NHEJ1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NHEJ1
Entrez Gene ID	79840
Full Name	non-homologous end joining factor 1
Synonyms	XLF
General protein information	Preferred Names non-homologous end joining factor 1 Names non-homologous end-joining factor 1 XRCC4-like factor nonhomologous end-joining factor 1 protein cernunnos
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q35
Summary	Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders. [provided by RefSeq, Jul 2008].
Disorder MIM:	611290
Disorder Html:	Severe combined immunodeficiency with microcephaly, growth retardation, and sensitivity to ionizing radiation, 611291 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_024782.2	NP_079058.1	non-homologous end-joining factor 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	NHEJ1	NP_079058.1
Mus musculus (house mouse)	Nhej1	NP_083618.3
Danio rerio (zebrafish)	nhej1	NP_999914.1
Gallus gallus (chicken)	NHEJ1	XP_422052.2
Xenopus tropicalis (tropical clawed frog)	nhej1	XP_002933989.2
Bos taurus (cattle)	NHEJ1	NP_001068861.1
Canis lupus familiaris (dog)	NHEJ1	XP_853192.2
Rattus norvegicus (Norway rat)	Nhej1	NP_001014239.1
Pan troglodytes (chimpanzee)	NHEJ1	XP_001160321.1

Gene Ontology
Bibliography

Related articles in PubMed

Crystal structure of human XLF: a twist in nonhomologous DNA end-joining.
Andres SN, Modesti M, Tsai CJ, Chu G, Junop MS
Molecular cell28(6)1093-101(2007 Dec)

Cernunnos/XLF promotes the ligation of mismatched and noncohesive DNA ends.
Tsai CJ, Kim SA, Chu G
Proceedings of the National Academy of Sciences of the United States of America104(19)7851-6(2007 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TDP1 participation in human non-homologous end joining (NHEJ) is mediated by interaction with XLF, and that TDP1-XLF interactions and subsequent NHEJ events are regulated by phosphorylation of TDP1-S81.
Title: TDP1 is required for efficient non-homologous end joining in human cells.

The role of XLF in NHEJ is summarized.
Title: XLF/Cernunnos: An important but puzzling participant in the nonhomologous end joining DNA repair pathway.

Although PAXX-deficient cells lack c-NHEJ phenotypes, PAXX forms a stable ternary complex with Ku bound to DNA. Thus, PAXX plays an accessory role during c-NHEJ that is largely overlapped by XLF's function.
Title: PAXX Is an Accessory c-NHEJ Factor that Associates with Ku70 and Has Overlapping Functions with XLF.

XLF has an important role during V(D)J recombination.
Title: XLF deficiency results in reduced N-nucleotide addition during V(D)J recombination.

using dual- and quadruple-trap optical tweezers combined with fluorescence microscopy, we show how human XRCC4, XLF and XRCC4-XLF complexes interact with DNA in real time
Title: Sliding sleeves of XRCC4-XLF bridge DNA and connect fragments of broken DNA.

The following NHEJ1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NHEJ1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu23739	NM_024782.2 Latest version!	Homo sapiens non-homologous end joining factor 1 (NHEJ1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NHEJ1 ( NM_024782.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_079058.1 Homo sapiens non-homologous end joining factor 1 (NHEJ1), mRNA.

Price & Availability↑

CloneID	OHu23739
Clone ID Related Accession (Same CDS sequence)	NM_024782.2
Accession Version	NM_024782.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Homo sapiens(human)
Product	non-homologous end-joining factor 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB124934.1 and AJ972687.1. This sequence is a reference standard in the RefSeqGene project. On May 7, 2008 this sequence version replaced NM_024782.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ972687.1, SRR1803613.180484.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##

ATGGAAGAAC TGGAGCAAGG CCTGTTGATG CAGCCATGGG CGTGGCTACA GCTTGCAGAG 
AACTCCCTCT TGGCCAAGGT TTTTATCACC AAGCAGGGCT ATGCCTTGTT GGTTTCAGAT 
CTTCAACAGG TGTGGCATGA ACAGGTGGAC ACTAGTGTGG TCAGCCAGCG AGCCAAGGAG 
CTGAACAAGC GGCTCACTGC TCCTCCTGCA GCTTTCCTCT GTCATTTGGA TAATCTCCTT 
CGCCCATTGT TGAAGGACGC TGCTCACCCT AGCGAAGCTA CCTTCTCCTG TGATTGTGTG 
GCAGATGCAC TGATTCTACG GGTGCGAAGT GAGCTCTCTG GCCTCCCCTT CTATTGGAAT 
TTCCACTGCA TGCTAGCTAG TCCTTCCCTG GTCTCCCAAC ATTTGATTCG TCCTCTGATG 
GGCATGAGTC TGGCATTACA GTGCCAAGTG AGGGAGCTAG CAACGTTACT TCATATGAAA 
GACCTAGAGA TCCAAGACTA CCAGGAGAGT GGGGCTACGC TGATTCGAGA TCGATTGAAG 
ACAGAACCAT TTGAAGAAAA TTCCTTCTTG GAACAATTTA TGATAGAGAA ACTGCCAGAG 
GCATGCAGCA TTGGTGATGG AAAGCCCTTT GTCATGAATC TGCAGGATCT GTATATGGCA 
GTCACCACAC AAGAGGTCCA AGTGGGACAG AAGCATCAAG GCGCTGGAGA TCCTCATACC 
TCAAACAGTG CTTCCCTGCA AGGAATCGAT AGCCAATGTG TAAACCAGCC AGAACAACTG 
GTCTCCTCAG CCCCAACCCT CTCAGCACCT GAGAAAGAGT CCACGGGTAC TTCAGGCCCT 
CTGCAGAGAC CTCAGCTGTC AAAGGTCAAG AGGAAGAAGC CAAGGGGTCT CTTCAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079058.1
CDS	147..1046
Translation	MEELEQGLLMQPWAWLQLAENSLLAKVFITKQGYALLVSDLQQVWHEQVDTSVVSQRAKELNKRLTAPPAAFLCHLDNLLRPLLKDAAHPSEATFSCDCVADALILRVRSELSGLPFYWNFHCMLASPSLVSQHLIRPLMGMSLALQCQVRELATLLHMKDLEIQDYQESGATLIRDRLKTEPFEENSFLEQFMIEKLPEACSIGDGKPFVMNLQDLYMAVTTQEVQVGQKHQGAGDPHTSNSASLQGIDSQCVNQPEQLVSSAPTLSAPEKESTGTSGPLQRPQLSKVKRKKPRGLFS

Target ORF information:

RefSeq Version	NM_024782.2
Organism	Homo sapiens(human)
Definition	Homo sapiens non-homologous end joining factor 1 (NHEJ1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024782.2

ORF Insert Sequence:

ATGGAAGAAC TGGAGCAAGG CCTGTTGATG CAGCCATGGG CGTGGCTACA GCTTGCAGAG 
AACTCCCTCT TGGCCAAGGT TTTTATCACC AAGCAGGGCT ATGCCTTGTT GGTTTCAGAT 
CTTCAACAGG TGTGGCATGA ACAGGTGGAC ACTAGTGTGG TCAGCCAGCG AGCCAAGGAG 
CTGAACAAGC GGCTCACTGC TCCTCCTGCA GCTTTCCTCT GTCATTTGGA TAATCTCCTT 
CGCCCATTGT TGAAGGACGC TGCTCACCCT AGCGAAGCTA CCTTCTCCTG TGATTGTGTG 
GCAGATGCAC TGATTCTACG GGTGCGAAGT GAGCTCTCTG GCCTCCCCTT CTATTGGAAT 
TTCCACTGCA TGCTAGCTAG TCCTTCCCTG GTCTCCCAAC ATTTGATTCG TCCTCTGATG 
GGCATGAGTC TGGCATTACA GTGCCAAGTG AGGGAGCTAG CAACGTTACT TCATATGAAA 
GACCTAGAGA TCCAAGACTA CCAGGAGAGT GGGGCTACGC TGATTCGAGA TCGATTGAAG 
ACAGAACCAT TTGAAGAAAA TTCCTTCTTG GAACAATTTA TGATAGAGAA ACTGCCAGAG 
GCATGCAGCA TTGGTGATGG AAAGCCCTTT GTCATGAATC TGCAGGATCT GTATATGGCA 
GTCACCACAC AAGAGGTCCA AGTGGGACAG AAGCATCAAG GCGCTGGAGA TCCTCATACC 
TCAAACAGTG CTTCCCTGCA AGGAATCGAT AGCCAATGTG TAAACCAGCC AGAACAACTG 
GTCTCCTCAG CCCCAACCCT CTCAGCACCT GAGAAAGAGT CCACGGGTAC TTCAGGCCCT 
CTGCAGAGAC CTCAGCTGTC AAAGGTCAAG AGGAAGAAGC CAAGGGGTCT CTTCAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Crystal structure of human XLF: a twist in nonhomologous DNA end-joining.
Molecular cell28(6)1093-101(2007 Dec)
Andres SN,Modesti M,Tsai CJ,Chu G,Junop MS

Cernunnos/XLF promotes the ligation of mismatched and noncohesive DNA ends.
Proceedings of the National Academy of Sciences of the United States of America104(19)7851-6(2007 May)
Tsai CJ,Kim SA,Chu G

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NHEJ1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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