HCAR3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HCAR3
Entrez Gene ID	8843
Full Name	hydroxycarboxylic acid receptor 3
Synonyms	GPR109B,HCA3,HM74,PUMAG,Puma-g
General protein information	Preferred Names hydroxycarboxylic acid receptor 3 Names hydroxycarboxylic acid receptor 3 G-protein coupled receptor 109B G-protein coupled receptor HM74 G-protein coupled receptor HM74B GTP-binding protein hydroxy-carboxylic acid receptor 3 niacin receptor 2 nicotinic acid receptor 2 putative chemokine receptor
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q24.31
Disorder MIM:	606039

mRNA and Protein(s)

mRNA	Protein	Name
NM_006018.2	NP_006009.2	hydroxycarboxylic acid receptor 3
NM_006018.3	NP_006009.2	hydroxycarboxylic acid receptor 3

Pathways from BioSystems

Homologies

Homo sapiens (human)	HCAR3	NP_006009.2
Pan troglodytes (chimpanzee)	GPR109B	XP_509519.1
Danio rerio (zebrafish)	hcar1-3	XP_001921237.2
Bos taurus (cattle)	HCAR3	XP_005218034.1
Xenopus tropicalis (tropical clawed frog)	hcar2	XP_004910574.1
Canis lupus familiaris (dog)	HCAR3	XP_005636210.1

Gene Ontology
Bibliography

Related articles in PubMed

The role of the C-terminus of the human hydroxycarboxylic acid receptors 2 and 3 in G protein activation using G?-engineered yeast cells.
Liu R, van Veldhoven JP, IJzerman AP
European journal of pharmacology77070-7(2016 Jan)

Hydroxycarboxylic acid receptors are essential for breast cancer cells to control their lipid/fatty acid metabolism.
St?ubert C, Broom OJ, Nordstr?m A
Oncotarget6(23)19706-20(2015 Aug)

Activated human hydroxy-carboxylic acid receptor-3 signals to MAP kinase cascades via the PLC-dependent PKC and MMP-mediated EGFR pathways.
Zhou Q, Li G, Deng XY, He XB, Chen LJ, Wu C, Shi Y, Wu KP, Mei LJ, Lu JX, Zhou NM
British journal of pharmacology166(6)1756-73(2012 Jul)

International Union of Basic and Clinical Pharmacology. LXXXII: Nomenclature and Classification of Hydroxy-carboxylic Acid Receptors (GPR81, GPR109A, and GPR109B).
Offermanns S, Colletti SL, Lovenberg TW, Semple G, Wise A, IJzerman AP
Pharmacological reviews63(2)269-90(2011 Jun)

Nicotinic acid receptor abnormalities in human skin cancer: implications for a role in epidermal differentiation.
Bermudez Y, Benavente CA, Meyer RG, Coyle WR, Jacobson MK, Jacobson EL
PloS one6(5)e20487(2011)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

new insights into the G protein coupling profiles of the HCA receptors and the function of the receptor's C terminus
Title: The role of the C-terminus of the human hydroxycarboxylic acid receptors 2 and 3 in G protein activation using G?-engineered yeast cells.

HCA1/3 are necessary for breast cancer cells to balance lipid/fatty acid metabolism.
Title: Hydroxycarboxylic acid receptors are essential for breast cancer cells to control their lipid/fatty acid metabolism.

Activated HCAR3 signals to MAP kinase cascades via the PLC-dependent PKC and MMP-mediated EGFR pathways
Title: Activated human hydroxy-carboxylic acid receptor-3 signals to MAP kinase cascades via the PLC-dependent PKC and MMP-mediated EGFR pathways.

In contrast, in a squamous cell carcinoma derived cell line, both GPR109A and GPR109B show a more diffuse cellular localization and the receptors are nearly non-functional.
Title: Nicotinic acid receptor abnormalities in human skin cancer: implications for a role in epidermal differentiation.

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.

The following HCAR3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HCAR3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu30726	NM_006018.3 Latest version!	Homo sapiens hydroxycarboxylic acid receptor 3 (HCAR3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu30726	NM_006018.2	Homo sapiens hydroxycarboxylic acid receptor 3 (HCAR3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HCAR3 ( NM_006018.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_006009.2 Homo sapiens hydroxycarboxylic acid receptor 3 (HCAR3), mRNA.

Price & Availability↑

CloneID	OHu30726
Clone ID Related Accession (Same CDS sequence)	NM_006018.2 , NM_006018.3
Accession Version	NM_006018.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-22
Organism	Homo sapiens(human)
Product	hydroxycarboxylic acid receptor 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from R02740.1, AC026333.28, DA953971.1, BI908162.1, CU451507.1, DA948030.1, BC047891.1, CB529340.1 and CD366840.1. On Sep 28, 2007 this sequence version replaced NM_006018.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. COMPLETENESS: complete on the 3' end.

ATGAATCGGC ACCATCTGCA GGATCACTTT CTGGAAATAG ACAAGAAGAA CTGCTGTGTG 
TTCCGAGATG ACTTCATTGC CAAGGTGTTG CCGCCGGTGT TGGGGCTGGA GTTTATCTTT 
GGGCTTCTGG GCAATGGCCT TGCCCTGTGG ATTTTCTGTT TCCACCTCAA GTCCTGGAAA 
TCCAGCCGGA TTTTCCTGTT CAACCTGGCA GTAGCTGACT TTCTACTGAT CATCTGCCTG 
CCGTTCGTGA TGGACTACTA TGTGCGGCGT TCAGACTGGA AGTTTGGGGA CATCCCTTGC 
CGGCTGGTGC TCTTCATGTT TGCCATGAAC CGCCAGGGCA GCATCATATT CCTCACGGTG 
GTGGCGGTAG ACAGGTATTT CCGGGTGGTC CATCCCCACC ACGCCCTGAA CAAGATCTCC 
AATTGGACAG CAGCCATCAT CTCTTGCCTT CTGTGGGGCA TCACTGTTGG CCTAACAGTC 
CACCTCCTGA AGAAGAAGTT GCTGATCCAG AATGGCACTG CAAATGTGTG CATCAGCTTC 
AGCATCTGCC ATACCTTCCG GTGGCACGAA GCTATGTTCC TCCTGGAGTT CTTCCTGCCC 
CTGGGCATCA TCCTGTTCTG CTCAGCCAGA ATTATCTGGA GCCTGCGGCA GAGACAAATG 
GACCGGCATG CCAAGATCAA GAGAGCCATC ACCTTCATCA TGGTGGTGGC CATCGTCTTT 
GTCATCTGCT TCCTTCCCAG CGTGGTTGTG CGGATCCACA TCTTCTGGCT CCTGCACACT 
TCGGGCACGC AGAATTGTGA AGTGTACCGC TCGGTGGACC TGGCGTTCTT TATCACTCTC 
AGCTTCACCT ACATGAACAG CATGCTGGAC CCCGTGGTGT ACTACTTCTC CAGCCCATCC 
TTTCCCAACT TCTTCTCCAC TTTGATCAAC CGCTGCCTCC AGAGGAAGAT AACAGGTGAG 
CCAGATAATA ACCGCAGCAC GAGCGTCGAG CTCACAGGGG ACCCCAACAA AACCAGAGGC 
GCTCCAGAGG CGTTAATCGC CAACTCCGGT GAGCCATGGA GCCCCTCTTA TCTGGGCCCA 
ACCTCAAATA ACCATTCCAA GAAGGGACAT TGTCACCAAG AACCAGCATC TCTGGAGAAA 
CAGTTGGGCT GTTGCATCGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006009.2
CDS	156..1319
Translation	MNRHHLQDHFLEIDKKNCCVFRDDFIAKVLPPVLGLEFIFGLLGNGLALWIFCFHLKSWKSSRIFLFNLAVADFLLIICLPFVMDYYVRRSDWKFGDIPCRLVLFMFAMNRQGSIIFLTVVAVDRYFRVVHPHHALNKISNWTAAIISCLLWGITVGLTVHLLKKKLLIQNGTANVCISFSICHTFRWHEAMFLLEFFLPLGIILFCSARIIWSLRQRQMDRHAKIKRAITFIMVVAIVFVICFLPSVVVRIHIFWLLHTSGTQNCEVYRSVDLAFFITLSFTYMNSMLDPVVYYFSSPSFPNFFSTLINRCLQRKITGEPDNNRSTSVELTGDPNKTRGAPEALIANSGEPWSPSYLGPTSNNHSKKGHCHQEPASLEKQLGCCIE

Target ORF information:

RefSeq Version	NM_006018.2
Organism	Homo sapiens(human)
Definition	Homo sapiens hydroxycarboxylic acid receptor 3 (HCAR3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006018.2

ORF Insert Sequence:

ATGAATCGGC ACCATCTGCA GGATCACTTT CTGGAAATAG ACAAGAAGAA CTGCTGTGTG 
TTCCGAGATG ACTTCATTGC CAAGGTGTTG CCGCCGGTGT TGGGGCTGGA GTTTATCTTT 
GGGCTTCTGG GCAATGGCCT TGCCCTGTGG ATTTTCTGTT TCCACCTCAA GTCCTGGAAA 
TCCAGCCGGA TTTTCCTGTT CAACCTGGCA GTAGCTGACT TTCTACTGAT CATCTGCCTG 
CCGTTCGTGA TGGACTACTA TGTGCGGCGT TCAGACTGGA AGTTTGGGGA CATCCCTTGC 
CGGCTGGTGC TCTTCATGTT TGCCATGAAC CGCCAGGGCA GCATCATATT CCTCACGGTG 
GTGGCGGTAG ACAGGTATTT CCGGGTGGTC CATCCCCACC ACGCCCTGAA CAAGATCTCC 
AATTGGACAG CAGCCATCAT CTCTTGCCTT CTGTGGGGCA TCACTGTTGG CCTAACAGTC 
CACCTCCTGA AGAAGAAGTT GCTGATCCAG AATGGCACTG CAAATGTGTG CATCAGCTTC 
AGCATCTGCC ATACCTTCCG GTGGCACGAA GCTATGTTCC TCCTGGAGTT CTTCCTGCCC 
CTGGGCATCA TCCTGTTCTG CTCAGCCAGA ATTATCTGGA GCCTGCGGCA GAGACAAATG 
GACCGGCATG CCAAGATCAA GAGAGCCATC ACCTTCATCA TGGTGGTGGC CATCGTCTTT 
GTCATCTGCT TCCTTCCCAG CGTGGTTGTG CGGATCCACA TCTTCTGGCT CCTGCACACT 
TCGGGCACGC AGAATTGTGA AGTGTACCGC TCGGTGGACC TGGCGTTCTT TATCACTCTC 
AGCTTCACCT ACATGAACAG CATGCTGGAC CCCGTGGTGT ACTACTTCTC CAGCCCATCC 
TTTCCCAACT TCTTCTCCAC TTTGATCAAC CGCTGCCTCC AGAGGAAGAT AACAGGTGAG 
CCAGATAATA ACCGCAGCAC GAGCGTCGAG CTCACAGGGG ACCCCAACAA AACCAGAGGC 
GCTCCAGAGG CGTTAATCGC CAACTCCGGT GAGCCATGGA GCCCCTCTTA TCTGGGCCCA 
ACCTCAAATA ACCATTCCAA GAAGGGACAT TGTCACCAAG AACCAGCATC TCTGGAGAAA 
CAGTTGGGCT GTTGCATCGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HCAR3 ( NM_006018.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006009.2 Homo sapiens hydroxycarboxylic acid receptor 3 (HCAR3), mRNA.

Price & Availability↑

CloneID	OHu30726
Clone ID Related Accession (Same CDS sequence)	NM_006018.2 , NM_006018.3
Accession Version	NM_006018.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1164bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Homo sapiens(human)
Product	hydroxycarboxylic acid receptor 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA953971.1, BI908162.1, CU451507.1, DA948030.1, BC047891.1, CB529340.1 and CD366840.1. On May 17, 2019 this sequence version replaced NM_006018.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: BC047891.1, D10923.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000528880.3/ ENSP00000436714.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAATCGGC ACCATCTGCA GGATCACTTT CTGGAAATAG ACAAGAAGAA CTGCTGTGTG 
TTCCGAGATG ACTTCATTGC CAAGGTGTTG CCGCCGGTGT TGGGGCTGGA GTTTATCTTT 
GGGCTTCTGG GCAATGGCCT TGCCCTGTGG ATTTTCTGTT TCCACCTCAA GTCCTGGAAA 
TCCAGCCGGA TTTTCCTGTT CAACCTGGCA GTAGCTGACT TTCTACTGAT CATCTGCCTG 
CCGTTCGTGA TGGACTACTA TGTGCGGCGT TCAGACTGGA AGTTTGGGGA CATCCCTTGC 
CGGCTGGTGC TCTTCATGTT TGCCATGAAC CGCCAGGGCA GCATCATATT CCTCACGGTG 
GTGGCGGTAG ACAGGTATTT CCGGGTGGTC CATCCCCACC ACGCCCTGAA CAAGATCTCC 
AATTGGACAG CAGCCATCAT CTCTTGCCTT CTGTGGGGCA TCACTGTTGG CCTAACAGTC 
CACCTCCTGA AGAAGAAGTT GCTGATCCAG AATGGCACTG CAAATGTGTG CATCAGCTTC 
AGCATCTGCC ATACCTTCCG GTGGCACGAA GCTATGTTCC TCCTGGAGTT CTTCCTGCCC 
CTGGGCATCA TCCTGTTCTG CTCAGCCAGA ATTATCTGGA GCCTGCGGCA GAGACAAATG 
GACCGGCATG CCAAGATCAA GAGAGCCATC ACCTTCATCA TGGTGGTGGC CATCGTCTTT 
GTCATCTGCT TCCTTCCCAG CGTGGTTGTG CGGATCCACA TCTTCTGGCT CCTGCACACT 
TCGGGCACGC AGAATTGTGA AGTGTACCGC TCGGTGGACC TGGCGTTCTT TATCACTCTC 
AGCTTCACCT ACATGAACAG CATGCTGGAC CCCGTGGTGT ACTACTTCTC CAGCCCATCC 
TTTCCCAACT TCTTCTCCAC TTTGATCAAC CGCTGCCTCC AGAGGAAGAT AACAGGTGAG 
CCAGATAATA ACCGCAGCAC GAGCGTCGAG CTCACAGGGG ACCCCAACAA AACCAGAGGC 
GCTCCAGAGG CGTTAATCGC CAACTCCGGT GAGCCATGGA GCCCCTCTTA TCTGGGCCCA 
ACCTCAAATA ACCATTCCAA GAAGGGACAT TGTCACCAAG AACCAGCATC TCTGGAGAAA 
CAGTTGGGCT GTTGCATCGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006009.2
CDS	75..1238
Translation	MNRHHLQDHFLEIDKKNCCVFRDDFIAKVLPPVLGLEFIFGLLGNGLALWIFCFHLKSWKSSRIFLFNLAVADFLLIICLPFVMDYYVRRSDWKFGDIPCRLVLFMFAMNRQGSIIFLTVVAVDRYFRVVHPHHALNKISNWTAAIISCLLWGITVGLTVHLLKKKLLIQNGTANVCISFSICHTFRWHEAMFLLEFFLPLGIILFCSARIIWSLRQRQMDRHAKIKRAITFIMVVAIVFVICFLPSVVVRIHIFWLLHTSGTQNCEVYRSVDLAFFITLSFTYMNSMLDPVVYYFSSPSFPNFFSTLINRCLQRKITGEPDNNRSTSVELTGDPNKTRGAPEALIANSGEPWSPSYLGPTSNNHSKKGHCHQEPASLEKQLGCCIE

Target ORF information:

RefSeq Version	NM_006018.3
Organism	Homo sapiens(human)
Definition	Homo sapiens hydroxycarboxylic acid receptor 3 (HCAR3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006018.3

ORF Insert Sequence:

ATGAATCGGC ACCATCTGCA GGATCACTTT CTGGAAATAG ACAAGAAGAA CTGCTGTGTG 
TTCCGAGATG ACTTCATTGC CAAGGTGTTG CCGCCGGTGT TGGGGCTGGA GTTTATCTTT 
GGGCTTCTGG GCAATGGCCT TGCCCTGTGG ATTTTCTGTT TCCACCTCAA GTCCTGGAAA 
TCCAGCCGGA TTTTCCTGTT CAACCTGGCA GTAGCTGACT TTCTACTGAT CATCTGCCTG 
CCGTTCGTGA TGGACTACTA TGTGCGGCGT TCAGACTGGA AGTTTGGGGA CATCCCTTGC 
CGGCTGGTGC TCTTCATGTT TGCCATGAAC CGCCAGGGCA GCATCATATT CCTCACGGTG 
GTGGCGGTAG ACAGGTATTT CCGGGTGGTC CATCCCCACC ACGCCCTGAA CAAGATCTCC 
AATTGGACAG CAGCCATCAT CTCTTGCCTT CTGTGGGGCA TCACTGTTGG CCTAACAGTC 
CACCTCCTGA AGAAGAAGTT GCTGATCCAG AATGGCACTG CAAATGTGTG CATCAGCTTC 
AGCATCTGCC ATACCTTCCG GTGGCACGAA GCTATGTTCC TCCTGGAGTT CTTCCTGCCC 
CTGGGCATCA TCCTGTTCTG CTCAGCCAGA ATTATCTGGA GCCTGCGGCA GAGACAAATG 
GACCGGCATG CCAAGATCAA GAGAGCCATC ACCTTCATCA TGGTGGTGGC CATCGTCTTT 
GTCATCTGCT TCCTTCCCAG CGTGGTTGTG CGGATCCACA TCTTCTGGCT CCTGCACACT 
TCGGGCACGC AGAATTGTGA AGTGTACCGC TCGGTGGACC TGGCGTTCTT TATCACTCTC 
AGCTTCACCT ACATGAACAG CATGCTGGAC CCCGTGGTGT ACTACTTCTC CAGCCCATCC 
TTTCCCAACT TCTTCTCCAC TTTGATCAAC CGCTGCCTCC AGAGGAAGAT AACAGGTGAG 
CCAGATAATA ACCGCAGCAC GAGCGTCGAG CTCACAGGGG ACCCCAACAA AACCAGAGGC 
GCTCCAGAGG CGTTAATCGC CAACTCCGGT GAGCCATGGA GCCCCTCTTA TCTGGGCCCA 
ACCTCAAATA ACCATTCCAA GAAGGGACAT TGTCACCAAG AACCAGCATC TCTGGAGAAA 
CAGTTGGGCT GTTGCATCGA GTAA