CD40LG cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CD40LG
Entrez Gene ID	959
Full Name	CD40 ligand
Synonyms	CD154,CD40L,HIGM1,IGM,IMD3,T-BAM,TNFSF5,TRAP,gp39,hCD40L
General protein information	Preferred Names CD40 ligand Names CD40 ligand CD40 antigen ligand CD40-L T-B cell-activating molecule T-cell antigen Gp39 TNF-related activation protein tumor necrosis factor (ligand) superfamily member 5
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xq26.3
Summary	The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008].
Disorder MIM:	300386
Disorder Html:	Immunodeficiency, X-linked, with hyper-IgM, 308230 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000074.2	NP_000065.1	CD40 ligand
NM_000074.3	NP_000065.1	CD40 ligand

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	CD40LG	NP_990064.1
Xenopus tropicalis (tropical clawed frog)	cd40lg	XP_002932670.1
Canis lupus familiaris (dog)	CD40LG	NP_001002981.1
Homo sapiens (human)	CD40LG	NP_000065.1
	CD40LG	NP_001028011.1
Bos taurus (cattle)	CD40LG	NP_777049.1
Rattus norvegicus (Norway rat)	Cd40lg	NP_445805.1
Pan troglodytes (chimpanzee)	CD40LG	XP_529175.2
Mus musculus (house mouse)	Cd40lg	NP_035746.2

Gene Ontology

The following CD40LG gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CD40LG cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18096	NM_000074.3 Latest version!	Homo sapiens CD40 ligand (CD40LG), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu18096	NM_000074.2	Homo sapiens CD40 ligand (CD40LG), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CD40LG ( NM_000074.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000065.1 Homo sapiens CD40 ligand (CD40LG), mRNA.

Price & Availability↑

CloneID	OHu18096
Clone ID Related Accession (Same CDS sequence)	NM_000074.2 , NM_000074.3
Accession Version	NM_000074.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-11
Organism	Homo sapiens(human)
Product	CD40 ligand
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC071754.1 and X67878.1. This sequence is a reference standard in the RefSeqGene project. On Jan 29, 2005 this sequence version replaced NM_000074.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC071754.1, X68550.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##

ATGATCGAAA CATACAACCA AACTTCTCCC CGATCTGCGG CCACTGGACT GCCCATCAGC 
ATGAAAATTT TTATGTATTT ACTTACTGTT TTTCTTATCA CCCAGATGAT TGGGTCAGCA 
CTTTTTGCTG TGTATCTTCA TAGAAGGTTG GACAAGATAG AAGATGAAAG GAATCTTCAT 
GAAGATTTTG TATTCATGAA AACGATACAG AGATGCAACA CAGGAGAAAG ATCCTTATCC 
TTACTGAACT GTGAGGAGAT TAAAAGCCAG TTTGAAGGCT TTGTGAAGGA TATAATGTTA 
AACAAAGAGG AGACGAAGAA AGAAAACAGC TTTGAAATGC AAAAAGGTGA TCAGAATCCT 
CAAATTGCGG CACATGTCAT AAGTGAGGCC AGCAGTAAAA CAACATCTGT GTTACAGTGG 
GCTGAAAAAG GATACTACAC CATGAGCAAC AACTTGGTAA CCCTGGAAAA TGGGAAACAG 
CTGACCGTTA AAAGACAAGG ACTCTATTAT ATCTATGCCC AAGTCACCTT CTGTTCCAAT 
CGGGAAGCTT CGAGTCAAGC TCCATTTATA GCCAGCCTCT GCCTAAAGTC CCCCGGTAGA 
TTCGAGAGAA TCTTACTCAG AGCTGCAAAT ACCCACAGTT CCGCCAAACC TTGCGGGCAA 
CAATCCATTC ACTTGGGAGG AGTATTTGAA TTGCAACCAG GTGCTTCGGT GTTTGTCAAT 
GTGACTGATC CAAGCCAAGT GAGCCATGGC ACTGGCTTCA CGTCCTTTGG CTTACTCAAA 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000065.1
CDS	73..858
Translation	MIETYNQTSPRSAATGLPISMKIFMYLLTVFLITQMIGSALFAVYLHRRLDKIEDERNLHEDFVFMKTIQRCNTGERSLSLLNCEEIKSQFEGFVKDIMLNKEETKKENSFEMQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL

Target ORF information:

RefSeq Version	NM_000074.2
Organism	Homo sapiens(human)
Definition	Homo sapiens CD40 ligand (CD40LG), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000074.2

ORF Insert Sequence:

ATGATCGAAA CATACAACCA AACTTCTCCC CGATCTGCGG CCACTGGACT GCCCATCAGC 
ATGAAAATTT TTATGTATTT ACTTACTGTT TTTCTTATCA CCCAGATGAT TGGGTCAGCA 
CTTTTTGCTG TGTATCTTCA TAGAAGGTTG GACAAGATAG AAGATGAAAG GAATCTTCAT 
GAAGATTTTG TATTCATGAA AACGATACAG AGATGCAACA CAGGAGAAAG ATCCTTATCC 
TTACTGAACT GTGAGGAGAT TAAAAGCCAG TTTGAAGGCT TTGTGAAGGA TATAATGTTA 
AACAAAGAGG AGACGAAGAA AGAAAACAGC TTTGAAATGC AAAAAGGTGA TCAGAATCCT 
CAAATTGCGG CACATGTCAT AAGTGAGGCC AGCAGTAAAA CAACATCTGT GTTACAGTGG 
GCTGAAAAAG GATACTACAC CATGAGCAAC AACTTGGTAA CCCTGGAAAA TGGGAAACAG 
CTGACCGTTA AAAGACAAGG ACTCTATTAT ATCTATGCCC AAGTCACCTT CTGTTCCAAT 
CGGGAAGCTT CGAGTCAAGC TCCATTTATA GCCAGCCTCT GCCTAAAGTC CCCCGGTAGA 
TTCGAGAGAA TCTTACTCAG AGCTGCAAAT ACCCACAGTT CCGCCAAACC TTGCGGGCAA 
CAATCCATTC ACTTGGGAGG AGTATTTGAA TTGCAACCAG GTGCTTCGGT GTTTGTCAAT 
GTGACTGATC CAAGCCAAGT GAGCCATGGC ACTGGCTTCA CGTCCTTTGG CTTACTCAAA 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CD40LG ( NM_000074.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000065.1 Homo sapiens CD40 ligand (CD40LG), mRNA.

Price & Availability↑

CloneID	OHu18096
Clone ID Related Accession (Same CDS sequence)	NM_000074.2 , NM_000074.3
Accession Version	NM_000074.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	CD40 ligand
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL135783.6, BC071754.1 and X67878.1. On Aug 31, 2019 this sequence version replaced NM_000074.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC071754.1, L07414.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##

ATGATCGAAA CATACAACCA AACTTCTCCC CGATCTGCGG CCACTGGACT GCCCATCAGC 
ATGAAAATTT TTATGTATTT ACTTACTGTT TTTCTTATCA CCCAGATGAT TGGGTCAGCA 
CTTTTTGCTG TGTATCTTCA TAGAAGGTTG GACAAGATAG AAGATGAAAG GAATCTTCAT 
GAAGATTTTG TATTCATGAA AACGATACAG AGATGCAACA CAGGAGAAAG ATCCTTATCC 
TTACTGAACT GTGAGGAGAT TAAAAGCCAG TTTGAAGGCT TTGTGAAGGA TATAATGTTA 
AACAAAGAGG AGACGAAGAA AGAAAACAGC TTTGAAATGC AAAAAGGTGA TCAGAATCCT 
CAAATTGCGG CACATGTCAT AAGTGAGGCC AGCAGTAAAA CAACATCTGT GTTACAGTGG 
GCTGAAAAAG GATACTACAC CATGAGCAAC AACTTGGTAA CCCTGGAAAA TGGGAAACAG 
CTGACCGTTA AAAGACAAGG ACTCTATTAT ATCTATGCCC AAGTCACCTT CTGTTCCAAT 
CGGGAAGCTT CGAGTCAAGC TCCATTTATA GCCAGCCTCT GCCTAAAGTC CCCCGGTAGA 
TTCGAGAGAA TCTTACTCAG AGCTGCAAAT ACCCACAGTT CCGCCAAACC TTGCGGGCAA 
CAATCCATTC ACTTGGGAGG AGTATTTGAA TTGCAACCAG GTGCTTCGGT GTTTGTCAAT 
GTGACTGATC CAAGCCAAGT GAGCCATGGC ACTGGCTTCA CGTCCTTTGG CTTACTCAAA 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000065.1
CDS	92..877
Translation	MIETYNQTSPRSAATGLPISMKIFMYLLTVFLITQMIGSALFAVYLHRRLDKIEDERNLHEDFVFMKTIQRCNTGERSLSLLNCEEIKSQFEGFVKDIMLNKEETKKENSFEMQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL

Target ORF information:

RefSeq Version	NM_000074.3
Organism	Homo sapiens(human)
Definition	Homo sapiens CD40 ligand (CD40LG), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000074.3

ORF Insert Sequence:

ATGATCGAAA CATACAACCA AACTTCTCCC CGATCTGCGG CCACTGGACT GCCCATCAGC 
ATGAAAATTT TTATGTATTT ACTTACTGTT TTTCTTATCA CCCAGATGAT TGGGTCAGCA 
CTTTTTGCTG TGTATCTTCA TAGAAGGTTG GACAAGATAG AAGATGAAAG GAATCTTCAT 
GAAGATTTTG TATTCATGAA AACGATACAG AGATGCAACA CAGGAGAAAG ATCCTTATCC 
TTACTGAACT GTGAGGAGAT TAAAAGCCAG TTTGAAGGCT TTGTGAAGGA TATAATGTTA 
AACAAAGAGG AGACGAAGAA AGAAAACAGC TTTGAAATGC AAAAAGGTGA TCAGAATCCT 
CAAATTGCGG CACATGTCAT AAGTGAGGCC AGCAGTAAAA CAACATCTGT GTTACAGTGG 
GCTGAAAAAG GATACTACAC CATGAGCAAC AACTTGGTAA CCCTGGAAAA TGGGAAACAG 
CTGACCGTTA AAAGACAAGG ACTCTATTAT ATCTATGCCC AAGTCACCTT CTGTTCCAAT 
CGGGAAGCTT CGAGTCAAGC TCCATTTATA GCCAGCCTCT GCCTAAAGTC CCCCGGTAGA 
TTCGAGAGAA TCTTACTCAG AGCTGCAAAT ACCCACAGTT CCGCCAAACC TTGCGGGCAA 
CAATCCATTC ACTTGGGAGG AGTATTTGAA TTGCAACCAG GTGCTTCGGT GTTTGTCAAT 
GTGACTGATC CAAGCCAAGT GAGCCATGGC ACTGGCTTCA CGTCCTTTGG CTTACTCAAA 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.