LOC108256642 cDNA ORF clone, Ictalurus punctatus(channel catfish)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC108256642
Entrez Gene ID	108256642
Full Name	hephaestin-like protein 1
Gene Type	protein-coding
Organism	Ictalurus punctatus(channel catfish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017453708.1	XP_017309197.1	hephaestin-like protein 1 isoform X1
XM_017453709.1	XP_017309198.1	hephaestin-like protein 1 isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC108256642 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC108256642 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OIc21974	XM_017453708.1 Latest version!	Ictalurus punctatus hephaestin-like protein 1 (LOC108256642), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OIc21975	XM_017453709.1 Latest version!	Ictalurus punctatus hephaestin-like protein 1 (LOC108256642), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC108256642 ( XM_017453708.1 ) cDNA ORF clone, Ictalurus punctatus(channel catfish) -> XP_017309197.1 Ictalurus punctatus hephaestin-like protein 1 (LOC108256642), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OIc21974
Clone ID Related Accession (Same CDS sequence)	XM_017453708.1
Accession Version	XM_017453708.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-05
Organism	Ictalurus punctatus(channel catfish)
Product	hephaestin-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030438.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictalurus punctatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCAA GCGTTCTGAG TTTCTCTCTG ACGTTTCTGA CCGTGCTCGG CTTGGGCCGG 
TGTCTTCAAA GGATATATTA TATCGGGATT CAGGAAGTGG ACTGGAATTA CGCACCAAGC 
GGAAAGAATC TAATCAATGG CCTGCAAATC GACCAAGACG AGCATGCCTC TATATTTCTG 
GACAGTGGTC CAGCACGGAT TGGAGGGGTG TATAAAAAAG CTGTGTATAA GCAGTACACA 
AATGAGACTT TCTCTAGTGA GATCCTCAAA CCAGAGTGGC TGGGCTTCCT TGGACCTGTG 
ATCCGTGCAG AAGTGGGTGA TGACATCGTA GTACACATGA AAAACTTTGC ATCTCGACCA 
TATTCAGTCC ATCCTCATGG AGTCTTCTAC AAGAAGGATT CTGAAGGTGC ATTATATCCG 
GATGGTACAT CTGGTCGAAA TAAAAAAGAT GATTCTGTGG GTCCTGGTGA AAGCTATACG 
TACACTTGGA GCGTGACAGA AGAGTTTGCC CCTACCAAAT CTGACCCCTC CTGCCTCACC 
TGGATCTACC ACTCCCATGT CGATGCACCT AAAGACATTG CATCAGGACT TATTGGAGTT 
CTGCTCACCT GCAATAAGGG AGTCCTAGAC AACTCGGGAA CTCGACGCTT TGATGTGGAT 
CAGGAGTTCT TCCTGATGTT CAGCGTGGTA GATGAAAACC TGAGCTGGTA CCTTAAAAAC 
AACATTGAGA GGTTCATACC TGATGCTGTT AATCTTAAAG ACAATGAAGA ATTTCATGAG 
AGCAACCTCA TGCACGCAAT CAACGGTTAT GTTTTTGGTA ACTTGCCACC GTTAAACGTG 
TGTGTCGGAC GCACTGTGTC TTGGCATCTC TTCGGCATTG GAAATGAAGT GGATATTCAT 
TCTGCCTACT TCCATGGCCA CACTCTGCTT GACCGGGGTC ACCGTACTGA TGTGCTCAGC 
CTATTTCCTG CCACTTTTGT CACGGCAAAA ATGGTTCCCA TGACAACAGG AAAATGGATG 
TTAAGTTGCC AGGTTAATGA CCACATGCAA GCAGGTATGC AGGGCTTCTA TGAAGTGTCT 
TTATGTGGGA AGGATGATGC GGCACCCACA GTGACTCCTG GCCATGAGAG ACATTTCTAT 
ATTGCTGCTG AAGAGCAGAT TTGGAATTAT GCCCCCTCTG GGATCAATCA ATTCACAAAT 
GAAAGGTTGG ATGAGAAAGA AAGTGATGCT GCAGAATTCT TCAGTAAAGA GGAAGGCAGG 
CTGGGCGGGA GCTACTGGAA AGTCAGGTAC ATTGGATACC ACGATGACAC GTTTACCACC 
AAGACTGAGC AACATGGCTC AGAACATCAT CTTGGCTTTC TGGGTCCAGT CATTAGAGGA 
GAATCTGGAG ATGTTATTGT GGTAACCTTT CGGAATATGG CATCAAAAAA CTACAGCATT 
CAACCTCATG GTCTGTCATA TGATAAACCT TACGAAGGGG CCACATATCA GGATGGTACT 
CAGAAAGCAG GGGCCAGTGT GGCCCCAGGT GAGACATTTA CATATCGATG GCAGGTGAGA 
GAAGGCCCCT CGGACAGTGA CCCACCCTGC CTGTCATATT TGTATTACTC AGCTAGTGAT 
CCTGTCCGCG ACACCAACTC TGGTTTGGTG GGACCGTTAC AGGTGTGCAA GAAAGGAGTT 
CTGGATGAGA GTGGTCGACA GCATATAGGG TCGGAGGTGC AGAAAGAGTT TTTCGTACTC 
TTCACTGTGA TCGACGAGAA TGAAAGCTGG TACCTGCAGA AGAATATTGA TGAGTTTGGT 
AGTGATCAGT CAGACGAGGA AAACCAAATA TTTCAGGAGA GCAACAAAAT GCACTGTGTA 
AACGGTTTCA TGTACGGGAA TCTTCCTGGC CTTGATTTGT GTGAGGGGGA CCATGTTGTG 
TGGCACATGC TGGGCTTGGG CACAGAGGTG GATCTCCACG GCGTTTACTT CCAGGGAAAC 
ACTTTTCAGC GGGACAGAAC TACGCAAGAC ACACTCAGCC TGTTTCCTCA CACCAGTGTC 
ACCGTCTCCA TGATACCAGA CAACAACGGT GTTTTTGGGC TGAGCTGTCG TACAACTGAT 
CACTACCGGG GTGGCATGCT CCAGCTGTAC ACCGTCAAAA GCTGCACACC GAGCCAGAAA 
CCTCAATCGG AAGGTACGAC CTTCCAACCT GATGTATATT TCTACTTAGC TGCTGAAGAA 
GTGGTTTGGA ACTATGCCCC AAACCGGACA TGGGAGTTGG AGAAACATCA CACGACTCTA 
CATGACAGCC CTGGAAATGT TTTCCTCAGT AAATCTGCGA ACCAGATTGG CCCTGAATAC 
AGAAAGGTGG TGTACCGTCA GTACACAGAT GACACCTTTG CAACCAAGAA GCCCAGAGGT 
CCTGATGAAG AGCATCTAGG CATTCTGGGT CCCATTCTCA GGATGGAGGT TGGTGAGAGG 
GTCCAGGTTG TGTTTAAGAA CAAAGCGAGT AGGCCATATT CAGTTCATGG TCATGGTGTG 
AAGACCATTG AGAAACACCT GGAACCTGTT CCTCCAGGTA ATGTTATAAG GTATAACTGG 
ACTATCAGTT CAGGTCCTGG ACGCACTGAG CCCAACTGCA TAACCTTTGC TTATTACTCG 
TCAGCTGACT ACGTCAAGGA TTTGGCGAGT GGTCTGGTTG GACCATTGGT AGTGTGTCGT 
AAAGGGACTC TGGACAAAGA CAGAAGCCGA AGCGATGTGG ATAAAGAATT CGCTCTGCTC 
TTCATGGTGT TTGATGAGAA CGAATCCTGG TATCTGGAGG ACAATATAAA AACATACCTG 
CACAAGGACC CAGAAACTTT TCATATACAC GACGAGAAAT TTATGGAGAG TAACATGATG 
CACGGTATTA ATGGAAAGCT GTATGCTAAC CTGCATGGAT TGAAGATGCA GCAAGGAGAT 
CAGACTGAGT GGTATTTAAT GGGGCTGGGA AACGAGATTG ACATGCACAC AGTACACTTT 
CACGCTCAGA GCTTCATATA CAAGACCGAT CACCCCCACA GAGCTGACGT GTATGACTTG 
TTCCCAGGAA CATTTAAGAC TGTGGAACTG GTAGCTGGAA CTCCTGGAAC GTGGCTCCTT 
CACTGCCATG TGACAGACCA CATCCATGCA GGCATGGAGA CGACCTTCAC CATCGACGCG 
GCAGATTCAA AAGTAGAGAA CCCTAAGGGA AATGGCTGCA CATGTTTGCA GAGTTTTCCA 
CGAATGCTAC TATTCATCAC AGTTGCTTTC TGGCTCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017309197.1
CDS	232..3513
Translation	MDASVLSFSLTFLTVLGLGRCLQRIYYIGIQEVDWNYAPSGKNLINGLQIDQDEHASIFLDSGPARIGGVYKKAVYKQYTNETFSSEILKPEWLGFLGPVIRAEVGDDIVVHMKNFASRPYSVHPHGVFYKKDSEGALYPDGTSGRNKKDDSVGPGESYTYTWSVTEEFAPTKSDPSCLTWIYHSHVDAPKDIASGLIGVLLTCNKGVLDNSGTRRFDVDQEFFLMFSVVDENLSWYLKNNIERFIPDAVNLKDNEEFHESNLMHAINGYVFGNLPPLNVCVGRTVSWHLFGIGNEVDIHSAYFHGHTLLDRGHRTDVLSLFPATFVTAKMVPMTTGKWMLSCQVNDHMQAGMQGFYEVSLCGKDDAAPTVTPGHERHFYIAAEEQIWNYAPSGINQFTNERLDEKESDAAEFFSKEEGRLGGSYWKVRYIGYHDDTFTTKTEQHGSEHHLGFLGPVIRGESGDVIVVTFRNMASKNYSIQPHGLSYDKPYEGATYQDGTQKAGASVAPGETFTYRWQVREGPSDSDPPCLSYLYYSASDPVRDTNSGLVGPLQVCKKGVLDESGRQHIGSEVQKEFFVLFTVIDENESWYLQKNIDEFGSDQSDEENQIFQESNKMHCVNGFMYGNLPGLDLCEGDHVVWHMLGLGTEVDLHGVYFQGNTFQRDRTTQDTLSLFPHTSVTVSMIPDNNGVFGLSCRTTDHYRGGMLQLYTVKSCTPSQKPQSEGTTFQPDVYFYLAAEEVVWNYAPNRTWELEKHHTTLHDSPGNVFLSKSANQIGPEYRKVVYRQYTDDTFATKKPRGPDEEHLGILGPILRMEVGERVQVVFKNKASRPYSVHGHGVKTIEKHLEPVPPGNVIRYNWTISSGPGRTEPNCITFAYYSSADYVKDLASGLVGPLVVCRKGTLDKDRSRSDVDKEFALLFMVFDENESWYLEDNIKTYLHKDPETFHIHDEKFMESNMMHGINGKLYANLHGLKMQQGDQTEWYLMGLGNEIDMHTVHFHAQSFIYKTDHPHRADVYDLFPGTFKTVELVAGTPGTWLLHCHVTDHIHAGMETTFTIDAADSKVENPKGNGCTCLQSFPRMLLFITVAFWLQ

Target ORF information:

RefSeq Version	XM_017453708.1
Organism	Ictalurus punctatus(channel catfish)
Definition	Ictalurus punctatus hephaestin-like protein 1 (LOC108256642), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017453708.1

ORF Insert Sequence:

ATGGATGCAA GCGTTCTGAG TTTCTCTCTG ACGTTTCTGA CCGTGCTCGG CTTGGGCCGG 
TGTCTTCAAA GGATATATTA TATCGGGATT CAGGAAGTGG ACTGGAATTA CGCACCAAGC 
GGAAAGAATC TAATCAATGG CCTGCAAATC GACCAAGACG AGCATGCCTC TATATTTCTG 
GACAGTGGTC CAGCACGGAT TGGAGGGGTG TATAAAAAAG CTGTGTATAA GCAGTACACA 
AATGAGACTT TCTCTAGTGA GATCCTCAAA CCAGAGTGGC TGGGCTTCCT TGGACCTGTG 
ATCCGTGCAG AAGTGGGTGA TGACATCGTA GTACACATGA AAAACTTTGC ATCTCGACCA 
TATTCAGTCC ATCCTCATGG AGTCTTCTAC AAGAAGGATT CTGAAGGTGC ATTATATCCG 
GATGGTACAT CTGGTCGAAA TAAAAAAGAT GATTCTGTGG GTCCTGGTGA AAGCTATACG 
TACACTTGGA GCGTGACAGA AGAGTTTGCC CCTACCAAAT CTGACCCCTC CTGCCTCACC 
TGGATCTACC ACTCCCATGT CGATGCACCT AAAGACATTG CATCAGGACT TATTGGAGTT 
CTGCTCACCT GCAATAAGGG AGTCCTAGAC AACTCGGGAA CTCGACGCTT TGATGTGGAT 
CAGGAGTTCT TCCTGATGTT CAGCGTGGTA GATGAAAACC TGAGCTGGTA CCTTAAAAAC 
AACATTGAGA GGTTCATACC TGATGCTGTT AATCTTAAAG ACAATGAAGA ATTTCATGAG 
AGCAACCTCA TGCACGCAAT CAACGGTTAT GTTTTTGGTA ACTTGCCACC GTTAAACGTG 
TGTGTCGGAC GCACTGTGTC TTGGCATCTC TTCGGCATTG GAAATGAAGT GGATATTCAT 
TCTGCCTACT TCCATGGCCA CACTCTGCTT GACCGGGGTC ACCGTACTGA TGTGCTCAGC 
CTATTTCCTG CCACTTTTGT CACGGCAAAA ATGGTTCCCA TGACAACAGG AAAATGGATG 
TTAAGTTGCC AGGTTAATGA CCACATGCAA GCAGGTATGC AGGGCTTCTA TGAAGTGTCT 
TTATGTGGGA AGGATGATGC GGCACCCACA GTGACTCCTG GCCATGAGAG ACATTTCTAT 
ATTGCTGCTG AAGAGCAGAT TTGGAATTAT GCCCCCTCTG GGATCAATCA ATTCACAAAT 
GAAAGGTTGG ATGAGAAAGA AAGTGATGCT GCAGAATTCT TCAGTAAAGA GGAAGGCAGG 
CTGGGCGGGA GCTACTGGAA AGTCAGGTAC ATTGGATACC ACGATGACAC GTTTACCACC 
AAGACTGAGC AACATGGCTC AGAACATCAT CTTGGCTTTC TGGGTCCAGT CATTAGAGGA 
GAATCTGGAG ATGTTATTGT GGTAACCTTT CGGAATATGG CATCAAAAAA CTACAGCATT 
CAACCTCATG GTCTGTCATA TGATAAACCT TACGAAGGGG CCACATATCA GGATGGTACT 
CAGAAAGCAG GGGCCAGTGT GGCCCCAGGT GAGACATTTA CATATCGATG GCAGGTGAGA 
GAAGGCCCCT CGGACAGTGA CCCACCCTGC CTGTCATATT TGTATTACTC AGCTAGTGAT 
CCTGTCCGCG ACACCAACTC TGGTTTGGTG GGACCGTTAC AGGTGTGCAA GAAAGGAGTT 
CTGGATGAGA GTGGTCGACA GCATATAGGG TCGGAGGTGC AGAAAGAGTT TTTCGTACTC 
TTCACTGTGA TCGACGAGAA TGAAAGCTGG TACCTGCAGA AGAATATTGA TGAGTTTGGT 
AGTGATCAGT CAGACGAGGA AAACCAAATA TTTCAGGAGA GCAACAAAAT GCACTGTGTA 
AACGGTTTCA TGTACGGGAA TCTTCCTGGC CTTGATTTGT GTGAGGGGGA CCATGTTGTG 
TGGCACATGC TGGGCTTGGG CACAGAGGTG GATCTCCACG GCGTTTACTT CCAGGGAAAC 
ACTTTTCAGC GGGACAGAAC TACGCAAGAC ACACTCAGCC TGTTTCCTCA CACCAGTGTC 
ACCGTCTCCA TGATACCAGA CAACAACGGT GTTTTTGGGC TGAGCTGTCG TACAACTGAT 
CACTACCGGG GTGGCATGCT CCAGCTGTAC ACCGTCAAAA GCTGCACACC GAGCCAGAAA 
CCTCAATCGG AAGGTACGAC CTTCCAACCT GATGTATATT TCTACTTAGC TGCTGAAGAA 
GTGGTTTGGA ACTATGCCCC AAACCGGACA TGGGAGTTGG AGAAACATCA CACGACTCTA 
CATGACAGCC CTGGAAATGT TTTCCTCAGT AAATCTGCGA ACCAGATTGG CCCTGAATAC 
AGAAAGGTGG TGTACCGTCA GTACACAGAT GACACCTTTG CAACCAAGAA GCCCAGAGGT 
CCTGATGAAG AGCATCTAGG CATTCTGGGT CCCATTCTCA GGATGGAGGT TGGTGAGAGG 
GTCCAGGTTG TGTTTAAGAA CAAAGCGAGT AGGCCATATT CAGTTCATGG TCATGGTGTG 
AAGACCATTG AGAAACACCT GGAACCTGTT CCTCCAGGTA ATGTTATAAG GTATAACTGG 
ACTATCAGTT CAGGTCCTGG ACGCACTGAG CCCAACTGCA TAACCTTTGC TTATTACTCG 
TCAGCTGACT ACGTCAAGGA TTTGGCGAGT GGTCTGGTTG GACCATTGGT AGTGTGTCGT 
AAAGGGACTC TGGACAAAGA CAGAAGCCGA AGCGATGTGG ATAAAGAATT CGCTCTGCTC 
TTCATGGTGT TTGATGAGAA CGAATCCTGG TATCTGGAGG ACAATATAAA AACATACCTG 
CACAAGGACC CAGAAACTTT TCATATACAC GACGAGAAAT TTATGGAGAG TAACATGATG 
CACGGTATTA ATGGAAAGCT GTATGCTAAC CTGCATGGAT TGAAGATGCA GCAAGGAGAT 
CAGACTGAGT GGTATTTAAT GGGGCTGGGA AACGAGATTG ACATGCACAC AGTACACTTT 
CACGCTCAGA GCTTCATATA CAAGACCGAT CACCCCCACA GAGCTGACGT GTATGACTTG 
TTCCCAGGAA CATTTAAGAC TGTGGAACTG GTAGCTGGAA CTCCTGGAAC GTGGCTCCTT 
CACTGCCATG TGACAGACCA CATCCATGCA GGCATGGAGA CGACCTTCAC CATCGACGCG 
GCAGATTCAA AAGTAGAGAA CCCTAAGGGA AATGGCTGCA CATGTTTGCA GAGTTTTCCA 
CGAATGCTAC TATTCATCAC AGTTGCTTTC TGGCTCCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108256642 ( XM_017453709.1 ) cDNA ORF clone, Ictalurus punctatus(channel catfish) -> XP_017309198.1 Ictalurus punctatus hephaestin-like protein 1 (LOC108256642), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OIc21975
Clone ID Related Accession (Same CDS sequence)	XM_017453709.1
Accession Version	XM_017453709.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-05
Organism	Ictalurus punctatus(channel catfish)
Product	hephaestin-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030438.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ictalurus punctatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCAA GCGTTCTGAG TTTCTCTCTG ACGTTTCTGA CCGTGCTCGG CTTGGGCCGG 
TGTCTTCAAA GGATATATTA TATCGGGATT CAGGAAGTGG ACTGGAATTA CGCACCAAGC 
GGAAAGAATC TAATCAATGG CCTGCAAATC GACCAAGACG AGCATGCCTC TATATTTCTG 
GACAGTGGTC CAGCACGGAT TGGAGGGGTG TATAAAAAAG CTGTGTATAA GCAGTACACA 
AATGAGACTT TCTCTAGTGA GATCCTCAAA CCAGAGTGGC TGGGCTTCCT TGGACCTGTG 
ATCCGTGCAG AAGTGGGTGA TGACATCGTA GTACACATGA AAAACTTTGC ATCTCGACCA 
TATTCAGTCC ATCCTCATGG AGTCTTCTAC AAGAAGGATT CTGAAGGTGC ATTATATCCG 
GATGGTACAT CTGGTCGAAA TAAAAAAGAT GATTCTGTGG GTCCTGGTGA AAGCTATACG 
TACACTTGGA GCGTGACAGA AGAGTTTGCC CCTACCAAAT CTGACCCCTC CTGCCTCACC 
TGGATCTACC ACTCCCATGT CGATGCACCT AAAGACATTG CATCAGGACT TATTGGAGTT 
CTGCTCACCT GCAATAAGGG AGTCCTAGAC AACTCGGGAA CTCGACGCTT TGATGTGGAT 
CAGGAGTTCT TCCTGATGTT CAGCGTGGTA GATGAAAACC TGAGCTGGTA CCTTAAAAAC 
AACATTGAGA GGTTCATACC TGATGCTGTT AATCTTAAAG ACAATGAAGA ATTTCATGAG 
AGCAACCTCA TGCACGCAAT CAACGGTTAT GTTTTTGGTA ACTTGCCACC GTTAAACGTG 
TGTGTCGGAC GCACTGTGTC TTGGCATCTC TTCGGCATTG GAAATGAAGT GGATATTCAT 
TCTGCCTACT TCCATGGCCA CACTCTGCTT GACCGGGGTC ACCGTACTGA TGTGCTCAGC 
CTATTTCCTG CCACTTTTGT CACGGCAAAA ATGGTTCCCA TGACAACAGG AAAATGGATG 
TTAAGTTGCC AGGTTAATGA CCACATGCAA GCAGGTATGC AGGGCTTCTA TGAAGTGTCT 
TTATGTGGGA AGGATGATGC GGCACCCACA GTGACTCCTG GCCATGAGAG ACATTTCTAT 
ATTGCTGCTG AAGAGCAGAT TTGGAATTAT GCCCCCTCTG GGATCAATCA ATTCACAAAT 
GAAAGGTTGG ATGAGAAAGA AAGTGATGCT GCAGAATTCT TCAGTAAAGA GGAAGGCAGG 
CTGGGCGGGA GCTACTGGAA AGTCAGGTAC ATTGGATACC ACGATGACAC GTTTACCACC 
AAGACTGAGC AACATGGCTC AGAACATCAT CTTGGCTTTC TGGGTCCAGT CATTAGAGGA 
GAATCTGGAG ATGTTATTGT GGTAACCTTT CGGAATATGG CATCAAAAAA CTACAGCATT 
CAACCTCATG GTCTGTCATA TGATAAACCT TACGAAGGGG CCACATATCA GGATGGTACT 
CAGAAAGCAG GGGCCAGTGT GGCCCCAGGT GAGACATTTA CATATCGATG GCAGGTGAGA 
GAAGGCCCCT CGGACAGTGA CCCACCCTGC CTGTCATATT TGTATTACTC AGCTAGTGAT 
CCTGTCCGCG ACACCAACTC TGGTTTGGTG GGACCGTTAC AGGTGTGCAA GAAAGGAGTT 
CTGGATGAGA GTGGTCGACA GCATATAGGG TCGGAGGTGC AGAAAGAGTT TTTCGTACTC 
TTCACTGTGA TCGACGAGAA TGAAAGCTGG TACCTGCAGA AGAATATTGA TGAGTTTGGT 
AGTGATCAGT CAGACGAGGA AAACCAAATA TTTCAGGAGA GCAACAAAAT GCACTGTGTA 
AACGGTTTCA TGTACGGGAA TCTTCCTGGC CTTGATTTGT GTGAGGGGGA CCATGTTGTG 
TGGCACATGC TGGGCTTGGG CACAGAGGTG GATCTCCACG GCGTTTACTT CCAGGGAAAC 
ACTTTTCAGC GGGACAGAAC TACGCAAGAC ACACTCAGCC TGTTTCCTCA CACCAGTGTC 
ACCGTCTCCA TGATACCAGA CAACAACGGT GTTTTTGGGC TGAGCTGTCG TACAACTGAT 
CACTACCGGG GTGGCATGCT CCAGCTGTAC ACCGTCAAAA GCTGCACACC GAGCCAGAAA 
CCTCAATCGG AAGGTACGAC CTTCCAACCT GATGTATATT TCTACTTAGC TGCTGAAGAA 
GTGGTTTGGA ACTATGCCCC AAACCGGACA TGGGAGTTGG AGAAACATCA CACGACTCTA 
CATGACAGCC CTGGAAATGT TTTCCTCAGT AAATCTGCGA ACCAGATTGG CCCTGAATAC 
AGAAAGGTGG TGTACCGTCA GTACACAGAT GACACCTTTG CAACCAAGAA GCCCAGAGGT 
CCTGATGAAG AGCATCTAGG CATTCTGGGT CCCATTCTCA GGATGGAGGT TGGTGAGAGG 
GTCCAGGTTG TGTTTAAGAA CAAAGCGAGT AGGCCATATT CAGTTCATGG TCATGGTGTG 
AAGACCATTG AGAAACACCT GGAACCTGTT CCTCCAGGTA ATGTTATAAG GTATAACTGG 
ACTATCAGTT CAGGTCCTGG ACGCACTGAG CCCAACTGCA TAACCTTTGC TTATTACTCG 
TCAGCTGACT ACGTCAAGGA TTTGGCGAGT GGTCTGGTTG GACCATTGGT AGTGTGTCGT 
AAAGGGACTC TGGACAAAGA CAGAAGCCGA AGCGATGTGG ATAAAGAATT CGCTCTGCTC 
TTCATGGTGT TTGATGAGAA CGAATCCTGG TATCTGGAGG ACAATATAAA AACATACCTG 
CACAAGGACC CAGAAACTTT TCATATACAC GACGAGAAAT TTATGGAGAG TAACATGATG 
CACGGTATTA ATGGAAAGCT GTATGCTAAC CTGCATGGAT TGAAGATGCA GCAAGGAGAT 
CAGACTGAGT GGTATTTAAT GGGGCTGGGA AACGAGATTG ACATGCACAC AGTACACTTT 
CACGCTCAGA GCTTCATATA CAAGACCGAT CACCCCCACA GAGCTGACGT GTATGACTTG 
TTCCCAGGAA CATTTAAGAC TGTGGAACTG GTAGCTGGAA CTCCTGGAAC GTGGCTCCTT 
CACTGCCATG TGACAGACCA CATCCATGCA GGCATGGAGA CGACCTTCAC CATCGACGGC 
TGCACATGTT TGCAGAGTTT TCCACGAATG CTACTATTCA TCACAGTTGC TTTCTGGCTC 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017309198.1
CDS	233..3478
Translation	MDASVLSFSLTFLTVLGLGRCLQRIYYIGIQEVDWNYAPSGKNLINGLQIDQDEHASIFLDSGPARIGGVYKKAVYKQYTNETFSSEILKPEWLGFLGPVIRAEVGDDIVVHMKNFASRPYSVHPHGVFYKKDSEGALYPDGTSGRNKKDDSVGPGESYTYTWSVTEEFAPTKSDPSCLTWIYHSHVDAPKDIASGLIGVLLTCNKGVLDNSGTRRFDVDQEFFLMFSVVDENLSWYLKNNIERFIPDAVNLKDNEEFHESNLMHAINGYVFGNLPPLNVCVGRTVSWHLFGIGNEVDIHSAYFHGHTLLDRGHRTDVLSLFPATFVTAKMVPMTTGKWMLSCQVNDHMQAGMQGFYEVSLCGKDDAAPTVTPGHERHFYIAAEEQIWNYAPSGINQFTNERLDEKESDAAEFFSKEEGRLGGSYWKVRYIGYHDDTFTTKTEQHGSEHHLGFLGPVIRGESGDVIVVTFRNMASKNYSIQPHGLSYDKPYEGATYQDGTQKAGASVAPGETFTYRWQVREGPSDSDPPCLSYLYYSASDPVRDTNSGLVGPLQVCKKGVLDESGRQHIGSEVQKEFFVLFTVIDENESWYLQKNIDEFGSDQSDEENQIFQESNKMHCVNGFMYGNLPGLDLCEGDHVVWHMLGLGTEVDLHGVYFQGNTFQRDRTTQDTLSLFPHTSVTVSMIPDNNGVFGLSCRTTDHYRGGMLQLYTVKSCTPSQKPQSEGTTFQPDVYFYLAAEEVVWNYAPNRTWELEKHHTTLHDSPGNVFLSKSANQIGPEYRKVVYRQYTDDTFATKKPRGPDEEHLGILGPILRMEVGERVQVVFKNKASRPYSVHGHGVKTIEKHLEPVPPGNVIRYNWTISSGPGRTEPNCITFAYYSSADYVKDLASGLVGPLVVCRKGTLDKDRSRSDVDKEFALLFMVFDENESWYLEDNIKTYLHKDPETFHIHDEKFMESNMMHGINGKLYANLHGLKMQQGDQTEWYLMGLGNEIDMHTVHFHAQSFIYKTDHPHRADVYDLFPGTFKTVELVAGTPGTWLLHCHVTDHIHAGMETTFTIDGCTCLQSFPRMLLFITVAFWLQ

Target ORF information:

RefSeq Version	XM_017453709.1
Organism	Ictalurus punctatus(channel catfish)
Definition	Ictalurus punctatus hephaestin-like protein 1 (LOC108256642), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017453709.1

ORF Insert Sequence:

ATGGATGCAA GCGTTCTGAG TTTCTCTCTG ACGTTTCTGA CCGTGCTCGG CTTGGGCCGG 
TGTCTTCAAA GGATATATTA TATCGGGATT CAGGAAGTGG ACTGGAATTA CGCACCAAGC 
GGAAAGAATC TAATCAATGG CCTGCAAATC GACCAAGACG AGCATGCCTC TATATTTCTG 
GACAGTGGTC CAGCACGGAT TGGAGGGGTG TATAAAAAAG CTGTGTATAA GCAGTACACA 
AATGAGACTT TCTCTAGTGA GATCCTCAAA CCAGAGTGGC TGGGCTTCCT TGGACCTGTG 
ATCCGTGCAG AAGTGGGTGA TGACATCGTA GTACACATGA AAAACTTTGC ATCTCGACCA 
TATTCAGTCC ATCCTCATGG AGTCTTCTAC AAGAAGGATT CTGAAGGTGC ATTATATCCG 
GATGGTACAT CTGGTCGAAA TAAAAAAGAT GATTCTGTGG GTCCTGGTGA AAGCTATACG 
TACACTTGGA GCGTGACAGA AGAGTTTGCC CCTACCAAAT CTGACCCCTC CTGCCTCACC 
TGGATCTACC ACTCCCATGT CGATGCACCT AAAGACATTG CATCAGGACT TATTGGAGTT 
CTGCTCACCT GCAATAAGGG AGTCCTAGAC AACTCGGGAA CTCGACGCTT TGATGTGGAT 
CAGGAGTTCT TCCTGATGTT CAGCGTGGTA GATGAAAACC TGAGCTGGTA CCTTAAAAAC 
AACATTGAGA GGTTCATACC TGATGCTGTT AATCTTAAAG ACAATGAAGA ATTTCATGAG 
AGCAACCTCA TGCACGCAAT CAACGGTTAT GTTTTTGGTA ACTTGCCACC GTTAAACGTG 
TGTGTCGGAC GCACTGTGTC TTGGCATCTC TTCGGCATTG GAAATGAAGT GGATATTCAT 
TCTGCCTACT TCCATGGCCA CACTCTGCTT GACCGGGGTC ACCGTACTGA TGTGCTCAGC 
CTATTTCCTG CCACTTTTGT CACGGCAAAA ATGGTTCCCA TGACAACAGG AAAATGGATG 
TTAAGTTGCC AGGTTAATGA CCACATGCAA GCAGGTATGC AGGGCTTCTA TGAAGTGTCT 
TTATGTGGGA AGGATGATGC GGCACCCACA GTGACTCCTG GCCATGAGAG ACATTTCTAT 
ATTGCTGCTG AAGAGCAGAT TTGGAATTAT GCCCCCTCTG GGATCAATCA ATTCACAAAT 
GAAAGGTTGG ATGAGAAAGA AAGTGATGCT GCAGAATTCT TCAGTAAAGA GGAAGGCAGG 
CTGGGCGGGA GCTACTGGAA AGTCAGGTAC ATTGGATACC ACGATGACAC GTTTACCACC 
AAGACTGAGC AACATGGCTC AGAACATCAT CTTGGCTTTC TGGGTCCAGT CATTAGAGGA 
GAATCTGGAG ATGTTATTGT GGTAACCTTT CGGAATATGG CATCAAAAAA CTACAGCATT 
CAACCTCATG GTCTGTCATA TGATAAACCT TACGAAGGGG CCACATATCA GGATGGTACT 
CAGAAAGCAG GGGCCAGTGT GGCCCCAGGT GAGACATTTA CATATCGATG GCAGGTGAGA 
GAAGGCCCCT CGGACAGTGA CCCACCCTGC CTGTCATATT TGTATTACTC AGCTAGTGAT 
CCTGTCCGCG ACACCAACTC TGGTTTGGTG GGACCGTTAC AGGTGTGCAA GAAAGGAGTT 
CTGGATGAGA GTGGTCGACA GCATATAGGG TCGGAGGTGC AGAAAGAGTT TTTCGTACTC 
TTCACTGTGA TCGACGAGAA TGAAAGCTGG TACCTGCAGA AGAATATTGA TGAGTTTGGT 
AGTGATCAGT CAGACGAGGA AAACCAAATA TTTCAGGAGA GCAACAAAAT GCACTGTGTA 
AACGGTTTCA TGTACGGGAA TCTTCCTGGC CTTGATTTGT GTGAGGGGGA CCATGTTGTG 
TGGCACATGC TGGGCTTGGG CACAGAGGTG GATCTCCACG GCGTTTACTT CCAGGGAAAC 
ACTTTTCAGC GGGACAGAAC TACGCAAGAC ACACTCAGCC TGTTTCCTCA CACCAGTGTC 
ACCGTCTCCA TGATACCAGA CAACAACGGT GTTTTTGGGC TGAGCTGTCG TACAACTGAT 
CACTACCGGG GTGGCATGCT CCAGCTGTAC ACCGTCAAAA GCTGCACACC GAGCCAGAAA 
CCTCAATCGG AAGGTACGAC CTTCCAACCT GATGTATATT TCTACTTAGC TGCTGAAGAA 
GTGGTTTGGA ACTATGCCCC AAACCGGACA TGGGAGTTGG AGAAACATCA CACGACTCTA 
CATGACAGCC CTGGAAATGT TTTCCTCAGT AAATCTGCGA ACCAGATTGG CCCTGAATAC 
AGAAAGGTGG TGTACCGTCA GTACACAGAT GACACCTTTG CAACCAAGAA GCCCAGAGGT 
CCTGATGAAG AGCATCTAGG CATTCTGGGT CCCATTCTCA GGATGGAGGT TGGTGAGAGG 
GTCCAGGTTG TGTTTAAGAA CAAAGCGAGT AGGCCATATT CAGTTCATGG TCATGGTGTG 
AAGACCATTG AGAAACACCT GGAACCTGTT CCTCCAGGTA ATGTTATAAG GTATAACTGG 
ACTATCAGTT CAGGTCCTGG ACGCACTGAG CCCAACTGCA TAACCTTTGC TTATTACTCG 
TCAGCTGACT ACGTCAAGGA TTTGGCGAGT GGTCTGGTTG GACCATTGGT AGTGTGTCGT 
AAAGGGACTC TGGACAAAGA CAGAAGCCGA AGCGATGTGG ATAAAGAATT CGCTCTGCTC 
TTCATGGTGT TTGATGAGAA CGAATCCTGG TATCTGGAGG ACAATATAAA AACATACCTG 
CACAAGGACC CAGAAACTTT TCATATACAC GACGAGAAAT TTATGGAGAG TAACATGATG 
CACGGTATTA ATGGAAAGCT GTATGCTAAC CTGCATGGAT TGAAGATGCA GCAAGGAGAT 
CAGACTGAGT GGTATTTAAT GGGGCTGGGA AACGAGATTG ACATGCACAC AGTACACTTT 
CACGCTCAGA GCTTCATATA CAAGACCGAT CACCCCCACA GAGCTGACGT GTATGACTTG 
TTCCCAGGAA CATTTAAGAC TGTGGAACTG GTAGCTGGAA CTCCTGGAAC GTGGCTCCTT 
CACTGCCATG TGACAGACCA CATCCATGCA GGCATGGAGA CGACCTTCAC CATCGACGGC 
TGCACATGTT TGCAGAGTTT TCCACGAATG CTACTATTCA TCACAGTTGC TTTCTGGCTC 
CAGTGA