EKO cDNA ORF clone, Jatropha curcas

Gene
Clones
gRNAs

Summary

Gene Symbol	EKO
Entrez Gene ID	105633700
Full Name	ent-kaurene oxidase, chloroplastic
Gene Type	protein-coding
Organism	Jatropha curcas

mRNA and Protein(s)

mRNA	Protein	Name
XM_012216337.2	XP_012071727.1	ent-kaurene oxidase, chloroplastic isoform X1
NM_001306026.1	NP_001292955.1	ent-kaurene oxidase, chloroplastic

Pathways from BioSystems
Gene Ontology

The following EKO gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EKO cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OJa48725	XM_012216337.2 Latest version!	Jatropha curcas ent-kaurene oxidase, chloroplastic (EKO), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OJa80471	NM_001306026.1 Latest version!	Jatropha curcas ent-kaurene oxidase, chloroplastic (EKO), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EKO ( XM_012216337.2 ) cDNA ORF clone, Jatropha curcas -> XP_012071727.1 Jatropha curcas ent-kaurene oxidase, chloroplastic (EKO), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OJa48725
Clone ID Related Accession (Same CDS sequence)	XM_012216337.2
Accession Version	XM_012216337.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-05
Organism	Jatropha curcas
Product	ent-kaurene oxidase, chloroplastic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012124217.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 6, 2017 this sequence version replaced XM_012216337.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Jatropha curcas Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTACAAC ATGCCATGGT GACTAGATAT TCATCCATCT CAACAAGGAA ACTATCAAAA 
GCCTTGTCTG TGCTTACCCA GGATAAATGT ATGGTTGCTA CAAGTGATTA TGATGAGTTT 
CACAAGAATG CAAAACGCTG TATACTTACG AATGTATTGG GACCAAATGC CCAGAGGCGA 
CATCGACCCC ACAGGGACAC CCTGGTTGAA AATATTTCAA CCCGGTTTCA TGCCCATTTA 
AAGAGCTATC CTGAACAAGC AGTAAATTTC AGAGAAGTAT TTGAGTCTGA ACTTTTTGGG 
GTATCACTGA AAGAAGCATT TGGAGAGGAT GTGCAATCTG TTTATGTTGA CGACTTGAGG 
ACCACTTTAA CAAGAGAGGA GATTTTCGAA ATTTTAGTGC TTGATCCCAT GCAGGGTGCA 
ATTGATGTGG ACTGGAGAGA TTTTTTCCCA TATTTAAGAT GGATTCCTAA TACAAACTGG 
GAAACGAAAC TCCAGAAAAT GCATTTACGC AGGCAGGCAG TAATGACTGC ACTTATCGAG 
GAGCAAAAGA AACGATTTGC TTCAGGAGAG GAACGGAATT GCTTTCTTGA CTTCTTATTA 
TCCGAAGGCT TACTTACAGA GAAACAAATA AGCATGTTGG TTTGGGAGTT AATAATTGAG 
GCATCAGATA CTACTCTGGT CTCCACAGAA TGGGCAATAT ACGAACTTGC AAAGAATCCG 
AAGTACCAGG ATCTGCTGTT TCACGAAATC CAAAATGTAT GTGGATCTGA AAAGATCAAG 
GAGGAACACT TGTCTCAATT GCCATTCTTG AATGCTGTTT TCCATGAAAC TATCAGGAAG 
TACAGCCCGG CTCCAGTTAT ACCTCTGCGA TATGTGCATG AAGATACAGA AATAGGGAGT 
TACTACATTC CTGCTGGAAG TGAGATTGCT ATTAATATTT ACGGATGCAA CATGGACAAA 
AAGCACTGGG AGAATCCTGA AGAGTGGAAG CCAGAGAGAT TTCTTAATGG AAAATACGAT 
CTTGCTGAGT TACACAAAAC GATGGCTTTT GGAGCTGGAA AGAGAGCATG TGCAGGTGCG 
CTTCAAGCAT CGCTGATAGG AAGTACATCA ATTGGCAGAC TAGTACAAGA TTTTGAATGG 
AGACTGCAAG ATGGGGAGGA AGAAAACGTG GACATTGTTG GACTCACCAC TCGCAAGCTT 
CAGCCTTTGC ACGCCACCAT AAAGCCAAGA AAAACTATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012071727.1
CDS	594..1835
Translation	MLQHAMVTRYSSISTRKLSKALSVLTQDKCMVATSDYDEFHKNAKRCILTNVLGPNAQRRHRPHRDTLVENISTRFHAHLKSYPEQAVNFREVFESELFGVSLKEAFGEDVQSVYVDDLRTTLTREEIFEILVLDPMQGAIDVDWRDFFPYLRWIPNTNWETKLQKMHLRRQAVMTALIEEQKKRFASGEERNCFLDFLLSEGLLTEKQISMLVWELIIEASDTTLVSTEWAIYELAKNPKYQDLLFHEIQNVCGSEKIKEEHLSQLPFLNAVFHETIRKYSPAPVIPLRYVHEDTEIGSYYIPAGSEIAINIYGCNMDKKHWENPEEWKPERFLNGKYDLAELHKTMAFGAGKRACAGALQASLIGSTSIGRLVQDFEWRLQDGEEENVDIVGLTTRKLQPLHATIKPRKTI

Target ORF information:

RefSeq Version	XM_012216337.2
Organism	Jatropha curcas
Definition	Jatropha curcas ent-kaurene oxidase, chloroplastic (EKO), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012216337.2

ORF Insert Sequence:

ATGCTACAAC ATGCCATGGT GACTAGATAT TCATCCATCT CAACAAGGAA ACTATCAAAA 
GCCTTGTCTG TGCTTACCCA GGATAAATGT ATGGTTGCTA CAAGTGATTA TGATGAGTTT 
CACAAGAATG CAAAACGCTG TATACTTACG AATGTATTGG GACCAAATGC CCAGAGGCGA 
CATCGACCCC ACAGGGACAC CCTGGTTGAA AATATTTCAA CCCGGTTTCA TGCCCATTTA 
AAGAGCTATC CTGAACAAGC AGTAAATTTC AGAGAAGTAT TTGAGTCTGA ACTTTTTGGG 
GTATCACTGA AAGAAGCATT TGGAGAGGAT GTGCAATCTG TTTATGTTGA CGACTTGAGG 
ACCACTTTAA CAAGAGAGGA GATTTTCGAA ATTTTAGTGC TTGATCCCAT GCAGGGTGCA 
ATTGATGTGG ACTGGAGAGA TTTTTTCCCA TATTTAAGAT GGATTCCTAA TACAAACTGG 
GAAACGAAAC TCCAGAAAAT GCATTTACGC AGGCAGGCAG TAATGACTGC ACTTATCGAG 
GAGCAAAAGA AACGATTTGC TTCAGGAGAG GAACGGAATT GCTTTCTTGA CTTCTTATTA 
TCCGAAGGCT TACTTACAGA GAAACAAATA AGCATGTTGG TTTGGGAGTT AATAATTGAG 
GCATCAGATA CTACTCTGGT CTCCACAGAA TGGGCAATAT ACGAACTTGC AAAGAATCCG 
AAGTACCAGG ATCTGCTGTT TCACGAAATC CAAAATGTAT GTGGATCTGA AAAGATCAAG 
GAGGAACACT TGTCTCAATT GCCATTCTTG AATGCTGTTT TCCATGAAAC TATCAGGAAG 
TACAGCCCGG CTCCAGTTAT ACCTCTGCGA TATGTGCATG AAGATACAGA AATAGGGAGT 
TACTACATTC CTGCTGGAAG TGAGATTGCT ATTAATATTT ACGGATGCAA CATGGACAAA 
AAGCACTGGG AGAATCCTGA AGAGTGGAAG CCAGAGAGAT TTCTTAATGG AAAATACGAT 
CTTGCTGAGT TACACAAAAC GATGGCTTTT GGAGCTGGAA AGAGAGCATG TGCAGGTGCG 
CTTCAAGCAT CGCTGATAGG AAGTACATCA ATTGGCAGAC TAGTACAAGA TTTTGAATGG 
AGACTGCAAG ATGGGGAGGA AGAAAACGTG GACATTGTTG GACTCACCAC TCGCAAGCTT 
CAGCCTTTGC ACGCCACCAT AAAGCCAAGA AAAACTATAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EKO ( NM_001306026.1 ) cDNA ORF clone, Jatropha curcas -> NP_001292955.1 Jatropha curcas ent-kaurene oxidase, chloroplastic (EKO), mRNA.

Price & Availability↑

CloneID	OJa80471
Clone ID Related Accession (Same CDS sequence)	NM_001306026.1
Accession Version	NM_001306026.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1545bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-08
Organism	Jatropha curcas
Product	ent-kaurene oxidase, chloroplastic
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from JF929910.1. On Apr 10, 2015 this sequence version replaced XM_012216336.1. ##Evidence-Data-START## Transcript exon combination :: JF929910.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2342024, SAMN00003846 [ECO:0000350] ##Evidence-Data-END##

ATGGATGTTA TAACAACAAC TATCCTCCCA AGTTTTCAAG CAATGCCGTA TGCAACTCCC 
GTGGCTGTCG GTGGTTTGTT CTTCTCCATG TTCTTTATGA AGAAATTGAT CAATGATCAA 
AAGAAAAGAA ATTCTAAACT TCCTTCTGTG CCAGAGGTAC CAGGATTGCC AGTGATAGGG 
AATTTGCTGC AACTGAAAGA GAAGAAACCC CACAAGACTT TTGCCAGATG GGCTGAGACT 
TATGGTCCTA TTTATTCAAT TAGAACTGGT GCTTCTACGG TTATTGTTAT CAATTCAGCT 
GATGCTGCAA AAGAGGCCAT GGTGACTAGA TATTCATCCA TCTCAACAAG GAAACTATCA 
AAAGCCTTGT CTGTGCTTAC CCAGGATAAA TGTATGGTTG CTACAAGTGA TTATGATGAG 
TTTCACAAGA ATGCAAAACG CTGTATACTT ACGAATGTAT TGGGACCAAA TGCCCAGAGG 
CGACATCGAC CCCACAGGGA CACCCTGGTT GAAAATATTT CAACCCGGTT TCATGCCCAT 
TTAAAGAGCT ATCCTGAACA AGCAGTAAAT TTCAGAGAAG TATTTGAGTC TGAACTTTTT 
GGGGTATCAC TGAAAGAAGC ATTTGGGGAG GATGTGCAAT CTGTTTATGT TGACGACTTG 
AGGACCACTT TAACAAGAGA GGAGATTTTC GAAATTTTAG TGCTTGATCC CATGCAGGGT 
GCAATTGATG TGGACTGGAG AGATTTTTTC CCATATTTAA GATGGATTCC TAATACAAAC 
TGGGAAACGA AACTCCAGAA AATGCATTTA CGCAGGCAGG CAGTAATGAC TGCACTTATC 
GAGGAGCAAA AGAAACGATT TGCTTCAGGA GAGGAACGGA ATTGCTTTCT TGACTTCTTA 
TTATCCGAAG GCTTACTTAC AGAGAAACAA ATAAGCATGT TGGTTTGGGA GTTAATAATT 
GAGGCATCAG ATACTACTCT GGTCTCCACA GAATGGGCAA TATACGAACT TGCAAAGAAT 
CCGAAGTACC AGGATCTGCT GTTTCACGAA ATCCAAAATG TATGTGGATC TGAAAAGATC 
AAGGAGGAGC ACTTGTCTCA ACTGCCATTC TTGAATGCTG TTTTCCATGA AACTATCAGG 
AAGTACAGCC CGGCTCCAGT TATACCTCTG CGATATGTGC ATGAAGATAC AGAACTGGGC 
GGTTACTACA TTCCTGCTGG AAGTGAGATT GCTATTAATA TTTACGGATG CAACATGGAC 
AAAAAGCACT GGGAGAATCC TGAAGAGTGG AAGCCAGAGA GATTTCTTAA TGGAAAATAC 
GATCTTGCTG AGTTACACAA AACGATGGCT TTTGGAGCTG GAAAGAGAGC ATGTGCAGGT 
GCGCTTCAAG CATCGCTGAT AGGAAGTACA TCAATTGGCA GACTAGTACA AGATTTTGAA 
TGGAGACTGC AAGATGGGGA GGAAGAAAAC GTGGACACTG TTGGACTCAC CACTCGCAAG 
CTTCAGCCTT TGCACGCCAC CATAAAGCCA AGAAAAACTA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292955.1
CDS	1..1545
Translation	MDVITTTILPSFQAMPYATPVAVGGLFFSMFFMKKLINDQKKRNSKLPSVPEVPGLPVIGNLLQLKEKKPHKTFARWAETYGPIYSIRTGASTVIVINSADAAKEAMVTRYSSISTRKLSKALSVLTQDKCMVATSDYDEFHKNAKRCILTNVLGPNAQRRHRPHRDTLVENISTRFHAHLKSYPEQAVNFREVFESELFGVSLKEAFGEDVQSVYVDDLRTTLTREEIFEILVLDPMQGAIDVDWRDFFPYLRWIPNTNWETKLQKMHLRRQAVMTALIEEQKKRFASGEERNCFLDFLLSEGLLTEKQISMLVWELIIEASDTTLVSTEWAIYELAKNPKYQDLLFHEIQNVCGSEKIKEEHLSQLPFLNAVFHETIRKYSPAPVIPLRYVHEDTELGGYYIPAGSEIAINIYGCNMDKKHWENPEEWKPERFLNGKYDLAELHKTMAFGAGKRACAGALQASLIGSTSIGRLVQDFEWRLQDGEEENVDTVGLTTRKLQPLHATIKPRKTI

Target ORF information:

RefSeq Version	NM_001306026.1
Organism	Jatropha curcas
Definition	Jatropha curcas ent-kaurene oxidase, chloroplastic (EKO), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001306026.1

ORF Insert Sequence:

ATGGATGTTA TAACAACAAC TATCCTCCCA AGTTTTCAAG CAATGCCGTA TGCAACTCCC 
GTGGCTGTCG GTGGTTTGTT CTTCTCCATG TTCTTTATGA AGAAATTGAT CAATGATCAA 
AAGAAAAGAA ATTCTAAACT TCCTTCTGTG CCAGAGGTAC CAGGATTGCC AGTGATAGGG 
AATTTGCTGC AACTGAAAGA GAAGAAACCC CACAAGACTT TTGCCAGATG GGCTGAGACT 
TATGGTCCTA TTTATTCAAT TAGAACTGGT GCTTCTACGG TTATTGTTAT CAATTCAGCT 
GATGCTGCAA AAGAGGCCAT GGTGACTAGA TATTCATCCA TCTCAACAAG GAAACTATCA 
AAAGCCTTGT CTGTGCTTAC CCAGGATAAA TGTATGGTTG CTACAAGTGA TTATGATGAG 
TTTCACAAGA ATGCAAAACG CTGTATACTT ACGAATGTAT TGGGACCAAA TGCCCAGAGG 
CGACATCGAC CCCACAGGGA CACCCTGGTT GAAAATATTT CAACCCGGTT TCATGCCCAT 
TTAAAGAGCT ATCCTGAACA AGCAGTAAAT TTCAGAGAAG TATTTGAGTC TGAACTTTTT 
GGGGTATCAC TGAAAGAAGC ATTTGGGGAG GATGTGCAAT CTGTTTATGT TGACGACTTG 
AGGACCACTT TAACAAGAGA GGAGATTTTC GAAATTTTAG TGCTTGATCC CATGCAGGGT 
GCAATTGATG TGGACTGGAG AGATTTTTTC CCATATTTAA GATGGATTCC TAATACAAAC 
TGGGAAACGA AACTCCAGAA AATGCATTTA CGCAGGCAGG CAGTAATGAC TGCACTTATC 
GAGGAGCAAA AGAAACGATT TGCTTCAGGA GAGGAACGGA ATTGCTTTCT TGACTTCTTA 
TTATCCGAAG GCTTACTTAC AGAGAAACAA ATAAGCATGT TGGTTTGGGA GTTAATAATT 
GAGGCATCAG ATACTACTCT GGTCTCCACA GAATGGGCAA TATACGAACT TGCAAAGAAT 
CCGAAGTACC AGGATCTGCT GTTTCACGAA ATCCAAAATG TATGTGGATC TGAAAAGATC 
AAGGAGGAGC ACTTGTCTCA ACTGCCATTC TTGAATGCTG TTTTCCATGA AACTATCAGG 
AAGTACAGCC CGGCTCCAGT TATACCTCTG CGATATGTGC ATGAAGATAC AGAACTGGGC 
GGTTACTACA TTCCTGCTGG AAGTGAGATT GCTATTAATA TTTACGGATG CAACATGGAC 
AAAAAGCACT GGGAGAATCC TGAAGAGTGG AAGCCAGAGA GATTTCTTAA TGGAAAATAC 
GATCTTGCTG AGTTACACAA AACGATGGCT TTTGGAGCTG GAAAGAGAGC ATGTGCAGGT 
GCGCTTCAAG CATCGCTGAT AGGAAGTACA TCAATTGGCA GACTAGTACA AGATTTTGAA 
TGGAGACTGC AAGATGGGGA GGAAGAAAAC GTGGACACTG TTGGACTCAC CACTCGCAAG 
CTTCAGCCTT TGCACGCCAC CATAAAGCCA AGAAAAACTA TATGA