crim1 cDNA ORF clone, Ictalurus punctatus(channel catfish)

Gene
Clones
gRNAs

Summary

Gene Symbol	crim1
Entrez Gene ID	108270060
Full Name	cysteine rich transmembrane BMP regulator 1
Gene Type	protein-coding
Organism	Ictalurus punctatus(channel catfish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017476348.1	XP_017331837.1	cysteine-rich motor neuron 1 protein isoform X1
XM_017476349.1	XP_017331838.1	cysteine-rich motor neuron 1 protein isoform X2

Pathways from BioSystems
Gene Ontology

The following crim1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crim1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OIc38107	XM_017476348.1 Latest version!	Ictalurus punctatus cysteine rich transmembrane BMP regulator 1 (crim1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OIc38108	XM_017476349.1 Latest version!	Ictalurus punctatus cysteine rich transmembrane BMP regulator 1 (crim1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

crim1 ( XM_017476348.1 ) cDNA ORF clone, Ictalurus punctatus(channel catfish) -> XP_017331837.1 Ictalurus punctatus cysteine rich transmembrane BMP regulator 1 (crim1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OIc38107
Clone ID Related Accession (Same CDS sequence)	XM_017476348.1
Accession Version	XM_017476348.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-05
Organism	Ictalurus punctatus(channel catfish)
Product	cysteine-rich motor neuron 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030424.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictalurus punctatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTCTT CATCATCGTT CCTCACCTTG AGGATGCACG TTCTTCTGAA CTGCGTGTTG 
ATCTCGCAGC TCGCGCTGCT GCTGCTGCTT GCGAGAAGTG GCCGGGCGCT GTTGTGTTTG 
CCCTGCGACG AGTCCAAGTG CGAGGAGCCG AAGCAGTGTG TCGGCGCCGT GGTGCCTGGT 
ATATGCGGCT GCTGCTCCGT GTGCGCCAAG CAGAAGAACG AGAGCTGCGG CGGTGTGTAC 
GGCCTGTACG GCACGTGCGA CGCGGGGCTG CGGTGCGTGA TCAGACCTCC GCTCGACGGC 
GGCTCCATCA CGCAGTACGA AGTCGGAGTT TGCGAAGATG AAAACTGGGA TGACGACCAG 
CTCTTGGGAT TTGAACCGTG CAACGAGAAC TTGGTTACAG GCTGTAACAT AATTGATGGG 
AGGTGTGAAT GTGACAGCGT ACGAACATGC AACAACCCAT TTGAGTTTGC CAGCGAGGAG 
GCTTGTCAGA CAGCCCTGAA GAAGATCGAA GAGGAGAGGC CGGACTGTTC TAAAGCCAGG 
TGTGAGGTAC AGTTCTCTCC CCGTTGTCCT GAGGACTCAG TGCTGATTGA AGGTTACGCT 
CCACCTGGCG AATGCTGCCC TCTGCCCAGT CGCTGTGTGT GCAGTCCTTC TGGGTGTCTG 
AGAAAAGTAT GCCAACCTGG CTATCTCAAC ATCCTCGTAT CCAAAAGCTC TGGCAAACCT 
GGAGAATGCT GCGACCTGTA CGAGTGCAAA CCAGTTTTCA GTGTGGACTG CAGCACGGTG 
GAATGTCCTC CCATACAGCC CATGCAGTGT CCAGCAGACA GCTACGAAAC GCAGGTCAGG 
CTCACAGCCG ACGGATGCTG CACACTACCC ACCAGGTGTG AGTGTTTGCC TGGTGTCTGC 
ACCTTCCCAC AGTGCTCAGC AGGAACACAG CCCCACTTGG TCTCCCGTGG TGATGGAACA 
CCGGGACGGT GCTGTGATGT GTACGAGTGT GTCAATGAGA CTAAGCCAGC CTGTATGTTC 
AATGGAGTGG AGTATCATGA TGGTGACATG TTCAGGATGG ATGCCTGTCA TTTCTGCCGC 
TGCCAGGGCG GTGTATCAGT GTGCTTCACG GCACAGTGCG GTGTCCTCCA CTGCGAACGC 
TACTATGTAC CAGAGGGAGA GTGCTGTCCT GTCTGTGAAG ATCCTATCTT CCCTGCGCTG 
AATTTTGCCG GCTGCTATGT GAATGGCCAG ATCCTGGCAC ATGGGGATCA CTGGCGAGAG 
GACGACTGCA CATTCTGCCA GTGTGTGAGT GGCGAGGCCC GCTGTGTAGC TGCTGCCTGC 
GGCCATAACT GCCTCCATCC GGTCACAGTG CCTGGAGAAT GCTGTCCTGT GTGTGAGGAG 
CCCACCTATA TCACCATGGC GCCCCCTGGC TGCAGCTTGC TGGAGAACTG CACGCTGACG 
GAGAAAGATT GTGCCTTCGG CTTCCGGCTC AACTCGCACG GATGCCGCAC CTGCAGCTGC 
AAAACAAGAG AGGAGCTGTG CAGTGCTCTT ATCTCGGGCT GCTCCTTGAA GTGTTCACTC 
GGCTTCCAGA TAGACAGCAA TGGCTGTCCC ATGTGTCAGT GCCGGCATCG CCACAAGAAG 
TGCAAGCCAG TAGCCTGCGC CAAGTACTGC CCCTTCGGCT TTGTTAAAAA CAAGCATGGC 
TGTGATACCT GTCGCTGTAA AAAATGCCCT GAACTGCCAT GTGACAAATC CTGTCCAGTG 
GGCTTCCAGC AGGATGAGCA GGGTTGTCTG ATTTGCCAGT GCAGAGACCA GGGTTCGTCT 
TCTCTTGGCC CCTCCGTTAA GCTGGGCTCA TGTCTCTCTA TGAATGGTCG GAGACATGAG 
AATGGCCAAA GCTGGCACGA TGGCTGCAGA GAGTGCTACT GCCACAATGG CCGAGAGATG 
TGTGCCCTAA TATCCTGCCC TGTGCCTAAC TGTGACAACC CGAGCATCCG CGCCAGCCAG 
TGCTGTCCCT CCTGCCCAGA TGAGTCTGGC TCTCACAGGC CAGAGCTGAG CGAGGCGTCT 
GTGTGTCTCG CCCCGGGTGG AGAGTACTTT GTGGAAGGAG AGACGTGGAA CATTGACTCA 
TGTACACAGT GCACCTGCCA CAGCGGCCGC GTGTTGTGCG AGACCGAGGT GTGTCCTCCT 
CTGCTCTGCC AGAGCCCAAT CAGAACACAG GACTCCTGCT GCCCACACTG CCCAGATGAA 
CTGCTCACTC CGCCGGCCCC CAGCAACAAC AGCATGCCCA GCTACTGCCG CAACGAGGAG 
GGAGACATCT TTCTGGCAGC CGAGTCCTGG AAGCCAAACG TTTGCTCCAG CTGTGTGTGT 
GTGGATGGAG TCATCAGCTG TTTCTCAGAA TCCTGCCCTC CGGTCAACTG TGCCCGGCCT 
GTCCTGCGCA AGGGCCAGTG CTGCCCCTAT TGTCTAGATG CCACACCGAG AGCTGTGTGC 
CACTTCAATG GGAAAACATA CGTGGATGAG GAGCGCTGGG ACATTGACAG CTGCACACAT 
TGCTACTGCT TGCAAGGCCA GACGCTTTGC TCCACAGTCA GTTGCCCTGC CCTCCCCTGC 
CACCAGCCAA TCACAGTGGA GGGCAGCTGC TGTCCCTTGT GCCCAGAGAT TGTCCCCACT 
AATGTGCCTA TAGAGAAGAC AGACCAGCAT GGAGACAGAC TGAAACAACG ACCTGCTTGG 
CCTACTCACA GTGAGAACGA CATCATGCCC CAGTTCAGAG CAGGTAACTT TGGCAGCCTG 
CAGATGCCGT ATCTAGATGG GAAGACACCG ATGCTCAGTG AGGATGCCAG CCTTCATTGG 
GCCTGGGTAG CATTGCCCAT CCTCATGATG GTGCTCATTA TCACCACTCT GCTGCTAGTC 
AACCAGAGGA AGCAGTGGAT GCATGTACCT TGCTACCACG CACCCTCTAA GCCCACATGT 
CTGAATAACC AACTGGTGTA CGTTGACTGC CAGAAAGGGA CGAAGGTACA AGTGGACAGC 
TCCCAGCGCA TGCTGCGCAT CGCCGACCCT GACTCACGCT ACAGTGGTTA TTACAGCATG 
CAGAAACATA ACAACCTGGA GGCAGACAAC TTCTACCAGA CTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017331837.1
CDS	624..3731
Translation	MVSSSSFLTLRMHVLLNCVLISQLALLLLLARSGRALLCLPCDESKCEEPKQCVGAVVPGICGCCSVCAKQKNESCGGVYGLYGTCDAGLRCVIRPPLDGGSITQYEVGVCEDENWDDDQLLGFEPCNENLVTGCNIIDGRCECDSVRTCNNPFEFASEEACQTALKKIEEERPDCSKARCEVQFSPRCPEDSVLIEGYAPPGECCPLPSRCVCSPSGCLRKVCQPGYLNILVSKSSGKPGECCDLYECKPVFSVDCSTVECPPIQPMQCPADSYETQVRLTADGCCTLPTRCECLPGVCTFPQCSAGTQPHLVSRGDGTPGRCCDVYECVNETKPACMFNGVEYHDGDMFRMDACHFCRCQGGVSVCFTAQCGVLHCERYYVPEGECCPVCEDPIFPALNFAGCYVNGQILAHGDHWREDDCTFCQCVSGEARCVAAACGHNCLHPVTVPGECCPVCEEPTYITMAPPGCSLLENCTLTEKDCAFGFRLNSHGCRTCSCKTREELCSALISGCSLKCSLGFQIDSNGCPMCQCRHRHKKCKPVACAKYCPFGFVKNKHGCDTCRCKKCPELPCDKSCPVGFQQDEQGCLICQCRDQGSSSLGPSVKLGSCLSMNGRRHENGQSWHDGCRECYCHNGREMCALISCPVPNCDNPSIRASQCCPSCPDESGSHRPELSEASVCLAPGGEYFVEGETWNIDSCTQCTCHSGRVLCETEVCPPLLCQSPIRTQDSCCPHCPDELLTPPAPSNNSMPSYCRNEEGDIFLAAESWKPNVCSSCVCVDGVISCFSESCPPVNCARPVLRKGQCCPYCLDATPRAVCHFNGKTYVDEERWDIDSCTHCYCLQGQTLCSTVSCPALPCHQPITVEGSCCPLCPEIVPTNVPIEKTDQHGDRLKQRPAWPTHSENDIMPQFRAGNFGSLQMPYLDGKTPMLSEDASLHWAWVALPILMMVLIITTLLLVNQRKQWMHVPCYHAPSKPTCLNNQLVYVDCQKGTKVQVDSSQRMLRIADPDSRYSGYYSMQKHNNLEADNFYQTA

Target ORF information:

RefSeq Version	XM_017476348.1
Organism	Ictalurus punctatus(channel catfish)
Definition	Ictalurus punctatus cysteine rich transmembrane BMP regulator 1 (crim1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017476348.1

ORF Insert Sequence:

ATGGTCTCTT CATCATCGTT CCTCACCTTG AGGATGCACG TTCTTCTGAA CTGCGTGTTG 
ATCTCGCAGC TCGCGCTGCT GCTGCTGCTT GCGAGAAGTG GCCGGGCGCT GTTGTGTTTG 
CCCTGCGACG AGTCCAAGTG CGAGGAGCCG AAGCAGTGTG TCGGCGCCGT GGTGCCTGGT 
ATATGCGGCT GCTGCTCCGT GTGCGCCAAG CAGAAGAACG AGAGCTGCGG CGGTGTGTAC 
GGCCTGTACG GCACGTGCGA CGCGGGGCTG CGGTGCGTGA TCAGACCTCC GCTCGACGGC 
GGCTCCATCA CGCAGTACGA AGTCGGAGTT TGCGAAGATG AAAACTGGGA TGACGACCAG 
CTCTTGGGAT TTGAACCGTG CAACGAGAAC TTGGTTACAG GCTGTAACAT AATTGATGGG 
AGGTGTGAAT GTGACAGCGT ACGAACATGC AACAACCCAT TTGAGTTTGC CAGCGAGGAG 
GCTTGTCAGA CAGCCCTGAA GAAGATCGAA GAGGAGAGGC CGGACTGTTC TAAAGCCAGG 
TGTGAGGTAC AGTTCTCTCC CCGTTGTCCT GAGGACTCAG TGCTGATTGA AGGTTACGCT 
CCACCTGGCG AATGCTGCCC TCTGCCCAGT CGCTGTGTGT GCAGTCCTTC TGGGTGTCTG 
AGAAAAGTAT GCCAACCTGG CTATCTCAAC ATCCTCGTAT CCAAAAGCTC TGGCAAACCT 
GGAGAATGCT GCGACCTGTA CGAGTGCAAA CCAGTTTTCA GTGTGGACTG CAGCACGGTG 
GAATGTCCTC CCATACAGCC CATGCAGTGT CCAGCAGACA GCTACGAAAC GCAGGTCAGG 
CTCACAGCCG ACGGATGCTG CACACTACCC ACCAGGTGTG AGTGTTTGCC TGGTGTCTGC 
ACCTTCCCAC AGTGCTCAGC AGGAACACAG CCCCACTTGG TCTCCCGTGG TGATGGAACA 
CCGGGACGGT GCTGTGATGT GTACGAGTGT GTCAATGAGA CTAAGCCAGC CTGTATGTTC 
AATGGAGTGG AGTATCATGA TGGTGACATG TTCAGGATGG ATGCCTGTCA TTTCTGCCGC 
TGCCAGGGCG GTGTATCAGT GTGCTTCACG GCACAGTGCG GTGTCCTCCA CTGCGAACGC 
TACTATGTAC CAGAGGGAGA GTGCTGTCCT GTCTGTGAAG ATCCTATCTT CCCTGCGCTG 
AATTTTGCCG GCTGCTATGT GAATGGCCAG ATCCTGGCAC ATGGGGATCA CTGGCGAGAG 
GACGACTGCA CATTCTGCCA GTGTGTGAGT GGCGAGGCCC GCTGTGTAGC TGCTGCCTGC 
GGCCATAACT GCCTCCATCC GGTCACAGTG CCTGGAGAAT GCTGTCCTGT GTGTGAGGAG 
CCCACCTATA TCACCATGGC GCCCCCTGGC TGCAGCTTGC TGGAGAACTG CACGCTGACG 
GAGAAAGATT GTGCCTTCGG CTTCCGGCTC AACTCGCACG GATGCCGCAC CTGCAGCTGC 
AAAACAAGAG AGGAGCTGTG CAGTGCTCTT ATCTCGGGCT GCTCCTTGAA GTGTTCACTC 
GGCTTCCAGA TAGACAGCAA TGGCTGTCCC ATGTGTCAGT GCCGGCATCG CCACAAGAAG 
TGCAAGCCAG TAGCCTGCGC CAAGTACTGC CCCTTCGGCT TTGTTAAAAA CAAGCATGGC 
TGTGATACCT GTCGCTGTAA AAAATGCCCT GAACTGCCAT GTGACAAATC CTGTCCAGTG 
GGCTTCCAGC AGGATGAGCA GGGTTGTCTG ATTTGCCAGT GCAGAGACCA GGGTTCGTCT 
TCTCTTGGCC CCTCCGTTAA GCTGGGCTCA TGTCTCTCTA TGAATGGTCG GAGACATGAG 
AATGGCCAAA GCTGGCACGA TGGCTGCAGA GAGTGCTACT GCCACAATGG CCGAGAGATG 
TGTGCCCTAA TATCCTGCCC TGTGCCTAAC TGTGACAACC CGAGCATCCG CGCCAGCCAG 
TGCTGTCCCT CCTGCCCAGA TGAGTCTGGC TCTCACAGGC CAGAGCTGAG CGAGGCGTCT 
GTGTGTCTCG CCCCGGGTGG AGAGTACTTT GTGGAAGGAG AGACGTGGAA CATTGACTCA 
TGTACACAGT GCACCTGCCA CAGCGGCCGC GTGTTGTGCG AGACCGAGGT GTGTCCTCCT 
CTGCTCTGCC AGAGCCCAAT CAGAACACAG GACTCCTGCT GCCCACACTG CCCAGATGAA 
CTGCTCACTC CGCCGGCCCC CAGCAACAAC AGCATGCCCA GCTACTGCCG CAACGAGGAG 
GGAGACATCT TTCTGGCAGC CGAGTCCTGG AAGCCAAACG TTTGCTCCAG CTGTGTGTGT 
GTGGATGGAG TCATCAGCTG TTTCTCAGAA TCCTGCCCTC CGGTCAACTG TGCCCGGCCT 
GTCCTGCGCA AGGGCCAGTG CTGCCCCTAT TGTCTAGATG CCACACCGAG AGCTGTGTGC 
CACTTCAATG GGAAAACATA CGTGGATGAG GAGCGCTGGG ACATTGACAG CTGCACACAT 
TGCTACTGCT TGCAAGGCCA GACGCTTTGC TCCACAGTCA GTTGCCCTGC CCTCCCCTGC 
CACCAGCCAA TCACAGTGGA GGGCAGCTGC TGTCCCTTGT GCCCAGAGAT TGTCCCCACT 
AATGTGCCTA TAGAGAAGAC AGACCAGCAT GGAGACAGAC TGAAACAACG ACCTGCTTGG 
CCTACTCACA GTGAGAACGA CATCATGCCC CAGTTCAGAG CAGGTAACTT TGGCAGCCTG 
CAGATGCCGT ATCTAGATGG GAAGACACCG ATGCTCAGTG AGGATGCCAG CCTTCATTGG 
GCCTGGGTAG CATTGCCCAT CCTCATGATG GTGCTCATTA TCACCACTCT GCTGCTAGTC 
AACCAGAGGA AGCAGTGGAT GCATGTACCT TGCTACCACG CACCCTCTAA GCCCACATGT 
CTGAATAACC AACTGGTGTA CGTTGACTGC CAGAAAGGGA CGAAGGTACA AGTGGACAGC 
TCCCAGCGCA TGCTGCGCAT CGCCGACCCT GACTCACGCT ACAGTGGTTA TTACAGCATG 
CAGAAACATA ACAACCTGGA GGCAGACAAC TTCTACCAGA CTGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

crim1 ( XM_017476349.1 ) cDNA ORF clone, Ictalurus punctatus(channel catfish) -> XP_017331838.1 Ictalurus punctatus cysteine rich transmembrane BMP regulator 1 (crim1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OIc38108
Clone ID Related Accession (Same CDS sequence)	XM_017476349.1
Accession Version	XM_017476349.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3105bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-05
Organism	Ictalurus punctatus(channel catfish)
Product	cysteine-rich motor neuron 1 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030424.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictalurus punctatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTCTT CATCATCGTT CCTCACCTTG AGGATGCACG TTCTTCTGAA CTGCGTGTTG 
ATCTCGCAGC TCGCGCTGCT GCTGCTGCTT GCGAGAAGTG GCCGGGCGCT GTTGTGTTTG 
CCCTGCGACG AGTCCAAGTG CGAGGAGCCG AAGCAGTGTG TCGGCGCCGT GGTGCCTGGT 
ATATGCGGCT GCTGCTCCGT GTGCGCCAAG CAGAAGAACG AGAGCTGCGG CGGTGTGTAC 
GGCCTGTACG GCACGTGCGA CGCGGGGCTG CGGTGCGTGA TCAGACCTCC GCTCGACGGC 
GGCTCCATCA CGCAGTACGA AGTCGGAGTT TGCGAAGATG AAAACTGGGA TGACGACCAG 
CTCTTGGGAT TTGAACCGTG CAACGAGAAC TTGGTTACAG GCTGTAACAT AATTGATGGG 
AGGTGTGAAT GTGACAGCGT ACGAACATGC AACAACCCAT TTGAGTTTGC CAGCGAGGAG 
GCTTGTCAGA CAGCCCTGAA GAAGATCGAA GAGGAGAGGC CGGACTGTTC TAAAGCCAGG 
TGTGAGGTAC AGTTCTCTCC CCGTTGTCCT GAGGACTCAG TGCTGATTGA AGGTTACGCT 
CCACCTGGCG AATGCTGCCC TCTGCCCAGT CGCTGTGTGT GCAGTCCTTC TGGGTGTCTG 
AGAAAAGTAT GCCAACCTGG CTATCTCAAC ATCCTCGTAT CCAAAAGCTC TGGCAAACCT 
GGAGAATGCT GCGACCTGTA CGAGTGCAAA CCAGTTTTCA GTGTGGACTG CAGCACGGTG 
GAATGTCCTC CCATACAGCC CATGCAGTGT CCAGCAGACA GCTACGAAAC GCAGGTCAGG 
CTCACAGCCG ACGGATGCTG CACACTACCC ACCAGGTGTG AGTGTTTGCC TGGTGTCTGC 
ACCTTCCCAC AGTGCTCAGC AGGAACACAG CCCCACTTGG TCTCCCGTGG TGATGGAACA 
CCGGGACGGT GCTGTGATGT GTACGAGTGT GTCAATGAGA CTAAGCCAGC CTGTATGTTC 
AATGGAGTGG AGTATCATGA TGGTGACATG TTCAGGATGG ATGCCTGTCA TTTCTGCCGC 
TGCCAGGGCG GTGTATCAGT GTGCTTCACG GCACAGTGCG GTGTCCTCCA CTGCGAACGC 
TACTATGTAC CAGAGGGAGA GTGCTGTCCT GTCTGTGAAG ATCCTATCTT CCCTGCGCTG 
AATTTTGCCG GCTGCTATGT GAATGGCCAG ATCCTGGCAC ATGGGGATCA CTGGCGAGAG 
GACGACTGCA CATTCTGCCA GTGTGTGAGT GGCGAGGCCC GCTGTGTAGC TGCTGCCTGC 
GGCCATAACT GCCTCCATCC GGTCACAGTG CCTGGAGAAT GCTGTCCTGT GTGTGAGGAG 
CCCACCTATA TCACCATGGC GCCCCCTGGC TGCAGCTTGC TGGAGAACTG CACGCTGACG 
GAGAAAGATT GTGCCTTCGG CTTCCGGCTC AACTCGCACG GATGCCGCAC CTGCAGCTGC 
AAAACAAGAG AGGAGCTGTG CAGTGCTCTT ATCTCGGGCT GCTCCTTGAA GTGTTCACTC 
GGCTTCCAGA TAGACAGCAA TGGCTGTCCC ATGTGTCAGT GCCGGCATCG CCACAAGAAG 
TGCAAGCCAG TAGCCTGCGC CAAGTACTGC CCCTTCGGCT TTGTTAAAAA CAAGCATGGC 
TGTGATACCT GTCGCTGTAA AAAATGCCCT GAACTGCCAT GTGACAAATC CTGTCCAGTG 
GGCTTCCAGC AGGATGAGCA GGGTTGTCTG ATTTGCCAGT GCAGAGACCA GGGTTCGTCT 
TCTCTTGGCC CCTCCGTTAA GCTGGGCTCA TGTCTCTCTA TGAATGGTCG GAGACATGAG 
AATGGCCAAA GCTGGCACGA TGGCTGCAGA GAGTGCTACT GCCACAATGG CCGAGAGATG 
TGTGCCCTAA TATCCTGCCC TGTGCCTAAC TGTGACAACC CGAGCATCCG CGCCAGCCAG 
TGCTGTCCCT CCTGCCCAGA TGAGTCTGGC TCTCACAGGC CAGAGCTGAG CGAGGCGTCT 
GTGTGTCTCG CCCCGGGTGG AGAGTACTTT GTGGAAGGAG AGACGTGGAA CATTGACTCA 
TGTACACAGT GCACCTGCCA CAGCGGCCGC GTGTTGTGCG AGACCGAGGT GTGTCCTCCT 
CTGCTCTGCC AGAGCCCAAT CAGAACACAG GACTCCTGCT GCCCACACTG CCCAGATGAA 
CTGCTCACTC CGCCGGCCCC CAGCAACAAC AGCATGCCCA GCTACTGCCG CAACGAGGAG 
GGAGACATCT TTCTGGCAGC CGAGTCCTGG AAGCCAAACG TTTGCTCCAG CTGTGTGTGT 
GTGGATGGAG TCATCAGCTG TTTCTCAGAA TCCTGCCCTC CGGTCAACTG TGCCCGGCCT 
GTCCTGCGCA AGGGCCAGTG CTGCCCCTAT TGTCTAGATG CCACACCGAG AGCTGTGTGC 
CACTTCAATG GGAAAACATA CGTGGATGAG GAGCGCTGGG ACATTGACAG CTGCACACAT 
TGCTACTGCT TGCAAGGCCA GACGCTTTGC TCCACAGTCA GTTGCCCTGC CCTCCCCTGC 
CACCAGCCAA TCACAGTGGA GGGCAGCTGC TGTCCCTTGT GCCCAGAGAT TGTCCCCACT 
AATGTGCCTA TAGAGAAGAC AGACCAGCAT GGAGACAGAC TGAAACAACG ACCTGCTTGG 
CCTACTCACA GTGAGAACGA CATCATGCCC CAGTTCAGAG GTAACTTTGG CAGCCTGCAG 
ATGCCGTATC TAGATGGGAA GACACCGATG CTCAGTGAGG ATGCCAGCCT TCATTGGGCC 
TGGGTAGCAT TGCCCATCCT CATGATGGTG CTCATTATCA CCACTCTGCT GCTAGTCAAC 
CAGAGGAAGC AGTGGATGCA TGTACCTTGC TACCACGCAC CCTCTAAGCC CACATGTCTG 
AATAACCAAC TGGTGTACGT TGACTGCCAG AAAGGGACGA AGGTACAAGT GGACAGCTCC 
CAGCGCATGC TGCGCATCGC CGACCCTGAC TCACGCTACA GTGGTTATTA CAGCATGCAG 
AAACATAACA ACCTGGAGGC AGACAACTTC TACCAGACTG CGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017331838.1
CDS	624..3728
Translation	MVSSSSFLTLRMHVLLNCVLISQLALLLLLARSGRALLCLPCDESKCEEPKQCVGAVVPGICGCCSVCAKQKNESCGGVYGLYGTCDAGLRCVIRPPLDGGSITQYEVGVCEDENWDDDQLLGFEPCNENLVTGCNIIDGRCECDSVRTCNNPFEFASEEACQTALKKIEEERPDCSKARCEVQFSPRCPEDSVLIEGYAPPGECCPLPSRCVCSPSGCLRKVCQPGYLNILVSKSSGKPGECCDLYECKPVFSVDCSTVECPPIQPMQCPADSYETQVRLTADGCCTLPTRCECLPGVCTFPQCSAGTQPHLVSRGDGTPGRCCDVYECVNETKPACMFNGVEYHDGDMFRMDACHFCRCQGGVSVCFTAQCGVLHCERYYVPEGECCPVCEDPIFPALNFAGCYVNGQILAHGDHWREDDCTFCQCVSGEARCVAAACGHNCLHPVTVPGECCPVCEEPTYITMAPPGCSLLENCTLTEKDCAFGFRLNSHGCRTCSCKTREELCSALISGCSLKCSLGFQIDSNGCPMCQCRHRHKKCKPVACAKYCPFGFVKNKHGCDTCRCKKCPELPCDKSCPVGFQQDEQGCLICQCRDQGSSSLGPSVKLGSCLSMNGRRHENGQSWHDGCRECYCHNGREMCALISCPVPNCDNPSIRASQCCPSCPDESGSHRPELSEASVCLAPGGEYFVEGETWNIDSCTQCTCHSGRVLCETEVCPPLLCQSPIRTQDSCCPHCPDELLTPPAPSNNSMPSYCRNEEGDIFLAAESWKPNVCSSCVCVDGVISCFSESCPPVNCARPVLRKGQCCPYCLDATPRAVCHFNGKTYVDEERWDIDSCTHCYCLQGQTLCSTVSCPALPCHQPITVEGSCCPLCPEIVPTNVPIEKTDQHGDRLKQRPAWPTHSENDIMPQFRGNFGSLQMPYLDGKTPMLSEDASLHWAWVALPILMMVLIITTLLLVNQRKQWMHVPCYHAPSKPTCLNNQLVYVDCQKGTKVQVDSSQRMLRIADPDSRYSGYYSMQKHNNLEADNFYQTA

Target ORF information:

RefSeq Version	XM_017476349.1
Organism	Ictalurus punctatus(channel catfish)
Definition	Ictalurus punctatus cysteine rich transmembrane BMP regulator 1 (crim1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017476349.1

ORF Insert Sequence:

ATGGTCTCTT CATCATCGTT CCTCACCTTG AGGATGCACG TTCTTCTGAA CTGCGTGTTG 
ATCTCGCAGC TCGCGCTGCT GCTGCTGCTT GCGAGAAGTG GCCGGGCGCT GTTGTGTTTG 
CCCTGCGACG AGTCCAAGTG CGAGGAGCCG AAGCAGTGTG TCGGCGCCGT GGTGCCTGGT 
ATATGCGGCT GCTGCTCCGT GTGCGCCAAG CAGAAGAACG AGAGCTGCGG CGGTGTGTAC 
GGCCTGTACG GCACGTGCGA CGCGGGGCTG CGGTGCGTGA TCAGACCTCC GCTCGACGGC 
GGCTCCATCA CGCAGTACGA AGTCGGAGTT TGCGAAGATG AAAACTGGGA TGACGACCAG 
CTCTTGGGAT TTGAACCGTG CAACGAGAAC TTGGTTACAG GCTGTAACAT AATTGATGGG 
AGGTGTGAAT GTGACAGCGT ACGAACATGC AACAACCCAT TTGAGTTTGC CAGCGAGGAG 
GCTTGTCAGA CAGCCCTGAA GAAGATCGAA GAGGAGAGGC CGGACTGTTC TAAAGCCAGG 
TGTGAGGTAC AGTTCTCTCC CCGTTGTCCT GAGGACTCAG TGCTGATTGA AGGTTACGCT 
CCACCTGGCG AATGCTGCCC TCTGCCCAGT CGCTGTGTGT GCAGTCCTTC TGGGTGTCTG 
AGAAAAGTAT GCCAACCTGG CTATCTCAAC ATCCTCGTAT CCAAAAGCTC TGGCAAACCT 
GGAGAATGCT GCGACCTGTA CGAGTGCAAA CCAGTTTTCA GTGTGGACTG CAGCACGGTG 
GAATGTCCTC CCATACAGCC CATGCAGTGT CCAGCAGACA GCTACGAAAC GCAGGTCAGG 
CTCACAGCCG ACGGATGCTG CACACTACCC ACCAGGTGTG AGTGTTTGCC TGGTGTCTGC 
ACCTTCCCAC AGTGCTCAGC AGGAACACAG CCCCACTTGG TCTCCCGTGG TGATGGAACA 
CCGGGACGGT GCTGTGATGT GTACGAGTGT GTCAATGAGA CTAAGCCAGC CTGTATGTTC 
AATGGAGTGG AGTATCATGA TGGTGACATG TTCAGGATGG ATGCCTGTCA TTTCTGCCGC 
TGCCAGGGCG GTGTATCAGT GTGCTTCACG GCACAGTGCG GTGTCCTCCA CTGCGAACGC 
TACTATGTAC CAGAGGGAGA GTGCTGTCCT GTCTGTGAAG ATCCTATCTT CCCTGCGCTG 
AATTTTGCCG GCTGCTATGT GAATGGCCAG ATCCTGGCAC ATGGGGATCA CTGGCGAGAG 
GACGACTGCA CATTCTGCCA GTGTGTGAGT GGCGAGGCCC GCTGTGTAGC TGCTGCCTGC 
GGCCATAACT GCCTCCATCC GGTCACAGTG CCTGGAGAAT GCTGTCCTGT GTGTGAGGAG 
CCCACCTATA TCACCATGGC GCCCCCTGGC TGCAGCTTGC TGGAGAACTG CACGCTGACG 
GAGAAAGATT GTGCCTTCGG CTTCCGGCTC AACTCGCACG GATGCCGCAC CTGCAGCTGC 
AAAACAAGAG AGGAGCTGTG CAGTGCTCTT ATCTCGGGCT GCTCCTTGAA GTGTTCACTC 
GGCTTCCAGA TAGACAGCAA TGGCTGTCCC ATGTGTCAGT GCCGGCATCG CCACAAGAAG 
TGCAAGCCAG TAGCCTGCGC CAAGTACTGC CCCTTCGGCT TTGTTAAAAA CAAGCATGGC 
TGTGATACCT GTCGCTGTAA AAAATGCCCT GAACTGCCAT GTGACAAATC CTGTCCAGTG 
GGCTTCCAGC AGGATGAGCA GGGTTGTCTG ATTTGCCAGT GCAGAGACCA GGGTTCGTCT 
TCTCTTGGCC CCTCCGTTAA GCTGGGCTCA TGTCTCTCTA TGAATGGTCG GAGACATGAG 
AATGGCCAAA GCTGGCACGA TGGCTGCAGA GAGTGCTACT GCCACAATGG CCGAGAGATG 
TGTGCCCTAA TATCCTGCCC TGTGCCTAAC TGTGACAACC CGAGCATCCG CGCCAGCCAG 
TGCTGTCCCT CCTGCCCAGA TGAGTCTGGC TCTCACAGGC CAGAGCTGAG CGAGGCGTCT 
GTGTGTCTCG CCCCGGGTGG AGAGTACTTT GTGGAAGGAG AGACGTGGAA CATTGACTCA 
TGTACACAGT GCACCTGCCA CAGCGGCCGC GTGTTGTGCG AGACCGAGGT GTGTCCTCCT 
CTGCTCTGCC AGAGCCCAAT CAGAACACAG GACTCCTGCT GCCCACACTG CCCAGATGAA 
CTGCTCACTC CGCCGGCCCC CAGCAACAAC AGCATGCCCA GCTACTGCCG CAACGAGGAG 
GGAGACATCT TTCTGGCAGC CGAGTCCTGG AAGCCAAACG TTTGCTCCAG CTGTGTGTGT 
GTGGATGGAG TCATCAGCTG TTTCTCAGAA TCCTGCCCTC CGGTCAACTG TGCCCGGCCT 
GTCCTGCGCA AGGGCCAGTG CTGCCCCTAT TGTCTAGATG CCACACCGAG AGCTGTGTGC 
CACTTCAATG GGAAAACATA CGTGGATGAG GAGCGCTGGG ACATTGACAG CTGCACACAT 
TGCTACTGCT TGCAAGGCCA GACGCTTTGC TCCACAGTCA GTTGCCCTGC CCTCCCCTGC 
CACCAGCCAA TCACAGTGGA GGGCAGCTGC TGTCCCTTGT GCCCAGAGAT TGTCCCCACT 
AATGTGCCTA TAGAGAAGAC AGACCAGCAT GGAGACAGAC TGAAACAACG ACCTGCTTGG 
CCTACTCACA GTGAGAACGA CATCATGCCC CAGTTCAGAG GTAACTTTGG CAGCCTGCAG 
ATGCCGTATC TAGATGGGAA GACACCGATG CTCAGTGAGG ATGCCAGCCT TCATTGGGCC 
TGGGTAGCAT TGCCCATCCT CATGATGGTG CTCATTATCA CCACTCTGCT GCTAGTCAAC 
CAGAGGAAGC AGTGGATGCA TGTACCTTGC TACCACGCAC CCTCTAAGCC CACATGTCTG 
AATAACCAAC TGGTGTACGT TGACTGCCAG AAAGGGACGA AGGTACAAGT GGACAGCTCC 
CAGCGCATGC TGCGCATCGC CGACCCTGAC TCACGCTACA GTGGTTATTA CAGCATGCAG 
AAACATAACA ACCTGGAGGC AGACAACTTC TACCAGACTG CGTGA