TMEFF1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TMEFF1
Entrez Gene ID	8577
Full Name	transmembrane protein with EGF like and two follistatin like domains 1
Synonyms	C9orf2,CT120.1,H7365,TR-1
General protein information	Preferred Names transmembrane protein with EGF like and two follistatin like domains 1 Names tomoregulin-1 cancer/testis antigen family 120, member 1 transmembrane protein with EGF-like and one follistatin-like domain
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9q31.1
Disorder MIM:	603421

mRNA and Protein(s)

mRNA	Protein	Name
NM_003692.5	NP_003683.2	tomoregulin-1 precursor
NM_003692.4	NP_003683.2	tomoregulin-1 precursor

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	tmeff1	NP_001093738.2
Rattus norvegicus (Norway rat)	Tmeff1	NP_075409.1
Homo sapiens (human)	TMEFF1	NP_003683.2
Mus musculus (house mouse)	Tmeff1	NP_067411.1
Gallus gallus (chicken)	TMEFF1	XP_419072.4
Danio rerio (zebrafish)	tmeff1b	NP_001020472.1
Bos taurus (cattle)	TMEFF1	NP_001192405.1

Gene Ontology
Bibliography

Related articles in PubMed

Follow-up association studies of chromosome region 9q and nonsyndromic cleft lip/palate.
Letra A, Menezes R, Govil M, Fonseca RF, McHenry T, Granjeiro JM, Castilla EE, Orioli IM, Marazita ML, Vieira AR
American journal of medical genetics. Part A152A(7)1701-10(2010 Jul)

Tomoregulin-1 (TMEFF1) inhibits nodal signaling through direct binding to the nodal coreceptor Cripto.
Harms PW, Chang C
Genes & development17(21)2624-9(2003 Nov)

TMEFF1 and brain tumors.
Gery S, Yin D, Xie D, Black KL, Koeffler HP
Oncogene22(18)2723-7(2003 May)

Assignment1 of H7365 (C9orf2) to human chromosome band 9q31 by somatic cell hybrid analysis and fluorescence in situ hybridization.
Eib DW, Merkx GF, Martens GJ, Cremers FP
Cytogenetics and cell genetics81(3-4)180-1(1998)

A novel transmembrane protein with epidermal growth factor and follistatin domains expressed in the hypothalamo-hypophysial axis of Xenopus laevis.
Eib DW, Martens GJ
Journal of neurochemistry67(3)1047-55(1996 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association. (HuGE Navigator)
Title: Follow-up association studies of chromosome region 9q and nonsyndromic cleft lip/palate.

In Xenopus, TMEFF1 blocks nodal signaling through binding to the nodal coreceptor Cripto; it does not associate with either nodal or the type I activin receptor-like kinase (ALK) 4 receptor.
Title: Tomoregulin-1 (TMEFF1) inhibits nodal signaling through direct binding to the nodal coreceptor Cripto.

This protein may behave as a tumor suppressor gene in brain cancers.
Title: TMEFF1 and brain tumors.

The following TMEFF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TMEFF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu32285	NM_003692.5 Latest version!	Homo sapiens transmembrane protein with EGF like and two follistatin like domains 1 (TMEFF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
View Old Accession Versions >>
OHu32285	NM_003692.4	Homo sapiens transmembrane protein with EGF like and two follistatin like domains 1 (TMEFF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TMEFF1 ( NM_003692.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_003683.2 Homo sapiens transmembrane protein with EGF like and two follistatin like domains 1 (TMEFF1), mRNA.

Price & Availability↑

CloneID	OHu32285
Clone ID Related Accession (Same CDS sequence)	NM_003692.5 , NM_003692.4
Accession Version	NM_003692.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	tomoregulin-1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL353805.20, DN602026.1, BC035056.1, BC021991.1 and BF436262.1. On Nov 23, 2018 this sequence version replaced NM_003692.4. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.58274.1, BC035056.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000374879.5/ ENSP00000364013.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGCGCCG CAGCCGCTGA GGCGCCGCTC CGGCTGCCTG CCGCGCCTCC GCTCGCCTTC 
TGCTGCTACA CGTCGGTGCT TCTGCTCTTC GCCTTCTCTC TGCCCGGGAG CCGCGCGTCC 
AACCAGCCCC CGGGTGGTGG CGGCGGCAGC GGCGGGGACT GTCCCGGCGG CAAAGGCAAG 
AGCATCAACT GCTCAGAATT AAATGTGAGG GAGTCTGACG TAAGAGTTTG TGATGAGTCA 
TCATGTAAAT ATGGAGGAGT CTGTAAAGAA GATGGAGATG GTTTGAAATG TGCATGCCAA 
TTTCAGTGCC ATACAAATTA TATTCCTGTC TGTGGATCAA ATGGGGACAC TTATCAAAAT 
GAATGCTTTC TCAGAAGGGC TGCTTGTAAG CACCAGAAAG AGATAACAGT AATAGCAAGA 
GGACCATGCT ACTCTGATAA TGGATCTGGA TCTGGAGAAG GAGAAGAGGA AGGGTCAGGG 
GCAGAAGTTC ACAGAAAACA CTCCAAGTGT GGACCCTGCA AATATAAAGC TGAGTGTGAT 
GAAGATGCAG AAAATGTTGG GTGTGTATGT AATATAGATT GCAGTGGATA CAGTTTTAAT 
CCTGTGTGTG CTTCTGATGG GAGTTCCTAT AACAATCCCT GTTTTGTTCG AGAAGCATCT 
TGTATAAAGC AAGAACAAAT TGATATAAGG CATCTTGGTC ATTGCACAGA TACAGATGAC 
ACTAGTTTGT TGGGAAAGAA AGATGATGGA CTACAATATC GACCAGATGT GAAAGATGCT 
AGTGATCAAA GAGAAGATGT TTATATTGGA AACCACATGC CTTGCCCTGA AAACCTCAAT 
GGTTACTGCA TCCATGGAAA ATGTGAATTC ATCTATTCTA CTCAGAAGGC TTCTTGTAGA 
TGTGAATCTG GCTACACTGG ACAGCACTGT GAAAAGACAG ACTTTAGTAT TCTCTATGTA 
GTGCCAAGTA GGCAAAAGCT CACTCATGTT CTTATTGCAG CAATTATTGG AGCTGTACAG 
ATTGCCATCA TAGTAGCAAT TGTAATGTGC ATAACAAGAA AATGCCCCAA AAACAATAGA 
GGACGTCGAC AGAAGCAAAA CCTAGGTCAT TTTACTTCAG ATACGTCATC CAGAATGGTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003683.2
CDS	397..1539
Translation	MGAAAAEAPLRLPAAPPLAFCCYTSVLLLFAFSLPGSRASNQPPGGGGGSGGDCPGGKGKSINCSELNVRESDVRVCDESSCKYGGVCKEDGDGLKCACQFQCHTNYIPVCGSNGDTYQNECFLRRAACKHQKEITVIARGPCYSDNGSGSGEGEEEGSGAEVHRKHSKCGPCKYKAECDEDAENVGCVCNIDCSGYSFNPVCASDGSSYNNPCFVREASCIKQEQIDIRHLGHCTDTDDTSLLGKKDDGLQYRPDVKDASDQREDVYIGNHMPCPENLNGYCIHGKCEFIYSTQKASCRCESGYTGQHCEKTDFSILYVVPSRQKLTHVLIAAIIGAVQIAIIVAIVMCITRKCPKNNRGRRQKQNLGHFTSDTSSRMV

Target ORF information:

RefSeq Version	NM_003692.5
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein with EGF like and two follistatin like domains 1 (TMEFF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003692.5

ORF Insert Sequence:

ATGGGCGCCG CAGCCGCTGA GGCGCCGCTC CGGCTGCCTG CCGCGCCTCC GCTCGCCTTC 
TGCTGCTACA CGTCGGTGCT TCTGCTCTTC GCCTTCTCTC TGCCCGGGAG CCGCGCGTCC 
AACCAGCCCC CGGGTGGTGG CGGCGGCAGC GGCGGGGACT GTCCCGGCGG CAAAGGCAAG 
AGCATCAACT GCTCAGAATT AAATGTGAGG GAGTCTGACG TAAGAGTTTG TGATGAGTCA 
TCATGTAAAT ATGGAGGAGT CTGTAAAGAA GATGGAGATG GTTTGAAATG TGCATGCCAA 
TTTCAGTGCC ATACAAATTA TATTCCTGTC TGTGGATCAA ATGGGGACAC TTATCAAAAT 
GAATGCTTTC TCAGAAGGGC TGCTTGTAAG CACCAGAAAG AGATAACAGT AATAGCAAGA 
GGACCATGCT ACTCTGATAA TGGATCTGGA TCTGGAGAAG GAGAAGAGGA AGGGTCAGGG 
GCAGAAGTTC ACAGAAAACA CTCCAAGTGT GGACCCTGCA AATATAAAGC TGAGTGTGAT 
GAAGATGCAG AAAATGTTGG GTGTGTATGT AATATAGATT GCAGTGGATA CAGTTTTAAT 
CCTGTGTGTG CTTCTGATGG GAGTTCCTAT AACAATCCCT GTTTTGTTCG AGAAGCATCT 
TGTATAAAGC AAGAACAAAT TGATATAAGG CATCTTGGTC ATTGCACAGA TACAGATGAC 
ACTAGTTTGT TGGGAAAGAA AGATGATGGA CTACAATATC GACCAGATGT GAAAGATGCT 
AGTGATCAAA GAGAAGATGT TTATATTGGA AACCACATGC CTTGCCCTGA AAACCTCAAT 
GGTTACTGCA TCCATGGAAA ATGTGAATTC ATCTATTCTA CTCAGAAGGC TTCTTGTAGA 
TGTGAATCTG GCTACACTGG ACAGCACTGT GAAAAGACAG ACTTTAGTAT TCTCTATGTA 
GTGCCAAGTA GGCAAAAGCT CACTCATGTT CTTATTGCAG CAATTATTGG AGCTGTACAG 
ATTGCCATCA TAGTAGCAAT TGTAATGTGC ATAACAAGAA AATGCCCCAA AAACAATAGA 
GGACGTCGAC AGAAGCAAAA CCTAGGTCAT TTTACTTCAG ATACGTCATC CAGAATGGTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEFF1 ( NM_003692.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003683.2 Homo sapiens transmembrane protein with EGF like and two follistatin like domains 1 (TMEFF1), mRNA.

Price & Availability↑

CloneID	OHu32285
Clone ID Related Accession (Same CDS sequence)	NM_003692.5 , NM_003692.4
Accession Version	NM_003692.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	tomoregulin-1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DN602026.1, BC035056.1, BC021991.1 and BF436262.1. On Nov 10, 2010 this sequence version replaced NM_003692.3. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.58274.1, BC035056.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCGCCG CAGCCGCTGA GGCGCCGCTC CGGCTGCCTG CCGCGCCTCC GCTCGCCTTC 
TGCTGCTACA CGTCGGTGCT TCTGCTCTTC GCCTTCTCTC TGCCCGGGAG CCGCGCGTCC 
AACCAGCCCC CGGGTGGTGG CGGCGGCAGC GGCGGGGACT GTCCCGGCGG CAAAGGCAAG 
AGCATCAACT GCTCAGAATT AAATGTGAGG GAGTCTGACG TAAGAGTTTG TGATGAGTCA 
TCATGTAAAT ATGGAGGAGT CTGTAAAGAA GATGGAGATG GTTTGAAATG TGCATGCCAA 
TTTCAGTGCC ATACAAATTA TATTCCTGTC TGTGGATCAA ATGGGGACAC TTATCAAAAT 
GAATGCTTTC TCAGAAGGGC TGCTTGTAAG CACCAGAAAG AGATAACAGT AATAGCAAGA 
GGACCATGCT ACTCTGATAA TGGATCTGGA TCTGGAGAAG GAGAAGAGGA AGGGTCAGGG 
GCAGAAGTTC ACAGAAAACA CTCCAAGTGT GGACCCTGCA AATATAAAGC TGAGTGTGAT 
GAAGATGCAG AAAATGTTGG GTGTGTATGT AATATAGATT GCAGTGGATA CAGTTTTAAT 
CCTGTGTGTG CTTCTGATGG GAGTTCCTAT AACAATCCCT GTTTTGTTCG AGAAGCATCT 
TGTATAAAGC AAGAACAAAT TGATATAAGG CATCTTGGTC ATTGCACAGA TACAGATGAC 
ACTAGTTTGT TGGGAAAGAA AGATGATGGA CTACAATATC GACCAGATGT GAAAGATGCT 
AGTGATCAAA GAGAAGATGT TTATATTGGA AACCACATGC CTTGCCCTGA AAACCTCAAT 
GGTTACTGCA TCCATGGAAA ATGTGAATTC ATCTATTCTA CTCAGAAGGC TTCTTGTAGA 
TGTGAATCTG GCTACACTGG ACAGCACTGT GAAAAGACAG ACTTTAGTAT TCTCTATGTA 
GTGCCAAGTA GGCAAAAGCT CACTCATGTT CTTATTGCAG CAATTATTGG AGCTGTACAG 
ATTGCCATCA TAGTAGCAAT TGTAATGTGC ATAACAAGAA AATGCCCCAA AAACAATAGA 
GGACGTCGAC AGAAGCAAAA CCTAGGTCAT TTTACTTCAG ATACGTCATC CAGAATGGTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003683.2
CDS	308..1450
Translation	MGAAAAEAPLRLPAAPPLAFCCYTSVLLLFAFSLPGSRASNQPPGGGGGSGGDCPGGKGKSINCSELNVRESDVRVCDESSCKYGGVCKEDGDGLKCACQFQCHTNYIPVCGSNGDTYQNECFLRRAACKHQKEITVIARGPCYSDNGSGSGEGEEEGSGAEVHRKHSKCGPCKYKAECDEDAENVGCVCNIDCSGYSFNPVCASDGSSYNNPCFVREASCIKQEQIDIRHLGHCTDTDDTSLLGKKDDGLQYRPDVKDASDQREDVYIGNHMPCPENLNGYCIHGKCEFIYSTQKASCRCESGYTGQHCEKTDFSILYVVPSRQKLTHVLIAAIIGAVQIAIIVAIVMCITRKCPKNNRGRRQKQNLGHFTSDTSSRMV

Target ORF information:

RefSeq Version	NM_003692.4
Organism	Homo sapiens(human)
Definition	Homo sapiens transmembrane protein with EGF like and two follistatin like domains 1 (TMEFF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003692.4

ORF Insert Sequence:

ATGGGCGCCG CAGCCGCTGA GGCGCCGCTC CGGCTGCCTG CCGCGCCTCC GCTCGCCTTC 
TGCTGCTACA CGTCGGTGCT TCTGCTCTTC GCCTTCTCTC TGCCCGGGAG CCGCGCGTCC 
AACCAGCCCC CGGGTGGTGG CGGCGGCAGC GGCGGGGACT GTCCCGGCGG CAAAGGCAAG 
AGCATCAACT GCTCAGAATT AAATGTGAGG GAGTCTGACG TAAGAGTTTG TGATGAGTCA 
TCATGTAAAT ATGGAGGAGT CTGTAAAGAA GATGGAGATG GTTTGAAATG TGCATGCCAA 
TTTCAGTGCC ATACAAATTA TATTCCTGTC TGTGGATCAA ATGGGGACAC TTATCAAAAT 
GAATGCTTTC TCAGAAGGGC TGCTTGTAAG CACCAGAAAG AGATAACAGT AATAGCAAGA 
GGACCATGCT ACTCTGATAA TGGATCTGGA TCTGGAGAAG GAGAAGAGGA AGGGTCAGGG 
GCAGAAGTTC ACAGAAAACA CTCCAAGTGT GGACCCTGCA AATATAAAGC TGAGTGTGAT 
GAAGATGCAG AAAATGTTGG GTGTGTATGT AATATAGATT GCAGTGGATA CAGTTTTAAT 
CCTGTGTGTG CTTCTGATGG GAGTTCCTAT AACAATCCCT GTTTTGTTCG AGAAGCATCT 
TGTATAAAGC AAGAACAAAT TGATATAAGG CATCTTGGTC ATTGCACAGA TACAGATGAC 
ACTAGTTTGT TGGGAAAGAA AGATGATGGA CTACAATATC GACCAGATGT GAAAGATGCT 
AGTGATCAAA GAGAAGATGT TTATATTGGA AACCACATGC CTTGCCCTGA AAACCTCAAT 
GGTTACTGCA TCCATGGAAA ATGTGAATTC ATCTATTCTA CTCAGAAGGC TTCTTGTAGA 
TGTGAATCTG GCTACACTGG ACAGCACTGT GAAAAGACAG ACTTTAGTAT TCTCTATGTA 
GTGCCAAGTA GGCAAAAGCT CACTCATGTT CTTATTGCAG CAATTATTGG AGCTGTACAG 
ATTGCCATCA TAGTAGCAAT TGTAATGTGC ATAACAAGAA AATGCCCCAA AAACAATAGA 
GGACGTCGAC AGAAGCAAAA CCTAGGTCAT TTTACTTCAG ATACGTCATC CAGAATGGTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Follow-up association studies of chromosome region 9q and nonsyndromic cleft lip/palate.
American journal of medical genetics. Part A152A(7)1701-10(2010 Jul)
Letra A,Menezes R,Govil M,Fonseca RF,McHenry T,Granjeiro JM,Castilla EE,Orioli IM,Marazita ML,Vieira AR

Tomoregulin-1 (TMEFF1) inhibits nodal signaling through direct binding to the nodal coreceptor Cripto.
Genes & development17(21)2624-9(2003 Nov)
Harms PW,Chang C

TMEFF1 and brain tumors.
Oncogene22(18)2723-7(2003 May)
Gery S,Yin D,Xie D,Black KL,Koeffler HP

Assignment1 of H7365 (C9orf2) to human chromosome band 9q31 by somatic cell hybrid analysis and fluorescence in situ hybridization.
Cytogenetics and cell genetics81(3-4)180-1(1998)
Eib DW,Merkx GF,Martens GJ,Cremers FP

A novel transmembrane protein with epidermal growth factor and follistatin domains expressed in the hypothalamo-hypophysial axis of Xenopus laevis.
Journal of neurochemistry67(3)1047-55(1996 Sep)
Eib DW,Martens GJ