HSPB3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HSPB3
Entrez Gene ID	8988
Full Name	heat shock protein family B (small) member 3
Synonyms	DHMN2C,HMN2C,HSPL27
General protein information	Preferred Names heat shock protein family B (small) member 3 Names heat shock protein beta-3 HSP 17 heat shock 17 kDa protein heat shock 27kDa protein 3 protein 3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q11.2
Summary	This gene encodes a muscle-specific small heat shock protein. A mutation in this gene is the cause of autosomal dominant distal hereditary motor neuropathy type 2C.[provided by RefSeq, Sep 2010]. COMPLETENESS: complete on the 3' end.
Disorder MIM:	604624
Disorder Html:	?Neuronopathy, distal hereditary motor, type IIC, 613376 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_006308.2	NP_006299.1	heat shock protein beta-3
NM_006308.3	NP_006299.1	heat shock protein beta-3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	HSPB3	XP_517764.2
Canis lupus familiaris (dog)	HSPB3	XP_005619384.1
Gallus gallus (chicken)	HSPB3	XP_001231558.1
Mus musculus (house mouse)	Hspb3	NP_064344.1
Xenopus tropicalis (tropical clawed frog)	hspb3	XP_002941074.1
Bos taurus (cattle)	HSPB3	NP_001040035.1
Homo sapiens (human)	HSPB3	NP_006299.1
	HSPB3	XP_001096576.1
Rattus norvegicus (Norway rat)	Hspb3	NP_113938.1
Danio rerio (zebrafish)	hspb3	NP_001092922.1

Gene Ontology
Bibliography

Related articles in PubMed

Small Heat Shock Protein B3 (HSPB3) Mutation in an Axonal Charcot-Marie-Tooth Disease Family.
Nam DE, Nam SH, Lee AJ, Hong YB, Choi BO, Chung KW
Journal of the peripheral nervous system : JPNS(2018 Jan)

An interaction study in mammalian cells demonstrates weak binding of HSPB2 to BAG3, which is regulated by HSPB3 and abrogated by HSPB8.
Morelli FF, Mediani L, Heldens L, Bertacchini J, Bigi I, Carr? AD, Vinet J, Carra S
Cell stress & chaperones22(4)531-540(2017 Jul)

HSPB3 protein is expressed in motoneurons and induces their survival after lesion-induced degeneration.
La Padula V, Staszewski O, Nestel S, Busch H, Boerries M, Roussa E, Prinz M, Krieglstein K
Experimental neurology28640-49(2016 Dec)

Structural aspects and chaperone activity of human HspB3: role of the "C-terminal extension".
Asthana A, Raman B, Ramakrishna T, Rao ChM
Cell biochemistry and biophysics64(1)61-72(2012 Sep)

Mutant small heat shock protein B3 causes motor neuropathy: utility of a candidate gene approach.
Kolb SJ, Snyder PJ, Poi EJ, Renard EA, Bartlett A, Gu S, Sutton S, Arnold WD, Freimer ML, Lawson VH, Kissel JT, Prior TW
Neurology74(6)502-6(2010 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study showed that the endogenous HSPB3 protein distribution in the spinal cords of chicken and mouse embryos and in the postnatal nervous system of human.
Title: HSPB3 protein is expressed in motoneurons and induces their survival after lesion-induced degeneration.

HspB3 exhibits chaperone-like activity.
Title: Structural aspects and chaperone activity of human HspB3: role of the "C-terminal extension".

an HSPB3 mutation associated with an axonal motor neuropathy
Title: Mutant small heat shock protein B3 causes motor neuropathy: utility of a candidate gene approach.

Our results suggest that a variety of oligomers composed of different proportions of different sHSPs may form in cell types expressing multiple sHSPs.
Title: Interactions of HSP22 (HSPB8) with HSP20, alphaB-crystallin, and HSPB3.

The following HSPB3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HSPB3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18962	NM_006308.3 Latest version!	Homo sapiens heat shock protein family B (small) member 3 (HSPB3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu18962	NM_006308.2	Homo sapiens heat shock protein family B (small) member 3 (HSPB3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HSPB3 ( NM_006308.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_006299.1 Homo sapiens heat shock protein family B (small) member 3 (HSPB3), mRNA.

Price & Availability↑

CloneID	OHu18962
Clone ID Related Accession (Same CDS sequence)	NM_006308.2 , NM_006308.3
Accession Version	NM_006308.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	heat shock protein beta-3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK314215.1, U15590.1, BX106905.1 and N74672.1. This sequence is a reference standard in the RefSeqGene project. On Sep 15, 2010 this sequence version replaced NM_006308.1. COMPLETENESS: complete on the 3' end.

ATGGCAAAAA TCATTTTGAG GCACCTCATA GAGATTCCAG TGCGTTACCA GGAAGAGTTT 
GAAGCTCGAG GTCTAGAAGA CTGCAGGCTG GATCATGCTT TATATGCACT GCCTGGGCCA 
ACCATCGTGG ACCTGAGGAA AACCAGGGCA GCGCAGTCTC CTCCAGTGGA CTCAGCGGCA 
GAGACGCCAC CCCGAGAAGG CAAATCCCAC TTTCAGATCC TGCTGGACGT GGTCCAGTTC 
CTCCCTGAAG ACATCATCAT TCAGACCTTC GAAGGCTGGC TGCTGATAAA AGCACAACAC 
GGAACCAGAA TGGATGAGCA CGGTTTTATC TCAAGAAGCT TCACCCGACA GTACAAACTA 
CCAGATGGTG TGGAAATCAA AGATTTGTCT GCAGTCCTCT GTCATGATGG AATTTTGGTG 
GTGGAAGTAA AGGATCCAGT TGGGACTAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006299.1
CDS	190..642
Translation	MAKIILRHLIEIPVRYQEEFEARGLEDCRLDHALYALPGPTIVDLRKTRAAQSPPVDSAAETPPREGKSHFQILLDVVQFLPEDIIIQTFEGWLLIKAQHGTRMDEHGFISRSFTRQYKLPDGVEIKDLSAVLCHDGILVVEVKDPVGTK

Target ORF information:

RefSeq Version	NM_006308.2
Organism	Homo sapiens(human)
Definition	Homo sapiens heat shock protein family B (small) member 3 (HSPB3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006308.2

ORF Insert Sequence:

ATGGCAAAAA TCATTTTGAG GCACCTCATA GAGATTCCAG TGCGTTACCA GGAAGAGTTT 
GAAGCTCGAG GTCTAGAAGA CTGCAGGCTG GATCATGCTT TATATGCACT GCCTGGGCCA 
ACCATCGTGG ACCTGAGGAA AACCAGGGCA GCGCAGTCTC CTCCAGTGGA CTCAGCGGCA 
GAGACGCCAC CCCGAGAAGG CAAATCCCAC TTTCAGATCC TGCTGGACGT GGTCCAGTTC 
CTCCCTGAAG ACATCATCAT TCAGACCTTC GAAGGCTGGC TGCTGATAAA AGCACAACAC 
GGAACCAGAA TGGATGAGCA CGGTTTTATC TCAAGAAGCT TCACCCGACA GTACAAACTA 
CCAGATGGTG TGGAAATCAA AGATTTGTCT GCAGTCCTCT GTCATGATGG AATTTTGGTG 
GTGGAAGTAA AGGATCCAGT TGGGACTAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HSPB3 ( NM_006308.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006299.1 Homo sapiens heat shock protein family B (small) member 3 (HSPB3), mRNA.

Price & Availability↑

CloneID	OHu18962
Clone ID Related Accession (Same CDS sequence)	NM_006308.2 , NM_006308.3
Accession Version	NM_006308.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Homo sapiens(human)
Product	heat shock protein beta-3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U15590.1, BX106905.1 and N74672.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_006308.2. ##Evidence-Data-START## Transcript is intronless :: SRR5189655.146331.1, SRR5189655.40478.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000302005.3/ ENSP00000303394.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCAAAAA TCATTTTGAG GCACCTCATA GAGATTCCAG TGCGTTACCA GGAAGAGTTT 
GAAGCTCGAG GTCTAGAAGA CTGCAGGCTG GATCATGCTT TATATGCACT GCCTGGGCCA 
ACCATCGTGG ACCTGAGGAA AACCAGGGCA GCGCAGTCTC CTCCAGTGGA CTCAGCGGCA 
GAGACGCCAC CCCGAGAAGG CAAATCCCAC TTTCAGATCC TGCTGGACGT GGTCCAGTTC 
CTCCCTGAAG ACATCATCAT TCAGACCTTC GAAGGCTGGC TGCTGATAAA AGCACAACAC 
GGAACCAGAA TGGATGAGCA CGGTTTTATC TCAAGAAGCT TCACCCGACA GTACAAACTA 
CCAGATGGTG TGGAAATCAA AGATTTGTCT GCAGTCCTCT GTCATGATGG AATTTTGGTG 
GTGGAAGTAA AGGATCCAGT TGGGACTAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006299.1
CDS	92..544
Translation	MAKIILRHLIEIPVRYQEEFEARGLEDCRLDHALYALPGPTIVDLRKTRAAQSPPVDSAAETPPREGKSHFQILLDVVQFLPEDIIIQTFEGWLLIKAQHGTRMDEHGFISRSFTRQYKLPDGVEIKDLSAVLCHDGILVVEVKDPVGTK

Target ORF information:

RefSeq Version	NM_006308.3
Organism	Homo sapiens(human)
Definition	Homo sapiens heat shock protein family B (small) member 3 (HSPB3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006308.3

ORF Insert Sequence:

ATGGCAAAAA TCATTTTGAG GCACCTCATA GAGATTCCAG TGCGTTACCA GGAAGAGTTT 
GAAGCTCGAG GTCTAGAAGA CTGCAGGCTG GATCATGCTT TATATGCACT GCCTGGGCCA 
ACCATCGTGG ACCTGAGGAA AACCAGGGCA GCGCAGTCTC CTCCAGTGGA CTCAGCGGCA 
GAGACGCCAC CCCGAGAAGG CAAATCCCAC TTTCAGATCC TGCTGGACGT GGTCCAGTTC 
CTCCCTGAAG ACATCATCAT TCAGACCTTC GAAGGCTGGC TGCTGATAAA AGCACAACAC 
GGAACCAGAA TGGATGAGCA CGGTTTTATC TCAAGAAGCT TCACCCGACA GTACAAACTA 
CCAGATGGTG TGGAAATCAA AGATTTGTCT GCAGTCCTCT GTCATGATGG AATTTTGGTG 
GTGGAAGTAA AGGATCCAGT TGGGACTAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Small Heat Shock Protein B3 (HSPB3) Mutation in an Axonal Charcot-Marie-Tooth Disease Family.
Journal of the peripheral nervous system : JPNS(2018 Jan)
Nam DE,Nam SH,Lee AJ,Hong YB,Choi BO,Chung KW

An interaction study in mammalian cells demonstrates weak binding of HSPB2 to BAG3, which is regulated by HSPB3 and abrogated by HSPB8.
Cell stress & chaperones22(4)531-540(2017 Jul)
Morelli FF,Mediani L,Heldens L,Bertacchini J,Bigi I,Carr? AD,Vinet J,Carra S

HSPB3 protein is expressed in motoneurons and induces their survival after lesion-induced degeneration.
Experimental neurology28640-49(2016 Dec)
La Padula V,Staszewski O,Nestel S,Busch H,Boerries M,Roussa E,Prinz M,Krieglstein K

Structural aspects and chaperone activity of human HspB3: role of the "C-terminal extension".
Cell biochemistry and biophysics64(1)61-72(2012 Sep)
Asthana A,Raman B,Ramakrishna T,Rao ChM

Mutant small heat shock protein B3 causes motor neuropathy: utility of a candidate gene approach.
Neurology74(6)502-6(2010 Feb)
Kolb SJ,Snyder PJ,Poi EJ,Renard EA,Bartlett A,Gu S,Sutton S,Arnold WD,Freimer ML,Lawson VH,Kissel JT,Prior TW