAG 
AAGGGGGACA AAGGTTTTGG CTTCACCATC GCTGACAGCC TAACAGGCGG AGGCCAGAGG 
GTAAAGCAGA TAGTGGACTA TCCACGGTGC CGCGGCCTGA AAGAGGGGGA CATTCTAATG 
GAGGTCAACA AGAGAAATGT CCAGAATATG AGTCACAATC AGGTGGTGGA TCTGCTGAGC 
AAATGTCCAA GAGGCAGTGA GGTCACCATG TTGGTGCAAA GAGGAGTGGT GCCAGCCAAG 
AGGAGCCCAA AGCTGGTGCA CCAACTGGAG AGAAAAGACA GTCAGAACAG CTCCCAGCAC 
AGTGTGTGCA GCCATCGCAG CACCCACACC GATTCTCCCA GCCACCCACC CTCTGCCATG 
CCCAGTGAGG CAGTGGTCCC CACTCCCGCT GCCCCCACTC AGCCTCTGCC AGTCTTGCCC 
CCCCAGGACC CCGCAGATGG CATGCTCACC CTCCAGAAGA AGCCAGACCC TTTTAAGATC 
TGGGCACAGT CCAGGAGCAT GTATGAGAGT CGACTACCAG ATTGCCAGGA GCAAGACATT 
TTCCTTTGGC GGAAAGATAC AGGTTTTGGG TTTCGCATCT TGGGAGGAAA TGAGCCTGGG 
GAGCCTATAT ACATAGGGCA CATAGTGAAG TACGGGGCCG CAGATGAAGA TGGCCGCCTG 
CGATCGGGTG ACGAGCTCAT CTGTGTGGAC GGCACGGCTG TAGTGGGCAA ATCTCACCAG 
CTGGTGGTGC AGCTGATGCA GCAGGCAGCC AAGCAGGGCC ACGTCAACCT CACTGTCAGA 
CGCAAGACCC CCGGATACGG AGGGTCAAAA GGAGATGGTG ACGTTCCTCC ATCCCCAGCC 
TCCTCCCACC ACAGCAGCAC CCAGGCACCC AGTCTGACGG AGGGAAAGCG CACCCCTCAG 
GGCAGCCAGA ACTCACTCAA CACTGTCAGC TCCGGCAGCG GATCCACCAG CGGCATAGGC 
AGCGGCGGTG GAGGAGGCAG TGGGAGCGCG GTGGTGCCTG CCTCCCTGCA GCCTTACGAC 
GTGGAGATCC AGCGAGGAGA GAACGAGGGA TTTGGTTTTG TCATCGTGTC GTCCGTTTCC 
AGGCCTGATG CTGGCACTAC TTTCGCTGGA AATGCTTGTG TGGCAATGCC CCACAAAATA 
GGACGCATCA TCGAGGGCAG CCCGGCGGAC CGCTGCGGGA AGCTGAAGGT CGGGGACAGA 
ATACTGGCTG TTAACGGCTG CTCCATCACC AACAAGTCTC ACTCAGACAT CGTCAACCTG 
ATTAAGGAAG CCGGGAACAC CGTCTCACTC AGGATCATCC CAGGTGATGA ATCTTCCAAT 
GCTTCTCTGC TGACTAATGC TGAGAAGATA GCTACCATCA CCACGACACA CACACCTCAT 
TCCACGGAGA CCAGAAATAA TTCAAAGTCA AAAGGAGCTC CTCCTCCACC CCCTTCACAG 
ACCCAAACAC AGGAAGCAGA GTTTTATTCT GTGGACCTCG AGAGGGACAG TAAAGGCTTC 
GGCTTCAGCT TAAGAGGAGG ACGGGAGTAC AACATGGACC TGTACGTGTT GAGACTAGCA 
GAGGATGGGG CTGCTGTCAG AAATGGCAAG ATGAGGGTGG GAGACGAGAT CCTGGAGATT 
AATGGTGAGA GCACAAAGAG CATGAAGCAT TCACGAGCCA TTGAACTGAT CAAGAACGGC 
GGTCGGAGGG CACGACTTGT CCTAAAAAGG GGCGATGGAT CTGTACCTGA ATATGACGGC 
AGCAGTGACG GGCACCCTCC CACACCCCGG GCACAAAACA CTCCGGAAGT GAGAACGTTG 
CCACCCAGCA GCCGATATCA CACCTCATTG GAGTCCAGTT ATCCACCAGA CCTTCACAAA 
TCATCACGCC TGGAGGAGGC TGCTTGTGGC CGAGGTATGG ACAAGAACAG GGACAGGGCT 
GGTTCAGATC AGATGGAATT CCAAGGCCGA CAATTTAACC CCCATCACCA TTACACTTGG 
AATAATAATC ACAGTCAAAG TACCCCCAGA CAGCAGTCCC CTGACAGTAA TAGCACTAGC 
AGTGGCAACA AGAAGACCAG AGGCCGTAAA GTCAAGAAGT CTGAATCGGA GAGTGGCATC 
TCTAAACTTC TAAAGACTCT GAGGAAGGAC AGCTCGGGAA AAAAGGCTGC AGCCGCCGAA 
AAACTAAGGG GCCGAGAGCT TTCAAGCAGC GGTTCTACTT TGGATGAGAA GTATCTTCGG 
CAGCGTCGTG GGTCCCTCGA CCGCTCACCA ACCAGAAAGC GCACCTCGTC TCCAGATCGT 
CGACGGGCGA AATCCATGGA CCGCAGGGCA GAGCGAGGAC ATCGTGACCG CACACCTGAG 
AGGGAACACG ATGATGGAGG GTTTCTCTCA GTCACCCCTG AACGGAGGTT CCACGAGCGA 
AGGCTCCTCA AGGACTCACA GATAGCTGGA CGAGTCAGTG AGGCTTCAAC CCCCGAGCGC 
GCCAACAACA GCATGGAATC CTTGTCTCGT TCCAGGGGCC GCACGGACGA TAAAGCCACA 
AAGAACGGCA ACCTTCTGAG AGACTCTTCT TCAGAGAGGA GAGAGGACCG ACATCGCATG 
AATGGGACAA TGGAGAGGCG ATACAGAGCG GAGCGGGACT CTTCCCCTGA CAGCAACTAC 
AGCCGGGACG AGCTGAACTA TGCAGAAGCA CCTAGACTGA AGGACTACTA CAGCATGATG 
AAGAACAACG CTGCACACAA CAATGCTACC TACAACAACG CAGGCCATAA CAATAACACA 
GTAGGCCGCA GCTCAAACCA GGTCCCTGAG CCCAAGAGGA GGCTGTACAA AGAAAGTCCT 
AAAGACCTCA GCATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

LOC108232031 cDNA ORF clone, Kryptolebias marmoratus(mangrove rivulus)

Gene

Clones

gRNAs

Target ORF information:

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ORF Insert Sequence:

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