DGAT1 cDNA ORF clone, Jatropha curcas

Gene
Clones
gRNAs

Summary

Gene Symbol	DGAT1
Entrez Gene ID	105637897
Full Name	diacylglycerol O-acyltransferase 1
Synonyms	DGAT-1
Gene Type	protein-coding
Organism	Jatropha curcas

mRNA and Protein(s)

mRNA	Protein	Name
NM_001305997.1	NP_001292926.1	diacylglycerol O-acyltransferase 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Global analysis of transcriptome responses and gene expression profiles to cold stress of Jatropha curcas L.
Wang H, Zou Z, Wang S, Gong M
PloS one8(12)e82817(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DGAT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DGAT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OJa80474	NM_001305997.1 Latest version!	Jatropha curcas diacylglycerol O-acyltransferase 1 (DGAT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DGAT1 ( NM_001305997.1 ) cDNA ORF clone, Jatropha curcas -> NP_001292926.1 Jatropha curcas diacylglycerol O-acyltransferase 1 (DGAT1), mRNA.

Price & Availability↑

CloneID	OJa80474
Clone ID Related Accession (Same CDS sequence)	NM_001305997.1
Accession Version	NM_001305997.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-08
Organism	Jatropha curcas
Product	diacylglycerol O-acyltransferase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ278448.1. On Apr 10, 2015 this sequence version replaced XM_012221564.1. ##Evidence-Data-START## Transcript exon combination :: DQ278448.1, EU477378.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01894327, SAMN02350404 [ECO:0000348] ##Evidence-Data-END##

ATGACGATTT TGGAGACCAC TACTAGCGGA GGTGATGGTG TTGCTGAGTC GTCTTCCGAT 
CTTAACGTAT CGCTTCGACG GAGACGGAAA GGCACCAGCT CGGATGGAGC TTTGCCGGAA 
TTGACTTCGA ATATTGTTGA ATTGGAATCT GAAAGCGGTG GCCAGGTGAT GATGGATCCA 
GGTATGGTGA CGGAACCGGA GACAGAGAAA ATTAATGGAA AAGATTGCGG CGGTGACAAG 
GATAAGATTG ACAATCGCGA GAATCGTGGG AGGTCGGATA TTAAATTCAC GTACCGGCCA 
TCGGTGCCGG CTCATCGAGC GCTCAGGGAG AGTCCGCTTA GCTCTGATGC TATATTTAAA 
CAAAGTCATG CAGGTCTGTT CAACCTCTGT ATAGTAGTGC TTGTTGCTGT TAACAGCAGG 
CTTATCATTG AAAATCTAAT GAAGTATGGT TGGTTAATTA AAACGGGGTT TTGGTTTAGT 
TCAAGATCGT TGAGAGATTG GCCCCTTCTT ATGTGCTGTC TTACCCTCCC TATATTCTCT 
CTTGCCGCCT ATCTAGTTGA GAAGTTGGCA TATCGAAAAT ATATATCTGC ACCTATTGTT 
ATTTTCTTTC ATATGCTCAT TACCACAACA GCAGTTTTGT ACCCAGTTTC TGTGATTCTC 
AGTTGTGGGT CTGCTGTTCT GTCTGGTGTT GCATTGATGC TCTTTGCTTG TATCGTGTGG 
TTGAAATTAG TATCTTATGC ACATACAAAC TATGACATGA GAGCCATTGC CAACTCAGCT 
GACAAGGGAG ATGCACTATC CGATACTTCA GGTGCAGATT CTTCACGTGA TGTTAGCTTC 
AAGAGTTTGG TCTACTTCAT GGTTGCTCCT ACGCTATGTT ACCAGCCAAG TTATCCTCGA 
ACAGATTCAG TTAGAAAGGG TTGGGTGGTT CGTCAATTTG TCAAGTTAAT AATATTTACA 
GGATTCATGG GATTTATCAT AGAACAATAT ATCAATCCTA TTGTCCAGAA TTCACAACAC 
CCCTTAAAGG GGGATCTATT ATATGCCATT GAAAGGGTTT TGAAGCTCTC AGTTCCAAAC 
TTATATGTGT GGCTCTGCAT GTTCTACTGC TTTTTTCATC TATGGTTAAA TATACTTGCT 
GAGCTCCTTC GGTTTGGTGA CAGAGAGTTC TATAAAGATT GGTGGAATGC AAGGACAGTT 
GAGGAGTACT GGAGAATGTG GAATATGCCT GTTCATAAGT GGATGGTTCG CCATATCTAC 
TTTCCATGCT TGCGGCATAA AATACCAAGG GGGGTAGCCT TGTTAATTGC TTTCTTCGTT 
TCAGCTGTAT TTCATGAGTT GTGCATTGCT GTTCCTTGCC ACATGTTCAA GCTCTGGGCT 
TTTATTGGAA TTATGTTTCA GATTCCATTG GTCGGGATCA CTAATTACCT CCAGAACAAG 
TTCAGAAGCT CCATGGTGGG AAATATGATC TTTTGGTTCA TTTTCTGCAT TCTTGGTCAA 
CCCATGTGTG TGCTATTGTA TTATCATGAC CTAATGAATC GGAAAGGCAA TGCTGAATTA 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292926.1
CDS	1..1566
Translation	MTILETTTSGGDGVAESSSDLNVSLRRRRKGTSSDGALPELTSNIVELESESGGQVMMDPGMVTEPETEKINGKDCGGDKDKIDNRENRGRSDIKFTYRPSVPAHRALRESPLSSDAIFKQSHAGLFNLCIVVLVAVNSRLIIENLMKYGWLIKTGFWFSSRSLRDWPLLMCCLTLPIFSLAAYLVEKLAYRKYISAPIVIFFHMLITTTAVLYPVSVILSCGSAVLSGVALMLFACIVWLKLVSYAHTNYDMRAIANSADKGDALSDTSGADSSRDVSFKSLVYFMVAPTLCYQPSYPRTDSVRKGWVVRQFVKLIIFTGFMGFIIEQYINPIVQNSQHPLKGDLLYAIERVLKLSVPNLYVWLCMFYCFFHLWLNILAELLRFGDREFYKDWWNARTVEEYWRMWNMPVHKWMVRHIYFPCLRHKIPRGVALLIAFFVSAVFHELCIAVPCHMFKLWAFIGIMFQIPLVGITNYLQNKFRSSMVGNMIFWFIFCILGQPMCVLLYYHDLMNRKGNAELR

Target ORF information:

RefSeq Version	NM_001305997.1
Organism	Jatropha curcas
Definition	Jatropha curcas diacylglycerol O-acyltransferase 1 (DGAT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305997.1

ORF Insert Sequence:

ATGACGATTT TGGAGACCAC TACTAGCGGA GGTGATGGTG TTGCTGAGTC GTCTTCCGAT 
CTTAACGTAT CGCTTCGACG GAGACGGAAA GGCACCAGCT CGGATGGAGC TTTGCCGGAA 
TTGACTTCGA ATATTGTTGA ATTGGAATCT GAAAGCGGTG GCCAGGTGAT GATGGATCCA 
GGTATGGTGA CGGAACCGGA GACAGAGAAA ATTAATGGAA AAGATTGCGG CGGTGACAAG 
GATAAGATTG ACAATCGCGA GAATCGTGGG AGGTCGGATA TTAAATTCAC GTACCGGCCA 
TCGGTGCCGG CTCATCGAGC GCTCAGGGAG AGTCCGCTTA GCTCTGATGC TATATTTAAA 
CAAAGTCATG CAGGTCTGTT CAACCTCTGT ATAGTAGTGC TTGTTGCTGT TAACAGCAGG 
CTTATCATTG AAAATCTAAT GAAGTATGGT TGGTTAATTA AAACGGGGTT TTGGTTTAGT 
TCAAGATCGT TGAGAGATTG GCCCCTTCTT ATGTGCTGTC TTACCCTCCC TATATTCTCT 
CTTGCCGCCT ATCTAGTTGA GAAGTTGGCA TATCGAAAAT ATATATCTGC ACCTATTGTT 
ATTTTCTTTC ATATGCTCAT TACCACAACA GCAGTTTTGT ACCCAGTTTC TGTGATTCTC 
AGTTGTGGGT CTGCTGTTCT GTCTGGTGTT GCATTGATGC TCTTTGCTTG TATCGTGTGG 
TTGAAATTAG TATCTTATGC ACATACAAAC TATGACATGA GAGCCATTGC CAACTCAGCT 
GACAAGGGAG ATGCACTATC CGATACTTCA GGTGCAGATT CTTCACGTGA TGTTAGCTTC 
AAGAGTTTGG TCTACTTCAT GGTTGCTCCT ACGCTATGTT ACCAGCCAAG TTATCCTCGA 
ACAGATTCAG TTAGAAAGGG TTGGGTGGTT CGTCAATTTG TCAAGTTAAT AATATTTACA 
GGATTCATGG GATTTATCAT AGAACAATAT ATCAATCCTA TTGTCCAGAA TTCACAACAC 
CCCTTAAAGG GGGATCTATT ATATGCCATT GAAAGGGTTT TGAAGCTCTC AGTTCCAAAC 
TTATATGTGT GGCTCTGCAT GTTCTACTGC TTTTTTCATC TATGGTTAAA TATACTTGCT 
GAGCTCCTTC GGTTTGGTGA CAGAGAGTTC TATAAAGATT GGTGGAATGC AAGGACAGTT 
GAGGAGTACT GGAGAATGTG GAATATGCCT GTTCATAAGT GGATGGTTCG CCATATCTAC 
TTTCCATGCT TGCGGCATAA AATACCAAGG GGGGTAGCCT TGTTAATTGC TTTCTTCGTT 
TCAGCTGTAT TTCATGAGTT GTGCATTGCT GTTCCTTGCC ACATGTTCAA GCTCTGGGCT 
TTTATTGGAA TTATGTTTCA GATTCCATTG GTCGGGATCA CTAATTACCT CCAGAACAAG 
TTCAGAAGCT CCATGGTGGG AAATATGATC TTTTGGTTCA TTTTCTGCAT TCTTGGTCAA 
CCCATGTGTG TGCTATTGTA TTATCATGAC CTAATGAATC GGAAAGGCAA TGCTGAATTA 
AGATGA