LOC100304648 cDNA ORF clone, Ictalurus punctatus(channel catfish)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100304648
Entrez Gene ID	100304648
Full Name	NK-lysin type 1
Gene Type	protein-coding
Organism	Ictalurus punctatus(channel catfish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001200208.1	NP_001187137.1	NK-lysin type 1 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.
Liu S, Zhang Y, Zhou Z, Waldbieser G, Sun F, Lu J, Zhang J, Jiang Y, Zhang H, Wang X, Rajendran KV, Khoo L, Kucuktas H, Peatman E, Liu Z
BMC genomics13595(2012 Nov)

NK-lysin of channel catfish: gene triplication, sequence variation, and expression analysis.
Wang Q, Wang Y, Xu P, Liu Z
Molecular immunology43(10)1676-86(2006 Apr)

Characterization of a NK-lysin antimicrobial peptide gene from channel catfish.
Wang Q, Bao B, Wang Y, Peatman E, Liu Z
Fish & shellfish immunology20(3)419-26(2006 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC100304648 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100304648 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OIc39101	NM_001200208.1 Latest version!	Ictalurus punctatus NK-lysin type 1 (LOC100304648), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100304648 ( NM_001200208.1 ) cDNA ORF clone, Ictalurus punctatus(channel catfish) -> NP_001187137.1 Ictalurus punctatus NK-lysin type 1 (LOC100304648), mRNA.

Price & Availability↑

CloneID	OIc39101
Clone ID Related Accession (Same CDS sequence)	NM_001200208.1
Accession Version	NM_001200208.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Ictalurus punctatus(channel catfish)
Product	NK-lysin type 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY934592.1. ##Evidence-Data-START## Transcript exon combination :: AY934592.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00739092, SAMN00740394 [ECO:0000348] ##Evidence-Data-END##

ATGTTCTGGA ACCTCCTCGT TGCTTCTTTC TTCATAGGCT CAGCCTGTGC AATGCACATG 
GAATACCTGA GAGTTGACTC TGCTGAGGAA CTCCTTGATG GGTCTTTGGA TTCTACTGAT 
GAGGATGAGG ACTTGGCGAT GTCTGAGACA CAGCTACTCC CTGGTGCCTG TTGGGCGTGT 
CAGTGGGCCA TGAAGAAAGT GAAAAAACAA CTTGGCAATA ATCCAACTGT GGACATTATT 
AAAGCACAAT TGAAGAAAGT CTGCAATTCA ATCGGATTCC TTCGAGGTCT CTGCAAGAAA 
ATGATTAACA AGTACTTGGA TACCTTGGTG GAGGAACTTT CTACCACAGA TGATCCAACA 
ACAATCTGTG GTAATCTTGG TATTTGCAAG TCATTGAGCA TGCTGGAATT GTTCCAAGCT 
TTTCCACAAC ACCACAAGCA GATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001187137.1
CDS	47..493
Translation	MFWNLLVASFFIGSACAMHMEYLRVDSAEELLDGSLDSTDEDEDLAMSETQLLPGACWACQWAMKKVKKQLGNNPTVDIIKAQLKKVCNSIGFLRGLCKKMINKYLDTLVEELSTTDDPTTICGNLGICKSLSMLELFQAFPQHHKQI

Target ORF information:

RefSeq Version	NM_001200208.1
Organism	Ictalurus punctatus(channel catfish)
Definition	Ictalurus punctatus NK-lysin type 1 (LOC100304648), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001200208.1

ORF Insert Sequence:

ATGTTCTGGA ACCTCCTCGT TGCTTCTTTC TTCATAGGCT CAGCCTGTGC AATGCACATG 
GAATACCTGA GAGTTGACTC TGCTGAGGAA CTCCTTGATG GGTCTTTGGA TTCTACTGAT 
GAGGATGAGG ACTTGGCGAT GTCTGAGACA CAGCTACTCC CTGGTGCCTG TTGGGCGTGT 
CAGTGGGCCA TGAAGAAAGT GAAAAAACAA CTTGGCAATA ATCCAACTGT GGACATTATT 
AAAGCACAAT TGAAGAAAGT CTGCAATTCA ATCGGATTCC TTCGAGGTCT CTGCAAGAAA 
ATGATTAACA AGTACTTGGA TACCTTGGTG GAGGAACTTT CTACCACAGA TGATCCAACA 
ACAATCTGTG GTAATCTTGG TATTTGCAAG TCATTGAGCA TGCTGGAATT GTTCCAAGCT 
TTTCCACAAC ACCACAAGCA GATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.
BMC genomics13595(2012 Nov)
Liu S,Zhang Y,Zhou Z,Waldbieser G,Sun F,Lu J,Zhang J,Jiang Y,Zhang H,Wang X,Rajendran KV,Khoo L,Kucuktas H,Peatman E,Liu Z

NK-lysin of channel catfish: gene triplication, sequence variation, and expression analysis.
Molecular immunology43(10)1676-86(2006 Apr)
Wang Q,Wang Y,Xu P,Liu Z

Characterization of a NK-lysin antimicrobial peptide gene from channel catfish.
Fish & shellfish immunology20(3)419-26(2006 Mar)
Wang Q,Bao B,Wang Y,Peatman E,Liu Z