LOC100212843 cDNA ORF clone, Hydra vulgaris

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100212843
Entrez Gene ID	100212843
Full Name	probable ATP-dependent RNA helicase DDX56
Gene Type	protein-coding
Organism	Hydra vulgaris

mRNA and Protein(s)

mRNA	Protein	Name
XM_002156395.3	XP_002156431.2	probable ATP-dependent RNA helicase DDX56

Pathways from BioSystems
Gene Ontology

The following LOC100212843 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100212843 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHc12417	XM_002156395.3 Latest version!	Hydra vulgaris probable ATP-dependent RNA helicase DDX56 (LOC100212843), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100212843 ( XM_002156395.3 ) cDNA ORF clone, Hydra vulgaris -> XP_002156431.2 Hydra vulgaris probable ATP-dependent RNA helicase DDX56 (LOC100212843), mRNA.

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CloneID	OHc12417
Clone ID Related Accession (Same CDS sequence)	XM_002156395.3
Accession Version	XM_002156395.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-07
Organism	Hydra vulgaris
Product	probable ATP-dependent RNA helicase DDX56
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004175495.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 8, 2015 this sequence version replaced XM_002156395.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Hydra vulgaris Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTCTA ATTTTACTGA TTTTGATATA GATGATAGAC TCGTTAAAGC TATTTCCAAG 
CTTGGATGGG CTACTCCAAG TGAAATACAG AAGCGAGCAA TTCCTCCTGC TTTAGAAGGA 
AAGGATATAA TTATACGTGC CAAAACAGGT TCAGGAAAAA CAGCAGCGTA TCTAATTCCG 
TTAATTCAAA AAATATTGAA GAATAAAGAA TCAAACAAAC CTAAAACTTT AAGTGTTGTT 
TTAGTTCCCT CTAAAGAGCT TTGTAAACAA TCTTATCGAA ATGCGCTCGA TCTCACCTCG 
TATTGTTCTA AACTTGTTTC AGTTGTAGAC TTAGGAAATT CCACAGTTCA GAGTTCTTCT 
TCTTTAATTA ATAATGCTGA TATTTTAATT AGTACACCAA GTAAAATCCT TGCACATATA 
AATAATAAAA CAATTAATCT AAAAGATTTT TTAGATTATC TTATACTTGA TGAAGCTGAT 
ATGATGTTTT CATATGGTTA TGAGCAAGAT TTAAAAACAA TTACAACTTC ATTGCCTAAA 
ATTTACCAAG CATTGTTAGT GTCTGCAACA ATAAGTGAAG ATATAAAATG TTTAGAAGCA 
TTAATTCTTA ACAAACCAGT TATTTTAAAG TTAGAGGAGT CACATTTACC AGAAAAGGAG 
AAGCTTAACC AGTTTGTTAT TAAATGTGAG TCAAGTGATA AGTATCTTCT TATTTATGCT 
TTATTCAAAT TAAACTTGGT GCAAGGTAAA ACATTAATAT TTGTCAATTC TATTGATAGA 
TGTTATCGAT TAAAGTTGTT TCTTGAACAG TTTTACATTC GTACATGTGT TTTAAACTCT 
GAATTACCTC AAAGCTCAAG AATCCACATA GTTGATGAAT TTAATCGTGG TGTTTATGAT 
ATTGTTATAG CAACAGATGA AGCTGTTGTC ATTAATACAA ATTTATCAAA CACAAATCAG 
AATAAAGAGA AAACAAAAAA AAATAAAAAA GCAATGAAAA CTAAAAAAGA TTATGCTGTT 
GCTCGAGGAA TTGATTTTCA AGATGTTGAC AATGTTCTTA ATTTTGATTT TCCAGAAACT 
GGAGATGCAT ATATACACCG AGTTGGAAGA ACAGCACGTG GAAATAATCA TGGCACAGCC 
CTATCCTTTG TTGCAAGTAG TGAAGATCAA AAGCATTTAA TGATGGTTGA GGAAAAACTT 
AAAACAGATG TCATTGCTAA AGAAAATGCA TTAAAACCGT ATAATTTTAA AATTGAAGAA 
ATTGAAGGTC TGCGCTATCG TGTCAATGAT GTCATTAATA GTGTTACTCG GATCAAAGTT 
AAAGACGCAA GATTTAAAGA AATTAAAAGT GAGATTTTGC ATTCAAATAA ATTAAAAATG 
TATTTTGAAG AGAATCCAAA AGATCTCAGA GTACTTCGAC ATGATAAAAT ACTGAAACCA 
ACTGATCAAC AGCCTCATAT GAAAGACGTA CCTGAATATC TTGTACCTAG TTCTTTAAGA 
CATATCATGG TCAAATCTAA AAGAAAACGT AAGCAATCAA ATATTCAACA TGAAAAGATA 
AAAAAGAAAA AGACAGACCC GTTAAAAACT TTTAAATATA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002156431.2
CDS	36..1640
Translation	MISNFTDFDIDDRLVKAISKLGWATPSEIQKRAIPPALEGKDIIIRAKTGSGKTAAYLIPLIQKILKNKESNKPKTLSVVLVPSKELCKQSYRNALDLTSYCSKLVSVVDLGNSTVQSSSSLINNADILISTPSKILAHINNKTINLKDFLDYLILDEADMMFSYGYEQDLKTITTSLPKIYQALLVSATISEDIKCLEALILNKPVILKLEESHLPEKEKLNQFVIKCESSDKYLLIYALFKLNLVQGKTLIFVNSIDRCYRLKLFLEQFYIRTCVLNSELPQSSRIHIVDEFNRGVYDIVIATDEAVVINTNLSNTNQNKEKTKKNKKAMKTKKDYAVARGIDFQDVDNVLNFDFPETGDAYIHRVGRTARGNNHGTALSFVASSEDQKHLMMVEEKLKTDVIAKENALKPYNFKIEEIEGLRYRVNDVINSVTRIKVKDARFKEIKSEILHSNKLKMYFEENPKDLRVLRHDKILKPTDQQPHMKDVPEYLVPSSLRHIMVKSKRKRKQSNIQHEKIKKKKTDPLKTFKYK

Target ORF information:

RefSeq Version	XM_002156395.3
Organism	Hydra vulgaris
Definition	Hydra vulgaris probable ATP-dependent RNA helicase DDX56 (LOC100212843), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002156395.3

ORF Insert Sequence:

ATGATTTCTA ATTTTACTGA TTTTGATATA GATGATAGAC TCGTTAAAGC TATTTCCAAG 
CTTGGATGGG CTACTCCAAG TGAAATACAG AAGCGAGCAA TTCCTCCTGC TTTAGAAGGA 
AAGGATATAA TTATACGTGC CAAAACAGGT TCAGGAAAAA CAGCAGCGTA TCTAATTCCG 
TTAATTCAAA AAATATTGAA GAATAAAGAA TCAAACAAAC CTAAAACTTT AAGTGTTGTT 
TTAGTTCCCT CTAAAGAGCT TTGTAAACAA TCTTATCGAA ATGCGCTCGA TCTCACCTCG 
TATTGTTCTA AACTTGTTTC AGTTGTAGAC TTAGGAAATT CCACAGTTCA GAGTTCTTCT 
TCTTTAATTA ATAATGCTGA TATTTTAATT AGTACACCAA GTAAAATCCT TGCACATATA 
AATAATAAAA CAATTAATCT AAAAGATTTT TTAGATTATC TTATACTTGA TGAAGCTGAT 
ATGATGTTTT CATATGGTTA TGAGCAAGAT TTAAAAACAA TTACAACTTC ATTGCCTAAA 
ATTTACCAAG CATTGTTAGT GTCTGCAACA ATAAGTGAAG ATATAAAATG TTTAGAAGCA 
TTAATTCTTA ACAAACCAGT TATTTTAAAG TTAGAGGAGT CACATTTACC AGAAAAGGAG 
AAGCTTAACC AGTTTGTTAT TAAATGTGAG TCAAGTGATA AGTATCTTCT TATTTATGCT 
TTATTCAAAT TAAACTTGGT GCAAGGTAAA ACATTAATAT TTGTCAATTC TATTGATAGA 
TGTTATCGAT TAAAGTTGTT TCTTGAACAG TTTTACATTC GTACATGTGT TTTAAACTCT 
GAATTACCTC AAAGCTCAAG AATCCACATA GTTGATGAAT TTAATCGTGG TGTTTATGAT 
ATTGTTATAG CAACAGATGA AGCTGTTGTC ATTAATACAA ATTTATCAAA CACAAATCAG 
AATAAAGAGA AAACAAAAAA AAATAAAAAA GCAATGAAAA CTAAAAAAGA TTATGCTGTT 
GCTCGAGGAA TTGATTTTCA AGATGTTGAC AATGTTCTTA ATTTTGATTT TCCAGAAACT 
GGAGATGCAT ATATACACCG AGTTGGAAGA ACAGCACGTG GAAATAATCA TGGCACAGCC 
CTATCCTTTG TTGCAAGTAG TGAAGATCAA AAGCATTTAA TGATGGTTGA GGAAAAACTT 
AAAACAGATG TCATTGCTAA AGAAAATGCA TTAAAACCGT ATAATTTTAA AATTGAAGAA 
ATTGAAGGTC TGCGCTATCG TGTCAATGAT GTCATTAATA GTGTTACTCG GATCAAAGTT 
AAAGACGCAA GATTTAAAGA AATTAAAAGT GAGATTTTGC ATTCAAATAA ATTAAAAATG 
TATTTTGAAG AGAATCCAAA AGATCTCAGA GTACTTCGAC ATGATAAAAT ACTGAAACCA 
ACTGATCAAC AGCCTCATAT GAAAGACGTA CCTGAATATC TTGTACCTAG TTCTTTAAGA 
CATATCATGG TCAAATCTAA AAGAAAACGT AAGCAATCAA ATATTCAACA TGAAAAGATA 
AAAAAGAAAA AGACAGACCC GTTAAAAACT TTTAAATATA AGTAA