LOC105847151 cDNA ORF clone, Hydra vulgaris

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105847151
Entrez Gene ID	105847151
Full Name	muscarinic acetylcholine receptor M2-like
Gene Type	protein-coding
Organism	Hydra vulgaris

mRNA and Protein(s)

mRNA	Protein	Name
XM_012706544.1	XP_012561998.1	muscarinic acetylcholine receptor M2-like

Pathways from BioSystems
Gene Ontology

The following LOC105847151 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105847151 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHc23663	XM_012706544.1 Latest version!	Hydra vulgaris muscarinic acetylcholine receptor M2-like (LOC105847151), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105847151 ( XM_012706544.1 ) cDNA ORF clone, Hydra vulgaris -> XP_012561998.1 Hydra vulgaris muscarinic acetylcholine receptor M2-like (LOC105847151), mRNA.

Price & Availability↑

CloneID	OHc23663
Clone ID Related Accession (Same CDS sequence)	XM_012706544.1
Accession Version	XM_012706544.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-07
Organism	Hydra vulgaris
Product	muscarinic acetylcholine receptor M2-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004166874.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Hydra vulgaris Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGAGTTTCC TAAATAACAC TCATTTTTCT GAATGCTGTG GTCACACTTC GAATCATCAT 
AGACCTATTG TACAAACTGT TCTTATTTTT TTAGGGGTTG GAATTTTAAG CTTGACAACT 
GTATTTGGAA ACTTACTTAT TATTTTAGCA TACTACGATA ACAAAAAAAT TCGAAATCTT 
GCAAACATCC CCATAATTTC ATTGGCTTTT ACAGACTTGT TTATAGGCTT ATATCCTATG 
AATGTTAATG TATTAGAAAT GACATTAAGG TATTGGCCTT TAGGCCATGT AATGTGTAAT 
GTATCATTGA CTTTTGATTA CGTATGCGTA CAAACCTCAA TTAATCATAT AGTCTTAATT 
AATTTTGATC GTTATCTATC AATTAAGTCT CCAATGAAAC ACCGGCTACA GCAAAAAACT 
CCAAAAGTTA TGTTTAGACT GTTTGTTGTA TGGTTTTTTG GTATTTTGAT TTGGGTTCCA 
TATATCAATA GTTACATGTA TGTATTCCGA GATAAGTTTC ATGATAAGAA ATGCTACACA 
GACTTTTTAG CTAACAACAC TTCCAGTTAT AAAACAATCA TGTTTATTGT TACATCTGTT 
GTGGGGTATT TTATACCGTT AGGTATAGTT TTGACAATGT ATTTTAAGAT GTATAACATA 
ATAAAGAAGC AACTCTATGA ATCTAACCAA ACTCAATGTG CTCTTCGACC TCAAGAAACA 
AAAGAACATA TTGAAATAGG CAATCTTGCG TACAACCCCG TAAGTTGTTC TAATGCTACG 
GAAACTAGCT GCTACTTTGT GCCTTCGACA AATTCTTTAA AACAAACGTA TGAGTATCAA 
AAAAAACAAC TCGATTCAAT AGTTAAGCAA AAGAAAATAC TGAGACACAA GAAGTCATTG 
CGCATGATCG CAAGCATAAT ACTAGCATTT CTTATTACTG GGTTACCTCT TAATGCTCAA 
TGGTTTGTAA ATGGGATTTG CAAAAAGTGT TATAATGGAG TATTGTTTGA CATTTTTAAC 
TTCATAACTT ATCCAAACTC AACAGTAAAT CCGTTTTTAT ACGCATTTGT AAGCCGGGCT 
TTTCGTTTAC AATATAAAAG AATTTTATTT GGAAAAAAAA GGTTTCGAGA CGCTGAACGT 
CAAATCATAA ATAATTCAAT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012561998.1
CDS	1..1164
Translation	MSFLNNTHFSECCGHTSNHHRPIVQTVLIFLGVGILSLTTVFGNLLIILAYYDNKKIRNLANIPIISLAFTDLFIGLYPMNVNVLEMTLRYWPLGHVMCNVSLTFDYVCVQTSINHIVLINFDRYLSIKSPMKHRLQQKTPKVMFRLFVVWFFGILIWVPYINSYMYVFRDKFHDKKCYTDFLANNTSSYKTIMFIVTSVVGYFIPLGIVLTMYFKMYNIIKKQLYESNQTQCALRPQETKEHIEIGNLAYNPVSCSNATETSCYFVPSTNSLKQTYEYQKKQLDSIVKQKKILRHKKSLRMIASIILAFLITGLPLNAQWFVNGICKKCYNGVLFDIFNFITYPNSTVNPFLYAFVSRAFRLQYKRILFGKKRFRDAERQIINNSI

Target ORF information:

RefSeq Version	XM_012706544.1
Organism	Hydra vulgaris
Definition	Hydra vulgaris muscarinic acetylcholine receptor M2-like (LOC105847151), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012706544.1

ORF Insert Sequence:

ATGAGTTTCC TAAATAACAC TCATTTTTCT GAATGCTGTG GTCACACTTC GAATCATCAT 
AGACCTATTG TACAAACTGT TCTTATTTTT TTAGGGGTTG GAATTTTAAG CTTGACAACT 
GTATTTGGAA ACTTACTTAT TATTTTAGCA TACTACGATA ACAAAAAAAT TCGAAATCTT 
GCAAACATCC CCATAATTTC ATTGGCTTTT ACAGACTTGT TTATAGGCTT ATATCCTATG 
AATGTTAATG TATTAGAAAT GACATTAAGG TATTGGCCTT TAGGCCATGT AATGTGTAAT 
GTATCATTGA CTTTTGATTA CGTATGCGTA CAAACCTCAA TTAATCATAT AGTCTTAATT 
AATTTTGATC GTTATCTATC AATTAAGTCT CCAATGAAAC ACCGGCTACA GCAAAAAACT 
CCAAAAGTTA TGTTTAGACT GTTTGTTGTA TGGTTTTTTG GTATTTTGAT TTGGGTTCCA 
TATATCAATA GTTACATGTA TGTATTCCGA GATAAGTTTC ATGATAAGAA ATGCTACACA 
GACTTTTTAG CTAACAACAC TTCCAGTTAT AAAACAATCA TGTTTATTGT TACATCTGTT 
GTGGGGTATT TTATACCGTT AGGTATAGTT TTGACAATGT ATTTTAAGAT GTATAACATA 
ATAAAGAAGC AACTCTATGA ATCTAACCAA ACTCAATGTG CTCTTCGACC TCAAGAAACA 
AAAGAACATA TTGAAATAGG CAATCTTGCG TACAACCCCG TAAGTTGTTC TAATGCTACG 
GAAACTAGCT GCTACTTTGT GCCTTCGACA AATTCTTTAA AACAAACGTA TGAGTATCAA 
AAAAAACAAC TCGATTCAAT AGTTAAGCAA AAGAAAATAC TGAGACACAA GAAGTCATTG 
CGCATGATCG CAAGCATAAT ACTAGCATTT CTTATTACTG GGTTACCTCT TAATGCTCAA 
TGGTTTGTAA ATGGGATTTG CAAAAAGTGT TATAATGGAG TATTGTTTGA CATTTTTAAC 
TTCATAACTT ATCCAAACTC AACAGTAAAT CCGTTTTTAT ACGCATTTGT AAGCCGGGCT 
TTTCGTTTAC AATATAAAAG AATTTTATTT GGAAAAAAAA GGTTTCGAGA CGCTGAACGT 
CAAATCATAA ATAATTCAAT CTAA