HELB cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HELB
Entrez Gene ID	92797
Full Name	DNA helicase B
Synonyms	DHB,hDHB
General protein information	Preferred Names DNA helicase B Names DNA helicase B helicase (DNA) B
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q14.3\|12q
Summary	This gene encodes a DNA-dependent ATPase which catalyzes the unwinding of DNA necessary for DNA replication, repair, recombination, and transcription. This gene is thought to function specifically during the S phase entry of the cell cycle. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016].
Disorder MIM:	614539

mRNA and Protein(s)

mRNA	Protein	Name
XM_005269234.2	XP_005269291.1	DNA helicase B isoform X1
NM_033647.4	NP_387467.2	DNA helicase B
XM_017020230.1	XP_016875719.1	DNA helicase B isoform X2
NM_001370285.1	NP_001357214.1	DNA helicase B

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	HELB	XP_850757.2
Rattus norvegicus (Norway rat)	Helb	XP_006241488.1
Xenopus tropicalis (tropical clawed frog)	helb	XP_002937585.2
Homo sapiens (human)	HELB	NP_387467.2
Bos taurus (cattle)	HELB	NP_001193111.1
Danio rerio (zebrafish)	helb	XP_001336777.3
Gallus gallus (chicken)	HELB	XP_416077.3
Pan troglodytes (chimpanzee)	HELB	XP_001162205.1
	HELB	XP_001117092.1
Mus musculus (house mouse)	Helb	NP_536694.2

Gene Ontology
Bibliography

Related articles in PubMed

HELB Is a Feedback Inhibitor of DNA End Resection.
Tk?? J, Xu G, Adhikary H, Young JTF, Gallo D, Escribano-D?az C, Krietsch J, Orthwein A, Munro M, Sol W, Al-Hakim A, Lin ZY, Jonkers J, Borst P, Brown GW, Gingras AC, Rottenberg S, Masson JY, Durocher D
Molecular cell61(3)405-418(2016 Feb)

Human DNA helicase B interacts with the replication initiation protein Cdc45 and facilitates Cdc45 binding onto chromatin.
Gerhardt J, Guler GD, Fanning E
Experimental cell research334(2)283-93(2015 Jun)

Human DNA helicase B functions in cellular homologous recombination and stimulates Rad51-mediated 5'-3' heteroduplex extension in vitro.
Liu H, Yan P, Fanning E
PloS one10(1)e0116852(2015)

Human DNA helicase B (HDHB) binds to replication protein A and facilitates cellular recovery from replication stress.
Guler GD, Liu H, Vaithiyalingam S, Arnett DR, Kremmer E, Chazin WJ, Fanning E
The Journal of biological chemistry287(9)6469-81(2012 Feb)

Cell cycle-dependent regulation of a human DNA helicase that localizes in DNA damage foci.
Gu J, Xia X, Yan P, Liu H, Podust VN, Reynolds AB, Fanning E
Molecular biology of the cell15(7)3320-32(2004 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Conclude that mammalian DNA end resection triggers its own inhibition via the recruitment of HELB.
Title: HELB Is a Feedback Inhibitor of DNA End Resection.

The depletion of HDHB from human cells diminishes Cdc45 association with chromatin, suggesting that HDHB may facilitate Cdc45 recruitment at G1/S in human cells.
Title: Human DNA helicase B interacts with the replication initiation protein Cdc45 and facilitates Cdc45 binding onto chromatin.

HDHB promotes homologous recombination in vivo and stimulates 5'-3' heteroduplex extension during Rad51-mediated strand exchange in vitro
Title: Human DNA helicase B functions in cellular homologous recombination and stimulates Rad51-mediated 5'-3' heteroduplex extension in vitro.

Replication stress-induced recruitment of HDHB to chromatin is independent of checkpoint signaling but correlates with the level of replication protein A (RPA) recruited to chromatin.
Title: Human DNA helicase B (HDHB) binds to replication protein A and facilitates cellular recovery from replication stress.

role of dominant-negative mutant in blocking onset of chromosomal DNA replication
Title: A dominant-negative mutant of human DNA helicase B blocks the onset of chromosomal DNA replication.

The following HELB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HELB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu84328	XM_017020230.1 Latest version!	Homo sapiens DNA helicase B (HELB), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu46986	NM_001370285.1 Latest version!	Homo sapiens DNA helicase B (HELB), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu46986	NM_033647.4 Latest version!	Homo sapiens DNA helicase B (HELB), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu46986	XM_005269234.2 Latest version!	Homo sapiens DNA helicase B (HELB), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HELB ( XM_017020230.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016875719.1 Homo sapiens DNA helicase B (HELB), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu84328
Clone ID Related Accession (Same CDS sequence)	XM_017020230.1
Accession Version	XM_017020230.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	DNA helicase B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGT CGAGTCCGTA CCTGCGCCAA CTTCAGGGAC CTCTGCTCCC ACCCAGGGAT 
CTGGTGGAGG AGGACGACGA CTACCTAAAC GACGACGTGG AGGAGGATGA AGAGTCCGTG 
TTCATCGACG CCGAGGAGCT CTGCAGTGGG GGCGTAAAGG CTGGCAGCCT CCCCGGGTGC 
CTCCGCGTTT CTATTTGTGA TGAAAACACA CAAGAGACAT GTAAAGTGTT TGGACGTTTT 
CCGATAACAG GTGCTTGGTG GAGAGTGAAG GTACAAGTAA AGCCTGTGGT GGGATCAAGG 
AGCTATCAAT ATCAAGTTCA AGGATTTCCG TCTTACTTTT TGCAGTCTGA TATGTCACCA 
CCAAATCAAA AACATATCTG TGCTCTCTTT CTTAAAGAGT GTGAGGTCTC CAGTGATGAT 
GTTAATAAAT TTTTAACATG GGTAAAGGAG GTATCAAACT ACAAAAACCT AAACTTTGAA 
AATCTTAGGG AAACACTAAG AACTTTCCAC AAGGAAACTG GAAGGAAAGA TCAAAAGCAG 
CCTACACAGA ATGGTCAGGA AGAGTTGTTC CTAGACAATG AGATGAGTCT TCCTCTGGAA 
AACACAATTC CATTTAGAAA TGTAATGACA GCTTTGCAGT TTCCGAAGAT AATGGAATTC 
CTTCCAGTTC TTCTGCCTCG ACACTTTAAA TGGATCATAG GGTCAGGTTC TAAAGAGATG 
TTGAAAGAGA TAGAAGAGAT TTTAGGTACA CATCCGTGGA AACTTGGATT TAGTAAAATA 
ACCTACAGAG AGTGGAAACT CCTGCGATGT GAGGCAAGTT GGATAGCATT TTGTCAGTGT 
GAGTCTCTTC TCCAGCTGAT GACTGATTTG GAGAAGAATG CATTAATAAT GTATTCCAGA 
CTGAAGCAGA TATGTAGAGA AGATGGGCAC ACATATGTTG AAGTGAATGA CTTAACTTTG 
ACATTGTCAA ATCATATGTC ATTTCATGCT GCTTCAGAGT CTCTGAAGTT TTTGAAGGAT 
ATTGGTGTGG TGACATATGA GAAGTCCTGT GTCTTCCCTT ATGACCTTTA CCATGCTGAA 
AGAGCCATCG CCTTTTCAAT TTGTGACCTG ATGAAGAAAC CTCCTTGGCA TTTATGTGTC 
GATGTCGAAA AGGTGCTTGC CTCTATTCAC ACCACAAAAC CTGAGAATTC AAGCGATGAT 
GCATTGAATG AGAGCAAACC TGATGAAGTA AGATTAGAAA ATCCTGTGGA TGTTGTGGAC 
ACACAGGACA ATGGTGACCA TATTTGGACT AATGGTGAAA ATGAAATTAA TGCAGAAATA 
AGTGAAGTTC AGCTGGATCA GGATCAGGTT GAAGTTCCAC TGGATCGGGA TCAGGTGGCT 
GCTTTGGAAA TGATTTGCTC CAATCCTGTG ACAGTCATAA GTGGGAAAGG TGGATGTGGG 
AAGACCACAA TCGTTAGCCG TCTTTTTAAG CATATAGAGC AGTTGGAAGA AAGAGAAGTA 
AAAAAAGCCT GTGAAGATTT TGAACAAGAC CAGAATGCTT CAGAAGAATG GATTACCTTT 
ACTGAGCAAA GTCAACTAGA GGCGGACAAG GCTATAGAAG TTTTGCTCAC AGCACCTACA 
GGGAAAGCAG CTGGCTTACT AAGACAGAAA ACTGGTCTTC ATGCCTACAC ACTGTGTCAG 
GTCAATTATA GCTTCTATTC ATGGACTCAA ACAATGATGA CCACAAACAA ACCATGGAAA 
TTTTCTTCGG TTAGAGTTCT GGTTGTGGAT GAAGGGAGTT TGGTATCTGT AGGAATCTTC 
AAATCGGTCT TAAATTTATT GTGTGAGCAC TCCAAACTTT CTAAGCTTAT TATCCTTGGT 
GACATTAGAC AGTTACCCAG TATTGAACCT GGTAACTTGC TGAAAGATCT TTTTGAGACT 
CTTAAGTCAA GAAATTGTGC TATTGAGCTA AAGACAAACC ATAGAGCAGA ATCTCAGCTC 
ATTGTGGACA ATGCTACAAG AATCTCAAGA CGCCAATTTC CAAAATTTGA TGCAGAACTA 
AATATCTCTG ATAATCCAAC ATTACCCATC TCAATTCAAG ATAAGACATT TATTTTTGTC 
AGGCTCCCAG AAGAGGATGC CAGTTCTCAG TCATCTAAAA CTAATCATCA CTCTTGTTTA 
TATTCTGCAG TTAAAACTTT ACTACAAGAA AATAACTTAC AAAATGCAAA AACATCACAA 
TTTATTGCAT TTAGAAGGCA AGACTGTGAT CTAATTAATG ACTGCTGCTG CAAACACTAC 
ACAGGCCACC TCACCAAAGA CCATCAGAGT AGACTTGTTT TTGGAATTGG TGATAAAATT 
TGTTGTACCA GGAATGCATA CCTCTCAGAC TTACTACCTG AAAATATCTC TGGAAGTCAG 
CAAAATAATG ATCTAGATGC CAGTAGTGAA GACTTTTCTG GATGTAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016875719.1
CDS	38..2488
Translation	MARSSPYLRQLQGPLLPPRDLVEEDDDYLNDDVEEDEESVFIDAEELCSGGVKAGSLPGCLRVSICDENTQETCKVFGRFPITGAWWRVKVQVKPVVGSRSYQYQVQGFPSYFLQSDMSPPNQKHICALFLKECEVSSDDVNKFLTWVKEVSNYKNLNFENLRETLRTFHKETGRKDQKQPTQNGQEELFLDNEMSLPLENTIPFRNVMTALQFPKIMEFLPVLLPRHFKWIIGSGSKEMLKEIEEILGTHPWKLGFSKITYREWKLLRCEASWIAFCQCESLLQLMTDLEKNALIMYSRLKQICREDGHTYVEVNDLTLTLSNHMSFHAASESLKFLKDIGVVTYEKSCVFPYDLYHAERAIAFSICDLMKKPPWHLCVDVEKVLASIHTTKPENSSDDALNESKPDEVRLENPVDVVDTQDNGDHIWTNGENEINAEISEVQLDQDQVEVPLDRDQVAALEMICSNPVTVISGKGGCGKTTIVSRLFKHIEQLEEREVKKACEDFEQDQNASEEWITFTEQSQLEADKAIEVLLTAPTGKAAGLLRQKTGLHAYTLCQVNYSFYSWTQTMMTTNKPWKFSSVRVLVVDEGSLVSVGIFKSVLNLLCEHSKLSKLIILGDIRQLPSIEPGNLLKDLFETLKSRNCAIELKTNHRAESQLIVDNATRISRRQFPKFDAELNISDNPTLPISIQDKTFIFVRLPEEDASSQSSKTNHHSCLYSAVKTLLQENNLQNAKTSQFIAFRRQDCDLINDCCCKHYTGHLTKDHQSRLVFGIGDKICCTRNAYLSDLLPENISGSQQNNDLDASSEDFSGCN

Target ORF information:

RefSeq Version	XM_017020230.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA helicase B (HELB), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020230.1

ORF Insert Sequence:

ATGGCCAGGT CGAGTCCGTA CCTGCGCCAA CTTCAGGGAC CTCTGCTCCC ACCCAGGGAT 
CTGGTGGAGG AGGACGACGA CTACCTAAAC GACGACGTGG AGGAGGATGA AGAGTCCGTG 
TTCATCGACG CCGAGGAGCT CTGCAGTGGG GGCGTAAAGG CTGGCAGCCT CCCCGGGTGC 
CTCCGCGTTT CTATTTGTGA TGAAAACACA CAAGAGACAT GTAAAGTGTT TGGACGTTTT 
CCGATAACAG GTGCTTGGTG GAGAGTGAAG GTACAAGTAA AGCCTGTGGT GGGATCAAGG 
AGCTATCAAT ATCAAGTTCA AGGATTTCCG TCTTACTTTT TGCAGTCTGA TATGTCACCA 
CCAAATCAAA AACATATCTG TGCTCTCTTT CTTAAAGAGT GTGAGGTCTC CAGTGATGAT 
GTTAATAAAT TTTTAACATG GGTAAAGGAG GTATCAAACT ACAAAAACCT AAACTTTGAA 
AATCTTAGGG AAACACTAAG AACTTTCCAC AAGGAAACTG GAAGGAAAGA TCAAAAGCAG 
CCTACACAGA ATGGTCAGGA AGAGTTGTTC CTAGACAATG AGATGAGTCT TCCTCTGGAA 
AACACAATTC CATTTAGAAA TGTAATGACA GCTTTGCAGT TTCCGAAGAT AATGGAATTC 
CTTCCAGTTC TTCTGCCTCG ACACTTTAAA TGGATCATAG GGTCAGGTTC TAAAGAGATG 
TTGAAAGAGA TAGAAGAGAT TTTAGGTACA CATCCGTGGA AACTTGGATT TAGTAAAATA 
ACCTACAGAG AGTGGAAACT CCTGCGATGT GAGGCAAGTT GGATAGCATT TTGTCAGTGT 
GAGTCTCTTC TCCAGCTGAT GACTGATTTG GAGAAGAATG CATTAATAAT GTATTCCAGA 
CTGAAGCAGA TATGTAGAGA AGATGGGCAC ACATATGTTG AAGTGAATGA CTTAACTTTG 
ACATTGTCAA ATCATATGTC ATTTCATGCT GCTTCAGAGT CTCTGAAGTT TTTGAAGGAT 
ATTGGTGTGG TGACATATGA GAAGTCCTGT GTCTTCCCTT ATGACCTTTA CCATGCTGAA 
AGAGCCATCG CCTTTTCAAT TTGTGACCTG ATGAAGAAAC CTCCTTGGCA TTTATGTGTC 
GATGTCGAAA AGGTGCTTGC CTCTATTCAC ACCACAAAAC CTGAGAATTC AAGCGATGAT 
GCATTGAATG AGAGCAAACC TGATGAAGTA AGATTAGAAA ATCCTGTGGA TGTTGTGGAC 
ACACAGGACA ATGGTGACCA TATTTGGACT AATGGTGAAA ATGAAATTAA TGCAGAAATA 
AGTGAAGTTC AGCTGGATCA GGATCAGGTT GAAGTTCCAC TGGATCGGGA TCAGGTGGCT 
GCTTTGGAAA TGATTTGCTC CAATCCTGTG ACAGTCATAA GTGGGAAAGG TGGATGTGGG 
AAGACCACAA TCGTTAGCCG TCTTTTTAAG CATATAGAGC AGTTGGAAGA AAGAGAAGTA 
AAAAAAGCCT GTGAAGATTT TGAACAAGAC CAGAATGCTT CAGAAGAATG GATTACCTTT 
ACTGAGCAAA GTCAACTAGA GGCGGACAAG GCTATAGAAG TTTTGCTCAC AGCACCTACA 
GGGAAAGCAG CTGGCTTACT AAGACAGAAA ACTGGTCTTC ATGCCTACAC ACTGTGTCAG 
GTCAATTATA GCTTCTATTC ATGGACTCAA ACAATGATGA CCACAAACAA ACCATGGAAA 
TTTTCTTCGG TTAGAGTTCT GGTTGTGGAT GAAGGGAGTT TGGTATCTGT AGGAATCTTC 
AAATCGGTCT TAAATTTATT GTGTGAGCAC TCCAAACTTT CTAAGCTTAT TATCCTTGGT 
GACATTAGAC AGTTACCCAG TATTGAACCT GGTAACTTGC TGAAAGATCT TTTTGAGACT 
CTTAAGTCAA GAAATTGTGC TATTGAGCTA AAGACAAACC ATAGAGCAGA ATCTCAGCTC 
ATTGTGGACA ATGCTACAAG AATCTCAAGA CGCCAATTTC CAAAATTTGA TGCAGAACTA 
AATATCTCTG ATAATCCAAC ATTACCCATC TCAATTCAAG ATAAGACATT TATTTTTGTC 
AGGCTCCCAG AAGAGGATGC CAGTTCTCAG TCATCTAAAA CTAATCATCA CTCTTGTTTA 
TATTCTGCAG TTAAAACTTT ACTACAAGAA AATAACTTAC AAAATGCAAA AACATCACAA 
TTTATTGCAT TTAGAAGGCA AGACTGTGAT CTAATTAATG ACTGCTGCTG CAAACACTAC 
ACAGGCCACC TCACCAAAGA CCATCAGAGT AGACTTGTTT TTGGAATTGG TGATAAAATT 
TGTTGTACCA GGAATGCATA CCTCTCAGAC TTACTACCTG AAAATATCTC TGGAAGTCAG 
CAAAATAATG ATCTAGATGC CAGTAGTGAA GACTTTTCTG GATGTAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HELB ( NM_033647.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_387467.2 Homo sapiens DNA helicase B (HELB), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu46986
Clone ID Related Accession (Same CDS sequence)	XM_005269234.2 , NM_033647.4 , NM_001370285.1
Accession Version	NM_033647.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Homo sapiens(human)
Product	DNA helicase B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC078889.20, BC143411.1, AK290894.1 and AF319995.1. On Feb 4, 2016 this sequence version replaced NM_033647.3. Transcript Variant: This variant (1) encodes the protein. ##Evidence-Data-START## Transcript exon combination :: AK290894.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMN01820689 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## NMD candidate :: PMID: 22194613 ##RefSeq-Attributes-END##

ATGGCCAGGT CGAGTCCGTA CCTGCGCCAA CTTCAGGGAC CTCTGCTCCC ACCCAGGGAT 
CTGGTGGAGG AGGACGACGA CTACCTAAAC GACGACGTGG AGGAGGATGA AGAGTCCGTG 
TTCATCGACG CCGAGGAGCT CTGCAGTGGG GGCGTAAAGG CTGGCAGCCT CCCCGGGTGC 
CTCCGCGTTT CTATTTGTGA TGAAAACACA CAAGAGACAT GTAAAGTGTT TGGACGTTTT 
CCGATAACAG GTGCTTGGTG GAGAGTGAAG GTACAAGTAA AGCCTGTGGT GGGATCAAGG 
AGCTATCAAT ATCAAGTTCA AGGATTTCCG TCTTACTTTT TGCAGTCTGA TATGTCACCA 
CCAAATCAAA AACATATCTG TGCTCTCTTT CTTAAAGAGT GTGAGGTCTC CAGTGATGAT 
GTTAATAAAT TTTTAACATG GGTAAAGGAG GTATCAAACT ACAAAAACCT AAACTTTGAA 
AATCTTAGGG AAACACTAAG AACTTTCCAC AAGGAAACTG GAAGGAAAGA TCAAAAGCAG 
CCTACACAGA ATGGTCAGGA AGAGTTGTTC CTAGACAATG AGATGAGTCT TCCTCTGGAA 
AACACAATTC CATTTAGAAA TGTAATGACA GCTTTGCAGT TTCCGAAGAT AATGGAATTC 
CTTCCAGTTC TTCTGCCTCG ACACTTTAAA TGGATCATAG GGTCAGGTTC TAAAGAGATG 
TTGAAAGAGA TAGAAGAGAT TTTAGGTACA CATCCGTGGA AACTTGGATT TAGTAAAATA 
ACCTACAGAG AGTGGAAACT CCTGCGATGT GAGGCAAGTT GGATAGCATT TTGTCAGTGT 
GAGTCTCTTC TCCAGCTGAT GACTGATTTG GAGAAGAATG CATTAATAAT GTATTCCAGA 
CTGAAGCAGA TATGTAGAGA AGATGGGCAC ACATATGTTG AAGTGAATGA CTTAACTTTG 
ACATTGTCAA ATCATATGTC ATTTCATGCT GCTTCAGAGT CTCTGAAGTT TTTGAAGGAT 
ATTGGTGTGG TGACATATGA GAAGTCCTGT GTCTTCCCTT ATGACCTTTA CCATGCTGAA 
AGAGCCATCG CCTTTTCAAT TTGTGACCTG ATGAAGAAAC CTCCTTGGCA TTTATGTGTC 
GATGTCGAAA AGGTGCTTGC CTCTATTCAC ACCACAAAAC CTGAGAATTC AAGCGATGAT 
GCATTGAATG AGAGCAAACC TGATGAAGTA AGATTAGAAA ATCCTGTGGA TGTTGTGGAC 
ACACAGGACA ATGGTGACCA TATTTGGACT AATGGTGAAA ATGAAATTAA TGCAGAAATA 
AGTGAAGTTC AGCTGGATCA GGATCAGGTT GAAGTTCCAC TGGATCGGGA TCAGGTGGCT 
GCTTTGGAAA TGATTTGCTC CAATCCTGTG ACAGTCATAA GTGGGAAAGG TGGATGTGGG 
AAGACCACAA TCGTTAGCCG TCTTTTTAAG CATATAGAGC AGTTGGAAGA AAGAGAAGTA 
AAAAAAGCCT GTGAAGATTT TGAACAAGAC CAGAATGCTT CAGAAGAATG GATTACCTTT 
ACTGAGCAAA GTCAACTAGA GGCGGACAAG GCTATAGAAG TTTTGCTCAC AGCACCTACA 
GGGAAAGCAG CTGGCTTACT AAGACAGAAA ACTGGTCTTC ATGCCTACAC ACTGTGTCAG 
GTCAATTATA GCTTCTATTC ATGGACTCAA ACAATGATGA CCACAAACAA ACCATGGAAA 
TTTTCTTCGG TTAGAGTTCT GGTTGTGGAT GAAGGGAGTT TGGTATCTGT AGGAATCTTC 
AAATCGGTCT TAAATTTATT GTGTGAGCAC TCCAAACTTT CTAAGCTTAT TATCCTTGGT 
GACATTAGAC AGTTACCCAG TATTGAACCT GGTAACTTGC TGAAAGATCT TTTTGAGACT 
CTTAAGTCAA GAAATTGTGC TATTGAGCTA AAGACAAACC ATAGAGCAGA ATCTCAGCTC 
ATTGTGGACA ATGCTACAAG AATCTCAAGA CGCCAATTTC CAAAATTTGA TGCAGAACTA 
AATATCTCTG ATAATCCAAC ATTACCCATC TCAATTCAAG ATAAGACATT TATTTTTGTC 
AGGCTCCCAG AAGAGGATGC CAGTTCTCAG TCATCTAAAA CTAATCATCA CTCTTGTTTA 
TATTCTGCAG TTAAAACTTT ACTACAAGAA AATAACTTAC AAAATGCAAA AACATCACAA 
TTTATTGCAT TTAGAAGGCA AGACTGTGAT CTAATTAATG ACTGCTGCTG CAAACACTAC 
ACAGGCCACC TCACCAAAGA CCATCAGAGT AGACTTGTTT TTGGAATTGG TGATAAAATT 
TGTTGTACCA GGAATGCATA CCTCTCAGAC TTACTACCTG AAAATATCTC TGGAAGTCAG 
CAAAATAATG ATCTAGATGC CAGTAGTGAA GACTTTTCTG GTACGCTTCC TGATTTTGCT 
AAAAATAAGC GTGACTTTGA AAGTAACGTT CGACTGTGCA ATGGAGAGAT ATTTTTCATA 
ACAAATGATG TAACTGATGT AACTTTTGGA AAGAGAAGAT CTTTGACCAT TAATAATATG 
GCTGGCCTGG AAGTAACTGT GGATTTTAAG AAACTAATGA AATATTGTCG CATAAAACAT 
GCATGGGCAA GAACTATTCA CACTTTTCAG GGGTCCGAGG AGCAAACAGT TGTCTATGTG 
GTGGGGAAGG CGGGCCGCCA GCACTGGCAG CATGTCTACA CCGCCGTGAC CAGGGGCCGC 
TGCCGAGTGT ATGTGATTGC AGAGGAGTCT CAGCTCCGGA ATGCCATTAT GAAAAACAGT 
TTTCCTAGAA AAACTCGTTT GAAACATTTC TTGCAAAGTA AGCTCTCCTC TAGCGGCGCA 
CCTCCAGCAG ATTTTCCGTC CCCACGGAAG AGCTCTGGAG ACAGTGGAGG ACCCAGCACA 
CCGTCAGCAT CTCCACTCCC TGTAGTCACA GACCACGCCA TGACAAATGA TGTCACCTGG 
AGCGAGGCCT CTTCGCCTGA TGAGAGGACA CTCACCTTTG CTGAAAGATG GCAATTATCT 
TCACCTGATG GAGTAGATAC AGATGATGAT TTACCAAAAT CGCGAGCATC CAAAAGAACC 
TGTGGTGTGA ATGATGATGA AAGTCCAAGC AAAATTTTTA TGGTGGGAGA ATCTCCACAA 
GTGTCTTCCA GACTTCAGAA TTTGAGACTG AATAATTTAA TTCCCAGGCA ACTTTTCAAG 
CCCACCGATA ATCAAGAAAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_387467.2
CDS	60..3323
Translation	MARSSPYLRQLQGPLLPPRDLVEEDDDYLNDDVEEDEESVFIDAEELCSGGVKAGSLPGCLRVSICDENTQETCKVFGRFPITGAWWRVKVQVKPVVGSRSYQYQVQGFPSYFLQSDMSPPNQKHICALFLKECEVSSDDVNKFLTWVKEVSNYKNLNFENLRETLRTFHKETGRKDQKQPTQNGQEELFLDNEMSLPLENTIPFRNVMTALQFPKIMEFLPVLLPRHFKWIIGSGSKEMLKEIEEILGTHPWKLGFSKITYREWKLLRCEASWIAFCQCESLLQLMTDLEKNALIMYSRLKQICREDGHTYVEVNDLTLTLSNHMSFHAASESLKFLKDIGVVTYEKSCVFPYDLYHAERAIAFSICDLMKKPPWHLCVDVEKVLASIHTTKPENSSDDALNESKPDEVRLENPVDVVDTQDNGDHIWTNGENEINAEISEVQLDQDQVEVPLDRDQVAALEMICSNPVTVISGKGGCGKTTIVSRLFKHIEQLEEREVKKACEDFEQDQNASEEWITFTEQSQLEADKAIEVLLTAPTGKAAGLLRQKTGLHAYTLCQVNYSFYSWTQTMMTTNKPWKFSSVRVLVVDEGSLVSVGIFKSVLNLLCEHSKLSKLIILGDIRQLPSIEPGNLLKDLFETLKSRNCAIELKTNHRAESQLIVDNATRISRRQFPKFDAELNISDNPTLPISIQDKTFIFVRLPEEDASSQSSKTNHHSCLYSAVKTLLQENNLQNAKTSQFIAFRRQDCDLINDCCCKHYTGHLTKDHQSRLVFGIGDKICCTRNAYLSDLLPENISGSQQNNDLDASSEDFSGTLPDFAKNKRDFESNVRLCNGEIFFITNDVTDVTFGKRRSLTINNMAGLEVTVDFKKLMKYCRIKHAWARTIHTFQGSEEQTVVYVVGKAGRQHWQHVYTAVTRGRCRVYVIAEESQLRNAIMKNSFPRKTRLKHFLQSKLSSSGAPPADFPSPRKSSGDSGGPSTPSASPLPVVTDHAMTNDVTWSEASSPDERTLTFAERWQLSSPDGVDTDDDLPKSRASKRTCGVNDDESPSKIFMVGESPQVSSRLQNLRLNNLIPRQLFKPTDNQET

Target ORF information:

RefSeq Version	NM_033647.4
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA helicase B (HELB), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033647.4

ORF Insert Sequence:

ATGGCCAGGT CGAGTCCGTA CCTGCGCCAA CTTCAGGGAC CTCTGCTCCC ACCCAGGGAT 
CTGGTGGAGG AGGACGACGA CTACCTAAAC GACGACGTGG AGGAGGATGA AGAGTCCGTG 
TTCATCGACG CCGAGGAGCT CTGCAGTGGG GGCGTAAAGG CTGGCAGCCT CCCCGGGTGC 
CTCCGCGTTT CTATTTGTGA TGAAAACACA CAAGAGACAT GTAAAGTGTT TGGACGTTTT 
CCGATAACAG GTGCTTGGTG GAGAGTGAAG GTACAAGTAA AGCCTGTGGT GGGATCAAGG 
AGCTATCAAT ATCAAGTTCA AGGATTTCCG TCTTACTTTT TGCAGTCTGA TATGTCACCA 
CCAAATCAAA AACATATCTG TGCTCTCTTT CTTAAAGAGT GTGAGGTCTC CAGTGATGAT 
GTTAATAAAT TTTTAACATG GGTAAAGGAG GTATCAAACT ACAAAAACCT AAACTTTGAA 
AATCTTAGGG AAACACTAAG AACTTTCCAC AAGGAAACTG GAAGGAAAGA TCAAAAGCAG 
CCTACACAGA ATGGTCAGGA AGAGTTGTTC CTAGACAATG AGATGAGTCT TCCTCTGGAA 
AACACAATTC CATTTAGAAA TGTAATGACA GCTTTGCAGT TTCCGAAGAT AATGGAATTC 
CTTCCAGTTC TTCTGCCTCG ACACTTTAAA TGGATCATAG GGTCAGGTTC TAAAGAGATG 
TTGAAAGAGA TAGAAGAGAT TTTAGGTACA CATCCGTGGA AACTTGGATT TAGTAAAATA 
ACCTACAGAG AGTGGAAACT CCTGCGATGT GAGGCAAGTT GGATAGCATT TTGTCAGTGT 
GAGTCTCTTC TCCAGCTGAT GACTGATTTG GAGAAGAATG CATTAATAAT GTATTCCAGA 
CTGAAGCAGA TATGTAGAGA AGATGGGCAC ACATATGTTG AAGTGAATGA CTTAACTTTG 
ACATTGTCAA ATCATATGTC ATTTCATGCT GCTTCAGAGT CTCTGAAGTT TTTGAAGGAT 
ATTGGTGTGG TGACATATGA GAAGTCCTGT GTCTTCCCTT ATGACCTTTA CCATGCTGAA 
AGAGCCATCG CCTTTTCAAT TTGTGACCTG ATGAAGAAAC CTCCTTGGCA TTTATGTGTC 
GATGTCGAAA AGGTGCTTGC CTCTATTCAC ACCACAAAAC CTGAGAATTC AAGCGATGAT 
GCATTGAATG AGAGCAAACC TGATGAAGTA AGATTAGAAA ATCCTGTGGA TGTTGTGGAC 
ACACAGGACA ATGGTGACCA TATTTGGACT AATGGTGAAA ATGAAATTAA TGCAGAAATA 
AGTGAAGTTC AGCTGGATCA GGATCAGGTT GAAGTTCCAC TGGATCGGGA TCAGGTGGCT 
GCTTTGGAAA TGATTTGCTC CAATCCTGTG ACAGTCATAA GTGGGAAAGG TGGATGTGGG 
AAGACCACAA TCGTTAGCCG TCTTTTTAAG CATATAGAGC AGTTGGAAGA AAGAGAAGTA 
AAAAAAGCCT GTGAAGATTT TGAACAAGAC CAGAATGCTT CAGAAGAATG GATTACCTTT 
ACTGAGCAAA GTCAACTAGA GGCGGACAAG GCTATAGAAG TTTTGCTCAC AGCACCTACA 
GGGAAAGCAG CTGGCTTACT AAGACAGAAA ACTGGTCTTC ATGCCTACAC ACTGTGTCAG 
GTCAATTATA GCTTCTATTC ATGGACTCAA ACAATGATGA CCACAAACAA ACCATGGAAA 
TTTTCTTCGG TTAGAGTTCT GGTTGTGGAT GAAGGGAGTT TGGTATCTGT AGGAATCTTC 
AAATCGGTCT TAAATTTATT GTGTGAGCAC TCCAAACTTT CTAAGCTTAT TATCCTTGGT 
GACATTAGAC AGTTACCCAG TATTGAACCT GGTAACTTGC TGAAAGATCT TTTTGAGACT 
CTTAAGTCAA GAAATTGTGC TATTGAGCTA AAGACAAACC ATAGAGCAGA ATCTCAGCTC 
ATTGTGGACA ATGCTACAAG AATCTCAAGA CGCCAATTTC CAAAATTTGA TGCAGAACTA 
AATATCTCTG ATAATCCAAC ATTACCCATC TCAATTCAAG ATAAGACATT TATTTTTGTC 
AGGCTCCCAG AAGAGGATGC CAGTTCTCAG TCATCTAAAA CTAATCATCA CTCTTGTTTA 
TATTCTGCAG TTAAAACTTT ACTACAAGAA AATAACTTAC AAAATGCAAA AACATCACAA 
TTTATTGCAT TTAGAAGGCA AGACTGTGAT CTAATTAATG ACTGCTGCTG CAAACACTAC 
ACAGGCCACC TCACCAAAGA CCATCAGAGT AGACTTGTTT TTGGAATTGG TGATAAAATT 
TGTTGTACCA GGAATGCATA CCTCTCAGAC TTACTACCTG AAAATATCTC TGGAAGTCAG 
CAAAATAATG ATCTAGATGC CAGTAGTGAA GACTTTTCTG GTACGCTTCC TGATTTTGCT 
AAAAATAAGC GTGACTTTGA AAGTAACGTT CGACTGTGCA ATGGAGAGAT ATTTTTCATA 
ACAAATGATG TAACTGATGT AACTTTTGGA AAGAGAAGAT CTTTGACCAT TAATAATATG 
GCTGGCCTGG AAGTAACTGT GGATTTTAAG AAACTAATGA AATATTGTCG CATAAAACAT 
GCATGGGCAA GAACTATTCA CACTTTTCAG GGGTCCGAGG AGCAAACAGT TGTCTATGTG 
GTGGGGAAGG CGGGCCGCCA GCACTGGCAG CATGTCTACA CCGCCGTGAC CAGGGGCCGC 
TGCCGAGTGT ATGTGATTGC AGAGGAGTCT CAGCTCCGGA ATGCCATTAT GAAAAACAGT 
TTTCCTAGAA AAACTCGTTT GAAACATTTC TTGCAAAGTA AGCTCTCCTC TAGCGGCGCA 
CCTCCAGCAG ATTTTCCGTC CCCACGGAAG AGCTCTGGAG ACAGTGGAGG ACCCAGCACA 
CCGTCAGCAT CTCCACTCCC TGTAGTCACA GACCACGCCA TGACAAATGA TGTCACCTGG 
AGCGAGGCCT CTTCGCCTGA TGAGAGGACA CTCACCTTTG CTGAAAGATG GCAATTATCT 
TCACCTGATG GAGTAGATAC AGATGATGAT TTACCAAAAT CGCGAGCATC CAAAAGAACC 
TGTGGTGTGA ATGATGATGA AAGTCCAAGC AAAATTTTTA TGGTGGGAGA ATCTCCACAA 
GTGTCTTCCA GACTTCAGAA TTTGAGACTG AATAATTTAA TTCCCAGGCA ACTTTTCAAG 
CCCACCGATA ATCAAGAAAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HELB ( NM_001370285.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001357214.1 Homo sapiens DNA helicase B (HELB), transcript variant 4, mRNA.

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CloneID	OHu46986
Clone ID Related Accession (Same CDS sequence)	XM_005269234.2 , NM_033647.4 , NM_001370285.1
Accession Version	NM_001370285.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	DNA helicase B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC078889.20. On May 4, 2019 this sequence version replaced XM_005269234.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.41586.1, AF319995.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END##

ATGGCCAGGT CGAGTCCGTA CCTGCGCCAA CTTCAGGGAC CTCTGCTCCC ACCCAGGGAT 
CTGGTGGAGG AGGACGACGA CTACCTAAAC GACGACGTGG AGGAGGATGA AGAGTCCGTG 
TTCATCGACG CCGAGGAGCT CTGCAGTGGG GGCGTAAAGG CTGGCAGCCT CCCCGGGTGC 
CTCCGCGTTT CTATTTGTGA TGAAAACACA CAAGAGACAT GTAAAGTGTT TGGACGTTTT 
CCGATAACAG GTGCTTGGTG GAGAGTGAAG GTACAAGTAA AGCCTGTGGT GGGATCAAGG 
AGCTATCAAT ATCAAGTTCA AGGATTTCCG TCTTACTTTT TGCAGTCTGA TATGTCACCA 
CCAAATCAAA AACATATCTG TGCTCTCTTT CTTAAAGAGT GTGAGGTCTC CAGTGATGAT 
GTTAATAAAT TTTTAACATG GGTAAAGGAG GTATCAAACT ACAAAAACCT AAACTTTGAA 
AATCTTAGGG AAACACTAAG AACTTTCCAC AAGGAAACTG GAAGGAAAGA TCAAAAGCAG 
CCTACACAGA ATGGTCAGGA AGAGTTGTTC CTAGACAATG AGATGAGTCT TCCTCTGGAA 
AACACAATTC CATTTAGAAA TGTAATGACA GCTTTGCAGT TTCCGAAGAT AATGGAATTC 
CTTCCAGTTC TTCTGCCTCG ACACTTTAAA TGGATCATAG GGTCAGGTTC TAAAGAGATG 
TTGAAAGAGA TAGAAGAGAT TTTAGGTACA CATCCGTGGA AACTTGGATT TAGTAAAATA 
ACCTACAGAG AGTGGAAACT CCTGCGATGT GAGGCAAGTT GGATAGCATT TTGTCAGTGT 
GAGTCTCTTC TCCAGCTGAT GACTGATTTG GAGAAGAATG CATTAATAAT GTATTCCAGA 
CTGAAGCAGA TATGTAGAGA AGATGGGCAC ACATATGTTG AAGTGAATGA CTTAACTTTG 
ACATTGTCAA ATCATATGTC ATTTCATGCT GCTTCAGAGT CTCTGAAGTT TTTGAAGGAT 
ATTGGTGTGG TGACATATGA GAAGTCCTGT GTCTTCCCTT ATGACCTTTA CCATGCTGAA 
AGAGCCATCG CCTTTTCAAT TTGTGACCTG ATGAAGAAAC CTCCTTGGCA TTTATGTGTC 
GATGTCGAAA AGGTGCTTGC CTCTATTCAC ACCACAAAAC CTGAGAATTC AAGCGATGAT 
GCATTGAATG AGAGCAAACC TGATGAAGTA AGATTAGAAA ATCCTGTGGA TGTTGTGGAC 
ACACAGGACA ATGGTGACCA TATTTGGACT AATGGTGAAA ATGAAATTAA TGCAGAAATA 
AGTGAAGTTC AGCTGGATCA GGATCAGGTT GAAGTTCCAC TGGATCGGGA TCAGGTGGCT 
GCTTTGGAAA TGATTTGCTC CAATCCTGTG ACAGTCATAA GTGGGAAAGG TGGATGTGGG 
AAGACCACAA TCGTTAGCCG TCTTTTTAAG CATATAGAGC AGTTGGAAGA AAGAGAAGTA 
AAAAAAGCCT GTGAAGATTT TGAACAAGAC CAGAATGCTT CAGAAGAATG GATTACCTTT 
ACTGAGCAAA GTCAACTAGA GGCGGACAAG GCTATAGAAG TTTTGCTCAC AGCACCTACA 
GGGAAAGCAG CTGGCTTACT AAGACAGAAA ACTGGTCTTC ATGCCTACAC ACTGTGTCAG 
GTCAATTATA GCTTCTATTC ATGGACTCAA ACAATGATGA CCACAAACAA ACCATGGAAA 
TTTTCTTCGG TTAGAGTTCT GGTTGTGGAT GAAGGGAGTT TGGTATCTGT AGGAATCTTC 
AAATCGGTCT TAAATTTATT GTGTGAGCAC TCCAAACTTT CTAAGCTTAT TATCCTTGGT 
GACATTAGAC AGTTACCCAG TATTGAACCT GGTAACTTGC TGAAAGATCT TTTTGAGACT 
CTTAAGTCAA GAAATTGTGC TATTGAGCTA AAGACAAACC ATAGAGCAGA ATCTCAGCTC 
ATTGTGGACA ATGCTACAAG AATCTCAAGA CGCCAATTTC CAAAATTTGA TGCAGAACTA 
AATATCTCTG ATAATCCAAC ATTACCCATC TCAATTCAAG ATAAGACATT TATTTTTGTC 
AGGCTCCCAG AAGAGGATGC CAGTTCTCAG TCATCTAAAA CTAATCATCA CTCTTGTTTA 
TATTCTGCAG TTAAAACTTT ACTACAAGAA AATAACTTAC AAAATGCAAA AACATCACAA 
TTTATTGCAT TTAGAAGGCA AGACTGTGAT CTAATTAATG ACTGCTGCTG CAAACACTAC 
ACAGGCCACC TCACCAAAGA CCATCAGAGT AGACTTGTTT TTGGAATTGG TGATAAAATT 
TGTTGTACCA GGAATGCATA CCTCTCAGAC TTACTACCTG AAAATATCTC TGGAAGTCAG 
CAAAATAATG ATCTAGATGC CAGTAGTGAA GACTTTTCTG GTACGCTTCC TGATTTTGCT 
AAAAATAAGC GTGACTTTGA AAGTAACGTT CGACTGTGCA ATGGAGAGAT ATTTTTCATA 
ACAAATGATG TAACTGATGT AACTTTTGGA AAGAGAAGAT CTTTGACCAT TAATAATATG 
GCTGGCCTGG AAGTAACTGT GGATTTTAAG AAACTAATGA AATATTGTCG CATAAAACAT 
GCATGGGCAA GAACTATTCA CACTTTTCAG GGGTCCGAGG AGCAAACAGT TGTCTATGTG 
GTGGGGAAGG CGGGCCGCCA GCACTGGCAG CATGTCTACA CCGCCGTGAC CAGGGGCCGC 
TGCCGAGTGT ATGTGATTGC AGAGGAGTCT CAGCTCCGGA ATGCCATTAT GAAAAACAGT 
TTTCCTAGAA AAACTCGTTT GAAACATTTC TTGCAAAGTA AGCTCTCCTC TAGCGGCGCA 
CCTCCAGCAG ATTTTCCGTC CCCACGGAAG AGCTCTGGAG ACAGTGGAGG ACCCAGCACA 
CCGTCAGCAT CTCCACTCCC TGTAGTCACA GACCACGCCA TGACAAATGA TGTCACCTGG 
AGCGAGGCCT CTTCGCCTGA TGAGAGGACA CTCACCTTTG CTGAAAGATG GCAATTATCT 
TCACCTGATG GAGTAGATAC AGATGATGAT TTACCAAAAT CGCGAGCATC CAAAAGAACC 
TGTGGTGTGA ATGATGATGA AAGTCCAAGC AAAATTTTTA TGGTGGGAGA ATCTCCACAA 
GTGTCTTCCA GACTTCAGAA TTTGAGACTG AATAATTTAA TTCCCAGGCA ACTTTTCAAG 
CCCACCGATA ATCAAGAAAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001357214.1
CDS	112..3375
Translation	MARSSPYLRQLQGPLLPPRDLVEEDDDYLNDDVEEDEESVFIDAEELCSGGVKAGSLPGCLRVSICDENTQETCKVFGRFPITGAWWRVKVQVKPVVGSRSYQYQVQGFPSYFLQSDMSPPNQKHICALFLKECEVSSDDVNKFLTWVKEVSNYKNLNFENLRETLRTFHKETGRKDQKQPTQNGQEELFLDNEMSLPLENTIPFRNVMTALQFPKIMEFLPVLLPRHFKWIIGSGSKEMLKEIEEILGTHPWKLGFSKITYREWKLLRCEASWIAFCQCESLLQLMTDLEKNALIMYSRLKQICREDGHTYVEVNDLTLTLSNHMSFHAASESLKFLKDIGVVTYEKSCVFPYDLYHAERAIAFSICDLMKKPPWHLCVDVEKVLASIHTTKPENSSDDALNESKPDEVRLENPVDVVDTQDNGDHIWTNGENEINAEISEVQLDQDQVEVPLDRDQVAALEMICSNPVTVISGKGGCGKTTIVSRLFKHIEQLEEREVKKACEDFEQDQNASEEWITFTEQSQLEADKAIEVLLTAPTGKAAGLLRQKTGLHAYTLCQVNYSFYSWTQTMMTTNKPWKFSSVRVLVVDEGSLVSVGIFKSVLNLLCEHSKLSKLIILGDIRQLPSIEPGNLLKDLFETLKSRNCAIELKTNHRAESQLIVDNATRISRRQFPKFDAELNISDNPTLPISIQDKTFIFVRLPEEDASSQSSKTNHHSCLYSAVKTLLQENNLQNAKTSQFIAFRRQDCDLINDCCCKHYTGHLTKDHQSRLVFGIGDKICCTRNAYLSDLLPENISGSQQNNDLDASSEDFSGTLPDFAKNKRDFESNVRLCNGEIFFITNDVTDVTFGKRRSLTINNMAGLEVTVDFKKLMKYCRIKHAWARTIHTFQGSEEQTVVYVVGKAGRQHWQHVYTAVTRGRCRVYVIAEESQLRNAIMKNSFPRKTRLKHFLQSKLSSSGAPPADFPSPRKSSGDSGGPSTPSASPLPVVTDHAMTNDVTWSEASSPDERTLTFAERWQLSSPDGVDTDDDLPKSRASKRTCGVNDDESPSKIFMVGESPQVSSRLQNLRLNNLIPRQLFKPTDNQET

Target ORF information:

RefSeq Version	NM_001370285.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA helicase B (HELB), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001370285.1

ORF Insert Sequence:

ATGGCCAGGT CGAGTCCGTA CCTGCGCCAA CTTCAGGGAC CTCTGCTCCC ACCCAGGGAT 
CTGGTGGAGG AGGACGACGA CTACCTAAAC GACGACGTGG AGGAGGATGA AGAGTCCGTG 
TTCATCGACG CCGAGGAGCT CTGCAGTGGG GGCGTAAAGG CTGGCAGCCT CCCCGGGTGC 
CTCCGCGTTT CTATTTGTGA TGAAAACACA CAAGAGACAT GTAAAGTGTT TGGACGTTTT 
CCGATAACAG GTGCTTGGTG GAGAGTGAAG GTACAAGTAA AGCCTGTGGT GGGATCAAGG 
AGCTATCAAT ATCAAGTTCA AGGATTTCCG TCTTACTTTT TGCAGTCTGA TATGTCACCA 
CCAAATCAAA AACATATCTG TGCTCTCTTT CTTAAAGAGT GTGAGGTCTC CAGTGATGAT 
GTTAATAAAT TTTTAACATG GGTAAAGGAG GTATCAAACT ACAAAAACCT AAACTTTGAA 
AATCTTAGGG AAACACTAAG AACTTTCCAC AAGGAAACTG GAAGGAAAGA TCAAAAGCAG 
CCTACACAGA ATGGTCAGGA AGAGTTGTTC CTAGACAATG AGATGAGTCT TCCTCTGGAA 
AACACAATTC CATTTAGAAA TGTAATGACA GCTTTGCAGT TTCCGAAGAT AATGGAATTC 
CTTCCAGTTC TTCTGCCTCG ACACTTTAAA TGGATCATAG GGTCAGGTTC TAAAGAGATG 
TTGAAAGAGA TAGAAGAGAT TTTAGGTACA CATCCGTGGA AACTTGGATT TAGTAAAATA 
ACCTACAGAG AGTGGAAACT CCTGCGATGT GAGGCAAGTT GGATAGCATT TTGTCAGTGT 
GAGTCTCTTC TCCAGCTGAT GACTGATTTG GAGAAGAATG CATTAATAAT GTATTCCAGA 
CTGAAGCAGA TATGTAGAGA AGATGGGCAC ACATATGTTG AAGTGAATGA CTTAACTTTG 
ACATTGTCAA ATCATATGTC ATTTCATGCT GCTTCAGAGT CTCTGAAGTT TTTGAAGGAT 
ATTGGTGTGG TGACATATGA GAAGTCCTGT GTCTTCCCTT ATGACCTTTA CCATGCTGAA 
AGAGCCATCG CCTTTTCAAT TTGTGACCTG ATGAAGAAAC CTCCTTGGCA TTTATGTGTC 
GATGTCGAAA AGGTGCTTGC CTCTATTCAC ACCACAAAAC CTGAGAATTC AAGCGATGAT 
GCATTGAATG AGAGCAAACC TGATGAAGTA AGATTAGAAA ATCCTGTGGA TGTTGTGGAC 
ACACAGGACA ATGGTGACCA TATTTGGACT AATGGTGAAA ATGAAATTAA TGCAGAAATA 
AGTGAAGTTC AGCTGGATCA GGATCAGGTT GAAGTTCCAC TGGATCGGGA TCAGGTGGCT 
GCTTTGGAAA TGATTTGCTC CAATCCTGTG ACAGTCATAA GTGGGAAAGG TGGATGTGGG 
AAGACCACAA TCGTTAGCCG TCTTTTTAAG CATATAGAGC AGTTGGAAGA AAGAGAAGTA 
AAAAAAGCCT GTGAAGATTT TGAACAAGAC CAGAATGCTT CAGAAGAATG GATTACCTTT 
ACTGAGCAAA GTCAACTAGA GGCGGACAAG GCTATAGAAG TTTTGCTCAC AGCACCTACA 
GGGAAAGCAG CTGGCTTACT AAGACAGAAA ACTGGTCTTC ATGCCTACAC ACTGTGTCAG 
GTCAATTATA GCTTCTATTC ATGGACTCAA ACAATGATGA CCACAAACAA ACCATGGAAA 
TTTTCTTCGG TTAGAGTTCT GGTTGTGGAT GAAGGGAGTT TGGTATCTGT AGGAATCTTC 
AAATCGGTCT TAAATTTATT GTGTGAGCAC TCCAAACTTT CTAAGCTTAT TATCCTTGGT 
GACATTAGAC AGTTACCCAG TATTGAACCT GGTAACTTGC TGAAAGATCT TTTTGAGACT 
CTTAAGTCAA GAAATTGTGC TATTGAGCTA AAGACAAACC ATAGAGCAGA ATCTCAGCTC 
ATTGTGGACA ATGCTACAAG AATCTCAAGA CGCCAATTTC CAAAATTTGA TGCAGAACTA 
AATATCTCTG ATAATCCAAC ATTACCCATC TCAATTCAAG ATAAGACATT TATTTTTGTC 
AGGCTCCCAG AAGAGGATGC CAGTTCTCAG TCATCTAAAA CTAATCATCA CTCTTGTTTA 
TATTCTGCAG TTAAAACTTT ACTACAAGAA AATAACTTAC AAAATGCAAA AACATCACAA 
TTTATTGCAT TTAGAAGGCA AGACTGTGAT CTAATTAATG ACTGCTGCTG CAAACACTAC 
ACAGGCCACC TCACCAAAGA CCATCAGAGT AGACTTGTTT TTGGAATTGG TGATAAAATT 
TGTTGTACCA GGAATGCATA CCTCTCAGAC TTACTACCTG AAAATATCTC TGGAAGTCAG 
CAAAATAATG ATCTAGATGC CAGTAGTGAA GACTTTTCTG GTACGCTTCC TGATTTTGCT 
AAAAATAAGC GTGACTTTGA AAGTAACGTT CGACTGTGCA ATGGAGAGAT ATTTTTCATA 
ACAAATGATG TAACTGATGT AACTTTTGGA AAGAGAAGAT CTTTGACCAT TAATAATATG 
GCTGGCCTGG AAGTAACTGT GGATTTTAAG AAACTAATGA AATATTGTCG CATAAAACAT 
GCATGGGCAA GAACTATTCA CACTTTTCAG GGGTCCGAGG AGCAAACAGT TGTCTATGTG 
GTGGGGAAGG CGGGCCGCCA GCACTGGCAG CATGTCTACA CCGCCGTGAC CAGGGGCCGC 
TGCCGAGTGT ATGTGATTGC AGAGGAGTCT CAGCTCCGGA ATGCCATTAT GAAAAACAGT 
TTTCCTAGAA AAACTCGTTT GAAACATTTC TTGCAAAGTA AGCTCTCCTC TAGCGGCGCA 
CCTCCAGCAG ATTTTCCGTC CCCACGGAAG AGCTCTGGAG ACAGTGGAGG ACCCAGCACA 
CCGTCAGCAT CTCCACTCCC TGTAGTCACA GACCACGCCA TGACAAATGA TGTCACCTGG 
AGCGAGGCCT CTTCGCCTGA TGAGAGGACA CTCACCTTTG CTGAAAGATG GCAATTATCT 
TCACCTGATG GAGTAGATAC AGATGATGAT TTACCAAAAT CGCGAGCATC CAAAAGAACC 
TGTGGTGTGA ATGATGATGA AAGTCCAAGC AAAATTTTTA TGGTGGGAGA ATCTCCACAA 
GTGTCTTCCA GACTTCAGAA TTTGAGACTG AATAATTTAA TTCCCAGGCA ACTTTTCAAG 
CCCACCGATA ATCAAGAAAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HELB ( XM_005269234.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005269291.1 Homo sapiens DNA helicase B (HELB), transcript variant X1, mRNA.

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CloneID	OHu46986
Clone ID Related Accession (Same CDS sequence)	XM_005269234.2 , NM_033647.4 , NM_001370285.1
Accession Version	XM_005269234.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	DNA helicase B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005269234.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGGT CGAGTCCGTA CCTGCGCCAA CTTCAGGGAC CTCTGCTCCC ACCCAGGGAT 
CTGGTGGAGG AGGACGACGA CTACCTAAAC GACGACGTGG AGGAGGATGA AGAGTCCGTG 
TTCATCGACG CCGAGGAGCT CTGCAGTGGG GGCGTAAAGG CTGGCAGCCT CCCCGGGTGC 
CTCCGCGTTT CTATTTGTGA TGAAAACACA CAAGAGACAT GTAAAGTGTT TGGACGTTTT 
CCGATAACAG GTGCTTGGTG GAGAGTGAAG GTACAAGTAA AGCCTGTGGT GGGATCAAGG 
AGCTATCAAT ATCAAGTTCA AGGATTTCCG TCTTACTTTT TGCAGTCTGA TATGTCACCA 
CCAAATCAAA AACATATCTG TGCTCTCTTT CTTAAAGAGT GTGAGGTCTC CAGTGATGAT 
GTTAATAAAT TTTTAACATG GGTAAAGGAG GTATCAAACT ACAAAAACCT AAACTTTGAA 
AATCTTAGGG AAACACTAAG AACTTTCCAC AAGGAAACTG GAAGGAAAGA TCAAAAGCAG 
CCTACACAGA ATGGTCAGGA AGAGTTGTTC CTAGACAATG AGATGAGTCT TCCTCTGGAA 
AACACAATTC CATTTAGAAA TGTAATGACA GCTTTGCAGT TTCCGAAGAT AATGGAATTC 
CTTCCAGTTC TTCTGCCTCG ACACTTTAAA TGGATCATAG GGTCAGGTTC TAAAGAGATG 
TTGAAAGAGA TAGAAGAGAT TTTAGGTACA CATCCGTGGA AACTTGGATT TAGTAAAATA 
ACCTACAGAG AGTGGAAACT CCTGCGATGT GAGGCAAGTT GGATAGCATT TTGTCAGTGT 
GAGTCTCTTC TCCAGCTGAT GACTGATTTG GAGAAGAATG CATTAATAAT GTATTCCAGA 
CTGAAGCAGA TATGTAGAGA AGATGGGCAC ACATATGTTG AAGTGAATGA CTTAACTTTG 
ACATTGTCAA ATCATATGTC ATTTCATGCT GCTTCAGAGT CTCTGAAGTT TTTGAAGGAT 
ATTGGTGTGG TGACATATGA GAAGTCCTGT GTCTTCCCTT ATGACCTTTA CCATGCTGAA 
AGAGCCATCG CCTTTTCAAT TTGTGACCTG ATGAAGAAAC CTCCTTGGCA TTTATGTGTC 
GATGTCGAAA AGGTGCTTGC CTCTATTCAC ACCACAAAAC CTGAGAATTC AAGCGATGAT 
GCATTGAATG AGAGCAAACC TGATGAAGTA AGATTAGAAA ATCCTGTGGA TGTTGTGGAC 
ACACAGGACA ATGGTGACCA TATTTGGACT AATGGTGAAA ATGAAATTAA TGCAGAAATA 
AGTGAAGTTC AGCTGGATCA GGATCAGGTT GAAGTTCCAC TGGATCGGGA TCAGGTGGCT 
GCTTTGGAAA TGATTTGCTC CAATCCTGTG ACAGTCATAA GTGGGAAAGG TGGATGTGGG 
AAGACCACAA TCGTTAGCCG TCTTTTTAAG CATATAGAGC AGTTGGAAGA AAGAGAAGTA 
AAAAAAGCCT GTGAAGATTT TGAACAAGAC CAGAATGCTT CAGAAGAATG GATTACCTTT 
ACTGAGCAAA GTCAACTAGA GGCGGACAAG GCTATAGAAG TTTTGCTCAC AGCACCTACA 
GGGAAAGCAG CTGGCTTACT AAGACAGAAA ACTGGTCTTC ATGCCTACAC ACTGTGTCAG 
GTCAATTATA GCTTCTATTC ATGGACTCAA ACAATGATGA CCACAAACAA ACCATGGAAA 
TTTTCTTCGG TTAGAGTTCT GGTTGTGGAT GAAGGGAGTT TGGTATCTGT AGGAATCTTC 
AAATCGGTCT TAAATTTATT GTGTGAGCAC TCCAAACTTT CTAAGCTTAT TATCCTTGGT 
GACATTAGAC AGTTACCCAG TATTGAACCT GGTAACTTGC TGAAAGATCT TTTTGAGACT 
CTTAAGTCAA GAAATTGTGC TATTGAGCTA AAGACAAACC ATAGAGCAGA ATCTCAGCTC 
ATTGTGGACA ATGCTACAAG AATCTCAAGA CGCCAATTTC CAAAATTTGA TGCAGAACTA 
AATATCTCTG ATAATCCAAC ATTACCCATC TCAATTCAAG ATAAGACATT TATTTTTGTC 
AGGCTCCCAG AAGAGGATGC CAGTTCTCAG TCATCTAAAA CTAATCATCA CTCTTGTTTA 
TATTCTGCAG TTAAAACTTT ACTACAAGAA AATAACTTAC AAAATGCAAA AACATCACAA 
TTTATTGCAT TTAGAAGGCA AGACTGTGAT CTAATTAATG ACTGCTGCTG CAAACACTAC 
ACAGGCCACC TCACCAAAGA CCATCAGAGT AGACTTGTTT TTGGAATTGG TGATAAAATT 
TGTTGTACCA GGAATGCATA CCTCTCAGAC TTACTACCTG AAAATATCTC TGGAAGTCAG 
CAAAATAATG ATCTAGATGC CAGTAGTGAA GACTTTTCTG GTACGCTTCC TGATTTTGCT 
AAAAATAAGC GTGACTTTGA AAGTAACGTT CGACTGTGCA ATGGAGAGAT ATTTTTCATA 
ACAAATGATG TAACTGATGT AACTTTTGGA AAGAGAAGAT CTTTGACCAT TAATAATATG 
GCTGGCCTGG AAGTAACTGT GGATTTTAAG AAACTAATGA AATATTGTCG CATAAAACAT 
GCATGGGCAA GAACTATTCA CACTTTTCAG GGGTCCGAGG AGCAAACAGT TGTCTATGTG 
GTGGGGAAGG CGGGCCGCCA GCACTGGCAG CATGTCTACA CCGCCGTGAC CAGGGGCCGC 
TGCCGAGTGT ATGTGATTGC AGAGGAGTCT CAGCTCCGGA ATGCCATTAT GAAAAACAGT 
TTTCCTAGAA AAACTCGTTT GAAACATTTC TTGCAAAGTA AGCTCTCCTC TAGCGGCGCA 
CCTCCAGCAG ATTTTCCGTC CCCACGGAAG AGCTCTGGAG ACAGTGGAGG ACCCAGCACA 
CCGTCAGCAT CTCCACTCCC TGTAGTCACA GACCACGCCA TGACAAATGA TGTCACCTGG 
AGCGAGGCCT CTTCGCCTGA TGAGAGGACA CTCACCTTTG CTGAAAGATG GCAATTATCT 
TCACCTGATG GAGTAGATAC AGATGATGAT TTACCAAAAT CGCGAGCATC CAAAAGAACC 
TGTGGTGTGA ATGATGATGA AAGTCCAAGC AAAATTTTTA TGGTGGGAGA ATCTCCACAA 
GTGTCTTCCA GACTTCAGAA TTTGAGACTG AATAATTTAA TTCCCAGGCA ACTTTTCAAG 
CCCACCGATA ATCAAGAAAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005269291.1
CDS	38..3301
Translation	MARSSPYLRQLQGPLLPPRDLVEEDDDYLNDDVEEDEESVFIDAEELCSGGVKAGSLPGCLRVSICDENTQETCKVFGRFPITGAWWRVKVQVKPVVGSRSYQYQVQGFPSYFLQSDMSPPNQKHICALFLKECEVSSDDVNKFLTWVKEVSNYKNLNFENLRETLRTFHKETGRKDQKQPTQNGQEELFLDNEMSLPLENTIPFRNVMTALQFPKIMEFLPVLLPRHFKWIIGSGSKEMLKEIEEILGTHPWKLGFSKITYREWKLLRCEASWIAFCQCESLLQLMTDLEKNALIMYSRLKQICREDGHTYVEVNDLTLTLSNHMSFHAASESLKFLKDIGVVTYEKSCVFPYDLYHAERAIAFSICDLMKKPPWHLCVDVEKVLASIHTTKPENSSDDALNESKPDEVRLENPVDVVDTQDNGDHIWTNGENEINAEISEVQLDQDQVEVPLDRDQVAALEMICSNPVTVISGKGGCGKTTIVSRLFKHIEQLEEREVKKACEDFEQDQNASEEWITFTEQSQLEADKAIEVLLTAPTGKAAGLLRQKTGLHAYTLCQVNYSFYSWTQTMMTTNKPWKFSSVRVLVVDEGSLVSVGIFKSVLNLLCEHSKLSKLIILGDIRQLPSIEPGNLLKDLFETLKSRNCAIELKTNHRAESQLIVDNATRISRRQFPKFDAELNISDNPTLPISIQDKTFIFVRLPEEDASSQSSKTNHHSCLYSAVKTLLQENNLQNAKTSQFIAFRRQDCDLINDCCCKHYTGHLTKDHQSRLVFGIGDKICCTRNAYLSDLLPENISGSQQNNDLDASSEDFSGTLPDFAKNKRDFESNVRLCNGEIFFITNDVTDVTFGKRRSLTINNMAGLEVTVDFKKLMKYCRIKHAWARTIHTFQGSEEQTVVYVVGKAGRQHWQHVYTAVTRGRCRVYVIAEESQLRNAIMKNSFPRKTRLKHFLQSKLSSSGAPPADFPSPRKSSGDSGGPSTPSASPLPVVTDHAMTNDVTWSEASSPDERTLTFAERWQLSSPDGVDTDDDLPKSRASKRTCGVNDDESPSKIFMVGESPQVSSRLQNLRLNNLIPRQLFKPTDNQET

Target ORF information:

RefSeq Version	XM_005269234.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA helicase B (HELB), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005269234.2

ORF Insert Sequence:

ATGGCCAGGT CGAGTCCGTA CCTGCGCCAA CTTCAGGGAC CTCTGCTCCC ACCCAGGGAT 
CTGGTGGAGG AGGACGACGA CTACCTAAAC GACGACGTGG AGGAGGATGA AGAGTCCGTG 
TTCATCGACG CCGAGGAGCT CTGCAGTGGG GGCGTAAAGG CTGGCAGCCT CCCCGGGTGC 
CTCCGCGTTT CTATTTGTGA TGAAAACACA CAAGAGACAT GTAAAGTGTT TGGACGTTTT 
CCGATAACAG GTGCTTGGTG GAGAGTGAAG GTACAAGTAA AGCCTGTGGT GGGATCAAGG 
AGCTATCAAT ATCAAGTTCA AGGATTTCCG TCTTACTTTT TGCAGTCTGA TATGTCACCA 
CCAAATCAAA AACATATCTG TGCTCTCTTT CTTAAAGAGT GTGAGGTCTC CAGTGATGAT 
GTTAATAAAT TTTTAACATG GGTAAAGGAG GTATCAAACT ACAAAAACCT AAACTTTGAA 
AATCTTAGGG AAACACTAAG AACTTTCCAC AAGGAAACTG GAAGGAAAGA TCAAAAGCAG 
CCTACACAGA ATGGTCAGGA AGAGTTGTTC CTAGACAATG AGATGAGTCT TCCTCTGGAA 
AACACAATTC CATTTAGAAA TGTAATGACA GCTTTGCAGT TTCCGAAGAT AATGGAATTC 
CTTCCAGTTC TTCTGCCTCG ACACTTTAAA TGGATCATAG GGTCAGGTTC TAAAGAGATG 
TTGAAAGAGA TAGAAGAGAT TTTAGGTACA CATCCGTGGA AACTTGGATT TAGTAAAATA 
ACCTACAGAG AGTGGAAACT CCTGCGATGT GAGGCAAGTT GGATAGCATT TTGTCAGTGT 
GAGTCTCTTC TCCAGCTGAT GACTGATTTG GAGAAGAATG CATTAATAAT GTATTCCAGA 
CTGAAGCAGA TATGTAGAGA AGATGGGCAC ACATATGTTG AAGTGAATGA CTTAACTTTG 
ACATTGTCAA ATCATATGTC ATTTCATGCT GCTTCAGAGT CTCTGAAGTT TTTGAAGGAT 
ATTGGTGTGG TGACATATGA GAAGTCCTGT GTCTTCCCTT ATGACCTTTA CCATGCTGAA 
AGAGCCATCG CCTTTTCAAT TTGTGACCTG ATGAAGAAAC CTCCTTGGCA TTTATGTGTC 
GATGTCGAAA AGGTGCTTGC CTCTATTCAC ACCACAAAAC CTGAGAATTC AAGCGATGAT 
GCATTGAATG AGAGCAAACC TGATGAAGTA AGATTAGAAA ATCCTGTGGA TGTTGTGGAC 
ACACAGGACA ATGGTGACCA TATTTGGACT AATGGTGAAA ATGAAATTAA TGCAGAAATA 
AGTGAAGTTC AGCTGGATCA GGATCAGGTT GAAGTTCCAC TGGATCGGGA TCAGGTGGCT 
GCTTTGGAAA TGATTTGCTC CAATCCTGTG ACAGTCATAA GTGGGAAAGG TGGATGTGGG 
AAGACCACAA TCGTTAGCCG TCTTTTTAAG CATATAGAGC AGTTGGAAGA AAGAGAAGTA 
AAAAAAGCCT GTGAAGATTT TGAACAAGAC CAGAATGCTT CAGAAGAATG GATTACCTTT 
ACTGAGCAAA GTCAACTAGA GGCGGACAAG GCTATAGAAG TTTTGCTCAC AGCACCTACA 
GGGAAAGCAG CTGGCTTACT AAGACAGAAA ACTGGTCTTC ATGCCTACAC ACTGTGTCAG 
GTCAATTATA GCTTCTATTC ATGGACTCAA ACAATGATGA CCACAAACAA ACCATGGAAA 
TTTTCTTCGG TTAGAGTTCT GGTTGTGGAT GAAGGGAGTT TGGTATCTGT AGGAATCTTC 
AAATCGGTCT TAAATTTATT GTGTGAGCAC TCCAAACTTT CTAAGCTTAT TATCCTTGGT 
GACATTAGAC AGTTACCCAG TATTGAACCT GGTAACTTGC TGAAAGATCT TTTTGAGACT 
CTTAAGTCAA GAAATTGTGC TATTGAGCTA AAGACAAACC ATAGAGCAGA ATCTCAGCTC 
ATTGTGGACA ATGCTACAAG AATCTCAAGA CGCCAATTTC CAAAATTTGA TGCAGAACTA 
AATATCTCTG ATAATCCAAC ATTACCCATC TCAATTCAAG ATAAGACATT TATTTTTGTC 
AGGCTCCCAG AAGAGGATGC CAGTTCTCAG TCATCTAAAA CTAATCATCA CTCTTGTTTA 
TATTCTGCAG TTAAAACTTT ACTACAAGAA AATAACTTAC AAAATGCAAA AACATCACAA 
TTTATTGCAT TTAGAAGGCA AGACTGTGAT CTAATTAATG ACTGCTGCTG CAAACACTAC 
ACAGGCCACC TCACCAAAGA CCATCAGAGT AGACTTGTTT TTGGAATTGG TGATAAAATT 
TGTTGTACCA GGAATGCATA CCTCTCAGAC TTACTACCTG AAAATATCTC TGGAAGTCAG 
CAAAATAATG ATCTAGATGC CAGTAGTGAA GACTTTTCTG GTACGCTTCC TGATTTTGCT 
AAAAATAAGC GTGACTTTGA AAGTAACGTT CGACTGTGCA ATGGAGAGAT ATTTTTCATA 
ACAAATGATG TAACTGATGT AACTTTTGGA AAGAGAAGAT CTTTGACCAT TAATAATATG 
GCTGGCCTGG AAGTAACTGT GGATTTTAAG AAACTAATGA AATATTGTCG CATAAAACAT 
GCATGGGCAA GAACTATTCA CACTTTTCAG GGGTCCGAGG AGCAAACAGT TGTCTATGTG 
GTGGGGAAGG CGGGCCGCCA GCACTGGCAG CATGTCTACA CCGCCGTGAC CAGGGGCCGC 
TGCCGAGTGT ATGTGATTGC AGAGGAGTCT CAGCTCCGGA ATGCCATTAT GAAAAACAGT 
TTTCCTAGAA AAACTCGTTT GAAACATTTC TTGCAAAGTA AGCTCTCCTC TAGCGGCGCA 
CCTCCAGCAG ATTTTCCGTC CCCACGGAAG AGCTCTGGAG ACAGTGGAGG ACCCAGCACA 
CCGTCAGCAT CTCCACTCCC TGTAGTCACA GACCACGCCA TGACAAATGA TGTCACCTGG 
AGCGAGGCCT CTTCGCCTGA TGAGAGGACA CTCACCTTTG CTGAAAGATG GCAATTATCT 
TCACCTGATG GAGTAGATAC AGATGATGAT TTACCAAAAT CGCGAGCATC CAAAAGAACC 
TGTGGTGTGA ATGATGATGA AAGTCCAAGC AAAATTTTTA TGGTGGGAGA ATCTCCACAA 
GTGTCTTCCA GACTTCAGAA TTTGAGACTG AATAATTTAA TTCCCAGGCA ACTTTTCAAG 
CCCACCGATA ATCAAGAAAC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

HELB Is a Feedback Inhibitor of DNA End Resection.
Molecular cell61(3)405-418(2016 Feb)
Tk?? J,Xu G,Adhikary H,Young JTF,Gallo D,Escribano-D?az C,Krietsch J,Orthwein A,Munro M,Sol W,Al-Hakim A,Lin ZY,Jonkers J,Borst P,Brown GW,Gingras AC,Rottenberg S,Masson JY,Durocher D

Human DNA helicase B interacts with the replication initiation protein Cdc45 and facilitates Cdc45 binding onto chromatin.
Experimental cell research334(2)283-93(2015 Jun)
Gerhardt J,Guler GD,Fanning E

Human DNA helicase B functions in cellular homologous recombination and stimulates Rad51-mediated 5'-3' heteroduplex extension in vitro.
PloS one10(1)e0116852(2015)
Liu H,Yan P,Fanning E

Human DNA helicase B (HDHB) binds to replication protein A and facilitates cellular recovery from replication stress.
The Journal of biological chemistry287(9)6469-81(2012 Feb)
Guler GD,Liu H,Vaithiyalingam S,Arnett DR,Kremmer E,Chazin WJ,Fanning E

Cell cycle-dependent regulation of a human DNA helicase that localizes in DNA damage foci.
Molecular biology of the cell15(7)3320-32(2004 Jul)
Gu J,Xia X,Yan P,Liu H,Podust VN,Reynolds AB,Fanning E

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HELB cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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