CACNA1D gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_011534097.2	XP_011532399.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X13
NM_000720.4	NP_000711.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform a
NM_000720.3	NP_000711.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform a
XM_011534100.2	XP_011532402.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X15
NM_001128840.2	NP_001122312.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform b
XM_017007138.1	XP_016862627.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X3
XM_017007140.1	XP_016862629.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X5
XM_017007143.1	XP_016862632.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X8
XM_017007144.1	XP_016862633.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X9
XM_005265448.3	XP_005265505.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X12
XM_011534099.2	XP_011532401.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X14
NM_001128840.3	NP_001122312.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform b
NM_001128839.2	NP_001122311.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform c
XM_017007137.1	XP_016862626.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X1
XM_017007139.1	XP_016862628.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X4
XM_011534096.2	XP_011532398.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X11
XM_011534094.2	XP_011532396.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X2
XM_017007141.1	XP_016862630.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X6
XM_017007142.1	XP_016862631.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X7
XM_017007145.1	XP_016862634.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform X10
NM_001128839.3	NP_001122311.1	voltage-dependent L-type calcium channel subunit alpha-1D isoform c

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu56580	XM_011534099.2 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu94539	XM_017007137.1 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu94540	XM_017007138.1 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu94541	XM_017007139.1 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu94542	XM_017007140.1 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu94543	XM_017007141.1 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu94544	XM_017007142.1 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu94545	XM_017007143.1 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu94546	XM_017007144.1 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu94547	XM_017007145.1 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu10032	NM_001128839.3 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu10146	NM_001128840.3 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu20038	NM_000720.4 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu37463	XM_005265448.3 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu56576	XM_011534094.2 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu56578	XM_011534096.2 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu56579	XM_011534097.2 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu56581	XM_011534100.2 Latest version!	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OHu20038	NM_000720.3	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu10146	NM_001128840.2	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu10032	NM_001128839.2	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
Hide Old Accession Versions >>

CACNA1D ( XM_011534099.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011532401.1 Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OHu56580
Clone ID Related Accession (Same CDS sequence)	XM_011534099.2
Accession Version	XM_011534099.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011534099.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTCCCC TCCTTCACAT AGCCCTTTTG GTATTATTTG TAATCATAAT CTATGCTATT 
ATAGGATTGG AACTTTTTAT TGGAAAAATG CACAAAACAT GTTTTTTTGC TGACTCAGAT 
ATCGTAGCTG AAGAGGACCC AGCTCCATGT GCGTTCTCAG GGAATGGACG CCAGTGTACT 
GCCAATGGCA CGGAATGTAG GAGTGGCTGG GTTGGCCCGA ACGGAGGCAT CACCAACTTT 
GATAACTTTG CCTTTGCCAT GCTTACTGTG TTTCAGTGCA TCACCATGGA GGGCTGGACA 
GATGTGCTCT ACTGGGTAAA TGATGCGATA GGATGGGAAT GGCCATGGGT GTATTTTGTT 
AGTCTGATCA TCCTTGGCTC ATTTTTCGTC CTTAACCTGG TTCTTGGTGT CCTTAGTGGA 
GAATTCTCAA AGGAAAGAGA GAAGGCAAAA GCACGGGGAG ATTTCCAGAA GCTCCGGGAG 
AAGCAGCAGC TGGAGGAGGA TCTAAAGGGC TACTTGGATT GGATCACCCA AGCTGAGGAC 
ATCGATCCGG AGAATGAGGA AGAAGGAGGA GAGGAAGGCA AACGAAATAC TAGCATGCCC 
ACCAGCGAGA CTGAGTCTGT GAACACAGAG AACGTCAGCG GTGAAGGCGA GAACCGAGGC 
TGCTGTGGAA GTCTCTGGTG CTGGTGGAGA CGGAGAGGCG CGGCCAAGGC GGGGCCCTCT 
GGGTGTCGGC GGTGGGGTCA AGCCATCTCA AAATCCAAAC TCAGCCGACG CTGGCGTCGC 
TGGAACCGAT TCAATCGCAG AAGATGTAGG GCCGCCGTGA AGTCTGTCAC GTTTTACTGG 
CTGGTTATCG TCCTGGTGTT TCTGAACACC TTAACCATTT CCTCTGAGCA CTACAATCAG 
CCAGATTGGT TGACACAGAT TCAAGATATT GCCAACAAAG TCCTCTTGGC TCTGTTCACC 
TGCGAGATGC TGGTAAAAAT GTACAGCTTG GGCCTCCAAG CATATTTCGT CTCTCTTTTC 
AACCGGTTTG ATTGCTTCGT GGTGTGTGGT GGAATCACTG AGACGATCTT GGTGGAACTG 
GAAATCATGT CTCCCCTGGG GATCTCTGTG TTTCGGTGTG TGCGCCTCTT AAGAATCTTC 
AAAGTGACCA GGCACTGGAC TTCCCTGAGC AACTTAGTGG CATCCTTATT AAACTCCATG 
AAGTCCATCG CTTCGCTGTT GCTTCTGCTT TTTCTCTTCA TTATCATCTT TTCCTTGCTT 
GGGATGCAGC TGTTTGGCGG CAAGTTTAAT TTTGATGAAA CGCAAACCAA GCGGAGCACC 
TTTGACAATT TCCCTCAAGC ACTTCTCACA GTGTTCCAGA TCCTGACAGG CGAAGACTGG 
AATGCTGTGA TGTACGATGG CATCATGGCT TACGGGGGCC CATCCTCTTC AGGAATGATC 
GTCTGCATCT ACTTCATCAT CCTCTTCATT TGTGGTAACT ATATTCTACT GAATGTCTTC 
TTGGCCATCG CTGTAGACAA TTTGGCTGAT GCTGAAAGTC TGAACACTGC TCAGAAAGAA 
GAAGCGGAAG AAAAGGAGAG GAAAAAGATT GCCAGAAAAG AGAGCCTAGA AAATAAAAAG 
AACAACAAAC CAGAAGTCAA CCAGATAGCC AACAGTGACA ACAAGGTTAC AATTGATGAC 
TATAGAGAAG AGGATGAAGA CAAGGACCCC TATCCGCCTT GCGATGTGCC AGTAGGGGAA 
GAGGAAGAGG AAGAGGAGGA GGATGAACCT GAGGTTCCTG CCGGACCCCG TCCTCGAAGG 
ATCTCGGAGT TGAACATGAA GGAAAAAATT GCCCCCATCC CTGAAGGGAG CGCTTTCTTC 
ATTCTTAGCA AGACCAACCC GATCCGCGTA GGCTGCCACA AGCTCATCAA CCACCACATC 
TTCACCAACC TCATCCTTGT CTTCATCATG CTGAGCAGCG CTGCCCTGGC CGCAGAGGAC 
CCCATCCGCA GCCACTCCTT CCGGAACACG ATACTGGGTT ACTTTGACTA TGCCTTCACA 
GCCATCTTTA CTGTTGAGAT CCTGTTGAAG ATGACAACTT TTGGAGCTTT CCTCCACAAA 
GGGGCCTTCT GCAGGAACTA CTTCAATTTG CTGGATATGC TGGTGGTTGG GGTGTCTCTG 
GTGTCATTTG GGATTCAATC CAGTGCCATC TCCGTTGTGA AGATTCTGAG GGTCTTAAGG 
GTCCTGCGTC CCCTCAGGGC CATCAACAGA GCAAAAGGAC TTAAGCACGT GGTCCAGTGC 
GTCTTCGTGG CCATCCGGAC CATCGGCAAC ATCATGATCG TCACCACCCT CCTGCAGTTC 
ATGTTTGCCT GTATCGGGGT CCAGTTGTTC AAGGGGAAGT TCTATCGCTG TACGGATGAA 
GCCAAAAGTA ACCCTGAAGA ATGCAGGGGA CTTTTCATCC TCTACAAGGA TGGGGATGTT 
GACAGTCCTG TGGTCCGTGA ACGGATCTGG CAAAACAGTG ATTTCAACTT CGACAACGTC 
CTCTCTGCTA TGATGGCGCT CTTCACAGTC TCCACGTTTG AGGGCTGGCC TGCGTTGCTG 
TATAAAGCCA TCGACTCGAA TGGAGAGAAC ATCGGCCCAA TCTACAACCA CCGCGTGGAG 
ATCTCCATCT TCTTCATCAT CTACATCATC ATTGTAGCTT TCTTCATGAT GAACATCTTT 
GTGGGCTTTG TCATCGTTAC ATTTCAGGAA CAAGGAGAAA AAGAGTATAA GAACTGTGAG 
CTGGACAAAA ATCAGCGTCA GTGTGTTGAA TACGCCTTGA AAGCACGTCC CTTGCGGAGA 
TACATCCCCA AAAACCCCTA CCAGTACAAG TTCTGGTACG TGGTGAACTC TTCGCCTTTC 
GAATACATGA TGTTTGTCCT CATCATGCTC AACACACTCT GCTTGGCCAT GCAGCACTAC 
GAGCAGTCCA AGATGTTCAA TGATGCCATG GACATTCTGA ACATGGTCTT CACCGGGGTG 
TTCACCGTCG AGATGGTTTT GAAAGTCATC GCATTTAAGC CTAAGGGGTA TTTTAGTGAC 
GCCTGGAACA CGTTTGACTC CCTCATCGTA ATCGGCAGCA TTATAGACGT GGCCCTCAGC 
GAAGCAGACC ACTATTTCAC TGATGCATGG AACACTTTTG ATGCCTTAAT TGTTGTTGGT 
AGCGTCGTTG ATATTGCTAT AACTGAAGTG AATCCAACTG AAAGTGAAAA TGTCCCTGTC 
CCAACTGCTA CACCTGGGAA CTCTGAAGAG AGCAATAGAA TCTCCATCAC CTTTTTCCGT 
CTTTTCCGAG TGATGCGATT GGTGAAGCTT CTCAGCAGGG GGGAAGGCAT CCGGACATTG 
CTGTGGACTT TTATTAAGTC CTTTCAGGCG CTCCCGTATG TGGCCCTCCT CATAGCCATG 
CTGTTCTTCA TCTATGCGGT CATTGGCATG CAGATGTTTG GGAAAGTTGC CATGAGAGAT 
AACAACCAGA TCAATAGGAA CAATAACTTC CAGACGTTTC CCCAGGCGGT GCTGCTGCTC 
TTCAGGTGTG CAACAGGTGA GGCCTGGCAG GAGATCATGC TGGCCTGTCT CCCAGGGAAG 
CTCTGTGACC CTGAGTCAGA TTACAACCCC GGGGAGGAGT ATACATGTGG GAGCAACTTT 
GCCATTGTCT ATTTCATCAG TTTTTACATG CTCTGTGCAT TTCTGATCAT CAATCTGTTT 
GTGGCTGTCA TCATGGATAA TTTCGACTAT CTGACCCGGG ACTGGTCTAT TTTGGGGCCT 
CACCATTTAG ATGAATTCAA AAGAATATGG TCAGAATATG ACCCTGAGGC AAAGGGAAGG 
ATAAAACACC TTGATGTGGT CACTCTGCTT CGACGCATCC AGCCTCCCCT GGGGTTTGGG 
AAGTTATGTC CACACAGGGT AGCGTGCAAG AGATTAGTTG CCATGAACAT GCCTCTCAAC 
AGTGACGGGA CAGTCATGTT TAATGCAACC CTGTTTGCTT TGGTTCGAAC GGCTCTTAAG 
ATCAAGACCG AAGGGAACCT GGAGCAAGCT AATGAAGAAC TTCGGGCTGT GATAAAGAAA 
ATTTGGAAGA AAACCAGCAT GAAATTACTT GACCAAGTTG TCCCTCCAGC TGGTGATGAT 
GAGGTAACCG TGGGGAAGTT CTATGCCACT TTCCTGATAC AGGACTACTT TAGGAAATTC 
AAGAAACGGA AAGAACAAGG ACTGGTGGGA AAGTACCCTG CGAAGAACAC CACAATTGCC 
CTACAGGCGG GATTAAGGAC ACTGCATGAC ATTGGGCCAG AAATCCGGCG TGCTATATCG 
TGTGATTTGC AAGATGACGA GCCTGAGGAA ACAAAACGAG AAGAAGAAGA TGATGTGTTC 
AAAAGAAATG GTGCCCTGCT TGGAAACCAT GTCAATCATG TTAATAGTGA TAGGAGAGAT 
TCCCTTCAGC AGACCAATAC CACCCACCGT CCCCTGCATG TCCAAAGGCC TTCAATTCCA 
CCTGCAAGTG ATACTGAGAA ACCGCTGTTT CCTCCAGCAG GAAATTCGGT GTGTCATAAC 
CATCATAACC ATAATTCCAT AGGAAAGCAA GTTCCCACCT CAACAAATGC CAATCTCAAT 
AATGCCAATA TGTCCAAAGC TGCCCATGGA AAGCGGCCCA GCATTGGGAA CCTTGAGCAT 
GTGTCTGAAA ATGGGCATCA TTCTTCCCAC AAGCATGACC GGGAGCCTCA GAGAAGGTCC 
AGTGTGAAAA GAACCCGCTA TTATGAAACT TACATTAGGT CCGACTCAGG AGATGAACAG 
CTCCCAACTA TTTGCCGGGA AGACCCAGAG ATACATGGCT ATTTCAGGGA CCCCCACTGC 
TTGGGGGAGC AGGAGTATTT CAGTAGTGAG GAATGCTACG AGGATGACAG CTCGCCCACC 
TGGAGCAGGC AAAACTATGG CTACTACAGC AGATACCCAG GCAGAAACAT CGACTCTGAG 
AGGCCCCGAG GCTACCATCA TCCCCAAGGA TTCTTGGAGG ACGATGACTC GCCCGTTTGC 
TATGATTCAC GGAGATCTCC AAGGAGACGC CTACTACCTC CCACCCCAGC ATCCCACCGG 
AGATCCTCCT TCAACTTTGA GTGCCTGCGC CGGCAGAGCA GCCAGGAAGA GGTCCCGTCG 
TCTCCCATCT TCCCCCATCG CACGGCCCTG CCTCTGCATC TAATGCAGCA ACAGATCATG 
GCAGTTGCCG GCCTAGATTC AAGTAAAGCC CAGAAGTACT CACCGAGTCA CTCGACCCGG 
TCGTGGGCCA CCCCTCCAGC AACCCCTCCC TACCGGGACT GGACACCGTG CTACACCCCC 
CTGATCCAAG TGGAGCAGTC AGAGGCCCTG GACCAGGTGA ACGGCAGCCT GCCGTCCCTG 
CACCGCAGCT CCTGGTACAC AGACGAGCCC GACATCTCCT ACCGGACTTT CACACCAGCC 
AGCCTGACTG TCCCCAGCAG CTTCCGGAAC AAAAACAGCG ACAAGCAGAG GAGTGCGGAC 
AGCTTGGTGG AGGCAGTCCT GATATCCGAA GGCTTGGGAC GCTATGCAAG GGACCCAAAA 
TTTGTGTCAG CAACAAAACA CGAAATCGCT GATGCCTGTG ACCTCACCAT CGACGAGATG 
GAGAGTGCAG CCAGCACCCT GCTTAATGGG AACGTGCGTC CCCGAGCCAA CGGGGATGTG 
GGCCCCCTCT CACACCGGCA GGACTATGAG CTACAGGACT TTGGTCCTGG CTACAGCGAC 
GAAGAGCCAG ACCCTGGGAG GGATGAGGAG GACCTGGCGG ATGAAATGAT ATGCATCACC 
ACCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011532401.1
CDS	378..6206
Translation	MVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGENRGCCGSLWCWWRRRGAAKAGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENIGPIYNHRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPTESENVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEDDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGNLEHVSENGHHSSHKHDREPQRRSSVKRTRYYETYIRSDSGDEQLPTICREDPEIHGYFRDPHCLGEQEYFSSEECYEDDSSPTWSRQNYGYYSRYPGRNIDSERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPASHRRSSFNFECLRRQSSQEEVPSSPIFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVEQSEALDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGNVRPRANGDVGPLSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_011534099.2
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011534099.2

ORF Insert Sequence:

ATGGTTCCCC TCCTTCACAT AGCCCTTTTG GTATTATTTG TAATCATAAT CTATGCTATT 
ATAGGATTGG AACTTTTTAT TGGAAAAATG CACAAAACAT GTTTTTTTGC TGACTCAGAT 
ATCGTAGCTG AAGAGGACCC AGCTCCATGT GCGTTCTCAG GGAATGGACG CCAGTGTACT 
GCCAATGGCA CGGAATGTAG GAGTGGCTGG GTTGGCCCGA ACGGAGGCAT CACCAACTTT 
GATAACTTTG CCTTTGCCAT GCTTACTGTG TTTCAGTGCA TCACCATGGA GGGCTGGACA 
GATGTGCTCT ACTGGGTAAA TGATGCGATA GGATGGGAAT GGCCATGGGT GTATTTTGTT 
AGTCTGATCA TCCTTGGCTC ATTTTTCGTC CTTAACCTGG TTCTTGGTGT CCTTAGTGGA 
GAATTCTCAA AGGAAAGAGA GAAGGCAAAA GCACGGGGAG ATTTCCAGAA GCTCCGGGAG 
AAGCAGCAGC TGGAGGAGGA TCTAAAGGGC TACTTGGATT GGATCACCCA AGCTGAGGAC 
ATCGATCCGG AGAATGAGGA AGAAGGAGGA GAGGAAGGCA AACGAAATAC TAGCATGCCC 
ACCAGCGAGA CTGAGTCTGT GAACACAGAG AACGTCAGCG GTGAAGGCGA GAACCGAGGC 
TGCTGTGGAA GTCTCTGGTG CTGGTGGAGA CGGAGAGGCG CGGCCAAGGC GGGGCCCTCT 
GGGTGTCGGC GGTGGGGTCA AGCCATCTCA AAATCCAAAC TCAGCCGACG CTGGCGTCGC 
TGGAACCGAT TCAATCGCAG AAGATGTAGG GCCGCCGTGA AGTCTGTCAC GTTTTACTGG 
CTGGTTATCG TCCTGGTGTT TCTGAACACC TTAACCATTT CCTCTGAGCA CTACAATCAG 
CCAGATTGGT TGACACAGAT TCAAGATATT GCCAACAAAG TCCTCTTGGC TCTGTTCACC 
TGCGAGATGC TGGTAAAAAT GTACAGCTTG GGCCTCCAAG CATATTTCGT CTCTCTTTTC 
AACCGGTTTG ATTGCTTCGT GGTGTGTGGT GGAATCACTG AGACGATCTT GGTGGAACTG 
GAAATCATGT CTCCCCTGGG GATCTCTGTG TTTCGGTGTG TGCGCCTCTT AAGAATCTTC 
AAAGTGACCA GGCACTGGAC TTCCCTGAGC AACTTAGTGG CATCCTTATT AAACTCCATG 
AAGTCCATCG CTTCGCTGTT GCTTCTGCTT TTTCTCTTCA TTATCATCTT TTCCTTGCTT 
GGGATGCAGC TGTTTGGCGG CAAGTTTAAT TTTGATGAAA CGCAAACCAA GCGGAGCACC 
TTTGACAATT TCCCTCAAGC ACTTCTCACA GTGTTCCAGA TCCTGACAGG CGAAGACTGG 
AATGCTGTGA TGTACGATGG CATCATGGCT TACGGGGGCC CATCCTCTTC AGGAATGATC 
GTCTGCATCT ACTTCATCAT CCTCTTCATT TGTGGTAACT ATATTCTACT GAATGTCTTC 
TTGGCCATCG CTGTAGACAA TTTGGCTGAT GCTGAAAGTC TGAACACTGC TCAGAAAGAA 
GAAGCGGAAG AAAAGGAGAG GAAAAAGATT GCCAGAAAAG AGAGCCTAGA AAATAAAAAG 
AACAACAAAC CAGAAGTCAA CCAGATAGCC AACAGTGACA ACAAGGTTAC AATTGATGAC 
TATAGAGAAG AGGATGAAGA CAAGGACCCC TATCCGCCTT GCGATGTGCC AGTAGGGGAA 
GAGGAAGAGG AAGAGGAGGA GGATGAACCT GAGGTTCCTG CCGGACCCCG TCCTCGAAGG 
ATCTCGGAGT TGAACATGAA GGAAAAAATT GCCCCCATCC CTGAAGGGAG CGCTTTCTTC 
ATTCTTAGCA AGACCAACCC GATCCGCGTA GGCTGCCACA AGCTCATCAA CCACCACATC 
TTCACCAACC TCATCCTTGT CTTCATCATG CTGAGCAGCG CTGCCCTGGC CGCAGAGGAC 
CCCATCCGCA GCCACTCCTT CCGGAACACG ATACTGGGTT ACTTTGACTA TGCCTTCACA 
GCCATCTTTA CTGTTGAGAT CCTGTTGAAG ATGACAACTT TTGGAGCTTT CCTCCACAAA 
GGGGCCTTCT GCAGGAACTA CTTCAATTTG CTGGATATGC TGGTGGTTGG GGTGTCTCTG 
GTGTCATTTG GGATTCAATC CAGTGCCATC TCCGTTGTGA AGATTCTGAG GGTCTTAAGG 
GTCCTGCGTC CCCTCAGGGC CATCAACAGA GCAAAAGGAC TTAAGCACGT GGTCCAGTGC 
GTCTTCGTGG CCATCCGGAC CATCGGCAAC ATCATGATCG TCACCACCCT CCTGCAGTTC 
ATGTTTGCCT GTATCGGGGT CCAGTTGTTC AAGGGGAAGT TCTATCGCTG TACGGATGAA 
GCCAAAAGTA ACCCTGAAGA ATGCAGGGGA CTTTTCATCC TCTACAAGGA TGGGGATGTT 
GACAGTCCTG TGGTCCGTGA ACGGATCTGG CAAAACAGTG ATTTCAACTT CGACAACGTC 
CTCTCTGCTA TGATGGCGCT CTTCACAGTC TCCACGTTTG AGGGCTGGCC TGCGTTGCTG 
TATAAAGCCA TCGACTCGAA TGGAGAGAAC ATCGGCCCAA TCTACAACCA CCGCGTGGAG 
ATCTCCATCT TCTTCATCAT CTACATCATC ATTGTAGCTT TCTTCATGAT GAACATCTTT 
GTGGGCTTTG TCATCGTTAC ATTTCAGGAA CAAGGAGAAA AAGAGTATAA GAACTGTGAG 
CTGGACAAAA ATCAGCGTCA GTGTGTTGAA TACGCCTTGA AAGCACGTCC CTTGCGGAGA 
TACATCCCCA AAAACCCCTA CCAGTACAAG TTCTGGTACG TGGTGAACTC TTCGCCTTTC 
GAATACATGA TGTTTGTCCT CATCATGCTC AACACACTCT GCTTGGCCAT GCAGCACTAC 
GAGCAGTCCA AGATGTTCAA TGATGCCATG GACATTCTGA ACATGGTCTT CACCGGGGTG 
TTCACCGTCG AGATGGTTTT GAAAGTCATC GCATTTAAGC CTAAGGGGTA TTTTAGTGAC 
GCCTGGAACA CGTTTGACTC CCTCATCGTA ATCGGCAGCA TTATAGACGT GGCCCTCAGC 
GAAGCAGACC ACTATTTCAC TGATGCATGG AACACTTTTG ATGCCTTAAT TGTTGTTGGT 
AGCGTCGTTG ATATTGCTAT AACTGAAGTG AATCCAACTG AAAGTGAAAA TGTCCCTGTC 
CCAACTGCTA CACCTGGGAA CTCTGAAGAG AGCAATAGAA TCTCCATCAC CTTTTTCCGT 
CTTTTCCGAG TGATGCGATT GGTGAAGCTT CTCAGCAGGG GGGAAGGCAT CCGGACATTG 
CTGTGGACTT TTATTAAGTC CTTTCAGGCG CTCCCGTATG TGGCCCTCCT CATAGCCATG 
CTGTTCTTCA TCTATGCGGT CATTGGCATG CAGATGTTTG GGAAAGTTGC CATGAGAGAT 
AACAACCAGA TCAATAGGAA CAATAACTTC CAGACGTTTC CCCAGGCGGT GCTGCTGCTC 
TTCAGGTGTG CAACAGGTGA GGCCTGGCAG GAGATCATGC TGGCCTGTCT CCCAGGGAAG 
CTCTGTGACC CTGAGTCAGA TTACAACCCC GGGGAGGAGT ATACATGTGG GAGCAACTTT 
GCCATTGTCT ATTTCATCAG TTTTTACATG CTCTGTGCAT TTCTGATCAT CAATCTGTTT 
GTGGCTGTCA TCATGGATAA TTTCGACTAT CTGACCCGGG ACTGGTCTAT TTTGGGGCCT 
CACCATTTAG ATGAATTCAA AAGAATATGG TCAGAATATG ACCCTGAGGC AAAGGGAAGG 
ATAAAACACC TTGATGTGGT CACTCTGCTT CGACGCATCC AGCCTCCCCT GGGGTTTGGG 
AAGTTATGTC CACACAGGGT AGCGTGCAAG AGATTAGTTG CCATGAACAT GCCTCTCAAC 
AGTGACGGGA CAGTCATGTT TAATGCAACC CTGTTTGCTT TGGTTCGAAC GGCTCTTAAG 
ATCAAGACCG AAGGGAACCT GGAGCAAGCT AATGAAGAAC TTCGGGCTGT GATAAAGAAA 
ATTTGGAAGA AAACCAGCAT GAAATTACTT GACCAAGTTG TCCCTCCAGC TGGTGATGAT 
GAGGTAACCG TGGGGAAGTT CTATGCCACT TTCCTGATAC AGGACTACTT TAGGAAATTC 
AAGAAACGGA AAGAACAAGG ACTGGTGGGA AAGTACCCTG CGAAGAACAC CACAATTGCC 
CTACAGGCGG GATTAAGGAC ACTGCATGAC ATTGGGCCAG AAATCCGGCG TGCTATATCG 
TGTGATTTGC AAGATGACGA GCCTGAGGAA ACAAAACGAG AAGAAGAAGA TGATGTGTTC 
AAAAGAAATG GTGCCCTGCT TGGAAACCAT GTCAATCATG TTAATAGTGA TAGGAGAGAT 
TCCCTTCAGC AGACCAATAC CACCCACCGT CCCCTGCATG TCCAAAGGCC TTCAATTCCA 
CCTGCAAGTG ATACTGAGAA ACCGCTGTTT CCTCCAGCAG GAAATTCGGT GTGTCATAAC 
CATCATAACC ATAATTCCAT AGGAAAGCAA GTTCCCACCT CAACAAATGC CAATCTCAAT 
AATGCCAATA TGTCCAAAGC TGCCCATGGA AAGCGGCCCA GCATTGGGAA CCTTGAGCAT 
GTGTCTGAAA ATGGGCATCA TTCTTCCCAC AAGCATGACC GGGAGCCTCA GAGAAGGTCC 
AGTGTGAAAA GAACCCGCTA TTATGAAACT TACATTAGGT CCGACTCAGG AGATGAACAG 
CTCCCAACTA TTTGCCGGGA AGACCCAGAG ATACATGGCT ATTTCAGGGA CCCCCACTGC 
TTGGGGGAGC AGGAGTATTT CAGTAGTGAG GAATGCTACG AGGATGACAG CTCGCCCACC 
TGGAGCAGGC AAAACTATGG CTACTACAGC AGATACCCAG GCAGAAACAT CGACTCTGAG 
AGGCCCCGAG GCTACCATCA TCCCCAAGGA TTCTTGGAGG ACGATGACTC GCCCGTTTGC 
TATGATTCAC GGAGATCTCC AAGGAGACGC CTACTACCTC CCACCCCAGC ATCCCACCGG 
AGATCCTCCT TCAACTTTGA GTGCCTGCGC CGGCAGAGCA GCCAGGAAGA GGTCCCGTCG 
TCTCCCATCT TCCCCCATCG CACGGCCCTG CCTCTGCATC TAATGCAGCA ACAGATCATG 
GCAGTTGCCG GCCTAGATTC AAGTAAAGCC CAGAAGTACT CACCGAGTCA CTCGACCCGG 
TCGTGGGCCA CCCCTCCAGC AACCCCTCCC TACCGGGACT GGACACCGTG CTACACCCCC 
CTGATCCAAG TGGAGCAGTC AGAGGCCCTG GACCAGGTGA ACGGCAGCCT GCCGTCCCTG 
CACCGCAGCT CCTGGTACAC AGACGAGCCC GACATCTCCT ACCGGACTTT CACACCAGCC 
AGCCTGACTG TCCCCAGCAG CTTCCGGAAC AAAAACAGCG ACAAGCAGAG GAGTGCGGAC 
AGCTTGGTGG AGGCAGTCCT GATATCCGAA GGCTTGGGAC GCTATGCAAG GGACCCAAAA 
TTTGTGTCAG CAACAAAACA CGAAATCGCT GATGCCTGTG ACCTCACCAT CGACGAGATG 
GAGAGTGCAG CCAGCACCCT GCTTAATGGG AACGTGCGTC CCCGAGCCAA CGGGGATGTG 
GGCCCCCTCT CACACCGGCA GGACTATGAG CTACAGGACT TTGGTCCTGG CTACAGCGAC 
GAAGAGCCAG ACCCTGGGAG GGATGAGGAG GACCTGGCGG ATGAAATGAT ATGCATCACC 
ACCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_017007137.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862626.1 Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X1, mRNA.

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CloneID	OHu94539
Clone ID Related Accession (Same CDS sequence)	XM_017007137.1
Accession Version	XM_017007137.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGGG AGCCGCTGCC GCTGTGGGGC TCCCCTCCCC AGCTCTGGCC CGGGCACCAC 
GTGCAGCTTC CACGCCGGCC GGGGCTGGGT CGAGGGAGGA TGGAGCAGCT CCTGGCCAGC 
CCTCCCCTTT GGAGACCGCC AGTGCCCCCC AACCCCTGTC GGCTGGAAGC CTTTCCGGAG 
GCAAACTATG CAAGAGGCAC CAGACTTCCT CTTTCTGGTG AAGGACCAAC TTCTCAGCCG 
AATAGCTCCA AGCAAACTGT CCTGTCTTGG CAAGCTGCAA TCGATGCTGC TAGACAGGCC 
AAGGCTGCCC AAACTATGAG CACCTCTGCA CCCCCACCTG TAGGATCTCT CTCCCAAAGA 
AAACGTCAGC AATACGCCAA GAGCAAAAAA CAGGGTAACT CGTCCAACAG CCGACCTGCC 
CGCGCCCTTT TCTGTTTATC ACTCAATAAC CCCATCCGAA GAGCCTGCAT TAGTATAGTG 
GAATGGAAAC CATTTGACAT ATTTATATTA TTGGCTATTT TTGCCAATTG TGTGGCCTTA 
GCTATTTACA TCCCATTCCC TGAAGATGAT TCTAATTCAA CAAATCATAA CTTGGAAAAA 
GTAGAATATG CCTTCCTGAT TATTTTTACA GTCGAGACAT TTTTGAAGAT TATAGCGTAT 
GGATTATTGC TACATCCTAA TGCTTATGTT AGGAATGGAT GGAATTTACT GGATTTTGTT 
ATAGTAATAG TAGGATTGTT TAGTGTAATT TTGGAACAAT TAACCAAAGA AACAGAAGGC 
GGGAACCACT CAAGCGGCAA ATCTGGAGGC TTTGATGTCA AAGCCCTCCG TGCCTTTCGA 
GTGTTGCGAC CACTTCGACT AGTGTCAGGA GTGCCCAGTT TACAAGTTGT CCTGAACTCC 
ATTATAAAAG CCATGGTTCC CCTCCTTCAC ATAGCCCTTT TGGTATTATT TGTAATCATA 
ATCTATGCTA TTATAGGATT GGAACTTTTT ATTGGAAAAA TGCACAAAAC ATGTTTTTTT 
GCTGACTCAG ATATCGTAGC TGAAGAGGAC CCAGCTCCAT GTGCGTTCTC AGGGAATGGA 
CGCCAGTGTA CTGCCAATGG CACGGAATGT AGGAGTGGCT GGGTTGGCCC GAACGGAGGC 
ATCACCAACT TTGATAACTT TGCCTTTGCC ATGCTTACTG TGTTTCAGTG CATCACCATG 
GAGGGCTGGA CAGATGTGCT CTACTGGGTA AATGATGCGA TAGGATGGGA ATGGCCATGG 
GTGTATTTTG TTAGTCTGAT CATCCTTGGC TCATTTTTCG TCCTTAACCT GGTTCTTGGT 
GTCCTTAGTG GAGAATTCTC AAAGGAAAGA GAGAAGGCAA AAGCACGGGG AGATTTCCAG 
AAGCTCCGGG AGAAGCAGCA GCTGGAGGAG GATCTAAAGG GCTACTTGGA TTGGATCACC 
CAAGCTGAGG ACATCGATCC GGAGAATGAG GAAGAAGGAG GAGAGGAAGG CAAACGAAAT 
ACTAGCATGC CCACCAGCGA GACTGAGTCT GTGAACACAG AGAACGTCAG CGGTGAAGGC 
GAGAACCGAG GCTGCTGTGG AAGTCTCTGG TGCTGGTGGA GACGGAGAGG CGCGGCCAAG 
GCGGGGCCCT CTGGGTGTCG GCGGTGGGGT CAAGCCATCT CAAAATCCAA ACTCAGCCGA 
CGCTGGCGTC GCTGGAACCG ATTCAATCGC AGAAGATGTA GGGCCGCCGT GAAGTCTGTC 
ACGTTTTACT GGCTGGTTAT CGTCCTGGTG TTTCTGAACA CCTTAACCAT TTCCTCTGAG 
CACTACAATC AGCCAGATTG GTTGACACAG ATTCAAGATA TTGCCAACAA AGTCCTCTTG 
GCTCTGTTCA CCTGCGAGAT GCTGGTAAAA ATGTACAGCT TGGGCCTCCA AGCATATTTC 
GTCTCTCTTT TCAACCGGTT TGATTGCTTC GTGGTGTGTG GTGGAATCAC TGAGACGATC 
TTGGTGGAAC TGGAAATCAT GTCTCCCCTG GGGATCTCTG TGTTTCGGTG TGTGCGCCTC 
TTAAGAATCT TCAAAGTGAC CAGGCACTGG ACTTCCCTGA GCAACTTAGT GGCATCCTTA 
TTAAACTCCA TGAAGTCCAT CGCTTCGCTG TTGCTTCTGC TTTTTCTCTT CATTATCATC 
TTTTCCTTGC TTGGGATGCA GCTGTTTGGC GGCAAGTTTA ATTTTGATGA AACGCAAACC 
AAGCGGAGCA CCTTTGACAA TTTCCCTCAA GCACTTCTCA CAGTGTTCCA GATCCTGACA 
GGCGAAGACT GGAATGCTGT GATGTACGAT GGCATCATGG CTTACGGGGG CCCATCCTCT 
TCAGGAATGA TCGTCTGCAT CTACTTCATC ATCCTCTTCA TTTGTGGTAA CTATATTCTA 
CTGAATGTCT TCTTGGCCAT CGCTGTAGAC AATTTGGCTG ATGCTGAAAG TCTGAACACT 
GCTCAGAAAG AAGAAGCGGA AGAAAAGGAG AGGAAAAAGA TTGCCAGAAA AGAGAGCCTA 
GAAAATAAAA AGAACAACAA ACCAGAAGTC AACCAGATAG CCAACAGTGA CAACAAGGTT 
ACAATTGATG ACTATAGAGA AGAGGATGAA GACAAGGACC CCTATCCGCC TTGCGATGTG 
CCAGTAGGGG AAGAGGAAGA GGAAGAGGAG GAGGATGAAC CTGAGGTTCC TGCCGGACCC 
CGTCCTCGAA GGATCTCGGA GTTGAACATG AAGGAAAAAA TTGCCCCCAT CCCTGAAGGG 
AGCGCTTTCT TCATTCTTAG CAAGACCAAC CCGATCCGCG TAGGCTGCCA CAAGCTCATC 
AACCACCACA TCTTCACCAA CCTCATCCTT GTCTTCATCA TGCTGAGCAG CGCTGCCCTG 
GCCGCAGAGG ACCCCATCCG CAGCCACTCC TTCCGGAACA CGATACTGGG TTACTTTGAC 
TATGCCTTCA CAGCCATCTT TACTGTTGAG ATCCTGTTGA AGATGACAAC TTTTGGAGCT 
TTCCTCCACA AAGGGGCCTT CTGCAGGAAC TACTTCAATT TGCTGGATAT GCTGGTGGTT 
GGGGTGTCTC TGGTGTCATT TGGGATTCAA TCCAGTGCCA TCTCCGTTGT GAAGATTCTG 
AGGGTCTTAA GGGTCCTGCG TCCCCTCAGG GCCATCAACA GAGCAAAAGG ACTTAAGCAC 
GTGGTCCAGT GCGTCTTCGT GGCCATCCGG ACCATCGGCA ACATCATGAT CGTCACCACC 
CTCCTGCAGT TCATGTTTGC CTGTATCGGG GTCCAGTTGT TCAAGGGGAA GTTCTATCGC 
TGTACGGATG AAGCCAAAAG TAACCCTGAA GAATGCAGGG GACTTTTCAT CCTCTACAAG 
GATGGGGATG TTGACAGTCC TGTGGTCCGT GAACGGATCT GGCAAAACAG TGATTTCAAC 
TTCGACAACG TCCTCTCTGC TATGATGGCG CTCTTCACAG TCTCCACGTT TGAGGGCTGG 
CCTGCGTTGC TGTATAAAGC CATCGACTCG AATGGAGAGA ACATCGGCCC AATCTACAAC 
CACCGCGTGG AGATCTCCAT CTTCTTCATC ATCTACATCA TCATTGTAGC TTTCTTCATG 
ATGAACATCT TTGTGGGCTT TGTCATCGTT ACATTTCAGG AACAAGGAGA AAAAGAGTAT 
AAGAACTGTG AGCTGGACAA AAATCAGCGT CAGTGTGTTG AATACGCCTT GAAAGCACGT 
CCCTTGCGGA GATACATCCC CAAAAACCCC TACCAGTACA AGTTCTGGTA CGTGGTGAAC 
TCTTCGCCTT TCGAATACAT GATGTTTGTC CTCATCATGC TCAACACACT CTGCTTGGCC 
ATGCAGCACT ACGAGCAGTC CAAGATGTTC AATGATGCCA TGGACATTCT GAACATGGTC 
TTCACCGGGG TGTTCACCGT CGAGATGGTT TTGAAAGTCA TCGCATTTAA GCCTAAGGGG 
TATTTTAGTG ACGCCTGGAA CACGTTTGAC TCCCTCATCG TAATCGGCAG CATTATAGAC 
GTGGCCCTCA GCGAAGCAGA CCACTATTTC ACTGATGCAT GGAACACTTT TGATGCCTTA 
ATTGTTGTTG GTAGCGTCGT TGATATTGCT ATAACTGAAG TGAATCCAAC TGAAAGTGAA 
AATGTCCCTG TCCCAACTGC TACACCTGGG AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGA TTGGTGAAGC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACAT TGCTGTGGAC TTTTATTAAG TCCTTTCAGG CGCTCCCGTA TGTGGCCCTC 
CTCATAGCCA TGCTGTTCTT CATCTATGCG GTCATTGGCA TGCAGATGTT TGGGAAAGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAATAACT TCCAGACGTT TCCCCAGGCG 
GTGCTGCTGC TCTTCAGGTG TGCAACAGGT GAGGCCTGGC AGGAGATCAT GCTGGCCTGT 
CTCCCAGGGA AGCTCTGTGA CCCTGAGTCA GATTACAACC CCGGGGAGGA GTATACATGT 
GGGAGCAACT TTGCCATTGT CTATTTCATC AGTTTTTACA TGCTCTGTGC ATTTCTGATC 
ATCAATCTGT TTGTGGCTGT CATCATGGAT AATTTCGACT ATCTGACCCG GGACTGGTCT 
ATTTTGGGGC CTCACCATTT AGATGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAG 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTCTGC TTCGACGCAT CCAGCCTCCC 
CTGGGGTTTG GGAAGTTATG TCCACACAGG GTAGCGTGCA AGAGATTAGT TGCCATGAAC 
ATGCCTCTCA ACAGTGACGG GACAGTCATG TTTAATGCAA CCCTGTTTGC TTTGGTTCGA 
ACGGCTCTTA AGATCAAGAC CGAAGGGAAC CTGGAGCAAG CTAATGAAGA ACTTCGGGCT 
GTGATAAAGA AAATTTGGAA GAAAACCAGC ATGAAATTAC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAGTACCC TGCGAAGAAC 
ACCACAATTG CCCTACAGGC GGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CGTGTGATTT GCAAGATGAC GAGCCTGAGG AAACAAAACG AGAAGAAGAA 
GATGATGTGT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ATTCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAATTC CACCTGCAAG TGATACTGAG AAACCGCTGT TTCCTCCAGC AGGAAATTCG 
GTGTGTCATA ACCATCATAA CCATAATTCC ATAGGAAAGC AAGTTCCCAC CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTCCAAA GCTGCCCATG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC ATGTGTCTGA AAATGGGCAT CATTCTTCCC ACAAGCATGA CCGGGAGCCT 
CAGAGAAGGT CCAGTGTGAA AAGAACCCGC TATTATGAAA CTTACATTAG GTCCGACTCA 
GGAGATGAAC AGCTCCCAAC TATTTGCCGG GAAGACCCAG AGATACATGG CTATTTCAGG 
GACCCCCACT GCTTGGGGGA GCAGGAGTAT TTCAGTAGTG AGGAATGCTA CGAGGATGAC 
AGCTCGCCCA CCTGGAGCAG GCAAAACTAT GGCTACTACA GCAGATACCC AGGCAGAAAC 
ATCGACTCTG AGAGGCCCCG AGGCTACCAT CATCCCCAAG GATTCTTGGA GGACGATGAC 
TCGCCCGTTT GCTATGATTC ACGGAGATCT CCAAGGAGAC GCCTACTACC TCCCACCCCA 
GCATCCCACC GGAGATCCTC CTTCAACTTT GAGTGCCTGC GCCGGCAGAG CAGCCAGGAA 
GAGGTCCCGT CGTCTCCCAT CTTCCCCCAT CGCACGGCCC TGCCTCTGCA TCTAATGCAG 
CAACAGATCA TGGCAGTTGC CGGCCTAGAT TCAAGTAAAG CCCAGAAGTA CTCACCGAGT 
CACTCGACCC GGTCGTGGGC CACCCCTCCA GCAACCCCTC CCTACCGGGA CTGGACACCG 
TGCTACACCC CCCTGATCCA AGTGGAGCAG TCAGAGGCCC TGGACCAGGT GAACGGCAGC 
CTGCCGTCCC TGCACCGCAG CTCCTGGTAC ACAGACGAGC CCGACATCTC CTACCGGACT 
TTCACACCAG CCAGCCTGAC TGTCCCCAGC AGCTTCCGGA ACAAAAACAG CGACAAGCAG 
AGGAGTGCGG ACAGCTTGGT GGAGGCAGTC CTGATATCCG AAGGCTTGGG ACGCTATGCA 
AGGGACCCAA AATTTGTGTC AGCAACAAAA CACGAAATCG CTGATGCCTG TGACCTCACC 
ATCGACGAGA TGGAGAGTGC AGCCAGCACC CTGCTTAATG GGAACGTGCG TCCCCGAGCC 
AACGGGGATG TGGGCCCCCT CTCACACCGG CAGGACTATG AGCTACAGGA CTTTGGTCCT 
GGCTACAGCG ACGAAGAGCC AGACCCTGGG AGGGATGAGG AGGACCTGGC GGATGAAATG 
ATATGCATCA CCACCTTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862626.1
CDS	16..6756
Translation	MPGEPLPLWGSPPQLWPGHHVQLPRRPGLGRGRMEQLLASPPLWRPPVPPNPCRLEAFPEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGENRGCCGSLWCWWRRRGAAKAGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENIGPIYNHRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPTESENVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEDDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGNLEHVSENGHHSSHKHDREPQRRSSVKRTRYYETYIRSDSGDEQLPTICREDPEIHGYFRDPHCLGEQEYFSSEECYEDDSSPTWSRQNYGYYSRYPGRNIDSERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPASHRRSSFNFECLRRQSSQEEVPSSPIFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVEQSEALDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGNVRPRANGDVGPLSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017007137.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007137.1

ORF Insert Sequence:

ATGCCCGGGG AGCCGCTGCC GCTGTGGGGC TCCCCTCCCC AGCTCTGGCC CGGGCACCAC 
GTGCAGCTTC CACGCCGGCC GGGGCTGGGT CGAGGGAGGA TGGAGCAGCT CCTGGCCAGC 
CCTCCCCTTT GGAGACCGCC AGTGCCCCCC AACCCCTGTC GGCTGGAAGC CTTTCCGGAG 
GCAAACTATG CAAGAGGCAC CAGACTTCCT CTTTCTGGTG AAGGACCAAC TTCTCAGCCG 
AATAGCTCCA AGCAAACTGT CCTGTCTTGG CAAGCTGCAA TCGATGCTGC TAGACAGGCC 
AAGGCTGCCC AAACTATGAG CACCTCTGCA CCCCCACCTG TAGGATCTCT CTCCCAAAGA 
AAACGTCAGC AATACGCCAA GAGCAAAAAA CAGGGTAACT CGTCCAACAG CCGACCTGCC 
CGCGCCCTTT TCTGTTTATC ACTCAATAAC CCCATCCGAA GAGCCTGCAT TAGTATAGTG 
GAATGGAAAC CATTTGACAT ATTTATATTA TTGGCTATTT TTGCCAATTG TGTGGCCTTA 
GCTATTTACA TCCCATTCCC TGAAGATGAT TCTAATTCAA CAAATCATAA CTTGGAAAAA 
GTAGAATATG CCTTCCTGAT TATTTTTACA GTCGAGACAT TTTTGAAGAT TATAGCGTAT 
GGATTATTGC TACATCCTAA TGCTTATGTT AGGAATGGAT GGAATTTACT GGATTTTGTT 
ATAGTAATAG TAGGATTGTT TAGTGTAATT TTGGAACAAT TAACCAAAGA AACAGAAGGC 
GGGAACCACT CAAGCGGCAA ATCTGGAGGC TTTGATGTCA AAGCCCTCCG TGCCTTTCGA 
GTGTTGCGAC CACTTCGACT AGTGTCAGGA GTGCCCAGTT TACAAGTTGT CCTGAACTCC 
ATTATAAAAG CCATGGTTCC CCTCCTTCAC ATAGCCCTTT TGGTATTATT TGTAATCATA 
ATCTATGCTA TTATAGGATT GGAACTTTTT ATTGGAAAAA TGCACAAAAC ATGTTTTTTT 
GCTGACTCAG ATATCGTAGC TGAAGAGGAC CCAGCTCCAT GTGCGTTCTC AGGGAATGGA 
CGCCAGTGTA CTGCCAATGG CACGGAATGT AGGAGTGGCT GGGTTGGCCC GAACGGAGGC 
ATCACCAACT TTGATAACTT TGCCTTTGCC ATGCTTACTG TGTTTCAGTG CATCACCATG 
GAGGGCTGGA CAGATGTGCT CTACTGGGTA AATGATGCGA TAGGATGGGA ATGGCCATGG 
GTGTATTTTG TTAGTCTGAT CATCCTTGGC TCATTTTTCG TCCTTAACCT GGTTCTTGGT 
GTCCTTAGTG GAGAATTCTC AAAGGAAAGA GAGAAGGCAA AAGCACGGGG AGATTTCCAG 
AAGCTCCGGG AGAAGCAGCA GCTGGAGGAG GATCTAAAGG GCTACTTGGA TTGGATCACC 
CAAGCTGAGG ACATCGATCC GGAGAATGAG GAAGAAGGAG GAGAGGAAGG CAAACGAAAT 
ACTAGCATGC CCACCAGCGA GACTGAGTCT GTGAACACAG AGAACGTCAG CGGTGAAGGC 
GAGAACCGAG GCTGCTGTGG AAGTCTCTGG TGCTGGTGGA GACGGAGAGG CGCGGCCAAG 
GCGGGGCCCT CTGGGTGTCG GCGGTGGGGT CAAGCCATCT CAAAATCCAA ACTCAGCCGA 
CGCTGGCGTC GCTGGAACCG ATTCAATCGC AGAAGATGTA GGGCCGCCGT GAAGTCTGTC 
ACGTTTTACT GGCTGGTTAT CGTCCTGGTG TTTCTGAACA CCTTAACCAT TTCCTCTGAG 
CACTACAATC AGCCAGATTG GTTGACACAG ATTCAAGATA TTGCCAACAA AGTCCTCTTG 
GCTCTGTTCA CCTGCGAGAT GCTGGTAAAA ATGTACAGCT TGGGCCTCCA AGCATATTTC 
GTCTCTCTTT TCAACCGGTT TGATTGCTTC GTGGTGTGTG GTGGAATCAC TGAGACGATC 
TTGGTGGAAC TGGAAATCAT GTCTCCCCTG GGGATCTCTG TGTTTCGGTG TGTGCGCCTC 
TTAAGAATCT TCAAAGTGAC CAGGCACTGG ACTTCCCTGA GCAACTTAGT GGCATCCTTA 
TTAAACTCCA TGAAGTCCAT CGCTTCGCTG TTGCTTCTGC TTTTTCTCTT CATTATCATC 
TTTTCCTTGC TTGGGATGCA GCTGTTTGGC GGCAAGTTTA ATTTTGATGA AACGCAAACC 
AAGCGGAGCA CCTTTGACAA TTTCCCTCAA GCACTTCTCA CAGTGTTCCA GATCCTGACA 
GGCGAAGACT GGAATGCTGT GATGTACGAT GGCATCATGG CTTACGGGGG CCCATCCTCT 
TCAGGAATGA TCGTCTGCAT CTACTTCATC ATCCTCTTCA TTTGTGGTAA CTATATTCTA 
CTGAATGTCT TCTTGGCCAT CGCTGTAGAC AATTTGGCTG ATGCTGAAAG TCTGAACACT 
GCTCAGAAAG AAGAAGCGGA AGAAAAGGAG AGGAAAAAGA TTGCCAGAAA AGAGAGCCTA 
GAAAATAAAA AGAACAACAA ACCAGAAGTC AACCAGATAG CCAACAGTGA CAACAAGGTT 
ACAATTGATG ACTATAGAGA AGAGGATGAA GACAAGGACC CCTATCCGCC TTGCGATGTG 
CCAGTAGGGG AAGAGGAAGA GGAAGAGGAG GAGGATGAAC CTGAGGTTCC TGCCGGACCC 
CGTCCTCGAA GGATCTCGGA GTTGAACATG AAGGAAAAAA TTGCCCCCAT CCCTGAAGGG 
AGCGCTTTCT TCATTCTTAG CAAGACCAAC CCGATCCGCG TAGGCTGCCA CAAGCTCATC 
AACCACCACA TCTTCACCAA CCTCATCCTT GTCTTCATCA TGCTGAGCAG CGCTGCCCTG 
GCCGCAGAGG ACCCCATCCG CAGCCACTCC TTCCGGAACA CGATACTGGG TTACTTTGAC 
TATGCCTTCA CAGCCATCTT TACTGTTGAG ATCCTGTTGA AGATGACAAC TTTTGGAGCT 
TTCCTCCACA AAGGGGCCTT CTGCAGGAAC TACTTCAATT TGCTGGATAT GCTGGTGGTT 
GGGGTGTCTC TGGTGTCATT TGGGATTCAA TCCAGTGCCA TCTCCGTTGT GAAGATTCTG 
AGGGTCTTAA GGGTCCTGCG TCCCCTCAGG GCCATCAACA GAGCAAAAGG ACTTAAGCAC 
GTGGTCCAGT GCGTCTTCGT GGCCATCCGG ACCATCGGCA ACATCATGAT CGTCACCACC 
CTCCTGCAGT TCATGTTTGC CTGTATCGGG GTCCAGTTGT TCAAGGGGAA GTTCTATCGC 
TGTACGGATG AAGCCAAAAG TAACCCTGAA GAATGCAGGG GACTTTTCAT CCTCTACAAG 
GATGGGGATG TTGACAGTCC TGTGGTCCGT GAACGGATCT GGCAAAACAG TGATTTCAAC 
TTCGACAACG TCCTCTCTGC TATGATGGCG CTCTTCACAG TCTCCACGTT TGAGGGCTGG 
CCTGCGTTGC TGTATAAAGC CATCGACTCG AATGGAGAGA ACATCGGCCC AATCTACAAC 
CACCGCGTGG AGATCTCCAT CTTCTTCATC ATCTACATCA TCATTGTAGC TTTCTTCATG 
ATGAACATCT TTGTGGGCTT TGTCATCGTT ACATTTCAGG AACAAGGAGA AAAAGAGTAT 
AAGAACTGTG AGCTGGACAA AAATCAGCGT CAGTGTGTTG AATACGCCTT GAAAGCACGT 
CCCTTGCGGA GATACATCCC CAAAAACCCC TACCAGTACA AGTTCTGGTA CGTGGTGAAC 
TCTTCGCCTT TCGAATACAT GATGTTTGTC CTCATCATGC TCAACACACT CTGCTTGGCC 
ATGCAGCACT ACGAGCAGTC CAAGATGTTC AATGATGCCA TGGACATTCT GAACATGGTC 
TTCACCGGGG TGTTCACCGT CGAGATGGTT TTGAAAGTCA TCGCATTTAA GCCTAAGGGG 
TATTTTAGTG ACGCCTGGAA CACGTTTGAC TCCCTCATCG TAATCGGCAG CATTATAGAC 
GTGGCCCTCA GCGAAGCAGA CCACTATTTC ACTGATGCAT GGAACACTTT TGATGCCTTA 
ATTGTTGTTG GTAGCGTCGT TGATATTGCT ATAACTGAAG TGAATCCAAC TGAAAGTGAA 
AATGTCCCTG TCCCAACTGC TACACCTGGG AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGA TTGGTGAAGC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACAT TGCTGTGGAC TTTTATTAAG TCCTTTCAGG CGCTCCCGTA TGTGGCCCTC 
CTCATAGCCA TGCTGTTCTT CATCTATGCG GTCATTGGCA TGCAGATGTT TGGGAAAGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAATAACT TCCAGACGTT TCCCCAGGCG 
GTGCTGCTGC TCTTCAGGTG TGCAACAGGT GAGGCCTGGC AGGAGATCAT GCTGGCCTGT 
CTCCCAGGGA AGCTCTGTGA CCCTGAGTCA GATTACAACC CCGGGGAGGA GTATACATGT 
GGGAGCAACT TTGCCATTGT CTATTTCATC AGTTTTTACA TGCTCTGTGC ATTTCTGATC 
ATCAATCTGT TTGTGGCTGT CATCATGGAT AATTTCGACT ATCTGACCCG GGACTGGTCT 
ATTTTGGGGC CTCACCATTT AGATGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAG 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTCTGC TTCGACGCAT CCAGCCTCCC 
CTGGGGTTTG GGAAGTTATG TCCACACAGG GTAGCGTGCA AGAGATTAGT TGCCATGAAC 
ATGCCTCTCA ACAGTGACGG GACAGTCATG TTTAATGCAA CCCTGTTTGC TTTGGTTCGA 
ACGGCTCTTA AGATCAAGAC CGAAGGGAAC CTGGAGCAAG CTAATGAAGA ACTTCGGGCT 
GTGATAAAGA AAATTTGGAA GAAAACCAGC ATGAAATTAC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAGTACCC TGCGAAGAAC 
ACCACAATTG CCCTACAGGC GGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CGTGTGATTT GCAAGATGAC GAGCCTGAGG AAACAAAACG AGAAGAAGAA 
GATGATGTGT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ATTCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAATTC CACCTGCAAG TGATACTGAG AAACCGCTGT TTCCTCCAGC AGGAAATTCG 
GTGTGTCATA ACCATCATAA CCATAATTCC ATAGGAAAGC AAGTTCCCAC CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTCCAAA GCTGCCCATG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC ATGTGTCTGA AAATGGGCAT CATTCTTCCC ACAAGCATGA CCGGGAGCCT 
CAGAGAAGGT CCAGTGTGAA AAGAACCCGC TATTATGAAA CTTACATTAG GTCCGACTCA 
GGAGATGAAC AGCTCCCAAC TATTTGCCGG GAAGACCCAG AGATACATGG CTATTTCAGG 
GACCCCCACT GCTTGGGGGA GCAGGAGTAT TTCAGTAGTG AGGAATGCTA CGAGGATGAC 
AGCTCGCCCA CCTGGAGCAG GCAAAACTAT GGCTACTACA GCAGATACCC AGGCAGAAAC 
ATCGACTCTG AGAGGCCCCG AGGCTACCAT CATCCCCAAG GATTCTTGGA GGACGATGAC 
TCGCCCGTTT GCTATGATTC ACGGAGATCT CCAAGGAGAC GCCTACTACC TCCCACCCCA 
GCATCCCACC GGAGATCCTC CTTCAACTTT GAGTGCCTGC GCCGGCAGAG CAGCCAGGAA 
GAGGTCCCGT CGTCTCCCAT CTTCCCCCAT CGCACGGCCC TGCCTCTGCA TCTAATGCAG 
CAACAGATCA TGGCAGTTGC CGGCCTAGAT TCAAGTAAAG CCCAGAAGTA CTCACCGAGT 
CACTCGACCC GGTCGTGGGC CACCCCTCCA GCAACCCCTC CCTACCGGGA CTGGACACCG 
TGCTACACCC CCCTGATCCA AGTGGAGCAG TCAGAGGCCC TGGACCAGGT GAACGGCAGC 
CTGCCGTCCC TGCACCGCAG CTCCTGGTAC ACAGACGAGC CCGACATCTC CTACCGGACT 
TTCACACCAG CCAGCCTGAC TGTCCCCAGC AGCTTCCGGA ACAAAAACAG CGACAAGCAG 
AGGAGTGCGG ACAGCTTGGT GGAGGCAGTC CTGATATCCG AAGGCTTGGG ACGCTATGCA 
AGGGACCCAA AATTTGTGTC AGCAACAAAA CACGAAATCG CTGATGCCTG TGACCTCACC 
ATCGACGAGA TGGAGAGTGC AGCCAGCACC CTGCTTAATG GGAACGTGCG TCCCCGAGCC 
AACGGGGATG TGGGCCCCCT CTCACACCGG CAGGACTATG AGCTACAGGA CTTTGGTCCT 
GGCTACAGCG ACGAAGAGCC AGACCCTGGG AGGGATGAGG AGGACCTGGC GGATGAAATG 
ATATGCATCA CCACCTTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_017007138.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862627.1 Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X3, mRNA.

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CloneID	OHu94540
Clone ID Related Accession (Same CDS sequence)	XM_017007138.1
Accession Version	XM_017007138.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6738bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGGG AGCCGCTGCC GCTGTGGGGC TCCCCTCCCC AGCTCTGGCC CGGGCACCAC 
GTGCAGCTTC CACGCCGGCC GGGGCTGGGT CGAGGGAGGA TGGAGCAGCT CCTGGCCAGC 
CCTCCCCTTT GGAGACCGCC AGTGCCCCCC AACCCCTGTC GGCTGGAAGC CTTTCCGGAG 
GCAAACTATG CAAGAGGCAC CAGACTTCCT CTTTCTGGTG AAGGACCAAC TTCTCAGCCG 
AATAGCTCCA AGCAAACTGT CCTGTCTTGG CAAGCTGCAA TCGATGCTGC TAGACAGGCC 
AAGGCTGCCC AAACTATGAG CACCTCTGCA CCCCCACCTG TAGGATCTCT CTCCCAAAGA 
AAACGTCAGC AATACGCCAA GAGCAAAAAA CAGGGTAACT CGTCCAACAG CCGACCTGCC 
CGCGCCCTTT TCTGTTTATC ACTCAATAAC CCCATCCGAA GAGCCTGCAT TAGTATAGTG 
GAATGGAAAC CATTTGACAT ATTTATATTA TTGGCTATTT TTGCCAATTG TGTGGCCTTA 
GCTATTTACA TCCCATTCCC TGAAGATGAT TCTAATTCAA CAAATCATAA CTTGGAAAAA 
GTAGAATATG CCTTCCTGAT TATTTTTACA GTCGAGACAT TTTTGAAGAT TATAGCGTAT 
GGATTATTGC TACATCCTAA TGCTTATGTT AGGAATGGAT GGAATTTACT GGATTTTGTT 
ATAGTAATAG TAGGATTGTT TAGTGTAATT TTGGAACAAT TAACCAAAGA AACAGAAGGC 
GGGAACCACT CAAGCGGCAA ATCTGGAGGC TTTGATGTCA AAGCCCTCCG TGCCTTTCGA 
GTGTTGCGAC CACTTCGACT AGTGTCAGGA GTGCCCAGTT TACAAGTTGT CCTGAACTCC 
ATTATAAAAG CCATGGTTCC CCTCCTTCAC ATAGCCCTTT TGGTATTATT TGTAATCATA 
ATCTATGCTA TTATAGGATT GGAACTTTTT ATTGGAAAAA TGCACAAAAC ATGTTTTTTT 
GCTGACTCAG ATATCGTAGC TGAAGAGGAC CCAGCTCCAT GTGCGTTCTC AGGGAATGGA 
CGCCAGTGTA CTGCCAATGG CACGGAATGT AGGAGTGGCT GGGTTGGCCC GAACGGAGGC 
ATCACCAACT TTGATAACTT TGCCTTTGCC ATGCTTACTG TGTTTCAGTG CATCACCATG 
GAGGGCTGGA CAGATGTGCT CTACTGGGTA AATGATGCGA TAGGATGGGA ATGGCCATGG 
GTGTATTTTG TTAGTCTGAT CATCCTTGGC TCATTTTTCG TCCTTAACCT GGTTCTTGGT 
GTCCTTAGTG GAGAATTCTC AAAGGAAAGA GAGAAGGCAA AAGCACGGGG AGATTTCCAG 
AAGCTCCGGG AGAAGCAGCA GCTGGAGGAG GATCTAAAGG GCTACTTGGA TTGGATCACC 
CAAGCTGAGG ACATCGATCC GGAGAATGAG GAAGAAGGAG GAGAGGAAGG CAAACGAAAT 
ACTAGCATGC CCACCAGCGA GACTGAGTCT GTGAACACAG AGAACGTCAG CGGTGAAGGC 
GAGAACCGAG GCTGCTGTGG AAGTCTCTGG TGCTGGTGGA GACGGAGAGG CGCGGCCAAG 
GCGGGGCCCT CTGGGTGTCG GCGGTGGGGT CAAGCCATCT CAAAATCCAA ACTCAGCCGA 
CGCTGGCGTC GCTGGAACCG ATTCAATCGC AGAAGATGTA GGGCCGCCGT GAAGTCTGTC 
ACGTTTTACT GGCTGGTTAT CGTCCTGGTG TTTCTGAACA CCTTAACCAT TTCCTCTGAG 
CACTACAATC AGCCAGATTG GTTGACACAG ATTCAAGATA TTGCCAACAA AGTCCTCTTG 
GCTCTGTTCA CCTGCGAGAT GCTGGTAAAA ATGTACAGCT TGGGCCTCCA AGCATATTTC 
GTCTCTCTTT TCAACCGGTT TGATTGCTTC GTGGTGTGTG GTGGAATCAC TGAGACGATC 
TTGGTGGAAC TGGAAATCAT GTCTCCCCTG GGGATCTCTG TGTTTCGGTG TGTGCGCCTC 
TTAAGAATCT TCAAAGTGAC CAGGCACTGG ACTTCCCTGA GCAACTTAGT GGCATCCTTA 
TTAAACTCCA TGAAGTCCAT CGCTTCGCTG TTGCTTCTGC TTTTTCTCTT CATTATCATC 
TTTTCCTTGC TTGGGATGCA GCTGTTTGGC GGCAAGTTTA ATTTTGATGA AACGCAAACC 
AAGCGGAGCA CCTTTGACAA TTTCCCTCAA GCACTTCTCA CAGTGTTCCA GATCCTGACA 
GGCGAAGACT GGAATGCTGT GATGTACGAT GGCATCATGG CTTACGGGGG CCCATCCTCT 
TCAGGAATGA TCGTCTGCAT CTACTTCATC ATCCTCTTCA TTTGTGGTAA CTATATTCTA 
CTGAATGTCT TCTTGGCCAT CGCTGTAGAC AATTTGGCTG ATGCTGAAAG TCTGAACACT 
GCTCAGAAAG AAGAAGCGGA AGAAAAGGAG AGGAAAAAGA TTGCCAGAAA AGAGAGCCTA 
GAAAATAAAA AGAACAACAA ACCAGAAGTC AACCAGATAG CCAACAGTGA CAACAAGGTT 
ACAATTGATG ACTATAGAGA AGAGGATGAA GACAAGGACC CCTATCCGCC TTGCGATGTG 
CCAGGGGAAG AGGAAGAGGA AGAGGAGGAG GATGAACCTG AGGTTCCTGC CGGACCCCGT 
CCTCGAAGGA TCTCGGAGTT GAACATGAAG GAAAAAATTG CCCCCATCCC TGAAGGGAGC 
GCTTTCTTCA TTCTTAGCAA GACCAACCCG ATCCGCGTAG GCTGCCACAA GCTCATCAAC 
CACCACATCT TCACCAACCT CATCCTTGTC TTCATCATGC TGAGCAGCGC TGCCCTGGCC 
GCAGAGGACC CCATCCGCAG CCACTCCTTC CGGAACACGA TACTGGGTTA CTTTGACTAT 
GCCTTCACAG CCATCTTTAC TGTTGAGATC CTGTTGAAGA TGACAACTTT TGGAGCTTTC 
CTCCACAAAG GGGCCTTCTG CAGGAACTAC TTCAATTTGC TGGATATGCT GGTGGTTGGG 
GTGTCTCTGG TGTCATTTGG GATTCAATCC AGTGCCATCT CCGTTGTGAA GATTCTGAGG 
GTCTTAAGGG TCCTGCGTCC CCTCAGGGCC ATCAACAGAG CAAAAGGACT TAAGCACGTG 
GTCCAGTGCG TCTTCGTGGC CATCCGGACC ATCGGCAACA TCATGATCGT CACCACCCTC 
CTGCAGTTCA TGTTTGCCTG TATCGGGGTC CAGTTGTTCA AGGGGAAGTT CTATCGCTGT 
ACGGATGAAG CCAAAAGTAA CCCTGAAGAA TGCAGGGGAC TTTTCATCCT CTACAAGGAT 
GGGGATGTTG ACAGTCCTGT GGTCCGTGAA CGGATCTGGC AAAACAGTGA TTTCAACTTC 
GACAACGTCC TCTCTGCTAT GATGGCGCTC TTCACAGTCT CCACGTTTGA GGGCTGGCCT 
GCGTTGCTGT ATAAAGCCAT CGACTCGAAT GGAGAGAACA TCGGCCCAAT CTACAACCAC 
CGCGTGGAGA TCTCCATCTT CTTCATCATC TACATCATCA TTGTAGCTTT CTTCATGATG 
AACATCTTTG TGGGCTTTGT CATCGTTACA TTTCAGGAAC AAGGAGAAAA AGAGTATAAG 
AACTGTGAGC TGGACAAAAA TCAGCGTCAG TGTGTTGAAT ACGCCTTGAA AGCACGTCCC 
TTGCGGAGAT ACATCCCCAA AAACCCCTAC CAGTACAAGT TCTGGTACGT GGTGAACTCT 
TCGCCTTTCG AATACATGAT GTTTGTCCTC ATCATGCTCA ACACACTCTG CTTGGCCATG 
CAGCACTACG AGCAGTCCAA GATGTTCAAT GATGCCATGG ACATTCTGAA CATGGTCTTC 
ACCGGGGTGT TCACCGTCGA GATGGTTTTG AAAGTCATCG CATTTAAGCC TAAGGGGTAT 
TTTAGTGACG CCTGGAACAC GTTTGACTCC CTCATCGTAA TCGGCAGCAT TATAGACGTG 
GCCCTCAGCG AAGCAGACCA CTATTTCACT GATGCATGGA ACACTTTTGA TGCCTTAATT 
GTTGTTGGTA GCGTCGTTGA TATTGCTATA ACTGAAGTGA ATCCAACTGA AAGTGAAAAT 
GTCCCTGTCC CAACTGCTAC ACCTGGGAAC TCTGAAGAGA GCAATAGAAT CTCCATCACC 
TTTTTCCGTC TTTTCCGAGT GATGCGATTG GTGAAGCTTC TCAGCAGGGG GGAAGGCATC 
CGGACATTGC TGTGGACTTT TATTAAGTCC TTTCAGGCGC TCCCGTATGT GGCCCTCCTC 
ATAGCCATGC TGTTCTTCAT CTATGCGGTC ATTGGCATGC AGATGTTTGG GAAAGTTGCC 
ATGAGAGATA ACAACCAGAT CAATAGGAAC AATAACTTCC AGACGTTTCC CCAGGCGGTG 
CTGCTGCTCT TCAGGTGTGC AACAGGTGAG GCCTGGCAGG AGATCATGCT GGCCTGTCTC 
CCAGGGAAGC TCTGTGACCC TGAGTCAGAT TACAACCCCG GGGAGGAGTA TACATGTGGG 
AGCAACTTTG CCATTGTCTA TTTCATCAGT TTTTACATGC TCTGTGCATT TCTGATCATC 
AATCTGTTTG TGGCTGTCAT CATGGATAAT TTCGACTATC TGACCCGGGA CTGGTCTATT 
TTGGGGCCTC ACCATTTAGA TGAATTCAAA AGAATATGGT CAGAATATGA CCCTGAGGCA 
AAGGGAAGGA TAAAACACCT TGATGTGGTC ACTCTGCTTC GACGCATCCA GCCTCCCCTG 
GGGTTTGGGA AGTTATGTCC ACACAGGGTA GCGTGCAAGA GATTAGTTGC CATGAACATG 
CCTCTCAACA GTGACGGGAC AGTCATGTTT AATGCAACCC TGTTTGCTTT GGTTCGAACG 
GCTCTTAAGA TCAAGACCGA AGGGAACCTG GAGCAAGCTA ATGAAGAACT TCGGGCTGTG 
ATAAAGAAAA TTTGGAAGAA AACCAGCATG AAATTACTTG ACCAAGTTGT CCCTCCAGCT 
GGTGATGATG AGGTAACCGT GGGGAAGTTC TATGCCACTT TCCTGATACA GGACTACTTT 
AGGAAATTCA AGAAACGGAA AGAACAAGGA CTGGTGGGAA AGTACCCTGC GAAGAACACC 
ACAATTGCCC TACAGGCGGG ATTAAGGACA CTGCATGACA TTGGGCCAGA AATCCGGCGT 
GCTATATCGT GTGATTTGCA AGATGACGAG CCTGAGGAAA CAAAACGAGA AGAAGAAGAT 
GATGTGTTCA AAAGAAATGG TGCCCTGCTT GGAAACCATG TCAATCATGT TAATAGTGAT 
AGGAGAGATT CCCTTCAGCA GACCAATACC ACCCACCGTC CCCTGCATGT CCAAAGGCCT 
TCAATTCCAC CTGCAAGTGA TACTGAGAAA CCGCTGTTTC CTCCAGCAGG AAATTCGGTG 
TGTCATAACC ATCATAACCA TAATTCCATA GGAAAGCAAG TTCCCACCTC AACAAATGCC 
AATCTCAATA ATGCCAATAT GTCCAAAGCT GCCCATGGAA AGCGGCCCAG CATTGGGAAC 
CTTGAGCATG TGTCTGAAAA TGGGCATCAT TCTTCCCACA AGCATGACCG GGAGCCTCAG 
AGAAGGTCCA GTGTGAAAAG AACCCGCTAT TATGAAACTT ACATTAGGTC CGACTCAGGA 
GATGAACAGC TCCCAACTAT TTGCCGGGAA GACCCAGAGA TACATGGCTA TTTCAGGGAC 
CCCCACTGCT TGGGGGAGCA GGAGTATTTC AGTAGTGAGG AATGCTACGA GGATGACAGC 
TCGCCCACCT GGAGCAGGCA AAACTATGGC TACTACAGCA GATACCCAGG CAGAAACATC 
GACTCTGAGA GGCCCCGAGG CTACCATCAT CCCCAAGGAT TCTTGGAGGA CGATGACTCG 
CCCGTTTGCT ATGATTCACG GAGATCTCCA AGGAGACGCC TACTACCTCC CACCCCAGCA 
TCCCACCGGA GATCCTCCTT CAACTTTGAG TGCCTGCGCC GGCAGAGCAG CCAGGAAGAG 
GTCCCGTCGT CTCCCATCTT CCCCCATCGC ACGGCCCTGC CTCTGCATCT AATGCAGCAA 
CAGATCATGG CAGTTGCCGG CCTAGATTCA AGTAAAGCCC AGAAGTACTC ACCGAGTCAC 
TCGACCCGGT CGTGGGCCAC CCCTCCAGCA ACCCCTCCCT ACCGGGACTG GACACCGTGC 
TACACCCCCC TGATCCAAGT GGAGCAGTCA GAGGCCCTGG ACCAGGTGAA CGGCAGCCTG 
CCGTCCCTGC ACCGCAGCTC CTGGTACACA GACGAGCCCG ACATCTCCTA CCGGACTTTC 
ACACCAGCCA GCCTGACTGT CCCCAGCAGC TTCCGGAACA AAAACAGCGA CAAGCAGAGG 
AGTGCGGACA GCTTGGTGGA GGCAGTCCTG ATATCCGAAG GCTTGGGACG CTATGCAAGG 
GACCCAAAAT TTGTGTCAGC AACAAAACAC GAAATCGCTG ATGCCTGTGA CCTCACCATC 
GACGAGATGG AGAGTGCAGC CAGCACCCTG CTTAATGGGA ACGTGCGTCC CCGAGCCAAC 
GGGGATGTGG GCCCCCTCTC ACACCGGCAG GACTATGAGC TACAGGACTT TGGTCCTGGC 
TACAGCGACG AAGAGCCAGA CCCTGGGAGG GATGAGGAGG ACCTGGCGGA TGAAATGATA 
TGCATCACCA CCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862627.1
CDS	16..6753
Translation	MPGEPLPLWGSPPQLWPGHHVQLPRRPGLGRGRMEQLLASPPLWRPPVPPNPCRLEAFPEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGENRGCCGSLWCWWRRRGAAKAGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENIGPIYNHRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPTESENVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEDDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGNLEHVSENGHHSSHKHDREPQRRSSVKRTRYYETYIRSDSGDEQLPTICREDPEIHGYFRDPHCLGEQEYFSSEECYEDDSSPTWSRQNYGYYSRYPGRNIDSERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPASHRRSSFNFECLRRQSSQEEVPSSPIFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVEQSEALDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGNVRPRANGDVGPLSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017007138.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007138.1

ORF Insert Sequence:

ATGCCCGGGG AGCCGCTGCC GCTGTGGGGC TCCCCTCCCC AGCTCTGGCC CGGGCACCAC 
GTGCAGCTTC CACGCCGGCC GGGGCTGGGT CGAGGGAGGA TGGAGCAGCT CCTGGCCAGC 
CCTCCCCTTT GGAGACCGCC AGTGCCCCCC AACCCCTGTC GGCTGGAAGC CTTTCCGGAG 
GCAAACTATG CAAGAGGCAC CAGACTTCCT CTTTCTGGTG AAGGACCAAC TTCTCAGCCG 
AATAGCTCCA AGCAAACTGT CCTGTCTTGG CAAGCTGCAA TCGATGCTGC TAGACAGGCC 
AAGGCTGCCC AAACTATGAG CACCTCTGCA CCCCCACCTG TAGGATCTCT CTCCCAAAGA 
AAACGTCAGC AATACGCCAA GAGCAAAAAA CAGGGTAACT CGTCCAACAG CCGACCTGCC 
CGCGCCCTTT TCTGTTTATC ACTCAATAAC CCCATCCGAA GAGCCTGCAT TAGTATAGTG 
GAATGGAAAC CATTTGACAT ATTTATATTA TTGGCTATTT TTGCCAATTG TGTGGCCTTA 
GCTATTTACA TCCCATTCCC TGAAGATGAT TCTAATTCAA CAAATCATAA CTTGGAAAAA 
GTAGAATATG CCTTCCTGAT TATTTTTACA GTCGAGACAT TTTTGAAGAT TATAGCGTAT 
GGATTATTGC TACATCCTAA TGCTTATGTT AGGAATGGAT GGAATTTACT GGATTTTGTT 
ATAGTAATAG TAGGATTGTT TAGTGTAATT TTGGAACAAT TAACCAAAGA AACAGAAGGC 
GGGAACCACT CAAGCGGCAA ATCTGGAGGC TTTGATGTCA AAGCCCTCCG TGCCTTTCGA 
GTGTTGCGAC CACTTCGACT AGTGTCAGGA GTGCCCAGTT TACAAGTTGT CCTGAACTCC 
ATTATAAAAG CCATGGTTCC CCTCCTTCAC ATAGCCCTTT TGGTATTATT TGTAATCATA 
ATCTATGCTA TTATAGGATT GGAACTTTTT ATTGGAAAAA TGCACAAAAC ATGTTTTTTT 
GCTGACTCAG ATATCGTAGC TGAAGAGGAC CCAGCTCCAT GTGCGTTCTC AGGGAATGGA 
CGCCAGTGTA CTGCCAATGG CACGGAATGT AGGAGTGGCT GGGTTGGCCC GAACGGAGGC 
ATCACCAACT TTGATAACTT TGCCTTTGCC ATGCTTACTG TGTTTCAGTG CATCACCATG 
GAGGGCTGGA CAGATGTGCT CTACTGGGTA AATGATGCGA TAGGATGGGA ATGGCCATGG 
GTGTATTTTG TTAGTCTGAT CATCCTTGGC TCATTTTTCG TCCTTAACCT GGTTCTTGGT 
GTCCTTAGTG GAGAATTCTC AAAGGAAAGA GAGAAGGCAA AAGCACGGGG AGATTTCCAG 
AAGCTCCGGG AGAAGCAGCA GCTGGAGGAG GATCTAAAGG GCTACTTGGA TTGGATCACC 
CAAGCTGAGG ACATCGATCC GGAGAATGAG GAAGAAGGAG GAGAGGAAGG CAAACGAAAT 
ACTAGCATGC CCACCAGCGA GACTGAGTCT GTGAACACAG AGAACGTCAG CGGTGAAGGC 
GAGAACCGAG GCTGCTGTGG AAGTCTCTGG TGCTGGTGGA GACGGAGAGG CGCGGCCAAG 
GCGGGGCCCT CTGGGTGTCG GCGGTGGGGT CAAGCCATCT CAAAATCCAA ACTCAGCCGA 
CGCTGGCGTC GCTGGAACCG ATTCAATCGC AGAAGATGTA GGGCCGCCGT GAAGTCTGTC 
ACGTTTTACT GGCTGGTTAT CGTCCTGGTG TTTCTGAACA CCTTAACCAT TTCCTCTGAG 
CACTACAATC AGCCAGATTG GTTGACACAG ATTCAAGATA TTGCCAACAA AGTCCTCTTG 
GCTCTGTTCA CCTGCGAGAT GCTGGTAAAA ATGTACAGCT TGGGCCTCCA AGCATATTTC 
GTCTCTCTTT TCAACCGGTT TGATTGCTTC GTGGTGTGTG GTGGAATCAC TGAGACGATC 
TTGGTGGAAC TGGAAATCAT GTCTCCCCTG GGGATCTCTG TGTTTCGGTG TGTGCGCCTC 
TTAAGAATCT TCAAAGTGAC CAGGCACTGG ACTTCCCTGA GCAACTTAGT GGCATCCTTA 
TTAAACTCCA TGAAGTCCAT CGCTTCGCTG TTGCTTCTGC TTTTTCTCTT CATTATCATC 
TTTTCCTTGC TTGGGATGCA GCTGTTTGGC GGCAAGTTTA ATTTTGATGA AACGCAAACC 
AAGCGGAGCA CCTTTGACAA TTTCCCTCAA GCACTTCTCA CAGTGTTCCA GATCCTGACA 
GGCGAAGACT GGAATGCTGT GATGTACGAT GGCATCATGG CTTACGGGGG CCCATCCTCT 
TCAGGAATGA TCGTCTGCAT CTACTTCATC ATCCTCTTCA TTTGTGGTAA CTATATTCTA 
CTGAATGTCT TCTTGGCCAT CGCTGTAGAC AATTTGGCTG ATGCTGAAAG TCTGAACACT 
GCTCAGAAAG AAGAAGCGGA AGAAAAGGAG AGGAAAAAGA TTGCCAGAAA AGAGAGCCTA 
GAAAATAAAA AGAACAACAA ACCAGAAGTC AACCAGATAG CCAACAGTGA CAACAAGGTT 
ACAATTGATG ACTATAGAGA AGAGGATGAA GACAAGGACC CCTATCCGCC TTGCGATGTG 
CCAGGGGAAG AGGAAGAGGA AGAGGAGGAG GATGAACCTG AGGTTCCTGC CGGACCCCGT 
CCTCGAAGGA TCTCGGAGTT GAACATGAAG GAAAAAATTG CCCCCATCCC TGAAGGGAGC 
GCTTTCTTCA TTCTTAGCAA GACCAACCCG ATCCGCGTAG GCTGCCACAA GCTCATCAAC 
CACCACATCT TCACCAACCT CATCCTTGTC TTCATCATGC TGAGCAGCGC TGCCCTGGCC 
GCAGAGGACC CCATCCGCAG CCACTCCTTC CGGAACACGA TACTGGGTTA CTTTGACTAT 
GCCTTCACAG CCATCTTTAC TGTTGAGATC CTGTTGAAGA TGACAACTTT TGGAGCTTTC 
CTCCACAAAG GGGCCTTCTG CAGGAACTAC TTCAATTTGC TGGATATGCT GGTGGTTGGG 
GTGTCTCTGG TGTCATTTGG GATTCAATCC AGTGCCATCT CCGTTGTGAA GATTCTGAGG 
GTCTTAAGGG TCCTGCGTCC CCTCAGGGCC ATCAACAGAG CAAAAGGACT TAAGCACGTG 
GTCCAGTGCG TCTTCGTGGC CATCCGGACC ATCGGCAACA TCATGATCGT CACCACCCTC 
CTGCAGTTCA TGTTTGCCTG TATCGGGGTC CAGTTGTTCA AGGGGAAGTT CTATCGCTGT 
ACGGATGAAG CCAAAAGTAA CCCTGAAGAA TGCAGGGGAC TTTTCATCCT CTACAAGGAT 
GGGGATGTTG ACAGTCCTGT GGTCCGTGAA CGGATCTGGC AAAACAGTGA TTTCAACTTC 
GACAACGTCC TCTCTGCTAT GATGGCGCTC TTCACAGTCT CCACGTTTGA GGGCTGGCCT 
GCGTTGCTGT ATAAAGCCAT CGACTCGAAT GGAGAGAACA TCGGCCCAAT CTACAACCAC 
CGCGTGGAGA TCTCCATCTT CTTCATCATC TACATCATCA TTGTAGCTTT CTTCATGATG 
AACATCTTTG TGGGCTTTGT CATCGTTACA TTTCAGGAAC AAGGAGAAAA AGAGTATAAG 
AACTGTGAGC TGGACAAAAA TCAGCGTCAG TGTGTTGAAT ACGCCTTGAA AGCACGTCCC 
TTGCGGAGAT ACATCCCCAA AAACCCCTAC CAGTACAAGT TCTGGTACGT GGTGAACTCT 
TCGCCTTTCG AATACATGAT GTTTGTCCTC ATCATGCTCA ACACACTCTG CTTGGCCATG 
CAGCACTACG AGCAGTCCAA GATGTTCAAT GATGCCATGG ACATTCTGAA CATGGTCTTC 
ACCGGGGTGT TCACCGTCGA GATGGTTTTG AAAGTCATCG CATTTAAGCC TAAGGGGTAT 
TTTAGTGACG CCTGGAACAC GTTTGACTCC CTCATCGTAA TCGGCAGCAT TATAGACGTG 
GCCCTCAGCG AAGCAGACCA CTATTTCACT GATGCATGGA ACACTTTTGA TGCCTTAATT 
GTTGTTGGTA GCGTCGTTGA TATTGCTATA ACTGAAGTGA ATCCAACTGA AAGTGAAAAT 
GTCCCTGTCC CAACTGCTAC ACCTGGGAAC TCTGAAGAGA GCAATAGAAT CTCCATCACC 
TTTTTCCGTC TTTTCCGAGT GATGCGATTG GTGAAGCTTC TCAGCAGGGG GGAAGGCATC 
CGGACATTGC TGTGGACTTT TATTAAGTCC TTTCAGGCGC TCCCGTATGT GGCCCTCCTC 
ATAGCCATGC TGTTCTTCAT CTATGCGGTC ATTGGCATGC AGATGTTTGG GAAAGTTGCC 
ATGAGAGATA ACAACCAGAT CAATAGGAAC AATAACTTCC AGACGTTTCC CCAGGCGGTG 
CTGCTGCTCT TCAGGTGTGC AACAGGTGAG GCCTGGCAGG AGATCATGCT GGCCTGTCTC 
CCAGGGAAGC TCTGTGACCC TGAGTCAGAT TACAACCCCG GGGAGGAGTA TACATGTGGG 
AGCAACTTTG CCATTGTCTA TTTCATCAGT TTTTACATGC TCTGTGCATT TCTGATCATC 
AATCTGTTTG TGGCTGTCAT CATGGATAAT TTCGACTATC TGACCCGGGA CTGGTCTATT 
TTGGGGCCTC ACCATTTAGA TGAATTCAAA AGAATATGGT CAGAATATGA CCCTGAGGCA 
AAGGGAAGGA TAAAACACCT TGATGTGGTC ACTCTGCTTC GACGCATCCA GCCTCCCCTG 
GGGTTTGGGA AGTTATGTCC ACACAGGGTA GCGTGCAAGA GATTAGTTGC CATGAACATG 
CCTCTCAACA GTGACGGGAC AGTCATGTTT AATGCAACCC TGTTTGCTTT GGTTCGAACG 
GCTCTTAAGA TCAAGACCGA AGGGAACCTG GAGCAAGCTA ATGAAGAACT TCGGGCTGTG 
ATAAAGAAAA TTTGGAAGAA AACCAGCATG AAATTACTTG ACCAAGTTGT CCCTCCAGCT 
GGTGATGATG AGGTAACCGT GGGGAAGTTC TATGCCACTT TCCTGATACA GGACTACTTT 
AGGAAATTCA AGAAACGGAA AGAACAAGGA CTGGTGGGAA AGTACCCTGC GAAGAACACC 
ACAATTGCCC TACAGGCGGG ATTAAGGACA CTGCATGACA TTGGGCCAGA AATCCGGCGT 
GCTATATCGT GTGATTTGCA AGATGACGAG CCTGAGGAAA CAAAACGAGA AGAAGAAGAT 
GATGTGTTCA AAAGAAATGG TGCCCTGCTT GGAAACCATG TCAATCATGT TAATAGTGAT 
AGGAGAGATT CCCTTCAGCA GACCAATACC ACCCACCGTC CCCTGCATGT CCAAAGGCCT 
TCAATTCCAC CTGCAAGTGA TACTGAGAAA CCGCTGTTTC CTCCAGCAGG AAATTCGGTG 
TGTCATAACC ATCATAACCA TAATTCCATA GGAAAGCAAG TTCCCACCTC AACAAATGCC 
AATCTCAATA ATGCCAATAT GTCCAAAGCT GCCCATGGAA AGCGGCCCAG CATTGGGAAC 
CTTGAGCATG TGTCTGAAAA TGGGCATCAT TCTTCCCACA AGCATGACCG GGAGCCTCAG 
AGAAGGTCCA GTGTGAAAAG AACCCGCTAT TATGAAACTT ACATTAGGTC CGACTCAGGA 
GATGAACAGC TCCCAACTAT TTGCCGGGAA GACCCAGAGA TACATGGCTA TTTCAGGGAC 
CCCCACTGCT TGGGGGAGCA GGAGTATTTC AGTAGTGAGG AATGCTACGA GGATGACAGC 
TCGCCCACCT GGAGCAGGCA AAACTATGGC TACTACAGCA GATACCCAGG CAGAAACATC 
GACTCTGAGA GGCCCCGAGG CTACCATCAT CCCCAAGGAT TCTTGGAGGA CGATGACTCG 
CCCGTTTGCT ATGATTCACG GAGATCTCCA AGGAGACGCC TACTACCTCC CACCCCAGCA 
TCCCACCGGA GATCCTCCTT CAACTTTGAG TGCCTGCGCC GGCAGAGCAG CCAGGAAGAG 
GTCCCGTCGT CTCCCATCTT CCCCCATCGC ACGGCCCTGC CTCTGCATCT AATGCAGCAA 
CAGATCATGG CAGTTGCCGG CCTAGATTCA AGTAAAGCCC AGAAGTACTC ACCGAGTCAC 
TCGACCCGGT CGTGGGCCAC CCCTCCAGCA ACCCCTCCCT ACCGGGACTG GACACCGTGC 
TACACCCCCC TGATCCAAGT GGAGCAGTCA GAGGCCCTGG ACCAGGTGAA CGGCAGCCTG 
CCGTCCCTGC ACCGCAGCTC CTGGTACACA GACGAGCCCG ACATCTCCTA CCGGACTTTC 
ACACCAGCCA GCCTGACTGT CCCCAGCAGC TTCCGGAACA AAAACAGCGA CAAGCAGAGG 
AGTGCGGACA GCTTGGTGGA GGCAGTCCTG ATATCCGAAG GCTTGGGACG CTATGCAAGG 
GACCCAAAAT TTGTGTCAGC AACAAAACAC GAAATCGCTG ATGCCTGTGA CCTCACCATC 
GACGAGATGG AGAGTGCAGC CAGCACCCTG CTTAATGGGA ACGTGCGTCC CCGAGCCAAC 
GGGGATGTGG GCCCCCTCTC ACACCGGCAG GACTATGAGC TACAGGACTT TGGTCCTGGC 
TACAGCGACG AAGAGCCAGA CCCTGGGAGG GATGAGGAGG ACCTGGCGGA TGAAATGATA 
TGCATCACCA CCTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_017007139.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862628.1 Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X4, mRNA.

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CloneID	OHu94541
Clone ID Related Accession (Same CDS sequence)	XM_017007139.1
Accession Version	XM_017007139.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGGG AGCCGCTGCC GCTGTGGGGC TCCCCTCCCC AGCTCTGGCC CGGGCACCAC 
GTGCAGCTTC CACGCCGGCC GGGGCTGGGT CGAGGGAGGA TGGAGCAGCT CCTGGCCAGC 
CCTCCCCTTT GGAGACCGCC AGTGCCCCCC AACCCCTGTC GGCTGGAAGC CTTTCCGGAG 
GCAAACTATG CAAGAGGCAC CAGACTTCCT CTTTCTGGTG AAGGACCAAC TTCTCAGCCG 
AATAGCTCCA AGCAAACTGT CCTGTCTTGG CAAGCTGCAA TCGATGCTGC TAGACAGGCC 
AAGGCTGCCC AAACTATGAG CACCTCTGCA CCCCCACCTG TAGGATCTCT CTCCCAAAGA 
AAACGTCAGC AATACGCCAA GAGCAAAAAA CAGGGTAACT CGTCCAACAG CCGACCTGCC 
CGCGCCCTTT TCTGTTTATC ACTCAATAAC CCCATCCGAA GAGCCTGCAT TAGTATAGTG 
GAATGGAAAC CATTTGACAT ATTTATATTA TTGGCTATTT TTGCCAATTG TGTGGCCTTA 
GCTATTTACA TCCCATTCCC TGAAGATGAT TCTAATTCAA CAAATCATAA CTTGGAAAAA 
GTAGAATATG CCTTCCTGAT TATTTTTACA GTCGAGACAT TTTTGAAGAT TATAGCGTAT 
GGATTATTGC TACATCCTAA TGCTTATGTT AGGAATGGAT GGAATTTACT GGATTTTGTT 
ATAGTAATAG TAGGATTGTT TAGTGTAATT TTGGAACAAT TAACCAAAGA AACAGAAGGC 
GGGAACCACT CAAGCGGCAA ATCTGGAGGC TTTGATGTCA AAGCCCTCCG TGCCTTTCGA 
GTGTTGCGAC CACTTCGACT AGTGTCAGGA GTGCCCAGTT TACAAGTTGT CCTGAACTCC 
ATTATAAAAG CCATGGTTCC CCTCCTTCAC ATAGCCCTTT TGGTATTATT TGTAATCATA 
ATCTATGCTA TTATAGGATT GGAACTTTTT ATTGGAAAAA TGCACAAAAC ATGTTTTTTT 
GCTGACTCAG ATATCGTAGC TGAAGAGGAC CCAGCTCCAT GTGCGTTCTC AGGGAATGGA 
CGCCAGTGTA CTGCCAATGG CACGGAATGT AGGAGTGGCT GGGTTGGCCC GAACGGAGGC 
ATCACCAACT TTGATAACTT TGCCTTTGCC ATGCTTACTG TGTTTCAGTG CATCACCATG 
GAGGGCTGGA CAGATGTGCT CTACTGGGTA AATGATGCGA TAGGATGGGA ATGGCCATGG 
GTGTATTTTG TTAGTCTGAT CATCCTTGGC TCATTTTTCG TCCTTAACCT GGTTCTTGGT 
GTCCTTAGTG GAGAATTCTC AAAGGAAAGA GAGAAGGCAA AAGCACGGGG AGATTTCCAG 
AAGCTCCGGG AGAAGCAGCA GCTGGAGGAG GATCTAAAGG GCTACTTGGA TTGGATCACC 
CAAGCTGAGG ACATCGATCC GGAGAATGAG GAAGAAGGAG GAGAGGAAGG CAAACGAAAT 
ACTAGCATGC CCACCAGCGA GACTGAGTCT GTGAACACAG AGAACGTCAG CGGTGAAGGC 
GAGAACCGAG GCTGCTGTGG AAGTCTCTGG TGCTGGTGGA GACGGAGAGG CGCGGCCAAG 
GCGGGGCCCT CTGGGTGTCG GCGGTGGGGT CAAGCCATCT CAAAATCCAA ACTCAGCCGA 
CGCTGGCGTC GCTGGAACCG ATTCAATCGC AGAAGATGTA GGGCCGCCGT GAAGTCTGTC 
ACGTTTTACT GGCTGGTTAT CGTCCTGGTG TTTCTGAACA CCTTAACCAT TTCCTCTGAG 
CACTACAATC AGCCAGATTG GTTGACACAG ATTCAAGATA TTGCCAACAA AGTCCTCTTG 
GCTCTGTTCA CCTGCGAGAT GCTGGTAAAA ATGTACAGCT TGGGCCTCCA AGCATATTTC 
GTCTCTCTTT TCAACCGGTT TGATTGCTTC GTGGTGTGTG GTGGAATCAC TGAGACGATC 
TTGGTGGAAC TGGAAATCAT GTCTCCCCTG GGGATCTCTG TGTTTCGGTG TGTGCGCCTC 
TTAAGAATCT TCAAAGTGAC CAGGCACTGG ACTTCCCTGA GCAACTTAGT GGCATCCTTA 
TTAAACTCCA TGAAGTCCAT CGCTTCGCTG TTGCTTCTGC TTTTTCTCTT CATTATCATC 
TTTTCCTTGC TTGGGATGCA GCTGTTTGGC GGCAAGTTTA ATTTTGATGA AACGCAAACC 
AAGCGGAGCA CCTTTGACAA TTTCCCTCAA GCACTTCTCA CAGTGTTCCA GATCCTGACA 
GGCGAAGACT GGAATGCTGT GATGTACGAT GGCATCATGG CTTACGGGGG CCCATCCTCT 
TCAGGAATGA TCGTCTGCAT CTACTTCATC ATCCTCTTCA TTTGTGGTAA CTATATTCTA 
CTGAATGTCT TCTTGGCCAT CGCTGTAGAC AATTTGGCTG ATGCTGAAAG TCTGAACACT 
GCTCAGAAAG AAGAAGCGGA AGAAAAGGAG AGGAAAAAGA TTGCCAGAAA AGAGAGCCTA 
GAAAATAAAA AGAACAACAA ACCAGAAGTC AACCAGATAG CCAACAGTGA CAACAAGGTT 
ACAATTGATG ACTATAGAGA AGAGGATGAA GACAAGGACC CCTATCCGCC TTGCGATGTG 
CCAGTAGGGG AAGAGGAAGA GGAAGAGGAG GAGGATGAAC CTGAGGTTCC TGCCGGACCC 
CGTCCTCGAA GGATCTCGGA GTTGAACATG AAGGAAAAAA TTGCCCCCAT CCCTGAAGGG 
AGCGCTTTCT TCATTCTTAG CAAGACCAAC CCGATCCGCG TAGGCTGCCA CAAGCTCATC 
AACCACCACA TCTTCACCAA CCTCATCCTT GTCTTCATCA TGCTGAGCAG CGCTGCCCTG 
GCCGCAGAGG ACCCCATCCG CAGCCACTCC TTCCGGAACA CGATACTGGG TTACTTTGAC 
TATGCCTTCA CAGCCATCTT TACTGTTGAG ATCCTGTTGA AGATGACAAC TTTTGGAGCT 
TTCCTCCACA AAGGGGCCTT CTGCAGGAAC TACTTCAATT TGCTGGATAT GCTGGTGGTT 
GGGGTGTCTC TGGTGTCATT TGGGATTCAA TCCAGTGCCA TCTCCGTTGT GAAGATTCTG 
AGGGTCTTAA GGGTCCTGCG TCCCCTCAGG GCCATCAACA GAGCAAAAGG ACTTAAGCAC 
GTGGTCCAGT GCGTCTTCGT GGCCATCCGG ACCATCGGCA ACATCATGAT CGTCACCACC 
CTCCTGCAGT TCATGTTTGC CTGTATCGGG GTCCAGTTGT TCAAGGGGAA GTTCTATCGC 
TGTACGGATG AAGCCAAAAG TAACCCTGAA GAATGCAGGG GACTTTTCAT CCTCTACAAG 
GATGGGGATG TTGACAGTCC TGTGGTCCGT GAACGGATCT GGCAAAACAG TGATTTCAAC 
TTCGACAACG TCCTCTCTGC TATGATGGCG CTCTTCACAG TCTCCACGTT TGAGGGCTGG 
CCTGCGTTGC TGTATAAAGC CATCGACTCG AATGGAGAGA ACATCGGCCC AATCTACAAC 
CACCGCGTGG AGATCTCCAT CTTCTTCATC ATCTACATCA TCATTGTAGC TTTCTTCATG 
ATGAACATCT TTGTGGGCTT TGTCATCGTT ACATTTCAGG AACAAGGAGA AAAAGAGTAT 
AAGAACTGTG AGCTGGACAA AAATCAGCGT CAGTGTGTTG AATACGCCTT GAAAGCACGT 
CCCTTGCGGA GATACATCCC CAAAAACCCC TACCAGTACA AGTTCTGGTA CGTGGTGAAC 
TCTTCGCCTT TCGAATACAT GATGTTTGTC CTCATCATGC TCAACACACT CTGCTTGGCC 
ATGCAGCACT ACGAGCAGTC CAAGATGTTC AATGATGCCA TGGACATTCT GAACATGGTC 
TTCACCGGGG TGTTCACCGT CGAGATGGTT TTGAAAGTCA TCGCATTTAA GCCTAAGGGG 
TATTTTAGTG ACGCCTGGAA CACGTTTGAC TCCCTCATCG TAATCGGCAG CATTATAGAC 
GTGGCCCTCA GCGAAGCAGA CCACTATTTC ACTGATGCAT GGAACACTTT TGATGCCTTA 
ATTGTTGTTG GTAGCGTCGT TGATATTGCT ATAACTGAAG TGAATCCAAC TGAAAGTGAA 
AATGTCCCTG TCCCAACTGC TACACCTGGG AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGA TTGGTGAAGC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACAT TGCTGTGGAC TTTTATTAAG TCCTTTCAGG CGCTCCCGTA TGTGGCCCTC 
CTCATAGCCA TGCTGTTCTT CATCTATGCG GTCATTGGCA TGCAGATGTT TGGGAAAGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAATAACT TCCAGACGTT TCCCCAGGCG 
GTGCTGCTGC TCTTCAGGTG TGCAACAGGT GAGGCCTGGC AGGAGATCAT GCTGGCCTGT 
CTCCCAGGGA AGCTCTGTGA CCCTGAGTCA GATTACAACC CCGGGGAGGA GTATACATGT 
GGGAGCAACT TTGCCATTGT CTATTTCATC AGTTTTTACA TGCTCTGTGC ATTTCTGATC 
ATCAATCTGT TTGTGGCTGT CATCATGGAT AATTTCGACT ATCTGACCCG GGACTGGTCT 
ATTTTGGGGC CTCACCATTT AGATGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAG 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTCTGC TTCGACGCAT CCAGCCTCCC 
CTGGGGTTTG GGAAGTTATG TCCACACAGG GTAGCGTGCA AGAGATTAGT TGCCATGAAC 
ATGCCTCTCA ACAGTGACGG GACAGTCATG TTTAATGCAA CCCTGTTTGC TTTGGTTCGA 
ACGGCTCTTA AGATCAAGAC CGAAGGGAAC CTGGAGCAAG CTAATGAAGA ACTTCGGGCT 
GTGATAAAGA AAATTTGGAA GAAAACCAGC ATGAAATTAC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAGTACCC TGCGAAGAAC 
ACCACAATTG CCCTACAGGC GGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CGTGTGATTT GCAAGATGAC GAGCCTGAGG AAACAAAACG AGAAGAAGAA 
GATGATGTGT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ATTCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAATTC CACCTGCAAG TGATACTGAG AAACCGCTGT TTCCTCCAGC AGGAAATTCG 
GTGTGTCATA ACCATCATAA CCATAATTCC ATAGGAAAGC AAGTTCCCAC CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTCCAAA GCTGCCCATG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC ATGTGTCTGA AAATGGGCAT CATTCTTCCC ACAAGCATGA CCGGGAGCCT 
CAGAGAAGGT CCAGTGTGAA AAGGTCCGAC TCAGGAGATG AACAGCTCCC AACTATTTGC 
CGGGAAGACC CAGAGATACA TGGCTATTTC AGGGACCCCC ACTGCTTGGG GGAGCAGGAG 
TATTTCAGTA GTGAGGAATG CTACGAGGAT GACAGCTCGC CCACCTGGAG CAGGCAAAAC 
TATGGCTACT ACAGCAGATA CCCAGGCAGA AACATCGACT CTGAGAGGCC CCGAGGCTAC 
CATCATCCCC AAGGATTCTT GGAGGACGAT GACTCGCCCG TTTGCTATGA TTCACGGAGA 
TCTCCAAGGA GACGCCTACT ACCTCCCACC CCAGCATCCC ACCGGAGATC CTCCTTCAAC 
TTTGAGTGCC TGCGCCGGCA GAGCAGCCAG GAAGAGGTCC CGTCGTCTCC CATCTTCCCC 
CATCGCACGG CCCTGCCTCT GCATCTAATG CAGCAACAGA TCATGGCAGT TGCCGGCCTA 
GATTCAAGTA AAGCCCAGAA GTACTCACCG AGTCACTCGA CCCGGTCGTG GGCCACCCCT 
CCAGCAACCC CTCCCTACCG GGACTGGACA CCGTGCTACA CCCCCCTGAT CCAAGTGGAG 
CAGTCAGAGG CCCTGGACCA GGTGAACGGC AGCCTGCCGT CCCTGCACCG CAGCTCCTGG 
TACACAGACG AGCCCGACAT CTCCTACCGG ACTTTCACAC CAGCCAGCCT GACTGTCCCC 
AGCAGCTTCC GGAACAAAAA CAGCGACAAG CAGAGGAGTG CGGACAGCTT GGTGGAGGCA 
GTCCTGATAT CCGAAGGCTT GGGACGCTAT GCAAGGGACC CAAAATTTGT GTCAGCAACA 
AAACACGAAA TCGCTGATGC CTGTGACCTC ACCATCGACG AGATGGAGAG TGCAGCCAGC 
ACCCTGCTTA ATGGGAACGT GCGTCCCCGA GCCAACGGGG ATGTGGGCCC CCTCTCACAC 
CGGCAGGACT ATGAGCTACA GGACTTTGGT CCTGGCTACA GCGACGAAGA GCCAGACCCT 
GGGAGGGATG AGGAGGACCT GGCGGATGAA ATGATATGCA TCACCACCTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862628.1
CDS	16..6729
Translation	MPGEPLPLWGSPPQLWPGHHVQLPRRPGLGRGRMEQLLASPPLWRPPVPPNPCRLEAFPEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGENRGCCGSLWCWWRRRGAAKAGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENIGPIYNHRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPTESENVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEDDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGNLEHVSENGHHSSHKHDREPQRRSSVKRSDSGDEQLPTICREDPEIHGYFRDPHCLGEQEYFSSEECYEDDSSPTWSRQNYGYYSRYPGRNIDSERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPASHRRSSFNFECLRRQSSQEEVPSSPIFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVEQSEALDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGNVRPRANGDVGPLSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017007139.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007139.1

ORF Insert Sequence:

ATGCCCGGGG AGCCGCTGCC GCTGTGGGGC TCCCCTCCCC AGCTCTGGCC CGGGCACCAC 
GTGCAGCTTC CACGCCGGCC GGGGCTGGGT CGAGGGAGGA TGGAGCAGCT CCTGGCCAGC 
CCTCCCCTTT GGAGACCGCC AGTGCCCCCC AACCCCTGTC GGCTGGAAGC CTTTCCGGAG 
GCAAACTATG CAAGAGGCAC CAGACTTCCT CTTTCTGGTG AAGGACCAAC TTCTCAGCCG 
AATAGCTCCA AGCAAACTGT CCTGTCTTGG CAAGCTGCAA TCGATGCTGC TAGACAGGCC 
AAGGCTGCCC AAACTATGAG CACCTCTGCA CCCCCACCTG TAGGATCTCT CTCCCAAAGA 
AAACGTCAGC AATACGCCAA GAGCAAAAAA CAGGGTAACT CGTCCAACAG CCGACCTGCC 
CGCGCCCTTT TCTGTTTATC ACTCAATAAC CCCATCCGAA GAGCCTGCAT TAGTATAGTG 
GAATGGAAAC CATTTGACAT ATTTATATTA TTGGCTATTT TTGCCAATTG TGTGGCCTTA 
GCTATTTACA TCCCATTCCC TGAAGATGAT TCTAATTCAA CAAATCATAA CTTGGAAAAA 
GTAGAATATG CCTTCCTGAT TATTTTTACA GTCGAGACAT TTTTGAAGAT TATAGCGTAT 
GGATTATTGC TACATCCTAA TGCTTATGTT AGGAATGGAT GGAATTTACT GGATTTTGTT 
ATAGTAATAG TAGGATTGTT TAGTGTAATT TTGGAACAAT TAACCAAAGA AACAGAAGGC 
GGGAACCACT CAAGCGGCAA ATCTGGAGGC TTTGATGTCA AAGCCCTCCG TGCCTTTCGA 
GTGTTGCGAC CACTTCGACT AGTGTCAGGA GTGCCCAGTT TACAAGTTGT CCTGAACTCC 
ATTATAAAAG CCATGGTTCC CCTCCTTCAC ATAGCCCTTT TGGTATTATT TGTAATCATA 
ATCTATGCTA TTATAGGATT GGAACTTTTT ATTGGAAAAA TGCACAAAAC ATGTTTTTTT 
GCTGACTCAG ATATCGTAGC TGAAGAGGAC CCAGCTCCAT GTGCGTTCTC AGGGAATGGA 
CGCCAGTGTA CTGCCAATGG CACGGAATGT AGGAGTGGCT GGGTTGGCCC GAACGGAGGC 
ATCACCAACT TTGATAACTT TGCCTTTGCC ATGCTTACTG TGTTTCAGTG CATCACCATG 
GAGGGCTGGA CAGATGTGCT CTACTGGGTA AATGATGCGA TAGGATGGGA ATGGCCATGG 
GTGTATTTTG TTAGTCTGAT CATCCTTGGC TCATTTTTCG TCCTTAACCT GGTTCTTGGT 
GTCCTTAGTG GAGAATTCTC AAAGGAAAGA GAGAAGGCAA AAGCACGGGG AGATTTCCAG 
AAGCTCCGGG AGAAGCAGCA GCTGGAGGAG GATCTAAAGG GCTACTTGGA TTGGATCACC 
CAAGCTGAGG ACATCGATCC GGAGAATGAG GAAGAAGGAG GAGAGGAAGG CAAACGAAAT 
ACTAGCATGC CCACCAGCGA GACTGAGTCT GTGAACACAG AGAACGTCAG CGGTGAAGGC 
GAGAACCGAG GCTGCTGTGG AAGTCTCTGG TGCTGGTGGA GACGGAGAGG CGCGGCCAAG 
GCGGGGCCCT CTGGGTGTCG GCGGTGGGGT CAAGCCATCT CAAAATCCAA ACTCAGCCGA 
CGCTGGCGTC GCTGGAACCG ATTCAATCGC AGAAGATGTA GGGCCGCCGT GAAGTCTGTC 
ACGTTTTACT GGCTGGTTAT CGTCCTGGTG TTTCTGAACA CCTTAACCAT TTCCTCTGAG 
CACTACAATC AGCCAGATTG GTTGACACAG ATTCAAGATA TTGCCAACAA AGTCCTCTTG 
GCTCTGTTCA CCTGCGAGAT GCTGGTAAAA ATGTACAGCT TGGGCCTCCA AGCATATTTC 
GTCTCTCTTT TCAACCGGTT TGATTGCTTC GTGGTGTGTG GTGGAATCAC TGAGACGATC 
TTGGTGGAAC TGGAAATCAT GTCTCCCCTG GGGATCTCTG TGTTTCGGTG TGTGCGCCTC 
TTAAGAATCT TCAAAGTGAC CAGGCACTGG ACTTCCCTGA GCAACTTAGT GGCATCCTTA 
TTAAACTCCA TGAAGTCCAT CGCTTCGCTG TTGCTTCTGC TTTTTCTCTT CATTATCATC 
TTTTCCTTGC TTGGGATGCA GCTGTTTGGC GGCAAGTTTA ATTTTGATGA AACGCAAACC 
AAGCGGAGCA CCTTTGACAA TTTCCCTCAA GCACTTCTCA CAGTGTTCCA GATCCTGACA 
GGCGAAGACT GGAATGCTGT GATGTACGAT GGCATCATGG CTTACGGGGG CCCATCCTCT 
TCAGGAATGA TCGTCTGCAT CTACTTCATC ATCCTCTTCA TTTGTGGTAA CTATATTCTA 
CTGAATGTCT TCTTGGCCAT CGCTGTAGAC AATTTGGCTG ATGCTGAAAG TCTGAACACT 
GCTCAGAAAG AAGAAGCGGA AGAAAAGGAG AGGAAAAAGA TTGCCAGAAA AGAGAGCCTA 
GAAAATAAAA AGAACAACAA ACCAGAAGTC AACCAGATAG CCAACAGTGA CAACAAGGTT 
ACAATTGATG ACTATAGAGA AGAGGATGAA GACAAGGACC CCTATCCGCC TTGCGATGTG 
CCAGTAGGGG AAGAGGAAGA GGAAGAGGAG GAGGATGAAC CTGAGGTTCC TGCCGGACCC 
CGTCCTCGAA GGATCTCGGA GTTGAACATG AAGGAAAAAA TTGCCCCCAT CCCTGAAGGG 
AGCGCTTTCT TCATTCTTAG CAAGACCAAC CCGATCCGCG TAGGCTGCCA CAAGCTCATC 
AACCACCACA TCTTCACCAA CCTCATCCTT GTCTTCATCA TGCTGAGCAG CGCTGCCCTG 
GCCGCAGAGG ACCCCATCCG CAGCCACTCC TTCCGGAACA CGATACTGGG TTACTTTGAC 
TATGCCTTCA CAGCCATCTT TACTGTTGAG ATCCTGTTGA AGATGACAAC TTTTGGAGCT 
TTCCTCCACA AAGGGGCCTT CTGCAGGAAC TACTTCAATT TGCTGGATAT GCTGGTGGTT 
GGGGTGTCTC TGGTGTCATT TGGGATTCAA TCCAGTGCCA TCTCCGTTGT GAAGATTCTG 
AGGGTCTTAA GGGTCCTGCG TCCCCTCAGG GCCATCAACA GAGCAAAAGG ACTTAAGCAC 
GTGGTCCAGT GCGTCTTCGT GGCCATCCGG ACCATCGGCA ACATCATGAT CGTCACCACC 
CTCCTGCAGT TCATGTTTGC CTGTATCGGG GTCCAGTTGT TCAAGGGGAA GTTCTATCGC 
TGTACGGATG AAGCCAAAAG TAACCCTGAA GAATGCAGGG GACTTTTCAT CCTCTACAAG 
GATGGGGATG TTGACAGTCC TGTGGTCCGT GAACGGATCT GGCAAAACAG TGATTTCAAC 
TTCGACAACG TCCTCTCTGC TATGATGGCG CTCTTCACAG TCTCCACGTT TGAGGGCTGG 
CCTGCGTTGC TGTATAAAGC CATCGACTCG AATGGAGAGA ACATCGGCCC AATCTACAAC 
CACCGCGTGG AGATCTCCAT CTTCTTCATC ATCTACATCA TCATTGTAGC TTTCTTCATG 
ATGAACATCT TTGTGGGCTT TGTCATCGTT ACATTTCAGG AACAAGGAGA AAAAGAGTAT 
AAGAACTGTG AGCTGGACAA AAATCAGCGT CAGTGTGTTG AATACGCCTT GAAAGCACGT 
CCCTTGCGGA GATACATCCC CAAAAACCCC TACCAGTACA AGTTCTGGTA CGTGGTGAAC 
TCTTCGCCTT TCGAATACAT GATGTTTGTC CTCATCATGC TCAACACACT CTGCTTGGCC 
ATGCAGCACT ACGAGCAGTC CAAGATGTTC AATGATGCCA TGGACATTCT GAACATGGTC 
TTCACCGGGG TGTTCACCGT CGAGATGGTT TTGAAAGTCA TCGCATTTAA GCCTAAGGGG 
TATTTTAGTG ACGCCTGGAA CACGTTTGAC TCCCTCATCG TAATCGGCAG CATTATAGAC 
GTGGCCCTCA GCGAAGCAGA CCACTATTTC ACTGATGCAT GGAACACTTT TGATGCCTTA 
ATTGTTGTTG GTAGCGTCGT TGATATTGCT ATAACTGAAG TGAATCCAAC TGAAAGTGAA 
AATGTCCCTG TCCCAACTGC TACACCTGGG AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGA TTGGTGAAGC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACAT TGCTGTGGAC TTTTATTAAG TCCTTTCAGG CGCTCCCGTA TGTGGCCCTC 
CTCATAGCCA TGCTGTTCTT CATCTATGCG GTCATTGGCA TGCAGATGTT TGGGAAAGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAATAACT TCCAGACGTT TCCCCAGGCG 
GTGCTGCTGC TCTTCAGGTG TGCAACAGGT GAGGCCTGGC AGGAGATCAT GCTGGCCTGT 
CTCCCAGGGA AGCTCTGTGA CCCTGAGTCA GATTACAACC CCGGGGAGGA GTATACATGT 
GGGAGCAACT TTGCCATTGT CTATTTCATC AGTTTTTACA TGCTCTGTGC ATTTCTGATC 
ATCAATCTGT TTGTGGCTGT CATCATGGAT AATTTCGACT ATCTGACCCG GGACTGGTCT 
ATTTTGGGGC CTCACCATTT AGATGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAG 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTCTGC TTCGACGCAT CCAGCCTCCC 
CTGGGGTTTG GGAAGTTATG TCCACACAGG GTAGCGTGCA AGAGATTAGT TGCCATGAAC 
ATGCCTCTCA ACAGTGACGG GACAGTCATG TTTAATGCAA CCCTGTTTGC TTTGGTTCGA 
ACGGCTCTTA AGATCAAGAC CGAAGGGAAC CTGGAGCAAG CTAATGAAGA ACTTCGGGCT 
GTGATAAAGA AAATTTGGAA GAAAACCAGC ATGAAATTAC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAGTACCC TGCGAAGAAC 
ACCACAATTG CCCTACAGGC GGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CGTGTGATTT GCAAGATGAC GAGCCTGAGG AAACAAAACG AGAAGAAGAA 
GATGATGTGT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ATTCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAATTC CACCTGCAAG TGATACTGAG AAACCGCTGT TTCCTCCAGC AGGAAATTCG 
GTGTGTCATA ACCATCATAA CCATAATTCC ATAGGAAAGC AAGTTCCCAC CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTCCAAA GCTGCCCATG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC ATGTGTCTGA AAATGGGCAT CATTCTTCCC ACAAGCATGA CCGGGAGCCT 
CAGAGAAGGT CCAGTGTGAA AAGGTCCGAC TCAGGAGATG AACAGCTCCC AACTATTTGC 
CGGGAAGACC CAGAGATACA TGGCTATTTC AGGGACCCCC ACTGCTTGGG GGAGCAGGAG 
TATTTCAGTA GTGAGGAATG CTACGAGGAT GACAGCTCGC CCACCTGGAG CAGGCAAAAC 
TATGGCTACT ACAGCAGATA CCCAGGCAGA AACATCGACT CTGAGAGGCC CCGAGGCTAC 
CATCATCCCC AAGGATTCTT GGAGGACGAT GACTCGCCCG TTTGCTATGA TTCACGGAGA 
TCTCCAAGGA GACGCCTACT ACCTCCCACC CCAGCATCCC ACCGGAGATC CTCCTTCAAC 
TTTGAGTGCC TGCGCCGGCA GAGCAGCCAG GAAGAGGTCC CGTCGTCTCC CATCTTCCCC 
CATCGCACGG CCCTGCCTCT GCATCTAATG CAGCAACAGA TCATGGCAGT TGCCGGCCTA 
GATTCAAGTA AAGCCCAGAA GTACTCACCG AGTCACTCGA CCCGGTCGTG GGCCACCCCT 
CCAGCAACCC CTCCCTACCG GGACTGGACA CCGTGCTACA CCCCCCTGAT CCAAGTGGAG 
CAGTCAGAGG CCCTGGACCA GGTGAACGGC AGCCTGCCGT CCCTGCACCG CAGCTCCTGG 
TACACAGACG AGCCCGACAT CTCCTACCGG ACTTTCACAC CAGCCAGCCT GACTGTCCCC 
AGCAGCTTCC GGAACAAAAA CAGCGACAAG CAGAGGAGTG CGGACAGCTT GGTGGAGGCA 
GTCCTGATAT CCGAAGGCTT GGGACGCTAT GCAAGGGACC CAAAATTTGT GTCAGCAACA 
AAACACGAAA TCGCTGATGC CTGTGACCTC ACCATCGACG AGATGGAGAG TGCAGCCAGC 
ACCCTGCTTA ATGGGAACGT GCGTCCCCGA GCCAACGGGG ATGTGGGCCC CCTCTCACAC 
CGGCAGGACT ATGAGCTACA GGACTTTGGT CCTGGCTACA GCGACGAAGA GCCAGACCCT 
GGGAGGGATG AGGAGGACCT GGCGGATGAA ATGATATGCA TCACCACCTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_017007140.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862629.1 Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X5, mRNA.

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CloneID	OHu94542
Clone ID Related Accession (Same CDS sequence)	XM_017007140.1
Accession Version	XM_017007140.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGGG AGCCGCTGCC GCTGTGGGGC TCCCCTCCCC AGCTCTGGCC CGGGCACCAC 
GTGCAGCTTC CACGCCGGCC GGGGCTGGGT CGAGGGAGGA TGGAGCAGCT CCTGGCCAGC 
CCTCCCCTTT GGAGACCGCC AGTGCCCCCC AACCCCTGTC GGCTGGAAGC CTTTCCGGAG 
GCAAACTATG CAAGAGGCAC CAGACTTCCT CTTTCTGGTG AAGGACCAAC TTCTCAGCCG 
AATAGCTCCA AGCAAACTGT CCTGTCTTGG CAAGCTGCAA TCGATGCTGC TAGACAGGCC 
AAGGCTGCCC AAACTATGAG CACCTCTGCA CCCCCACCTG TAGGATCTCT CTCCCAAAGA 
AAACGTCAGC AATACGCCAA GAGCAAAAAA CAGGGTAACT CGTCCAACAG CCGACCTGCC 
CGCGCCCTTT TCTGTTTATC ACTCAATAAC CCCATCCGAA GAGCCTGCAT TAGTATAGTG 
GAATGGAAAC CATTTGACAT ATTTATATTA TTGGCTATTT TTGCCAATTG TGTGGCCTTA 
GCTATTTACA TCCCATTCCC TGAAGATGAT TCTAATTCAA CAAATCATAA CTTGGAAAAA 
GTAGAATATG CCTTCCTGAT TATTTTTACA GTCGAGACAT TTTTGAAGAT TATAGCGTAT 
GGATTATTGC TACATCCTAA TGCTTATGTT AGGAATGGAT GGAATTTACT GGATTTTGTT 
ATAGTAATAG TAGGATTGTT TAGTGTAATT TTGGAACAAT TAACCAAAGA AACAGAAGGC 
GGGAACCACT CAAGCGGCAA ATCTGGAGGC TTTGATGTCA AAGCCCTCCG TGCCTTTCGA 
GTGTTGCGAC CACTTCGACT AGTGTCAGGA GTGCCCAGTT TACAAGTTGT CCTGAACTCC 
ATTATAAAAG CCATGGTTCC CCTCCTTCAC ATAGCCCTTT TGGTATTATT TGTAATCATA 
ATCTATGCTA TTATAGGATT GGAACTTTTT ATTGGAAAAA TGCACAAAAC ATGTTTTTTT 
GCTGACTCAG ATATCGTAGC TGAAGAGGAC CCAGCTCCAT GTGCGTTCTC AGGGAATGGA 
CGCCAGTGTA CTGCCAATGG CACGGAATGT AGGAGTGGCT GGGTTGGCCC GAACGGAGGC 
ATCACCAACT TTGATAACTT TGCCTTTGCC ATGCTTACTG TGTTTCAGTG CATCACCATG 
GAGGGCTGGA CAGATGTGCT CTACTGGGTA AATGATGCGA TAGGATGGGA ATGGCCATGG 
GTGTATTTTG TTAGTCTGAT CATCCTTGGC TCATTTTTCG TCCTTAACCT GGTTCTTGGT 
GTCCTTAGTG GAGAATTCTC AAAGGAAAGA GAGAAGGCAA AAGCACGGGG AGATTTCCAG 
AAGCTCCGGG AGAAGCAGCA GCTGGAGGAG GATCTAAAGG GCTACTTGGA TTGGATCACC 
CAAGCTGAGG ACATCGATCC GGAGAATGAG GAAGAAGGAG GAGAGGAAGG CAAACGAAAT 
ACTAGCATGC CCACCAGCGA GACTGAGTCT GTGAACACAG AGAACGTCAG CGGTGAAGGC 
GAGAACCGAG GCTGCTGTGG AAGTCTCTGT CAAGCCATCT CAAAATCCAA ACTCAGCCGA 
CGCTGGCGTC GCTGGAACCG ATTCAATCGC AGAAGATGTA GGGCCGCCGT GAAGTCTGTC 
ACGTTTTACT GGCTGGTTAT CGTCCTGGTG TTTCTGAACA CCTTAACCAT TTCCTCTGAG 
CACTACAATC AGCCAGATTG GTTGACACAG ATTCAAGATA TTGCCAACAA AGTCCTCTTG 
GCTCTGTTCA CCTGCGAGAT GCTGGTAAAA ATGTACAGCT TGGGCCTCCA AGCATATTTC 
GTCTCTCTTT TCAACCGGTT TGATTGCTTC GTGGTGTGTG GTGGAATCAC TGAGACGATC 
TTGGTGGAAC TGGAAATCAT GTCTCCCCTG GGGATCTCTG TGTTTCGGTG TGTGCGCCTC 
TTAAGAATCT TCAAAGTGAC CAGGCACTGG ACTTCCCTGA GCAACTTAGT GGCATCCTTA 
TTAAACTCCA TGAAGTCCAT CGCTTCGCTG TTGCTTCTGC TTTTTCTCTT CATTATCATC 
TTTTCCTTGC TTGGGATGCA GCTGTTTGGC GGCAAGTTTA ATTTTGATGA AACGCAAACC 
AAGCGGAGCA CCTTTGACAA TTTCCCTCAA GCACTTCTCA CAGTGTTCCA GATCCTGACA 
GGCGAAGACT GGAATGCTGT GATGTACGAT GGCATCATGG CTTACGGGGG CCCATCCTCT 
TCAGGAATGA TCGTCTGCAT CTACTTCATC ATCCTCTTCA TTTGTGGTAA CTATATTCTA 
CTGAATGTCT TCTTGGCCAT CGCTGTAGAC AATTTGGCTG ATGCTGAAAG TCTGAACACT 
GCTCAGAAAG AAGAAGCGGA AGAAAAGGAG AGGAAAAAGA TTGCCAGAAA AGAGAGCCTA 
GAAAATAAAA AGAACAACAA ACCAGAAGTC AACCAGATAG CCAACAGTGA CAACAAGGTT 
ACAATTGATG ACTATAGAGA AGAGGATGAA GACAAGGACC CCTATCCGCC TTGCGATGTG 
CCAGTAGGGG AAGAGGAAGA GGAAGAGGAG GAGGATGAAC CTGAGGTTCC TGCCGGACCC 
CGTCCTCGAA GGATCTCGGA GTTGAACATG AAGGAAAAAA TTGCCCCCAT CCCTGAAGGG 
AGCGCTTTCT TCATTCTTAG CAAGACCAAC CCGATCCGCG TAGGCTGCCA CAAGCTCATC 
AACCACCACA TCTTCACCAA CCTCATCCTT GTCTTCATCA TGCTGAGCAG CGCTGCCCTG 
GCCGCAGAGG ACCCCATCCG CAGCCACTCC TTCCGGAACA CGATACTGGG TTACTTTGAC 
TATGCCTTCA CAGCCATCTT TACTGTTGAG ATCCTGTTGA AGATGACAAC TTTTGGAGCT 
TTCCTCCACA AAGGGGCCTT CTGCAGGAAC TACTTCAATT TGCTGGATAT GCTGGTGGTT 
GGGGTGTCTC TGGTGTCATT TGGGATTCAA TCCAGTGCCA TCTCCGTTGT GAAGATTCTG 
AGGGTCTTAA GGGTCCTGCG TCCCCTCAGG GCCATCAACA GAGCAAAAGG ACTTAAGCAC 
GTGGTCCAGT GCGTCTTCGT GGCCATCCGG ACCATCGGCA ACATCATGAT CGTCACCACC 
CTCCTGCAGT TCATGTTTGC CTGTATCGGG GTCCAGTTGT TCAAGGGGAA GTTCTATCGC 
TGTACGGATG AAGCCAAAAG TAACCCTGAA GAATGCAGGG GACTTTTCAT CCTCTACAAG 
GATGGGGATG TTGACAGTCC TGTGGTCCGT GAACGGATCT GGCAAAACAG TGATTTCAAC 
TTCGACAACG TCCTCTCTGC TATGATGGCG CTCTTCACAG TCTCCACGTT TGAGGGCTGG 
CCTGCGTTGC TGTATAAAGC CATCGACTCG AATGGAGAGA ACATCGGCCC AATCTACAAC 
CACCGCGTGG AGATCTCCAT CTTCTTCATC ATCTACATCA TCATTGTAGC TTTCTTCATG 
ATGAACATCT TTGTGGGCTT TGTCATCGTT ACATTTCAGG AACAAGGAGA AAAAGAGTAT 
AAGAACTGTG AGCTGGACAA AAATCAGCGT CAGTGTGTTG AATACGCCTT GAAAGCACGT 
CCCTTGCGGA GATACATCCC CAAAAACCCC TACCAGTACA AGTTCTGGTA CGTGGTGAAC 
TCTTCGCCTT TCGAATACAT GATGTTTGTC CTCATCATGC TCAACACACT CTGCTTGGCC 
ATGCAGCACT ACGAGCAGTC CAAGATGTTC AATGATGCCA TGGACATTCT GAACATGGTC 
TTCACCGGGG TGTTCACCGT CGAGATGGTT TTGAAAGTCA TCGCATTTAA GCCTAAGGGG 
TATTTTAGTG ACGCCTGGAA CACGTTTGAC TCCCTCATCG TAATCGGCAG CATTATAGAC 
GTGGCCCTCA GCGAAGCAGA CCACTATTTC ACTGATGCAT GGAACACTTT TGATGCCTTA 
ATTGTTGTTG GTAGCGTCGT TGATATTGCT ATAACTGAAG TGAATCCAAC TGAAAGTGAA 
AATGTCCCTG TCCCAACTGC TACACCTGGG AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGA TTGGTGAAGC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACAT TGCTGTGGAC TTTTATTAAG TCCTTTCAGG CGCTCCCGTA TGTGGCCCTC 
CTCATAGCCA TGCTGTTCTT CATCTATGCG GTCATTGGCA TGCAGATGTT TGGGAAAGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAATAACT TCCAGACGTT TCCCCAGGCG 
GTGCTGCTGC TCTTCAGGTG TGCAACAGGT GAGGCCTGGC AGGAGATCAT GCTGGCCTGT 
CTCCCAGGGA AGCTCTGTGA CCCTGAGTCA GATTACAACC CCGGGGAGGA GTATACATGT 
GGGAGCAACT TTGCCATTGT CTATTTCATC AGTTTTTACA TGCTCTGTGC ATTTCTGATC 
ATCAATCTGT TTGTGGCTGT CATCATGGAT AATTTCGACT ATCTGACCCG GGACTGGTCT 
ATTTTGGGGC CTCACCATTT AGATGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAG 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTCTGC TTCGACGCAT CCAGCCTCCC 
CTGGGGTTTG GGAAGTTATG TCCACACAGG GTAGCGTGCA AGAGATTAGT TGCCATGAAC 
ATGCCTCTCA ACAGTGACGG GACAGTCATG TTTAATGCAA CCCTGTTTGC TTTGGTTCGA 
ACGGCTCTTA AGATCAAGAC CGAAGGGAAC CTGGAGCAAG CTAATGAAGA ACTTCGGGCT 
GTGATAAAGA AAATTTGGAA GAAAACCAGC ATGAAATTAC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAGTACCC TGCGAAGAAC 
ACCACAATTG CCCTACAGGC GGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CGTGTGATTT GCAAGATGAC GAGCCTGAGG AAACAAAACG AGAAGAAGAA 
GATGATGTGT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ATTCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAATTC CACCTGCAAG TGATACTGAG AAACCGCTGT TTCCTCCAGC AGGAAATTCG 
GTGTGTCATA ACCATCATAA CCATAATTCC ATAGGAAAGC AAGTTCCCAC CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTCCAAA GCTGCCCATG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC ATGTGTCTGA AAATGGGCAT CATTCTTCCC ACAAGCATGA CCGGGAGCCT 
CAGAGAAGGT CCAGTGTGAA AAGAACCCGC TATTATGAAA CTTACATTAG GTCCGACTCA 
GGAGATGAAC AGCTCCCAAC TATTTGCCGG GAAGACCCAG AGATACATGG CTATTTCAGG 
GACCCCCACT GCTTGGGGGA GCAGGAGTAT TTCAGTAGTG AGGAATGCTA CGAGGATGAC 
AGCTCGCCCA CCTGGAGCAG GCAAAACTAT GGCTACTACA GCAGATACCC AGGCAGAAAC 
ATCGACTCTG AGAGGCCCCG AGGCTACCAT CATCCCCAAG GATTCTTGGA GGACGATGAC 
TCGCCCGTTT GCTATGATTC ACGGAGATCT CCAAGGAGAC GCCTACTACC TCCCACCCCA 
GCATCCCACC GGAGATCCTC CTTCAACTTT GAGTGCCTGC GCCGGCAGAG CAGCCAGGAA 
GAGGTCCCGT CGTCTCCCAT CTTCCCCCAT CGCACGGCCC TGCCTCTGCA TCTAATGCAG 
CAACAGATCA TGGCAGTTGC CGGCCTAGAT TCAAGTAAAG CCCAGAAGTA CTCACCGAGT 
CACTCGACCC GGTCGTGGGC CACCCCTCCA GCAACCCCTC CCTACCGGGA CTGGACACCG 
TGCTACACCC CCCTGATCCA AGTGGAGCAG TCAGAGGCCC TGGACCAGGT GAACGGCAGC 
CTGCCGTCCC TGCACCGCAG CTCCTGGTAC ACAGACGAGC CCGACATCTC CTACCGGACT 
TTCACACCAG CCAGCCTGAC TGTCCCCAGC AGCTTCCGGA ACAAAAACAG CGACAAGCAG 
AGGAGTGCGG ACAGCTTGGT GGAGGCAGTC CTGATATCCG AAGGCTTGGG ACGCTATGCA 
AGGGACCCAA AATTTGTGTC AGCAACAAAA CACGAAATCG CTGATGCCTG TGACCTCACC 
ATCGACGAGA TGGAGAGTGC AGCCAGCACC CTGCTTAATG GGAACGTGCG TCCCCGAGCC 
AACGGGGATG TGGGCCCCCT CTCACACCGG CAGGACTATG AGCTACAGGA CTTTGGTCCT 
GGCTACAGCG ACGAAGAGCC AGACCCTGGG AGGGATGAGG AGGACCTGGC GGATGAAATG 
ATATGCATCA CCACCTTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862629.1
CDS	16..6696
Translation	MPGEPLPLWGSPPQLWPGHHVQLPRRPGLGRGRMEQLLASPPLWRPPVPPNPCRLEAFPEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGENRGCCGSLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENIGPIYNHRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPTESENVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEDDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGNLEHVSENGHHSSHKHDREPQRRSSVKRTRYYETYIRSDSGDEQLPTICREDPEIHGYFRDPHCLGEQEYFSSEECYEDDSSPTWSRQNYGYYSRYPGRNIDSERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPASHRRSSFNFECLRRQSSQEEVPSSPIFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVEQSEALDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGNVRPRANGDVGPLSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017007140.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007140.1

ORF Insert Sequence:

ATGCCCGGGG AGCCGCTGCC GCTGTGGGGC TCCCCTCCCC AGCTCTGGCC CGGGCACCAC 
GTGCAGCTTC CACGCCGGCC GGGGCTGGGT CGAGGGAGGA TGGAGCAGCT CCTGGCCAGC 
CCTCCCCTTT GGAGACCGCC AGTGCCCCCC AACCCCTGTC GGCTGGAAGC CTTTCCGGAG 
GCAAACTATG CAAGAGGCAC CAGACTTCCT CTTTCTGGTG AAGGACCAAC TTCTCAGCCG 
AATAGCTCCA AGCAAACTGT CCTGTCTTGG CAAGCTGCAA TCGATGCTGC TAGACAGGCC 
AAGGCTGCCC AAACTATGAG CACCTCTGCA CCCCCACCTG TAGGATCTCT CTCCCAAAGA 
AAACGTCAGC AATACGCCAA GAGCAAAAAA CAGGGTAACT CGTCCAACAG CCGACCTGCC 
CGCGCCCTTT TCTGTTTATC ACTCAATAAC CCCATCCGAA GAGCCTGCAT TAGTATAGTG 
GAATGGAAAC CATTTGACAT ATTTATATTA TTGGCTATTT TTGCCAATTG TGTGGCCTTA 
GCTATTTACA TCCCATTCCC TGAAGATGAT TCTAATTCAA CAAATCATAA CTTGGAAAAA 
GTAGAATATG CCTTCCTGAT TATTTTTACA GTCGAGACAT TTTTGAAGAT TATAGCGTAT 
GGATTATTGC TACATCCTAA TGCTTATGTT AGGAATGGAT GGAATTTACT GGATTTTGTT 
ATAGTAATAG TAGGATTGTT TAGTGTAATT TTGGAACAAT TAACCAAAGA AACAGAAGGC 
GGGAACCACT CAAGCGGCAA ATCTGGAGGC TTTGATGTCA AAGCCCTCCG TGCCTTTCGA 
GTGTTGCGAC CACTTCGACT AGTGTCAGGA GTGCCCAGTT TACAAGTTGT CCTGAACTCC 
ATTATAAAAG CCATGGTTCC CCTCCTTCAC ATAGCCCTTT TGGTATTATT TGTAATCATA 
ATCTATGCTA TTATAGGATT GGAACTTTTT ATTGGAAAAA TGCACAAAAC ATGTTTTTTT 
GCTGACTCAG ATATCGTAGC TGAAGAGGAC CCAGCTCCAT GTGCGTTCTC AGGGAATGGA 
CGCCAGTGTA CTGCCAATGG CACGGAATGT AGGAGTGGCT GGGTTGGCCC GAACGGAGGC 
ATCACCAACT TTGATAACTT TGCCTTTGCC ATGCTTACTG TGTTTCAGTG CATCACCATG 
GAGGGCTGGA CAGATGTGCT CTACTGGGTA AATGATGCGA TAGGATGGGA ATGGCCATGG 
GTGTATTTTG TTAGTCTGAT CATCCTTGGC TCATTTTTCG TCCTTAACCT GGTTCTTGGT 
GTCCTTAGTG GAGAATTCTC AAAGGAAAGA GAGAAGGCAA AAGCACGGGG AGATTTCCAG 
AAGCTCCGGG AGAAGCAGCA GCTGGAGGAG GATCTAAAGG GCTACTTGGA TTGGATCACC 
CAAGCTGAGG ACATCGATCC GGAGAATGAG GAAGAAGGAG GAGAGGAAGG CAAACGAAAT 
ACTAGCATGC CCACCAGCGA GACTGAGTCT GTGAACACAG AGAACGTCAG CGGTGAAGGC 
GAGAACCGAG GCTGCTGTGG AAGTCTCTGT CAAGCCATCT CAAAATCCAA ACTCAGCCGA 
CGCTGGCGTC GCTGGAACCG ATTCAATCGC AGAAGATGTA GGGCCGCCGT GAAGTCTGTC 
ACGTTTTACT GGCTGGTTAT CGTCCTGGTG TTTCTGAACA CCTTAACCAT TTCCTCTGAG 
CACTACAATC AGCCAGATTG GTTGACACAG ATTCAAGATA TTGCCAACAA AGTCCTCTTG 
GCTCTGTTCA CCTGCGAGAT GCTGGTAAAA ATGTACAGCT TGGGCCTCCA AGCATATTTC 
GTCTCTCTTT TCAACCGGTT TGATTGCTTC GTGGTGTGTG GTGGAATCAC TGAGACGATC 
TTGGTGGAAC TGGAAATCAT GTCTCCCCTG GGGATCTCTG TGTTTCGGTG TGTGCGCCTC 
TTAAGAATCT TCAAAGTGAC CAGGCACTGG ACTTCCCTGA GCAACTTAGT GGCATCCTTA 
TTAAACTCCA TGAAGTCCAT CGCTTCGCTG TTGCTTCTGC TTTTTCTCTT CATTATCATC 
TTTTCCTTGC TTGGGATGCA GCTGTTTGGC GGCAAGTTTA ATTTTGATGA AACGCAAACC 
AAGCGGAGCA CCTTTGACAA TTTCCCTCAA GCACTTCTCA CAGTGTTCCA GATCCTGACA 
GGCGAAGACT GGAATGCTGT GATGTACGAT GGCATCATGG CTTACGGGGG CCCATCCTCT 
TCAGGAATGA TCGTCTGCAT CTACTTCATC ATCCTCTTCA TTTGTGGTAA CTATATTCTA 
CTGAATGTCT TCTTGGCCAT CGCTGTAGAC AATTTGGCTG ATGCTGAAAG TCTGAACACT 
GCTCAGAAAG AAGAAGCGGA AGAAAAGGAG AGGAAAAAGA TTGCCAGAAA AGAGAGCCTA 
GAAAATAAAA AGAACAACAA ACCAGAAGTC AACCAGATAG CCAACAGTGA CAACAAGGTT 
ACAATTGATG ACTATAGAGA AGAGGATGAA GACAAGGACC CCTATCCGCC TTGCGATGTG 
CCAGTAGGGG AAGAGGAAGA GGAAGAGGAG GAGGATGAAC CTGAGGTTCC TGCCGGACCC 
CGTCCTCGAA GGATCTCGGA GTTGAACATG AAGGAAAAAA TTGCCCCCAT CCCTGAAGGG 
AGCGCTTTCT TCATTCTTAG CAAGACCAAC CCGATCCGCG TAGGCTGCCA CAAGCTCATC 
AACCACCACA TCTTCACCAA CCTCATCCTT GTCTTCATCA TGCTGAGCAG CGCTGCCCTG 
GCCGCAGAGG ACCCCATCCG CAGCCACTCC TTCCGGAACA CGATACTGGG TTACTTTGAC 
TATGCCTTCA CAGCCATCTT TACTGTTGAG ATCCTGTTGA AGATGACAAC TTTTGGAGCT 
TTCCTCCACA AAGGGGCCTT CTGCAGGAAC TACTTCAATT TGCTGGATAT GCTGGTGGTT 
GGGGTGTCTC TGGTGTCATT TGGGATTCAA TCCAGTGCCA TCTCCGTTGT GAAGATTCTG 
AGGGTCTTAA GGGTCCTGCG TCCCCTCAGG GCCATCAACA GAGCAAAAGG ACTTAAGCAC 
GTGGTCCAGT GCGTCTTCGT GGCCATCCGG ACCATCGGCA ACATCATGAT CGTCACCACC 
CTCCTGCAGT TCATGTTTGC CTGTATCGGG GTCCAGTTGT TCAAGGGGAA GTTCTATCGC 
TGTACGGATG AAGCCAAAAG TAACCCTGAA GAATGCAGGG GACTTTTCAT CCTCTACAAG 
GATGGGGATG TTGACAGTCC TGTGGTCCGT GAACGGATCT GGCAAAACAG TGATTTCAAC 
TTCGACAACG TCCTCTCTGC TATGATGGCG CTCTTCACAG TCTCCACGTT TGAGGGCTGG 
CCTGCGTTGC TGTATAAAGC CATCGACTCG AATGGAGAGA ACATCGGCCC AATCTACAAC 
CACCGCGTGG AGATCTCCAT CTTCTTCATC ATCTACATCA TCATTGTAGC TTTCTTCATG 
ATGAACATCT TTGTGGGCTT TGTCATCGTT ACATTTCAGG AACAAGGAGA AAAAGAGTAT 
AAGAACTGTG AGCTGGACAA AAATCAGCGT CAGTGTGTTG AATACGCCTT GAAAGCACGT 
CCCTTGCGGA GATACATCCC CAAAAACCCC TACCAGTACA AGTTCTGGTA CGTGGTGAAC 
TCTTCGCCTT TCGAATACAT GATGTTTGTC CTCATCATGC TCAACACACT CTGCTTGGCC 
ATGCAGCACT ACGAGCAGTC CAAGATGTTC AATGATGCCA TGGACATTCT GAACATGGTC 
TTCACCGGGG TGTTCACCGT CGAGATGGTT TTGAAAGTCA TCGCATTTAA GCCTAAGGGG 
TATTTTAGTG ACGCCTGGAA CACGTTTGAC TCCCTCATCG TAATCGGCAG CATTATAGAC 
GTGGCCCTCA GCGAAGCAGA CCACTATTTC ACTGATGCAT GGAACACTTT TGATGCCTTA 
ATTGTTGTTG GTAGCGTCGT TGATATTGCT ATAACTGAAG TGAATCCAAC TGAAAGTGAA 
AATGTCCCTG TCCCAACTGC TACACCTGGG AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGA TTGGTGAAGC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACAT TGCTGTGGAC TTTTATTAAG TCCTTTCAGG CGCTCCCGTA TGTGGCCCTC 
CTCATAGCCA TGCTGTTCTT CATCTATGCG GTCATTGGCA TGCAGATGTT TGGGAAAGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAATAACT TCCAGACGTT TCCCCAGGCG 
GTGCTGCTGC TCTTCAGGTG TGCAACAGGT GAGGCCTGGC AGGAGATCAT GCTGGCCTGT 
CTCCCAGGGA AGCTCTGTGA CCCTGAGTCA GATTACAACC CCGGGGAGGA GTATACATGT 
GGGAGCAACT TTGCCATTGT CTATTTCATC AGTTTTTACA TGCTCTGTGC ATTTCTGATC 
ATCAATCTGT TTGTGGCTGT CATCATGGAT AATTTCGACT ATCTGACCCG GGACTGGTCT 
ATTTTGGGGC CTCACCATTT AGATGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAG 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTCTGC TTCGACGCAT CCAGCCTCCC 
CTGGGGTTTG GGAAGTTATG TCCACACAGG GTAGCGTGCA AGAGATTAGT TGCCATGAAC 
ATGCCTCTCA ACAGTGACGG GACAGTCATG TTTAATGCAA CCCTGTTTGC TTTGGTTCGA 
ACGGCTCTTA AGATCAAGAC CGAAGGGAAC CTGGAGCAAG CTAATGAAGA ACTTCGGGCT 
GTGATAAAGA AAATTTGGAA GAAAACCAGC ATGAAATTAC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAGTACCC TGCGAAGAAC 
ACCACAATTG CCCTACAGGC GGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CGTGTGATTT GCAAGATGAC GAGCCTGAGG AAACAAAACG AGAAGAAGAA 
GATGATGTGT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ATTCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAATTC CACCTGCAAG TGATACTGAG AAACCGCTGT TTCCTCCAGC AGGAAATTCG 
GTGTGTCATA ACCATCATAA CCATAATTCC ATAGGAAAGC AAGTTCCCAC CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTCCAAA GCTGCCCATG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC ATGTGTCTGA AAATGGGCAT CATTCTTCCC ACAAGCATGA CCGGGAGCCT 
CAGAGAAGGT CCAGTGTGAA AAGAACCCGC TATTATGAAA CTTACATTAG GTCCGACTCA 
GGAGATGAAC AGCTCCCAAC TATTTGCCGG GAAGACCCAG AGATACATGG CTATTTCAGG 
GACCCCCACT GCTTGGGGGA GCAGGAGTAT TTCAGTAGTG AGGAATGCTA CGAGGATGAC 
AGCTCGCCCA CCTGGAGCAG GCAAAACTAT GGCTACTACA GCAGATACCC AGGCAGAAAC 
ATCGACTCTG AGAGGCCCCG AGGCTACCAT CATCCCCAAG GATTCTTGGA GGACGATGAC 
TCGCCCGTTT GCTATGATTC ACGGAGATCT CCAAGGAGAC GCCTACTACC TCCCACCCCA 
GCATCCCACC GGAGATCCTC CTTCAACTTT GAGTGCCTGC GCCGGCAGAG CAGCCAGGAA 
GAGGTCCCGT CGTCTCCCAT CTTCCCCCAT CGCACGGCCC TGCCTCTGCA TCTAATGCAG 
CAACAGATCA TGGCAGTTGC CGGCCTAGAT TCAAGTAAAG CCCAGAAGTA CTCACCGAGT 
CACTCGACCC GGTCGTGGGC CACCCCTCCA GCAACCCCTC CCTACCGGGA CTGGACACCG 
TGCTACACCC CCCTGATCCA AGTGGAGCAG TCAGAGGCCC TGGACCAGGT GAACGGCAGC 
CTGCCGTCCC TGCACCGCAG CTCCTGGTAC ACAGACGAGC CCGACATCTC CTACCGGACT 
TTCACACCAG CCAGCCTGAC TGTCCCCAGC AGCTTCCGGA ACAAAAACAG CGACAAGCAG 
AGGAGTGCGG ACAGCTTGGT GGAGGCAGTC CTGATATCCG AAGGCTTGGG ACGCTATGCA 
AGGGACCCAA AATTTGTGTC AGCAACAAAA CACGAAATCG CTGATGCCTG TGACCTCACC 
ATCGACGAGA TGGAGAGTGC AGCCAGCACC CTGCTTAATG GGAACGTGCG TCCCCGAGCC 
AACGGGGATG TGGGCCCCCT CTCACACCGG CAGGACTATG AGCTACAGGA CTTTGGTCCT 
GGCTACAGCG ACGAAGAGCC AGACCCTGGG AGGGATGAGG AGGACCTGGC GGATGAAATG 
ATATGCATCA CCACCTTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_017007141.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862630.1 Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X6, mRNA.

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CloneID	OHu94543
Clone ID Related Accession (Same CDS sequence)	XM_017007141.1
Accession Version	XM_017007141.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGGG AGCCGCTGCC GCTGTGGGGC TCCCCTCCCC AGCTCTGGCC CGGGCACCAC 
GTGCAGCTTC CACGCCGGCC GGGGCTGGGT CGAGGGAGGA TGGAGCAGCT CCTGGCCAGC 
CCTCCCCTTT GGAGACCGCC AGTGCCCCCC AACCCCTGTC GGCTGGAAGC CTTTCCGGAG 
GCAAACTATG CAAGAGGCAC CAGACTTCCT CTTTCTGGTG AAGGACCAAC TTCTCAGCCG 
AATAGCTCCA AGCAAACTGT CCTGTCTTGG CAAGCTGCAA TCGATGCTGC TAGACAGGCC 
AAGGCTGCCC AAACTATGAG CACCTCTGCA CCCCCACCTG TAGGATCTCT CTCCCAAAGA 
AAACGTCAGC AATACGCCAA GAGCAAAAAA CAGGGTAACT CGTCCAACAG CCGACCTGCC 
CGCGCCCTTT TCTGTTTATC ACTCAATAAC CCCATCCGAA GAGCCTGCAT TAGTATAGTG 
GAATGGAAAC CATTTGACAT ATTTATATTA TTGGCTATTT TTGCCAATTG TGTGGCCTTA 
GCTATTTACA TCCCATTCCC TGAAGATGAT TCTAATTCAA CAAATCATAA CTTGGAAAAA 
GTAGAATATG CCTTCCTGAT TATTTTTACA GTCGAGACAT TTTTGAAGAT TATAGCGTAT 
GGATTATTGC TACATCCTAA TGCTTATGTT AGGAATGGAT GGAATTTACT GGATTTTGTT 
ATAGTAATAG TAGGATTGTT TAGTGTAATT TTGGAACAAT TAACCAAAGA AACAGAAGGC 
GGGAACCACT CAAGCGGCAA ATCTGGAGGC TTTGATGTCA AAGCCCTCCG TGCCTTTCGA 
GTGTTGCGAC CACTTCGACT AGTGTCAGGA GTGCCCAGTT TACAAGTTGT CCTGAACTCC 
ATTATAAAAG CCATGGTTCC CCTCCTTCAC ATAGCCCTTT TGGTATTATT TGTAATCATA 
ATCTATGCTA TTATAGGATT GGAACTTTTT ATTGGAAAAA TGCACAAAAC ATGTTTTTTT 
GCTGACTCAG ATATCGTAGC TGAAGAGGAC CCAGCTCCAT GTGCGTTCTC AGGGAATGGA 
CGCCAGTGTA CTGCCAATGG CACGGAATGT AGGAGTGGCT GGGTTGGCCC GAACGGAGGC 
ATCACCAACT TTGATAACTT TGCCTTTGCC ATGCTTACTG TGTTTCAGTG CATCACCATG 
GAGGGCTGGA CAGATGTGCT CTACTGGATG AATGATGCTA TGGGATTTGA ATTGCCCTGG 
GTGTATTTTG TCAGTCTCGT CATCTTTGGG TCATTTTTCG TACTAAATCT TGTACTTGGT 
GTATTGAGCG GAGAATTCTC AAAGGAAAGA GAGAAGGCAA AAGCACGGGG AGATTTCCAG 
AAGCTCCGGG AGAAGCAGCA GCTGGAGGAG GATCTAAAGG GCTACTTGGA TTGGATCACC 
CAAGCTGAGG ACATCGATCC GGAGAATGAG GAAGAAGGAG GAGAGGAAGG CAAACGAAAT 
ACTAGCATGC CCACCAGCGA GACTGAGTCT GTGAACACAG AGAACGTCAG CGGTGAAGGC 
GAGAACCGAG GCTGCTGTGG AAGTCTCTGT CAAGCCATCT CAAAATCCAA ACTCAGCCGA 
CGCTGGCGTC GCTGGAACCG ATTCAATCGC AGAAGATGTA GGGCCGCCGT GAAGTCTGTC 
ACGTTTTACT GGCTGGTTAT CGTCCTGGTG TTTCTGAACA CCTTAACCAT TTCCTCTGAG 
CACTACAATC AGCCAGATTG GTTGACACAG ATTCAAGATA TTGCCAACAA AGTCCTCTTG 
GCTCTGTTCA CCTGCGAGAT GCTGGTAAAA ATGTACAGCT TGGGCCTCCA AGCATATTTC 
GTCTCTCTTT TCAACCGGTT TGATTGCTTC GTGGTGTGTG GTGGAATCAC TGAGACGATC 
TTGGTGGAAC TGGAAATCAT GTCTCCCCTG GGGATCTCTG TGTTTCGGTG TGTGCGCCTC 
TTAAGAATCT TCAAAGTGAC CAGGCACTGG ACTTCCCTGA GCAACTTAGT GGCATCCTTA 
TTAAACTCCA TGAAGTCCAT CGCTTCGCTG TTGCTTCTGC TTTTTCTCTT CATTATCATC 
TTTTCCTTGC TTGGGATGCA GCTGTTTGGC GGCAAGTTTA ATTTTGATGA AACGCAAACC 
AAGCGGAGCA CCTTTGACAA TTTCCCTCAA GCACTTCTCA CAGTGTTCCA GATCCTGACA 
GGCGAAGACT GGAATGCTGT GATGTACGAT GGCATCATGG CTTACGGGGG CCCATCCTCT 
TCAGGAATGA TCGTCTGCAT CTACTTCATC ATCCTCTTCA TTTGTGGTAA CTATATTCTA 
CTGAATGTCT TCTTGGCCAT CGCTGTAGAC AATTTGGCTG ATGCTGAAAG TCTGAACACT 
GCTCAGAAAG AAGAAGCGGA AGAAAAGGAG AGGAAAAAGA TTGCCAGAAA AGAGAGCCTA 
GAAAATAAAA AGAACAACAA ACCAGAAGTC AACCAGATAG CCAACAGTGA CAACAAGGTT 
ACAATTGATG ACTATAGAGA AGAGGATGAA GACAAGGACC CCTATCCGCC TTGCGATGTG 
CCAGTAGGGG AAGAGGAAGA GGAAGAGGAG GAGGATGAAC CTGAGGTTCC TGCCGGACCC 
CGTCCTCGAA GGATCTCGGA GTTGAACATG AAGGAAAAAA TTGCCCCCAT CCCTGAAGGG 
AGCGCTTTCT TCATTCTTAG CAAGACCAAC CCGATCCGCG TAGGCTGCCA CAAGCTCATC 
AACCACCACA TCTTCACCAA CCTCATCCTT GTCTTCATCA TGCTGAGCAG CGCTGCCCTG 
GCCGCAGAGG ACCCCATCCG CAGCCACTCC TTCCGGAACA CGATACTGGG TTACTTTGAC 
TATGCCTTCA CAGCCATCTT TACTGTTGAG ATCCTGTTGA AGATGACAAC TTTTGGAGCT 
TTCCTCCACA AAGGGGCCTT CTGCAGGAAC TACTTCAATT TGCTGGATAT GCTGGTGGTT 
GGGGTGTCTC TGGTGTCATT TGGGATTCAA TCCAGTGCCA TCTCCGTTGT GAAGATTCTG 
AGGGTCTTAA GGGTCCTGCG TCCCCTCAGG GCCATCAACA GAGCAAAAGG ACTTAAGCAC 
GTGGTCCAGT GCGTCTTCGT GGCCATCCGG ACCATCGGCA ACATCATGAT CGTCACCACC 
CTCCTGCAGT TCATGTTTGC CTGTATCGGG GTCCAGTTGT TCAAGGGGAA GTTCTATCGC 
TGTACGGATG AAGCCAAAAG TAACCCTGAA GAATGCAGGG GACTTTTCAT CCTCTACAAG 
GATGGGGATG TTGACAGTCC TGTGGTCCGT GAACGGATCT GGCAAAACAG TGATTTCAAC 
TTCGACAACG TCCTCTCTGC TATGATGGCG CTCTTCACAG TCTCCACGTT TGAGGGCTGG 
CCTGCGTTGC TGTATAAAGC CATCGACTCG AATGGAGAGA ACATCGGCCC AATCTACAAC 
CACCGCGTGG AGATCTCCAT CTTCTTCATC ATCTACATCA TCATTGTAGC TTTCTTCATG 
ATGAACATCT TTGTGGGCTT TGTCATCGTT ACATTTCAGG AACAAGGAGA AAAAGAGTAT 
AAGAACTGTG AGCTGGACAA AAATCAGCGT CAGTGTGTTG AATACGCCTT GAAAGCACGT 
CCCTTGCGGA GATACATCCC CAAAAACCCC TACCAGTACA AGTTCTGGTA CGTGGTGAAC 
TCTTCGCCTT TCGAATACAT GATGTTTGTC CTCATCATGC TCAACACACT CTGCTTGGCC 
ATGCAGCACT ACGAGCAGTC CAAGATGTTC AATGATGCCA TGGACATTCT GAACATGGTC 
TTCACCGGGG TGTTCACCGT CGAGATGGTT TTGAAAGTCA TCGCATTTAA GCCTAAGGGG 
TATTTTAGTG ACGCCTGGAA CACGTTTGAC TCCCTCATCG TAATCGGCAG CATTATAGAC 
GTGGCCCTCA GCGAAGCAGA CCACTATTTC ACTGATGCAT GGAACACTTT TGATGCCTTA 
ATTGTTGTTG GTAGCGTCGT TGATATTGCT ATAACTGAAG TGAATCCAAC TGAAAGTGAA 
AATGTCCCTG TCCCAACTGC TACACCTGGG AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGA TTGGTGAAGC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACAT TGCTGTGGAC TTTTATTAAG TCCTTTCAGG CGCTCCCGTA TGTGGCCCTC 
CTCATAGCCA TGCTGTTCTT CATCTATGCG GTCATTGGCA TGCAGATGTT TGGGAAAGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAATAACT TCCAGACGTT TCCCCAGGCG 
GTGCTGCTGC TCTTCAGGTG TGCAACAGGT GAGGCCTGGC AGGAGATCAT GCTGGCCTGT 
CTCCCAGGGA AGCTCTGTGA CCCTGAGTCA GATTACAACC CCGGGGAGGA GTATACATGT 
GGGAGCAACT TTGCCATTGT CTATTTCATC AGTTTTTACA TGCTCTGTGC ATTTCTGATC 
ATCAATCTGT TTGTGGCTGT CATCATGGAT AATTTCGACT ATCTGACCCG GGACTGGTCT 
ATTTTGGGGC CTCACCATTT AGATGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAG 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTCTGC TTCGACGCAT CCAGCCTCCC 
CTGGGGTTTG GGAAGTTATG TCCACACAGG GTAGCGTGCA AGAGATTAGT TGCCATGAAC 
ATGCCTCTCA ACAGTGACGG GACAGTCATG TTTAATGCAA CCCTGTTTGC TTTGGTTCGA 
ACGGCTCTTA AGATCAAGAC CGAAGGGAAC CTGGAGCAAG CTAATGAAGA ACTTCGGGCT 
GTGATAAAGA AAATTTGGAA GAAAACCAGC ATGAAATTAC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAGTACCC TGCGAAGAAC 
ACCACAATTG CCCTACAGGC GGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CGTGTGATTT GCAAGATGAC GAGCCTGAGG AAACAAAACG AGAAGAAGAA 
GATGATGTGT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ATTCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAATTC CACCTGCAAG TGATACTGAG AAACCGCTGT TTCCTCCAGC AGGAAATTCG 
GTGTGTCATA ACCATCATAA CCATAATTCC ATAGGAAAGC AAGTTCCCAC CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTCCAAA GCTGCCCATG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC ATGTGTCTGA AAATGGGCAT CATTCTTCCC ACAAGCATGA CCGGGAGCCT 
CAGAGAAGGT CCAGTGTGAA AAGAACCCGC TATTATGAAA CTTACATTAG GTCCGACTCA 
GGAGATGAAC AGCTCCCAAC TATTTGCCGG GAAGACCCAG AGATACATGG CTATTTCAGG 
GACCCCCACT GCTTGGGGGA GCAGGAGTAT TTCAGTAGTG AGGAATGCTA CGAGGATGAC 
AGCTCGCCCA CCTGGAGCAG GCAAAACTAT GGCTACTACA GCAGATACCC AGGCAGAAAC 
ATCGACTCTG AGAGGCCCCG AGGCTACCAT CATCCCCAAG GATTCTTGGA GGACGATGAC 
TCGCCCGTTT GCTATGATTC ACGGAGATCT CCAAGGAGAC GCCTACTACC TCCCACCCCA 
GCATCCCACC GGAGATCCTC CTTCAACTTT GAGTGCCTGC GCCGGCAGAG CAGCCAGGAA 
GAGGTCCCGT CGTCTCCCAT CTTCCCCCAT CGCACGGCCC TGCCTCTGCA TCTAATGCAG 
CAACAGATCA TGGCAGTTGC CGGCCTAGAT TCAAGTAAAG CCCAGAAGTA CTCACCGAGT 
CACTCGACCC GGTCGTGGGC CACCCCTCCA GCAACCCCTC CCTACCGGGA CTGGACACCG 
TGCTACACCC CCCTGATCCA AGTGGAGCAG TCAGAGGCCC TGGACCAGGT GAACGGCAGC 
CTGCCGTCCC TGCACCGCAG CTCCTGGTAC ACAGACGAGC CCGACATCTC CTACCGGACT 
TTCACACCAG CCAGCCTGAC TGTCCCCAGC AGCTTCCGGA ACAAAAACAG CGACAAGCAG 
AGGAGTGCGG ACAGCTTGGT GGAGGCAGTC CTGATATCCG AAGGCTTGGG ACGCTATGCA 
AGGGACCCAA AATTTGTGTC AGCAACAAAA CACGAAATCG CTGATGCCTG TGACCTCACC 
ATCGACGAGA TGGAGAGTGC AGCCAGCACC CTGCTTAATG GGAACGTGCG TCCCCGAGCC 
AACGGGGATG TGGGCCCCCT CTCACACCGG CAGGACTATG AGCTACAGGA CTTTGGTCCT 
GGCTACAGCG ACGAAGAGCC AGACCCTGGG AGGGATGAGG AGGACCTGGC GGATGAAATG 
ATATGCATCA CCACCTTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862630.1
CDS	16..6696
Translation	MPGEPLPLWGSPPQLWPGHHVQLPRRPGLGRGRMEQLLASPPLWRPPVPPNPCRLEAFPEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGENRGCCGSLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENIGPIYNHRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPTESENVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEDDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGNLEHVSENGHHSSHKHDREPQRRSSVKRTRYYETYIRSDSGDEQLPTICREDPEIHGYFRDPHCLGEQEYFSSEECYEDDSSPTWSRQNYGYYSRYPGRNIDSERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPASHRRSSFNFECLRRQSSQEEVPSSPIFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVEQSEALDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGNVRPRANGDVGPLSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017007141.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007141.1

ORF Insert Sequence:

ATGCCCGGGG AGCCGCTGCC GCTGTGGGGC TCCCCTCCCC AGCTCTGGCC CGGGCACCAC 
GTGCAGCTTC CACGCCGGCC GGGGCTGGGT CGAGGGAGGA TGGAGCAGCT CCTGGCCAGC 
CCTCCCCTTT GGAGACCGCC AGTGCCCCCC AACCCCTGTC GGCTGGAAGC CTTTCCGGAG 
GCAAACTATG CAAGAGGCAC CAGACTTCCT CTTTCTGGTG AAGGACCAAC TTCTCAGCCG 
AATAGCTCCA AGCAAACTGT CCTGTCTTGG CAAGCTGCAA TCGATGCTGC TAGACAGGCC 
AAGGCTGCCC AAACTATGAG CACCTCTGCA CCCCCACCTG TAGGATCTCT CTCCCAAAGA 
AAACGTCAGC AATACGCCAA GAGCAAAAAA CAGGGTAACT CGTCCAACAG CCGACCTGCC 
CGCGCCCTTT TCTGTTTATC ACTCAATAAC CCCATCCGAA GAGCCTGCAT TAGTATAGTG 
GAATGGAAAC CATTTGACAT ATTTATATTA TTGGCTATTT TTGCCAATTG TGTGGCCTTA 
GCTATTTACA TCCCATTCCC TGAAGATGAT TCTAATTCAA CAAATCATAA CTTGGAAAAA 
GTAGAATATG CCTTCCTGAT TATTTTTACA GTCGAGACAT TTTTGAAGAT TATAGCGTAT 
GGATTATTGC TACATCCTAA TGCTTATGTT AGGAATGGAT GGAATTTACT GGATTTTGTT 
ATAGTAATAG TAGGATTGTT TAGTGTAATT TTGGAACAAT TAACCAAAGA AACAGAAGGC 
GGGAACCACT CAAGCGGCAA ATCTGGAGGC TTTGATGTCA AAGCCCTCCG TGCCTTTCGA 
GTGTTGCGAC CACTTCGACT AGTGTCAGGA GTGCCCAGTT TACAAGTTGT CCTGAACTCC 
ATTATAAAAG CCATGGTTCC CCTCCTTCAC ATAGCCCTTT TGGTATTATT TGTAATCATA 
ATCTATGCTA TTATAGGATT GGAACTTTTT ATTGGAAAAA TGCACAAAAC ATGTTTTTTT 
GCTGACTCAG ATATCGTAGC TGAAGAGGAC CCAGCTCCAT GTGCGTTCTC AGGGAATGGA 
CGCCAGTGTA CTGCCAATGG CACGGAATGT AGGAGTGGCT GGGTTGGCCC GAACGGAGGC 
ATCACCAACT TTGATAACTT TGCCTTTGCC ATGCTTACTG TGTTTCAGTG CATCACCATG 
GAGGGCTGGA CAGATGTGCT CTACTGGATG AATGATGCTA TGGGATTTGA ATTGCCCTGG 
GTGTATTTTG TCAGTCTCGT CATCTTTGGG TCATTTTTCG TACTAAATCT TGTACTTGGT 
GTATTGAGCG GAGAATTCTC AAAGGAAAGA GAGAAGGCAA AAGCACGGGG AGATTTCCAG 
AAGCTCCGGG AGAAGCAGCA GCTGGAGGAG GATCTAAAGG GCTACTTGGA TTGGATCACC 
CAAGCTGAGG ACATCGATCC GGAGAATGAG GAAGAAGGAG GAGAGGAAGG CAAACGAAAT 
ACTAGCATGC CCACCAGCGA GACTGAGTCT GTGAACACAG AGAACGTCAG CGGTGAAGGC 
GAGAACCGAG GCTGCTGTGG AAGTCTCTGT CAAGCCATCT CAAAATCCAA ACTCAGCCGA 
CGCTGGCGTC GCTGGAACCG ATTCAATCGC AGAAGATGTA GGGCCGCCGT GAAGTCTGTC 
ACGTTTTACT GGCTGGTTAT CGTCCTGGTG TTTCTGAACA CCTTAACCAT TTCCTCTGAG 
CACTACAATC AGCCAGATTG GTTGACACAG ATTCAAGATA TTGCCAACAA AGTCCTCTTG 
GCTCTGTTCA CCTGCGAGAT GCTGGTAAAA ATGTACAGCT TGGGCCTCCA AGCATATTTC 
GTCTCTCTTT TCAACCGGTT TGATTGCTTC GTGGTGTGTG GTGGAATCAC TGAGACGATC 
TTGGTGGAAC TGGAAATCAT GTCTCCCCTG GGGATCTCTG TGTTTCGGTG TGTGCGCCTC 
TTAAGAATCT TCAAAGTGAC CAGGCACTGG ACTTCCCTGA GCAACTTAGT GGCATCCTTA 
TTAAACTCCA TGAAGTCCAT CGCTTCGCTG TTGCTTCTGC TTTTTCTCTT CATTATCATC 
TTTTCCTTGC TTGGGATGCA GCTGTTTGGC GGCAAGTTTA ATTTTGATGA AACGCAAACC 
AAGCGGAGCA CCTTTGACAA TTTCCCTCAA GCACTTCTCA CAGTGTTCCA GATCCTGACA 
GGCGAAGACT GGAATGCTGT GATGTACGAT GGCATCATGG CTTACGGGGG CCCATCCTCT 
TCAGGAATGA TCGTCTGCAT CTACTTCATC ATCCTCTTCA TTTGTGGTAA CTATATTCTA 
CTGAATGTCT TCTTGGCCAT CGCTGTAGAC AATTTGGCTG ATGCTGAAAG TCTGAACACT 
GCTCAGAAAG AAGAAGCGGA AGAAAAGGAG AGGAAAAAGA TTGCCAGAAA AGAGAGCCTA 
GAAAATAAAA AGAACAACAA ACCAGAAGTC AACCAGATAG CCAACAGTGA CAACAAGGTT 
ACAATTGATG ACTATAGAGA AGAGGATGAA GACAAGGACC CCTATCCGCC TTGCGATGTG 
CCAGTAGGGG AAGAGGAAGA GGAAGAGGAG GAGGATGAAC CTGAGGTTCC TGCCGGACCC 
CGTCCTCGAA GGATCTCGGA GTTGAACATG AAGGAAAAAA TTGCCCCCAT CCCTGAAGGG 
AGCGCTTTCT TCATTCTTAG CAAGACCAAC CCGATCCGCG TAGGCTGCCA CAAGCTCATC 
AACCACCACA TCTTCACCAA CCTCATCCTT GTCTTCATCA TGCTGAGCAG CGCTGCCCTG 
GCCGCAGAGG ACCCCATCCG CAGCCACTCC TTCCGGAACA CGATACTGGG TTACTTTGAC 
TATGCCTTCA CAGCCATCTT TACTGTTGAG ATCCTGTTGA AGATGACAAC TTTTGGAGCT 
TTCCTCCACA AAGGGGCCTT CTGCAGGAAC TACTTCAATT TGCTGGATAT GCTGGTGGTT 
GGGGTGTCTC TGGTGTCATT TGGGATTCAA TCCAGTGCCA TCTCCGTTGT GAAGATTCTG 
AGGGTCTTAA GGGTCCTGCG TCCCCTCAGG GCCATCAACA GAGCAAAAGG ACTTAAGCAC 
GTGGTCCAGT GCGTCTTCGT GGCCATCCGG ACCATCGGCA ACATCATGAT CGTCACCACC 
CTCCTGCAGT TCATGTTTGC CTGTATCGGG GTCCAGTTGT TCAAGGGGAA GTTCTATCGC 
TGTACGGATG AAGCCAAAAG TAACCCTGAA GAATGCAGGG GACTTTTCAT CCTCTACAAG 
GATGGGGATG TTGACAGTCC TGTGGTCCGT GAACGGATCT GGCAAAACAG TGATTTCAAC 
TTCGACAACG TCCTCTCTGC TATGATGGCG CTCTTCACAG TCTCCACGTT TGAGGGCTGG 
CCTGCGTTGC TGTATAAAGC CATCGACTCG AATGGAGAGA ACATCGGCCC AATCTACAAC 
CACCGCGTGG AGATCTCCAT CTTCTTCATC ATCTACATCA TCATTGTAGC TTTCTTCATG 
ATGAACATCT TTGTGGGCTT TGTCATCGTT ACATTTCAGG AACAAGGAGA AAAAGAGTAT 
AAGAACTGTG AGCTGGACAA AAATCAGCGT CAGTGTGTTG AATACGCCTT GAAAGCACGT 
CCCTTGCGGA GATACATCCC CAAAAACCCC TACCAGTACA AGTTCTGGTA CGTGGTGAAC 
TCTTCGCCTT TCGAATACAT GATGTTTGTC CTCATCATGC TCAACACACT CTGCTTGGCC 
ATGCAGCACT ACGAGCAGTC CAAGATGTTC AATGATGCCA TGGACATTCT GAACATGGTC 
TTCACCGGGG TGTTCACCGT CGAGATGGTT TTGAAAGTCA TCGCATTTAA GCCTAAGGGG 
TATTTTAGTG ACGCCTGGAA CACGTTTGAC TCCCTCATCG TAATCGGCAG CATTATAGAC 
GTGGCCCTCA GCGAAGCAGA CCACTATTTC ACTGATGCAT GGAACACTTT TGATGCCTTA 
ATTGTTGTTG GTAGCGTCGT TGATATTGCT ATAACTGAAG TGAATCCAAC TGAAAGTGAA 
AATGTCCCTG TCCCAACTGC TACACCTGGG AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGA TTGGTGAAGC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACAT TGCTGTGGAC TTTTATTAAG TCCTTTCAGG CGCTCCCGTA TGTGGCCCTC 
CTCATAGCCA TGCTGTTCTT CATCTATGCG GTCATTGGCA TGCAGATGTT TGGGAAAGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAATAACT TCCAGACGTT TCCCCAGGCG 
GTGCTGCTGC TCTTCAGGTG TGCAACAGGT GAGGCCTGGC AGGAGATCAT GCTGGCCTGT 
CTCCCAGGGA AGCTCTGTGA CCCTGAGTCA GATTACAACC CCGGGGAGGA GTATACATGT 
GGGAGCAACT TTGCCATTGT CTATTTCATC AGTTTTTACA TGCTCTGTGC ATTTCTGATC 
ATCAATCTGT TTGTGGCTGT CATCATGGAT AATTTCGACT ATCTGACCCG GGACTGGTCT 
ATTTTGGGGC CTCACCATTT AGATGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAG 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTCTGC TTCGACGCAT CCAGCCTCCC 
CTGGGGTTTG GGAAGTTATG TCCACACAGG GTAGCGTGCA AGAGATTAGT TGCCATGAAC 
ATGCCTCTCA ACAGTGACGG GACAGTCATG TTTAATGCAA CCCTGTTTGC TTTGGTTCGA 
ACGGCTCTTA AGATCAAGAC CGAAGGGAAC CTGGAGCAAG CTAATGAAGA ACTTCGGGCT 
GTGATAAAGA AAATTTGGAA GAAAACCAGC ATGAAATTAC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAGTACCC TGCGAAGAAC 
ACCACAATTG CCCTACAGGC GGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CGTGTGATTT GCAAGATGAC GAGCCTGAGG AAACAAAACG AGAAGAAGAA 
GATGATGTGT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ATTCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAATTC CACCTGCAAG TGATACTGAG AAACCGCTGT TTCCTCCAGC AGGAAATTCG 
GTGTGTCATA ACCATCATAA CCATAATTCC ATAGGAAAGC AAGTTCCCAC CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTCCAAA GCTGCCCATG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC ATGTGTCTGA AAATGGGCAT CATTCTTCCC ACAAGCATGA CCGGGAGCCT 
CAGAGAAGGT CCAGTGTGAA AAGAACCCGC TATTATGAAA CTTACATTAG GTCCGACTCA 
GGAGATGAAC AGCTCCCAAC TATTTGCCGG GAAGACCCAG AGATACATGG CTATTTCAGG 
GACCCCCACT GCTTGGGGGA GCAGGAGTAT TTCAGTAGTG AGGAATGCTA CGAGGATGAC 
AGCTCGCCCA CCTGGAGCAG GCAAAACTAT GGCTACTACA GCAGATACCC AGGCAGAAAC 
ATCGACTCTG AGAGGCCCCG AGGCTACCAT CATCCCCAAG GATTCTTGGA GGACGATGAC 
TCGCCCGTTT GCTATGATTC ACGGAGATCT CCAAGGAGAC GCCTACTACC TCCCACCCCA 
GCATCCCACC GGAGATCCTC CTTCAACTTT GAGTGCCTGC GCCGGCAGAG CAGCCAGGAA 
GAGGTCCCGT CGTCTCCCAT CTTCCCCCAT CGCACGGCCC TGCCTCTGCA TCTAATGCAG 
CAACAGATCA TGGCAGTTGC CGGCCTAGAT TCAAGTAAAG CCCAGAAGTA CTCACCGAGT 
CACTCGACCC GGTCGTGGGC CACCCCTCCA GCAACCCCTC CCTACCGGGA CTGGACACCG 
TGCTACACCC CCCTGATCCA AGTGGAGCAG TCAGAGGCCC TGGACCAGGT GAACGGCAGC 
CTGCCGTCCC TGCACCGCAG CTCCTGGTAC ACAGACGAGC CCGACATCTC CTACCGGACT 
TTCACACCAG CCAGCCTGAC TGTCCCCAGC AGCTTCCGGA ACAAAAACAG CGACAAGCAG 
AGGAGTGCGG ACAGCTTGGT GGAGGCAGTC CTGATATCCG AAGGCTTGGG ACGCTATGCA 
AGGGACCCAA AATTTGTGTC AGCAACAAAA CACGAAATCG CTGATGCCTG TGACCTCACC 
ATCGACGAGA TGGAGAGTGC AGCCAGCACC CTGCTTAATG GGAACGTGCG TCCCCGAGCC 
AACGGGGATG TGGGCCCCCT CTCACACCGG CAGGACTATG AGCTACAGGA CTTTGGTCCT 
GGCTACAGCG ACGAAGAGCC AGACCCTGGG AGGGATGAGG AGGACCTGGC GGATGAAATG 
ATATGCATCA CCACCTTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_017007142.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862631.1 Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X7, mRNA.

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CloneID	OHu94544
Clone ID Related Accession (Same CDS sequence)	XM_017007142.1
Accession Version	XM_017007142.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGGG AGCCGCTGCC GCTGTGGGGC TCCCCTCCCC AGCTCTGGCC CGGGCACCAC 
GTGCAGCTTC CACGCCGGCC GGGGCTGGGT CGAGGGAGGA TGGAGCAGCT CCTGGCCAGC 
CCTCCCCTTT GGAGACCGCC AGTGCCCCCC AACCCCTGTC GGCTGGAAGC CTTTCCGGAG 
GCAAACTATG CAAGAGGCAC CAGACTTCCT CTTTCTGGTG AAGGACCAAC TTCTCAGCCG 
AATAGCTCCA AGCAAACTGT CCTGTCTTGG CAAGCTGCAA TCGATGCTGC TAGACAGGCC 
AAGGCTGCCC AAACTATGAG CACCTCTGCA CCCCCACCTG TAGGATCTCT CTCCCAAAGA 
AAACGTCAGC AATACGCCAA GAGCAAAAAA CAGGGTAACT CGTCCAACAG CCGACCTGCC 
CGCGCCCTTT TCTGTTTATC ACTCAATAAC CCCATCCGAA GAGCCTGCAT TAGTATAGTG 
GAATGGAAAC CATTTGACAT ATTTATATTA TTGGCTATTT TTGCCAATTG TGTGGCCTTA 
GCTATTTACA TCCCATTCCC TGAAGATGAT TCTAATTCAA CAAATCATAA CTTGGAAAAA 
GTAGAATATG CCTTCCTGAT TATTTTTACA GTCGAGACAT TTTTGAAGAT TATAGCGTAT 
GGATTATTGC TACATCCTAA TGCTTATGTT AGGAATGGAT GGAATTTACT GGATTTTGTT 
ATAGTAATAG TAGGATTGTT TAGTGTAATT TTGGAACAAT TAACCAAAGA AACAGAAGGC 
GGGAACCACT CAAGCGGCAA ATCTGGAGGC TTTGATGTCA AAGCCCTCCG TGCCTTTCGA 
GTGTTGCGAC CACTTCGACT AGTGTCAGGA GTGCCCAGTT TACAAGTTGT CCTGAACTCC 
ATTATAAAAG CCATGGTTCC CCTCCTTCAC ATAGCCCTTT TGGTATTATT TGTAATCATA 
ATCTATGCTA TTATAGGATT GGAACTTTTT ATTGGAAAAA TGCACAAAAC ATGTTTTTTT 
GCTGACTCAG ATATCGTAGC TGAAGAGGAC CCAGCTCCAT GTGCGTTCTC AGGGAATGGA 
CGCCAGTGTA CTGCCAATGG CACGGAATGT AGGAGTGGCT GGGTTGGCCC GAACGGAGGC 
ATCACCAACT TTGATAACTT TGCCTTTGCC ATGCTTACTG TGTTTCAGTG CATCACCATG 
GAGGGCTGGA CAGATGTGCT CTACTGGGTA AATGATGCGA TAGGATGGGA ATGGCCATGG 
GTGTATTTTG TTAGTCTGAT CATCCTTGGC TCATTTTTCG TCCTTAACCT GGTTCTTGGT 
GTCCTTAGTG GAGAATTCTC AAAGGAAAGA GAGAAGGCAA AAGCACGGGG AGATTTCCAG 
AAGCTCCGGG AGAAGCAGCA GCTGGAGGAG GATCTAAAGG GCTACTTGGA TTGGATCACC 
CAAGCTGAGG ACATCGATCC GGAGAATGAG GAAGAAGGAG GAGAGGAAGG CAAACGAAAT 
ACTAGCATGC CCACCAGCGA GACTGAGTCT GTGAACACAG AGAACGTCAG CGGTGAAGGC 
GAGAACCGAG GCTGCTGTGG AAGTCTCTGG TGCTGGTGGA GACGGAGAGG CGCGGCCAAG 
GCGGGGCCCT CTGGGTGTCG GCGGTGGGGT CAAGCCATCT CAAAATCCAA ACTCAGCCGA 
CGCTGGCGTC GCTGGAACCG ATTCAATCGC AGAAGATGTA GGGCCGCCGT GAAGTCTGTC 
ACGTTTTACT GGCTGGTTAT CGTCCTGGTG TTTCTGAACA CCTTAACCAT TTCCTCTGAG 
CACTACAATC AGCCAGATTG GTTGACACAG ATTCAAGATA TTGCCAACAA AGTCCTCTTG 
GCTCTGTTCA CCTGCGAGAT GCTGGTAAAA ATGTACAGCT TGGGCCTCCA AGCATATTTC 
GTCTCTCTTT TCAACCGGTT TGATTGCTTC GTGGTGTGTG GTGGAATCAC TGAGACGATC 
TTGGTGGAAC TGGAAATCAT GTCTCCCCTG GGGATCTCTG TGTTTCGGTG TGTGCGCCTC 
TTAAGAATCT TCAAAGTGAC CAGGCACTGG ACTTCCCTGA GCAACTTAGT GGCATCCTTA 
TTAAACTCCA TGAAGTCCAT CGCTTCGCTG TTGCTTCTGC TTTTTCTCTT CATTATCATC 
TTTTCCTTGC TTGGGATGCA GCTGTTTGGC GGCAAGTTTA ATTTTGATGA AACGCAAACC 
AAGCGGAGCA CCTTTGACAA TTTCCCTCAA GCACTTCTCA CAGTGTTCCA GATCCTGACA 
GGCGAAGACT GGAATGCTGT GATGTACGAT GGCATCATGG CTTACGGGGG CCCATCCTCT 
TCAGGAATGA TCGTCTGCAT CTACTTCATC ATCCTCTTCA TTTGTGGTAA CTATATTCTA 
CTGAATGTCT TCTTGGCCAT CGCTGTAGAC AATTTGGCTG ATGCTGAAAG TCTGAACACT 
GCTCAGAAAG AAGAAGCGGA AGAAAAGGAG AGGAAAAAGA TTGCCAGAAA AGAGAGCCTA 
GAAAATAAAA AGAACAACAA ACCAGAAGTC AACCAGATAG CCAACAGTGA CAACAAGGTT 
ACAATTGATG ACTATAGAGA AGAGGATGAA GACAAGGACC CCTATCCGCC TTGCGATGTG 
CCAGTAGGGG AAGAGGAAGA GGAAGAGGAG GAGGATGAAC CTGAGGTTCC TGCCGGACCC 
CGTCCTCGAA GGATCTCGGA GTTGAACATG AAGGAAAAAA TTGCCCCCAT CCCTGAAGGG 
AGCGCTTTCT TCATTCTTAG CAAGACCAAC CCGATCCGCG TAGGCTGCCA CAAGCTCATC 
AACCACCACA TCTTCACCAA CCTCATCCTT GTCTTCATCA TGCTGAGCAG CGCTGCCCTG 
GCCGCAGAGG ACCCCATCCG CAGCCACTCC TTCCGGAACA CGATACTGGG TTACTTTGAC 
TATGCCTTCA CAGCCATCTT TACTGTTGAG ATCCTGTTGA AGATGACAAC TTTTGGAGCT 
TTCCTCCACA AAGGGGCCTT CTGCAGGAAC TACTTCAATT TGCTGGATAT GCTGGTGGTT 
GGGGTGTCTC TGGTGTCATT TGGGATTCAA TCCAGTGCCA TCTCCGTTGT GAAGATTCTG 
AGGGTCTTAA GGGTCCTGCG TCCCCTCAGG GCCATCAACA GAGCAAAAGG ACTTAAGCAC 
GTGGTCCAGT GCGTCTTCGT GGCCATCCGG ACCATCGGCA ACATCATGAT CGTCACCACC 
CTCCTGCAGT TCATGTTTGC CTGTATCGGG GTCCAGTTGT TCAAGGGGAA GTTCTATCGC 
TGTACGGATG AAGCCAAAAG TAACCCTGAA GAATGCAGGG GACTTTTCAT CCTCTACAAG 
GATGGGGATG TTGACAGTCC TGTGGTCCGT GAACGGATCT GGCAAAACAG TGATTTCAAC 
TTCGACAACG TCCTCTCTGC TATGATGGCG CTCTTCACAG TCTCCACGTT TGAGGGCTGG 
CCTGCGTTGC TGTATAAAGC CATCGACTCG AATGGAGAGA ACATCGGCCC AATCTACAAC 
CACCGCGTGG AGATCTCCAT CTTCTTCATC ATCTACATCA TCATTGTAGC TTTCTTCATG 
ATGAACATCT TTGTGGGCTT TGTCATCGTT ACATTTCAGG AACAAGGAGA AAAAGAGTAT 
AAGAACTGTG AGCTGGACAA AAATCAGCGT CAGTGTGTTG AATACGCCTT GAAAGCACGT 
CCCTTGCGGA GATACATCCC CAAAAACCCC TACCAGTACA AGTTCTGGTA CGTGGTGAAC 
TCTTCGCCTT TCGAATACAT GATGTTTGTC CTCATCATGC TCAACACACT CTGCTTGGCC 
ATGCAGCACT ACGAGCAGTC CAAGATGTTC AATGATGCCA TGGACATTCT GAACATGGTC 
TTCACCGGGG TGTTCACCGT CGAGATGGTT TTGAAAGTCA TCGCATTTAA GCCTAAGGGG 
TATTTTAGTG ACGCCTGGAA CACGTTTGAC TCCCTCATCG TAATCGGCAG CATTATAGAC 
GTGGCCCTCA GCGAAGCAGA CCCAACTGAA AGTGAAAATG TCCCTGTCCC AACTGCTACA 
CCTGGGAACT CTGAAGAGAG CAATAGAATC TCCATCACCT TTTTCCGTCT TTTCCGAGTG 
ATGCGATTGG TGAAGCTTCT CAGCAGGGGG GAAGGCATCC GGACATTGCT GTGGACTTTT 
ATTAAGTCCT TTCAGGCGCT CCCGTATGTG GCCCTCCTCA TAGCCATGCT GTTCTTCATC 
TATGCGGTCA TTGGCATGCA GATGTTTGGG AAAGTTGCCA TGAGAGATAA CAACCAGATC 
AATAGGAACA ATAACTTCCA GACGTTTCCC CAGGCGGTGC TGCTGCTCTT CAGGTGTGCA 
ACAGGTGAGG CCTGGCAGGA GATCATGCTG GCCTGTCTCC CAGGGAAGCT CTGTGACCCT 
GAGTCAGATT ACAACCCCGG GGAGGAGTAT ACATGTGGGA GCAACTTTGC CATTGTCTAT 
TTCATCAGTT TTTACATGCT CTGTGCATTT CTGATCATCA ATCTGTTTGT GGCTGTCATC 
ATGGATAATT TCGACTATCT GACCCGGGAC TGGTCTATTT TGGGGCCTCA CCATTTAGAT 
GAATTCAAAA GAATATGGTC AGAATATGAC CCTGAGGCAA AGGGAAGGAT AAAACACCTT 
GATGTGGTCA CTCTGCTTCG ACGCATCCAG CCTCCCCTGG GGTTTGGGAA GTTATGTCCA 
CACAGGGTAG CGTGCAAGAG ATTAGTTGCC ATGAACATGC CTCTCAACAG TGACGGGACA 
GTCATGTTTA ATGCAACCCT GTTTGCTTTG GTTCGAACGG CTCTTAAGAT CAAGACCGAA 
GGGAACCTGG AGCAAGCTAA TGAAGAACTT CGGGCTGTGA TAAAGAAAAT TTGGAAGAAA 
ACCAGCATGA AATTACTTGA CCAAGTTGTC CCTCCAGCTG GTGATGATGA GGTAACCGTG 
GGGAAGTTCT ATGCCACTTT CCTGATACAG GACTACTTTA GGAAATTCAA GAAACGGAAA 
GAACAAGGAC TGGTGGGAAA GTACCCTGCG AAGAACACCA CAATTGCCCT ACAGGCGGGA 
TTAAGGACAC TGCATGACAT TGGGCCAGAA ATCCGGCGTG CTATATCGTG TGATTTGCAA 
GATGACGAGC CTGAGGAAAC AAAACGAGAA GAAGAAGATG ATGTGTTCAA AAGAAATGGT 
GCCCTGCTTG GAAACCATGT CAATCATGTT AATAGTGATA GGAGAGATTC CCTTCAGCAG 
ACCAATACCA CCCACCGTCC CCTGCATGTC CAAAGGCCTT CAATTCCACC TGCAAGTGAT 
ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA AATTCGGTGT GTCATAACCA TCATAACCAT 
AATTCCATAG GAAAGCAAGT TCCCACCTCA ACAAATGCCA ATCTCAATAA TGCCAATATG 
TCCAAAGCTG CCCATGGAAA GCGGCCCAGC ATTGGGAACC TTGAGCATGT GTCTGAAAAT 
GGGCATCATT CTTCCCACAA GCATGACCGG GAGCCTCAGA GAAGGTCCAG TGTGAAAAGA 
ACCCGCTATT ATGAAACTTA CATTAGGTCC GACTCAGGAG ATGAACAGCT CCCAACTATT 
TGCCGGGAAG ACCCAGAGAT ACATGGCTAT TTCAGGGACC CCCACTGCTT GGGGGAGCAG 
GAGTATTTCA GTAGTGAGGA ATGCTACGAG GATGACAGCT CGCCCACCTG GAGCAGGCAA 
AACTATGGCT ACTACAGCAG ATACCCAGGC AGAAACATCG ACTCTGAGAG GCCCCGAGGC 
TACCATCATC CCCAAGGATT CTTGGAGGAC GATGACTCGC CCGTTTGCTA TGATTCACGG 
AGATCTCCAA GGAGACGCCT ACTACCTCCC ACCCCAGCAT CCCACCGGAG ATCCTCCTTC 
AACTTTGAGT GCCTGCGCCG GCAGAGCAGC CAGGAAGAGG TCCCGTCGTC TCCCATCTTC 
CCCCATCGCA CGGCCCTGCC TCTGCATCTA ATGCAGCAAC AGATCATGGC AGTTGCCGGC 
CTAGATTCAA GTAAAGCCCA GAAGTACTCA CCGAGTCACT CGACCCGGTC GTGGGCCACC 
CCTCCAGCAA CCCCTCCCTA CCGGGACTGG ACACCGTGCT ACACCCCCCT GATCCAAGTG 
GAGCAGTCAG AGGCCCTGGA CCAGGTGAAC GGCAGCCTGC CGTCCCTGCA CCGCAGCTCC 
TGGTACACAG ACGAGCCCGA CATCTCCTAC CGGACTTTCA CACCAGCCAG CCTGACTGTC 
CCCAGCAGCT TCCGGAACAA AAACAGCGAC AAGCAGAGGA GTGCGGACAG CTTGGTGGAG 
GCAGTCCTGA TATCCGAAGG CTTGGGACGC TATGCAAGGG ACCCAAAATT TGTGTCAGCA 
ACAAAACACG AAATCGCTGA TGCCTGTGAC CTCACCATCG ACGAGATGGA GAGTGCAGCC 
AGCACCCTGC TTAATGGGAA CGTGCGTCCC CGAGCCAACG GGGATGTGGG CCCCCTCTCA 
CACCGGCAGG ACTATGAGCT ACAGGACTTT GGTCCTGGCT ACAGCGACGA AGAGCCAGAC 
CCTGGGAGGG ATGAGGAGGA CCTGGCGGAT GAAATGATAT GCATCACCAC CTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862631.1
CDS	16..6672
Translation	MPGEPLPLWGSPPQLWPGHHVQLPRRPGLGRGRMEQLLASPPLWRPPVPPNPCRLEAFPEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGENRGCCGSLWCWWRRRGAAKAGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENIGPIYNHRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADPTESENVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEDDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGNLEHVSENGHHSSHKHDREPQRRSSVKRTRYYETYIRSDSGDEQLPTICREDPEIHGYFRDPHCLGEQEYFSSEECYEDDSSPTWSRQNYGYYSRYPGRNIDSERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPASHRRSSFNFECLRRQSSQEEVPSSPIFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVEQSEALDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGNVRPRANGDVGPLSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017007142.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007142.1

ORF Insert Sequence:

ATGCCCGGGG AGCCGCTGCC GCTGTGGGGC TCCCCTCCCC AGCTCTGGCC CGGGCACCAC 
GTGCAGCTTC CACGCCGGCC GGGGCTGGGT CGAGGGAGGA TGGAGCAGCT CCTGGCCAGC 
CCTCCCCTTT GGAGACCGCC AGTGCCCCCC AACCCCTGTC GGCTGGAAGC CTTTCCGGAG 
GCAAACTATG CAAGAGGCAC CAGACTTCCT CTTTCTGGTG AAGGACCAAC TTCTCAGCCG 
AATAGCTCCA AGCAAACTGT CCTGTCTTGG CAAGCTGCAA TCGATGCTGC TAGACAGGCC 
AAGGCTGCCC AAACTATGAG CACCTCTGCA CCCCCACCTG TAGGATCTCT CTCCCAAAGA 
AAACGTCAGC AATACGCCAA GAGCAAAAAA CAGGGTAACT CGTCCAACAG CCGACCTGCC 
CGCGCCCTTT TCTGTTTATC ACTCAATAAC CCCATCCGAA GAGCCTGCAT TAGTATAGTG 
GAATGGAAAC CATTTGACAT ATTTATATTA TTGGCTATTT TTGCCAATTG TGTGGCCTTA 
GCTATTTACA TCCCATTCCC TGAAGATGAT TCTAATTCAA CAAATCATAA CTTGGAAAAA 
GTAGAATATG CCTTCCTGAT TATTTTTACA GTCGAGACAT TTTTGAAGAT TATAGCGTAT 
GGATTATTGC TACATCCTAA TGCTTATGTT AGGAATGGAT GGAATTTACT GGATTTTGTT 
ATAGTAATAG TAGGATTGTT TAGTGTAATT TTGGAACAAT TAACCAAAGA AACAGAAGGC 
GGGAACCACT CAAGCGGCAA ATCTGGAGGC TTTGATGTCA AAGCCCTCCG TGCCTTTCGA 
GTGTTGCGAC CACTTCGACT AGTGTCAGGA GTGCCCAGTT TACAAGTTGT CCTGAACTCC 
ATTATAAAAG CCATGGTTCC CCTCCTTCAC ATAGCCCTTT TGGTATTATT TGTAATCATA 
ATCTATGCTA TTATAGGATT GGAACTTTTT ATTGGAAAAA TGCACAAAAC ATGTTTTTTT 
GCTGACTCAG ATATCGTAGC TGAAGAGGAC CCAGCTCCAT GTGCGTTCTC AGGGAATGGA 
CGCCAGTGTA CTGCCAATGG CACGGAATGT AGGAGTGGCT GGGTTGGCCC GAACGGAGGC 
ATCACCAACT TTGATAACTT TGCCTTTGCC ATGCTTACTG TGTTTCAGTG CATCACCATG 
GAGGGCTGGA CAGATGTGCT CTACTGGGTA AATGATGCGA TAGGATGGGA ATGGCCATGG 
GTGTATTTTG TTAGTCTGAT CATCCTTGGC TCATTTTTCG TCCTTAACCT GGTTCTTGGT 
GTCCTTAGTG GAGAATTCTC AAAGGAAAGA GAGAAGGCAA AAGCACGGGG AGATTTCCAG 
AAGCTCCGGG AGAAGCAGCA GCTGGAGGAG GATCTAAAGG GCTACTTGGA TTGGATCACC 
CAAGCTGAGG ACATCGATCC GGAGAATGAG GAAGAAGGAG GAGAGGAAGG CAAACGAAAT 
ACTAGCATGC CCACCAGCGA GACTGAGTCT GTGAACACAG AGAACGTCAG CGGTGAAGGC 
GAGAACCGAG GCTGCTGTGG AAGTCTCTGG TGCTGGTGGA GACGGAGAGG CGCGGCCAAG 
GCGGGGCCCT CTGGGTGTCG GCGGTGGGGT CAAGCCATCT CAAAATCCAA ACTCAGCCGA 
CGCTGGCGTC GCTGGAACCG ATTCAATCGC AGAAGATGTA GGGCCGCCGT GAAGTCTGTC 
ACGTTTTACT GGCTGGTTAT CGTCCTGGTG TTTCTGAACA CCTTAACCAT TTCCTCTGAG 
CACTACAATC AGCCAGATTG GTTGACACAG ATTCAAGATA TTGCCAACAA AGTCCTCTTG 
GCTCTGTTCA CCTGCGAGAT GCTGGTAAAA ATGTACAGCT TGGGCCTCCA AGCATATTTC 
GTCTCTCTTT TCAACCGGTT TGATTGCTTC GTGGTGTGTG GTGGAATCAC TGAGACGATC 
TTGGTGGAAC TGGAAATCAT GTCTCCCCTG GGGATCTCTG TGTTTCGGTG TGTGCGCCTC 
TTAAGAATCT TCAAAGTGAC CAGGCACTGG ACTTCCCTGA GCAACTTAGT GGCATCCTTA 
TTAAACTCCA TGAAGTCCAT CGCTTCGCTG TTGCTTCTGC TTTTTCTCTT CATTATCATC 
TTTTCCTTGC TTGGGATGCA GCTGTTTGGC GGCAAGTTTA ATTTTGATGA AACGCAAACC 
AAGCGGAGCA CCTTTGACAA TTTCCCTCAA GCACTTCTCA CAGTGTTCCA GATCCTGACA 
GGCGAAGACT GGAATGCTGT GATGTACGAT GGCATCATGG CTTACGGGGG CCCATCCTCT 
TCAGGAATGA TCGTCTGCAT CTACTTCATC ATCCTCTTCA TTTGTGGTAA CTATATTCTA 
CTGAATGTCT TCTTGGCCAT CGCTGTAGAC AATTTGGCTG ATGCTGAAAG TCTGAACACT 
GCTCAGAAAG AAGAAGCGGA AGAAAAGGAG AGGAAAAAGA TTGCCAGAAA AGAGAGCCTA 
GAAAATAAAA AGAACAACAA ACCAGAAGTC AACCAGATAG CCAACAGTGA CAACAAGGTT 
ACAATTGATG ACTATAGAGA AGAGGATGAA GACAAGGACC CCTATCCGCC TTGCGATGTG 
CCAGTAGGGG AAGAGGAAGA GGAAGAGGAG GAGGATGAAC CTGAGGTTCC TGCCGGACCC 
CGTCCTCGAA GGATCTCGGA GTTGAACATG AAGGAAAAAA TTGCCCCCAT CCCTGAAGGG 
AGCGCTTTCT TCATTCTTAG CAAGACCAAC CCGATCCGCG TAGGCTGCCA CAAGCTCATC 
AACCACCACA TCTTCACCAA CCTCATCCTT GTCTTCATCA TGCTGAGCAG CGCTGCCCTG 
GCCGCAGAGG ACCCCATCCG CAGCCACTCC TTCCGGAACA CGATACTGGG TTACTTTGAC 
TATGCCTTCA CAGCCATCTT TACTGTTGAG ATCCTGTTGA AGATGACAAC TTTTGGAGCT 
TTCCTCCACA AAGGGGCCTT CTGCAGGAAC TACTTCAATT TGCTGGATAT GCTGGTGGTT 
GGGGTGTCTC TGGTGTCATT TGGGATTCAA TCCAGTGCCA TCTCCGTTGT GAAGATTCTG 
AGGGTCTTAA GGGTCCTGCG TCCCCTCAGG GCCATCAACA GAGCAAAAGG ACTTAAGCAC 
GTGGTCCAGT GCGTCTTCGT GGCCATCCGG ACCATCGGCA ACATCATGAT CGTCACCACC 
CTCCTGCAGT TCATGTTTGC CTGTATCGGG GTCCAGTTGT TCAAGGGGAA GTTCTATCGC 
TGTACGGATG AAGCCAAAAG TAACCCTGAA GAATGCAGGG GACTTTTCAT CCTCTACAAG 
GATGGGGATG TTGACAGTCC TGTGGTCCGT GAACGGATCT GGCAAAACAG TGATTTCAAC 
TTCGACAACG TCCTCTCTGC TATGATGGCG CTCTTCACAG TCTCCACGTT TGAGGGCTGG 
CCTGCGTTGC TGTATAAAGC CATCGACTCG AATGGAGAGA ACATCGGCCC AATCTACAAC 
CACCGCGTGG AGATCTCCAT CTTCTTCATC ATCTACATCA TCATTGTAGC TTTCTTCATG 
ATGAACATCT TTGTGGGCTT TGTCATCGTT ACATTTCAGG AACAAGGAGA AAAAGAGTAT 
AAGAACTGTG AGCTGGACAA AAATCAGCGT CAGTGTGTTG AATACGCCTT GAAAGCACGT 
CCCTTGCGGA GATACATCCC CAAAAACCCC TACCAGTACA AGTTCTGGTA CGTGGTGAAC 
TCTTCGCCTT TCGAATACAT GATGTTTGTC CTCATCATGC TCAACACACT CTGCTTGGCC 
ATGCAGCACT ACGAGCAGTC CAAGATGTTC AATGATGCCA TGGACATTCT GAACATGGTC 
TTCACCGGGG TGTTCACCGT CGAGATGGTT TTGAAAGTCA TCGCATTTAA GCCTAAGGGG 
TATTTTAGTG ACGCCTGGAA CACGTTTGAC TCCCTCATCG TAATCGGCAG CATTATAGAC 
GTGGCCCTCA GCGAAGCAGA CCCAACTGAA AGTGAAAATG TCCCTGTCCC AACTGCTACA 
CCTGGGAACT CTGAAGAGAG CAATAGAATC TCCATCACCT TTTTCCGTCT TTTCCGAGTG 
ATGCGATTGG TGAAGCTTCT CAGCAGGGGG GAAGGCATCC GGACATTGCT GTGGACTTTT 
ATTAAGTCCT TTCAGGCGCT CCCGTATGTG GCCCTCCTCA TAGCCATGCT GTTCTTCATC 
TATGCGGTCA TTGGCATGCA GATGTTTGGG AAAGTTGCCA TGAGAGATAA CAACCAGATC 
AATAGGAACA ATAACTTCCA GACGTTTCCC CAGGCGGTGC TGCTGCTCTT CAGGTGTGCA 
ACAGGTGAGG CCTGGCAGGA GATCATGCTG GCCTGTCTCC CAGGGAAGCT CTGTGACCCT 
GAGTCAGATT ACAACCCCGG GGAGGAGTAT ACATGTGGGA GCAACTTTGC CATTGTCTAT 
TTCATCAGTT TTTACATGCT CTGTGCATTT CTGATCATCA ATCTGTTTGT GGCTGTCATC 
ATGGATAATT TCGACTATCT GACCCGGGAC TGGTCTATTT TGGGGCCTCA CCATTTAGAT 
GAATTCAAAA GAATATGGTC AGAATATGAC CCTGAGGCAA AGGGAAGGAT AAAACACCTT 
GATGTGGTCA CTCTGCTTCG ACGCATCCAG CCTCCCCTGG GGTTTGGGAA GTTATGTCCA 
CACAGGGTAG CGTGCAAGAG ATTAGTTGCC ATGAACATGC CTCTCAACAG TGACGGGACA 
GTCATGTTTA ATGCAACCCT GTTTGCTTTG GTTCGAACGG CTCTTAAGAT CAAGACCGAA 
GGGAACCTGG AGCAAGCTAA TGAAGAACTT CGGGCTGTGA TAAAGAAAAT TTGGAAGAAA 
ACCAGCATGA AATTACTTGA CCAAGTTGTC CCTCCAGCTG GTGATGATGA GGTAACCGTG 
GGGAAGTTCT ATGCCACTTT CCTGATACAG GACTACTTTA GGAAATTCAA GAAACGGAAA 
GAACAAGGAC TGGTGGGAAA GTACCCTGCG AAGAACACCA CAATTGCCCT ACAGGCGGGA 
TTAAGGACAC TGCATGACAT TGGGCCAGAA ATCCGGCGTG CTATATCGTG TGATTTGCAA 
GATGACGAGC CTGAGGAAAC AAAACGAGAA GAAGAAGATG ATGTGTTCAA AAGAAATGGT 
GCCCTGCTTG GAAACCATGT CAATCATGTT AATAGTGATA GGAGAGATTC CCTTCAGCAG 
ACCAATACCA CCCACCGTCC CCTGCATGTC CAAAGGCCTT CAATTCCACC TGCAAGTGAT 
ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA AATTCGGTGT GTCATAACCA TCATAACCAT 
AATTCCATAG GAAAGCAAGT TCCCACCTCA ACAAATGCCA ATCTCAATAA TGCCAATATG 
TCCAAAGCTG CCCATGGAAA GCGGCCCAGC ATTGGGAACC TTGAGCATGT GTCTGAAAAT 
GGGCATCATT CTTCCCACAA GCATGACCGG GAGCCTCAGA GAAGGTCCAG TGTGAAAAGA 
ACCCGCTATT ATGAAACTTA CATTAGGTCC GACTCAGGAG ATGAACAGCT CCCAACTATT 
TGCCGGGAAG ACCCAGAGAT ACATGGCTAT TTCAGGGACC CCCACTGCTT GGGGGAGCAG 
GAGTATTTCA GTAGTGAGGA ATGCTACGAG GATGACAGCT CGCCCACCTG GAGCAGGCAA 
AACTATGGCT ACTACAGCAG ATACCCAGGC AGAAACATCG ACTCTGAGAG GCCCCGAGGC 
TACCATCATC CCCAAGGATT CTTGGAGGAC GATGACTCGC CCGTTTGCTA TGATTCACGG 
AGATCTCCAA GGAGACGCCT ACTACCTCCC ACCCCAGCAT CCCACCGGAG ATCCTCCTTC 
AACTTTGAGT GCCTGCGCCG GCAGAGCAGC CAGGAAGAGG TCCCGTCGTC TCCCATCTTC 
CCCCATCGCA CGGCCCTGCC TCTGCATCTA ATGCAGCAAC AGATCATGGC AGTTGCCGGC 
CTAGATTCAA GTAAAGCCCA GAAGTACTCA CCGAGTCACT CGACCCGGTC GTGGGCCACC 
CCTCCAGCAA CCCCTCCCTA CCGGGACTGG ACACCGTGCT ACACCCCCCT GATCCAAGTG 
GAGCAGTCAG AGGCCCTGGA CCAGGTGAAC GGCAGCCTGC CGTCCCTGCA CCGCAGCTCC 
TGGTACACAG ACGAGCCCGA CATCTCCTAC CGGACTTTCA CACCAGCCAG CCTGACTGTC 
CCCAGCAGCT TCCGGAACAA AAACAGCGAC AAGCAGAGGA GTGCGGACAG CTTGGTGGAG 
GCAGTCCTGA TATCCGAAGG CTTGGGACGC TATGCAAGGG ACCCAAAATT TGTGTCAGCA 
ACAAAACACG AAATCGCTGA TGCCTGTGAC CTCACCATCG ACGAGATGGA GAGTGCAGCC 
AGCACCCTGC TTAATGGGAA CGTGCGTCCC CGAGCCAACG GGGATGTGGG CCCCCTCTCA 
CACCGGCAGG ACTATGAGCT ACAGGACTTT GGTCCTGGCT ACAGCGACGA AGAGCCAGAC 
CCTGGGAGGG ATGAGGAGGA CCTGGCGGAT GAAATGATAT GCATCACCAC CTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_017007143.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862632.1 Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X8, mRNA.

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CloneID	OHu94545
Clone ID Related Accession (Same CDS sequence)	XM_017007143.1
Accession Version	XM_017007143.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGGG AGCCGCTGCC GCTGTGGGGC TCCCCTCCCC AGCTCTGGCC CGGGCACCAC 
GTGCAGCTTC CACGCCGGCC GGGGCTGGGT CGAGGGAGGA TGGAGCAGCT CCTGGCCAGC 
CCTCCCCTTT GGAGACCGCC AGTGCCCCCC AACCCCTGTC GGCTGGAAGC CTTTCCGGAG 
GCAAACTATG CAAGAGGCAC CAGACTTCCT CTTTCTGGTG AAGGACCAAC TTCTCAGCCG 
AATAGCTCCA AGCAAACTGT CCTGTCTTGG CAAGCTGCAA TCGATGCTGC TAGACAGGCC 
AAGGCTGCCC AAACTATGAG CACCTCTGCA CCCCCACCTG TAGGATCTCT CTCCCAAAGA 
AAACGTCAGC AATACGCCAA GAGCAAAAAA CAGGGTAACT CGTCCAACAG CCGACCTGCC 
CGCGCCCTTT TCTGTTTATC ACTCAATAAC CCCATCCGAA GAGCCTGCAT TAGTATAGTG 
GAATGGAAAC CATTTGACAT ATTTATATTA TTGGCTATTT TTGCCAATTG TGTGGCCTTA 
GCTATTTACA TCCCATTCCC TGAAGATGAT TCTAATTCAA CAAATCATAA CTTGGAAAAA 
GTAGAATATG CCTTCCTGAT TATTTTTACA GTCGAGACAT TTTTGAAGAT TATAGCGTAT 
GGATTATTGC TACATCCTAA TGCTTATGTT AGGAATGGAT GGAATTTACT GGATTTTGTT 
ATAGTAATAG TAGGATTGTT TAGTGTAATT TTGGAACAAT TAACCAAAGA AACAGAAGGC 
GGGAACCACT CAAGCGGCAA ATCTGGAGGC TTTGATGTCA AAGCCCTCCG TGCCTTTCGA 
GTGTTGCGAC CACTTCGACT AGTGTCAGGA GTGCCCAGTT TACAAGTTGT CCTGAACTCC 
ATTATAAAAG CCATGGTTCC CCTCCTTCAC ATAGCCCTTT TGGTATTATT TGTAATCATA 
ATCTATGCTA TTATAGGATT GGAACTTTTT ATTGGAAAAA TGCACAAAAC ATGTTTTTTT 
GCTGACTCAG ATATCGTAGC TGAAGAGGAC CCAGCTCCAT GTGCGTTCTC AGGGAATGGA 
CGCCAGTGTA CTGCCAATGG CACGGAATGT AGGAGTGGCT GGGTTGGCCC GAACGGAGGC 
ATCACCAACT TTGATAACTT TGCCTTTGCC ATGCTTACTG TGTTTCAGTG CATCACCATG 
GAGGGCTGGA CAGATGTGCT CTACTGGGTA AATGATGCGA TAGGATGGGA ATGGCCATGG 
GTGTATTTTG TTAGTCTGAT CATCCTTGGC TCATTTTTCG TCCTTAACCT GGTTCTTGGT 
GTCCTTAGTG GAGAATTCTC AAAGGAAAGA GAGAAGGCAA AAGCACGGGG AGATTTCCAG 
AAGCTCCGGG AGAAGCAGCA GCTGGAGGAG GATCTAAAGG GCTACTTGGA TTGGATCACC 
CAAGCTGAGG ACATCGATCC GGAGAATGAG GAAGAAGGAG GAGAGGAAGG CAAACGAAAT 
ACTAGCATGC CCACCAGCGA GACTGAGTCT GTGAACACAG AGAACGTCAG CGGTGAAGGC 
GAGAACCGAG GCTGCTGTGG AAGTCTCTGG TGCTGGTGGA GACGGAGAGG CGCGGCCAAG 
GCGGGGCCCT CTGGGTGTCG GCGGTGGGGT CAAGCCATCT CAAAATCCAA ACTCAGCCGA 
CGCTGGCGTC GCTGGAACCG ATTCAATCGC AGAAGATGTA GGGCCGCCGT GAAGTCTGTC 
ACGTTTTACT GGCTGGTTAT CGTCCTGGTG TTTCTGAACA CCTTAACCAT TTCCTCTGAG 
CACTACAATC AGCCAGATTG GTTGACACAG ATTCAAGATA TTGCCAACAA AGTCCTCTTG 
GCTCTGTTCA CCTGCGAGAT GCTGGTAAAA ATGTACAGCT TGGGCCTCCA AGCATATTTC 
GTCTCTCTTT TCAACCGGTT TGATTGCTTC GTGGTGTGTG GTGGAATCAC TGAGACGATC 
TTGGTGGAAC TGGAAATCAT GTCTCCCCTG GGGATCTCTG TGTTTCGGTG TGTGCGCCTC 
TTAAGAATCT TCAAAGTGAC CAGGCACTGG ACTTCCCTGA GCAACTTAGT GGCATCCTTA 
TTAAACTCCA TGAAGTCCAT CGCTTCGCTG TTGCTTCTGC TTTTTCTCTT CATTATCATC 
TTTTCCTTGC TTGGGATGCA GCTGTTTGGC GGCAAGTTTA ATTTTGATGA AACGCAAACC 
AAGCGGAGCA CCTTTGACAA TTTCCCTCAA GCACTTCTCA CAGTGTTCCA GATCCTGACA 
GGCGAAGACT GGAATGCTGT GATGTACGAT GGCATCATGG CTTACGGGGG CCCATCCTCT 
TCAGGAATGA TCGTCTGCAT CTACTTCATC ATCCTCTTCA TTTGTGGTAA CTATATTCTA 
CTGAATGTCT TCTTGGCCAT CGCTGTAGAC AATTTGGCTG ATGCTGAAAG TCTGAACACT 
GCTCAGAAAG AAGAAGCGGA AGAAAAGGAG AGGAAAAAGA TTGCCAGAAA AGAGAGCCTA 
GAAAATAAAA AGAACAACAA ACCAGAAGTC AACCAGATAG CCAACAGTGA CAACAAGGTT 
ACAATTGATG ACTATAGAGA AGAGGATGAA GACAAGGACC CCTATCCGCC TTGCGATGTG 
CCAGTAGGGG AAGAGGAAGA GGAAGAGGAG GAGGATGAAC CTGAGGTTCC TGCCGGACCC 
CGTCCTCGAA GGATCTCGGA GTTGAACATG AAGGAAAAAA TTGCCCCCAT CCCTGAAGGG 
AGCGCTTTCT TCATTCTTAG CAAGACCAAC CCGATCCGCG TAGGCTGCCA CAAGCTCATC 
AACCACCACA TCTTCACCAA CCTCATCCTT GTCTTCATCA TGCTGAGCAG CGCTGCCCTG 
GCCGCAGAGG ACCCCATCCG CAGCCACTCC TTCCGGAACA CGATACTGGG TTACTTTGAC 
TATGCCTTCA CAGCCATCTT TACTGTTGAG ATCCTGTTGA AGATGACAAC TTTTGGAGCT 
TTCCTCCACA AAGGGGCCTT CTGCAGGAAC TACTTCAATT TGCTGGATAT GCTGGTGGTT 
GGGGTGTCTC TGGTGTCATT TGGGATTCAA TCCAGTGCCA TCTCCGTTGT GAAGATTCTG 
AGGGTCTTAA GGGTCCTGCG TCCCCTCAGG GCCATCAACA GAGCAAAAGG ACTTAAGCAC 
GTGGTCCAGT GCGTCTTCGT GGCCATCCGG ACCATCGGCA ACATCATGAT CGTCACCACC 
CTCCTGCAGT TCATGTTTGC CTGTATCGGG GTCCAGTTGT TCAAGGGGAA GTTCTATCGC 
TGTACGGATG AAGCCAAAAG TAACCCTGAA GAATGCAGGG GACTTTTCAT CCTCTACAAG 
GATGGGGATG TTGACAGTCC TGTGGTCCGT GAACGGATCT GGCAAAACAG TGATTTCAAC 
TTCGACAACG TCCTCTCTGC TATGATGGCG CTCTTCACAG TCTCCACGTT TGAGGGCTGG 
CCTGCGTTGC TGTATAAAGC CATCGACTCG AATGGAGAGA ACATCGGCCC AATCTACAAC 
CACCGCGTGG AGATCTCCAT CTTCTTCATC ATCTACATCA TCATTGTAGC TTTCTTCATG 
ATGAACATCT TTGTGGGCTT TGTCATCGTT ACATTTCAGG AACAAGGAGA AAAAGAGTAT 
AAGAACTGTG AGCTGGACAA AAATCAGCGT CAGTGTGTTG AATACGCCTT GAAAGCACGT 
CCCTTGCGGA GATACATCCC CAAAAACCCC TACCAGTACA AGTTCTGGTA CGTGGTGAAC 
TCTTCGCCTT TCGAATACAT GATGTTTGTC CTCATCATGC TCAACACACT CTGCTTGGCC 
ATGCAGCACT ACGAGCAGTC CAAGATGTTC AATGATGCCA TGGACATTCT GAACATGGTC 
TTCACCGGGG TGTTCACCGT CGAGATGGTT TTGAAAGTCA TCGCATTTAA GCCTAAGCAC 
TATTTCACTG ATGCATGGAA CACTTTTGAT GCCTTAATTG TTGTTGGTAG CGTCGTTGAT 
ATTGCTATAA CTGAAGTGAA TCCAACTGAA AGTGAAAATG TCCCTGTCCC AACTGCTACA 
CCTGGGAACT CTGAAGAGAG CAATAGAATC TCCATCACCT TTTTCCGTCT TTTCCGAGTG 
ATGCGATTGG TGAAGCTTCT CAGCAGGGGG GAAGGCATCC GGACATTGCT GTGGACTTTT 
ATTAAGTCCT TTCAGGCGCT CCCGTATGTG GCCCTCCTCA TAGCCATGCT GTTCTTCATC 
TATGCGGTCA TTGGCATGCA GATGTTTGGG AAAGTTGCCA TGAGAGATAA CAACCAGATC 
AATAGGAACA ATAACTTCCA GACGTTTCCC CAGGCGGTGC TGCTGCTCTT CAGGTGTGCA 
ACAGGTGAGG CCTGGCAGGA GATCATGCTG GCCTGTCTCC CAGGGAAGCT CTGTGACCCT 
GAGTCAGATT ACAACCCCGG GGAGGAGTAT ACATGTGGGA GCAACTTTGC CATTGTCTAT 
TTCATCAGTT TTTACATGCT CTGTGCATTT CTGATCATCA ATCTGTTTGT GGCTGTCATC 
ATGGATAATT TCGACTATCT GACCCGGGAC TGGTCTATTT TGGGGCCTCA CCATTTAGAT 
GAATTCAAAA GAATATGGTC AGAATATGAC CCTGAGGCAA AGGGAAGGAT AAAACACCTT 
GATGTGGTCA CTCTGCTTCG ACGCATCCAG CCTCCCCTGG GGTTTGGGAA GTTATGTCCA 
CACAGGGTAG CGTGCAAGAG ATTAGTTGCC ATGAACATGC CTCTCAACAG TGACGGGACA 
GTCATGTTTA ATGCAACCCT GTTTGCTTTG GTTCGAACGG CTCTTAAGAT CAAGACCGAA 
GGGAACCTGG AGCAAGCTAA TGAAGAACTT CGGGCTGTGA TAAAGAAAAT TTGGAAGAAA 
ACCAGCATGA AATTACTTGA CCAAGTTGTC CCTCCAGCTG GTGATGATGA GGTAACCGTG 
GGGAAGTTCT ATGCCACTTT CCTGATACAG GACTACTTTA GGAAATTCAA GAAACGGAAA 
GAACAAGGAC TGGTGGGAAA GTACCCTGCG AAGAACACCA CAATTGCCCT ACAGGCGGGA 
TTAAGGACAC TGCATGACAT TGGGCCAGAA ATCCGGCGTG CTATATCGTG TGATTTGCAA 
GATGACGAGC CTGAGGAAAC AAAACGAGAA GAAGAAGATG ATGTGTTCAA AAGAAATGGT 
GCCCTGCTTG GAAACCATGT CAATCATGTT AATAGTGATA GGAGAGATTC CCTTCAGCAG 
ACCAATACCA CCCACCGTCC CCTGCATGTC CAAAGGCCTT CAATTCCACC TGCAAGTGAT 
ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA AATTCGGTGT GTCATAACCA TCATAACCAT 
AATTCCATAG GAAAGCAAGT TCCCACCTCA ACAAATGCCA ATCTCAATAA TGCCAATATG 
TCCAAAGCTG CCCATGGAAA GCGGCCCAGC ATTGGGAACC TTGAGCATGT GTCTGAAAAT 
GGGCATCATT CTTCCCACAA GCATGACCGG GAGCCTCAGA GAAGGTCCAG TGTGAAAAGA 
ACCCGCTATT ATGAAACTTA CATTAGGTCC GACTCAGGAG ATGAACAGCT CCCAACTATT 
TGCCGGGAAG ACCCAGAGAT ACATGGCTAT TTCAGGGACC CCCACTGCTT GGGGGAGCAG 
GAGTATTTCA GTAGTGAGGA ATGCTACGAG GATGACAGCT CGCCCACCTG GAGCAGGCAA 
AACTATGGCT ACTACAGCAG ATACCCAGGC AGAAACATCG ACTCTGAGAG GCCCCGAGGC 
TACCATCATC CCCAAGGATT CTTGGAGGAC GATGACTCGC CCGTTTGCTA TGATTCACGG 
AGATCTCCAA GGAGACGCCT ACTACCTCCC ACCCCAGCAT CCCACCGGAG ATCCTCCTTC 
AACTTTGAGT GCCTGCGCCG GCAGAGCAGC CAGGAAGAGG TCCCGTCGTC TCCCATCTTC 
CCCCATCGCA CGGCCCTGCC TCTGCATCTA ATGCAGCAAC AGATCATGGC AGTTGCCGGC 
CTAGATTCAA GTAAAGCCCA GAAGTACTCA CCGAGTCACT CGACCCGGTC GTGGGCCACC 
CCTCCAGCAA CCCCTCCCTA CCGGGACTGG ACACCGTGCT ACACCCCCCT GATCCAAGTG 
GAGCAGTCAG AGGCCCTGGA CCAGGTGAAC GGCAGCCTGC CGTCCCTGCA CCGCAGCTCC 
TGGTACACAG ACGAGCCCGA CATCTCCTAC CGGACTTTCA CACCAGCCAG CCTGACTGTC 
CCCAGCAGCT TCCGGAACAA AAACAGCGAC AAGCAGAGGA GTGCGGACAG CTTGGTGGAG 
GCAGTCCTGA TATCCGAAGG CTTGGGACGC TATGCAAGGG ACCCAAAATT TGTGTCAGCA 
ACAAAACACG AAATCGCTGA TGCCTGTGAC CTCACCATCG ACGAGATGGA GAGTGCAGCC 
AGCACCCTGC TTAATGGGAA CGTGCGTCCC CGAGCCAACG GGGATGTGGG CCCCCTCTCA 
CACCGGCAGG ACTATGAGCT ACAGGACTTT GGTCCTGGCT ACAGCGACGA AGAGCCAGAC 
CCTGGGAGGG ATGAGGAGGA CCTGGCGGAT GAAATGATAT GCATCACCAC CTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862632.1
CDS	16..6672
Translation	MPGEPLPLWGSPPQLWPGHHVQLPRRPGLGRGRMEQLLASPPLWRPPVPPNPCRLEAFPEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGENRGCCGSLWCWWRRRGAAKAGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENIGPIYNHRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNPTESENVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEDDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGNLEHVSENGHHSSHKHDREPQRRSSVKRTRYYETYIRSDSGDEQLPTICREDPEIHGYFRDPHCLGEQEYFSSEECYEDDSSPTWSRQNYGYYSRYPGRNIDSERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPASHRRSSFNFECLRRQSSQEEVPSSPIFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVEQSEALDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGNVRPRANGDVGPLSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017007143.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007143.1

ORF Insert Sequence:

ATGCCCGGGG AGCCGCTGCC GCTGTGGGGC TCCCCTCCCC AGCTCTGGCC CGGGCACCAC 
GTGCAGCTTC CACGCCGGCC GGGGCTGGGT CGAGGGAGGA TGGAGCAGCT CCTGGCCAGC 
CCTCCCCTTT GGAGACCGCC AGTGCCCCCC AACCCCTGTC GGCTGGAAGC CTTTCCGGAG 
GCAAACTATG CAAGAGGCAC CAGACTTCCT CTTTCTGGTG AAGGACCAAC TTCTCAGCCG 
AATAGCTCCA AGCAAACTGT CCTGTCTTGG CAAGCTGCAA TCGATGCTGC TAGACAGGCC 
AAGGCTGCCC AAACTATGAG CACCTCTGCA CCCCCACCTG TAGGATCTCT CTCCCAAAGA 
AAACGTCAGC AATACGCCAA GAGCAAAAAA CAGGGTAACT CGTCCAACAG CCGACCTGCC 
CGCGCCCTTT TCTGTTTATC ACTCAATAAC CCCATCCGAA GAGCCTGCAT TAGTATAGTG 
GAATGGAAAC CATTTGACAT ATTTATATTA TTGGCTATTT TTGCCAATTG TGTGGCCTTA 
GCTATTTACA TCCCATTCCC TGAAGATGAT TCTAATTCAA CAAATCATAA CTTGGAAAAA 
GTAGAATATG CCTTCCTGAT TATTTTTACA GTCGAGACAT TTTTGAAGAT TATAGCGTAT 
GGATTATTGC TACATCCTAA TGCTTATGTT AGGAATGGAT GGAATTTACT GGATTTTGTT 
ATAGTAATAG TAGGATTGTT TAGTGTAATT TTGGAACAAT TAACCAAAGA AACAGAAGGC 
GGGAACCACT CAAGCGGCAA ATCTGGAGGC TTTGATGTCA AAGCCCTCCG TGCCTTTCGA 
GTGTTGCGAC CACTTCGACT AGTGTCAGGA GTGCCCAGTT TACAAGTTGT CCTGAACTCC 
ATTATAAAAG CCATGGTTCC CCTCCTTCAC ATAGCCCTTT TGGTATTATT TGTAATCATA 
ATCTATGCTA TTATAGGATT GGAACTTTTT ATTGGAAAAA TGCACAAAAC ATGTTTTTTT 
GCTGACTCAG ATATCGTAGC TGAAGAGGAC CCAGCTCCAT GTGCGTTCTC AGGGAATGGA 
CGCCAGTGTA CTGCCAATGG CACGGAATGT AGGAGTGGCT GGGTTGGCCC GAACGGAGGC 
ATCACCAACT TTGATAACTT TGCCTTTGCC ATGCTTACTG TGTTTCAGTG CATCACCATG 
GAGGGCTGGA CAGATGTGCT CTACTGGGTA AATGATGCGA TAGGATGGGA ATGGCCATGG 
GTGTATTTTG TTAGTCTGAT CATCCTTGGC TCATTTTTCG TCCTTAACCT GGTTCTTGGT 
GTCCTTAGTG GAGAATTCTC AAAGGAAAGA GAGAAGGCAA AAGCACGGGG AGATTTCCAG 
AAGCTCCGGG AGAAGCAGCA GCTGGAGGAG GATCTAAAGG GCTACTTGGA TTGGATCACC 
CAAGCTGAGG ACATCGATCC GGAGAATGAG GAAGAAGGAG GAGAGGAAGG CAAACGAAAT 
ACTAGCATGC CCACCAGCGA GACTGAGTCT GTGAACACAG AGAACGTCAG CGGTGAAGGC 
GAGAACCGAG GCTGCTGTGG AAGTCTCTGG TGCTGGTGGA GACGGAGAGG CGCGGCCAAG 
GCGGGGCCCT CTGGGTGTCG GCGGTGGGGT CAAGCCATCT CAAAATCCAA ACTCAGCCGA 
CGCTGGCGTC GCTGGAACCG ATTCAATCGC AGAAGATGTA GGGCCGCCGT GAAGTCTGTC 
ACGTTTTACT GGCTGGTTAT CGTCCTGGTG TTTCTGAACA CCTTAACCAT TTCCTCTGAG 
CACTACAATC AGCCAGATTG GTTGACACAG ATTCAAGATA TTGCCAACAA AGTCCTCTTG 
GCTCTGTTCA CCTGCGAGAT GCTGGTAAAA ATGTACAGCT TGGGCCTCCA AGCATATTTC 
GTCTCTCTTT TCAACCGGTT TGATTGCTTC GTGGTGTGTG GTGGAATCAC TGAGACGATC 
TTGGTGGAAC TGGAAATCAT GTCTCCCCTG GGGATCTCTG TGTTTCGGTG TGTGCGCCTC 
TTAAGAATCT TCAAAGTGAC CAGGCACTGG ACTTCCCTGA GCAACTTAGT GGCATCCTTA 
TTAAACTCCA TGAAGTCCAT CGCTTCGCTG TTGCTTCTGC TTTTTCTCTT CATTATCATC 
TTTTCCTTGC TTGGGATGCA GCTGTTTGGC GGCAAGTTTA ATTTTGATGA AACGCAAACC 
AAGCGGAGCA CCTTTGACAA TTTCCCTCAA GCACTTCTCA CAGTGTTCCA GATCCTGACA 
GGCGAAGACT GGAATGCTGT GATGTACGAT GGCATCATGG CTTACGGGGG CCCATCCTCT 
TCAGGAATGA TCGTCTGCAT CTACTTCATC ATCCTCTTCA TTTGTGGTAA CTATATTCTA 
CTGAATGTCT TCTTGGCCAT CGCTGTAGAC AATTTGGCTG ATGCTGAAAG TCTGAACACT 
GCTCAGAAAG AAGAAGCGGA AGAAAAGGAG AGGAAAAAGA TTGCCAGAAA AGAGAGCCTA 
GAAAATAAAA AGAACAACAA ACCAGAAGTC AACCAGATAG CCAACAGTGA CAACAAGGTT 
ACAATTGATG ACTATAGAGA AGAGGATGAA GACAAGGACC CCTATCCGCC TTGCGATGTG 
CCAGTAGGGG AAGAGGAAGA GGAAGAGGAG GAGGATGAAC CTGAGGTTCC TGCCGGACCC 
CGTCCTCGAA GGATCTCGGA GTTGAACATG AAGGAAAAAA TTGCCCCCAT CCCTGAAGGG 
AGCGCTTTCT TCATTCTTAG CAAGACCAAC CCGATCCGCG TAGGCTGCCA CAAGCTCATC 
AACCACCACA TCTTCACCAA CCTCATCCTT GTCTTCATCA TGCTGAGCAG CGCTGCCCTG 
GCCGCAGAGG ACCCCATCCG CAGCCACTCC TTCCGGAACA CGATACTGGG TTACTTTGAC 
TATGCCTTCA CAGCCATCTT TACTGTTGAG ATCCTGTTGA AGATGACAAC TTTTGGAGCT 
TTCCTCCACA AAGGGGCCTT CTGCAGGAAC TACTTCAATT TGCTGGATAT GCTGGTGGTT 
GGGGTGTCTC TGGTGTCATT TGGGATTCAA TCCAGTGCCA TCTCCGTTGT GAAGATTCTG 
AGGGTCTTAA GGGTCCTGCG TCCCCTCAGG GCCATCAACA GAGCAAAAGG ACTTAAGCAC 
GTGGTCCAGT GCGTCTTCGT GGCCATCCGG ACCATCGGCA ACATCATGAT CGTCACCACC 
CTCCTGCAGT TCATGTTTGC CTGTATCGGG GTCCAGTTGT TCAAGGGGAA GTTCTATCGC 
TGTACGGATG AAGCCAAAAG TAACCCTGAA GAATGCAGGG GACTTTTCAT CCTCTACAAG 
GATGGGGATG TTGACAGTCC TGTGGTCCGT GAACGGATCT GGCAAAACAG TGATTTCAAC 
TTCGACAACG TCCTCTCTGC TATGATGGCG CTCTTCACAG TCTCCACGTT TGAGGGCTGG 
CCTGCGTTGC TGTATAAAGC CATCGACTCG AATGGAGAGA ACATCGGCCC AATCTACAAC 
CACCGCGTGG AGATCTCCAT CTTCTTCATC ATCTACATCA TCATTGTAGC TTTCTTCATG 
ATGAACATCT TTGTGGGCTT TGTCATCGTT ACATTTCAGG AACAAGGAGA AAAAGAGTAT 
AAGAACTGTG AGCTGGACAA AAATCAGCGT CAGTGTGTTG AATACGCCTT GAAAGCACGT 
CCCTTGCGGA GATACATCCC CAAAAACCCC TACCAGTACA AGTTCTGGTA CGTGGTGAAC 
TCTTCGCCTT TCGAATACAT GATGTTTGTC CTCATCATGC TCAACACACT CTGCTTGGCC 
ATGCAGCACT ACGAGCAGTC CAAGATGTTC AATGATGCCA TGGACATTCT GAACATGGTC 
TTCACCGGGG TGTTCACCGT CGAGATGGTT TTGAAAGTCA TCGCATTTAA GCCTAAGCAC 
TATTTCACTG ATGCATGGAA CACTTTTGAT GCCTTAATTG TTGTTGGTAG CGTCGTTGAT 
ATTGCTATAA CTGAAGTGAA TCCAACTGAA AGTGAAAATG TCCCTGTCCC AACTGCTACA 
CCTGGGAACT CTGAAGAGAG CAATAGAATC TCCATCACCT TTTTCCGTCT TTTCCGAGTG 
ATGCGATTGG TGAAGCTTCT CAGCAGGGGG GAAGGCATCC GGACATTGCT GTGGACTTTT 
ATTAAGTCCT TTCAGGCGCT CCCGTATGTG GCCCTCCTCA TAGCCATGCT GTTCTTCATC 
TATGCGGTCA TTGGCATGCA GATGTTTGGG AAAGTTGCCA TGAGAGATAA CAACCAGATC 
AATAGGAACA ATAACTTCCA GACGTTTCCC CAGGCGGTGC TGCTGCTCTT CAGGTGTGCA 
ACAGGTGAGG CCTGGCAGGA GATCATGCTG GCCTGTCTCC CAGGGAAGCT CTGTGACCCT 
GAGTCAGATT ACAACCCCGG GGAGGAGTAT ACATGTGGGA GCAACTTTGC CATTGTCTAT 
TTCATCAGTT TTTACATGCT CTGTGCATTT CTGATCATCA ATCTGTTTGT GGCTGTCATC 
ATGGATAATT TCGACTATCT GACCCGGGAC TGGTCTATTT TGGGGCCTCA CCATTTAGAT 
GAATTCAAAA GAATATGGTC AGAATATGAC CCTGAGGCAA AGGGAAGGAT AAAACACCTT 
GATGTGGTCA CTCTGCTTCG ACGCATCCAG CCTCCCCTGG GGTTTGGGAA GTTATGTCCA 
CACAGGGTAG CGTGCAAGAG ATTAGTTGCC ATGAACATGC CTCTCAACAG TGACGGGACA 
GTCATGTTTA ATGCAACCCT GTTTGCTTTG GTTCGAACGG CTCTTAAGAT CAAGACCGAA 
GGGAACCTGG AGCAAGCTAA TGAAGAACTT CGGGCTGTGA TAAAGAAAAT TTGGAAGAAA 
ACCAGCATGA AATTACTTGA CCAAGTTGTC CCTCCAGCTG GTGATGATGA GGTAACCGTG 
GGGAAGTTCT ATGCCACTTT CCTGATACAG GACTACTTTA GGAAATTCAA GAAACGGAAA 
GAACAAGGAC TGGTGGGAAA GTACCCTGCG AAGAACACCA CAATTGCCCT ACAGGCGGGA 
TTAAGGACAC TGCATGACAT TGGGCCAGAA ATCCGGCGTG CTATATCGTG TGATTTGCAA 
GATGACGAGC CTGAGGAAAC AAAACGAGAA GAAGAAGATG ATGTGTTCAA AAGAAATGGT 
GCCCTGCTTG GAAACCATGT CAATCATGTT AATAGTGATA GGAGAGATTC CCTTCAGCAG 
ACCAATACCA CCCACCGTCC CCTGCATGTC CAAAGGCCTT CAATTCCACC TGCAAGTGAT 
ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA AATTCGGTGT GTCATAACCA TCATAACCAT 
AATTCCATAG GAAAGCAAGT TCCCACCTCA ACAAATGCCA ATCTCAATAA TGCCAATATG 
TCCAAAGCTG CCCATGGAAA GCGGCCCAGC ATTGGGAACC TTGAGCATGT GTCTGAAAAT 
GGGCATCATT CTTCCCACAA GCATGACCGG GAGCCTCAGA GAAGGTCCAG TGTGAAAAGA 
ACCCGCTATT ATGAAACTTA CATTAGGTCC GACTCAGGAG ATGAACAGCT CCCAACTATT 
TGCCGGGAAG ACCCAGAGAT ACATGGCTAT TTCAGGGACC CCCACTGCTT GGGGGAGCAG 
GAGTATTTCA GTAGTGAGGA ATGCTACGAG GATGACAGCT CGCCCACCTG GAGCAGGCAA 
AACTATGGCT ACTACAGCAG ATACCCAGGC AGAAACATCG ACTCTGAGAG GCCCCGAGGC 
TACCATCATC CCCAAGGATT CTTGGAGGAC GATGACTCGC CCGTTTGCTA TGATTCACGG 
AGATCTCCAA GGAGACGCCT ACTACCTCCC ACCCCAGCAT CCCACCGGAG ATCCTCCTTC 
AACTTTGAGT GCCTGCGCCG GCAGAGCAGC CAGGAAGAGG TCCCGTCGTC TCCCATCTTC 
CCCCATCGCA CGGCCCTGCC TCTGCATCTA ATGCAGCAAC AGATCATGGC AGTTGCCGGC 
CTAGATTCAA GTAAAGCCCA GAAGTACTCA CCGAGTCACT CGACCCGGTC GTGGGCCACC 
CCTCCAGCAA CCCCTCCCTA CCGGGACTGG ACACCGTGCT ACACCCCCCT GATCCAAGTG 
GAGCAGTCAG AGGCCCTGGA CCAGGTGAAC GGCAGCCTGC CGTCCCTGCA CCGCAGCTCC 
TGGTACACAG ACGAGCCCGA CATCTCCTAC CGGACTTTCA CACCAGCCAG CCTGACTGTC 
CCCAGCAGCT TCCGGAACAA AAACAGCGAC AAGCAGAGGA GTGCGGACAG CTTGGTGGAG 
GCAGTCCTGA TATCCGAAGG CTTGGGACGC TATGCAAGGG ACCCAAAATT TGTGTCAGCA 
ACAAAACACG AAATCGCTGA TGCCTGTGAC CTCACCATCG ACGAGATGGA GAGTGCAGCC 
AGCACCCTGC TTAATGGGAA CGTGCGTCCC CGAGCCAACG GGGATGTGGG CCCCCTCTCA 
CACCGGCAGG ACTATGAGCT ACAGGACTTT GGTCCTGGCT ACAGCGACGA AGAGCCAGAC 
CCTGGGAGGG ATGAGGAGGA CCTGGCGGAT GAAATGATAT GCATCACCAC CTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_017007144.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862633.1 Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X9, mRNA.

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CloneID	OHu94546
Clone ID Related Accession (Same CDS sequence)	XM_017007144.1
Accession Version	XM_017007144.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGGG AGCCGCTGCC GCTGTGGGGC TCCCCTCCCC AGCTCTGGCC CGGGCACCAC 
GTGCAGCTTC CACGCCGGCC GGGGCTGGGT CGAGGGAGGA TGGAGCAGCT CCTGGCCAGC 
CCTCCCCTTT GGAGACCGCC AGTGCCCCCC AACCCCTGTC GGCTGGAAGC CTTTCCGGAG 
GCAAACTATG CAAGAGGCAC CAGACTTCCT CTTTCTGGTG AAGGACCAAC TTCTCAGCCG 
AATAGCTCCA AGCAAACTGT CCTGTCTTGG CAAGCTGCAA TCGATGCTGC TAGACAGGCC 
AAGGCTGCCC AAACTATGAG CACCTCTGCA CCCCCACCTG TAGGATCTCT CTCCCAAAGA 
AAACGTCAGC AATACGCCAA GAGCAAAAAA CAGGGTAACT CGTCCAACAG CCGACCTGCC 
CGCGCCCTTT TCTGTTTATC ACTCAATAAC CCCATCCGAA GAGCCTGCAT TAGTATAGTG 
GAATGGAAAC CATTTGACAT ATTTATATTA TTGGCTATTT TTGCCAATTG TGTGGCCTTA 
GCTATTTACA TCCCATTCCC TGAAGATGAT TCTAATTCAA CAAATCATAA CTTGGAAAAA 
GTAGAATATG CCTTCCTGAT TATTTTTACA GTCGAGACAT TTTTGAAGAT TATAGCGTAT 
GGATTATTGC TACATCCTAA TGCTTATGTT AGGAATGGAT GGAATTTACT GGATTTTGTT 
ATAGTAATAG TAGGATTGTT TAGTGTAATT TTGGAACAAT TAACCAAAGA AACAGAAGGC 
GGGAACCACT CAAGCGGCAA ATCTGGAGGC TTTGATGTCA AAGCCCTCCG TGCCTTTCGA 
GTGTTGCGAC CACTTCGACT AGTGTCAGGA GTGCCCAGTT TACAAGTTGT CCTGAACTCC 
ATTATAAAAG CCATGGTTCC CCTCCTTCAC ATAGCCCTTT TGGTATTATT TGTAATCATA 
ATCTATGCTA TTATAGGATT GGAACTTTTT ATTGGAAAAA TGCACAAAAC ATGTTTTTTT 
GCTGACTCAG ATATCGTAGC TGAAGAGGAC CCAGCTCCAT GTGCGTTCTC AGGGAATGGA 
CGCCAGTGTA CTGCCAATGG CACGGAATGT AGGAGTGGCT GGGTTGGCCC GAACGGAGGC 
ATCACCAACT TTGATAACTT TGCCTTTGCC ATGCTTACTG TGTTTCAGTG CATCACCATG 
GAGGGCTGGA CAGATGTGCT CTACTGGATG AATGATGCTA TGGGATTTGA ATTGCCCTGG 
GTGTATTTTG TCAGTCTCGT CATCTTTGGG TCATTTTTCG TACTAAATCT TGTACTTGGT 
GTATTGAGCG GAGAATTCTC AAAGGAAAGA GAGAAGGCAA AAGCACGGGG AGATTTCCAG 
AAGCTCCGGG AGAAGCAGCA GCTGGAGGAG GATCTAAAGG GCTACTTGGA TTGGATCACC 
CAAGCTGAGG ACATCGATCC GGAGAATGAG GAAGAAGGAG GAGAGGAAGG CAAACGAAAT 
ACTAGCATGC CCACCAGCGA GACTGAGTCT GTGAACACAG AGAACGTCAG CGGTGAAGGC 
GAGAACCGAG GCTGCTGTGG AAGTCTCTGG TGCTGGTGGA GACGGAGAGG CGCGGCCAAG 
GCGGGGCCCT CTGGGTGTCG GCGGTGGGGT CAAGCCATCT CAAAATCCAA ACTCAGCCGA 
CGCTGGCGTC GCTGGAACCG ATTCAATCGC AGAAGATGTA GGGCCGCCGT GAAGTCTGTC 
ACGTTTTACT GGCTGGTTAT CGTCCTGGTG TTTCTGAACA CCTTAACCAT TTCCTCTGAG 
CACTACAATC AGCCAGATTG GTTGACACAG ATTCAAGATA TTGCCAACAA AGTCCTCTTG 
GCTCTGTTCA CCTGCGAGAT GCTGGTAAAA ATGTACAGCT TGGGCCTCCA AGCATATTTC 
GTCTCTCTTT TCAACCGGTT TGATTGCTTC GTGGTGTGTG GTGGAATCAC TGAGACGATC 
TTGGTGGAAC TGGAAATCAT GTCTCCCCTG GGGATCTCTG TGTTTCGGTG TGTGCGCCTC 
TTAAGAATCT TCAAAGTGAC CAGGCACTGG ACTTCCCTGA GCAACTTAGT GGCATCCTTA 
TTAAACTCCA TGAAGTCCAT CGCTTCGCTG TTGCTTCTGC TTTTTCTCTT CATTATCATC 
TTTTCCTTGC TTGGGATGCA GCTGTTTGGC GGCAAGTTTA ATTTTGATGA AACGCAAACC 
AAGCGGAGCA CCTTTGACAA TTTCCCTCAA GCACTTCTCA CAGTGTTCCA GATCCTGACA 
GGCGAAGACT GGAATGCTGT GATGTACGAT GGCATCATGG CTTACGGGGG CCCATCCTCT 
TCAGGAATGA TCGTCTGCAT CTACTTCATC ATCCTCTTCA TTTGTGGTAA CTATATTCTA 
CTGAATGTCT TCTTGGCCAT CGCTGTAGAC AATTTGGCTG ATGCTGAAAG TCTGAACACT 
GCTCAGAAAG AAGAAGCGGA AGAAAAGGAG AGGAAAAAGA TTGCCAGAAA AGAGAGCCTA 
GAAAATAAAA AGAACAACAA ACCAGAAGTC AACCAGATAG CCAACAGTGA CAACAAGGTT 
ACAATTGATG ACTATAGAGA AGAGGATGAA GACAAGGACC CCTATCCGCC TTGCGATGTG 
CCAGTAGGGG AAGAGGAAGA GGAAGAGGAG GAGGATGAAC CTGAGGTTCC TGCCGGACCC 
CGTCCTCGAA GGATCTCGGA GTTGAACATG AAGGAAAAAA TTGCCCCCAT CCCTGAAGGG 
AGCGCTTTCT TCATTCTTAG CAAGACCAAC CCGATCCGCG TAGGCTGCCA CAAGCTCATC 
AACCACCACA TCTTCACCAA CCTCATCCTT GTCTTCATCA TGCTGAGCAG CGCTGCCCTG 
GCCGCAGAGG ACCCCATCCG CAGCCACTCC TTCCGGAACA CGATACTGGG TTACTTTGAC 
TATGCCTTCA CAGCCATCTT TACTGTTGAG ATCCTGTTGA AGATGACAAC TTTTGGAGCT 
TTCCTCCACA AAGGGGCCTT CTGCAGGAAC TACTTCAATT TGCTGGATAT GCTGGTGGTT 
GGGGTGTCTC TGGTGTCATT TGGGATTCAA TCCAGTGCCA TCTCCGTTGT GAAGATTCTG 
AGGGTCTTAA GGGTCCTGCG TCCCCTCAGG GCCATCAACA GAGCAAAAGG ACTTAAGCAC 
GTGGTCCAGT GCGTCTTCGT GGCCATCCGG ACCATCGGCA ACATCATGAT CGTCACCACC 
CTCCTGCAGT TCATGTTTGC CTGTATCGGG GTCCAGTTGT TCAAGGGGAA GTTCTATCGC 
TGTACGGATG AAGCCAAAAG TAACCCTGAA GAATGCAGGG GACTTTTCAT CCTCTACAAG 
GATGGGGATG TTGACAGTCC TGTGGTCCGT GAACGGATCT GGCAAAACAG TGATTTCAAC 
TTCGACAACG TCCTCTCTGC TATGATGGCG CTCTTCACAG TCTCCACGTT TGAGGGCTGG 
CCTGCGTTGC TGTATAAAGC CATCGACTCG AATGGAGAGA ACATCGGCCC AATCTACAAC 
CACCGCGTGG AGATCTCCAT CTTCTTCATC ATCTACATCA TCATTGTAGC TTTCTTCATG 
ATGAACATCT TTGTGGGCTT TGTCATCGTT ACATTTCAGG AACAAGGAGA AAAAGAGTAT 
AAGAACTGTG AGCTGGACAA AAATCAGCGT CAGTGTGTTG AATACGCCTT GAAAGCACGT 
CCCTTGCGGA GATACATCCC CAAAAACCCC TACCAGTACA AGTTCTGGTA CGTGGTGAAC 
TCTTCGCCTT TCGAATACAT GATGTTTGTC CTCATCATGC TCAACACACT CTGCTTGGCC 
ATGCAGCACT ACGAGCAGTC CAAGATGTTC AATGATGCCA TGGACATTCT GAACATGGTC 
TTCACCGGGG TGTTCACCGT CGAGATGGTT TTGAAAGTCA TCGCATTTAA GCCTAAGCAC 
TATTTCACTG ATGCATGGAA CACTTTTGAT GCCTTAATTG TTGTTGGTAG CGTCGTTGAT 
ATTGCTATAA CTGAAGTGAA TCCAACTGAA AGTGAAAATG TCCCTGTCCC AACTGCTACA 
CCTGGGAACT CTGAAGAGAG CAATAGAATC TCCATCACCT TTTTCCGTCT TTTCCGAGTG 
ATGCGATTGG TGAAGCTTCT CAGCAGGGGG GAAGGCATCC GGACATTGCT GTGGACTTTT 
ATTAAGTCCT TTCAGGCGCT CCCGTATGTG GCCCTCCTCA TAGCCATGCT GTTCTTCATC 
TATGCGGTCA TTGGCATGCA GATGTTTGGG AAAGTTGCCA TGAGAGATAA CAACCAGATC 
AATAGGAACA ATAACTTCCA GACGTTTCCC CAGGCGGTGC TGCTGCTCTT CAGGTGTGCA 
ACAGGTGAGG CCTGGCAGGA GATCATGCTG GCCTGTCTCC CAGGGAAGCT CTGTGACCCT 
GAGTCAGATT ACAACCCCGG GGAGGAGTAT ACATGTGGGA GCAACTTTGC CATTGTCTAT 
TTCATCAGTT TTTACATGCT CTGTGCATTT CTGATCATCA ATCTGTTTGT GGCTGTCATC 
ATGGATAATT TCGACTATCT GACCCGGGAC TGGTCTATTT TGGGGCCTCA CCATTTAGAT 
GAATTCAAAA GAATATGGTC AGAATATGAC CCTGAGGCAA AGGGAAGGAT AAAACACCTT 
GATGTGGTCA CTCTGCTTCG ACGCATCCAG CCTCCCCTGG GGTTTGGGAA GTTATGTCCA 
CACAGGGTAG CGTGCAAGAG ATTAGTTGCC ATGAACATGC CTCTCAACAG TGACGGGACA 
GTCATGTTTA ATGCAACCCT GTTTGCTTTG GTTCGAACGG CTCTTAAGAT CAAGACCGAA 
GGGAACCTGG AGCAAGCTAA TGAAGAACTT CGGGCTGTGA TAAAGAAAAT TTGGAAGAAA 
ACCAGCATGA AATTACTTGA CCAAGTTGTC CCTCCAGCTG GTGATGATGA GGTAACCGTG 
GGGAAGTTCT ATGCCACTTT CCTGATACAG GACTACTTTA GGAAATTCAA GAAACGGAAA 
GAACAAGGAC TGGTGGGAAA GTACCCTGCG AAGAACACCA CAATTGCCCT ACAGGCGGGA 
TTAAGGACAC TGCATGACAT TGGGCCAGAA ATCCGGCGTG CTATATCGTG TGATTTGCAA 
GATGACGAGC CTGAGGAAAC AAAACGAGAA GAAGAAGATG ATGTGTTCAA AAGAAATGGT 
GCCCTGCTTG GAAACCATGT CAATCATGTT AATAGTGATA GGAGAGATTC CCTTCAGCAG 
ACCAATACCA CCCACCGTCC CCTGCATGTC CAAAGGCCTT CAATTCCACC TGCAAGTGAT 
ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA AATTCGGTGT GTCATAACCA TCATAACCAT 
AATTCCATAG GAAAGCAAGT TCCCACCTCA ACAAATGCCA ATCTCAATAA TGCCAATATG 
TCCAAAGCTG CCCATGGAAA GCGGCCCAGC ATTGGGAACC TTGAGCATGT GTCTGAAAAT 
GGGCATCATT CTTCCCACAA GCATGACCGG GAGCCTCAGA GAAGGTCCAG TGTGAAAAGA 
ACCCGCTATT ATGAAACTTA CATTAGGTCC GACTCAGGAG ATGAACAGCT CCCAACTATT 
TGCCGGGAAG ACCCAGAGAT ACATGGCTAT TTCAGGGACC CCCACTGCTT GGGGGAGCAG 
GAGTATTTCA GTAGTGAGGA ATGCTACGAG GATGACAGCT CGCCCACCTG GAGCAGGCAA 
AACTATGGCT ACTACAGCAG ATACCCAGGC AGAAACATCG ACTCTGAGAG GCCCCGAGGC 
TACCATCATC CCCAAGGATT CTTGGAGGAC GATGACTCGC CCGTTTGCTA TGATTCACGG 
AGATCTCCAA GGAGACGCCT ACTACCTCCC ACCCCAGCAT CCCACCGGAG ATCCTCCTTC 
AACTTTGAGT GCCTGCGCCG GCAGAGCAGC CAGGAAGAGG TCCCGTCGTC TCCCATCTTC 
CCCCATCGCA CGGCCCTGCC TCTGCATCTA ATGCAGCAAC AGATCATGGC AGTTGCCGGC 
CTAGATTCAA GTAAAGCCCA GAAGTACTCA CCGAGTCACT CGACCCGGTC GTGGGCCACC 
CCTCCAGCAA CCCCTCCCTA CCGGGACTGG ACACCGTGCT ACACCCCCCT GATCCAAGTG 
GAGCAGTCAG AGGCCCTGGA CCAGGTGAAC GGCAGCCTGC CGTCCCTGCA CCGCAGCTCC 
TGGTACACAG ACGAGCCCGA CATCTCCTAC CGGACTTTCA CACCAGCCAG CCTGACTGTC 
CCCAGCAGCT TCCGGAACAA AAACAGCGAC AAGCAGAGGA GTGCGGACAG CTTGGTGGAG 
GCAGTCCTGA TATCCGAAGG CTTGGGACGC TATGCAAGGG ACCCAAAATT TGTGTCAGCA 
ACAAAACACG AAATCGCTGA TGCCTGTGAC CTCACCATCG ACGAGATGGA GAGTGCAGCC 
AGCACCCTGC TTAATGGGAA CGTGCGTCCC CGAGCCAACG GGGATGTGGG CCCCCTCTCA 
CACCGGCAGG ACTATGAGCT ACAGGACTTT GGTCCTGGCT ACAGCGACGA AGAGCCAGAC 
CCTGGGAGGG ATGAGGAGGA CCTGGCGGAT GAAATGATAT GCATCACCAC CTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862633.1
CDS	16..6672
Translation	MPGEPLPLWGSPPQLWPGHHVQLPRRPGLGRGRMEQLLASPPLWRPPVPPNPCRLEAFPEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGENRGCCGSLWCWWRRRGAAKAGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENIGPIYNHRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKHYFTDAWNTFDALIVVGSVVDIAITEVNPTESENVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEDDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGNLEHVSENGHHSSHKHDREPQRRSSVKRTRYYETYIRSDSGDEQLPTICREDPEIHGYFRDPHCLGEQEYFSSEECYEDDSSPTWSRQNYGYYSRYPGRNIDSERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPASHRRSSFNFECLRRQSSQEEVPSSPIFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVEQSEALDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGNVRPRANGDVGPLSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017007144.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007144.1

ORF Insert Sequence:

ATGCCCGGGG AGCCGCTGCC GCTGTGGGGC TCCCCTCCCC AGCTCTGGCC CGGGCACCAC 
GTGCAGCTTC CACGCCGGCC GGGGCTGGGT CGAGGGAGGA TGGAGCAGCT CCTGGCCAGC 
CCTCCCCTTT GGAGACCGCC AGTGCCCCCC AACCCCTGTC GGCTGGAAGC CTTTCCGGAG 
GCAAACTATG CAAGAGGCAC CAGACTTCCT CTTTCTGGTG AAGGACCAAC TTCTCAGCCG 
AATAGCTCCA AGCAAACTGT CCTGTCTTGG CAAGCTGCAA TCGATGCTGC TAGACAGGCC 
AAGGCTGCCC AAACTATGAG CACCTCTGCA CCCCCACCTG TAGGATCTCT CTCCCAAAGA 
AAACGTCAGC AATACGCCAA GAGCAAAAAA CAGGGTAACT CGTCCAACAG CCGACCTGCC 
CGCGCCCTTT TCTGTTTATC ACTCAATAAC CCCATCCGAA GAGCCTGCAT TAGTATAGTG 
GAATGGAAAC CATTTGACAT ATTTATATTA TTGGCTATTT TTGCCAATTG TGTGGCCTTA 
GCTATTTACA TCCCATTCCC TGAAGATGAT TCTAATTCAA CAAATCATAA CTTGGAAAAA 
GTAGAATATG CCTTCCTGAT TATTTTTACA GTCGAGACAT TTTTGAAGAT TATAGCGTAT 
GGATTATTGC TACATCCTAA TGCTTATGTT AGGAATGGAT GGAATTTACT GGATTTTGTT 
ATAGTAATAG TAGGATTGTT TAGTGTAATT TTGGAACAAT TAACCAAAGA AACAGAAGGC 
GGGAACCACT CAAGCGGCAA ATCTGGAGGC TTTGATGTCA AAGCCCTCCG TGCCTTTCGA 
GTGTTGCGAC CACTTCGACT AGTGTCAGGA GTGCCCAGTT TACAAGTTGT CCTGAACTCC 
ATTATAAAAG CCATGGTTCC CCTCCTTCAC ATAGCCCTTT TGGTATTATT TGTAATCATA 
ATCTATGCTA TTATAGGATT GGAACTTTTT ATTGGAAAAA TGCACAAAAC ATGTTTTTTT 
GCTGACTCAG ATATCGTAGC TGAAGAGGAC CCAGCTCCAT GTGCGTTCTC AGGGAATGGA 
CGCCAGTGTA CTGCCAATGG CACGGAATGT AGGAGTGGCT GGGTTGGCCC GAACGGAGGC 
ATCACCAACT TTGATAACTT TGCCTTTGCC ATGCTTACTG TGTTTCAGTG CATCACCATG 
GAGGGCTGGA CAGATGTGCT CTACTGGATG AATGATGCTA TGGGATTTGA ATTGCCCTGG 
GTGTATTTTG TCAGTCTCGT CATCTTTGGG TCATTTTTCG TACTAAATCT TGTACTTGGT 
GTATTGAGCG GAGAATTCTC AAAGGAAAGA GAGAAGGCAA AAGCACGGGG AGATTTCCAG 
AAGCTCCGGG AGAAGCAGCA GCTGGAGGAG GATCTAAAGG GCTACTTGGA TTGGATCACC 
CAAGCTGAGG ACATCGATCC GGAGAATGAG GAAGAAGGAG GAGAGGAAGG CAAACGAAAT 
ACTAGCATGC CCACCAGCGA GACTGAGTCT GTGAACACAG AGAACGTCAG CGGTGAAGGC 
GAGAACCGAG GCTGCTGTGG AAGTCTCTGG TGCTGGTGGA GACGGAGAGG CGCGGCCAAG 
GCGGGGCCCT CTGGGTGTCG GCGGTGGGGT CAAGCCATCT CAAAATCCAA ACTCAGCCGA 
CGCTGGCGTC GCTGGAACCG ATTCAATCGC AGAAGATGTA GGGCCGCCGT GAAGTCTGTC 
ACGTTTTACT GGCTGGTTAT CGTCCTGGTG TTTCTGAACA CCTTAACCAT TTCCTCTGAG 
CACTACAATC AGCCAGATTG GTTGACACAG ATTCAAGATA TTGCCAACAA AGTCCTCTTG 
GCTCTGTTCA CCTGCGAGAT GCTGGTAAAA ATGTACAGCT TGGGCCTCCA AGCATATTTC 
GTCTCTCTTT TCAACCGGTT TGATTGCTTC GTGGTGTGTG GTGGAATCAC TGAGACGATC 
TTGGTGGAAC TGGAAATCAT GTCTCCCCTG GGGATCTCTG TGTTTCGGTG TGTGCGCCTC 
TTAAGAATCT TCAAAGTGAC CAGGCACTGG ACTTCCCTGA GCAACTTAGT GGCATCCTTA 
TTAAACTCCA TGAAGTCCAT CGCTTCGCTG TTGCTTCTGC TTTTTCTCTT CATTATCATC 
TTTTCCTTGC TTGGGATGCA GCTGTTTGGC GGCAAGTTTA ATTTTGATGA AACGCAAACC 
AAGCGGAGCA CCTTTGACAA TTTCCCTCAA GCACTTCTCA CAGTGTTCCA GATCCTGACA 
GGCGAAGACT GGAATGCTGT GATGTACGAT GGCATCATGG CTTACGGGGG CCCATCCTCT 
TCAGGAATGA TCGTCTGCAT CTACTTCATC ATCCTCTTCA TTTGTGGTAA CTATATTCTA 
CTGAATGTCT TCTTGGCCAT CGCTGTAGAC AATTTGGCTG ATGCTGAAAG TCTGAACACT 
GCTCAGAAAG AAGAAGCGGA AGAAAAGGAG AGGAAAAAGA TTGCCAGAAA AGAGAGCCTA 
GAAAATAAAA AGAACAACAA ACCAGAAGTC AACCAGATAG CCAACAGTGA CAACAAGGTT 
ACAATTGATG ACTATAGAGA AGAGGATGAA GACAAGGACC CCTATCCGCC TTGCGATGTG 
CCAGTAGGGG AAGAGGAAGA GGAAGAGGAG GAGGATGAAC CTGAGGTTCC TGCCGGACCC 
CGTCCTCGAA GGATCTCGGA GTTGAACATG AAGGAAAAAA TTGCCCCCAT CCCTGAAGGG 
AGCGCTTTCT TCATTCTTAG CAAGACCAAC CCGATCCGCG TAGGCTGCCA CAAGCTCATC 
AACCACCACA TCTTCACCAA CCTCATCCTT GTCTTCATCA TGCTGAGCAG CGCTGCCCTG 
GCCGCAGAGG ACCCCATCCG CAGCCACTCC TTCCGGAACA CGATACTGGG TTACTTTGAC 
TATGCCTTCA CAGCCATCTT TACTGTTGAG ATCCTGTTGA AGATGACAAC TTTTGGAGCT 
TTCCTCCACA AAGGGGCCTT CTGCAGGAAC TACTTCAATT TGCTGGATAT GCTGGTGGTT 
GGGGTGTCTC TGGTGTCATT TGGGATTCAA TCCAGTGCCA TCTCCGTTGT GAAGATTCTG 
AGGGTCTTAA GGGTCCTGCG TCCCCTCAGG GCCATCAACA GAGCAAAAGG ACTTAAGCAC 
GTGGTCCAGT GCGTCTTCGT GGCCATCCGG ACCATCGGCA ACATCATGAT CGTCACCACC 
CTCCTGCAGT TCATGTTTGC CTGTATCGGG GTCCAGTTGT TCAAGGGGAA GTTCTATCGC 
TGTACGGATG AAGCCAAAAG TAACCCTGAA GAATGCAGGG GACTTTTCAT CCTCTACAAG 
GATGGGGATG TTGACAGTCC TGTGGTCCGT GAACGGATCT GGCAAAACAG TGATTTCAAC 
TTCGACAACG TCCTCTCTGC TATGATGGCG CTCTTCACAG TCTCCACGTT TGAGGGCTGG 
CCTGCGTTGC TGTATAAAGC CATCGACTCG AATGGAGAGA ACATCGGCCC AATCTACAAC 
CACCGCGTGG AGATCTCCAT CTTCTTCATC ATCTACATCA TCATTGTAGC TTTCTTCATG 
ATGAACATCT TTGTGGGCTT TGTCATCGTT ACATTTCAGG AACAAGGAGA AAAAGAGTAT 
AAGAACTGTG AGCTGGACAA AAATCAGCGT CAGTGTGTTG AATACGCCTT GAAAGCACGT 
CCCTTGCGGA GATACATCCC CAAAAACCCC TACCAGTACA AGTTCTGGTA CGTGGTGAAC 
TCTTCGCCTT TCGAATACAT GATGTTTGTC CTCATCATGC TCAACACACT CTGCTTGGCC 
ATGCAGCACT ACGAGCAGTC CAAGATGTTC AATGATGCCA TGGACATTCT GAACATGGTC 
TTCACCGGGG TGTTCACCGT CGAGATGGTT TTGAAAGTCA TCGCATTTAA GCCTAAGCAC 
TATTTCACTG ATGCATGGAA CACTTTTGAT GCCTTAATTG TTGTTGGTAG CGTCGTTGAT 
ATTGCTATAA CTGAAGTGAA TCCAACTGAA AGTGAAAATG TCCCTGTCCC AACTGCTACA 
CCTGGGAACT CTGAAGAGAG CAATAGAATC TCCATCACCT TTTTCCGTCT TTTCCGAGTG 
ATGCGATTGG TGAAGCTTCT CAGCAGGGGG GAAGGCATCC GGACATTGCT GTGGACTTTT 
ATTAAGTCCT TTCAGGCGCT CCCGTATGTG GCCCTCCTCA TAGCCATGCT GTTCTTCATC 
TATGCGGTCA TTGGCATGCA GATGTTTGGG AAAGTTGCCA TGAGAGATAA CAACCAGATC 
AATAGGAACA ATAACTTCCA GACGTTTCCC CAGGCGGTGC TGCTGCTCTT CAGGTGTGCA 
ACAGGTGAGG CCTGGCAGGA GATCATGCTG GCCTGTCTCC CAGGGAAGCT CTGTGACCCT 
GAGTCAGATT ACAACCCCGG GGAGGAGTAT ACATGTGGGA GCAACTTTGC CATTGTCTAT 
TTCATCAGTT TTTACATGCT CTGTGCATTT CTGATCATCA ATCTGTTTGT GGCTGTCATC 
ATGGATAATT TCGACTATCT GACCCGGGAC TGGTCTATTT TGGGGCCTCA CCATTTAGAT 
GAATTCAAAA GAATATGGTC AGAATATGAC CCTGAGGCAA AGGGAAGGAT AAAACACCTT 
GATGTGGTCA CTCTGCTTCG ACGCATCCAG CCTCCCCTGG GGTTTGGGAA GTTATGTCCA 
CACAGGGTAG CGTGCAAGAG ATTAGTTGCC ATGAACATGC CTCTCAACAG TGACGGGACA 
GTCATGTTTA ATGCAACCCT GTTTGCTTTG GTTCGAACGG CTCTTAAGAT CAAGACCGAA 
GGGAACCTGG AGCAAGCTAA TGAAGAACTT CGGGCTGTGA TAAAGAAAAT TTGGAAGAAA 
ACCAGCATGA AATTACTTGA CCAAGTTGTC CCTCCAGCTG GTGATGATGA GGTAACCGTG 
GGGAAGTTCT ATGCCACTTT CCTGATACAG GACTACTTTA GGAAATTCAA GAAACGGAAA 
GAACAAGGAC TGGTGGGAAA GTACCCTGCG AAGAACACCA CAATTGCCCT ACAGGCGGGA 
TTAAGGACAC TGCATGACAT TGGGCCAGAA ATCCGGCGTG CTATATCGTG TGATTTGCAA 
GATGACGAGC CTGAGGAAAC AAAACGAGAA GAAGAAGATG ATGTGTTCAA AAGAAATGGT 
GCCCTGCTTG GAAACCATGT CAATCATGTT AATAGTGATA GGAGAGATTC CCTTCAGCAG 
ACCAATACCA CCCACCGTCC CCTGCATGTC CAAAGGCCTT CAATTCCACC TGCAAGTGAT 
ACTGAGAAAC CGCTGTTTCC TCCAGCAGGA AATTCGGTGT GTCATAACCA TCATAACCAT 
AATTCCATAG GAAAGCAAGT TCCCACCTCA ACAAATGCCA ATCTCAATAA TGCCAATATG 
TCCAAAGCTG CCCATGGAAA GCGGCCCAGC ATTGGGAACC TTGAGCATGT GTCTGAAAAT 
GGGCATCATT CTTCCCACAA GCATGACCGG GAGCCTCAGA GAAGGTCCAG TGTGAAAAGA 
ACCCGCTATT ATGAAACTTA CATTAGGTCC GACTCAGGAG ATGAACAGCT CCCAACTATT 
TGCCGGGAAG ACCCAGAGAT ACATGGCTAT TTCAGGGACC CCCACTGCTT GGGGGAGCAG 
GAGTATTTCA GTAGTGAGGA ATGCTACGAG GATGACAGCT CGCCCACCTG GAGCAGGCAA 
AACTATGGCT ACTACAGCAG ATACCCAGGC AGAAACATCG ACTCTGAGAG GCCCCGAGGC 
TACCATCATC CCCAAGGATT CTTGGAGGAC GATGACTCGC CCGTTTGCTA TGATTCACGG 
AGATCTCCAA GGAGACGCCT ACTACCTCCC ACCCCAGCAT CCCACCGGAG ATCCTCCTTC 
AACTTTGAGT GCCTGCGCCG GCAGAGCAGC CAGGAAGAGG TCCCGTCGTC TCCCATCTTC 
CCCCATCGCA CGGCCCTGCC TCTGCATCTA ATGCAGCAAC AGATCATGGC AGTTGCCGGC 
CTAGATTCAA GTAAAGCCCA GAAGTACTCA CCGAGTCACT CGACCCGGTC GTGGGCCACC 
CCTCCAGCAA CCCCTCCCTA CCGGGACTGG ACACCGTGCT ACACCCCCCT GATCCAAGTG 
GAGCAGTCAG AGGCCCTGGA CCAGGTGAAC GGCAGCCTGC CGTCCCTGCA CCGCAGCTCC 
TGGTACACAG ACGAGCCCGA CATCTCCTAC CGGACTTTCA CACCAGCCAG CCTGACTGTC 
CCCAGCAGCT TCCGGAACAA AAACAGCGAC AAGCAGAGGA GTGCGGACAG CTTGGTGGAG 
GCAGTCCTGA TATCCGAAGG CTTGGGACGC TATGCAAGGG ACCCAAAATT TGTGTCAGCA 
ACAAAACACG AAATCGCTGA TGCCTGTGAC CTCACCATCG ACGAGATGGA GAGTGCAGCC 
AGCACCCTGC TTAATGGGAA CGTGCGTCCC CGAGCCAACG GGGATGTGGG CCCCCTCTCA 
CACCGGCAGG ACTATGAGCT ACAGGACTTT GGTCCTGGCT ACAGCGACGA AGAGCCAGAC 
CCTGGGAGGG ATGAGGAGGA CCTGGCGGAT GAAATGATAT GCATCACCAC CTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_017007145.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862634.1 Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X10, mRNA.

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CloneID	OHu94547
Clone ID Related Accession (Same CDS sequence)	XM_017007145.1
Accession Version	XM_017007145.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGGG AGCCGCTGCC GCTGTGGGGC TCCCCTCCCC AGCTCTGGCC CGGGCACCAC 
GTGCAGCTTC CACGCCGGCC GGGGCTGGGT CGAGGGAGGA TGGAGCAGCT CCTGGCCAGC 
CCTCCCCTTT GGAGACCGCC AGTGCCCCCC AACCCCTGTC GGCTGGAAGC CTTTCCGGAG 
GCAAACTATG CAAGAGGCAC CAGACTTCCT CTTTCTGGTG AAGGACCAAC TTCTCAGCCG 
AATAGCTCCA AGCAAACTGT CCTGTCTTGG CAAGCTGCAA TCGATGCTGC TAGACAGGCC 
AAGGCTGCCC AAACTATGAG CACCTCTGCA CCCCCACCTG TAGGATCTCT CTCCCAAAGA 
AAACGTCAGC AATACGCCAA GAGCAAAAAA CAGGGTAACT CGTCCAACAG CCGACCTGCC 
CGCGCCCTTT TCTGTTTATC ACTCAATAAC CCCATCCGAA GAGCCTGCAT TAGTATAGTG 
GAATGGAAAC CATTTGACAT ATTTATATTA TTGGCTATTT TTGCCAATTG TGTGGCCTTA 
GCTATTTACA TCCCATTCCC TGAAGATGAT TCTAATTCAA CAAATCATAA CTTGGAAAAA 
GTAGAATATG CCTTCCTGAT TATTTTTACA GTCGAGACAT TTTTGAAGAT TATAGCGTAT 
GGATTATTGC TACATCCTAA TGCTTATGTT AGGAATGGAT GGAATTTACT GGATTTTGTT 
ATAGTAATAG TAGGATTGTT TAGTGTAATT TTGGAACAAT TAACCAAAGA AACAGAAGGC 
GGGAACCACT CAAGCGGCAA ATCTGGAGGC TTTGATGTCA AAGCCCTCCG TGCCTTTCGA 
GTGTTGCGAC CACTTCGACT AGTGTCAGGA GTGCCCAGTT TACAAGTTGT CCTGAACTCC 
ATTATAAAAG CCATGGTTCC CCTCCTTCAC ATAGCCCTTT TGGTATTATT TGTAATCATA 
ATCTATGCTA TTATAGGATT GGAACTTTTT ATTGGAAAAA TGCACAAAAC ATGTTTTTTT 
GCTGACTCAG ATATCGTAGC TGAAGAGGAC CCAGCTCCAT GTGCGTTCTC AGGGAATGGA 
CGCCAGTGTA CTGCCAATGG CACGGAATGT AGGAGTGGCT GGGTTGGCCC GAACGGAGGC 
ATCACCAACT TTGATAACTT TGCCTTTGCC ATGCTTACTG TGTTTCAGTG CATCACCATG 
GAGGGCTGGA CAGATGTGCT CTACTGGGTA AATGATGCGA TAGGATGGGA ATGGCCATGG 
GTGTATTTTG TTAGTCTGAT CATCCTTGGC TCATTTTTCG TCCTTAACCT GGTTCTTGGT 
GTCCTTAGTG GAGAATTCTC AAAGGAAAGA GAGAAGGCAA AAGCACGGGG AGATTTCCAG 
AAGCTCCGGG AGAAGCAGCA GCTGGAGGAG GATCTAAAGG GCTACTTGGA TTGGATCACC 
CAAGCTGAGG ACATCGATCC GGAGAATGAG GAAGAAGGAG GAGAGGAAGG CAAACGAAAT 
ACTAGCATGC CCACCAGCGA GACTGAGTCT GTGAACACAG AGAACGTCAG CGGTGAAGGC 
GAGAACCGAG GCTGCTGTGG AAGTCTCTGG TGCTGGTGGA GACGGAGAGG CGCGGCCAAG 
GCGGGGCCCT CTGGGTGTCG GCGGTGGGGT CAAGCCATCT CAAAATCCAA ACTCAGCCGA 
CGCTGGCGTC GCTGGAACCG ATTCAATCGC AGAAGATGTA GGGCCGCCGT GAAGTCTGTC 
ACGTTTTACT GGCTGGTTAT CGTCCTGGTG TTTCTGAACA CCTTAACCAT TTCCTCTGAG 
CACTACAATC AGCCAGATTG GTTGACACAG ATTCAAGATA TTGCCAACAA AGTCCTCTTG 
GCTCTGTTCA CCTGCGAGAT GCTGGTAAAA ATGTACAGCT TGGGCCTCCA AGCATATTTC 
GTCTCTCTTT TCAACCGGTT TGATTGCTTC GTGGTGTGTG GTGGAATCAC TGAGACGATC 
TTGGTGGAAC TGGAAATCAT GTCTCCCCTG GGGATCTCTG TGTTTCGGTG TGTGCGCCTC 
TTAAGAATCT TCAAAGTGAC CAGGCACTGG ACTTCCCTGA GCAACTTAGT GGCATCCTTA 
TTAAACTCCA TGAAGTCCAT CGCTTCGCTG TTGCTTCTGC TTTTTCTCTT CATTATCATC 
TTTTCCTTGC TTGGGATGCA GCTGTTTGGC GGCAAGTTTA ATTTTGATGA AACGCAAACC 
AAGCGGAGCA CCTTTGACAA TTTCCCTCAA GCACTTCTCA CAGTGTTCCA GATCCTGACA 
GGCGAAGACT GGAATGCTGT GATGTACGAT GGCATCATGG CTTACGGGGG CCCATCCTCT 
TCAGGAATGA TCGTCTGCAT CTACTTCATC ATCCTCTTCA TTTGTGGTAA CTATATTCTA 
CTGAATGTCT TCTTGGCCAT CGCTGTAGAC AATTTGGCTG ATGCTGAAAG TCTGAACACT 
GCTCAGAAAG AAGAAGCGGA AGAAAAGGAG AGGAAAAAGA TTGCCAGAAA AGAGAGCCTA 
GAAAATAAAA AGAACAACAA ACCAGAAGTC AACCAGATAG CCAACAGTGA CAACAAGGTT 
ACAATTGATG ACTATAGAGA AGAGGATGAA GACAAGGACC CCTATCCGCC TTGCGATGTG 
CCAGTAGGGG AAGAGGAAGA GGAAGAGGAG GAGGATGAAC CTGAGGTTCC TGCCGGACCC 
CGTCCTCGAA GGATCTCGGA GTTGAACATG AAGGAAAAAA TTGCCCCCAT CCCTGAAGGG 
AGCGCTTTCT TCATTCTTAG CAAGACCAAC CCGATCCGCG TAGGCTGCCA CAAGCTCATC 
AACCACCACA TCTTCACCAA CCTCATCCTT GTCTTCATCA TGCTGAGCAG CGCTGCCCTG 
GCCGCAGAGG ACCCCATCCG CAGCCACTCC TTCCGGAACA CGATACTGGG TTACTTTGAC 
TATGCCTTCA CAGCCATCTT TACTGTTGAG ATCCTGTTGA AGATGACAAC TTTTGGAGCT 
TTCCTCCACA AAGGGGCCTT CTGCAGGAAC TACTTCAATT TGCTGGATAT GCTGGTGGTT 
GGGGTGTCTC TGGTGTCATT TGGGATTCAA TCCAGTGCCA TCTCCGTTGT GAAGATTCTG 
AGGGTCTTAA GGGTCCTGCG TCCCCTCAGG GCCATCAACA GAGCAAAAGG ACTTAAGCAC 
GTGGTCCAGT GCGTCTTCGT GGCCATCCGG ACCATCGGCA ACATCATGAT CGTCACCACC 
CTCCTGCAGT TCATGTTTGC CTGTATCGGG GTCCAGTTGT TCAAGGGGAA GTTCTATCGC 
TGTACGGATG AAGCCAAAAG TAACCCTGAA GAATGCAGGG GACTTTTCAT CCTCTACAAG 
GATGGGGATG TTGACAGTCC TGTGGTCCGT GAACGGATCT GGCAAAACAG TGATTTCAAC 
TTCGACAACG TCCTCTCTGC TATGATGGCG CTCTTCACAG TCTCCACGTT TGAGGGCTGG 
CCTGCGTTGC TGTATAAAGC CATCGACTCG AATGGAGAGA ACATCGGCCC AATCTACAAC 
CACCGCGTGG AGATCTCCAT CTTCTTCATC ATCTACATCA TCATTGTAGC TTTCTTCATG 
ATGAACATCT TTGTGGGCTT TGTCATCGTT ACATTTCAGG AACAAGGAGA AAAAGAGTAT 
AAGAACTGTG AGCTGGACAA AAATCAGCGT CAGTGTGTTG AATACGCCTT GAAAGCACGT 
CCCTTGCGGA GATACATCCC CAAAAACCCC TACCAGTACA AGTTCTGGTA CGTGGTGAAC 
TCTTCGCCTT TCGAATACAT GATGTTTGTC CTCATCATGC TCAACACACT CTGCTTGGCC 
ATGCAGCACT ACGAGCAGTC CAAGATGTTC AATGATGCCA TGGACATTCT GAACATGGTC 
TTCACCGGGG TGTTCACCGT CGAGATGGTT TTGAAAGTCA TCGCATTTAA GCCTAAGGGG 
TATTTTAGTG ACGCCTGGAA CACGTTTGAC TCCCTCATCG TAATCGGCAG CATTATAGAC 
GTGGCCCTCA GCGAAGCAGA CAACTCTGAA GAGAGCAATA GAATCTCCAT CACCTTTTTC 
CGTCTTTTCC GAGTGATGCG ATTGGTGAAG CTTCTCAGCA GGGGGGAAGG CATCCGGACA 
TTGCTGTGGA CTTTTATTAA GTCCTTTCAG GCGCTCCCGT ATGTGGCCCT CCTCATAGCC 
ATGCTGTTCT TCATCTATGC GGTCATTGGC ATGCAGATGT TTGGGAAAGT TGCCATGAGA 
GATAACAACC AGATCAATAG GAACAATAAC TTCCAGACGT TTCCCCAGGC GGTGCTGCTG 
CTCTTCAGGT GTGCAACAGG TGAGGCCTGG CAGGAGATCA TGCTGGCCTG TCTCCCAGGG 
AAGCTCTGTG ACCCTGAGTC AGATTACAAC CCCGGGGAGG AGTATACATG TGGGAGCAAC 
TTTGCCATTG TCTATTTCAT CAGTTTTTAC ATGCTCTGTG CATTTCTGAT CATCAATCTG 
TTTGTGGCTG TCATCATGGA TAATTTCGAC TATCTGACCC GGGACTGGTC TATTTTGGGG 
CCTCACCATT TAGATGAATT CAAAAGAATA TGGTCAGAAT ATGACCCTGA GGCAAAGGGA 
AGGATAAAAC ACCTTGATGT GGTCACTCTG CTTCGACGCA TCCAGCCTCC CCTGGGGTTT 
GGGAAGTTAT GTCCACACAG GGTAGCGTGC AAGAGATTAG TTGCCATGAA CATGCCTCTC 
AACAGTGACG GGACAGTCAT GTTTAATGCA ACCCTGTTTG CTTTGGTTCG AACGGCTCTT 
AAGATCAAGA CCGAAGGGAA CCTGGAGCAA GCTAATGAAG AACTTCGGGC TGTGATAAAG 
AAAATTTGGA AGAAAACCAG CATGAAATTA CTTGACCAAG TTGTCCCTCC AGCTGGTGAT 
GATGAGGTAA CCGTGGGGAA GTTCTATGCC ACTTTCCTGA TACAGGACTA CTTTAGGAAA 
TTCAAGAAAC GGAAAGAACA AGGACTGGTG GGAAAGTACC CTGCGAAGAA CACCACAATT 
GCCCTACAGG CGGGATTAAG GACACTGCAT GACATTGGGC CAGAAATCCG GCGTGCTATA 
TCGTGTGATT TGCAAGATGA CGAGCCTGAG GAAACAAAAC GAGAAGAAGA AGATGATGTG 
TTCAAAAGAA ATGGTGCCCT GCTTGGAAAC CATGTCAATC ATGTTAATAG TGATAGGAGA 
GATTCCCTTC AGCAGACCAA TACCACCCAC CGTCCCCTGC ATGTCCAAAG GCCTTCAATT 
CCACCTGCAA GTGATACTGA GAAACCGCTG TTTCCTCCAG CAGGAAATTC GGTGTGTCAT 
AACCATCATA ACCATAATTC CATAGGAAAG CAAGTTCCCA CCTCAACAAA TGCCAATCTC 
AATAATGCCA ATATGTCCAA AGCTGCCCAT GGAAAGCGGC CCAGCATTGG GAACCTTGAG 
CATGTGTCTG AAAATGGGCA TCATTCTTCC CACAAGCATG ACCGGGAGCC TCAGAGAAGG 
TCCAGTGTGA AAAGAACCCG CTATTATGAA ACTTACATTA GGTCCGACTC AGGAGATGAA 
CAGCTCCCAA CTATTTGCCG GGAAGACCCA GAGATACATG GCTATTTCAG GGACCCCCAC 
TGCTTGGGGG AGCAGGAGTA TTTCAGTAGT GAGGAATGCT ACGAGGATGA CAGCTCGCCC 
ACCTGGAGCA GGCAAAACTA TGGCTACTAC AGCAGATACC CAGGCAGAAA CATCGACTCT 
GAGAGGCCCC GAGGCTACCA TCATCCCCAA GGATTCTTGG AGGACGATGA CTCGCCCGTT 
TGCTATGATT CACGGAGATC TCCAAGGAGA CGCCTACTAC CTCCCACCCC AGCATCCCAC 
CGGAGATCCT CCTTCAACTT TGAGTGCCTG CGCCGGCAGA GCAGCCAGGA AGAGGTCCCG 
TCGTCTCCCA TCTTCCCCCA TCGCACGGCC CTGCCTCTGC ATCTAATGCA GCAACAGATC 
ATGGCAGTTG CCGGCCTAGA TTCAAGTAAA GCCCAGAAGT ACTCACCGAG TCACTCGACC 
CGGTCGTGGG CCACCCCTCC AGCAACCCCT CCCTACCGGG ACTGGACACC GTGCTACACC 
CCCCTGATCC AAGTGGAGCA GTCAGAGGCC CTGGACCAGG TGAACGGCAG CCTGCCGTCC 
CTGCACCGCA GCTCCTGGTA CACAGACGAG CCCGACATCT CCTACCGGAC TTTCACACCA 
GCCAGCCTGA CTGTCCCCAG CAGCTTCCGG AACAAAAACA GCGACAAGCA GAGGAGTGCG 
GACAGCTTGG TGGAGGCAGT CCTGATATCC GAAGGCTTGG GACGCTATGC AAGGGACCCA 
AAATTTGTGT CAGCAACAAA ACACGAAATC GCTGATGCCT GTGACCTCAC CATCGACGAG 
ATGGAGAGTG CAGCCAGCAC CCTGCTTAAT GGGAACGTGC GTCCCCGAGC CAACGGGGAT 
GTGGGCCCCC TCTCACACCG GCAGGACTAT GAGCTACAGG ACTTTGGTCC TGGCTACAGC 
GACGAAGAGC CAGACCCTGG GAGGGATGAG GAGGACCTGG CGGATGAAAT GATATGCATC 
ACCACCTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862634.1
CDS	16..6627
Translation	MPGEPLPLWGSPPQLWPGHHVQLPRRPGLGRGRMEQLLASPPLWRPPVPPNPCRLEAFPEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGENRGCCGSLWCWWRRRGAAKAGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENIGPIYNHRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEDDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGNLEHVSENGHHSSHKHDREPQRRSSVKRTRYYETYIRSDSGDEQLPTICREDPEIHGYFRDPHCLGEQEYFSSEECYEDDSSPTWSRQNYGYYSRYPGRNIDSERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPASHRRSSFNFECLRRQSSQEEVPSSPIFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVEQSEALDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGNVRPRANGDVGPLSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_017007145.1
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007145.1

ORF Insert Sequence:

ATGCCCGGGG AGCCGCTGCC GCTGTGGGGC TCCCCTCCCC AGCTCTGGCC CGGGCACCAC 
GTGCAGCTTC CACGCCGGCC GGGGCTGGGT CGAGGGAGGA TGGAGCAGCT CCTGGCCAGC 
CCTCCCCTTT GGAGACCGCC AGTGCCCCCC AACCCCTGTC GGCTGGAAGC CTTTCCGGAG 
GCAAACTATG CAAGAGGCAC CAGACTTCCT CTTTCTGGTG AAGGACCAAC TTCTCAGCCG 
AATAGCTCCA AGCAAACTGT CCTGTCTTGG CAAGCTGCAA TCGATGCTGC TAGACAGGCC 
AAGGCTGCCC AAACTATGAG CACCTCTGCA CCCCCACCTG TAGGATCTCT CTCCCAAAGA 
AAACGTCAGC AATACGCCAA GAGCAAAAAA CAGGGTAACT CGTCCAACAG CCGACCTGCC 
CGCGCCCTTT TCTGTTTATC ACTCAATAAC CCCATCCGAA GAGCCTGCAT TAGTATAGTG 
GAATGGAAAC CATTTGACAT ATTTATATTA TTGGCTATTT TTGCCAATTG TGTGGCCTTA 
GCTATTTACA TCCCATTCCC TGAAGATGAT TCTAATTCAA CAAATCATAA CTTGGAAAAA 
GTAGAATATG CCTTCCTGAT TATTTTTACA GTCGAGACAT TTTTGAAGAT TATAGCGTAT 
GGATTATTGC TACATCCTAA TGCTTATGTT AGGAATGGAT GGAATTTACT GGATTTTGTT 
ATAGTAATAG TAGGATTGTT TAGTGTAATT TTGGAACAAT TAACCAAAGA AACAGAAGGC 
GGGAACCACT CAAGCGGCAA ATCTGGAGGC TTTGATGTCA AAGCCCTCCG TGCCTTTCGA 
GTGTTGCGAC CACTTCGACT AGTGTCAGGA GTGCCCAGTT TACAAGTTGT CCTGAACTCC 
ATTATAAAAG CCATGGTTCC CCTCCTTCAC ATAGCCCTTT TGGTATTATT TGTAATCATA 
ATCTATGCTA TTATAGGATT GGAACTTTTT ATTGGAAAAA TGCACAAAAC ATGTTTTTTT 
GCTGACTCAG ATATCGTAGC TGAAGAGGAC CCAGCTCCAT GTGCGTTCTC AGGGAATGGA 
CGCCAGTGTA CTGCCAATGG CACGGAATGT AGGAGTGGCT GGGTTGGCCC GAACGGAGGC 
ATCACCAACT TTGATAACTT TGCCTTTGCC ATGCTTACTG TGTTTCAGTG CATCACCATG 
GAGGGCTGGA CAGATGTGCT CTACTGGGTA AATGATGCGA TAGGATGGGA ATGGCCATGG 
GTGTATTTTG TTAGTCTGAT CATCCTTGGC TCATTTTTCG TCCTTAACCT GGTTCTTGGT 
GTCCTTAGTG GAGAATTCTC AAAGGAAAGA GAGAAGGCAA AAGCACGGGG AGATTTCCAG 
AAGCTCCGGG AGAAGCAGCA GCTGGAGGAG GATCTAAAGG GCTACTTGGA TTGGATCACC 
CAAGCTGAGG ACATCGATCC GGAGAATGAG GAAGAAGGAG GAGAGGAAGG CAAACGAAAT 
ACTAGCATGC CCACCAGCGA GACTGAGTCT GTGAACACAG AGAACGTCAG CGGTGAAGGC 
GAGAACCGAG GCTGCTGTGG AAGTCTCTGG TGCTGGTGGA GACGGAGAGG CGCGGCCAAG 
GCGGGGCCCT CTGGGTGTCG GCGGTGGGGT CAAGCCATCT CAAAATCCAA ACTCAGCCGA 
CGCTGGCGTC GCTGGAACCG ATTCAATCGC AGAAGATGTA GGGCCGCCGT GAAGTCTGTC 
ACGTTTTACT GGCTGGTTAT CGTCCTGGTG TTTCTGAACA CCTTAACCAT TTCCTCTGAG 
CACTACAATC AGCCAGATTG GTTGACACAG ATTCAAGATA TTGCCAACAA AGTCCTCTTG 
GCTCTGTTCA CCTGCGAGAT GCTGGTAAAA ATGTACAGCT TGGGCCTCCA AGCATATTTC 
GTCTCTCTTT TCAACCGGTT TGATTGCTTC GTGGTGTGTG GTGGAATCAC TGAGACGATC 
TTGGTGGAAC TGGAAATCAT GTCTCCCCTG GGGATCTCTG TGTTTCGGTG TGTGCGCCTC 
TTAAGAATCT TCAAAGTGAC CAGGCACTGG ACTTCCCTGA GCAACTTAGT GGCATCCTTA 
TTAAACTCCA TGAAGTCCAT CGCTTCGCTG TTGCTTCTGC TTTTTCTCTT CATTATCATC 
TTTTCCTTGC TTGGGATGCA GCTGTTTGGC GGCAAGTTTA ATTTTGATGA AACGCAAACC 
AAGCGGAGCA CCTTTGACAA TTTCCCTCAA GCACTTCTCA CAGTGTTCCA GATCCTGACA 
GGCGAAGACT GGAATGCTGT GATGTACGAT GGCATCATGG CTTACGGGGG CCCATCCTCT 
TCAGGAATGA TCGTCTGCAT CTACTTCATC ATCCTCTTCA TTTGTGGTAA CTATATTCTA 
CTGAATGTCT TCTTGGCCAT CGCTGTAGAC AATTTGGCTG ATGCTGAAAG TCTGAACACT 
GCTCAGAAAG AAGAAGCGGA AGAAAAGGAG AGGAAAAAGA TTGCCAGAAA AGAGAGCCTA 
GAAAATAAAA AGAACAACAA ACCAGAAGTC AACCAGATAG CCAACAGTGA CAACAAGGTT 
ACAATTGATG ACTATAGAGA AGAGGATGAA GACAAGGACC CCTATCCGCC TTGCGATGTG 
CCAGTAGGGG AAGAGGAAGA GGAAGAGGAG GAGGATGAAC CTGAGGTTCC TGCCGGACCC 
CGTCCTCGAA GGATCTCGGA GTTGAACATG AAGGAAAAAA TTGCCCCCAT CCCTGAAGGG 
AGCGCTTTCT TCATTCTTAG CAAGACCAAC CCGATCCGCG TAGGCTGCCA CAAGCTCATC 
AACCACCACA TCTTCACCAA CCTCATCCTT GTCTTCATCA TGCTGAGCAG CGCTGCCCTG 
GCCGCAGAGG ACCCCATCCG CAGCCACTCC TTCCGGAACA CGATACTGGG TTACTTTGAC 
TATGCCTTCA CAGCCATCTT TACTGTTGAG ATCCTGTTGA AGATGACAAC TTTTGGAGCT 
TTCCTCCACA AAGGGGCCTT CTGCAGGAAC TACTTCAATT TGCTGGATAT GCTGGTGGTT 
GGGGTGTCTC TGGTGTCATT TGGGATTCAA TCCAGTGCCA TCTCCGTTGT GAAGATTCTG 
AGGGTCTTAA GGGTCCTGCG TCCCCTCAGG GCCATCAACA GAGCAAAAGG ACTTAAGCAC 
GTGGTCCAGT GCGTCTTCGT GGCCATCCGG ACCATCGGCA ACATCATGAT CGTCACCACC 
CTCCTGCAGT TCATGTTTGC CTGTATCGGG GTCCAGTTGT TCAAGGGGAA GTTCTATCGC 
TGTACGGATG AAGCCAAAAG TAACCCTGAA GAATGCAGGG GACTTTTCAT CCTCTACAAG 
GATGGGGATG TTGACAGTCC TGTGGTCCGT GAACGGATCT GGCAAAACAG TGATTTCAAC 
TTCGACAACG TCCTCTCTGC TATGATGGCG CTCTTCACAG TCTCCACGTT TGAGGGCTGG 
CCTGCGTTGC TGTATAAAGC CATCGACTCG AATGGAGAGA ACATCGGCCC AATCTACAAC 
CACCGCGTGG AGATCTCCAT CTTCTTCATC ATCTACATCA TCATTGTAGC TTTCTTCATG 
ATGAACATCT TTGTGGGCTT TGTCATCGTT ACATTTCAGG AACAAGGAGA AAAAGAGTAT 
AAGAACTGTG AGCTGGACAA AAATCAGCGT CAGTGTGTTG AATACGCCTT GAAAGCACGT 
CCCTTGCGGA GATACATCCC CAAAAACCCC TACCAGTACA AGTTCTGGTA CGTGGTGAAC 
TCTTCGCCTT TCGAATACAT GATGTTTGTC CTCATCATGC TCAACACACT CTGCTTGGCC 
ATGCAGCACT ACGAGCAGTC CAAGATGTTC AATGATGCCA TGGACATTCT GAACATGGTC 
TTCACCGGGG TGTTCACCGT CGAGATGGTT TTGAAAGTCA TCGCATTTAA GCCTAAGGGG 
TATTTTAGTG ACGCCTGGAA CACGTTTGAC TCCCTCATCG TAATCGGCAG CATTATAGAC 
GTGGCCCTCA GCGAAGCAGA CAACTCTGAA GAGAGCAATA GAATCTCCAT CACCTTTTTC 
CGTCTTTTCC GAGTGATGCG ATTGGTGAAG CTTCTCAGCA GGGGGGAAGG CATCCGGACA 
TTGCTGTGGA CTTTTATTAA GTCCTTTCAG GCGCTCCCGT ATGTGGCCCT CCTCATAGCC 
ATGCTGTTCT TCATCTATGC GGTCATTGGC ATGCAGATGT TTGGGAAAGT TGCCATGAGA 
GATAACAACC AGATCAATAG GAACAATAAC TTCCAGACGT TTCCCCAGGC GGTGCTGCTG 
CTCTTCAGGT GTGCAACAGG TGAGGCCTGG CAGGAGATCA TGCTGGCCTG TCTCCCAGGG 
AAGCTCTGTG ACCCTGAGTC AGATTACAAC CCCGGGGAGG AGTATACATG TGGGAGCAAC 
TTTGCCATTG TCTATTTCAT CAGTTTTTAC ATGCTCTGTG CATTTCTGAT CATCAATCTG 
TTTGTGGCTG TCATCATGGA TAATTTCGAC TATCTGACCC GGGACTGGTC TATTTTGGGG 
CCTCACCATT TAGATGAATT CAAAAGAATA TGGTCAGAAT ATGACCCTGA GGCAAAGGGA 
AGGATAAAAC ACCTTGATGT GGTCACTCTG CTTCGACGCA TCCAGCCTCC CCTGGGGTTT 
GGGAAGTTAT GTCCACACAG GGTAGCGTGC AAGAGATTAG TTGCCATGAA CATGCCTCTC 
AACAGTGACG GGACAGTCAT GTTTAATGCA ACCCTGTTTG CTTTGGTTCG AACGGCTCTT 
AAGATCAAGA CCGAAGGGAA CCTGGAGCAA GCTAATGAAG AACTTCGGGC TGTGATAAAG 
AAAATTTGGA AGAAAACCAG CATGAAATTA CTTGACCAAG TTGTCCCTCC AGCTGGTGAT 
GATGAGGTAA CCGTGGGGAA GTTCTATGCC ACTTTCCTGA TACAGGACTA CTTTAGGAAA 
TTCAAGAAAC GGAAAGAACA AGGACTGGTG GGAAAGTACC CTGCGAAGAA CACCACAATT 
GCCCTACAGG CGGGATTAAG GACACTGCAT GACATTGGGC CAGAAATCCG GCGTGCTATA 
TCGTGTGATT TGCAAGATGA CGAGCCTGAG GAAACAAAAC GAGAAGAAGA AGATGATGTG 
TTCAAAAGAA ATGGTGCCCT GCTTGGAAAC CATGTCAATC ATGTTAATAG TGATAGGAGA 
GATTCCCTTC AGCAGACCAA TACCACCCAC CGTCCCCTGC ATGTCCAAAG GCCTTCAATT 
CCACCTGCAA GTGATACTGA GAAACCGCTG TTTCCTCCAG CAGGAAATTC GGTGTGTCAT 
AACCATCATA ACCATAATTC CATAGGAAAG CAAGTTCCCA CCTCAACAAA TGCCAATCTC 
AATAATGCCA ATATGTCCAA AGCTGCCCAT GGAAAGCGGC CCAGCATTGG GAACCTTGAG 
CATGTGTCTG AAAATGGGCA TCATTCTTCC CACAAGCATG ACCGGGAGCC TCAGAGAAGG 
TCCAGTGTGA AAAGAACCCG CTATTATGAA ACTTACATTA GGTCCGACTC AGGAGATGAA 
CAGCTCCCAA CTATTTGCCG GGAAGACCCA GAGATACATG GCTATTTCAG GGACCCCCAC 
TGCTTGGGGG AGCAGGAGTA TTTCAGTAGT GAGGAATGCT ACGAGGATGA CAGCTCGCCC 
ACCTGGAGCA GGCAAAACTA TGGCTACTAC AGCAGATACC CAGGCAGAAA CATCGACTCT 
GAGAGGCCCC GAGGCTACCA TCATCCCCAA GGATTCTTGG AGGACGATGA CTCGCCCGTT 
TGCTATGATT CACGGAGATC TCCAAGGAGA CGCCTACTAC CTCCCACCCC AGCATCCCAC 
CGGAGATCCT CCTTCAACTT TGAGTGCCTG CGCCGGCAGA GCAGCCAGGA AGAGGTCCCG 
TCGTCTCCCA TCTTCCCCCA TCGCACGGCC CTGCCTCTGC ATCTAATGCA GCAACAGATC 
ATGGCAGTTG CCGGCCTAGA TTCAAGTAAA GCCCAGAAGT ACTCACCGAG TCACTCGACC 
CGGTCGTGGG CCACCCCTCC AGCAACCCCT CCCTACCGGG ACTGGACACC GTGCTACACC 
CCCCTGATCC AAGTGGAGCA GTCAGAGGCC CTGGACCAGG TGAACGGCAG CCTGCCGTCC 
CTGCACCGCA GCTCCTGGTA CACAGACGAG CCCGACATCT CCTACCGGAC TTTCACACCA 
GCCAGCCTGA CTGTCCCCAG CAGCTTCCGG AACAAAAACA GCGACAAGCA GAGGAGTGCG 
GACAGCTTGG TGGAGGCAGT CCTGATATCC GAAGGCTTGG GACGCTATGC AAGGGACCCA 
AAATTTGTGT CAGCAACAAA ACACGAAATC GCTGATGCCT GTGACCTCAC CATCGACGAG 
ATGGAGAGTG CAGCCAGCAC CCTGCTTAAT GGGAACGTGC GTCCCCGAGC CAACGGGGAT 
GTGGGCCCCC TCTCACACCG GCAGGACTAT GAGCTACAGG ACTTTGGTCC TGGCTACAGC 
GACGAAGAGC CAGACCCTGG GAGGGATGAG GAGGACCTGG CGGATGAAAT GATATGCATC 
ACCACCTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( NM_001128839.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001122311.1 Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant 3, mRNA.

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CloneID	OHu10032
Clone ID Related Accession (Same CDS sequence)	NM_001128839.2 , NM_001128839.3
Accession Version	NM_001128839.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M76558.1, M83566.1, EU363339.1, AC132810.2 and AW008794.1. On Dec 4, 2012 this sequence version replaced NM_001128839.1. Transcript Variant: This variant (3) includes alternate in-frame exons, compared to variant 1, resulting in a shorter protein (isoform c), compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU363339.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144120, SAMEA2145240 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTTCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACTGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTTTT CTGTTTATCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATAGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC AAGCGGCAAA TCTGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTTCGAG TGTTGCGACC ACTTCGACTA GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATCGTAGCT GAAGAGGACC CAGCTCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTAC TGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCG 
AACGGAGGCA TCACCAACTT TGATAACTTT GCCTTTGCCA TGCTTACTGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCAAA AGCACGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCTGAGGA CATCGATCCG GAGAATGAGG AAGAAGGAGG AGAGGAAGGC 
AAACGAAATA CTAGCATGCC CACCAGCGAG ACTGAGTCTG TGAACACAGA GAACGTCAGC 
GGTGAAGGCG AGAACCGAGG CTGCTGTGGA AGTCTCTGTC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGA TTCAATCGCA GAAGATGTAG GGCCGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATC GTCCTGGTGT TTCTGAACAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCAGATTGG TTGACACAGA TTCAAGATAT TGCCAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGCCTCCAA 
GCATATTTCG TCTCTCTTTT CAACCGGTTT GATTGCTTCG TGGTGTGTGG TGGAATCACT 
GAGACGATCT TGGTGGAACT GGAAATCATG TCTCCCCTGG GGATCTCTGT GTTTCGGTGT 
GTGCGCCTCT TAAGAATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCATCCTTAT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATTATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CACTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGACTG GAATGCTGTG ATGTACGATG GCATCATGGC TTACGGGGGC 
CCATCCTCTT CAGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA AGAAGCGGAA GAAAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTAG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA CTATAGAGAA GAGGATGAAG ACAAGGACCC CTATCCGCCT 
TGCGATGTGC CAGTAGGGGA AGAGGAAGAG GAAGAGGAGG AGGATGAACC TGAGGTTCCT 
GCCGGACCCC GTCCTCGAAG GATCTCGGAG TTGAACATGA AGGAAAAAAT TGCCCCCATC 
CCTGAAGGGA GCGCTTTCTT CATTCTTAGC AAGACCAACC CGATCCGCGT AGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC GATACTGGGT 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACTGTTGAGA TCCTGTTGAA GATGACAACT 
TTTGGAGCTT TCCTCCACAA AGGGGCCTTC TGCAGGAACT ACTTCAATTT GCTGGATATG 
CTGGTGGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGT CCCCTCAGGG CCATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG CGTCTTCGTG GCCATCCGGA CCATCGGCAA CATCATGATC 
GTCACCACCC TCCTGCAGTT CATGTTTGCC TGTATCGGGG TCCAGTTGTT CAAGGGGAAG 
TTCTATCGCT GTACGGATGA AGCCAAAAGT AACCCTGAAG AATGCAGGGG ACTTTTCATC 
CTCTACAAGG ATGGGGATGT TGACAGTCCT GTGGTCCGTG AACGGATCTG GCAAAACAGT 
GATTTCAACT TCGACAACGT CCTCTCTGCT ATGATGGCGC TCTTCACAGT CTCCACGTTT 
GAGGGCTGGC CTGCGTTGCT GTATAAAGCC ATCGACTCGA ATGGAGAGAA CATCGGCCCA 
ATCTACAACC ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTAGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTT GTCATCGTTA CATTTCAGGA ACAAGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CTTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACCGGGGT GTTCACCGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCAGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGA TTGGTGAAGC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACAT TGCTGTGGAC TTTTATTAAG TCCTTTCAGG CGCTCCCGTA TGTGGCCCTC 
CTCATAGCCA TGCTGTTCTT CATCTATGCG GTCATTGGCA TGCAGATGTT TGGGAAAGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAATAACT TCCAGACGTT TCCCCAGGCG 
GTGCTGCTGC TCTTCAGGTG TGCAACAGGT GAGGCCTGGC AGGAGATCAT GCTGGCCTGT 
CTCCCAGGGA AGCTCTGTGA CCCTGAGTCA GATTACAACC CCGGGGAGGA GTATACATGT 
GGGAGCAACT TTGCCATTGT CTATTTCATC AGTTTTTACA TGCTCTGTGC ATTTCTGATC 
ATCAATCTGT TTGTGGCTGT CATCATGGAT AATTTCGACT ATCTGACCCG GGACTGGTCT 
ATTTTGGGGC CTCACCATTT AGATGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAG 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTCTGC TTCGACGCAT CCAGCCTCCC 
CTGGGGTTTG GGAAGTTATG TCCACACAGG GTAGCGTGCA AGAGATTAGT TGCCATGAAC 
ATGCCTCTCA ACAGTGACGG GACAGTCATG TTTAATGCAA CCCTGTTTGC TTTGGTTCGA 
ACGGCTCTTA AGATCAAGAC CGAAGGGAAC CTGGAGCAAG CTAATGAAGA ACTTCGGGCT 
GTGATAAAGA AAATTTGGAA GAAAACCAGC ATGAAATTAC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAGTACCC TGCGAAGAAC 
ACCACAATTG CCCTACAGGC GGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CGTGTGATTT GCAAGATGAC GAGCCTGAGG AAACAAAACG AGAAGAAGAA 
GATGATGTGT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ATTCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAATTC CACCTGCAAG TGATACTGAG AAACCGCTGT TTCCTCCAGC AGGAAATTCG 
GTGTGTCATA ACCATCATAA CCATAATTCC ATAGGAAAGC AAGTTCCCAC CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTCCAAA GCTGCCCATG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC ATGTGTCTGA AAATGGGCAT CATTCTTCCC ACAAGCATGA CCGGGAGCCT 
CAGAGAAGGT CCAGTGTGAA AAGGTCCGAC TCAGGAGATG AACAGCTCCC AACTATTTGC 
CGGGAAGACC CAGAGATACA TGGCTATTTC AGGGACCCCC ACTGCTTGGG GGAGCAGGAG 
TATTTCAGTA GTGAGGAATG CTACGAGGAT GACAGCTCGC CCACCTGGAG CAGGCAAAAC 
TATGGCTACT ACAGCAGATA CCCAGGCAGA AACATCGACT CTGAGAGGCC CCGAGGCTAC 
CATCATCCCC AAGGATTCTT GGAGGACGAT GACTCGCCCG TTTGCTATGA TTCACGGAGA 
TCTCCAAGGA GACGCCTACT ACCTCCCACC CCAGCATCCC ACCGGAGATC CTCCTTCAAC 
TTTGAGTGCC TGCGCCGGCA GAGCAGCCAG GAAGAGGTCC CGTCGTCTCC CATCTTCCCC 
CATCGCACGG CCCTGCCTCT GCATCTAATG CAGCAACAGA TCATGGCAGT TGCCGGCCTA 
GATTCAAGTA AAGCCCAGAA GTACTCACCG AGTCACTCGA CCCGGTCGTG GGCCACCCCT 
CCAGCAACCC CTCCCTACCG GGACTGGACA CCGTGCTACA CCCCCCTGAT CCAAGTGGAG 
CAGTCAGAGG CCCTGGACCA GGTGAACGGC AGCCTGCCGT CCCTGCACCG CAGCTCCTGG 
TACACAGACG AGCCCGACAT CTCCTACCGG ACTTTCACAC CAGCCAGCCT GACTGTCCCC 
AGCAGCTTCC GGAACAAAAA CAGCGACAAG CAGAGGAGTG CGGACAGCTT GGTGGAGGCA 
GTCCTGATAT CCGAAGGCTT GGGACGCTAT GCAAGGGACC CAAAATTTGT GTCAGCAACA 
AAACACGAAA TCGCTGATGC CTGTGACCTC ACCATCGACG AGATGGAGAG TGCAGCCAGC 
ACCCTGCTTA ATGGGAACGT GCGTCCCCGA GCCAACGGGG ATGTGGGCCC CCTCTCACAC 
CGGCAGGACT ATGAGCTACA GGACTTTGGT CCTGGCTACA GCGACGAAGA GCCAGACCCT 
GGGAGGGATG AGGAGGACCT GGCGGATGAA ATGATATGCA TCACCACCTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001122311.1
CDS	119..6532
Translation	MMMMMMMKKMQHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGENRGCCGSLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENIGPIYNHRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEDDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGNLEHVSENGHHSSHKHDREPQRRSSVKRSDSGDEQLPTICREDPEIHGYFRDPHCLGEQEYFSSEECYEDDSSPTWSRQNYGYYSRYPGRNIDSERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPASHRRSSFNFECLRRQSSQEEVPSSPIFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVEQSEALDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGNVRPRANGDVGPLSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	NM_001128839.2
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128839.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTTCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACTGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTTTT CTGTTTATCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATAGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC AAGCGGCAAA TCTGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTTCGAG TGTTGCGACC ACTTCGACTA GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATCGTAGCT GAAGAGGACC CAGCTCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTAC TGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCG 
AACGGAGGCA TCACCAACTT TGATAACTTT GCCTTTGCCA TGCTTACTGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCAAA AGCACGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCTGAGGA CATCGATCCG GAGAATGAGG AAGAAGGAGG AGAGGAAGGC 
AAACGAAATA CTAGCATGCC CACCAGCGAG ACTGAGTCTG TGAACACAGA GAACGTCAGC 
GGTGAAGGCG AGAACCGAGG CTGCTGTGGA AGTCTCTGTC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGA TTCAATCGCA GAAGATGTAG GGCCGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATC GTCCTGGTGT TTCTGAACAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCAGATTGG TTGACACAGA TTCAAGATAT TGCCAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGCCTCCAA 
GCATATTTCG TCTCTCTTTT CAACCGGTTT GATTGCTTCG TGGTGTGTGG TGGAATCACT 
GAGACGATCT TGGTGGAACT GGAAATCATG TCTCCCCTGG GGATCTCTGT GTTTCGGTGT 
GTGCGCCTCT TAAGAATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCATCCTTAT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATTATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CACTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGACTG GAATGCTGTG ATGTACGATG GCATCATGGC TTACGGGGGC 
CCATCCTCTT CAGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA AGAAGCGGAA GAAAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTAG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA CTATAGAGAA GAGGATGAAG ACAAGGACCC CTATCCGCCT 
TGCGATGTGC CAGTAGGGGA AGAGGAAGAG GAAGAGGAGG AGGATGAACC TGAGGTTCCT 
GCCGGACCCC GTCCTCGAAG GATCTCGGAG TTGAACATGA AGGAAAAAAT TGCCCCCATC 
CCTGAAGGGA GCGCTTTCTT CATTCTTAGC AAGACCAACC CGATCCGCGT AGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC GATACTGGGT 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACTGTTGAGA TCCTGTTGAA GATGACAACT 
TTTGGAGCTT TCCTCCACAA AGGGGCCTTC TGCAGGAACT ACTTCAATTT GCTGGATATG 
CTGGTGGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGT CCCCTCAGGG CCATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG CGTCTTCGTG GCCATCCGGA CCATCGGCAA CATCATGATC 
GTCACCACCC TCCTGCAGTT CATGTTTGCC TGTATCGGGG TCCAGTTGTT CAAGGGGAAG 
TTCTATCGCT GTACGGATGA AGCCAAAAGT AACCCTGAAG AATGCAGGGG ACTTTTCATC 
CTCTACAAGG ATGGGGATGT TGACAGTCCT GTGGTCCGTG AACGGATCTG GCAAAACAGT 
GATTTCAACT TCGACAACGT CCTCTCTGCT ATGATGGCGC TCTTCACAGT CTCCACGTTT 
GAGGGCTGGC CTGCGTTGCT GTATAAAGCC ATCGACTCGA ATGGAGAGAA CATCGGCCCA 
ATCTACAACC ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTAGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTT GTCATCGTTA CATTTCAGGA ACAAGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CTTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACCGGGGT GTTCACCGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCAGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGA TTGGTGAAGC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACAT TGCTGTGGAC TTTTATTAAG TCCTTTCAGG CGCTCCCGTA TGTGGCCCTC 
CTCATAGCCA TGCTGTTCTT CATCTATGCG GTCATTGGCA TGCAGATGTT TGGGAAAGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAATAACT TCCAGACGTT TCCCCAGGCG 
GTGCTGCTGC TCTTCAGGTG TGCAACAGGT GAGGCCTGGC AGGAGATCAT GCTGGCCTGT 
CTCCCAGGGA AGCTCTGTGA CCCTGAGTCA GATTACAACC CCGGGGAGGA GTATACATGT 
GGGAGCAACT TTGCCATTGT CTATTTCATC AGTTTTTACA TGCTCTGTGC ATTTCTGATC 
ATCAATCTGT TTGTGGCTGT CATCATGGAT AATTTCGACT ATCTGACCCG GGACTGGTCT 
ATTTTGGGGC CTCACCATTT AGATGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAG 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTCTGC TTCGACGCAT CCAGCCTCCC 
CTGGGGTTTG GGAAGTTATG TCCACACAGG GTAGCGTGCA AGAGATTAGT TGCCATGAAC 
ATGCCTCTCA ACAGTGACGG GACAGTCATG TTTAATGCAA CCCTGTTTGC TTTGGTTCGA 
ACGGCTCTTA AGATCAAGAC CGAAGGGAAC CTGGAGCAAG CTAATGAAGA ACTTCGGGCT 
GTGATAAAGA AAATTTGGAA GAAAACCAGC ATGAAATTAC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAGTACCC TGCGAAGAAC 
ACCACAATTG CCCTACAGGC GGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CGTGTGATTT GCAAGATGAC GAGCCTGAGG AAACAAAACG AGAAGAAGAA 
GATGATGTGT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ATTCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAATTC CACCTGCAAG TGATACTGAG AAACCGCTGT TTCCTCCAGC AGGAAATTCG 
GTGTGTCATA ACCATCATAA CCATAATTCC ATAGGAAAGC AAGTTCCCAC CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTCCAAA GCTGCCCATG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC ATGTGTCTGA AAATGGGCAT CATTCTTCCC ACAAGCATGA CCGGGAGCCT 
CAGAGAAGGT CCAGTGTGAA AAGGTCCGAC TCAGGAGATG AACAGCTCCC AACTATTTGC 
CGGGAAGACC CAGAGATACA TGGCTATTTC AGGGACCCCC ACTGCTTGGG GGAGCAGGAG 
TATTTCAGTA GTGAGGAATG CTACGAGGAT GACAGCTCGC CCACCTGGAG CAGGCAAAAC 
TATGGCTACT ACAGCAGATA CCCAGGCAGA AACATCGACT CTGAGAGGCC CCGAGGCTAC 
CATCATCCCC AAGGATTCTT GGAGGACGAT GACTCGCCCG TTTGCTATGA TTCACGGAGA 
TCTCCAAGGA GACGCCTACT ACCTCCCACC CCAGCATCCC ACCGGAGATC CTCCTTCAAC 
TTTGAGTGCC TGCGCCGGCA GAGCAGCCAG GAAGAGGTCC CGTCGTCTCC CATCTTCCCC 
CATCGCACGG CCCTGCCTCT GCATCTAATG CAGCAACAGA TCATGGCAGT TGCCGGCCTA 
GATTCAAGTA AAGCCCAGAA GTACTCACCG AGTCACTCGA CCCGGTCGTG GGCCACCCCT 
CCAGCAACCC CTCCCTACCG GGACTGGACA CCGTGCTACA CCCCCCTGAT CCAAGTGGAG 
CAGTCAGAGG CCCTGGACCA GGTGAACGGC AGCCTGCCGT CCCTGCACCG CAGCTCCTGG 
TACACAGACG AGCCCGACAT CTCCTACCGG ACTTTCACAC CAGCCAGCCT GACTGTCCCC 
AGCAGCTTCC GGAACAAAAA CAGCGACAAG CAGAGGAGTG CGGACAGCTT GGTGGAGGCA 
GTCCTGATAT CCGAAGGCTT GGGACGCTAT GCAAGGGACC CAAAATTTGT GTCAGCAACA 
AAACACGAAA TCGCTGATGC CTGTGACCTC ACCATCGACG AGATGGAGAG TGCAGCCAGC 
ACCCTGCTTA ATGGGAACGT GCGTCCCCGA GCCAACGGGG ATGTGGGCCC CCTCTCACAC 
CGGCAGGACT ATGAGCTACA GGACTTTGGT CCTGGCTACA GCGACGAAGA GCCAGACCCT 
GGGAGGGATG AGGAGGACCT GGCGGATGAA ATGATATGCA TCACCACCTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( NM_001128839.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001122311.1 Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant 3, mRNA.

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CloneID	OHu10032
Clone ID Related Accession (Same CDS sequence)	NM_001128839.2 , NM_001128839.3
Accession Version	NM_001128839.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005905.1, M76558.1, M83566.1, EU363339.1, AC132810.2, AW008794.1 and AC012467.9. On May 31, 2019 this sequence version replaced NM_001128839.2. Transcript Variant: This variant (3) includes alternate in-frame exons, compared to variant 1, resulting in a shorter protein (isoform c), compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU363339.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144120, SAMEA2145240 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTTCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACTGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTTTT CTGTTTATCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATAGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC AAGCGGCAAA TCTGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTTCGAG TGTTGCGACC ACTTCGACTA GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATCGTAGCT GAAGAGGACC CAGCTCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTAC TGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCG 
AACGGAGGCA TCACCAACTT TGATAACTTT GCCTTTGCCA TGCTTACTGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCAAA AGCACGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCTGAGGA CATCGATCCG GAGAATGAGG AAGAAGGAGG AGAGGAAGGC 
AAACGAAATA CTAGCATGCC CACCAGCGAG ACTGAGTCTG TGAACACAGA GAACGTCAGC 
GGTGAAGGCG AGAACCGAGG CTGCTGTGGA AGTCTCTGTC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGA TTCAATCGCA GAAGATGTAG GGCCGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATC GTCCTGGTGT TTCTGAACAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCAGATTGG TTGACACAGA TTCAAGATAT TGCCAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGCCTCCAA 
GCATATTTCG TCTCTCTTTT CAACCGGTTT GATTGCTTCG TGGTGTGTGG TGGAATCACT 
GAGACGATCT TGGTGGAACT GGAAATCATG TCTCCCCTGG GGATCTCTGT GTTTCGGTGT 
GTGCGCCTCT TAAGAATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCATCCTTAT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATTATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CACTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGACTG GAATGCTGTG ATGTACGATG GCATCATGGC TTACGGGGGC 
CCATCCTCTT CAGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA AGAAGCGGAA GAAAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTAG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA CTATAGAGAA GAGGATGAAG ACAAGGACCC CTATCCGCCT 
TGCGATGTGC CAGTAGGGGA AGAGGAAGAG GAAGAGGAGG AGGATGAACC TGAGGTTCCT 
GCCGGACCCC GTCCTCGAAG GATCTCGGAG TTGAACATGA AGGAAAAAAT TGCCCCCATC 
CCTGAAGGGA GCGCTTTCTT CATTCTTAGC AAGACCAACC CGATCCGCGT AGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC GATACTGGGT 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACTGTTGAGA TCCTGTTGAA GATGACAACT 
TTTGGAGCTT TCCTCCACAA AGGGGCCTTC TGCAGGAACT ACTTCAATTT GCTGGATATG 
CTGGTGGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGT CCCCTCAGGG CCATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG CGTCTTCGTG GCCATCCGGA CCATCGGCAA CATCATGATC 
GTCACCACCC TCCTGCAGTT CATGTTTGCC TGTATCGGGG TCCAGTTGTT CAAGGGGAAG 
TTCTATCGCT GTACGGATGA AGCCAAAAGT AACCCTGAAG AATGCAGGGG ACTTTTCATC 
CTCTACAAGG ATGGGGATGT TGACAGTCCT GTGGTCCGTG AACGGATCTG GCAAAACAGT 
GATTTCAACT TCGACAACGT CCTCTCTGCT ATGATGGCGC TCTTCACAGT CTCCACGTTT 
GAGGGCTGGC CTGCGTTGCT GTATAAAGCC ATCGACTCGA ATGGAGAGAA CATCGGCCCA 
ATCTACAACC ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTAGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTT GTCATCGTTA CATTTCAGGA ACAAGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CTTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACCGGGGT GTTCACCGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCAGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGA TTGGTGAAGC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACAT TGCTGTGGAC TTTTATTAAG TCCTTTCAGG CGCTCCCGTA TGTGGCCCTC 
CTCATAGCCA TGCTGTTCTT CATCTATGCG GTCATTGGCA TGCAGATGTT TGGGAAAGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAATAACT TCCAGACGTT TCCCCAGGCG 
GTGCTGCTGC TCTTCAGGTG TGCAACAGGT GAGGCCTGGC AGGAGATCAT GCTGGCCTGT 
CTCCCAGGGA AGCTCTGTGA CCCTGAGTCA GATTACAACC CCGGGGAGGA GTATACATGT 
GGGAGCAACT TTGCCATTGT CTATTTCATC AGTTTTTACA TGCTCTGTGC ATTTCTGATC 
ATCAATCTGT TTGTGGCTGT CATCATGGAT AATTTCGACT ATCTGACCCG GGACTGGTCT 
ATTTTGGGGC CTCACCATTT AGATGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAG 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTCTGC TTCGACGCAT CCAGCCTCCC 
CTGGGGTTTG GGAAGTTATG TCCACACAGG GTAGCGTGCA AGAGATTAGT TGCCATGAAC 
ATGCCTCTCA ACAGTGACGG GACAGTCATG TTTAATGCAA CCCTGTTTGC TTTGGTTCGA 
ACGGCTCTTA AGATCAAGAC CGAAGGGAAC CTGGAGCAAG CTAATGAAGA ACTTCGGGCT 
GTGATAAAGA AAATTTGGAA GAAAACCAGC ATGAAATTAC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAGTACCC TGCGAAGAAC 
ACCACAATTG CCCTACAGGC GGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CGTGTGATTT GCAAGATGAC GAGCCTGAGG AAACAAAACG AGAAGAAGAA 
GATGATGTGT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ATTCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAATTC CACCTGCAAG TGATACTGAG AAACCGCTGT TTCCTCCAGC AGGAAATTCG 
GTGTGTCATA ACCATCATAA CCATAATTCC ATAGGAAAGC AAGTTCCCAC CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTCCAAA GCTGCCCATG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC ATGTGTCTGA AAATGGGCAT CATTCTTCCC ACAAGCATGA CCGGGAGCCT 
CAGAGAAGGT CCAGTGTGAA AAGGTCCGAC TCAGGAGATG AACAGCTCCC AACTATTTGC 
CGGGAAGACC CAGAGATACA TGGCTATTTC AGGGACCCCC ACTGCTTGGG GGAGCAGGAG 
TATTTCAGTA GTGAGGAATG CTACGAGGAT GACAGCTCGC CCACCTGGAG CAGGCAAAAC 
TATGGCTACT ACAGCAGATA CCCAGGCAGA AACATCGACT CTGAGAGGCC CCGAGGCTAC 
CATCATCCCC AAGGATTCTT GGAGGACGAT GACTCGCCCG TTTGCTATGA TTCACGGAGA 
TCTCCAAGGA GACGCCTACT ACCTCCCACC CCAGCATCCC ACCGGAGATC CTCCTTCAAC 
TTTGAGTGCC TGCGCCGGCA GAGCAGCCAG GAAGAGGTCC CGTCGTCTCC CATCTTCCCC 
CATCGCACGG CCCTGCCTCT GCATCTAATG CAGCAACAGA TCATGGCAGT TGCCGGCCTA 
GATTCAAGTA AAGCCCAGAA GTACTCACCG AGTCACTCGA CCCGGTCGTG GGCCACCCCT 
CCAGCAACCC CTCCCTACCG GGACTGGACA CCGTGCTACA CCCCCCTGAT CCAAGTGGAG 
CAGTCAGAGG CCCTGGACCA GGTGAACGGC AGCCTGCCGT CCCTGCACCG CAGCTCCTGG 
TACACAGACG AGCCCGACAT CTCCTACCGG ACTTTCACAC CAGCCAGCCT GACTGTCCCC 
AGCAGCTTCC GGAACAAAAA CAGCGACAAG CAGAGGAGTG CGGACAGCTT GGTGGAGGCA 
GTCCTGATAT CCGAAGGCTT GGGACGCTAT GCAAGGGACC CAAAATTTGT GTCAGCAACA 
AAACACGAAA TCGCTGATGC CTGTGACCTC ACCATCGACG AGATGGAGAG TGCAGCCAGC 
ACCCTGCTTA ATGGGAACGT GCGTCCCCGA GCCAACGGGG ATGTGGGCCC CCTCTCACAC 
CGGCAGGACT ATGAGCTACA GGACTTTGGT CCTGGCTACA GCGACGAAGA GCCAGACCCT 
GGGAGGGATG AGGAGGACCT GGCGGATGAA ATGATATGCA TCACCACCTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001122311.1
CDS	557..6970
Translation	MMMMMMMKKMQHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGENRGCCGSLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENIGPIYNHRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEDDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGNLEHVSENGHHSSHKHDREPQRRSSVKRSDSGDEQLPTICREDPEIHGYFRDPHCLGEQEYFSSEECYEDDSSPTWSRQNYGYYSRYPGRNIDSERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPASHRRSSFNFECLRRQSSQEEVPSSPIFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVEQSEALDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGNVRPRANGDVGPLSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	NM_001128839.3
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128839.3

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTTCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACTGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTTTT CTGTTTATCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATAGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC AAGCGGCAAA TCTGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTTCGAG TGTTGCGACC ACTTCGACTA GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATCGTAGCT GAAGAGGACC CAGCTCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTAC TGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCG 
AACGGAGGCA TCACCAACTT TGATAACTTT GCCTTTGCCA TGCTTACTGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCAAA AGCACGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCTGAGGA CATCGATCCG GAGAATGAGG AAGAAGGAGG AGAGGAAGGC 
AAACGAAATA CTAGCATGCC CACCAGCGAG ACTGAGTCTG TGAACACAGA GAACGTCAGC 
GGTGAAGGCG AGAACCGAGG CTGCTGTGGA AGTCTCTGTC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGA TTCAATCGCA GAAGATGTAG GGCCGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATC GTCCTGGTGT TTCTGAACAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCAGATTGG TTGACACAGA TTCAAGATAT TGCCAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGCCTCCAA 
GCATATTTCG TCTCTCTTTT CAACCGGTTT GATTGCTTCG TGGTGTGTGG TGGAATCACT 
GAGACGATCT TGGTGGAACT GGAAATCATG TCTCCCCTGG GGATCTCTGT GTTTCGGTGT 
GTGCGCCTCT TAAGAATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCATCCTTAT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATTATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CACTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGACTG GAATGCTGTG ATGTACGATG GCATCATGGC TTACGGGGGC 
CCATCCTCTT CAGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA AGAAGCGGAA GAAAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTAG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA CTATAGAGAA GAGGATGAAG ACAAGGACCC CTATCCGCCT 
TGCGATGTGC CAGTAGGGGA AGAGGAAGAG GAAGAGGAGG AGGATGAACC TGAGGTTCCT 
GCCGGACCCC GTCCTCGAAG GATCTCGGAG TTGAACATGA AGGAAAAAAT TGCCCCCATC 
CCTGAAGGGA GCGCTTTCTT CATTCTTAGC AAGACCAACC CGATCCGCGT AGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC GATACTGGGT 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACTGTTGAGA TCCTGTTGAA GATGACAACT 
TTTGGAGCTT TCCTCCACAA AGGGGCCTTC TGCAGGAACT ACTTCAATTT GCTGGATATG 
CTGGTGGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGT CCCCTCAGGG CCATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG CGTCTTCGTG GCCATCCGGA CCATCGGCAA CATCATGATC 
GTCACCACCC TCCTGCAGTT CATGTTTGCC TGTATCGGGG TCCAGTTGTT CAAGGGGAAG 
TTCTATCGCT GTACGGATGA AGCCAAAAGT AACCCTGAAG AATGCAGGGG ACTTTTCATC 
CTCTACAAGG ATGGGGATGT TGACAGTCCT GTGGTCCGTG AACGGATCTG GCAAAACAGT 
GATTTCAACT TCGACAACGT CCTCTCTGCT ATGATGGCGC TCTTCACAGT CTCCACGTTT 
GAGGGCTGGC CTGCGTTGCT GTATAAAGCC ATCGACTCGA ATGGAGAGAA CATCGGCCCA 
ATCTACAACC ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTAGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTT GTCATCGTTA CATTTCAGGA ACAAGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CTTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACCGGGGT GTTCACCGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCAGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGA TTGGTGAAGC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACAT TGCTGTGGAC TTTTATTAAG TCCTTTCAGG CGCTCCCGTA TGTGGCCCTC 
CTCATAGCCA TGCTGTTCTT CATCTATGCG GTCATTGGCA TGCAGATGTT TGGGAAAGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAATAACT TCCAGACGTT TCCCCAGGCG 
GTGCTGCTGC TCTTCAGGTG TGCAACAGGT GAGGCCTGGC AGGAGATCAT GCTGGCCTGT 
CTCCCAGGGA AGCTCTGTGA CCCTGAGTCA GATTACAACC CCGGGGAGGA GTATACATGT 
GGGAGCAACT TTGCCATTGT CTATTTCATC AGTTTTTACA TGCTCTGTGC ATTTCTGATC 
ATCAATCTGT TTGTGGCTGT CATCATGGAT AATTTCGACT ATCTGACCCG GGACTGGTCT 
ATTTTGGGGC CTCACCATTT AGATGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAG 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTCTGC TTCGACGCAT CCAGCCTCCC 
CTGGGGTTTG GGAAGTTATG TCCACACAGG GTAGCGTGCA AGAGATTAGT TGCCATGAAC 
ATGCCTCTCA ACAGTGACGG GACAGTCATG TTTAATGCAA CCCTGTTTGC TTTGGTTCGA 
ACGGCTCTTA AGATCAAGAC CGAAGGGAAC CTGGAGCAAG CTAATGAAGA ACTTCGGGCT 
GTGATAAAGA AAATTTGGAA GAAAACCAGC ATGAAATTAC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAGTACCC TGCGAAGAAC 
ACCACAATTG CCCTACAGGC GGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CGTGTGATTT GCAAGATGAC GAGCCTGAGG AAACAAAACG AGAAGAAGAA 
GATGATGTGT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ATTCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAATTC CACCTGCAAG TGATACTGAG AAACCGCTGT TTCCTCCAGC AGGAAATTCG 
GTGTGTCATA ACCATCATAA CCATAATTCC ATAGGAAAGC AAGTTCCCAC CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTCCAAA GCTGCCCATG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC ATGTGTCTGA AAATGGGCAT CATTCTTCCC ACAAGCATGA CCGGGAGCCT 
CAGAGAAGGT CCAGTGTGAA AAGGTCCGAC TCAGGAGATG AACAGCTCCC AACTATTTGC 
CGGGAAGACC CAGAGATACA TGGCTATTTC AGGGACCCCC ACTGCTTGGG GGAGCAGGAG 
TATTTCAGTA GTGAGGAATG CTACGAGGAT GACAGCTCGC CCACCTGGAG CAGGCAAAAC 
TATGGCTACT ACAGCAGATA CCCAGGCAGA AACATCGACT CTGAGAGGCC CCGAGGCTAC 
CATCATCCCC AAGGATTCTT GGAGGACGAT GACTCGCCCG TTTGCTATGA TTCACGGAGA 
TCTCCAAGGA GACGCCTACT ACCTCCCACC CCAGCATCCC ACCGGAGATC CTCCTTCAAC 
TTTGAGTGCC TGCGCCGGCA GAGCAGCCAG GAAGAGGTCC CGTCGTCTCC CATCTTCCCC 
CATCGCACGG CCCTGCCTCT GCATCTAATG CAGCAACAGA TCATGGCAGT TGCCGGCCTA 
GATTCAAGTA AAGCCCAGAA GTACTCACCG AGTCACTCGA CCCGGTCGTG GGCCACCCCT 
CCAGCAACCC CTCCCTACCG GGACTGGACA CCGTGCTACA CCCCCCTGAT CCAAGTGGAG 
CAGTCAGAGG CCCTGGACCA GGTGAACGGC AGCCTGCCGT CCCTGCACCG CAGCTCCTGG 
TACACAGACG AGCCCGACAT CTCCTACCGG ACTTTCACAC CAGCCAGCCT GACTGTCCCC 
AGCAGCTTCC GGAACAAAAA CAGCGACAAG CAGAGGAGTG CGGACAGCTT GGTGGAGGCA 
GTCCTGATAT CCGAAGGCTT GGGACGCTAT GCAAGGGACC CAAAATTTGT GTCAGCAACA 
AAACACGAAA TCGCTGATGC CTGTGACCTC ACCATCGACG AGATGGAGAG TGCAGCCAGC 
ACCCTGCTTA ATGGGAACGT GCGTCCCCGA GCCAACGGGG ATGTGGGCCC CCTCTCACAC 
CGGCAGGACT ATGAGCTACA GGACTTTGGT CCTGGCTACA GCGACGAAGA GCCAGACCCT 
GGGAGGGATG AGGAGGACCT GGCGGATGAA ATGATATGCA TCACCACCTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( NM_001128840.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001122312.1 Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant 2, mRNA.

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CloneID	OHu10146
Clone ID Related Accession (Same CDS sequence)	NM_001128840.2 , NM_001128840.3
Accession Version	NM_001128840.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M76558.1, M83566.1, AC132810.2, AB209171.1 and AW008794.1. On Dec 4, 2012 this sequence version replaced NM_001128840.1. Transcript Variant: This variant (2) includes alternate in-frame exons, compared to variant 1, resulting in a shorter protein (isoform b), compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M76558.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145544, SAMEA2146411 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTTCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACTGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTTTT CTGTTTATCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATAGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC AAGCGGCAAA TCTGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTTCGAG TGTTGCGACC ACTTCGACTA GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATCGTAGCT GAAGAGGACC CAGCTCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTAC TGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCG 
AACGGAGGCA TCACCAACTT TGATAACTTT GCCTTTGCCA TGCTTACTGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCAAA AGCACGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCTGAGGA CATCGATCCG GAGAATGAGG AAGAAGGAGG AGAGGAAGGC 
AAACGAAATA CTAGCATGCC CACCAGCGAG ACTGAGTCTG TGAACACAGA GAACGTCAGC 
GGTGAAGGCG AGAACCGAGG CTGCTGTGGA AGTCTCTGTC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGA TTCAATCGCA GAAGATGTAG GGCCGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATC GTCCTGGTGT TTCTGAACAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCAGATTGG TTGACACAGA TTCAAGATAT TGCCAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGCCTCCAA 
GCATATTTCG TCTCTCTTTT CAACCGGTTT GATTGCTTCG TGGTGTGTGG TGGAATCACT 
GAGACGATCT TGGTGGAACT GGAAATCATG TCTCCCCTGG GGATCTCTGT GTTTCGGTGT 
GTGCGCCTCT TAAGAATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCATCCTTAT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATTATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CACTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGACTG GAATGCTGTG ATGTACGATG GCATCATGGC TTACGGGGGC 
CCATCCTCTT CAGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA AGAAGCGGAA GAAAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTAG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA CTATAGAGAA GAGGATGAAG ACAAGGACCC CTATCCGCCT 
TGCGATGTGC CAGTAGGGGA AGAGGAAGAG GAAGAGGAGG AGGATGAACC TGAGGTTCCT 
GCCGGACCCC GTCCTCGAAG GATCTCGGAG TTGAACATGA AGGAAAAAAT TGCCCCCATC 
CCTGAAGGGA GCGCTTTCTT CATTCTTAGC AAGACCAACC CGATCCGCGT AGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC GATACTGGGT 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACTGTTGAGA TCCTGTTGAA GATGACAACT 
TTTGGAGCTT TCCTCCACAA AGGGGCCTTC TGCAGGAACT ACTTCAATTT GCTGGATATG 
CTGGTGGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGT CCCCTCAGGG CCATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG CGTCTTCGTG GCCATCCGGA CCATCGGCAA CATCATGATC 
GTCACCACCC TCCTGCAGTT CATGTTTGCC TGTATCGGGG TCCAGTTGTT CAAGGGGAAG 
TTCTATCGCT GTACGGATGA AGCCAAAAGT AACCCTGAAG AATGCAGGGG ACTTTTCATC 
CTCTACAAGG ATGGGGATGT TGACAGTCCT GTGGTCCGTG AACGGATCTG GCAAAACAGT 
GATTTCAACT TCGACAACGT CCTCTCTGCT ATGATGGCGC TCTTCACAGT CTCCACGTTT 
GAGGGCTGGC CTGCGTTGCT GTATAAAGCC ATCGACTCGA ATGGAGAGAA CATCGGCCCA 
ATCTACAACC ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTAGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTT GTCATCGTTA CATTTCAGGA ACAAGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CTTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACCGGGGT GTTCACCGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCAGAC CCAACTGAAA GTGAAAATGT CCCTGTCCCA 
ACTGCTACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGATTGGT GAAGCTTCTC AGCAGGGGGG AAGGCATCCG GACATTGCTG 
TGGACTTTTA TTAAGTCCTT TCAGGCGCTC CCGTATGTGG CCCTCCTCAT AGCCATGCTG 
TTCTTCATCT ATGCGGTCAT TGGCATGCAG ATGTTTGGGA AAGTTGCCAT GAGAGATAAC 
AACCAGATCA ATAGGAACAA TAACTTCCAG ACGTTTCCCC AGGCGGTGCT GCTGCTCTTC 
AGGTGTGCAA CAGGTGAGGC CTGGCAGGAG ATCATGCTGG CCTGTCTCCC AGGGAAGCTC 
TGTGACCCTG AGTCAGATTA CAACCCCGGG GAGGAGTATA CATGTGGGAG CAACTTTGCC 
ATTGTCTATT TCATCAGTTT TTACATGCTC TGTGCATTTC TGATCATCAA TCTGTTTGTG 
GCTGTCATCA TGGATAATTT CGACTATCTG ACCCGGGACT GGTCTATTTT GGGGCCTCAC 
CATTTAGATG AATTCAAAAG AATATGGTCA GAATATGACC CTGAGGCAAA GGGAAGGATA 
AAACACCTTG ATGTGGTCAC TCTGCTTCGA CGCATCCAGC CTCCCCTGGG GTTTGGGAAG 
TTATGTCCAC ACAGGGTAGC GTGCAAGAGA TTAGTTGCCA TGAACATGCC TCTCAACAGT 
GACGGGACAG TCATGTTTAA TGCAACCCTG TTTGCTTTGG TTCGAACGGC TCTTAAGATC 
AAGACCGAAG GGAACCTGGA GCAAGCTAAT GAAGAACTTC GGGCTGTGAT AAAGAAAATT 
TGGAAGAAAA CCAGCATGAA ATTACTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AACAAGGACT GGTGGGAAAG TACCCTGCGA AGAACACCAC AATTGCCCTA 
CAGGCGGGAT TAAGGACACT GCATGACATT GGGCCAGAAA TCCGGCGTGC TATATCGTGT 
GATTTGCAAG ATGACGAGCC TGAGGAAACA AAACGAGAAG AAGAAGATGA TGTGTTCAAA 
AGAAATGGTG CCCTGCTTGG AAACCATGTC AATCATGTTA ATAGTGATAG GAGAGATTCC 
CTTCAGCAGA CCAATACCAC CCACCGTCCC CTGCATGTCC AAAGGCCTTC AATTCCACCT 
GCAAGTGATA CTGAGAAACC GCTGTTTCCT CCAGCAGGAA ATTCGGTGTG TCATAACCAT 
CATAACCATA ATTCCATAGG AAAGCAAGTT CCCACCTCAA CAAATGCCAA TCTCAATAAT 
GCCAATATGT CCAAAGCTGC CCATGGAAAG CGGCCCAGCA TTGGGAACCT TGAGCATGTG 
TCTGAAAATG GGCATCATTC TTCCCACAAG CATGACCGGG AGCCTCAGAG AAGGTCCAGT 
GTGAAAAGAA CCCGCTATTA TGAAACTTAC ATTAGGTCCG ACTCAGGAGA TGAACAGCTC 
CCAACTATTT GCCGGGAAGA CCCAGAGATA CATGGCTATT TCAGGGACCC CCACTGCTTG 
GGGGAGCAGG AGTATTTCAG TAGTGAGGAA TGCTACGAGG ATGACAGCTC GCCCACCTGG 
AGCAGGCAAA ACTATGGCTA CTACAGCAGA TACCCAGGCA GAAACATCGA CTCTGAGAGG 
CCCCGAGGCT ACCATCATCC CCAAGGATTC TTGGAGGACG ATGACTCGCC CGTTTGCTAT 
GATTCACGGA GATCTCCAAG GAGACGCCTA CTACCTCCCA CCCCAGCATC CCACCGGAGA 
TCCTCCTTCA ACTTTGAGTG CCTGCGCCGG CAGAGCAGCC AGGAAGAGGT CCCGTCGTCT 
CCCATCTTCC CCCATCGCAC GGCCCTGCCT CTGCATCTAA TGCAGCAACA GATCATGGCA 
GTTGCCGGCC TAGATTCAAG TAAAGCCCAG AAGTACTCAC CGAGTCACTC GACCCGGTCG 
TGGGCCACCC CTCCAGCAAC CCCTCCCTAC CGGGACTGGA CACCGTGCTA CACCCCCCTG 
ATCCAAGTGG AGCAGTCAGA GGCCCTGGAC CAGGTGAACG GCAGCCTGCC GTCCCTGCAC 
CGCAGCTCCT GGTACACAGA CGAGCCCGAC ATCTCCTACC GGACTTTCAC ACCAGCCAGC 
CTGACTGTCC CCAGCAGCTT CCGGAACAAA AACAGCGACA AGCAGAGGAG TGCGGACAGC 
TTGGTGGAGG CAGTCCTGAT ATCCGAAGGC TTGGGACGCT ATGCAAGGGA CCCAAAATTT 
GTGTCAGCAA CAAAACACGA AATCGCTGAT GCCTGTGACC TCACCATCGA CGAGATGGAG 
AGTGCAGCCA GCACCCTGCT TAATGGGAAC GTGCGTCCCC GAGCCAACGG GGATGTGGGC 
CCCCTCTCAC ACCGGCAGGA CTATGAGCTA CAGGACTTTG GTCCTGGCTA CAGCGACGAA 
GAGCCAGACC CTGGGAGGGA TGAGGAGGAC CTGGCGGATG AAATGATATG CATCACCACC 
TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001122312.1
CDS	119..6604
Translation	MMMMMMMKKMQHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGENRGCCGSLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENIGPIYNHRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADPTESENVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEDDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGNLEHVSENGHHSSHKHDREPQRRSSVKRTRYYETYIRSDSGDEQLPTICREDPEIHGYFRDPHCLGEQEYFSSEECYEDDSSPTWSRQNYGYYSRYPGRNIDSERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPASHRRSSFNFECLRRQSSQEEVPSSPIFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVEQSEALDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGNVRPRANGDVGPLSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	NM_001128840.2
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128840.2

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTTCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACTGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTTTT CTGTTTATCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATAGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC AAGCGGCAAA TCTGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTTCGAG TGTTGCGACC ACTTCGACTA GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATCGTAGCT GAAGAGGACC CAGCTCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTAC TGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCG 
AACGGAGGCA TCACCAACTT TGATAACTTT GCCTTTGCCA TGCTTACTGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCAAA AGCACGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCTGAGGA CATCGATCCG GAGAATGAGG AAGAAGGAGG AGAGGAAGGC 
AAACGAAATA CTAGCATGCC CACCAGCGAG ACTGAGTCTG TGAACACAGA GAACGTCAGC 
GGTGAAGGCG AGAACCGAGG CTGCTGTGGA AGTCTCTGTC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGA TTCAATCGCA GAAGATGTAG GGCCGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATC GTCCTGGTGT TTCTGAACAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCAGATTGG TTGACACAGA TTCAAGATAT TGCCAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGCCTCCAA 
GCATATTTCG TCTCTCTTTT CAACCGGTTT GATTGCTTCG TGGTGTGTGG TGGAATCACT 
GAGACGATCT TGGTGGAACT GGAAATCATG TCTCCCCTGG GGATCTCTGT GTTTCGGTGT 
GTGCGCCTCT TAAGAATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCATCCTTAT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATTATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CACTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGACTG GAATGCTGTG ATGTACGATG GCATCATGGC TTACGGGGGC 
CCATCCTCTT CAGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA AGAAGCGGAA GAAAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTAG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA CTATAGAGAA GAGGATGAAG ACAAGGACCC CTATCCGCCT 
TGCGATGTGC CAGTAGGGGA AGAGGAAGAG GAAGAGGAGG AGGATGAACC TGAGGTTCCT 
GCCGGACCCC GTCCTCGAAG GATCTCGGAG TTGAACATGA AGGAAAAAAT TGCCCCCATC 
CCTGAAGGGA GCGCTTTCTT CATTCTTAGC AAGACCAACC CGATCCGCGT AGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC GATACTGGGT 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACTGTTGAGA TCCTGTTGAA GATGACAACT 
TTTGGAGCTT TCCTCCACAA AGGGGCCTTC TGCAGGAACT ACTTCAATTT GCTGGATATG 
CTGGTGGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGT CCCCTCAGGG CCATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG CGTCTTCGTG GCCATCCGGA CCATCGGCAA CATCATGATC 
GTCACCACCC TCCTGCAGTT CATGTTTGCC TGTATCGGGG TCCAGTTGTT CAAGGGGAAG 
TTCTATCGCT GTACGGATGA AGCCAAAAGT AACCCTGAAG AATGCAGGGG ACTTTTCATC 
CTCTACAAGG ATGGGGATGT TGACAGTCCT GTGGTCCGTG AACGGATCTG GCAAAACAGT 
GATTTCAACT TCGACAACGT CCTCTCTGCT ATGATGGCGC TCTTCACAGT CTCCACGTTT 
GAGGGCTGGC CTGCGTTGCT GTATAAAGCC ATCGACTCGA ATGGAGAGAA CATCGGCCCA 
ATCTACAACC ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTAGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTT GTCATCGTTA CATTTCAGGA ACAAGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CTTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACCGGGGT GTTCACCGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCAGAC CCAACTGAAA GTGAAAATGT CCCTGTCCCA 
ACTGCTACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGATTGGT GAAGCTTCTC AGCAGGGGGG AAGGCATCCG GACATTGCTG 
TGGACTTTTA TTAAGTCCTT TCAGGCGCTC CCGTATGTGG CCCTCCTCAT AGCCATGCTG 
TTCTTCATCT ATGCGGTCAT TGGCATGCAG ATGTTTGGGA AAGTTGCCAT GAGAGATAAC 
AACCAGATCA ATAGGAACAA TAACTTCCAG ACGTTTCCCC AGGCGGTGCT GCTGCTCTTC 
AGGTGTGCAA CAGGTGAGGC CTGGCAGGAG ATCATGCTGG CCTGTCTCCC AGGGAAGCTC 
TGTGACCCTG AGTCAGATTA CAACCCCGGG GAGGAGTATA CATGTGGGAG CAACTTTGCC 
ATTGTCTATT TCATCAGTTT TTACATGCTC TGTGCATTTC TGATCATCAA TCTGTTTGTG 
GCTGTCATCA TGGATAATTT CGACTATCTG ACCCGGGACT GGTCTATTTT GGGGCCTCAC 
CATTTAGATG AATTCAAAAG AATATGGTCA GAATATGACC CTGAGGCAAA GGGAAGGATA 
AAACACCTTG ATGTGGTCAC TCTGCTTCGA CGCATCCAGC CTCCCCTGGG GTTTGGGAAG 
TTATGTCCAC ACAGGGTAGC GTGCAAGAGA TTAGTTGCCA TGAACATGCC TCTCAACAGT 
GACGGGACAG TCATGTTTAA TGCAACCCTG TTTGCTTTGG TTCGAACGGC TCTTAAGATC 
AAGACCGAAG GGAACCTGGA GCAAGCTAAT GAAGAACTTC GGGCTGTGAT AAAGAAAATT 
TGGAAGAAAA CCAGCATGAA ATTACTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AACAAGGACT GGTGGGAAAG TACCCTGCGA AGAACACCAC AATTGCCCTA 
CAGGCGGGAT TAAGGACACT GCATGACATT GGGCCAGAAA TCCGGCGTGC TATATCGTGT 
GATTTGCAAG ATGACGAGCC TGAGGAAACA AAACGAGAAG AAGAAGATGA TGTGTTCAAA 
AGAAATGGTG CCCTGCTTGG AAACCATGTC AATCATGTTA ATAGTGATAG GAGAGATTCC 
CTTCAGCAGA CCAATACCAC CCACCGTCCC CTGCATGTCC AAAGGCCTTC AATTCCACCT 
GCAAGTGATA CTGAGAAACC GCTGTTTCCT CCAGCAGGAA ATTCGGTGTG TCATAACCAT 
CATAACCATA ATTCCATAGG AAAGCAAGTT CCCACCTCAA CAAATGCCAA TCTCAATAAT 
GCCAATATGT CCAAAGCTGC CCATGGAAAG CGGCCCAGCA TTGGGAACCT TGAGCATGTG 
TCTGAAAATG GGCATCATTC TTCCCACAAG CATGACCGGG AGCCTCAGAG AAGGTCCAGT 
GTGAAAAGAA CCCGCTATTA TGAAACTTAC ATTAGGTCCG ACTCAGGAGA TGAACAGCTC 
CCAACTATTT GCCGGGAAGA CCCAGAGATA CATGGCTATT TCAGGGACCC CCACTGCTTG 
GGGGAGCAGG AGTATTTCAG TAGTGAGGAA TGCTACGAGG ATGACAGCTC GCCCACCTGG 
AGCAGGCAAA ACTATGGCTA CTACAGCAGA TACCCAGGCA GAAACATCGA CTCTGAGAGG 
CCCCGAGGCT ACCATCATCC CCAAGGATTC TTGGAGGACG ATGACTCGCC CGTTTGCTAT 
GATTCACGGA GATCTCCAAG GAGACGCCTA CTACCTCCCA CCCCAGCATC CCACCGGAGA 
TCCTCCTTCA ACTTTGAGTG CCTGCGCCGG CAGAGCAGCC AGGAAGAGGT CCCGTCGTCT 
CCCATCTTCC CCCATCGCAC GGCCCTGCCT CTGCATCTAA TGCAGCAACA GATCATGGCA 
GTTGCCGGCC TAGATTCAAG TAAAGCCCAG AAGTACTCAC CGAGTCACTC GACCCGGTCG 
TGGGCCACCC CTCCAGCAAC CCCTCCCTAC CGGGACTGGA CACCGTGCTA CACCCCCCTG 
ATCCAAGTGG AGCAGTCAGA GGCCCTGGAC CAGGTGAACG GCAGCCTGCC GTCCCTGCAC 
CGCAGCTCCT GGTACACAGA CGAGCCCGAC ATCTCCTACC GGACTTTCAC ACCAGCCAGC 
CTGACTGTCC CCAGCAGCTT CCGGAACAAA AACAGCGACA AGCAGAGGAG TGCGGACAGC 
TTGGTGGAGG CAGTCCTGAT ATCCGAAGGC TTGGGACGCT ATGCAAGGGA CCCAAAATTT 
GTGTCAGCAA CAAAACACGA AATCGCTGAT GCCTGTGACC TCACCATCGA CGAGATGGAG 
AGTGCAGCCA GCACCCTGCT TAATGGGAAC GTGCGTCCCC GAGCCAACGG GGATGTGGGC 
CCCCTCTCAC ACCGGCAGGA CTATGAGCTA CAGGACTTTG GTCCTGGCTA CAGCGACGAA 
GAGCCAGACC CTGGGAGGGA TGAGGAGGAC CTGGCGGATG AAATGATATG CATCACCACC 
TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( NM_001128840.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001122312.1 Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant 2, mRNA.

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CloneID	OHu10146
Clone ID Related Accession (Same CDS sequence)	NM_001128840.2 , NM_001128840.3
Accession Version	NM_001128840.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005905.1, M76558.1, M83566.1, AC132810.2, AB209171.1, AW008794.1 and AC012467.9. On Nov 22, 2018 this sequence version replaced NM_001128840.2. Transcript Variant: This variant (2) includes alternate in-frame exons, compared to variant 1, resulting in a shorter protein (isoform b), compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M76558.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145544, SAMEA2146411 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000350061.11/ ENSP00000288133.5 RefSeq Select criteria :: based on manual assertion, conservation ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTTCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACTGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTTTT CTGTTTATCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATAGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC AAGCGGCAAA TCTGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTTCGAG TGTTGCGACC ACTTCGACTA GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATCGTAGCT GAAGAGGACC CAGCTCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTAC TGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCG 
AACGGAGGCA TCACCAACTT TGATAACTTT GCCTTTGCCA TGCTTACTGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCAAA AGCACGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCTGAGGA CATCGATCCG GAGAATGAGG AAGAAGGAGG AGAGGAAGGC 
AAACGAAATA CTAGCATGCC CACCAGCGAG ACTGAGTCTG TGAACACAGA GAACGTCAGC 
GGTGAAGGCG AGAACCGAGG CTGCTGTGGA AGTCTCTGTC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGA TTCAATCGCA GAAGATGTAG GGCCGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATC GTCCTGGTGT TTCTGAACAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCAGATTGG TTGACACAGA TTCAAGATAT TGCCAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGCCTCCAA 
GCATATTTCG TCTCTCTTTT CAACCGGTTT GATTGCTTCG TGGTGTGTGG TGGAATCACT 
GAGACGATCT TGGTGGAACT GGAAATCATG TCTCCCCTGG GGATCTCTGT GTTTCGGTGT 
GTGCGCCTCT TAAGAATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCATCCTTAT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATTATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CACTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGACTG GAATGCTGTG ATGTACGATG GCATCATGGC TTACGGGGGC 
CCATCCTCTT CAGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA AGAAGCGGAA GAAAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTAG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA CTATAGAGAA GAGGATGAAG ACAAGGACCC CTATCCGCCT 
TGCGATGTGC CAGTAGGGGA AGAGGAAGAG GAAGAGGAGG AGGATGAACC TGAGGTTCCT 
GCCGGACCCC GTCCTCGAAG GATCTCGGAG TTGAACATGA AGGAAAAAAT TGCCCCCATC 
CCTGAAGGGA GCGCTTTCTT CATTCTTAGC AAGACCAACC CGATCCGCGT AGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC GATACTGGGT 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACTGTTGAGA TCCTGTTGAA GATGACAACT 
TTTGGAGCTT TCCTCCACAA AGGGGCCTTC TGCAGGAACT ACTTCAATTT GCTGGATATG 
CTGGTGGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGT CCCCTCAGGG CCATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG CGTCTTCGTG GCCATCCGGA CCATCGGCAA CATCATGATC 
GTCACCACCC TCCTGCAGTT CATGTTTGCC TGTATCGGGG TCCAGTTGTT CAAGGGGAAG 
TTCTATCGCT GTACGGATGA AGCCAAAAGT AACCCTGAAG AATGCAGGGG ACTTTTCATC 
CTCTACAAGG ATGGGGATGT TGACAGTCCT GTGGTCCGTG AACGGATCTG GCAAAACAGT 
GATTTCAACT TCGACAACGT CCTCTCTGCT ATGATGGCGC TCTTCACAGT CTCCACGTTT 
GAGGGCTGGC CTGCGTTGCT GTATAAAGCC ATCGACTCGA ATGGAGAGAA CATCGGCCCA 
ATCTACAACC ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTAGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTT GTCATCGTTA CATTTCAGGA ACAAGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CTTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACCGGGGT GTTCACCGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCAGAC CCAACTGAAA GTGAAAATGT CCCTGTCCCA 
ACTGCTACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGATTGGT GAAGCTTCTC AGCAGGGGGG AAGGCATCCG GACATTGCTG 
TGGACTTTTA TTAAGTCCTT TCAGGCGCTC CCGTATGTGG CCCTCCTCAT AGCCATGCTG 
TTCTTCATCT ATGCGGTCAT TGGCATGCAG ATGTTTGGGA AAGTTGCCAT GAGAGATAAC 
AACCAGATCA ATAGGAACAA TAACTTCCAG ACGTTTCCCC AGGCGGTGCT GCTGCTCTTC 
AGGTGTGCAA CAGGTGAGGC CTGGCAGGAG ATCATGCTGG CCTGTCTCCC AGGGAAGCTC 
TGTGACCCTG AGTCAGATTA CAACCCCGGG GAGGAGTATA CATGTGGGAG CAACTTTGCC 
ATTGTCTATT TCATCAGTTT TTACATGCTC TGTGCATTTC TGATCATCAA TCTGTTTGTG 
GCTGTCATCA TGGATAATTT CGACTATCTG ACCCGGGACT GGTCTATTTT GGGGCCTCAC 
CATTTAGATG AATTCAAAAG AATATGGTCA GAATATGACC CTGAGGCAAA GGGAAGGATA 
AAACACCTTG ATGTGGTCAC TCTGCTTCGA CGCATCCAGC CTCCCCTGGG GTTTGGGAAG 
TTATGTCCAC ACAGGGTAGC GTGCAAGAGA TTAGTTGCCA TGAACATGCC TCTCAACAGT 
GACGGGACAG TCATGTTTAA TGCAACCCTG TTTGCTTTGG TTCGAACGGC TCTTAAGATC 
AAGACCGAAG GGAACCTGGA GCAAGCTAAT GAAGAACTTC GGGCTGTGAT AAAGAAAATT 
TGGAAGAAAA CCAGCATGAA ATTACTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AACAAGGACT GGTGGGAAAG TACCCTGCGA AGAACACCAC AATTGCCCTA 
CAGGCGGGAT TAAGGACACT GCATGACATT GGGCCAGAAA TCCGGCGTGC TATATCGTGT 
GATTTGCAAG ATGACGAGCC TGAGGAAACA AAACGAGAAG AAGAAGATGA TGTGTTCAAA 
AGAAATGGTG CCCTGCTTGG AAACCATGTC AATCATGTTA ATAGTGATAG GAGAGATTCC 
CTTCAGCAGA CCAATACCAC CCACCGTCCC CTGCATGTCC AAAGGCCTTC AATTCCACCT 
GCAAGTGATA CTGAGAAACC GCTGTTTCCT CCAGCAGGAA ATTCGGTGTG TCATAACCAT 
CATAACCATA ATTCCATAGG AAAGCAAGTT CCCACCTCAA CAAATGCCAA TCTCAATAAT 
GCCAATATGT CCAAAGCTGC CCATGGAAAG CGGCCCAGCA TTGGGAACCT TGAGCATGTG 
TCTGAAAATG GGCATCATTC TTCCCACAAG CATGACCGGG AGCCTCAGAG AAGGTCCAGT 
GTGAAAAGAA CCCGCTATTA TGAAACTTAC ATTAGGTCCG ACTCAGGAGA TGAACAGCTC 
CCAACTATTT GCCGGGAAGA CCCAGAGATA CATGGCTATT TCAGGGACCC CCACTGCTTG 
GGGGAGCAGG AGTATTTCAG TAGTGAGGAA TGCTACGAGG ATGACAGCTC GCCCACCTGG 
AGCAGGCAAA ACTATGGCTA CTACAGCAGA TACCCAGGCA GAAACATCGA CTCTGAGAGG 
CCCCGAGGCT ACCATCATCC CCAAGGATTC TTGGAGGACG ATGACTCGCC CGTTTGCTAT 
GATTCACGGA GATCTCCAAG GAGACGCCTA CTACCTCCCA CCCCAGCATC CCACCGGAGA 
TCCTCCTTCA ACTTTGAGTG CCTGCGCCGG CAGAGCAGCC AGGAAGAGGT CCCGTCGTCT 
CCCATCTTCC CCCATCGCAC GGCCCTGCCT CTGCATCTAA TGCAGCAACA GATCATGGCA 
GTTGCCGGCC TAGATTCAAG TAAAGCCCAG AAGTACTCAC CGAGTCACTC GACCCGGTCG 
TGGGCCACCC CTCCAGCAAC CCCTCCCTAC CGGGACTGGA CACCGTGCTA CACCCCCCTG 
ATCCAAGTGG AGCAGTCAGA GGCCCTGGAC CAGGTGAACG GCAGCCTGCC GTCCCTGCAC 
CGCAGCTCCT GGTACACAGA CGAGCCCGAC ATCTCCTACC GGACTTTCAC ACCAGCCAGC 
CTGACTGTCC CCAGCAGCTT CCGGAACAAA AACAGCGACA AGCAGAGGAG TGCGGACAGC 
TTGGTGGAGG CAGTCCTGAT ATCCGAAGGC TTGGGACGCT ATGCAAGGGA CCCAAAATTT 
GTGTCAGCAA CAAAACACGA AATCGCTGAT GCCTGTGACC TCACCATCGA CGAGATGGAG 
AGTGCAGCCA GCACCCTGCT TAATGGGAAC GTGCGTCCCC GAGCCAACGG GGATGTGGGC 
CCCCTCTCAC ACCGGCAGGA CTATGAGCTA CAGGACTTTG GTCCTGGCTA CAGCGACGAA 
GAGCCAGACC CTGGGAGGGA TGAGGAGGAC CTGGCGGATG AAATGATATG CATCACCACC 
TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001122312.1
CDS	557..7042
Translation	MMMMMMMKKMQHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGENRGCCGSLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENIGPIYNHRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADPTESENVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEDDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGNLEHVSENGHHSSHKHDREPQRRSSVKRTRYYETYIRSDSGDEQLPTICREDPEIHGYFRDPHCLGEQEYFSSEECYEDDSSPTWSRQNYGYYSRYPGRNIDSERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPASHRRSSFNFECLRRQSSQEEVPSSPIFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVEQSEALDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGNVRPRANGDVGPLSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	NM_001128840.3
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128840.3

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTTCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACTGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTTTT CTGTTTATCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATAGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC AAGCGGCAAA TCTGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTTCGAG TGTTGCGACC ACTTCGACTA GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATCGTAGCT GAAGAGGACC CAGCTCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTAC TGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCG 
AACGGAGGCA TCACCAACTT TGATAACTTT GCCTTTGCCA TGCTTACTGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGATGA ATGATGCTAT GGGATTTGAA 
TTGCCCTGGG TGTATTTTGT CAGTCTCGTC ATCTTTGGGT CATTTTTCGT ACTAAATCTT 
GTACTTGGTG TATTGAGCGG AGAATTCTCA AAGGAAAGAG AGAAGGCAAA AGCACGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCTGAGGA CATCGATCCG GAGAATGAGG AAGAAGGAGG AGAGGAAGGC 
AAACGAAATA CTAGCATGCC CACCAGCGAG ACTGAGTCTG TGAACACAGA GAACGTCAGC 
GGTGAAGGCG AGAACCGAGG CTGCTGTGGA AGTCTCTGTC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGA TTCAATCGCA GAAGATGTAG GGCCGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATC GTCCTGGTGT TTCTGAACAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCAGATTGG TTGACACAGA TTCAAGATAT TGCCAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGCCTCCAA 
GCATATTTCG TCTCTCTTTT CAACCGGTTT GATTGCTTCG TGGTGTGTGG TGGAATCACT 
GAGACGATCT TGGTGGAACT GGAAATCATG TCTCCCCTGG GGATCTCTGT GTTTCGGTGT 
GTGCGCCTCT TAAGAATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCATCCTTAT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATTATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CACTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGACTG GAATGCTGTG ATGTACGATG GCATCATGGC TTACGGGGGC 
CCATCCTCTT CAGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA AGAAGCGGAA GAAAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTAG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA CTATAGAGAA GAGGATGAAG ACAAGGACCC CTATCCGCCT 
TGCGATGTGC CAGTAGGGGA AGAGGAAGAG GAAGAGGAGG AGGATGAACC TGAGGTTCCT 
GCCGGACCCC GTCCTCGAAG GATCTCGGAG TTGAACATGA AGGAAAAAAT TGCCCCCATC 
CCTGAAGGGA GCGCTTTCTT CATTCTTAGC AAGACCAACC CGATCCGCGT AGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC GATACTGGGT 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACTGTTGAGA TCCTGTTGAA GATGACAACT 
TTTGGAGCTT TCCTCCACAA AGGGGCCTTC TGCAGGAACT ACTTCAATTT GCTGGATATG 
CTGGTGGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGT CCCCTCAGGG CCATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG CGTCTTCGTG GCCATCCGGA CCATCGGCAA CATCATGATC 
GTCACCACCC TCCTGCAGTT CATGTTTGCC TGTATCGGGG TCCAGTTGTT CAAGGGGAAG 
TTCTATCGCT GTACGGATGA AGCCAAAAGT AACCCTGAAG AATGCAGGGG ACTTTTCATC 
CTCTACAAGG ATGGGGATGT TGACAGTCCT GTGGTCCGTG AACGGATCTG GCAAAACAGT 
GATTTCAACT TCGACAACGT CCTCTCTGCT ATGATGGCGC TCTTCACAGT CTCCACGTTT 
GAGGGCTGGC CTGCGTTGCT GTATAAAGCC ATCGACTCGA ATGGAGAGAA CATCGGCCCA 
ATCTACAACC ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTAGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTT GTCATCGTTA CATTTCAGGA ACAAGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CTTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACCGGGGT GTTCACCGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCAGAC CCAACTGAAA GTGAAAATGT CCCTGTCCCA 
ACTGCTACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGATTGGT GAAGCTTCTC AGCAGGGGGG AAGGCATCCG GACATTGCTG 
TGGACTTTTA TTAAGTCCTT TCAGGCGCTC CCGTATGTGG CCCTCCTCAT AGCCATGCTG 
TTCTTCATCT ATGCGGTCAT TGGCATGCAG ATGTTTGGGA AAGTTGCCAT GAGAGATAAC 
AACCAGATCA ATAGGAACAA TAACTTCCAG ACGTTTCCCC AGGCGGTGCT GCTGCTCTTC 
AGGTGTGCAA CAGGTGAGGC CTGGCAGGAG ATCATGCTGG CCTGTCTCCC AGGGAAGCTC 
TGTGACCCTG AGTCAGATTA CAACCCCGGG GAGGAGTATA CATGTGGGAG CAACTTTGCC 
ATTGTCTATT TCATCAGTTT TTACATGCTC TGTGCATTTC TGATCATCAA TCTGTTTGTG 
GCTGTCATCA TGGATAATTT CGACTATCTG ACCCGGGACT GGTCTATTTT GGGGCCTCAC 
CATTTAGATG AATTCAAAAG AATATGGTCA GAATATGACC CTGAGGCAAA GGGAAGGATA 
AAACACCTTG ATGTGGTCAC TCTGCTTCGA CGCATCCAGC CTCCCCTGGG GTTTGGGAAG 
TTATGTCCAC ACAGGGTAGC GTGCAAGAGA TTAGTTGCCA TGAACATGCC TCTCAACAGT 
GACGGGACAG TCATGTTTAA TGCAACCCTG TTTGCTTTGG TTCGAACGGC TCTTAAGATC 
AAGACCGAAG GGAACCTGGA GCAAGCTAAT GAAGAACTTC GGGCTGTGAT AAAGAAAATT 
TGGAAGAAAA CCAGCATGAA ATTACTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AACAAGGACT GGTGGGAAAG TACCCTGCGA AGAACACCAC AATTGCCCTA 
CAGGCGGGAT TAAGGACACT GCATGACATT GGGCCAGAAA TCCGGCGTGC TATATCGTGT 
GATTTGCAAG ATGACGAGCC TGAGGAAACA AAACGAGAAG AAGAAGATGA TGTGTTCAAA 
AGAAATGGTG CCCTGCTTGG AAACCATGTC AATCATGTTA ATAGTGATAG GAGAGATTCC 
CTTCAGCAGA CCAATACCAC CCACCGTCCC CTGCATGTCC AAAGGCCTTC AATTCCACCT 
GCAAGTGATA CTGAGAAACC GCTGTTTCCT CCAGCAGGAA ATTCGGTGTG TCATAACCAT 
CATAACCATA ATTCCATAGG AAAGCAAGTT CCCACCTCAA CAAATGCCAA TCTCAATAAT 
GCCAATATGT CCAAAGCTGC CCATGGAAAG CGGCCCAGCA TTGGGAACCT TGAGCATGTG 
TCTGAAAATG GGCATCATTC TTCCCACAAG CATGACCGGG AGCCTCAGAG AAGGTCCAGT 
GTGAAAAGAA CCCGCTATTA TGAAACTTAC ATTAGGTCCG ACTCAGGAGA TGAACAGCTC 
CCAACTATTT GCCGGGAAGA CCCAGAGATA CATGGCTATT TCAGGGACCC CCACTGCTTG 
GGGGAGCAGG AGTATTTCAG TAGTGAGGAA TGCTACGAGG ATGACAGCTC GCCCACCTGG 
AGCAGGCAAA ACTATGGCTA CTACAGCAGA TACCCAGGCA GAAACATCGA CTCTGAGAGG 
CCCCGAGGCT ACCATCATCC CCAAGGATTC TTGGAGGACG ATGACTCGCC CGTTTGCTAT 
GATTCACGGA GATCTCCAAG GAGACGCCTA CTACCTCCCA CCCCAGCATC CCACCGGAGA 
TCCTCCTTCA ACTTTGAGTG CCTGCGCCGG CAGAGCAGCC AGGAAGAGGT CCCGTCGTCT 
CCCATCTTCC CCCATCGCAC GGCCCTGCCT CTGCATCTAA TGCAGCAACA GATCATGGCA 
GTTGCCGGCC TAGATTCAAG TAAAGCCCAG AAGTACTCAC CGAGTCACTC GACCCGGTCG 
TGGGCCACCC CTCCAGCAAC CCCTCCCTAC CGGGACTGGA CACCGTGCTA CACCCCCCTG 
ATCCAAGTGG AGCAGTCAGA GGCCCTGGAC CAGGTGAACG GCAGCCTGCC GTCCCTGCAC 
CGCAGCTCCT GGTACACAGA CGAGCCCGAC ATCTCCTACC GGACTTTCAC ACCAGCCAGC 
CTGACTGTCC CCAGCAGCTT CCGGAACAAA AACAGCGACA AGCAGAGGAG TGCGGACAGC 
TTGGTGGAGG CAGTCCTGAT ATCCGAAGGC TTGGGACGCT ATGCAAGGGA CCCAAAATTT 
GTGTCAGCAA CAAAACACGA AATCGCTGAT GCCTGTGACC TCACCATCGA CGAGATGGAG 
AGTGCAGCCA GCACCCTGCT TAATGGGAAC GTGCGTCCCC GAGCCAACGG GGATGTGGGC 
CCCCTCTCAC ACCGGCAGGA CTATGAGCTA CAGGACTTTG GTCCTGGCTA CAGCGACGAA 
GAGCCAGACC CTGGGAGGGA TGAGGAGGAC CTGGCGGATG AAATGATATG CATCACCACC 
TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( NM_000720.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000711.1 Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant 1, mRNA.

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CloneID	OHu20038
Clone ID Related Accession (Same CDS sequence)	NM_000720.4 , NM_000720.3
Accession Version	NM_000720.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005905.1, M76558.1, M83566.1, AC132810.2, AB209171.1, AW008794.1 and AC012467.9. This sequence is a reference standard in the RefSeqGene project. On May 31, 2019 this sequence version replaced NM_000720.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M83566.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145544, SAMEA2162841 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTTCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACTGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTTTT CTGTTTATCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATAGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC AAGCGGCAAA TCTGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTTCGAG TGTTGCGACC ACTTCGACTA GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATCGTAGCT GAAGAGGACC CAGCTCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTAC TGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCG 
AACGGAGGCA TCACCAACTT TGATAACTTT GCCTTTGCCA TGCTTACTGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCA AAGGAAAGAG AGAAGGCAAA AGCACGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCTGAGGA CATCGATCCG GAGAATGAGG AAGAAGGAGG AGAGGAAGGC 
AAACGAAATA CTAGCATGCC CACCAGCGAG ACTGAGTCTG TGAACACAGA GAACGTCAGC 
GGTGAAGGCG AGAACCGAGG CTGCTGTGGA AGTCTCTGGT GCTGGTGGAG ACGGAGAGGC 
GCGGCCAAGG CGGGGCCCTC TGGGTGTCGG CGGTGGGGTC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGA TTCAATCGCA GAAGATGTAG GGCCGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATC GTCCTGGTGT TTCTGAACAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCAGATTGG TTGACACAGA TTCAAGATAT TGCCAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGCCTCCAA 
GCATATTTCG TCTCTCTTTT CAACCGGTTT GATTGCTTCG TGGTGTGTGG TGGAATCACT 
GAGACGATCT TGGTGGAACT GGAAATCATG TCTCCCCTGG GGATCTCTGT GTTTCGGTGT 
GTGCGCCTCT TAAGAATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCATCCTTAT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATTATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CACTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGACTG GAATGCTGTG ATGTACGATG GCATCATGGC TTACGGGGGC 
CCATCCTCTT CAGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA AGAAGCGGAA GAAAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTAG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA CTATAGAGAA GAGGATGAAG ACAAGGACCC CTATCCGCCT 
TGCGATGTGC CAGTAGGGGA AGAGGAAGAG GAAGAGGAGG AGGATGAACC TGAGGTTCCT 
GCCGGACCCC GTCCTCGAAG GATCTCGGAG TTGAACATGA AGGAAAAAAT TGCCCCCATC 
CCTGAAGGGA GCGCTTTCTT CATTCTTAGC AAGACCAACC CGATCCGCGT AGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC GATACTGGGT 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACTGTTGAGA TCCTGTTGAA GATGACAACT 
TTTGGAGCTT TCCTCCACAA AGGGGCCTTC TGCAGGAACT ACTTCAATTT GCTGGATATG 
CTGGTGGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGT CCCCTCAGGG CCATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG CGTCTTCGTG GCCATCCGGA CCATCGGCAA CATCATGATC 
GTCACCACCC TCCTGCAGTT CATGTTTGCC TGTATCGGGG TCCAGTTGTT CAAGGGGAAG 
TTCTATCGCT GTACGGATGA AGCCAAAAGT AACCCTGAAG AATGCAGGGG ACTTTTCATC 
CTCTACAAGG ATGGGGATGT TGACAGTCCT GTGGTCCGTG AACGGATCTG GCAAAACAGT 
GATTTCAACT TCGACAACGT CCTCTCTGCT ATGATGGCGC TCTTCACAGT CTCCACGTTT 
GAGGGCTGGC CTGCGTTGCT GTATAAAGCC ATCGACTCGA ATGGAGAGAA CATCGGCCCA 
ATCTACAACC ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTAGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTT GTCATCGTTA CATTTCAGGA ACAAGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CTTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACCGGGGT GTTCACCGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCAGAC CCAACTGAAA GTGAAAATGT CCCTGTCCCA 
ACTGCTACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGATTGGT GAAGCTTCTC AGCAGGGGGG AAGGCATCCG GACATTGCTG 
TGGACTTTTA TTAAGTCCTT TCAGGCGCTC CCGTATGTGG CCCTCCTCAT AGCCATGCTG 
TTCTTCATCT ATGCGGTCAT TGGCATGCAG ATGTTTGGGA AAGTTGCCAT GAGAGATAAC 
AACCAGATCA ATAGGAACAA TAACTTCCAG ACGTTTCCCC AGGCGGTGCT GCTGCTCTTC 
AGGTGTGCAA CAGGTGAGGC CTGGCAGGAG ATCATGCTGG CCTGTCTCCC AGGGAAGCTC 
TGTGACCCTG AGTCAGATTA CAACCCCGGG GAGGAGTATA CATGTGGGAG CAACTTTGCC 
ATTGTCTATT TCATCAGTTT TTACATGCTC TGTGCATTTC TGATCATCAA TCTGTTTGTG 
GCTGTCATCA TGGATAATTT CGACTATCTG ACCCGGGACT GGTCTATTTT GGGGCCTCAC 
CATTTAGATG AATTCAAAAG AATATGGTCA GAATATGACC CTGAGGCAAA GGGAAGGATA 
AAACACCTTG ATGTGGTCAC TCTGCTTCGA CGCATCCAGC CTCCCCTGGG GTTTGGGAAG 
TTATGTCCAC ACAGGGTAGC GTGCAAGAGA TTAGTTGCCA TGAACATGCC TCTCAACAGT 
GACGGGACAG TCATGTTTAA TGCAACCCTG TTTGCTTTGG TTCGAACGGC TCTTAAGATC 
AAGACCGAAG GGAACCTGGA GCAAGCTAAT GAAGAACTTC GGGCTGTGAT AAAGAAAATT 
TGGAAGAAAA CCAGCATGAA ATTACTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AACAAGGACT GGTGGGAAAG TACCCTGCGA AGAACACCAC AATTGCCCTA 
CAGGCGGGAT TAAGGACACT GCATGACATT GGGCCAGAAA TCCGGCGTGC TATATCGTGT 
GATTTGCAAG ATGACGAGCC TGAGGAAACA AAACGAGAAG AAGAAGATGA TGTGTTCAAA 
AGAAATGGTG CCCTGCTTGG AAACCATGTC AATCATGTTA ATAGTGATAG GAGAGATTCC 
CTTCAGCAGA CCAATACCAC CCACCGTCCC CTGCATGTCC AAAGGCCTTC AATTCCACCT 
GCAAGTGATA CTGAGAAACC GCTGTTTCCT CCAGCAGGAA ATTCGGTGTG TCATAACCAT 
CATAACCATA ATTCCATAGG AAAGCAAGTT CCCACCTCAA CAAATGCCAA TCTCAATAAT 
GCCAATATGT CCAAAGCTGC CCATGGAAAG CGGCCCAGCA TTGGGAACCT TGAGCATGTG 
TCTGAAAATG GGCATCATTC TTCCCACAAG CATGACCGGG AGCCTCAGAG AAGGTCCAGT 
GTGAAAAGAA CCCGCTATTA TGAAACTTAC ATTAGGTCCG ACTCAGGAGA TGAACAGCTC 
CCAACTATTT GCCGGGAAGA CCCAGAGATA CATGGCTATT TCAGGGACCC CCACTGCTTG 
GGGGAGCAGG AGTATTTCAG TAGTGAGGAA TGCTACGAGG ATGACAGCTC GCCCACCTGG 
AGCAGGCAAA ACTATGGCTA CTACAGCAGA TACCCAGGCA GAAACATCGA CTCTGAGAGG 
CCCCGAGGCT ACCATCATCC CCAAGGATTC TTGGAGGACG ATGACTCGCC CGTTTGCTAT 
GATTCACGGA GATCTCCAAG GAGACGCCTA CTACCTCCCA CCCCAGCATC CCACCGGAGA 
TCCTCCTTCA ACTTTGAGTG CCTGCGCCGG CAGAGCAGCC AGGAAGAGGT CCCGTCGTCT 
CCCATCTTCC CCCATCGCAC GGCCCTGCCT CTGCATCTAA TGCAGCAACA GATCATGGCA 
GTTGCCGGCC TAGATTCAAG TAAAGCCCAG AAGTACTCAC CGAGTCACTC GACCCGGTCG 
TGGGCCACCC CTCCAGCAAC CCCTCCCTAC CGGGACTGGA CACCGTGCTA CACCCCCCTG 
ATCCAAGTGG AGCAGTCAGA GGCCCTGGAC CAGGTGAACG GCAGCCTGCC GTCCCTGCAC 
CGCAGCTCCT GGTACACAGA CGAGCCCGAC ATCTCCTACC GGACTTTCAC ACCAGCCAGC 
CTGACTGTCC CCAGCAGCTT CCGGAACAAA AACAGCGACA AGCAGAGGAG TGCGGACAGC 
TTGGTGGAGG CAGTCCTGAT ATCCGAAGGC TTGGGACGCT ATGCAAGGGA CCCAAAATTT 
GTGTCAGCAA CAAAACACGA AATCGCTGAT GCCTGTGACC TCACCATCGA CGAGATGGAG 
AGTGCAGCCA GCACCCTGCT TAATGGGAAC GTGCGTCCCC GAGCCAACGG GGATGTGGGC 
CCCCTCTCAC ACCGGCAGGA CTATGAGCTA CAGGACTTTG GTCCTGGCTA CAGCGACGAA 
GAGCCAGACC CTGGGAGGGA TGAGGAGGAC CTGGCGGATG AAATGATATG CATCACCACC 
TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000711.1
CDS	557..7102
Translation	MMMMMMMKKMQHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGENRGCCGSLWCWWRRRGAAKAGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENIGPIYNHRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADPTESENVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEDDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGNLEHVSENGHHSSHKHDREPQRRSSVKRTRYYETYIRSDSGDEQLPTICREDPEIHGYFRDPHCLGEQEYFSSEECYEDDSSPTWSRQNYGYYSRYPGRNIDSERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPASHRRSSFNFECLRRQSSQEEVPSSPIFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVEQSEALDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGNVRPRANGDVGPLSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	NM_000720.4
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000720.4

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTTCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACTGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTTTT CTGTTTATCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATAGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC AAGCGGCAAA TCTGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTTCGAG TGTTGCGACC ACTTCGACTA GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATCGTAGCT GAAGAGGACC CAGCTCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTAC TGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCG 
AACGGAGGCA TCACCAACTT TGATAACTTT GCCTTTGCCA TGCTTACTGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCA AAGGAAAGAG AGAAGGCAAA AGCACGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCTGAGGA CATCGATCCG GAGAATGAGG AAGAAGGAGG AGAGGAAGGC 
AAACGAAATA CTAGCATGCC CACCAGCGAG ACTGAGTCTG TGAACACAGA GAACGTCAGC 
GGTGAAGGCG AGAACCGAGG CTGCTGTGGA AGTCTCTGGT GCTGGTGGAG ACGGAGAGGC 
GCGGCCAAGG CGGGGCCCTC TGGGTGTCGG CGGTGGGGTC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGA TTCAATCGCA GAAGATGTAG GGCCGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATC GTCCTGGTGT TTCTGAACAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCAGATTGG TTGACACAGA TTCAAGATAT TGCCAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGCCTCCAA 
GCATATTTCG TCTCTCTTTT CAACCGGTTT GATTGCTTCG TGGTGTGTGG TGGAATCACT 
GAGACGATCT TGGTGGAACT GGAAATCATG TCTCCCCTGG GGATCTCTGT GTTTCGGTGT 
GTGCGCCTCT TAAGAATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCATCCTTAT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATTATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CACTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGACTG GAATGCTGTG ATGTACGATG GCATCATGGC TTACGGGGGC 
CCATCCTCTT CAGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA AGAAGCGGAA GAAAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTAG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA CTATAGAGAA GAGGATGAAG ACAAGGACCC CTATCCGCCT 
TGCGATGTGC CAGTAGGGGA AGAGGAAGAG GAAGAGGAGG AGGATGAACC TGAGGTTCCT 
GCCGGACCCC GTCCTCGAAG GATCTCGGAG TTGAACATGA AGGAAAAAAT TGCCCCCATC 
CCTGAAGGGA GCGCTTTCTT CATTCTTAGC AAGACCAACC CGATCCGCGT AGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC GATACTGGGT 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACTGTTGAGA TCCTGTTGAA GATGACAACT 
TTTGGAGCTT TCCTCCACAA AGGGGCCTTC TGCAGGAACT ACTTCAATTT GCTGGATATG 
CTGGTGGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGT CCCCTCAGGG CCATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG CGTCTTCGTG GCCATCCGGA CCATCGGCAA CATCATGATC 
GTCACCACCC TCCTGCAGTT CATGTTTGCC TGTATCGGGG TCCAGTTGTT CAAGGGGAAG 
TTCTATCGCT GTACGGATGA AGCCAAAAGT AACCCTGAAG AATGCAGGGG ACTTTTCATC 
CTCTACAAGG ATGGGGATGT TGACAGTCCT GTGGTCCGTG AACGGATCTG GCAAAACAGT 
GATTTCAACT TCGACAACGT CCTCTCTGCT ATGATGGCGC TCTTCACAGT CTCCACGTTT 
GAGGGCTGGC CTGCGTTGCT GTATAAAGCC ATCGACTCGA ATGGAGAGAA CATCGGCCCA 
ATCTACAACC ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTAGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTT GTCATCGTTA CATTTCAGGA ACAAGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CTTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACCGGGGT GTTCACCGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCAGAC CCAACTGAAA GTGAAAATGT CCCTGTCCCA 
ACTGCTACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGATTGGT GAAGCTTCTC AGCAGGGGGG AAGGCATCCG GACATTGCTG 
TGGACTTTTA TTAAGTCCTT TCAGGCGCTC CCGTATGTGG CCCTCCTCAT AGCCATGCTG 
TTCTTCATCT ATGCGGTCAT TGGCATGCAG ATGTTTGGGA AAGTTGCCAT GAGAGATAAC 
AACCAGATCA ATAGGAACAA TAACTTCCAG ACGTTTCCCC AGGCGGTGCT GCTGCTCTTC 
AGGTGTGCAA CAGGTGAGGC CTGGCAGGAG ATCATGCTGG CCTGTCTCCC AGGGAAGCTC 
TGTGACCCTG AGTCAGATTA CAACCCCGGG GAGGAGTATA CATGTGGGAG CAACTTTGCC 
ATTGTCTATT TCATCAGTTT TTACATGCTC TGTGCATTTC TGATCATCAA TCTGTTTGTG 
GCTGTCATCA TGGATAATTT CGACTATCTG ACCCGGGACT GGTCTATTTT GGGGCCTCAC 
CATTTAGATG AATTCAAAAG AATATGGTCA GAATATGACC CTGAGGCAAA GGGAAGGATA 
AAACACCTTG ATGTGGTCAC TCTGCTTCGA CGCATCCAGC CTCCCCTGGG GTTTGGGAAG 
TTATGTCCAC ACAGGGTAGC GTGCAAGAGA TTAGTTGCCA TGAACATGCC TCTCAACAGT 
GACGGGACAG TCATGTTTAA TGCAACCCTG TTTGCTTTGG TTCGAACGGC TCTTAAGATC 
AAGACCGAAG GGAACCTGGA GCAAGCTAAT GAAGAACTTC GGGCTGTGAT AAAGAAAATT 
TGGAAGAAAA CCAGCATGAA ATTACTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AACAAGGACT GGTGGGAAAG TACCCTGCGA AGAACACCAC AATTGCCCTA 
CAGGCGGGAT TAAGGACACT GCATGACATT GGGCCAGAAA TCCGGCGTGC TATATCGTGT 
GATTTGCAAG ATGACGAGCC TGAGGAAACA AAACGAGAAG AAGAAGATGA TGTGTTCAAA 
AGAAATGGTG CCCTGCTTGG AAACCATGTC AATCATGTTA ATAGTGATAG GAGAGATTCC 
CTTCAGCAGA CCAATACCAC CCACCGTCCC CTGCATGTCC AAAGGCCTTC AATTCCACCT 
GCAAGTGATA CTGAGAAACC GCTGTTTCCT CCAGCAGGAA ATTCGGTGTG TCATAACCAT 
CATAACCATA ATTCCATAGG AAAGCAAGTT CCCACCTCAA CAAATGCCAA TCTCAATAAT 
GCCAATATGT CCAAAGCTGC CCATGGAAAG CGGCCCAGCA TTGGGAACCT TGAGCATGTG 
TCTGAAAATG GGCATCATTC TTCCCACAAG CATGACCGGG AGCCTCAGAG AAGGTCCAGT 
GTGAAAAGAA CCCGCTATTA TGAAACTTAC ATTAGGTCCG ACTCAGGAGA TGAACAGCTC 
CCAACTATTT GCCGGGAAGA CCCAGAGATA CATGGCTATT TCAGGGACCC CCACTGCTTG 
GGGGAGCAGG AGTATTTCAG TAGTGAGGAA TGCTACGAGG ATGACAGCTC GCCCACCTGG 
AGCAGGCAAA ACTATGGCTA CTACAGCAGA TACCCAGGCA GAAACATCGA CTCTGAGAGG 
CCCCGAGGCT ACCATCATCC CCAAGGATTC TTGGAGGACG ATGACTCGCC CGTTTGCTAT 
GATTCACGGA GATCTCCAAG GAGACGCCTA CTACCTCCCA CCCCAGCATC CCACCGGAGA 
TCCTCCTTCA ACTTTGAGTG CCTGCGCCGG CAGAGCAGCC AGGAAGAGGT CCCGTCGTCT 
CCCATCTTCC CCCATCGCAC GGCCCTGCCT CTGCATCTAA TGCAGCAACA GATCATGGCA 
GTTGCCGGCC TAGATTCAAG TAAAGCCCAG AAGTACTCAC CGAGTCACTC GACCCGGTCG 
TGGGCCACCC CTCCAGCAAC CCCTCCCTAC CGGGACTGGA CACCGTGCTA CACCCCCCTG 
ATCCAAGTGG AGCAGTCAGA GGCCCTGGAC CAGGTGAACG GCAGCCTGCC GTCCCTGCAC 
CGCAGCTCCT GGTACACAGA CGAGCCCGAC ATCTCCTACC GGACTTTCAC ACCAGCCAGC 
CTGACTGTCC CCAGCAGCTT CCGGAACAAA AACAGCGACA AGCAGAGGAG TGCGGACAGC 
TTGGTGGAGG CAGTCCTGAT ATCCGAAGGC TTGGGACGCT ATGCAAGGGA CCCAAAATTT 
GTGTCAGCAA CAAAACACGA AATCGCTGAT GCCTGTGACC TCACCATCGA CGAGATGGAG 
AGTGCAGCCA GCACCCTGCT TAATGGGAAC GTGCGTCCCC GAGCCAACGG GGATGTGGGC 
CCCCTCTCAC ACCGGCAGGA CTATGAGCTA CAGGACTTTG GTCCTGGCTA CAGCGACGAA 
GAGCCAGACC CTGGGAGGGA TGAGGAGGAC CTGGCGGATG AAATGATATG CATCACCACC 
TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( NM_000720.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000711.1 Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant 1, mRNA.

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CloneID	OHu20038
Clone ID Related Accession (Same CDS sequence)	NM_000720.4 , NM_000720.3
Accession Version	NM_000720.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M76558.1, M83566.1, AC132810.2, AB209171.1 and AW008794.1. This sequence is a reference standard in the RefSeqGene project. On Dec 4, 2012 this sequence version replaced NM_000720.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M83566.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145544, SAMEA2162841 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTTCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACTGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTTTT CTGTTTATCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATAGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC AAGCGGCAAA TCTGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTTCGAG TGTTGCGACC ACTTCGACTA GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATCGTAGCT GAAGAGGACC CAGCTCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTAC TGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCG 
AACGGAGGCA TCACCAACTT TGATAACTTT GCCTTTGCCA TGCTTACTGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCA AAGGAAAGAG AGAAGGCAAA AGCACGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCTGAGGA CATCGATCCG GAGAATGAGG AAGAAGGAGG AGAGGAAGGC 
AAACGAAATA CTAGCATGCC CACCAGCGAG ACTGAGTCTG TGAACACAGA GAACGTCAGC 
GGTGAAGGCG AGAACCGAGG CTGCTGTGGA AGTCTCTGGT GCTGGTGGAG ACGGAGAGGC 
GCGGCCAAGG CGGGGCCCTC TGGGTGTCGG CGGTGGGGTC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGA TTCAATCGCA GAAGATGTAG GGCCGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATC GTCCTGGTGT TTCTGAACAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCAGATTGG TTGACACAGA TTCAAGATAT TGCCAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGCCTCCAA 
GCATATTTCG TCTCTCTTTT CAACCGGTTT GATTGCTTCG TGGTGTGTGG TGGAATCACT 
GAGACGATCT TGGTGGAACT GGAAATCATG TCTCCCCTGG GGATCTCTGT GTTTCGGTGT 
GTGCGCCTCT TAAGAATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCATCCTTAT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATTATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CACTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGACTG GAATGCTGTG ATGTACGATG GCATCATGGC TTACGGGGGC 
CCATCCTCTT CAGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA AGAAGCGGAA GAAAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTAG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA CTATAGAGAA GAGGATGAAG ACAAGGACCC CTATCCGCCT 
TGCGATGTGC CAGTAGGGGA AGAGGAAGAG GAAGAGGAGG AGGATGAACC TGAGGTTCCT 
GCCGGACCCC GTCCTCGAAG GATCTCGGAG TTGAACATGA AGGAAAAAAT TGCCCCCATC 
CCTGAAGGGA GCGCTTTCTT CATTCTTAGC AAGACCAACC CGATCCGCGT AGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC GATACTGGGT 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACTGTTGAGA TCCTGTTGAA GATGACAACT 
TTTGGAGCTT TCCTCCACAA AGGGGCCTTC TGCAGGAACT ACTTCAATTT GCTGGATATG 
CTGGTGGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGT CCCCTCAGGG CCATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG CGTCTTCGTG GCCATCCGGA CCATCGGCAA CATCATGATC 
GTCACCACCC TCCTGCAGTT CATGTTTGCC TGTATCGGGG TCCAGTTGTT CAAGGGGAAG 
TTCTATCGCT GTACGGATGA AGCCAAAAGT AACCCTGAAG AATGCAGGGG ACTTTTCATC 
CTCTACAAGG ATGGGGATGT TGACAGTCCT GTGGTCCGTG AACGGATCTG GCAAAACAGT 
GATTTCAACT TCGACAACGT CCTCTCTGCT ATGATGGCGC TCTTCACAGT CTCCACGTTT 
GAGGGCTGGC CTGCGTTGCT GTATAAAGCC ATCGACTCGA ATGGAGAGAA CATCGGCCCA 
ATCTACAACC ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTAGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTT GTCATCGTTA CATTTCAGGA ACAAGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CTTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACCGGGGT GTTCACCGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCAGAC CCAACTGAAA GTGAAAATGT CCCTGTCCCA 
ACTGCTACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGATTGGT GAAGCTTCTC AGCAGGGGGG AAGGCATCCG GACATTGCTG 
TGGACTTTTA TTAAGTCCTT TCAGGCGCTC CCGTATGTGG CCCTCCTCAT AGCCATGCTG 
TTCTTCATCT ATGCGGTCAT TGGCATGCAG ATGTTTGGGA AAGTTGCCAT GAGAGATAAC 
AACCAGATCA ATAGGAACAA TAACTTCCAG ACGTTTCCCC AGGCGGTGCT GCTGCTCTTC 
AGGTGTGCAA CAGGTGAGGC CTGGCAGGAG ATCATGCTGG CCTGTCTCCC AGGGAAGCTC 
TGTGACCCTG AGTCAGATTA CAACCCCGGG GAGGAGTATA CATGTGGGAG CAACTTTGCC 
ATTGTCTATT TCATCAGTTT TTACATGCTC TGTGCATTTC TGATCATCAA TCTGTTTGTG 
GCTGTCATCA TGGATAATTT CGACTATCTG ACCCGGGACT GGTCTATTTT GGGGCCTCAC 
CATTTAGATG AATTCAAAAG AATATGGTCA GAATATGACC CTGAGGCAAA GGGAAGGATA 
AAACACCTTG ATGTGGTCAC TCTGCTTCGA CGCATCCAGC CTCCCCTGGG GTTTGGGAAG 
TTATGTCCAC ACAGGGTAGC GTGCAAGAGA TTAGTTGCCA TGAACATGCC TCTCAACAGT 
GACGGGACAG TCATGTTTAA TGCAACCCTG TTTGCTTTGG TTCGAACGGC TCTTAAGATC 
AAGACCGAAG GGAACCTGGA GCAAGCTAAT GAAGAACTTC GGGCTGTGAT AAAGAAAATT 
TGGAAGAAAA CCAGCATGAA ATTACTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AACAAGGACT GGTGGGAAAG TACCCTGCGA AGAACACCAC AATTGCCCTA 
CAGGCGGGAT TAAGGACACT GCATGACATT GGGCCAGAAA TCCGGCGTGC TATATCGTGT 
GATTTGCAAG ATGACGAGCC TGAGGAAACA AAACGAGAAG AAGAAGATGA TGTGTTCAAA 
AGAAATGGTG CCCTGCTTGG AAACCATGTC AATCATGTTA ATAGTGATAG GAGAGATTCC 
CTTCAGCAGA CCAATACCAC CCACCGTCCC CTGCATGTCC AAAGGCCTTC AATTCCACCT 
GCAAGTGATA CTGAGAAACC GCTGTTTCCT CCAGCAGGAA ATTCGGTGTG TCATAACCAT 
CATAACCATA ATTCCATAGG AAAGCAAGTT CCCACCTCAA CAAATGCCAA TCTCAATAAT 
GCCAATATGT CCAAAGCTGC CCATGGAAAG CGGCCCAGCA TTGGGAACCT TGAGCATGTG 
TCTGAAAATG GGCATCATTC TTCCCACAAG CATGACCGGG AGCCTCAGAG AAGGTCCAGT 
GTGAAAAGAA CCCGCTATTA TGAAACTTAC ATTAGGTCCG ACTCAGGAGA TGAACAGCTC 
CCAACTATTT GCCGGGAAGA CCCAGAGATA CATGGCTATT TCAGGGACCC CCACTGCTTG 
GGGGAGCAGG AGTATTTCAG TAGTGAGGAA TGCTACGAGG ATGACAGCTC GCCCACCTGG 
AGCAGGCAAA ACTATGGCTA CTACAGCAGA TACCCAGGCA GAAACATCGA CTCTGAGAGG 
CCCCGAGGCT ACCATCATCC CCAAGGATTC TTGGAGGACG ATGACTCGCC CGTTTGCTAT 
GATTCACGGA GATCTCCAAG GAGACGCCTA CTACCTCCCA CCCCAGCATC CCACCGGAGA 
TCCTCCTTCA ACTTTGAGTG CCTGCGCCGG CAGAGCAGCC AGGAAGAGGT CCCGTCGTCT 
CCCATCTTCC CCCATCGCAC GGCCCTGCCT CTGCATCTAA TGCAGCAACA GATCATGGCA 
GTTGCCGGCC TAGATTCAAG TAAAGCCCAG AAGTACTCAC CGAGTCACTC GACCCGGTCG 
TGGGCCACCC CTCCAGCAAC CCCTCCCTAC CGGGACTGGA CACCGTGCTA CACCCCCCTG 
ATCCAAGTGG AGCAGTCAGA GGCCCTGGAC CAGGTGAACG GCAGCCTGCC GTCCCTGCAC 
CGCAGCTCCT GGTACACAGA CGAGCCCGAC ATCTCCTACC GGACTTTCAC ACCAGCCAGC 
CTGACTGTCC CCAGCAGCTT CCGGAACAAA AACAGCGACA AGCAGAGGAG TGCGGACAGC 
TTGGTGGAGG CAGTCCTGAT ATCCGAAGGC TTGGGACGCT ATGCAAGGGA CCCAAAATTT 
GTGTCAGCAA CAAAACACGA AATCGCTGAT GCCTGTGACC TCACCATCGA CGAGATGGAG 
AGTGCAGCCA GCACCCTGCT TAATGGGAAC GTGCGTCCCC GAGCCAACGG GGATGTGGGC 
CCCCTCTCAC ACCGGCAGGA CTATGAGCTA CAGGACTTTG GTCCTGGCTA CAGCGACGAA 
GAGCCAGACC CTGGGAGGGA TGAGGAGGAC CTGGCGGATG AAATGATATG CATCACCACC 
TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000711.1
CDS	119..6664
Translation	MMMMMMMKKMQHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGENRGCCGSLWCWWRRRGAAKAGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENIGPIYNHRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADPTESENVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEDDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGNLEHVSENGHHSSHKHDREPQRRSSVKRTRYYETYIRSDSGDEQLPTICREDPEIHGYFRDPHCLGEQEYFSSEECYEDDSSPTWSRQNYGYYSRYPGRNIDSERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPASHRRSSFNFECLRRQSSQEEVPSSPIFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVEQSEALDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGNVRPRANGDVGPLSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	NM_000720.3
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000720.3

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTTCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACTGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTTTT CTGTTTATCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATAGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC AAGCGGCAAA TCTGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTTCGAG TGTTGCGACC ACTTCGACTA GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATCGTAGCT GAAGAGGACC CAGCTCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTAC TGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCG 
AACGGAGGCA TCACCAACTT TGATAACTTT GCCTTTGCCA TGCTTACTGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCA AAGGAAAGAG AGAAGGCAAA AGCACGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCTGAGGA CATCGATCCG GAGAATGAGG AAGAAGGAGG AGAGGAAGGC 
AAACGAAATA CTAGCATGCC CACCAGCGAG ACTGAGTCTG TGAACACAGA GAACGTCAGC 
GGTGAAGGCG AGAACCGAGG CTGCTGTGGA AGTCTCTGGT GCTGGTGGAG ACGGAGAGGC 
GCGGCCAAGG CGGGGCCCTC TGGGTGTCGG CGGTGGGGTC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGA TTCAATCGCA GAAGATGTAG GGCCGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATC GTCCTGGTGT TTCTGAACAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCAGATTGG TTGACACAGA TTCAAGATAT TGCCAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGCCTCCAA 
GCATATTTCG TCTCTCTTTT CAACCGGTTT GATTGCTTCG TGGTGTGTGG TGGAATCACT 
GAGACGATCT TGGTGGAACT GGAAATCATG TCTCCCCTGG GGATCTCTGT GTTTCGGTGT 
GTGCGCCTCT TAAGAATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCATCCTTAT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATTATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CACTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGACTG GAATGCTGTG ATGTACGATG GCATCATGGC TTACGGGGGC 
CCATCCTCTT CAGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA AGAAGCGGAA GAAAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTAG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA CTATAGAGAA GAGGATGAAG ACAAGGACCC CTATCCGCCT 
TGCGATGTGC CAGTAGGGGA AGAGGAAGAG GAAGAGGAGG AGGATGAACC TGAGGTTCCT 
GCCGGACCCC GTCCTCGAAG GATCTCGGAG TTGAACATGA AGGAAAAAAT TGCCCCCATC 
CCTGAAGGGA GCGCTTTCTT CATTCTTAGC AAGACCAACC CGATCCGCGT AGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC GATACTGGGT 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACTGTTGAGA TCCTGTTGAA GATGACAACT 
TTTGGAGCTT TCCTCCACAA AGGGGCCTTC TGCAGGAACT ACTTCAATTT GCTGGATATG 
CTGGTGGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGT CCCCTCAGGG CCATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG CGTCTTCGTG GCCATCCGGA CCATCGGCAA CATCATGATC 
GTCACCACCC TCCTGCAGTT CATGTTTGCC TGTATCGGGG TCCAGTTGTT CAAGGGGAAG 
TTCTATCGCT GTACGGATGA AGCCAAAAGT AACCCTGAAG AATGCAGGGG ACTTTTCATC 
CTCTACAAGG ATGGGGATGT TGACAGTCCT GTGGTCCGTG AACGGATCTG GCAAAACAGT 
GATTTCAACT TCGACAACGT CCTCTCTGCT ATGATGGCGC TCTTCACAGT CTCCACGTTT 
GAGGGCTGGC CTGCGTTGCT GTATAAAGCC ATCGACTCGA ATGGAGAGAA CATCGGCCCA 
ATCTACAACC ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTAGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTT GTCATCGTTA CATTTCAGGA ACAAGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CTTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACCGGGGT GTTCACCGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCAGAC CCAACTGAAA GTGAAAATGT CCCTGTCCCA 
ACTGCTACAC CTGGGAACTC TGAAGAGAGC AATAGAATCT CCATCACCTT TTTCCGTCTT 
TTCCGAGTGA TGCGATTGGT GAAGCTTCTC AGCAGGGGGG AAGGCATCCG GACATTGCTG 
TGGACTTTTA TTAAGTCCTT TCAGGCGCTC CCGTATGTGG CCCTCCTCAT AGCCATGCTG 
TTCTTCATCT ATGCGGTCAT TGGCATGCAG ATGTTTGGGA AAGTTGCCAT GAGAGATAAC 
AACCAGATCA ATAGGAACAA TAACTTCCAG ACGTTTCCCC AGGCGGTGCT GCTGCTCTTC 
AGGTGTGCAA CAGGTGAGGC CTGGCAGGAG ATCATGCTGG CCTGTCTCCC AGGGAAGCTC 
TGTGACCCTG AGTCAGATTA CAACCCCGGG GAGGAGTATA CATGTGGGAG CAACTTTGCC 
ATTGTCTATT TCATCAGTTT TTACATGCTC TGTGCATTTC TGATCATCAA TCTGTTTGTG 
GCTGTCATCA TGGATAATTT CGACTATCTG ACCCGGGACT GGTCTATTTT GGGGCCTCAC 
CATTTAGATG AATTCAAAAG AATATGGTCA GAATATGACC CTGAGGCAAA GGGAAGGATA 
AAACACCTTG ATGTGGTCAC TCTGCTTCGA CGCATCCAGC CTCCCCTGGG GTTTGGGAAG 
TTATGTCCAC ACAGGGTAGC GTGCAAGAGA TTAGTTGCCA TGAACATGCC TCTCAACAGT 
GACGGGACAG TCATGTTTAA TGCAACCCTG TTTGCTTTGG TTCGAACGGC TCTTAAGATC 
AAGACCGAAG GGAACCTGGA GCAAGCTAAT GAAGAACTTC GGGCTGTGAT AAAGAAAATT 
TGGAAGAAAA CCAGCATGAA ATTACTTGAC CAAGTTGTCC CTCCAGCTGG TGATGATGAG 
GTAACCGTGG GGAAGTTCTA TGCCACTTTC CTGATACAGG ACTACTTTAG GAAATTCAAG 
AAACGGAAAG AACAAGGACT GGTGGGAAAG TACCCTGCGA AGAACACCAC AATTGCCCTA 
CAGGCGGGAT TAAGGACACT GCATGACATT GGGCCAGAAA TCCGGCGTGC TATATCGTGT 
GATTTGCAAG ATGACGAGCC TGAGGAAACA AAACGAGAAG AAGAAGATGA TGTGTTCAAA 
AGAAATGGTG CCCTGCTTGG AAACCATGTC AATCATGTTA ATAGTGATAG GAGAGATTCC 
CTTCAGCAGA CCAATACCAC CCACCGTCCC CTGCATGTCC AAAGGCCTTC AATTCCACCT 
GCAAGTGATA CTGAGAAACC GCTGTTTCCT CCAGCAGGAA ATTCGGTGTG TCATAACCAT 
CATAACCATA ATTCCATAGG AAAGCAAGTT CCCACCTCAA CAAATGCCAA TCTCAATAAT 
GCCAATATGT CCAAAGCTGC CCATGGAAAG CGGCCCAGCA TTGGGAACCT TGAGCATGTG 
TCTGAAAATG GGCATCATTC TTCCCACAAG CATGACCGGG AGCCTCAGAG AAGGTCCAGT 
GTGAAAAGAA CCCGCTATTA TGAAACTTAC ATTAGGTCCG ACTCAGGAGA TGAACAGCTC 
CCAACTATTT GCCGGGAAGA CCCAGAGATA CATGGCTATT TCAGGGACCC CCACTGCTTG 
GGGGAGCAGG AGTATTTCAG TAGTGAGGAA TGCTACGAGG ATGACAGCTC GCCCACCTGG 
AGCAGGCAAA ACTATGGCTA CTACAGCAGA TACCCAGGCA GAAACATCGA CTCTGAGAGG 
CCCCGAGGCT ACCATCATCC CCAAGGATTC TTGGAGGACG ATGACTCGCC CGTTTGCTAT 
GATTCACGGA GATCTCCAAG GAGACGCCTA CTACCTCCCA CCCCAGCATC CCACCGGAGA 
TCCTCCTTCA ACTTTGAGTG CCTGCGCCGG CAGAGCAGCC AGGAAGAGGT CCCGTCGTCT 
CCCATCTTCC CCCATCGCAC GGCCCTGCCT CTGCATCTAA TGCAGCAACA GATCATGGCA 
GTTGCCGGCC TAGATTCAAG TAAAGCCCAG AAGTACTCAC CGAGTCACTC GACCCGGTCG 
TGGGCCACCC CTCCAGCAAC CCCTCCCTAC CGGGACTGGA CACCGTGCTA CACCCCCCTG 
ATCCAAGTGG AGCAGTCAGA GGCCCTGGAC CAGGTGAACG GCAGCCTGCC GTCCCTGCAC 
CGCAGCTCCT GGTACACAGA CGAGCCCGAC ATCTCCTACC GGACTTTCAC ACCAGCCAGC 
CTGACTGTCC CCAGCAGCTT CCGGAACAAA AACAGCGACA AGCAGAGGAG TGCGGACAGC 
TTGGTGGAGG CAGTCCTGAT ATCCGAAGGC TTGGGACGCT ATGCAAGGGA CCCAAAATTT 
GTGTCAGCAA CAAAACACGA AATCGCTGAT GCCTGTGACC TCACCATCGA CGAGATGGAG 
AGTGCAGCCA GCACCCTGCT TAATGGGAAC GTGCGTCCCC GAGCCAACGG GGATGTGGGC 
CCCCTCTCAC ACCGGCAGGA CTATGAGCTA CAGGACTTTG GTCCTGGCTA CAGCGACGAA 
GAGCCAGACC CTGGGAGGGA TGAGGAGGAC CTGGCGGATG AAATGATATG CATCACCACC 
TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_005265448.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005265505.1 Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X12, mRNA.

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CloneID	OHu37463
Clone ID Related Accession (Same CDS sequence)	XM_005265448.3
Accession Version	XM_005265448.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6441bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005265448.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTTCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACTGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTTTT CTGTTTATCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATAGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC AAGCGGCAAA TCTGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTTCGAG TGTTGCGACC ACTTCGACTA GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATCGTAGCT GAAGAGGACC CAGCTCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTAC TGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCG 
AACGGAGGCA TCACCAACTT TGATAACTTT GCCTTTGCCA TGCTTACTGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCA AAGGAAAGAG AGAAGGCAAA AGCACGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCTGAGGA CATCGATCCG GAGAATGAGG AAGAAGGAGG AGAGGAAGGC 
AAACGAAATA CTAGCATGCC CACCAGCGAG ACTGAGTCTG TGAACACAGA GAACGTCAGC 
GGTGAAGGCG AGAACCGAGG CTGCTGTGGA AGTCTCTGTC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGA TTCAATCGCA GAAGATGTAG GGCCGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATC GTCCTGGTGT TTCTGAACAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCAGATTGG TTGACACAGA TTCAAGATAT TGCCAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGCCTCCAA 
GCATATTTCG TCTCTCTTTT CAACCGGTTT GATTGCTTCG TGGTGTGTGG TGGAATCACT 
GAGACGATCT TGGTGGAACT GGAAATCATG TCTCCCCTGG GGATCTCTGT GTTTCGGTGT 
GTGCGCCTCT TAAGAATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCATCCTTAT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATTATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CACTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGACTG GAATGCTGTG ATGTACGATG GCATCATGGC TTACGGGGGC 
CCATCCTCTT CAGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA AGAAGCGGAA GAAAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTAG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA CTATAGAGAA GAGGATGAAG ACAAGGACCC CTATCCGCCT 
TGCGATGTGC CAGTAGGGGA AGAGGAAGAG GAAGAGGAGG AGGATGAACC TGAGGTTCCT 
GCCGGACCCC GTCCTCGAAG GATCTCGGAG TTGAACATGA AGGAAAAAAT TGCCCCCATC 
CCTGAAGGGA GCGCTTTCTT CATTCTTAGC AAGACCAACC CGATCCGCGT AGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC GATACTGGGT 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACTGTTGAGA TCCTGTTGAA GATGACAACT 
TTTGGAGCTT TCCTCCACAA AGGGGCCTTC TGCAGGAACT ACTTCAATTT GCTGGATATG 
CTGGTGGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGT CCCCTCAGGG CCATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG CGTCTTCGTG GCCATCCGGA CCATCGGCAA CATCATGATC 
GTCACCACCC TCCTGCAGTT CATGTTTGCC TGTATCGGGG TCCAGTTGTT CAAGGGGAAG 
TTCTATCGCT GTACGGATGA AGCCAAAAGT AACCCTGAAG AATGCAGGGG ACTTTTCATC 
CTCTACAAGG ATGGGGATGT TGACAGTCCT GTGGTCCGTG AACGGATCTG GCAAAACAGT 
GATTTCAACT TCGACAACGT CCTCTCTGCT ATGATGGCGC TCTTCACAGT CTCCACGTTT 
GAGGGCTGGC CTGCGTTGCT GTATAAAGCC ATCGACTCGA ATGGAGAGAA CATCGGCCCA 
ATCTACAACC ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTAGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTT GTCATCGTTA CATTTCAGGA ACAAGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CTTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACCGGGGT GTTCACCGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCAGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGA TTGGTGAAGC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACAT TGCTGTGGAC TTTTATTAAG TCCTTTCAGG CGCTCCCGTA TGTGGCCCTC 
CTCATAGCCA TGCTGTTCTT CATCTATGCG GTCATTGGCA TGCAGATGTT TGGGAAAGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAATAACT TCCAGACGTT TCCCCAGGCG 
GTGCTGCTGC TCTTCAGGTG TGCAACAGGT GAGGCCTGGC AGGAGATCAT GCTGGCCTGT 
CTCCCAGGGA AGCTCTGTGA CCCTGAGTCA GATTACAACC CCGGGGAGGA GTATACATGT 
GGGAGCAACT TTGCCATTGT CTATTTCATC AGTTTTTACA TGCTCTGTGC ATTTCTGATC 
ATCAATCTGT TTGTGGCTGT CATCATGGAT AATTTCGACT ATCTGACCCG GGACTGGTCT 
ATTTTGGGGC CTCACCATTT AGATGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAG 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTCTGC TTCGACGCAT CCAGCCTCCC 
CTGGGGTTTG GGAAGTTATG TCCACACAGG GTAGCGTGCA AGAGATTAGT TGCCATGAAC 
ATGCCTCTCA ACAGTGACGG GACAGTCATG TTTAATGCAA CCCTGTTTGC TTTGGTTCGA 
ACGGCTCTTA AGATCAAGAC CGAAGGGAAC CTGGAGCAAG CTAATGAAGA ACTTCGGGCT 
GTGATAAAGA AAATTTGGAA GAAAACCAGC ATGAAATTAC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAGTACCC TGCGAAGAAC 
ACCACAATTG CCCTACAGGC GGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CGTGTGATTT GCAAGATGAC GAGCCTGAGG AAACAAAACG AGAAGAAGAA 
GATGATGTGT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ATTCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAATTC CACCTGCAAG TGATACTGAG AAACCGCTGT TTCCTCCAGC AGGAAATTCG 
GTGTGTCATA ACCATCATAA CCATAATTCC ATAGGAAAGC AAGTTCCCAC CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTCCAAA GCTGCCCATG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC ATGTGTCTGA AAATGGGCAT CATTCTTCCC ACAAGCATGA CCGGGAGCCT 
CAGAGAAGGT CCAGTGTGAA AAGAACCCGC TATTATGAAA CTTACATTAG GTCCGACTCA 
GGAGATGAAC AGCTCCCAAC TATTTGCCGG GAAGACCCAG AGATACATGG CTATTTCAGG 
GACCCCCACT GCTTGGGGGA GCAGGAGTAT TTCAGTAGTG AGGAATGCTA CGAGGATGAC 
AGCTCGCCCA CCTGGAGCAG GCAAAACTAT GGCTACTACA GCAGATACCC AGGCAGAAAC 
ATCGACTCTG AGAGGCCCCG AGGCTACCAT CATCCCCAAG GATTCTTGGA GGACGATGAC 
TCGCCCGTTT GCTATGATTC ACGGAGATCT CCAAGGAGAC GCCTACTACC TCCCACCCCA 
GCATCCCACC GGAGATCCTC CTTCAACTTT GAGTGCCTGC GCCGGCAGAG CAGCCAGGAA 
GAGGTCCCGT CGTCTCCCAT CTTCCCCCAT CGCACGGCCC TGCCTCTGCA TCTAATGCAG 
CAACAGATCA TGGCAGTTGC CGGCCTAGAT TCAAGTAAAG CCCAGAAGTA CTCACCGAGT 
CACTCGACCC GGTCGTGGGC CACCCCTCCA GCAACCCCTC CCTACCGGGA CTGGACACCG 
TGCTACACCC CCCTGATCCA AGTGGAGCAG TCAGAGGCCC TGGACCAGGT GAACGGCAGC 
CTGCCGTCCC TGCACCGCAG CTCCTGGTAC ACAGACGAGC CCGACATCTC CTACCGGACT 
TTCACACCAG CCAGCCTGAC TGTCCCCAGC AGCTTCCGGA ACAAAAACAG CGACAAGCAG 
AGGAGTGCGG ACAGCTTGGT GGAGGCAGTC CTGATATCCG AAGGCTTGGG ACGCTATGCA 
AGGGACCCAA AATTTGTGTC AGCAACAAAA CACGAAATCG CTGATGCCTG TGACCTCACC 
ATCGACGAGA TGGAGAGTGC AGCCAGCACC CTGCTTAATG GGAACGTGCG TCCCCGAGCC 
AACGGGGATG TGGGCCCCCT CTCACACCGG CAGGACTATG AGCTACAGGA CTTTGGTCCT 
GGCTACAGCG ACGAAGAGCC AGACCCTGGG AGGGATGAGG AGGACCTGGC GGATGAAATG 
ATATGCATCA CCACCTTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005265505.1
CDS	1..6441
Translation	MMMMMMMKKMQHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGENRGCCGSLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENIGPIYNHRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEDDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGNLEHVSENGHHSSHKHDREPQRRSSVKRTRYYETYIRSDSGDEQLPTICREDPEIHGYFRDPHCLGEQEYFSSEECYEDDSSPTWSRQNYGYYSRYPGRNIDSERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPASHRRSSFNFECLRRQSSQEEVPSSPIFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVEQSEALDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGNVRPRANGDVGPLSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_005265448.3
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005265448.3

ORF Insert Sequence:

ATGATGATGA TGATGATGAT GAAAAAAATG CAGCATCAAC GGCAGCAGCA AGCGGACCAC 
GCGAACGAGG CAAACTATGC AAGAGGCACC AGACTTCCTC TTTCTGGTGA AGGACCAACT 
TCTCAGCCGA ATAGCTCCAA GCAAACTGTC CTGTCTTGGC AAGCTGCAAT CGATGCTGCT 
AGACAGGCCA AGGCTGCCCA AACTATGAGC ACCTCTGCAC CCCCACCTGT AGGATCTCTC 
TCCCAAAGAA AACGTCAGCA ATACGCCAAG AGCAAAAAAC AGGGTAACTC GTCCAACAGC 
CGACCTGCCC GCGCCCTTTT CTGTTTATCA CTCAATAACC CCATCCGAAG AGCCTGCATT 
AGTATAGTGG AATGGAAACC ATTTGACATA TTTATATTAT TGGCTATTTT TGCCAATTGT 
GTGGCCTTAG CTATTTACAT CCCATTCCCT GAAGATGATT CTAATTCAAC AAATCATAAC 
TTGGAAAAAG TAGAATATGC CTTCCTGATT ATTTTTACAG TCGAGACATT TTTGAAGATT 
ATAGCGTATG GATTATTGCT ACATCCTAAT GCTTATGTTA GGAATGGATG GAATTTACTG 
GATTTTGTTA TAGTAATAGT AGGATTGTTT AGTGTAATTT TGGAACAATT AACCAAAGAA 
ACAGAAGGCG GGAACCACTC AAGCGGCAAA TCTGGAGGCT TTGATGTCAA AGCCCTCCGT 
GCCTTTCGAG TGTTGCGACC ACTTCGACTA GTGTCAGGAG TGCCCAGTTT ACAAGTTGTC 
CTGAACTCCA TTATAAAAGC CATGGTTCCC CTCCTTCACA TAGCCCTTTT GGTATTATTT 
GTAATCATAA TCTATGCTAT TATAGGATTG GAACTTTTTA TTGGAAAAAT GCACAAAACA 
TGTTTTTTTG CTGACTCAGA TATCGTAGCT GAAGAGGACC CAGCTCCATG TGCGTTCTCA 
GGGAATGGAC GCCAGTGTAC TGCCAATGGC ACGGAATGTA GGAGTGGCTG GGTTGGCCCG 
AACGGAGGCA TCACCAACTT TGATAACTTT GCCTTTGCCA TGCTTACTGT GTTTCAGTGC 
ATCACCATGG AGGGCTGGAC AGATGTGCTC TACTGGGTAA ATGATGCGAT AGGATGGGAA 
TGGCCATGGG TGTATTTTGT TAGTCTGATC ATCCTTGGCT CATTTTTCGT CCTTAACCTG 
GTTCTTGGTG TCCTTAGTGG AGAATTCTCA AAGGAAAGAG AGAAGGCAAA AGCACGGGGA 
GATTTCCAGA AGCTCCGGGA GAAGCAGCAG CTGGAGGAGG ATCTAAAGGG CTACTTGGAT 
TGGATCACCC AAGCTGAGGA CATCGATCCG GAGAATGAGG AAGAAGGAGG AGAGGAAGGC 
AAACGAAATA CTAGCATGCC CACCAGCGAG ACTGAGTCTG TGAACACAGA GAACGTCAGC 
GGTGAAGGCG AGAACCGAGG CTGCTGTGGA AGTCTCTGTC AAGCCATCTC AAAATCCAAA 
CTCAGCCGAC GCTGGCGTCG CTGGAACCGA TTCAATCGCA GAAGATGTAG GGCCGCCGTG 
AAGTCTGTCA CGTTTTACTG GCTGGTTATC GTCCTGGTGT TTCTGAACAC CTTAACCATT 
TCCTCTGAGC ACTACAATCA GCCAGATTGG TTGACACAGA TTCAAGATAT TGCCAACAAA 
GTCCTCTTGG CTCTGTTCAC CTGCGAGATG CTGGTAAAAA TGTACAGCTT GGGCCTCCAA 
GCATATTTCG TCTCTCTTTT CAACCGGTTT GATTGCTTCG TGGTGTGTGG TGGAATCACT 
GAGACGATCT TGGTGGAACT GGAAATCATG TCTCCCCTGG GGATCTCTGT GTTTCGGTGT 
GTGCGCCTCT TAAGAATCTT CAAAGTGACC AGGCACTGGA CTTCCCTGAG CAACTTAGTG 
GCATCCTTAT TAAACTCCAT GAAGTCCATC GCTTCGCTGT TGCTTCTGCT TTTTCTCTTC 
ATTATCATCT TTTCCTTGCT TGGGATGCAG CTGTTTGGCG GCAAGTTTAA TTTTGATGAA 
ACGCAAACCA AGCGGAGCAC CTTTGACAAT TTCCCTCAAG CACTTCTCAC AGTGTTCCAG 
ATCCTGACAG GCGAAGACTG GAATGCTGTG ATGTACGATG GCATCATGGC TTACGGGGGC 
CCATCCTCTT CAGGAATGAT CGTCTGCATC TACTTCATCA TCCTCTTCAT TTGTGGTAAC 
TATATTCTAC TGAATGTCTT CTTGGCCATC GCTGTAGACA ATTTGGCTGA TGCTGAAAGT 
CTGAACACTG CTCAGAAAGA AGAAGCGGAA GAAAAGGAGA GGAAAAAGAT TGCCAGAAAA 
GAGAGCCTAG AAAATAAAAA GAACAACAAA CCAGAAGTCA ACCAGATAGC CAACAGTGAC 
AACAAGGTTA CAATTGATGA CTATAGAGAA GAGGATGAAG ACAAGGACCC CTATCCGCCT 
TGCGATGTGC CAGTAGGGGA AGAGGAAGAG GAAGAGGAGG AGGATGAACC TGAGGTTCCT 
GCCGGACCCC GTCCTCGAAG GATCTCGGAG TTGAACATGA AGGAAAAAAT TGCCCCCATC 
CCTGAAGGGA GCGCTTTCTT CATTCTTAGC AAGACCAACC CGATCCGCGT AGGCTGCCAC 
AAGCTCATCA ACCACCACAT CTTCACCAAC CTCATCCTTG TCTTCATCAT GCTGAGCAGC 
GCTGCCCTGG CCGCAGAGGA CCCCATCCGC AGCCACTCCT TCCGGAACAC GATACTGGGT 
TACTTTGACT ATGCCTTCAC AGCCATCTTT ACTGTTGAGA TCCTGTTGAA GATGACAACT 
TTTGGAGCTT TCCTCCACAA AGGGGCCTTC TGCAGGAACT ACTTCAATTT GCTGGATATG 
CTGGTGGTTG GGGTGTCTCT GGTGTCATTT GGGATTCAAT CCAGTGCCAT CTCCGTTGTG 
AAGATTCTGA GGGTCTTAAG GGTCCTGCGT CCCCTCAGGG CCATCAACAG AGCAAAAGGA 
CTTAAGCACG TGGTCCAGTG CGTCTTCGTG GCCATCCGGA CCATCGGCAA CATCATGATC 
GTCACCACCC TCCTGCAGTT CATGTTTGCC TGTATCGGGG TCCAGTTGTT CAAGGGGAAG 
TTCTATCGCT GTACGGATGA AGCCAAAAGT AACCCTGAAG AATGCAGGGG ACTTTTCATC 
CTCTACAAGG ATGGGGATGT TGACAGTCCT GTGGTCCGTG AACGGATCTG GCAAAACAGT 
GATTTCAACT TCGACAACGT CCTCTCTGCT ATGATGGCGC TCTTCACAGT CTCCACGTTT 
GAGGGCTGGC CTGCGTTGCT GTATAAAGCC ATCGACTCGA ATGGAGAGAA CATCGGCCCA 
ATCTACAACC ACCGCGTGGA GATCTCCATC TTCTTCATCA TCTACATCAT CATTGTAGCT 
TTCTTCATGA TGAACATCTT TGTGGGCTTT GTCATCGTTA CATTTCAGGA ACAAGGAGAA 
AAAGAGTATA AGAACTGTGA GCTGGACAAA AATCAGCGTC AGTGTGTTGA ATACGCCTTG 
AAAGCACGTC CCTTGCGGAG ATACATCCCC AAAAACCCCT ACCAGTACAA GTTCTGGTAC 
GTGGTGAACT CTTCGCCTTT CGAATACATG ATGTTTGTCC TCATCATGCT CAACACACTC 
TGCTTGGCCA TGCAGCACTA CGAGCAGTCC AAGATGTTCA ATGATGCCAT GGACATTCTG 
AACATGGTCT TCACCGGGGT GTTCACCGTC GAGATGGTTT TGAAAGTCAT CGCATTTAAG 
CCTAAGGGGT ATTTTAGTGA CGCCTGGAAC ACGTTTGACT CCCTCATCGT AATCGGCAGC 
ATTATAGACG TGGCCCTCAG CGAAGCAGAC AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGA TTGGTGAAGC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACAT TGCTGTGGAC TTTTATTAAG TCCTTTCAGG CGCTCCCGTA TGTGGCCCTC 
CTCATAGCCA TGCTGTTCTT CATCTATGCG GTCATTGGCA TGCAGATGTT TGGGAAAGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAATAACT TCCAGACGTT TCCCCAGGCG 
GTGCTGCTGC TCTTCAGGTG TGCAACAGGT GAGGCCTGGC AGGAGATCAT GCTGGCCTGT 
CTCCCAGGGA AGCTCTGTGA CCCTGAGTCA GATTACAACC CCGGGGAGGA GTATACATGT 
GGGAGCAACT TTGCCATTGT CTATTTCATC AGTTTTTACA TGCTCTGTGC ATTTCTGATC 
ATCAATCTGT TTGTGGCTGT CATCATGGAT AATTTCGACT ATCTGACCCG GGACTGGTCT 
ATTTTGGGGC CTCACCATTT AGATGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAG 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTCTGC TTCGACGCAT CCAGCCTCCC 
CTGGGGTTTG GGAAGTTATG TCCACACAGG GTAGCGTGCA AGAGATTAGT TGCCATGAAC 
ATGCCTCTCA ACAGTGACGG GACAGTCATG TTTAATGCAA CCCTGTTTGC TTTGGTTCGA 
ACGGCTCTTA AGATCAAGAC CGAAGGGAAC CTGGAGCAAG CTAATGAAGA ACTTCGGGCT 
GTGATAAAGA AAATTTGGAA GAAAACCAGC ATGAAATTAC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAGTACCC TGCGAAGAAC 
ACCACAATTG CCCTACAGGC GGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CGTGTGATTT GCAAGATGAC GAGCCTGAGG AAACAAAACG AGAAGAAGAA 
GATGATGTGT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ATTCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAATTC CACCTGCAAG TGATACTGAG AAACCGCTGT TTCCTCCAGC AGGAAATTCG 
GTGTGTCATA ACCATCATAA CCATAATTCC ATAGGAAAGC AAGTTCCCAC CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTCCAAA GCTGCCCATG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC ATGTGTCTGA AAATGGGCAT CATTCTTCCC ACAAGCATGA CCGGGAGCCT 
CAGAGAAGGT CCAGTGTGAA AAGAACCCGC TATTATGAAA CTTACATTAG GTCCGACTCA 
GGAGATGAAC AGCTCCCAAC TATTTGCCGG GAAGACCCAG AGATACATGG CTATTTCAGG 
GACCCCCACT GCTTGGGGGA GCAGGAGTAT TTCAGTAGTG AGGAATGCTA CGAGGATGAC 
AGCTCGCCCA CCTGGAGCAG GCAAAACTAT GGCTACTACA GCAGATACCC AGGCAGAAAC 
ATCGACTCTG AGAGGCCCCG AGGCTACCAT CATCCCCAAG GATTCTTGGA GGACGATGAC 
TCGCCCGTTT GCTATGATTC ACGGAGATCT CCAAGGAGAC GCCTACTACC TCCCACCCCA 
GCATCCCACC GGAGATCCTC CTTCAACTTT GAGTGCCTGC GCCGGCAGAG CAGCCAGGAA 
GAGGTCCCGT CGTCTCCCAT CTTCCCCCAT CGCACGGCCC TGCCTCTGCA TCTAATGCAG 
CAACAGATCA TGGCAGTTGC CGGCCTAGAT TCAAGTAAAG CCCAGAAGTA CTCACCGAGT 
CACTCGACCC GGTCGTGGGC CACCCCTCCA GCAACCCCTC CCTACCGGGA CTGGACACCG 
TGCTACACCC CCCTGATCCA AGTGGAGCAG TCAGAGGCCC TGGACCAGGT GAACGGCAGC 
CTGCCGTCCC TGCACCGCAG CTCCTGGTAC ACAGACGAGC CCGACATCTC CTACCGGACT 
TTCACACCAG CCAGCCTGAC TGTCCCCAGC AGCTTCCGGA ACAAAAACAG CGACAAGCAG 
AGGAGTGCGG ACAGCTTGGT GGAGGCAGTC CTGATATCCG AAGGCTTGGG ACGCTATGCA 
AGGGACCCAA AATTTGTGTC AGCAACAAAA CACGAAATCG CTGATGCCTG TGACCTCACC 
ATCGACGAGA TGGAGAGTGC AGCCAGCACC CTGCTTAATG GGAACGTGCG TCCCCGAGCC 
AACGGGGATG TGGGCCCCCT CTCACACCGG CAGGACTATG AGCTACAGGA CTTTGGTCCT 
GGCTACAGCG ACGAAGAGCC AGACCCTGGG AGGGATGAGG AGGACCTGGC GGATGAAATG 
ATATGCATCA CCACCTTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_011534094.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011532396.1 Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X2, mRNA.

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CloneID	OHu56576
Clone ID Related Accession (Same CDS sequence)	XM_011534094.2
Accession Version	XM_011534094.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011534094.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGGG AGCCGCTGCC GCTGTGGGGC TCCCCTCCCC AGCTCTGGCC CGGGCACCAC 
GTGCAGCTTC CACGCCGGCC GGGGCTGGGT CGAGGGAGGA TGGAGCAGCT CCTGGCCAGC 
CCTCCCCTTT GGAGACCGCC AGTGCCCCCC AACCCCTGTC GGCTGGAAGC CTTTCCGGAG 
GCAAACTATG CAAGAGGCAC CAGACTTCCT CTTTCTGGTG AAGGACCAAC TTCTCAGCCG 
AATAGCTCCA AGCAAACTGT CCTGTCTTGG CAAGCTGCAA TCGATGCTGC TAGACAGGCC 
AAGGCTGCCC AAACTATGAG CACCTCTGCA CCCCCACCTG TAGGATCTCT CTCCCAAAGA 
AAACGTCAGC AATACGCCAA GAGCAAAAAA CAGGGTAACT CGTCCAACAG CCGACCTGCC 
CGCGCCCTTT TCTGTTTATC ACTCAATAAC CCCATCCGAA GAGCCTGCAT TAGTATAGTG 
GAATGGAAAC CATTTGACAT ATTTATATTA TTGGCTATTT TTGCCAATTG TGTGGCCTTA 
GCTATTTACA TCCCATTCCC TGAAGATGAT TCTAATTCAA CAAATCATAA CTTGGAAAAA 
GTAGAATATG CCTTCCTGAT TATTTTTACA GTCGAGACAT TTTTGAAGAT TATAGCGTAT 
GGATTATTGC TACATCCTAA TGCTTATGTT AGGAATGGAT GGAATTTACT GGATTTTGTT 
ATAGTAATAG TAGGATTGTT TAGTGTAATT TTGGAACAAT TAACCAAAGA AACAGAAGGC 
GGGAACCACT CAAGCGGCAA ATCTGGAGGC TTTGATGTCA AAGCCCTCCG TGCCTTTCGA 
GTGTTGCGAC CACTTCGACT AGTGTCAGGA GTGCCCAGTT TACAAGTTGT CCTGAACTCC 
ATTATAAAAG CCATGGTTCC CCTCCTTCAC ATAGCCCTTT TGGTATTATT TGTAATCATA 
ATCTATGCTA TTATAGGATT GGAACTTTTT ATTGGAAAAA TGCACAAAAC ATGTTTTTTT 
GCTGACTCAG ATATCGTAGC TGAAGAGGAC CCAGCTCCAT GTGCGTTCTC AGGGAATGGA 
CGCCAGTGTA CTGCCAATGG CACGGAATGT AGGAGTGGCT GGGTTGGCCC GAACGGAGGC 
ATCACCAACT TTGATAACTT TGCCTTTGCC ATGCTTACTG TGTTTCAGTG CATCACCATG 
GAGGGCTGGA CAGATGTGCT CTACTGGATG AATGATGCTA TGGGATTTGA ATTGCCCTGG 
GTGTATTTTG TCAGTCTCGT CATCTTTGGG TCATTTTTCG TACTAAATCT TGTACTTGGT 
GTATTGAGCG GAGAATTCTC AAAGGAAAGA GAGAAGGCAA AAGCACGGGG AGATTTCCAG 
AAGCTCCGGG AGAAGCAGCA GCTGGAGGAG GATCTAAAGG GCTACTTGGA TTGGATCACC 
CAAGCTGAGG ACATCGATCC GGAGAATGAG GAAGAAGGAG GAGAGGAAGG CAAACGAAAT 
ACTAGCATGC CCACCAGCGA GACTGAGTCT GTGAACACAG AGAACGTCAG CGGTGAAGGC 
GAGAACCGAG GCTGCTGTGG AAGTCTCTGG TGCTGGTGGA GACGGAGAGG CGCGGCCAAG 
GCGGGGCCCT CTGGGTGTCG GCGGTGGGGT CAAGCCATCT CAAAATCCAA ACTCAGCCGA 
CGCTGGCGTC GCTGGAACCG ATTCAATCGC AGAAGATGTA GGGCCGCCGT GAAGTCTGTC 
ACGTTTTACT GGCTGGTTAT CGTCCTGGTG TTTCTGAACA CCTTAACCAT TTCCTCTGAG 
CACTACAATC AGCCAGATTG GTTGACACAG ATTCAAGATA TTGCCAACAA AGTCCTCTTG 
GCTCTGTTCA CCTGCGAGAT GCTGGTAAAA ATGTACAGCT TGGGCCTCCA AGCATATTTC 
GTCTCTCTTT TCAACCGGTT TGATTGCTTC GTGGTGTGTG GTGGAATCAC TGAGACGATC 
TTGGTGGAAC TGGAAATCAT GTCTCCCCTG GGGATCTCTG TGTTTCGGTG TGTGCGCCTC 
TTAAGAATCT TCAAAGTGAC CAGGCACTGG ACTTCCCTGA GCAACTTAGT GGCATCCTTA 
TTAAACTCCA TGAAGTCCAT CGCTTCGCTG TTGCTTCTGC TTTTTCTCTT CATTATCATC 
TTTTCCTTGC TTGGGATGCA GCTGTTTGGC GGCAAGTTTA ATTTTGATGA AACGCAAACC 
AAGCGGAGCA CCTTTGACAA TTTCCCTCAA GCACTTCTCA CAGTGTTCCA GATCCTGACA 
GGCGAAGACT GGAATGCTGT GATGTACGAT GGCATCATGG CTTACGGGGG CCCATCCTCT 
TCAGGAATGA TCGTCTGCAT CTACTTCATC ATCCTCTTCA TTTGTGGTAA CTATATTCTA 
CTGAATGTCT TCTTGGCCAT CGCTGTAGAC AATTTGGCTG ATGCTGAAAG TCTGAACACT 
GCTCAGAAAG AAGAAGCGGA AGAAAAGGAG AGGAAAAAGA TTGCCAGAAA AGAGAGCCTA 
GAAAATAAAA AGAACAACAA ACCAGAAGTC AACCAGATAG CCAACAGTGA CAACAAGGTT 
ACAATTGATG ACTATAGAGA AGAGGATGAA GACAAGGACC CCTATCCGCC TTGCGATGTG 
CCAGTAGGGG AAGAGGAAGA GGAAGAGGAG GAGGATGAAC CTGAGGTTCC TGCCGGACCC 
CGTCCTCGAA GGATCTCGGA GTTGAACATG AAGGAAAAAA TTGCCCCCAT CCCTGAAGGG 
AGCGCTTTCT TCATTCTTAG CAAGACCAAC CCGATCCGCG TAGGCTGCCA CAAGCTCATC 
AACCACCACA TCTTCACCAA CCTCATCCTT GTCTTCATCA TGCTGAGCAG CGCTGCCCTG 
GCCGCAGAGG ACCCCATCCG CAGCCACTCC TTCCGGAACA CGATACTGGG TTACTTTGAC 
TATGCCTTCA CAGCCATCTT TACTGTTGAG ATCCTGTTGA AGATGACAAC TTTTGGAGCT 
TTCCTCCACA AAGGGGCCTT CTGCAGGAAC TACTTCAATT TGCTGGATAT GCTGGTGGTT 
GGGGTGTCTC TGGTGTCATT TGGGATTCAA TCCAGTGCCA TCTCCGTTGT GAAGATTCTG 
AGGGTCTTAA GGGTCCTGCG TCCCCTCAGG GCCATCAACA GAGCAAAAGG ACTTAAGCAC 
GTGGTCCAGT GCGTCTTCGT GGCCATCCGG ACCATCGGCA ACATCATGAT CGTCACCACC 
CTCCTGCAGT TCATGTTTGC CTGTATCGGG GTCCAGTTGT TCAAGGGGAA GTTCTATCGC 
TGTACGGATG AAGCCAAAAG TAACCCTGAA GAATGCAGGG GACTTTTCAT CCTCTACAAG 
GATGGGGATG TTGACAGTCC TGTGGTCCGT GAACGGATCT GGCAAAACAG TGATTTCAAC 
TTCGACAACG TCCTCTCTGC TATGATGGCG CTCTTCACAG TCTCCACGTT TGAGGGCTGG 
CCTGCGTTGC TGTATAAAGC CATCGACTCG AATGGAGAGA ACATCGGCCC AATCTACAAC 
CACCGCGTGG AGATCTCCAT CTTCTTCATC ATCTACATCA TCATTGTAGC TTTCTTCATG 
ATGAACATCT TTGTGGGCTT TGTCATCGTT ACATTTCAGG AACAAGGAGA AAAAGAGTAT 
AAGAACTGTG AGCTGGACAA AAATCAGCGT CAGTGTGTTG AATACGCCTT GAAAGCACGT 
CCCTTGCGGA GATACATCCC CAAAAACCCC TACCAGTACA AGTTCTGGTA CGTGGTGAAC 
TCTTCGCCTT TCGAATACAT GATGTTTGTC CTCATCATGC TCAACACACT CTGCTTGGCC 
ATGCAGCACT ACGAGCAGTC CAAGATGTTC AATGATGCCA TGGACATTCT GAACATGGTC 
TTCACCGGGG TGTTCACCGT CGAGATGGTT TTGAAAGTCA TCGCATTTAA GCCTAAGGGG 
TATTTTAGTG ACGCCTGGAA CACGTTTGAC TCCCTCATCG TAATCGGCAG CATTATAGAC 
GTGGCCCTCA GCGAAGCAGA CCACTATTTC ACTGATGCAT GGAACACTTT TGATGCCTTA 
ATTGTTGTTG GTAGCGTCGT TGATATTGCT ATAACTGAAG TGAATCCAAC TGAAAGTGAA 
AATGTCCCTG TCCCAACTGC TACACCTGGG AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGA TTGGTGAAGC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACAT TGCTGTGGAC TTTTATTAAG TCCTTTCAGG CGCTCCCGTA TGTGGCCCTC 
CTCATAGCCA TGCTGTTCTT CATCTATGCG GTCATTGGCA TGCAGATGTT TGGGAAAGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAATAACT TCCAGACGTT TCCCCAGGCG 
GTGCTGCTGC TCTTCAGGTG TGCAACAGGT GAGGCCTGGC AGGAGATCAT GCTGGCCTGT 
CTCCCAGGGA AGCTCTGTGA CCCTGAGTCA GATTACAACC CCGGGGAGGA GTATACATGT 
GGGAGCAACT TTGCCATTGT CTATTTCATC AGTTTTTACA TGCTCTGTGC ATTTCTGATC 
ATCAATCTGT TTGTGGCTGT CATCATGGAT AATTTCGACT ATCTGACCCG GGACTGGTCT 
ATTTTGGGGC CTCACCATTT AGATGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAG 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTCTGC TTCGACGCAT CCAGCCTCCC 
CTGGGGTTTG GGAAGTTATG TCCACACAGG GTAGCGTGCA AGAGATTAGT TGCCATGAAC 
ATGCCTCTCA ACAGTGACGG GACAGTCATG TTTAATGCAA CCCTGTTTGC TTTGGTTCGA 
ACGGCTCTTA AGATCAAGAC CGAAGGGAAC CTGGAGCAAG CTAATGAAGA ACTTCGGGCT 
GTGATAAAGA AAATTTGGAA GAAAACCAGC ATGAAATTAC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAGTACCC TGCGAAGAAC 
ACCACAATTG CCCTACAGGC GGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CGTGTGATTT GCAAGATGAC GAGCCTGAGG AAACAAAACG AGAAGAAGAA 
GATGATGTGT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ATTCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAATTC CACCTGCAAG TGATACTGAG AAACCGCTGT TTCCTCCAGC AGGAAATTCG 
GTGTGTCATA ACCATCATAA CCATAATTCC ATAGGAAAGC AAGTTCCCAC CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTCCAAA GCTGCCCATG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC ATGTGTCTGA AAATGGGCAT CATTCTTCCC ACAAGCATGA CCGGGAGCCT 
CAGAGAAGGT CCAGTGTGAA AAGAACCCGC TATTATGAAA CTTACATTAG GTCCGACTCA 
GGAGATGAAC AGCTCCCAAC TATTTGCCGG GAAGACCCAG AGATACATGG CTATTTCAGG 
GACCCCCACT GCTTGGGGGA GCAGGAGTAT TTCAGTAGTG AGGAATGCTA CGAGGATGAC 
AGCTCGCCCA CCTGGAGCAG GCAAAACTAT GGCTACTACA GCAGATACCC AGGCAGAAAC 
ATCGACTCTG AGAGGCCCCG AGGCTACCAT CATCCCCAAG GATTCTTGGA GGACGATGAC 
TCGCCCGTTT GCTATGATTC ACGGAGATCT CCAAGGAGAC GCCTACTACC TCCCACCCCA 
GCATCCCACC GGAGATCCTC CTTCAACTTT GAGTGCCTGC GCCGGCAGAG CAGCCAGGAA 
GAGGTCCCGT CGTCTCCCAT CTTCCCCCAT CGCACGGCCC TGCCTCTGCA TCTAATGCAG 
CAACAGATCA TGGCAGTTGC CGGCCTAGAT TCAAGTAAAG CCCAGAAGTA CTCACCGAGT 
CACTCGACCC GGTCGTGGGC CACCCCTCCA GCAACCCCTC CCTACCGGGA CTGGACACCG 
TGCTACACCC CCCTGATCCA AGTGGAGCAG TCAGAGGCCC TGGACCAGGT GAACGGCAGC 
CTGCCGTCCC TGCACCGCAG CTCCTGGTAC ACAGACGAGC CCGACATCTC CTACCGGACT 
TTCACACCAG CCAGCCTGAC TGTCCCCAGC AGCTTCCGGA ACAAAAACAG CGACAAGCAG 
AGGAGTGCGG ACAGCTTGGT GGAGGCAGTC CTGATATCCG AAGGCTTGGG ACGCTATGCA 
AGGGACCCAA AATTTGTGTC AGCAACAAAA CACGAAATCG CTGATGCCTG TGACCTCACC 
ATCGACGAGA TGGAGAGTGC AGCCAGCACC CTGCTTAATG GGAACGTGCG TCCCCGAGCC 
AACGGGGATG TGGGCCCCCT CTCACACCGG CAGGACTATG AGCTACAGGA CTTTGGTCCT 
GGCTACAGCG ACGAAGAGCC AGACCCTGGG AGGGATGAGG AGGACCTGGC GGATGAAATG 
ATATGCATCA CCACCTTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011532396.1
CDS	16..6756
Translation	MPGEPLPLWGSPPQLWPGHHVQLPRRPGLGRGRMEQLLASPPLWRPPVPPNPCRLEAFPEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWMNDAMGFELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGENRGCCGSLWCWWRRRGAAKAGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENIGPIYNHRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPTESENVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEDDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGNLEHVSENGHHSSHKHDREPQRRSSVKRTRYYETYIRSDSGDEQLPTICREDPEIHGYFRDPHCLGEQEYFSSEECYEDDSSPTWSRQNYGYYSRYPGRNIDSERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPASHRRSSFNFECLRRQSSQEEVPSSPIFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVEQSEALDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGNVRPRANGDVGPLSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_011534094.2
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011534094.2

ORF Insert Sequence:

ATGCCCGGGG AGCCGCTGCC GCTGTGGGGC TCCCCTCCCC AGCTCTGGCC CGGGCACCAC 
GTGCAGCTTC CACGCCGGCC GGGGCTGGGT CGAGGGAGGA TGGAGCAGCT CCTGGCCAGC 
CCTCCCCTTT GGAGACCGCC AGTGCCCCCC AACCCCTGTC GGCTGGAAGC CTTTCCGGAG 
GCAAACTATG CAAGAGGCAC CAGACTTCCT CTTTCTGGTG AAGGACCAAC TTCTCAGCCG 
AATAGCTCCA AGCAAACTGT CCTGTCTTGG CAAGCTGCAA TCGATGCTGC TAGACAGGCC 
AAGGCTGCCC AAACTATGAG CACCTCTGCA CCCCCACCTG TAGGATCTCT CTCCCAAAGA 
AAACGTCAGC AATACGCCAA GAGCAAAAAA CAGGGTAACT CGTCCAACAG CCGACCTGCC 
CGCGCCCTTT TCTGTTTATC ACTCAATAAC CCCATCCGAA GAGCCTGCAT TAGTATAGTG 
GAATGGAAAC CATTTGACAT ATTTATATTA TTGGCTATTT TTGCCAATTG TGTGGCCTTA 
GCTATTTACA TCCCATTCCC TGAAGATGAT TCTAATTCAA CAAATCATAA CTTGGAAAAA 
GTAGAATATG CCTTCCTGAT TATTTTTACA GTCGAGACAT TTTTGAAGAT TATAGCGTAT 
GGATTATTGC TACATCCTAA TGCTTATGTT AGGAATGGAT GGAATTTACT GGATTTTGTT 
ATAGTAATAG TAGGATTGTT TAGTGTAATT TTGGAACAAT TAACCAAAGA AACAGAAGGC 
GGGAACCACT CAAGCGGCAA ATCTGGAGGC TTTGATGTCA AAGCCCTCCG TGCCTTTCGA 
GTGTTGCGAC CACTTCGACT AGTGTCAGGA GTGCCCAGTT TACAAGTTGT CCTGAACTCC 
ATTATAAAAG CCATGGTTCC CCTCCTTCAC ATAGCCCTTT TGGTATTATT TGTAATCATA 
ATCTATGCTA TTATAGGATT GGAACTTTTT ATTGGAAAAA TGCACAAAAC ATGTTTTTTT 
GCTGACTCAG ATATCGTAGC TGAAGAGGAC CCAGCTCCAT GTGCGTTCTC AGGGAATGGA 
CGCCAGTGTA CTGCCAATGG CACGGAATGT AGGAGTGGCT GGGTTGGCCC GAACGGAGGC 
ATCACCAACT TTGATAACTT TGCCTTTGCC ATGCTTACTG TGTTTCAGTG CATCACCATG 
GAGGGCTGGA CAGATGTGCT CTACTGGATG AATGATGCTA TGGGATTTGA ATTGCCCTGG 
GTGTATTTTG TCAGTCTCGT CATCTTTGGG TCATTTTTCG TACTAAATCT TGTACTTGGT 
GTATTGAGCG GAGAATTCTC AAAGGAAAGA GAGAAGGCAA AAGCACGGGG AGATTTCCAG 
AAGCTCCGGG AGAAGCAGCA GCTGGAGGAG GATCTAAAGG GCTACTTGGA TTGGATCACC 
CAAGCTGAGG ACATCGATCC GGAGAATGAG GAAGAAGGAG GAGAGGAAGG CAAACGAAAT 
ACTAGCATGC CCACCAGCGA GACTGAGTCT GTGAACACAG AGAACGTCAG CGGTGAAGGC 
GAGAACCGAG GCTGCTGTGG AAGTCTCTGG TGCTGGTGGA GACGGAGAGG CGCGGCCAAG 
GCGGGGCCCT CTGGGTGTCG GCGGTGGGGT CAAGCCATCT CAAAATCCAA ACTCAGCCGA 
CGCTGGCGTC GCTGGAACCG ATTCAATCGC AGAAGATGTA GGGCCGCCGT GAAGTCTGTC 
ACGTTTTACT GGCTGGTTAT CGTCCTGGTG TTTCTGAACA CCTTAACCAT TTCCTCTGAG 
CACTACAATC AGCCAGATTG GTTGACACAG ATTCAAGATA TTGCCAACAA AGTCCTCTTG 
GCTCTGTTCA CCTGCGAGAT GCTGGTAAAA ATGTACAGCT TGGGCCTCCA AGCATATTTC 
GTCTCTCTTT TCAACCGGTT TGATTGCTTC GTGGTGTGTG GTGGAATCAC TGAGACGATC 
TTGGTGGAAC TGGAAATCAT GTCTCCCCTG GGGATCTCTG TGTTTCGGTG TGTGCGCCTC 
TTAAGAATCT TCAAAGTGAC CAGGCACTGG ACTTCCCTGA GCAACTTAGT GGCATCCTTA 
TTAAACTCCA TGAAGTCCAT CGCTTCGCTG TTGCTTCTGC TTTTTCTCTT CATTATCATC 
TTTTCCTTGC TTGGGATGCA GCTGTTTGGC GGCAAGTTTA ATTTTGATGA AACGCAAACC 
AAGCGGAGCA CCTTTGACAA TTTCCCTCAA GCACTTCTCA CAGTGTTCCA GATCCTGACA 
GGCGAAGACT GGAATGCTGT GATGTACGAT GGCATCATGG CTTACGGGGG CCCATCCTCT 
TCAGGAATGA TCGTCTGCAT CTACTTCATC ATCCTCTTCA TTTGTGGTAA CTATATTCTA 
CTGAATGTCT TCTTGGCCAT CGCTGTAGAC AATTTGGCTG ATGCTGAAAG TCTGAACACT 
GCTCAGAAAG AAGAAGCGGA AGAAAAGGAG AGGAAAAAGA TTGCCAGAAA AGAGAGCCTA 
GAAAATAAAA AGAACAACAA ACCAGAAGTC AACCAGATAG CCAACAGTGA CAACAAGGTT 
ACAATTGATG ACTATAGAGA AGAGGATGAA GACAAGGACC CCTATCCGCC TTGCGATGTG 
CCAGTAGGGG AAGAGGAAGA GGAAGAGGAG GAGGATGAAC CTGAGGTTCC TGCCGGACCC 
CGTCCTCGAA GGATCTCGGA GTTGAACATG AAGGAAAAAA TTGCCCCCAT CCCTGAAGGG 
AGCGCTTTCT TCATTCTTAG CAAGACCAAC CCGATCCGCG TAGGCTGCCA CAAGCTCATC 
AACCACCACA TCTTCACCAA CCTCATCCTT GTCTTCATCA TGCTGAGCAG CGCTGCCCTG 
GCCGCAGAGG ACCCCATCCG CAGCCACTCC TTCCGGAACA CGATACTGGG TTACTTTGAC 
TATGCCTTCA CAGCCATCTT TACTGTTGAG ATCCTGTTGA AGATGACAAC TTTTGGAGCT 
TTCCTCCACA AAGGGGCCTT CTGCAGGAAC TACTTCAATT TGCTGGATAT GCTGGTGGTT 
GGGGTGTCTC TGGTGTCATT TGGGATTCAA TCCAGTGCCA TCTCCGTTGT GAAGATTCTG 
AGGGTCTTAA GGGTCCTGCG TCCCCTCAGG GCCATCAACA GAGCAAAAGG ACTTAAGCAC 
GTGGTCCAGT GCGTCTTCGT GGCCATCCGG ACCATCGGCA ACATCATGAT CGTCACCACC 
CTCCTGCAGT TCATGTTTGC CTGTATCGGG GTCCAGTTGT TCAAGGGGAA GTTCTATCGC 
TGTACGGATG AAGCCAAAAG TAACCCTGAA GAATGCAGGG GACTTTTCAT CCTCTACAAG 
GATGGGGATG TTGACAGTCC TGTGGTCCGT GAACGGATCT GGCAAAACAG TGATTTCAAC 
TTCGACAACG TCCTCTCTGC TATGATGGCG CTCTTCACAG TCTCCACGTT TGAGGGCTGG 
CCTGCGTTGC TGTATAAAGC CATCGACTCG AATGGAGAGA ACATCGGCCC AATCTACAAC 
CACCGCGTGG AGATCTCCAT CTTCTTCATC ATCTACATCA TCATTGTAGC TTTCTTCATG 
ATGAACATCT TTGTGGGCTT TGTCATCGTT ACATTTCAGG AACAAGGAGA AAAAGAGTAT 
AAGAACTGTG AGCTGGACAA AAATCAGCGT CAGTGTGTTG AATACGCCTT GAAAGCACGT 
CCCTTGCGGA GATACATCCC CAAAAACCCC TACCAGTACA AGTTCTGGTA CGTGGTGAAC 
TCTTCGCCTT TCGAATACAT GATGTTTGTC CTCATCATGC TCAACACACT CTGCTTGGCC 
ATGCAGCACT ACGAGCAGTC CAAGATGTTC AATGATGCCA TGGACATTCT GAACATGGTC 
TTCACCGGGG TGTTCACCGT CGAGATGGTT TTGAAAGTCA TCGCATTTAA GCCTAAGGGG 
TATTTTAGTG ACGCCTGGAA CACGTTTGAC TCCCTCATCG TAATCGGCAG CATTATAGAC 
GTGGCCCTCA GCGAAGCAGA CCACTATTTC ACTGATGCAT GGAACACTTT TGATGCCTTA 
ATTGTTGTTG GTAGCGTCGT TGATATTGCT ATAACTGAAG TGAATCCAAC TGAAAGTGAA 
AATGTCCCTG TCCCAACTGC TACACCTGGG AACTCTGAAG AGAGCAATAG AATCTCCATC 
ACCTTTTTCC GTCTTTTCCG AGTGATGCGA TTGGTGAAGC TTCTCAGCAG GGGGGAAGGC 
ATCCGGACAT TGCTGTGGAC TTTTATTAAG TCCTTTCAGG CGCTCCCGTA TGTGGCCCTC 
CTCATAGCCA TGCTGTTCTT CATCTATGCG GTCATTGGCA TGCAGATGTT TGGGAAAGTT 
GCCATGAGAG ATAACAACCA GATCAATAGG AACAATAACT TCCAGACGTT TCCCCAGGCG 
GTGCTGCTGC TCTTCAGGTG TGCAACAGGT GAGGCCTGGC AGGAGATCAT GCTGGCCTGT 
CTCCCAGGGA AGCTCTGTGA CCCTGAGTCA GATTACAACC CCGGGGAGGA GTATACATGT 
GGGAGCAACT TTGCCATTGT CTATTTCATC AGTTTTTACA TGCTCTGTGC ATTTCTGATC 
ATCAATCTGT TTGTGGCTGT CATCATGGAT AATTTCGACT ATCTGACCCG GGACTGGTCT 
ATTTTGGGGC CTCACCATTT AGATGAATTC AAAAGAATAT GGTCAGAATA TGACCCTGAG 
GCAAAGGGAA GGATAAAACA CCTTGATGTG GTCACTCTGC TTCGACGCAT CCAGCCTCCC 
CTGGGGTTTG GGAAGTTATG TCCACACAGG GTAGCGTGCA AGAGATTAGT TGCCATGAAC 
ATGCCTCTCA ACAGTGACGG GACAGTCATG TTTAATGCAA CCCTGTTTGC TTTGGTTCGA 
ACGGCTCTTA AGATCAAGAC CGAAGGGAAC CTGGAGCAAG CTAATGAAGA ACTTCGGGCT 
GTGATAAAGA AAATTTGGAA GAAAACCAGC ATGAAATTAC TTGACCAAGT TGTCCCTCCA 
GCTGGTGATG ATGAGGTAAC CGTGGGGAAG TTCTATGCCA CTTTCCTGAT ACAGGACTAC 
TTTAGGAAAT TCAAGAAACG GAAAGAACAA GGACTGGTGG GAAAGTACCC TGCGAAGAAC 
ACCACAATTG CCCTACAGGC GGGATTAAGG ACACTGCATG ACATTGGGCC AGAAATCCGG 
CGTGCTATAT CGTGTGATTT GCAAGATGAC GAGCCTGAGG AAACAAAACG AGAAGAAGAA 
GATGATGTGT TCAAAAGAAA TGGTGCCCTG CTTGGAAACC ATGTCAATCA TGTTAATAGT 
GATAGGAGAG ATTCCCTTCA GCAGACCAAT ACCACCCACC GTCCCCTGCA TGTCCAAAGG 
CCTTCAATTC CACCTGCAAG TGATACTGAG AAACCGCTGT TTCCTCCAGC AGGAAATTCG 
GTGTGTCATA ACCATCATAA CCATAATTCC ATAGGAAAGC AAGTTCCCAC CTCAACAAAT 
GCCAATCTCA ATAATGCCAA TATGTCCAAA GCTGCCCATG GAAAGCGGCC CAGCATTGGG 
AACCTTGAGC ATGTGTCTGA AAATGGGCAT CATTCTTCCC ACAAGCATGA CCGGGAGCCT 
CAGAGAAGGT CCAGTGTGAA AAGAACCCGC TATTATGAAA CTTACATTAG GTCCGACTCA 
GGAGATGAAC AGCTCCCAAC TATTTGCCGG GAAGACCCAG AGATACATGG CTATTTCAGG 
GACCCCCACT GCTTGGGGGA GCAGGAGTAT TTCAGTAGTG AGGAATGCTA CGAGGATGAC 
AGCTCGCCCA CCTGGAGCAG GCAAAACTAT GGCTACTACA GCAGATACCC AGGCAGAAAC 
ATCGACTCTG AGAGGCCCCG AGGCTACCAT CATCCCCAAG GATTCTTGGA GGACGATGAC 
TCGCCCGTTT GCTATGATTC ACGGAGATCT CCAAGGAGAC GCCTACTACC TCCCACCCCA 
GCATCCCACC GGAGATCCTC CTTCAACTTT GAGTGCCTGC GCCGGCAGAG CAGCCAGGAA 
GAGGTCCCGT CGTCTCCCAT CTTCCCCCAT CGCACGGCCC TGCCTCTGCA TCTAATGCAG 
CAACAGATCA TGGCAGTTGC CGGCCTAGAT TCAAGTAAAG CCCAGAAGTA CTCACCGAGT 
CACTCGACCC GGTCGTGGGC CACCCCTCCA GCAACCCCTC CCTACCGGGA CTGGACACCG 
TGCTACACCC CCCTGATCCA AGTGGAGCAG TCAGAGGCCC TGGACCAGGT GAACGGCAGC 
CTGCCGTCCC TGCACCGCAG CTCCTGGTAC ACAGACGAGC CCGACATCTC CTACCGGACT 
TTCACACCAG CCAGCCTGAC TGTCCCCAGC AGCTTCCGGA ACAAAAACAG CGACAAGCAG 
AGGAGTGCGG ACAGCTTGGT GGAGGCAGTC CTGATATCCG AAGGCTTGGG ACGCTATGCA 
AGGGACCCAA AATTTGTGTC AGCAACAAAA CACGAAATCG CTGATGCCTG TGACCTCACC 
ATCGACGAGA TGGAGAGTGC AGCCAGCACC CTGCTTAATG GGAACGTGCG TCCCCGAGCC 
AACGGGGATG TGGGCCCCCT CTCACACCGG CAGGACTATG AGCTACAGGA CTTTGGTCCT 
GGCTACAGCG ACGAAGAGCC AGACCCTGGG AGGGATGAGG AGGACCTGGC GGATGAAATG 
ATATGCATCA CCACCTTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_011534096.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011532398.1 Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X11, mRNA.

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CloneID	OHu56578
Clone ID Related Accession (Same CDS sequence)	XM_011534096.2
Accession Version	XM_011534096.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011534096.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGGG AGCCGCTGCC GCTGTGGGGC TCCCCTCCCC AGCTCTGGCC CGGGCACCAC 
GTGCAGCTTC CACGCCGGCC GGGGCTGGGT CGAGGGAGGA TGGAGCAGCT CCTGGCCAGC 
CCTCCCCTTT GGAGACCGCC AGTGCCCCCC AACCCCTGTC GGCTGGAAGC CTTTCCGGAG 
GCAAACTATG CAAGAGGCAC CAGACTTCCT CTTTCTGGTG AAGGACCAAC TTCTCAGCCG 
AATAGCTCCA AGCAAACTGT CCTGTCTTGG CAAGCTGCAA TCGATGCTGC TAGACAGGCC 
AAGGCTGCCC AAACTATGAG CACCTCTGCA CCCCCACCTG TAGGATCTCT CTCCCAAAGA 
AAACGTCAGC AATACGCCAA GAGCAAAAAA CAGGGTAACT CGTCCAACAG CCGACCTGCC 
CGCGCCCTTT TCTGTTTATC ACTCAATAAC CCCATCCGAA GAGCCTGCAT TAGTATAGTG 
GAATGGAAAC CATTTGACAT ATTTATATTA TTGGCTATTT TTGCCAATTG TGTGGCCTTA 
GCTATTTACA TCCCATTCCC TGAAGATGAT TCTAATTCAA CAAATCATAA CTTGGAAAAA 
GTAGAATATG CCTTCCTGAT TATTTTTACA GTCGAGACAT TTTTGAAGAT TATAGCGTAT 
GGATTATTGC TACATCCTAA TGCTTATGTT AGGAATGGAT GGAATTTACT GGATTTTGTT 
ATAGTAATAG TAGGATTGTT TAGTGTAATT TTGGAACAAT TAACCAAAGA AACAGAAGGC 
GGGAACCACT CAAGCGGCAA ATCTGGAGGC TTTGATGTCA AAGCCCTCCG TGCCTTTCGA 
GTGTTGCGAC CACTTCGACT AGTGTCAGGA GTGCCCAGTT TACAAGTTGT CCTGAACTCC 
ATTATAAAAG CCATGGTTCC CCTCCTTCAC ATAGCCCTTT TGGTATTATT TGTAATCATA 
ATCTATGCTA TTATAGGATT GGAACTTTTT ATTGGAAAAA TGCACAAAAC ATGTTTTTTT 
GCTGACTCAG ATATCGTAGC TGAAGAGGAC CCAGCTCCAT GTGCGTTCTC AGGGAATGGA 
CGCCAGTGTA CTGCCAATGG CACGGAATGT AGGAGTGGCT GGGTTGGCCC GAACGGAGGC 
ATCACCAACT TTGATAACTT TGCCTTTGCC ATGCTTACTG TGTTTCAGTG CATCACCATG 
GAGGGCTGGA CAGATGTGCT CTACTGGGTA AATGATGCGA TAGGATGGGA ATGGCCATGG 
GTGTATTTTG TTAGTCTGAT CATCCTTGGC TCATTTTTCG TCCTTAACCT GGTTCTTGGT 
GTCCTTAGTG GAGAATTCTC AAAGGAAAGA GAGAAGGCAA AAGCACGGGG AGATTTCCAG 
AAGCTCCGGG AGAAGCAGCA GCTGGAGGAG GATCTAAAGG GCTACTTGGA TTGGATCACC 
CAAGCTGAGG ACATCGATCC GGAGAATGAG GAAGAAGGAG GAGAGGAAGG CAAACGAAAT 
ACTAGCATGC CCACCAGCGA GACTGAGTCT GTGAACACAG AGAACGTCAG CGGTGAAGGC 
GAGAACCGAG GCTGCTGTGG AAGTCTCTGT CAAGCCATCT CAAAATCCAA ACTCAGCCGA 
CGCTGGCGTC GCTGGAACCG ATTCAATCGC AGAAGATGTA GGGCCGCCGT GAAGTCTGTC 
ACGTTTTACT GGCTGGTTAT CGTCCTGGTG TTTCTGAACA CCTTAACCAT TTCCTCTGAG 
CACTACAATC AGCCAGATTG GTTGACACAG ATTCAAGATA TTGCCAACAA AGTCCTCTTG 
GCTCTGTTCA CCTGCGAGAT GCTGGTAAAA ATGTACAGCT TGGGCCTCCA AGCATATTTC 
GTCTCTCTTT TCAACCGGTT TGATTGCTTC GTGGTGTGTG GTGGAATCAC TGAGACGATC 
TTGGTGGAAC TGGAAATCAT GTCTCCCCTG GGGATCTCTG TGTTTCGGTG TGTGCGCCTC 
TTAAGAATCT TCAAAGTGAC CAGGCACTGG ACTTCCCTGA GCAACTTAGT GGCATCCTTA 
TTAAACTCCA TGAAGTCCAT CGCTTCGCTG TTGCTTCTGC TTTTTCTCTT CATTATCATC 
TTTTCCTTGC TTGGGATGCA GCTGTTTGGC GGCAAGTTTA ATTTTGATGA AACGCAAACC 
AAGCGGAGCA CCTTTGACAA TTTCCCTCAA GCACTTCTCA CAGTGTTCCA GATCCTGACA 
GGCGAAGACT GGAATGCTGT GATGTACGAT GGCATCATGG CTTACGGGGG CCCATCCTCT 
TCAGGAATGA TCGTCTGCAT CTACTTCATC ATCCTCTTCA TTTGTGGTAA CTATATTCTA 
CTGAATGTCT TCTTGGCCAT CGCTGTAGAC AATTTGGCTG ATGCTGAAAG TCTGAACACT 
GCTCAGAAAG AAGAAGCGGA AGAAAAGGAG AGGAAAAAGA TTGCCAGAAA AGAGAGCCTA 
GAAAATAAAA AGAACAACAA ACCAGAAGTC AACCAGATAG CCAACAGTGA CAACAAGGTT 
ACAATTGATG ACTATAGAGA AGAGGATGAA GACAAGGACC CCTATCCGCC TTGCGATGTG 
CCAGTAGGGG AAGAGGAAGA GGAAGAGGAG GAGGATGAAC CTGAGGTTCC TGCCGGACCC 
CGTCCTCGAA GGATCTCGGA GTTGAACATG AAGGAAAAAA TTGCCCCCAT CCCTGAAGGG 
AGCGCTTTCT TCATTCTTAG CAAGACCAAC CCGATCCGCG TAGGCTGCCA CAAGCTCATC 
AACCACCACA TCTTCACCAA CCTCATCCTT GTCTTCATCA TGCTGAGCAG CGCTGCCCTG 
GCCGCAGAGG ACCCCATCCG CAGCCACTCC TTCCGGAACA CGATACTGGG TTACTTTGAC 
TATGCCTTCA CAGCCATCTT TACTGTTGAG ATCCTGTTGA AGATGACAAC TTTTGGAGCT 
TTCCTCCACA AAGGGGCCTT CTGCAGGAAC TACTTCAATT TGCTGGATAT GCTGGTGGTT 
GGGGTGTCTC TGGTGTCATT TGGGATTCAA TCCAGTGCCA TCTCCGTTGT GAAGATTCTG 
AGGGTCTTAA GGGTCCTGCG TCCCCTCAGG GCCATCAACA GAGCAAAAGG ACTTAAGCAC 
GTGGTCCAGT GCGTCTTCGT GGCCATCCGG ACCATCGGCA ACATCATGAT CGTCACCACC 
CTCCTGCAGT TCATGTTTGC CTGTATCGGG GTCCAGTTGT TCAAGGGGAA GTTCTATCGC 
TGTACGGATG AAGCCAAAAG TAACCCTGAA GAATGCAGGG GACTTTTCAT CCTCTACAAG 
GATGGGGATG TTGACAGTCC TGTGGTCCGT GAACGGATCT GGCAAAACAG TGATTTCAAC 
TTCGACAACG TCCTCTCTGC TATGATGGCG CTCTTCACAG TCTCCACGTT TGAGGGCTGG 
CCTGCGTTGC TGTATAAAGC CATCGACTCG AATGGAGAGA ACATCGGCCC AATCTACAAC 
CACCGCGTGG AGATCTCCAT CTTCTTCATC ATCTACATCA TCATTGTAGC TTTCTTCATG 
ATGAACATCT TTGTGGGCTT TGTCATCGTT ACATTTCAGG AACAAGGAGA AAAAGAGTAT 
AAGAACTGTG AGCTGGACAA AAATCAGCGT CAGTGTGTTG AATACGCCTT GAAAGCACGT 
CCCTTGCGGA GATACATCCC CAAAAACCCC TACCAGTACA AGTTCTGGTA CGTGGTGAAC 
TCTTCGCCTT TCGAATACAT GATGTTTGTC CTCATCATGC TCAACACACT CTGCTTGGCC 
ATGCAGCACT ACGAGCAGTC CAAGATGTTC AATGATGCCA TGGACATTCT GAACATGGTC 
TTCACCGGGG TGTTCACCGT CGAGATGGTT TTGAAAGTCA TCGCATTTAA GCCTAAGGGG 
TATTTTAGTG ACGCCTGGAA CACGTTTGAC TCCCTCATCG TAATCGGCAG CATTATAGAC 
GTGGCCCTCA GCGAAGCAGA CAACTCTGAA GAGAGCAATA GAATCTCCAT CACCTTTTTC 
CGTCTTTTCC GAGTGATGCG ATTGGTGAAG CTTCTCAGCA GGGGGGAAGG CATCCGGACA 
TTGCTGTGGA CTTTTATTAA GTCCTTTCAG GCGCTCCCGT ATGTGGCCCT CCTCATAGCC 
ATGCTGTTCT TCATCTATGC GGTCATTGGC ATGCAGATGT TTGGGAAAGT TGCCATGAGA 
GATAACAACC AGATCAATAG GAACAATAAC TTCCAGACGT TTCCCCAGGC GGTGCTGCTG 
CTCTTCAGGT GTGCAACAGG TGAGGCCTGG CAGGAGATCA TGCTGGCCTG TCTCCCAGGG 
AAGCTCTGTG ACCCTGAGTC AGATTACAAC CCCGGGGAGG AGTATACATG TGGGAGCAAC 
TTTGCCATTG TCTATTTCAT CAGTTTTTAC ATGCTCTGTG CATTTCTGAT CATCAATCTG 
TTTGTGGCTG TCATCATGGA TAATTTCGAC TATCTGACCC GGGACTGGTC TATTTTGGGG 
CCTCACCATT TAGATGAATT CAAAAGAATA TGGTCAGAAT ATGACCCTGA GGCAAAGGGA 
AGGATAAAAC ACCTTGATGT GGTCACTCTG CTTCGACGCA TCCAGCCTCC CCTGGGGTTT 
GGGAAGTTAT GTCCACACAG GGTAGCGTGC AAGAGATTAG TTGCCATGAA CATGCCTCTC 
AACAGTGACG GGACAGTCAT GTTTAATGCA ACCCTGTTTG CTTTGGTTCG AACGGCTCTT 
AAGATCAAGA CCGAAGGGAA CCTGGAGCAA GCTAATGAAG AACTTCGGGC TGTGATAAAG 
AAAATTTGGA AGAAAACCAG CATGAAATTA CTTGACCAAG TTGTCCCTCC AGCTGGTGAT 
GATGAGGTAA CCGTGGGGAA GTTCTATGCC ACTTTCCTGA TACAGGACTA CTTTAGGAAA 
TTCAAGAAAC GGAAAGAACA AGGACTGGTG GGAAAGTACC CTGCGAAGAA CACCACAATT 
GCCCTACAGG CGGGATTAAG GACACTGCAT GACATTGGGC CAGAAATCCG GCGTGCTATA 
TCGTGTGATT TGCAAGATGA CGAGCCTGAG GAAACAAAAC GAGAAGAAGA AGATGATGTG 
TTCAAAAGAA ATGGTGCCCT GCTTGGAAAC CATGTCAATC ATGTTAATAG TGATAGGAGA 
GATTCCCTTC AGCAGACCAA TACCACCCAC CGTCCCCTGC ATGTCCAAAG GCCTTCAATT 
CCACCTGCAA GTGATACTGA GAAACCGCTG TTTCCTCCAG CAGGAAATTC GGTGTGTCAT 
AACCATCATA ACCATAATTC CATAGGAAAG CAAGTTCCCA CCTCAACAAA TGCCAATCTC 
AATAATGCCA ATATGTCCAA AGCTGCCCAT GGAAAGCGGC CCAGCATTGG GAACCTTGAG 
CATGTGTCTG AAAATGGGCA TCATTCTTCC CACAAGCATG ACCGGGAGCC TCAGAGAAGG 
TCCAGTGTGA AAAGAACCCG CTATTATGAA ACTTACATTA GGTCCGACTC AGGAGATGAA 
CAGCTCCCAA CTATTTGCCG GGAAGACCCA GAGATACATG GCTATTTCAG GGACCCCCAC 
TGCTTGGGGG AGCAGGAGTA TTTCAGTAGT GAGGAATGCT ACGAGGATGA CAGCTCGCCC 
ACCTGGAGCA GGCAAAACTA TGGCTACTAC AGCAGATACC CAGGCAGAAA CATCGACTCT 
GAGAGGCCCC GAGGCTACCA TCATCCCCAA GGATTCTTGG AGGACGATGA CTCGCCCGTT 
TGCTATGATT CACGGAGATC TCCAAGGAGA CGCCTACTAC CTCCCACCCC AGCATCCCAC 
CGGAGATCCT CCTTCAACTT TGAGTGCCTG CGCCGGCAGA GCAGCCAGGA AGAGGTCCCG 
TCGTCTCCCA TCTTCCCCCA TCGCACGGCC CTGCCTCTGC ATCTAATGCA GCAACAGATC 
ATGGCAGTTG CCGGCCTAGA TTCAAGTAAA GCCCAGAAGT ACTCACCGAG TCACTCGACC 
CGGTCGTGGG CCACCCCTCC AGCAACCCCT CCCTACCGGG ACTGGACACC GTGCTACACC 
CCCCTGATCC AAGTGGAGCA GTCAGAGGCC CTGGACCAGG TGAACGGCAG CCTGCCGTCC 
CTGCACCGCA GCTCCTGGTA CACAGACGAG CCCGACATCT CCTACCGGAC TTTCACACCA 
GCCAGCCTGA CTGTCCCCAG CAGCTTCCGG AACAAAAACA GCGACAAGCA GAGGAGTGCG 
GACAGCTTGG TGGAGGCAGT CCTGATATCC GAAGGCTTGG GACGCTATGC AAGGGACCCA 
AAATTTGTGT CAGCAACAAA ACACGAAATC GCTGATGCCT GTGACCTCAC CATCGACGAG 
ATGGAGAGTG CAGCCAGCAC CCTGCTTAAT GGGAACGTGC GTCCCCGAGC CAACGGGGAT 
GTGGGCCCCC TCTCACACCG GCAGGACTAT GAGCTACAGG ACTTTGGTCC TGGCTACAGC 
GACGAAGAGC CAGACCCTGG GAGGGATGAG GAGGACCTGG CGGATGAAAT GATATGCATC 
ACCACCTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011532398.1
CDS	16..6567
Translation	MPGEPLPLWGSPPQLWPGHHVQLPRRPGLGRGRMEQLLASPPLWRPPVPPNPCRLEAFPEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGENRGCCGSLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENIGPIYNHRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEDDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGNLEHVSENGHHSSHKHDREPQRRSSVKRTRYYETYIRSDSGDEQLPTICREDPEIHGYFRDPHCLGEQEYFSSEECYEDDSSPTWSRQNYGYYSRYPGRNIDSERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPASHRRSSFNFECLRRQSSQEEVPSSPIFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVEQSEALDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGNVRPRANGDVGPLSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_011534096.2
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011534096.2

ORF Insert Sequence:

ATGCCCGGGG AGCCGCTGCC GCTGTGGGGC TCCCCTCCCC AGCTCTGGCC CGGGCACCAC 
GTGCAGCTTC CACGCCGGCC GGGGCTGGGT CGAGGGAGGA TGGAGCAGCT CCTGGCCAGC 
CCTCCCCTTT GGAGACCGCC AGTGCCCCCC AACCCCTGTC GGCTGGAAGC CTTTCCGGAG 
GCAAACTATG CAAGAGGCAC CAGACTTCCT CTTTCTGGTG AAGGACCAAC TTCTCAGCCG 
AATAGCTCCA AGCAAACTGT CCTGTCTTGG CAAGCTGCAA TCGATGCTGC TAGACAGGCC 
AAGGCTGCCC AAACTATGAG CACCTCTGCA CCCCCACCTG TAGGATCTCT CTCCCAAAGA 
AAACGTCAGC AATACGCCAA GAGCAAAAAA CAGGGTAACT CGTCCAACAG CCGACCTGCC 
CGCGCCCTTT TCTGTTTATC ACTCAATAAC CCCATCCGAA GAGCCTGCAT TAGTATAGTG 
GAATGGAAAC CATTTGACAT ATTTATATTA TTGGCTATTT TTGCCAATTG TGTGGCCTTA 
GCTATTTACA TCCCATTCCC TGAAGATGAT TCTAATTCAA CAAATCATAA CTTGGAAAAA 
GTAGAATATG CCTTCCTGAT TATTTTTACA GTCGAGACAT TTTTGAAGAT TATAGCGTAT 
GGATTATTGC TACATCCTAA TGCTTATGTT AGGAATGGAT GGAATTTACT GGATTTTGTT 
ATAGTAATAG TAGGATTGTT TAGTGTAATT TTGGAACAAT TAACCAAAGA AACAGAAGGC 
GGGAACCACT CAAGCGGCAA ATCTGGAGGC TTTGATGTCA AAGCCCTCCG TGCCTTTCGA 
GTGTTGCGAC CACTTCGACT AGTGTCAGGA GTGCCCAGTT TACAAGTTGT CCTGAACTCC 
ATTATAAAAG CCATGGTTCC CCTCCTTCAC ATAGCCCTTT TGGTATTATT TGTAATCATA 
ATCTATGCTA TTATAGGATT GGAACTTTTT ATTGGAAAAA TGCACAAAAC ATGTTTTTTT 
GCTGACTCAG ATATCGTAGC TGAAGAGGAC CCAGCTCCAT GTGCGTTCTC AGGGAATGGA 
CGCCAGTGTA CTGCCAATGG CACGGAATGT AGGAGTGGCT GGGTTGGCCC GAACGGAGGC 
ATCACCAACT TTGATAACTT TGCCTTTGCC ATGCTTACTG TGTTTCAGTG CATCACCATG 
GAGGGCTGGA CAGATGTGCT CTACTGGGTA AATGATGCGA TAGGATGGGA ATGGCCATGG 
GTGTATTTTG TTAGTCTGAT CATCCTTGGC TCATTTTTCG TCCTTAACCT GGTTCTTGGT 
GTCCTTAGTG GAGAATTCTC AAAGGAAAGA GAGAAGGCAA AAGCACGGGG AGATTTCCAG 
AAGCTCCGGG AGAAGCAGCA GCTGGAGGAG GATCTAAAGG GCTACTTGGA TTGGATCACC 
CAAGCTGAGG ACATCGATCC GGAGAATGAG GAAGAAGGAG GAGAGGAAGG CAAACGAAAT 
ACTAGCATGC CCACCAGCGA GACTGAGTCT GTGAACACAG AGAACGTCAG CGGTGAAGGC 
GAGAACCGAG GCTGCTGTGG AAGTCTCTGT CAAGCCATCT CAAAATCCAA ACTCAGCCGA 
CGCTGGCGTC GCTGGAACCG ATTCAATCGC AGAAGATGTA GGGCCGCCGT GAAGTCTGTC 
ACGTTTTACT GGCTGGTTAT CGTCCTGGTG TTTCTGAACA CCTTAACCAT TTCCTCTGAG 
CACTACAATC AGCCAGATTG GTTGACACAG ATTCAAGATA TTGCCAACAA AGTCCTCTTG 
GCTCTGTTCA CCTGCGAGAT GCTGGTAAAA ATGTACAGCT TGGGCCTCCA AGCATATTTC 
GTCTCTCTTT TCAACCGGTT TGATTGCTTC GTGGTGTGTG GTGGAATCAC TGAGACGATC 
TTGGTGGAAC TGGAAATCAT GTCTCCCCTG GGGATCTCTG TGTTTCGGTG TGTGCGCCTC 
TTAAGAATCT TCAAAGTGAC CAGGCACTGG ACTTCCCTGA GCAACTTAGT GGCATCCTTA 
TTAAACTCCA TGAAGTCCAT CGCTTCGCTG TTGCTTCTGC TTTTTCTCTT CATTATCATC 
TTTTCCTTGC TTGGGATGCA GCTGTTTGGC GGCAAGTTTA ATTTTGATGA AACGCAAACC 
AAGCGGAGCA CCTTTGACAA TTTCCCTCAA GCACTTCTCA CAGTGTTCCA GATCCTGACA 
GGCGAAGACT GGAATGCTGT GATGTACGAT GGCATCATGG CTTACGGGGG CCCATCCTCT 
TCAGGAATGA TCGTCTGCAT CTACTTCATC ATCCTCTTCA TTTGTGGTAA CTATATTCTA 
CTGAATGTCT TCTTGGCCAT CGCTGTAGAC AATTTGGCTG ATGCTGAAAG TCTGAACACT 
GCTCAGAAAG AAGAAGCGGA AGAAAAGGAG AGGAAAAAGA TTGCCAGAAA AGAGAGCCTA 
GAAAATAAAA AGAACAACAA ACCAGAAGTC AACCAGATAG CCAACAGTGA CAACAAGGTT 
ACAATTGATG ACTATAGAGA AGAGGATGAA GACAAGGACC CCTATCCGCC TTGCGATGTG 
CCAGTAGGGG AAGAGGAAGA GGAAGAGGAG GAGGATGAAC CTGAGGTTCC TGCCGGACCC 
CGTCCTCGAA GGATCTCGGA GTTGAACATG AAGGAAAAAA TTGCCCCCAT CCCTGAAGGG 
AGCGCTTTCT TCATTCTTAG CAAGACCAAC CCGATCCGCG TAGGCTGCCA CAAGCTCATC 
AACCACCACA TCTTCACCAA CCTCATCCTT GTCTTCATCA TGCTGAGCAG CGCTGCCCTG 
GCCGCAGAGG ACCCCATCCG CAGCCACTCC TTCCGGAACA CGATACTGGG TTACTTTGAC 
TATGCCTTCA CAGCCATCTT TACTGTTGAG ATCCTGTTGA AGATGACAAC TTTTGGAGCT 
TTCCTCCACA AAGGGGCCTT CTGCAGGAAC TACTTCAATT TGCTGGATAT GCTGGTGGTT 
GGGGTGTCTC TGGTGTCATT TGGGATTCAA TCCAGTGCCA TCTCCGTTGT GAAGATTCTG 
AGGGTCTTAA GGGTCCTGCG TCCCCTCAGG GCCATCAACA GAGCAAAAGG ACTTAAGCAC 
GTGGTCCAGT GCGTCTTCGT GGCCATCCGG ACCATCGGCA ACATCATGAT CGTCACCACC 
CTCCTGCAGT TCATGTTTGC CTGTATCGGG GTCCAGTTGT TCAAGGGGAA GTTCTATCGC 
TGTACGGATG AAGCCAAAAG TAACCCTGAA GAATGCAGGG GACTTTTCAT CCTCTACAAG 
GATGGGGATG TTGACAGTCC TGTGGTCCGT GAACGGATCT GGCAAAACAG TGATTTCAAC 
TTCGACAACG TCCTCTCTGC TATGATGGCG CTCTTCACAG TCTCCACGTT TGAGGGCTGG 
CCTGCGTTGC TGTATAAAGC CATCGACTCG AATGGAGAGA ACATCGGCCC AATCTACAAC 
CACCGCGTGG AGATCTCCAT CTTCTTCATC ATCTACATCA TCATTGTAGC TTTCTTCATG 
ATGAACATCT TTGTGGGCTT TGTCATCGTT ACATTTCAGG AACAAGGAGA AAAAGAGTAT 
AAGAACTGTG AGCTGGACAA AAATCAGCGT CAGTGTGTTG AATACGCCTT GAAAGCACGT 
CCCTTGCGGA GATACATCCC CAAAAACCCC TACCAGTACA AGTTCTGGTA CGTGGTGAAC 
TCTTCGCCTT TCGAATACAT GATGTTTGTC CTCATCATGC TCAACACACT CTGCTTGGCC 
ATGCAGCACT ACGAGCAGTC CAAGATGTTC AATGATGCCA TGGACATTCT GAACATGGTC 
TTCACCGGGG TGTTCACCGT CGAGATGGTT TTGAAAGTCA TCGCATTTAA GCCTAAGGGG 
TATTTTAGTG ACGCCTGGAA CACGTTTGAC TCCCTCATCG TAATCGGCAG CATTATAGAC 
GTGGCCCTCA GCGAAGCAGA CAACTCTGAA GAGAGCAATA GAATCTCCAT CACCTTTTTC 
CGTCTTTTCC GAGTGATGCG ATTGGTGAAG CTTCTCAGCA GGGGGGAAGG CATCCGGACA 
TTGCTGTGGA CTTTTATTAA GTCCTTTCAG GCGCTCCCGT ATGTGGCCCT CCTCATAGCC 
ATGCTGTTCT TCATCTATGC GGTCATTGGC ATGCAGATGT TTGGGAAAGT TGCCATGAGA 
GATAACAACC AGATCAATAG GAACAATAAC TTCCAGACGT TTCCCCAGGC GGTGCTGCTG 
CTCTTCAGGT GTGCAACAGG TGAGGCCTGG CAGGAGATCA TGCTGGCCTG TCTCCCAGGG 
AAGCTCTGTG ACCCTGAGTC AGATTACAAC CCCGGGGAGG AGTATACATG TGGGAGCAAC 
TTTGCCATTG TCTATTTCAT CAGTTTTTAC ATGCTCTGTG CATTTCTGAT CATCAATCTG 
TTTGTGGCTG TCATCATGGA TAATTTCGAC TATCTGACCC GGGACTGGTC TATTTTGGGG 
CCTCACCATT TAGATGAATT CAAAAGAATA TGGTCAGAAT ATGACCCTGA GGCAAAGGGA 
AGGATAAAAC ACCTTGATGT GGTCACTCTG CTTCGACGCA TCCAGCCTCC CCTGGGGTTT 
GGGAAGTTAT GTCCACACAG GGTAGCGTGC AAGAGATTAG TTGCCATGAA CATGCCTCTC 
AACAGTGACG GGACAGTCAT GTTTAATGCA ACCCTGTTTG CTTTGGTTCG AACGGCTCTT 
AAGATCAAGA CCGAAGGGAA CCTGGAGCAA GCTAATGAAG AACTTCGGGC TGTGATAAAG 
AAAATTTGGA AGAAAACCAG CATGAAATTA CTTGACCAAG TTGTCCCTCC AGCTGGTGAT 
GATGAGGTAA CCGTGGGGAA GTTCTATGCC ACTTTCCTGA TACAGGACTA CTTTAGGAAA 
TTCAAGAAAC GGAAAGAACA AGGACTGGTG GGAAAGTACC CTGCGAAGAA CACCACAATT 
GCCCTACAGG CGGGATTAAG GACACTGCAT GACATTGGGC CAGAAATCCG GCGTGCTATA 
TCGTGTGATT TGCAAGATGA CGAGCCTGAG GAAACAAAAC GAGAAGAAGA AGATGATGTG 
TTCAAAAGAA ATGGTGCCCT GCTTGGAAAC CATGTCAATC ATGTTAATAG TGATAGGAGA 
GATTCCCTTC AGCAGACCAA TACCACCCAC CGTCCCCTGC ATGTCCAAAG GCCTTCAATT 
CCACCTGCAA GTGATACTGA GAAACCGCTG TTTCCTCCAG CAGGAAATTC GGTGTGTCAT 
AACCATCATA ACCATAATTC CATAGGAAAG CAAGTTCCCA CCTCAACAAA TGCCAATCTC 
AATAATGCCA ATATGTCCAA AGCTGCCCAT GGAAAGCGGC CCAGCATTGG GAACCTTGAG 
CATGTGTCTG AAAATGGGCA TCATTCTTCC CACAAGCATG ACCGGGAGCC TCAGAGAAGG 
TCCAGTGTGA AAAGAACCCG CTATTATGAA ACTTACATTA GGTCCGACTC AGGAGATGAA 
CAGCTCCCAA CTATTTGCCG GGAAGACCCA GAGATACATG GCTATTTCAG GGACCCCCAC 
TGCTTGGGGG AGCAGGAGTA TTTCAGTAGT GAGGAATGCT ACGAGGATGA CAGCTCGCCC 
ACCTGGAGCA GGCAAAACTA TGGCTACTAC AGCAGATACC CAGGCAGAAA CATCGACTCT 
GAGAGGCCCC GAGGCTACCA TCATCCCCAA GGATTCTTGG AGGACGATGA CTCGCCCGTT 
TGCTATGATT CACGGAGATC TCCAAGGAGA CGCCTACTAC CTCCCACCCC AGCATCCCAC 
CGGAGATCCT CCTTCAACTT TGAGTGCCTG CGCCGGCAGA GCAGCCAGGA AGAGGTCCCG 
TCGTCTCCCA TCTTCCCCCA TCGCACGGCC CTGCCTCTGC ATCTAATGCA GCAACAGATC 
ATGGCAGTTG CCGGCCTAGA TTCAAGTAAA GCCCAGAAGT ACTCACCGAG TCACTCGACC 
CGGTCGTGGG CCACCCCTCC AGCAACCCCT CCCTACCGGG ACTGGACACC GTGCTACACC 
CCCCTGATCC AAGTGGAGCA GTCAGAGGCC CTGGACCAGG TGAACGGCAG CCTGCCGTCC 
CTGCACCGCA GCTCCTGGTA CACAGACGAG CCCGACATCT CCTACCGGAC TTTCACACCA 
GCCAGCCTGA CTGTCCCCAG CAGCTTCCGG AACAAAAACA GCGACAAGCA GAGGAGTGCG 
GACAGCTTGG TGGAGGCAGT CCTGATATCC GAAGGCTTGG GACGCTATGC AAGGGACCCA 
AAATTTGTGT CAGCAACAAA ACACGAAATC GCTGATGCCT GTGACCTCAC CATCGACGAG 
ATGGAGAGTG CAGCCAGCAC CCTGCTTAAT GGGAACGTGC GTCCCCGAGC CAACGGGGAT 
GTGGGCCCCC TCTCACACCG GCAGGACTAT GAGCTACAGG ACTTTGGTCC TGGCTACAGC 
GACGAAGAGC CAGACCCTGG GAGGGATGAG GAGGACCTGG CGGATGAAAT GATATGCATC 
ACCACCTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_011534097.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011532399.1 Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X13, mRNA.

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CloneID	OHu56579
Clone ID Related Accession (Same CDS sequence)	XM_011534097.2
Accession Version	XM_011534097.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011534097.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTACTT CTGCCGAAAA CCTAGCAGTC ACAGCAGTCA GCCGAAATAG GATTTGTGAA 
AAAGTAGAAT ATGCCTTCCT GATTATTTTT ACAGTCGAGA CATTTTTGAA GATTATAGCG 
TATGGATTAT TGCTACATCC TAATGCTTAT GTTAGGAATG GATGGAATTT ACTGGATTTT 
GTTATAGTAA TAGTAGGATT GTTTAGTGTA ATTTTGGAAC AATTAACCAA AGAAACAGAA 
GGCGGGAACC ACTCAAGCGG CAAATCTGGA GGCTTTGATG TCAAAGCCCT CCGTGCCTTT 
CGAGTGTTGC GACCACTTCG ACTAGTGTCA GGAGTGCCCA GTTTACAAGT TGTCCTGAAC 
TCCATTATAA AAGCCATGGT TCCCCTCCTT CACATAGCCC TTTTGGTATT ATTTGTAATC 
ATAATCTATG CTATTATAGG ATTGGAACTT TTTATTGGAA AAATGCACAA AACATGTTTT 
TTTGCTGACT CAGATATCGT AGCTGAAGAG GACCCAGCTC CATGTGCGTT CTCAGGGAAT 
GGACGCCAGT GTACTGCCAA TGGCACGGAA TGTAGGAGTG GCTGGGTTGG CCCGAACGGA 
GGCATCACCA ACTTTGATAA CTTTGCCTTT GCCATGCTTA CTGTGTTTCA GTGCATCACC 
ATGGAGGGCT GGACAGATGT GCTCTACTGG GTAAATGATG CGATAGGATG GGAATGGCCA 
TGGGTGTATT TTGTTAGTCT GATCATCCTT GGCTCATTTT TCGTCCTTAA CCTGGTTCTT 
GGTGTCCTTA GTGGAGAATT CTCAAAGGAA AGAGAGAAGG CAAAAGCACG GGGAGATTTC 
CAGAAGCTCC GGGAGAAGCA GCAGCTGGAG GAGGATCTAA AGGGCTACTT GGATTGGATC 
ACCCAAGCTG AGGACATCGA TCCGGAGAAT GAGGAAGAAG GAGGAGAGGA AGGCAAACGA 
AATACTAGCA TGCCCACCAG CGAGACTGAG TCTGTGAACA CAGAGAACGT CAGCGGTGAA 
GGCGAGAACC GAGGCTGCTG TGGAAGTCTC TGGTGCTGGT GGAGACGGAG AGGCGCGGCC 
AAGGCGGGGC CCTCTGGGTG TCGGCGGTGG GGTCAAGCCA TCTCAAAATC CAAACTCAGC 
CGACGCTGGC GTCGCTGGAA CCGATTCAAT CGCAGAAGAT GTAGGGCCGC CGTGAAGTCT 
GTCACGTTTT ACTGGCTGGT TATCGTCCTG GTGTTTCTGA ACACCTTAAC CATTTCCTCT 
GAGCACTACA ATCAGCCAGA TTGGTTGACA CAGATTCAAG ATATTGCCAA CAAAGTCCTC 
TTGGCTCTGT TCACCTGCGA GATGCTGGTA AAAATGTACA GCTTGGGCCT CCAAGCATAT 
TTCGTCTCTC TTTTCAACCG GTTTGATTGC TTCGTGGTGT GTGGTGGAAT CACTGAGACG 
ATCTTGGTGG AACTGGAAAT CATGTCTCCC CTGGGGATCT CTGTGTTTCG GTGTGTGCGC 
CTCTTAAGAA TCTTCAAAGT GACCAGGCAC TGGACTTCCC TGAGCAACTT AGTGGCATCC 
TTATTAAACT CCATGAAGTC CATCGCTTCG CTGTTGCTTC TGCTTTTTCT CTTCATTATC 
ATCTTTTCCT TGCTTGGGAT GCAGCTGTTT GGCGGCAAGT TTAATTTTGA TGAAACGCAA 
ACCAAGCGGA GCACCTTTGA CAATTTCCCT CAAGCACTTC TCACAGTGTT CCAGATCCTG 
ACAGGCGAAG ACTGGAATGC TGTGATGTAC GATGGCATCA TGGCTTACGG GGGCCCATCC 
TCTTCAGGAA TGATCGTCTG CATCTACTTC ATCATCCTCT TCATTTGTGG TAACTATATT 
CTACTGAATG TCTTCTTGGC CATCGCTGTA GACAATTTGG CTGATGCTGA AAGTCTGAAC 
ACTGCTCAGA AAGAAGAAGC GGAAGAAAAG GAGAGGAAAA AGATTGCCAG AAAAGAGAGC 
CTAGAAAATA AAAAGAACAA CAAACCAGAA GTCAACCAGA TAGCCAACAG TGACAACAAG 
GTTACAATTG ATGACTATAG AGAAGAGGAT GAAGACAAGG ACCCCTATCC GCCTTGCGAT 
GTGCCAGTAG GGGAAGAGGA AGAGGAAGAG GAGGAGGATG AACCTGAGGT TCCTGCCGGA 
CCCCGTCCTC GAAGGATCTC GGAGTTGAAC ATGAAGGAAA AAATTGCCCC CATCCCTGAA 
GGGAGCGCTT TCTTCATTCT TAGCAAGACC AACCCGATCC GCGTAGGCTG CCACAAGCTC 
ATCAACCACC ACATCTTCAC CAACCTCATC CTTGTCTTCA TCATGCTGAG CAGCGCTGCC 
CTGGCCGCAG AGGACCCCAT CCGCAGCCAC TCCTTCCGGA ACACGATACT GGGTTACTTT 
GACTATGCCT TCACAGCCAT CTTTACTGTT GAGATCCTGT TGAAGATGAC AACTTTTGGA 
GCTTTCCTCC ACAAAGGGGC CTTCTGCAGG AACTACTTCA ATTTGCTGGA TATGCTGGTG 
GTTGGGGTGT CTCTGGTGTC ATTTGGGATT CAATCCAGTG CCATCTCCGT TGTGAAGATT 
CTGAGGGTCT TAAGGGTCCT GCGTCCCCTC AGGGCCATCA ACAGAGCAAA AGGACTTAAG 
CACGTGGTCC AGTGCGTCTT CGTGGCCATC CGGACCATCG GCAACATCAT GATCGTCACC 
ACCCTCCTGC AGTTCATGTT TGCCTGTATC GGGGTCCAGT TGTTCAAGGG GAAGTTCTAT 
CGCTGTACGG ATGAAGCCAA AAGTAACCCT GAAGAATGCA GGGGACTTTT CATCCTCTAC 
AAGGATGGGG ATGTTGACAG TCCTGTGGTC CGTGAACGGA TCTGGCAAAA CAGTGATTTC 
AACTTCGACA ACGTCCTCTC TGCTATGATG GCGCTCTTCA CAGTCTCCAC GTTTGAGGGC 
TGGCCTGCGT TGCTGTATAA AGCCATCGAC TCGAATGGAG AGAACATCGG CCCAATCTAC 
AACCACCGCG TGGAGATCTC CATCTTCTTC ATCATCTACA TCATCATTGT AGCTTTCTTC 
ATGATGAACA TCTTTGTGGG CTTTGTCATC GTTACATTTC AGGAACAAGG AGAAAAAGAG 
TATAAGAACT GTGAGCTGGA CAAAAATCAG CGTCAGTGTG TTGAATACGC CTTGAAAGCA 
CGTCCCTTGC GGAGATACAT CCCCAAAAAC CCCTACCAGT ACAAGTTCTG GTACGTGGTG 
AACTCTTCGC CTTTCGAATA CATGATGTTT GTCCTCATCA TGCTCAACAC ACTCTGCTTG 
GCCATGCAGC ACTACGAGCA GTCCAAGATG TTCAATGATG CCATGGACAT TCTGAACATG 
GTCTTCACCG GGGTGTTCAC CGTCGAGATG GTTTTGAAAG TCATCGCATT TAAGCCTAAG 
GGGTATTTTA GTGACGCCTG GAACACGTTT GACTCCCTCA TCGTAATCGG CAGCATTATA 
GACGTGGCCC TCAGCGAAGC AGACCACTAT TTCACTGATG CATGGAACAC TTTTGATGCC 
TTAATTGTTG TTGGTAGCGT CGTTGATATT GCTATAACTG AAGTGAATCC AACTGAAAGT 
GAAAATGTCC CTGTCCCAAC TGCTACACCT GGGAACTCTG AAGAGAGCAA TAGAATCTCC 
ATCACCTTTT TCCGTCTTTT CCGAGTGATG CGATTGGTGA AGCTTCTCAG CAGGGGGGAA 
GGCATCCGGA CATTGCTGTG GACTTTTATT AAGTCCTTTC AGGCGCTCCC GTATGTGGCC 
CTCCTCATAG CCATGCTGTT CTTCATCTAT GCGGTCATTG GCATGCAGAT GTTTGGGAAA 
GTTGCCATGA GAGATAACAA CCAGATCAAT AGGAACAATA ACTTCCAGAC GTTTCCCCAG 
GCGGTGCTGC TGCTCTTCAG GTGTGCAACA GGTGAGGCCT GGCAGGAGAT CATGCTGGCC 
TGTCTCCCAG GGAAGCTCTG TGACCCTGAG TCAGATTACA ACCCCGGGGA GGAGTATACA 
TGTGGGAGCA ACTTTGCCAT TGTCTATTTC ATCAGTTTTT ACATGCTCTG TGCATTTCTG 
ATCATCAATC TGTTTGTGGC TGTCATCATG GATAATTTCG ACTATCTGAC CCGGGACTGG 
TCTATTTTGG GGCCTCACCA TTTAGATGAA TTCAAAAGAA TATGGTCAGA ATATGACCCT 
GAGGCAAAGG GAAGGATAAA ACACCTTGAT GTGGTCACTC TGCTTCGACG CATCCAGCCT 
CCCCTGGGGT TTGGGAAGTT ATGTCCACAC AGGGTAGCGT GCAAGAGATT AGTTGCCATG 
AACATGCCTC TCAACAGTGA CGGGACAGTC ATGTTTAATG CAACCCTGTT TGCTTTGGTT 
CGAACGGCTC TTAAGATCAA GACCGAAGGG AACCTGGAGC AAGCTAATGA AGAACTTCGG 
GCTGTGATAA AGAAAATTTG GAAGAAAACC AGCATGAAAT TACTTGACCA AGTTGTCCCT 
CCAGCTGGTG ATGATGAGGT AACCGTGGGG AAGTTCTATG CCACTTTCCT GATACAGGAC 
TACTTTAGGA AATTCAAGAA ACGGAAAGAA CAAGGACTGG TGGGAAAGTA CCCTGCGAAG 
AACACCACAA TTGCCCTACA GGCGGGATTA AGGACACTGC ATGACATTGG GCCAGAAATC 
CGGCGTGCTA TATCGTGTGA TTTGCAAGAT GACGAGCCTG AGGAAACAAA ACGAGAAGAA 
GAAGATGATG TGTTCAAAAG AAATGGTGCC CTGCTTGGAA ACCATGTCAA TCATGTTAAT 
AGTGATAGGA GAGATTCCCT TCAGCAGACC AATACCACCC ACCGTCCCCT GCATGTCCAA 
AGGCCTTCAA TTCCACCTGC AAGTGATACT GAGAAACCGC TGTTTCCTCC AGCAGGAAAT 
TCGGTGTGTC ATAACCATCA TAACCATAAT TCCATAGGAA AGCAAGTTCC CACCTCAACA 
AATGCCAATC TCAATAATGC CAATATGTCC AAAGCTGCCC ATGGAAAGCG GCCCAGCATT 
GGGAACCTTG AGCATGTGTC TGAAAATGGG CATCATTCTT CCCACAAGCA TGACCGGGAG 
CCTCAGAGAA GGTCCAGTGT GAAAAGAACC CGCTATTATG AAACTTACAT TAGGTCCGAC 
TCAGGAGATG AACAGCTCCC AACTATTTGC CGGGAAGACC CAGAGATACA TGGCTATTTC 
AGGGACCCCC ACTGCTTGGG GGAGCAGGAG TATTTCAGTA GTGAGGAATG CTACGAGGAT 
GACAGCTCGC CCACCTGGAG CAGGCAAAAC TATGGCTACT ACAGCAGATA CCCAGGCAGA 
AACATCGACT CTGAGAGGCC CCGAGGCTAC CATCATCCCC AAGGATTCTT GGAGGACGAT 
GACTCGCCCG TTTGCTATGA TTCACGGAGA TCTCCAAGGA GACGCCTACT ACCTCCCACC 
CCAGCATCCC ACCGGAGATC CTCCTTCAAC TTTGAGTGCC TGCGCCGGCA GAGCAGCCAG 
GAAGAGGTCC CGTCGTCTCC CATCTTCCCC CATCGCACGG CCCTGCCTCT GCATCTAATG 
CAGCAACAGA TCATGGCAGT TGCCGGCCTA GATTCAAGTA AAGCCCAGAA GTACTCACCG 
AGTCACTCGA CCCGGTCGTG GGCCACCCCT CCAGCAACCC CTCCCTACCG GGACTGGACA 
CCGTGCTACA CCCCCCTGAT CCAAGTGGAG CAGTCAGAGG CCCTGGACCA GGTGAACGGC 
AGCCTGCCGT CCCTGCACCG CAGCTCCTGG TACACAGACG AGCCCGACAT CTCCTACCGG 
ACTTTCACAC CAGCCAGCCT GACTGTCCCC AGCAGCTTCC GGAACAAAAA CAGCGACAAG 
CAGAGGAGTG CGGACAGCTT GGTGGAGGCA GTCCTGATAT CCGAAGGCTT GGGACGCTAT 
GCAAGGGACC CAAAATTTGT GTCAGCAACA AAACACGAAA TCGCTGATGC CTGTGACCTC 
ACCATCGACG AGATGGAGAG TGCAGCCAGC ACCCTGCTTA ATGGGAACGT GCGTCCCCGA 
GCCAACGGGG ATGTGGGCCC CCTCTCACAC CGGCAGGACT ATGAGCTACA GGACTTTGGT 
CCTGGCTACA GCGACGAAGA GCCAGACCCT GGGAGGGATG AGGAGGACCT GGCGGATGAA 
ATGATATGCA TCACCACCTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011532399.1
CDS	1020..7223
Translation	MITSAENLAVTAVSRNRICEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGENRGCCGSLWCWWRRRGAAKAGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENIGPIYNHRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNPTESENVPVPTATPGNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEDDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGNLEHVSENGHHSSHKHDREPQRRSSVKRTRYYETYIRSDSGDEQLPTICREDPEIHGYFRDPHCLGEQEYFSSEECYEDDSSPTWSRQNYGYYSRYPGRNIDSERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPASHRRSSFNFECLRRQSSQEEVPSSPIFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVEQSEALDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGNVRPRANGDVGPLSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_011534097.2
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011534097.2

ORF Insert Sequence:

ATGATTACTT CTGCCGAAAA CCTAGCAGTC ACAGCAGTCA GCCGAAATAG GATTTGTGAA 
AAAGTAGAAT ATGCCTTCCT GATTATTTTT ACAGTCGAGA CATTTTTGAA GATTATAGCG 
TATGGATTAT TGCTACATCC TAATGCTTAT GTTAGGAATG GATGGAATTT ACTGGATTTT 
GTTATAGTAA TAGTAGGATT GTTTAGTGTA ATTTTGGAAC AATTAACCAA AGAAACAGAA 
GGCGGGAACC ACTCAAGCGG CAAATCTGGA GGCTTTGATG TCAAAGCCCT CCGTGCCTTT 
CGAGTGTTGC GACCACTTCG ACTAGTGTCA GGAGTGCCCA GTTTACAAGT TGTCCTGAAC 
TCCATTATAA AAGCCATGGT TCCCCTCCTT CACATAGCCC TTTTGGTATT ATTTGTAATC 
ATAATCTATG CTATTATAGG ATTGGAACTT TTTATTGGAA AAATGCACAA AACATGTTTT 
TTTGCTGACT CAGATATCGT AGCTGAAGAG GACCCAGCTC CATGTGCGTT CTCAGGGAAT 
GGACGCCAGT GTACTGCCAA TGGCACGGAA TGTAGGAGTG GCTGGGTTGG CCCGAACGGA 
GGCATCACCA ACTTTGATAA CTTTGCCTTT GCCATGCTTA CTGTGTTTCA GTGCATCACC 
ATGGAGGGCT GGACAGATGT GCTCTACTGG GTAAATGATG CGATAGGATG GGAATGGCCA 
TGGGTGTATT TTGTTAGTCT GATCATCCTT GGCTCATTTT TCGTCCTTAA CCTGGTTCTT 
GGTGTCCTTA GTGGAGAATT CTCAAAGGAA AGAGAGAAGG CAAAAGCACG GGGAGATTTC 
CAGAAGCTCC GGGAGAAGCA GCAGCTGGAG GAGGATCTAA AGGGCTACTT GGATTGGATC 
ACCCAAGCTG AGGACATCGA TCCGGAGAAT GAGGAAGAAG GAGGAGAGGA AGGCAAACGA 
AATACTAGCA TGCCCACCAG CGAGACTGAG TCTGTGAACA CAGAGAACGT CAGCGGTGAA 
GGCGAGAACC GAGGCTGCTG TGGAAGTCTC TGGTGCTGGT GGAGACGGAG AGGCGCGGCC 
AAGGCGGGGC CCTCTGGGTG TCGGCGGTGG GGTCAAGCCA TCTCAAAATC CAAACTCAGC 
CGACGCTGGC GTCGCTGGAA CCGATTCAAT CGCAGAAGAT GTAGGGCCGC CGTGAAGTCT 
GTCACGTTTT ACTGGCTGGT TATCGTCCTG GTGTTTCTGA ACACCTTAAC CATTTCCTCT 
GAGCACTACA ATCAGCCAGA TTGGTTGACA CAGATTCAAG ATATTGCCAA CAAAGTCCTC 
TTGGCTCTGT TCACCTGCGA GATGCTGGTA AAAATGTACA GCTTGGGCCT CCAAGCATAT 
TTCGTCTCTC TTTTCAACCG GTTTGATTGC TTCGTGGTGT GTGGTGGAAT CACTGAGACG 
ATCTTGGTGG AACTGGAAAT CATGTCTCCC CTGGGGATCT CTGTGTTTCG GTGTGTGCGC 
CTCTTAAGAA TCTTCAAAGT GACCAGGCAC TGGACTTCCC TGAGCAACTT AGTGGCATCC 
TTATTAAACT CCATGAAGTC CATCGCTTCG CTGTTGCTTC TGCTTTTTCT CTTCATTATC 
ATCTTTTCCT TGCTTGGGAT GCAGCTGTTT GGCGGCAAGT TTAATTTTGA TGAAACGCAA 
ACCAAGCGGA GCACCTTTGA CAATTTCCCT CAAGCACTTC TCACAGTGTT CCAGATCCTG 
ACAGGCGAAG ACTGGAATGC TGTGATGTAC GATGGCATCA TGGCTTACGG GGGCCCATCC 
TCTTCAGGAA TGATCGTCTG CATCTACTTC ATCATCCTCT TCATTTGTGG TAACTATATT 
CTACTGAATG TCTTCTTGGC CATCGCTGTA GACAATTTGG CTGATGCTGA AAGTCTGAAC 
ACTGCTCAGA AAGAAGAAGC GGAAGAAAAG GAGAGGAAAA AGATTGCCAG AAAAGAGAGC 
CTAGAAAATA AAAAGAACAA CAAACCAGAA GTCAACCAGA TAGCCAACAG TGACAACAAG 
GTTACAATTG ATGACTATAG AGAAGAGGAT GAAGACAAGG ACCCCTATCC GCCTTGCGAT 
GTGCCAGTAG GGGAAGAGGA AGAGGAAGAG GAGGAGGATG AACCTGAGGT TCCTGCCGGA 
CCCCGTCCTC GAAGGATCTC GGAGTTGAAC ATGAAGGAAA AAATTGCCCC CATCCCTGAA 
GGGAGCGCTT TCTTCATTCT TAGCAAGACC AACCCGATCC GCGTAGGCTG CCACAAGCTC 
ATCAACCACC ACATCTTCAC CAACCTCATC CTTGTCTTCA TCATGCTGAG CAGCGCTGCC 
CTGGCCGCAG AGGACCCCAT CCGCAGCCAC TCCTTCCGGA ACACGATACT GGGTTACTTT 
GACTATGCCT TCACAGCCAT CTTTACTGTT GAGATCCTGT TGAAGATGAC AACTTTTGGA 
GCTTTCCTCC ACAAAGGGGC CTTCTGCAGG AACTACTTCA ATTTGCTGGA TATGCTGGTG 
GTTGGGGTGT CTCTGGTGTC ATTTGGGATT CAATCCAGTG CCATCTCCGT TGTGAAGATT 
CTGAGGGTCT TAAGGGTCCT GCGTCCCCTC AGGGCCATCA ACAGAGCAAA AGGACTTAAG 
CACGTGGTCC AGTGCGTCTT CGTGGCCATC CGGACCATCG GCAACATCAT GATCGTCACC 
ACCCTCCTGC AGTTCATGTT TGCCTGTATC GGGGTCCAGT TGTTCAAGGG GAAGTTCTAT 
CGCTGTACGG ATGAAGCCAA AAGTAACCCT GAAGAATGCA GGGGACTTTT CATCCTCTAC 
AAGGATGGGG ATGTTGACAG TCCTGTGGTC CGTGAACGGA TCTGGCAAAA CAGTGATTTC 
AACTTCGACA ACGTCCTCTC TGCTATGATG GCGCTCTTCA CAGTCTCCAC GTTTGAGGGC 
TGGCCTGCGT TGCTGTATAA AGCCATCGAC TCGAATGGAG AGAACATCGG CCCAATCTAC 
AACCACCGCG TGGAGATCTC CATCTTCTTC ATCATCTACA TCATCATTGT AGCTTTCTTC 
ATGATGAACA TCTTTGTGGG CTTTGTCATC GTTACATTTC AGGAACAAGG AGAAAAAGAG 
TATAAGAACT GTGAGCTGGA CAAAAATCAG CGTCAGTGTG TTGAATACGC CTTGAAAGCA 
CGTCCCTTGC GGAGATACAT CCCCAAAAAC CCCTACCAGT ACAAGTTCTG GTACGTGGTG 
AACTCTTCGC CTTTCGAATA CATGATGTTT GTCCTCATCA TGCTCAACAC ACTCTGCTTG 
GCCATGCAGC ACTACGAGCA GTCCAAGATG TTCAATGATG CCATGGACAT TCTGAACATG 
GTCTTCACCG GGGTGTTCAC CGTCGAGATG GTTTTGAAAG TCATCGCATT TAAGCCTAAG 
GGGTATTTTA GTGACGCCTG GAACACGTTT GACTCCCTCA TCGTAATCGG CAGCATTATA 
GACGTGGCCC TCAGCGAAGC AGACCACTAT TTCACTGATG CATGGAACAC TTTTGATGCC 
TTAATTGTTG TTGGTAGCGT CGTTGATATT GCTATAACTG AAGTGAATCC AACTGAAAGT 
GAAAATGTCC CTGTCCCAAC TGCTACACCT GGGAACTCTG AAGAGAGCAA TAGAATCTCC 
ATCACCTTTT TCCGTCTTTT CCGAGTGATG CGATTGGTGA AGCTTCTCAG CAGGGGGGAA 
GGCATCCGGA CATTGCTGTG GACTTTTATT AAGTCCTTTC AGGCGCTCCC GTATGTGGCC 
CTCCTCATAG CCATGCTGTT CTTCATCTAT GCGGTCATTG GCATGCAGAT GTTTGGGAAA 
GTTGCCATGA GAGATAACAA CCAGATCAAT AGGAACAATA ACTTCCAGAC GTTTCCCCAG 
GCGGTGCTGC TGCTCTTCAG GTGTGCAACA GGTGAGGCCT GGCAGGAGAT CATGCTGGCC 
TGTCTCCCAG GGAAGCTCTG TGACCCTGAG TCAGATTACA ACCCCGGGGA GGAGTATACA 
TGTGGGAGCA ACTTTGCCAT TGTCTATTTC ATCAGTTTTT ACATGCTCTG TGCATTTCTG 
ATCATCAATC TGTTTGTGGC TGTCATCATG GATAATTTCG ACTATCTGAC CCGGGACTGG 
TCTATTTTGG GGCCTCACCA TTTAGATGAA TTCAAAAGAA TATGGTCAGA ATATGACCCT 
GAGGCAAAGG GAAGGATAAA ACACCTTGAT GTGGTCACTC TGCTTCGACG CATCCAGCCT 
CCCCTGGGGT TTGGGAAGTT ATGTCCACAC AGGGTAGCGT GCAAGAGATT AGTTGCCATG 
AACATGCCTC TCAACAGTGA CGGGACAGTC ATGTTTAATG CAACCCTGTT TGCTTTGGTT 
CGAACGGCTC TTAAGATCAA GACCGAAGGG AACCTGGAGC AAGCTAATGA AGAACTTCGG 
GCTGTGATAA AGAAAATTTG GAAGAAAACC AGCATGAAAT TACTTGACCA AGTTGTCCCT 
CCAGCTGGTG ATGATGAGGT AACCGTGGGG AAGTTCTATG CCACTTTCCT GATACAGGAC 
TACTTTAGGA AATTCAAGAA ACGGAAAGAA CAAGGACTGG TGGGAAAGTA CCCTGCGAAG 
AACACCACAA TTGCCCTACA GGCGGGATTA AGGACACTGC ATGACATTGG GCCAGAAATC 
CGGCGTGCTA TATCGTGTGA TTTGCAAGAT GACGAGCCTG AGGAAACAAA ACGAGAAGAA 
GAAGATGATG TGTTCAAAAG AAATGGTGCC CTGCTTGGAA ACCATGTCAA TCATGTTAAT 
AGTGATAGGA GAGATTCCCT TCAGCAGACC AATACCACCC ACCGTCCCCT GCATGTCCAA 
AGGCCTTCAA TTCCACCTGC AAGTGATACT GAGAAACCGC TGTTTCCTCC AGCAGGAAAT 
TCGGTGTGTC ATAACCATCA TAACCATAAT TCCATAGGAA AGCAAGTTCC CACCTCAACA 
AATGCCAATC TCAATAATGC CAATATGTCC AAAGCTGCCC ATGGAAAGCG GCCCAGCATT 
GGGAACCTTG AGCATGTGTC TGAAAATGGG CATCATTCTT CCCACAAGCA TGACCGGGAG 
CCTCAGAGAA GGTCCAGTGT GAAAAGAACC CGCTATTATG AAACTTACAT TAGGTCCGAC 
TCAGGAGATG AACAGCTCCC AACTATTTGC CGGGAAGACC CAGAGATACA TGGCTATTTC 
AGGGACCCCC ACTGCTTGGG GGAGCAGGAG TATTTCAGTA GTGAGGAATG CTACGAGGAT 
GACAGCTCGC CCACCTGGAG CAGGCAAAAC TATGGCTACT ACAGCAGATA CCCAGGCAGA 
AACATCGACT CTGAGAGGCC CCGAGGCTAC CATCATCCCC AAGGATTCTT GGAGGACGAT 
GACTCGCCCG TTTGCTATGA TTCACGGAGA TCTCCAAGGA GACGCCTACT ACCTCCCACC 
CCAGCATCCC ACCGGAGATC CTCCTTCAAC TTTGAGTGCC TGCGCCGGCA GAGCAGCCAG 
GAAGAGGTCC CGTCGTCTCC CATCTTCCCC CATCGCACGG CCCTGCCTCT GCATCTAATG 
CAGCAACAGA TCATGGCAGT TGCCGGCCTA GATTCAAGTA AAGCCCAGAA GTACTCACCG 
AGTCACTCGA CCCGGTCGTG GGCCACCCCT CCAGCAACCC CTCCCTACCG GGACTGGACA 
CCGTGCTACA CCCCCCTGAT CCAAGTGGAG CAGTCAGAGG CCCTGGACCA GGTGAACGGC 
AGCCTGCCGT CCCTGCACCG CAGCTCCTGG TACACAGACG AGCCCGACAT CTCCTACCGG 
ACTTTCACAC CAGCCAGCCT GACTGTCCCC AGCAGCTTCC GGAACAAAAA CAGCGACAAG 
CAGAGGAGTG CGGACAGCTT GGTGGAGGCA GTCCTGATAT CCGAAGGCTT GGGACGCTAT 
GCAAGGGACC CAAAATTTGT GTCAGCAACA AAACACGAAA TCGCTGATGC CTGTGACCTC 
ACCATCGACG AGATGGAGAG TGCAGCCAGC ACCCTGCTTA ATGGGAACGT GCGTCCCCGA 
GCCAACGGGG ATGTGGGCCC CCTCTCACAC CGGCAGGACT ATGAGCTACA GGACTTTGGT 
CCTGGCTACA GCGACGAAGA GCCAGACCCT GGGAGGGATG AGGAGGACCT GGCGGATGAA 
ATGATATGCA TCACCACCTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1D ( XM_011534100.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011532402.1 Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X15, mRNA.

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CloneID	OHu56581
Clone ID Related Accession (Same CDS sequence)	XM_011534100.2
Accession Version	XM_011534100.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	voltage-dependent L-type calcium channel subunit alpha-1D isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011534100.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGGGG AGCCGCTGCC GCTGTGGGGC TCCCCTCCCC AGCTCTGGCC CGGGCACCAC 
GTGCAGCTTC CACGCCGGCC GGGGCTGGGT CGAGGGAGGA TGGAGCAGCT CCTGGCCAGC 
CCTCCCCTTT GGAGACCGCC AGTGCCCCCC AACCCCTGTC GGCTGGAAGC CTTTCCGGAG 
GCAAACTATG CAAGAGGCAC CAGACTTCCT CTTTCTGGTG AAGGACCAAC TTCTCAGCCG 
AATAGCTCCA AGCAAACTGT CCTGTCTTGG CAAGCTGCAA TCGATGCTGC TAGACAGGCC 
AAGGCTGCCC AAACTATGAG CACCTCTGCA CCCCCACCTG TAGGATCTCT CTCCCAAAGA 
AAACGTCAGC AATACGCCAA GAGCAAAAAA CAGGGTAACT CGTCCAACAG CCGACCTGCC 
CGCGCCCTTT TCTGTTTATC ACTCAATAAC CCCATCCGAA GAGCCTGCAT TAGTATAGTG 
GAATGGAAAC CATTTGACAT ATTTATATTA TTGGCTATTT TTGCCAATTG TGTGGCCTTA 
GCTATTTACA TCCCATTCCC TGAAGATGAT TCTAATTCAA CAAATCATAA CTTGGAAAAA 
GTAGAATATG CCTTCCTGAT TATTTTTACA GTCGAGACAT TTTTGAAGAT TATAGCGTAT 
GGATTATTGC TACATCCTAA TGCTTATGTT AGGAATGGAT GGAATTTACT GGATTTTGTT 
ATAGTAATAG TAGGATTGTT TAGTGTAATT TTGGAACAAT TAACCAAAGA AACAGAAGGC 
GGGAACCACT CAAGCGGCAA ATCTGGAGGC TTTGATGTCA AAGCCCTCCG TGCCTTTCGA 
GTGTTGCGAC CACTTCGACT AGTGTCAGGA GTGCCCAGTT TACAAGTTGT CCTGAACTCC 
ATTATAAAAG CCATGGTTCC CCTCCTTCAC ATAGCCCTTT TGGTATTATT TGTAATCATA 
ATCTATGCTA TTATAGGATT GGAACTTTTT ATTGGAAAAA TGCACAAAAC ATGTTTTTTT 
GCTGACTCAG ATATCGTAGC TGAAGAGGAC CCAGCTCCAT GTGCGTTCTC AGGGAATGGA 
CGCCAGTGTA CTGCCAATGG CACGGAATGT AGGAGTGGCT GGGTTGGCCC GAACGGAGGC 
ATCACCAACT TTGATAACTT TGCCTTTGCC ATGCTTACTG TGTTTCAGTG CATCACCATG 
GAGGGCTGGA CAGATGTGCT CTACTGGGTA AATGATGCGA TAGGATGGGA ATGGCCATGG 
GTGTATTTTG TTAGTCTGAT CATCCTTGGC TCATTTTTCG TCCTTAACCT GGTTCTTGGT 
GTCCTTAGTG GAGAATTCTC AAAGGAAAGA GAGAAGGCAA AAGCACGGGG AGATTTCCAG 
AAGCTCCGGG AGAAGCAGCA GCTGGAGGAG GATCTAAAGG GCTACTTGGA TTGGATCACC 
CAAGCTGAGG ACATCGATCC GGAGAATGAG GAAGAAGGAG GAGAGGAAGG CAAACGAAAT 
ACTAGCATGC CCACCAGCGA GACTGAGTCT GTGAACACAG AGAACGTCAG CGGTGAAGGC 
GAGAACCGAG GCTGCTGTGG AAGTCTCTGT CAAGCCATCT CAAAATCCAA ACTCAGCCGA 
CGCTGGCGTC GCTGGAACCG ATTCAATCGC AGAAGATGTA GGGCCGCCGT GAAGTCTGTC 
ACGTTTTACT GGCTGGTTAT CGTCCTGGTG TTTCTGAACA CCTTAACCAT TTCCTCTGAG 
CACTACAATC AGCCAGATTG GTTGACACAG ATTCAAGATA TTGCCAACAA AGTCCTCTTG 
GCTCTGTTCA CCTGCGAGAT GCTGGTAAAA ATGTACAGCT TGGGCCTCCA AGCATATTTC 
GTCTCTCTTT TCAACCGGTT TGATTGCTTC GTGGTGTGTG GTGGAATCAC TGAGACGATC 
TTGGTGGAAC TGGAAATCAT GTCTCCCCTG GGGATCTCTG TGTTTCGGTG TGTGCGCCTC 
TTAAGAATCT TCAAAGTGAC CAGGCACTGG ACTTCCCTGA GCAACTTAGT GGCATCCTTA 
TTAAACTCCA TGAAGTCCAT CGCTTCGCTG TTGCTTCTGC TTTTTCTCTT CATTATCATC 
TTTTCCTTGC TTGGGATGCA GCTGTTTGGC GGCAAGTTTA ATTTTGATGA AACGCAAACC 
AAGCGGAGCA CCTTTGACAA TTTCCCTCAA GCACTTCTCA CAGTGTTCCA GATCCTGACA 
GGCGAAGACT GGAATGCTGT GATGTACGAT GGCATCATGG CTTACGGGGG CCCATCCTCT 
TCAGGAATGA TCGTCTGCAT CTACTTCATC ATCCTCTTCA TTTGTGGTAA CTATATTCTA 
CTGAATGTCT TCTTGGCCAT CGCTGTAGAC AATTTGGCTG ATGCTGAAAG TCTGAACACT 
GCTCAGAAAG AAGAAGCGGA AGAAAAGGAG AGGAAAAAGA TTGCCAGAAA AGAGAGCCTA 
GAAAATAAAA AGAACAACAA ACCAGAAGTC AACCAGATAG CCAACAGTGA CAACAAGGTT 
ACAATTGATG ACTATAGAGA AGAGGATGAA GACAAGGACC CCTATCCGCC TTGCGATGTG 
CCAGTAGGGG AAGAGGAAGA GGAAGAGGAG GAGGATGAAC CTGAGGTTCC TGCCGGACCC 
CGTCCTCGAA GGATCTCGGA GTTGAACATG AAGGAAAAAA TTGCCCCCAT CCCTGAAGGG 
AGCGCTTTCT TCATTCTTAG CAAGACCAAC CCGATCCGCG TAGGCTGCCA CAAGCTCATC 
AACCACCACA TCTTCACCAA CCTCATCCTT GTCTTCATCA TGCTGAGCAG CGCTGCCCTG 
GCCGCAGAGG ACCCCATCCG CAGCCACTCC TTCCGGAACA CGATACTGGG TTACTTTGAC 
TATGCCTTCA CAGCCATCTT TACTGTTGAG ATCCTGTTGA AGATGACAAC TTTTGGAGCT 
TTCCTCCACA AAGGGGCCTT CTGCAGGAAC TACTTCAATT TGCTGGATAT GCTGGTGGTT 
GGGGTGTCTC TGGTGTCATT TGGGATTCAA TCCAGTGCCA TCTCCGTTGT GAAGATTCTG 
AGGGTCTTAA GGGTCCTGCG TCCCCTCAGG GCCATCAACA GAGCAAAAGG ACTTAAGCAC 
GTGGTCCAGT GCGTCTTCGT GGCCATCCGG ACCATCGGCA ACATCATGAT CGTCACCACC 
CTCCTGCAGT TCATGTTTGC CTGTATCGGG GTCCAGTTGT TCAAGGGGAA GTTCTATCGC 
TGTACGGATG AAGCCAAAAG TAACCCTGAA GAATGCAGGG GACTTTTCAT CCTCTACAAG 
GATGGGGATG TTGACAGTCC TGTGGTCCGT GAACGGATCT GGCAAAACAG TGATTTCAAC 
TTCGACAACG TCCTCTCTGC TATGATGGCG CTCTTCACAG TCTCCACGTT TGAGGGCTGG 
CCTGCGTTGC TGTATAAAGC CATCGACTCG AATGGAGAGA ACATCGGCCC AATCTACAAC 
CACCGCGTGG AGATCTCCAT CTTCTTCATC ATCTACATCA TCATTGTAGC TTTCTTCATG 
ATGAACATCT TTGTGGGCTT TGTCATCGTT ACATTTCAGG AACAAGGAGA AAAAGAGTAT 
AAGAACTGTG AGCTGGACAA AAATCAGCGT CAGTGTGTTG AATACGCCTT GAAAGCACGT 
CCCTTGCGGA GATACATCCC CAAAAACCCC TACCAGTACA AGTTCTGGTA CGTGGTGAAC 
TCTTCGCCTT TCGAATACAT GATGTTTGTC CTCATCATGC TCAACACACT CTGCTTGGCC 
ATGCAGCACT ACGAGCAGTC CAAGATGTTC AATGATGCCA TGGACATTCT GAACATGGTC 
TTCACCGGGG TGTTCACCGT CGAGATGGTT TTGAAAGTCA TCGCATTTAA GCCTAAGGGG 
TATTTTAGTG ACGCCTGGAA CACGTTTGAC TCCCTCATCG TAATCGGCAG CATTATAGAC 
GTGGCCCTCA GCGAAGCAGA CCACTATTTC ACTGATGCAT GGAACACTTT TGATGCCTTA 
ATTGTTGTTG GTAGCGTCGT TGATATTGCT ATAACTGAAG TGAATAACTC TGAAGAGAGC 
AATAGAATCT CCATCACCTT TTTCCGTCTT TTCCGAGTGA TGCGATTGGT GAAGCTTCTC 
AGCAGGGGGG AAGGCATCCG GACATTGCTG TGGACTTTTA TTAAGTCCTT TCAGGCGCTC 
CCGTATGTGG CCCTCCTCAT AGCCATGCTG TTCTTCATCT ATGCGGTCAT TGGCATGCAG 
ATGTTTGGGA AAGTTGCCAT GAGAGATAAC AACCAGATCA ATAGGAACAA TAACTTCCAG 
ACGTTTCCCC AGGCGGTGCT GCTGCTCTTC AGGTGTGCAA CAGGTGAGGC CTGGCAGGAG 
ATCATGCTGG CCTGTCTCCC AGGGAAGCTC TGTGACCCTG AGTCAGATTA CAACCCCGGG 
GAGGAGTATA CATGTGGGAG CAACTTTGCC ATTGTCTATT TCATCAGTTT TTACATGCTC 
TGTGCATTTC TGATCATCAA TCTGTTTGTG GCTGTCATCA TGGATAATTT CGACTATCTG 
ACCCGGGACT GGTCTATTTT GGGGCCTCAC CATTTAGATG AATTCAAAAG AATATGGTCA 
GAATATGACC CTGAGGCAAA GGGAAGGATA AAACACCTTG ATGTGGTCAC TCTGCTTCGA 
CGCATCCAGC CTCCCCTGGG GTTTGGGAAG TTATGTCCAC ACAGGGTAGC GTGCAAGAGA 
TTAGTTGCCA TGAACATGCC TCTCAACAGT GACGGGACAG TCATGTTTAA TGCAACCCTG 
TTTGCTTTGG TTCGAACGGC TCTTAAGATC AAGACCGAAG GGAACCTGGA GCAAGCTAAT 
GAAGAACTTC GGGCTGTGAT AAAGAAAATT TGGAAGAAAA CCAGCATGAA ATTACTTGAC 
CAAGTTGTCC CTCCAGCTGG TGATGATGAG GTAACCGTGG GGAAGTTCTA TGCCACTTTC 
CTGATACAGG ACTACTTTAG GAAATTCAAG AAACGGAAAG AACAAGGACT GGTGGGAAAG 
TACCCTGCGA AGAACACCAC AATTGCCCTA CAGGCGGGAT TAAGGACACT GCATGACATT 
GGGCCAGAAA TCCGGCGTGC TATATCGTGT GATTTGCAAG ATGACGAGCC TGAGGAAACA 
AAACGAGAAG AAGAAGATGA TGTGTTCAAA AGAAATGGTG CCCTGCTTGG AAACCATGTC 
AATCATGTTA ATAGTGATAG GAGAGATTCC CTTCAGCAGA CCAATACCAC CCACCGTCCC 
CTGCATGTCC AAAGGCCTTC AATTCCACCT GCAAGTGATA CTGAGAAACC GCTGTTTCCT 
CCAGCAGGAA ATTCGGTGTG TCATAACCAT CATAACCATA ATTCCATAGG AAAGCAAGTT 
CCCACCTCAA CAAATGCCAA TCTCAATAAT GCCAATATGT CCAAAGCTGC CCATGGAAAG 
CGGCCCAGCA TTGGGAACCT TGAGCATGTG TCTGAAAATG GGCATCATTC TTCCCACAAG 
CATGACCGGG AGCCTCAGAG AAGGTCCAGT GTGAAAAGAA CCCGCTATTA TGAAACTTAC 
ATTAGGTCCG ACTCAGGAGA TGAACAGCTC CCAACTATTT GCCGGGAAGA CCCAGAGATA 
CATGGCTATT TCAGGGACCC CCACTGCTTG GGGGAGCAGG AGTATTTCAG TAGTGAGGAA 
TGCTACGAGG ATGACAGCTC GCCCACCTGG AGCAGGCAAA ACTATGGCTA CTACAGCAGA 
TACCCAGGCA GAAACATCGA CTCTGAGAGG CCCCGAGGCT ACCATCATCC CCAAGGATTC 
TTGGAGGACG ATGACTCGCC CGTTTGCTAT GATTCACGGA GATCTCCAAG GAGACGCCTA 
CTACCTCCCA CCCCAGCATC CCACCGGAGA TCCTCCTTCA ACTTTGAGTG CCTGCGCCGG 
CAGAGCAGCC AGGAAGAGGT CCCGTCGTCT CCCATCTTCC CCCATCGCAC GGCCCTGCCT 
CTGCATCTAA TGCAGCAACA GATCATGGCA GTTGCCGGCC TAGATTCAAG TAAAGCCCAG 
AAGTACTCAC CGAGTCACTC GACCCGGTCG TGGGCCACCC CTCCAGCAAC CCCTCCCTAC 
CGGGACTGGA CACCGTGCTA CACCCCCCTG ATCCAAGTGG AGCAGTCAGA GGCCCTGGAC 
CAGGTGAACG GCAGCCTGCC GTCCCTGCAC CGCAGCTCCT GGTACACAGA CGAGCCCGAC 
ATCTCCTACC GGACTTTCAC ACCAGCCAGC CTGACTGTCC CCAGCAGCTT CCGGAACAAA 
AACAGCGACA AGCAGAGGAG TGCGGACAGC TTGGTGGAGG CAGTCCTGAT ATCCGAAGGC 
TTGGGACGCT ATGCAAGGGA CCCAAAATTT GTGTCAGCAA CAAAACACGA AATCGCTGAT 
GCCTGTGACC TCACCATCGA CGAGATGGAG AGTGCAGCCA GCACCCTGCT TAATGGGAAC 
GTGCGTCCCC GAGCCAACGG GGATGTGGGC CCCCTCTCAC ACCGGCAGGA CTATGAGCTA 
CAGGACTTTG GTCCTGGCTA CAGCGACGAA GAGCCAGACC CTGGGAGGGA TGAGGAGGAC 
CTGGCGGATG AAATGATATG CATCACCACC TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011532402.1
CDS	16..6651
Translation	MPGEPLPLWGSPPQLWPGHHVQLPRRPGLGRGRMEQLLASPPLWRPPVPPNPCRLEAFPEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMSTSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANCVALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLFSVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGENRGCCGSLCQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTISSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIMSPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDETQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAIAVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPPCDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTNLILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDMLVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDSNGENIGPIYNHRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDKNQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDILNMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADHYFTDAWNTFDALIVVGSVVDIAITEVNNSEESNRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDNNQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLDQVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISCDLQDDEPEETKREEEDDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFPPAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGNLEHVSENGHHSSHKHDREPQRRSSVKRTRYYETYIRSDSGDEQLPTICREDPEIHGYFRDPHCLGEQEYFSSEECYEDDSSPTWSRQNYGYYSRYPGRNIDSERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPASHRRSSFNFECLRRQSSQEEVPSSPIFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVEQSEALDQVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKFVSATKHEIADACDLTIDEMESAASTLLNGNVRPRANGDVGPLSHRQDYELQDFGPGYSDEEPDPGRDEEDLADEMICITTL

Target ORF information:

RefSeq Version	XM_011534100.2
Organism	Homo sapiens(human)
Definition	Homo sapiens calcium voltage-gated channel subunit alpha1 D (CACNA1D), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011534100.2

ORF Insert Sequence:

ATGCCCGGGG AGCCGCTGCC GCTGTGGGGC TCCCCTCCCC AGCTCTGGCC CGGGCACCAC 
GTGCAGCTTC CACGCCGGCC GGGGCTGGGT CGAGGGAGGA TGGAGCAGCT CCTGGCCAGC 
CCTCCCCTTT GGAGACCGCC AGTGCCCCCC AACCCCTGTC GGCTGGAAGC CTTTCCGGAG 
GCAAACTATG CAAGAGGCAC CAGACTTCCT CTTTCTGGTG AAGGACCAAC TTCTCAGCCG 
AATAGCTCCA AGCAAACTGT CCTGTCTTGG CAAGCTGCAA TCGATGCTGC TAGACAGGCC 
AAGGCTGCCC AAACTATGAG CACCTCTGCA CCCCCACCTG TAGGATCTCT CTCCCAAAGA 
AAACGTCAGC AATACGCCAA GAGCAAAAAA CAGGGTAACT CGTCCAACAG CCGACCTGCC 
CGCGCCCTTT TCTGTTTATC ACTCAATAAC CCCATCCGAA GAGCCTGCAT TAGTATAGTG 
GAATGGAAAC CATTTGACAT ATTTATATTA TTGGCTATTT TTGCCAATTG TGTGGCCTTA 
GCTATTTACA TCCCATTCCC TGAAGATGAT TCTAATTCAA CAAATCATAA CTTGGAAAAA 
GTAGAATATG CCTTCCTGAT TATTTTTACA GTCGAGACAT TTTTGAAGAT TATAGCGTAT 
GGATTATTGC TACATCCTAA TGCTTATGTT AGGAATGGAT GGAATTTACT GGATTTTGTT 
ATAGTAATAG TAGGATTGTT TAGTGTAATT TTGGAACAAT TAACCAAAGA AACAGAAGGC 
GGGAACCACT CAAGCGGCAA ATCTGGAGGC TTTGATGTCA AAGCCCTCCG TGCCTTTCGA 
GTGTTGCGAC CACTTCGACT AGTGTCAGGA GTGCCCAGTT TACAAGTTGT CCTGAACTCC 
ATTATAAAAG CCATGGTTCC CCTCCTTCAC ATAGCCCTTT TGGTATTATT TGTAATCATA 
ATCTATGCTA TTATAGGATT GGAACTTTTT ATTGGAAAAA TGCACAAAAC ATGTTTTTTT 
GCTGACTCAG ATATCGTAGC TGAAGAGGAC CCAGCTCCAT GTGCGTTCTC AGGGAATGGA 
CGCCAGTGTA CTGCCAATGG CACGGAATGT AGGAGTGGCT GGGTTGGCCC GAACGGAGGC 
ATCACCAACT TTGATAACTT TGCCTTTGCC ATGCTTACTG TGTTTCAGTG CATCACCATG 
GAGGGCTGGA CAGATGTGCT CTACTGGGTA AATGATGCGA TAGGATGGGA ATGGCCATGG 
GTGTATTTTG TTAGTCTGAT CATCCTTGGC TCATTTTTCG TCCTTAACCT GGTTCTTGGT 
GTCCTTAGTG GAGAATTCTC AAAGGAAAGA GAGAAGGCAA AAGCACGGGG AGATTTCCAG 
AAGCTCCGGG AGAAGCAGCA GCTGGAGGAG GATCTAAAGG GCTACTTGGA TTGGATCACC 
CAAGCTGAGG ACATCGATCC GGAGAATGAG GAAGAAGGAG GAGAGGAAGG CAAACGAAAT 
ACTAGCATGC CCACCAGCGA GACTGAGTCT GTGAACACAG AGAACGTCAG CGGTGAAGGC 
GAGAACCGAG GCTGCTGTGG AAGTCTCTGT CAAGCCATCT CAAAATCCAA ACTCAGCCGA 
CGCTGGCGTC GCTGGAACCG ATTCAATCGC AGAAGATGTA GGGCCGCCGT GAAGTCTGTC 
ACGTTTTACT GGCTGGTTAT CGTCCTGGTG TTTCTGAACA CCTTAACCAT TTCCTCTGAG 
CACTACAATC AGCCAGATTG GTTGACACAG ATTCAAGATA TTGCCAACAA AGTCCTCTTG 
GCTCTGTTCA CCTGCGAGAT GCTGGTAAAA ATGTACAGCT TGGGCCTCCA AGCATATTTC 
GTCTCTCTTT TCAACCGGTT TGATTGCTTC GTGGTGTGTG GTGGAATCAC TGAGACGATC 
TTGGTGGAAC TGGAAATCAT GTCTCCCCTG GGGATCTCTG TGTTTCGGTG TGTGCGCCTC 
TTAAGAATCT TCAAAGTGAC CAGGCACTGG ACTTCCCTGA GCAACTTAGT GGCATCCTTA 
TTAAACTCCA TGAAGTCCAT CGCTTCGCTG TTGCTTCTGC TTTTTCTCTT CATTATCATC 
TTTTCCTTGC TTGGGATGCA GCTGTTTGGC GGCAAGTTTA ATTTTGATGA AACGCAAACC 
AAGCGGAGCA CCTTTGACAA TTTCCCTCAA GCACTTCTCA CAGTGTTCCA GATCCTGACA 
GGCGAAGACT GGAATGCTGT GATGTACGAT GGCATCATGG CTTACGGGGG CCCATCCTCT 
TCAGGAATGA TCGTCTGCAT CTACTTCATC ATCCTCTTCA TTTGTGGTAA CTATATTCTA 
CTGAATGTCT TCTTGGCCAT CGCTGTAGAC AATTTGGCTG ATGCTGAAAG TCTGAACACT 
GCTCAGAAAG AAGAAGCGGA AGAAAAGGAG AGGAAAAAGA TTGCCAGAAA AGAGAGCCTA 
GAAAATAAAA AGAACAACAA ACCAGAAGTC AACCAGATAG CCAACAGTGA CAACAAGGTT 
ACAATTGATG ACTATAGAGA AGAGGATGAA GACAAGGACC CCTATCCGCC TTGCGATGTG 
CCAGTAGGGG AAGAGGAAGA GGAAGAGGAG GAGGATGAAC CTGAGGTTCC TGCCGGACCC 
CGTCCTCGAA GGATCTCGGA GTTGAACATG AAGGAAAAAA TTGCCCCCAT CCCTGAAGGG 
AGCGCTTTCT TCATTCTTAG CAAGACCAAC CCGATCCGCG TAGGCTGCCA CAAGCTCATC 
AACCACCACA TCTTCACCAA CCTCATCCTT GTCTTCATCA TGCTGAGCAG CGCTGCCCTG 
GCCGCAGAGG ACCCCATCCG CAGCCACTCC TTCCGGAACA CGATACTGGG TTACTTTGAC 
TATGCCTTCA CAGCCATCTT TACTGTTGAG ATCCTGTTGA AGATGACAAC TTTTGGAGCT 
TTCCTCCACA AAGGGGCCTT CTGCAGGAAC TACTTCAATT TGCTGGATAT GCTGGTGGTT 
GGGGTGTCTC TGGTGTCATT TGGGATTCAA TCCAGTGCCA TCTCCGTTGT GAAGATTCTG 
AGGGTCTTAA GGGTCCTGCG TCCCCTCAGG GCCATCAACA GAGCAAAAGG ACTTAAGCAC 
GTGGTCCAGT GCGTCTTCGT GGCCATCCGG ACCATCGGCA ACATCATGAT CGTCACCACC 
CTCCTGCAGT TCATGTTTGC CTGTATCGGG GTCCAGTTGT TCAAGGGGAA GTTCTATCGC 
TGTACGGATG AAGCCAAAAG TAACCCTGAA GAATGCAGGG GACTTTTCAT CCTCTACAAG 
GATGGGGATG TTGACAGTCC TGTGGTCCGT GAACGGATCT GGCAAAACAG TGATTTCAAC 
TTCGACAACG TCCTCTCTGC TATGATGGCG CTCTTCACAG TCTCCACGTT TGAGGGCTGG 
CCTGCGTTGC TGTATAAAGC CATCGACTCG AATGGAGAGA ACATCGGCCC AATCTACAAC 
CACCGCGTGG AGATCTCCAT CTTCTTCATC ATCTACATCA TCATTGTAGC TTTCTTCATG 
ATGAACATCT TTGTGGGCTT TGTCATCGTT ACATTTCAGG AACAAGGAGA AAAAGAGTAT 
AAGAACTGTG AGCTGGACAA AAATCAGCGT CAGTGTGTTG AATACGCCTT GAAAGCACGT 
CCCTTGCGGA GATACATCCC CAAAAACCCC TACCAGTACA AGTTCTGGTA CGTGGTGAAC 
TCTTCGCCTT TCGAATACAT GATGTTTGTC CTCATCATGC TCAACACACT CTGCTTGGCC 
ATGCAGCACT ACGAGCAGTC CAAGATGTTC AATGATGCCA TGGACATTCT GAACATGGTC 
TTCACCGGGG TGTTCACCGT CGAGATGGTT TTGAAAGTCA TCGCATTTAA GCCTAAGGGG 
TATTTTAGTG ACGCCTGGAA CACGTTTGAC TCCCTCATCG TAATCGGCAG CATTATAGAC 
GTGGCCCTCA GCGAAGCAGA CCACTATTTC ACTGATGCAT GGAACACTTT TGATGCCTTA 
ATTGTTGTTG GTAGCGTCGT TGATATTGCT ATAACTGAAG TGAATAACTC TGAAGAGAGC 
AATAGAATCT CCATCACCTT TTTCCGTCTT TTCCGAGTGA TGCGATTGGT GAAGCTTCTC 
AGCAGGGGGG AAGGCATCCG GACATTGCTG TGGACTTTTA TTAAGTCCTT TCAGGCGCTC 
CCGTATGTGG CCCTCCTCAT AGCCATGCTG TTCTTCATCT ATGCGGTCAT TGGCATGCAG 
ATGTTTGGGA AAGTTGCCAT GAGAGATAAC AACCAGATCA ATAGGAACAA TAACTTCCAG 
ACGTTTCCCC AGGCGGTGCT GCTGCTCTTC AGGTGTGCAA CAGGTGAGGC CTGGCAGGAG 
ATCATGCTGG CCTGTCTCCC AGGGAAGCTC TGTGACCCTG AGTCAGATTA CAACCCCGGG 
GAGGAGTATA CATGTGGGAG CAACTTTGCC ATTGTCTATT TCATCAGTTT TTACATGCTC 
TGTGCATTTC TGATCATCAA TCTGTTTGTG GCTGTCATCA TGGATAATTT CGACTATCTG 
ACCCGGGACT GGTCTATTTT GGGGCCTCAC CATTTAGATG AATTCAAAAG AATATGGTCA 
GAATATGACC CTGAGGCAAA GGGAAGGATA AAACACCTTG ATGTGGTCAC TCTGCTTCGA 
CGCATCCAGC CTCCCCTGGG GTTTGGGAAG TTATGTCCAC ACAGGGTAGC GTGCAAGAGA 
TTAGTTGCCA TGAACATGCC TCTCAACAGT GACGGGACAG TCATGTTTAA TGCAACCCTG 
TTTGCTTTGG TTCGAACGGC TCTTAAGATC AAGACCGAAG GGAACCTGGA GCAAGCTAAT 
GAAGAACTTC GGGCTGTGAT AAAGAAAATT TGGAAGAAAA CCAGCATGAA ATTACTTGAC 
CAAGTTGTCC CTCCAGCTGG TGATGATGAG GTAACCGTGG GGAAGTTCTA TGCCACTTTC 
CTGATACAGG ACTACTTTAG GAAATTCAAG AAACGGAAAG AACAAGGACT GGTGGGAAAG 
TACCCTGCGA AGAACACCAC AATTGCCCTA CAGGCGGGAT TAAGGACACT GCATGACATT 
GGGCCAGAAA TCCGGCGTGC TATATCGTGT GATTTGCAAG ATGACGAGCC TGAGGAAACA 
AAACGAGAAG AAGAAGATGA TGTGTTCAAA AGAAATGGTG CCCTGCTTGG AAACCATGTC 
AATCATGTTA ATAGTGATAG GAGAGATTCC CTTCAGCAGA CCAATACCAC CCACCGTCCC 
CTGCATGTCC AAAGGCCTTC AATTCCACCT GCAAGTGATA CTGAGAAACC GCTGTTTCCT 
CCAGCAGGAA ATTCGGTGTG TCATAACCAT CATAACCATA ATTCCATAGG AAAGCAAGTT 
CCCACCTCAA CAAATGCCAA TCTCAATAAT GCCAATATGT CCAAAGCTGC CCATGGAAAG 
CGGCCCAGCA TTGGGAACCT TGAGCATGTG TCTGAAAATG GGCATCATTC TTCCCACAAG 
CATGACCGGG AGCCTCAGAG AAGGTCCAGT GTGAAAAGAA CCCGCTATTA TGAAACTTAC 
ATTAGGTCCG ACTCAGGAGA TGAACAGCTC CCAACTATTT GCCGGGAAGA CCCAGAGATA 
CATGGCTATT TCAGGGACCC CCACTGCTTG GGGGAGCAGG AGTATTTCAG TAGTGAGGAA 
TGCTACGAGG ATGACAGCTC GCCCACCTGG AGCAGGCAAA ACTATGGCTA CTACAGCAGA 
TACCCAGGCA GAAACATCGA CTCTGAGAGG CCCCGAGGCT ACCATCATCC CCAAGGATTC 
TTGGAGGACG ATGACTCGCC CGTTTGCTAT GATTCACGGA GATCTCCAAG GAGACGCCTA 
CTACCTCCCA CCCCAGCATC CCACCGGAGA TCCTCCTTCA ACTTTGAGTG CCTGCGCCGG 
CAGAGCAGCC AGGAAGAGGT CCCGTCGTCT CCCATCTTCC CCCATCGCAC GGCCCTGCCT 
CTGCATCTAA TGCAGCAACA GATCATGGCA GTTGCCGGCC TAGATTCAAG TAAAGCCCAG 
AAGTACTCAC CGAGTCACTC GACCCGGTCG TGGGCCACCC CTCCAGCAAC CCCTCCCTAC 
CGGGACTGGA CACCGTGCTA CACCCCCCTG ATCCAAGTGG AGCAGTCAGA GGCCCTGGAC 
CAGGTGAACG GCAGCCTGCC GTCCCTGCAC CGCAGCTCCT GGTACACAGA CGAGCCCGAC 
ATCTCCTACC GGACTTTCAC ACCAGCCAGC CTGACTGTCC CCAGCAGCTT CCGGAACAAA 
AACAGCGACA AGCAGAGGAG TGCGGACAGC TTGGTGGAGG CAGTCCTGAT ATCCGAAGGC 
TTGGGACGCT ATGCAAGGGA CCCAAAATTT GTGTCAGCAA CAAAACACGA AATCGCTGAT 
GCCTGTGACC TCACCATCGA CGAGATGGAG AGTGCAGCCA GCACCCTGCT TAATGGGAAC 
GTGCGTCCCC GAGCCAACGG GGATGTGGGC CCCCTCTCAC ACCGGCAGGA CTATGAGCTA 
CAGGACTTTG GTCCTGGCTA CAGCGACGAA GAGCCAGACC CTGGGAGGGA TGAGGAGGAC 
CTGGCGGATG AAATGATATG CATCACCACC TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	CACNA1D
Entrez Gene ID	776
Full Name	calcium voltage-gated channel subunit alpha1 D
Synonyms	CACH3,CACN4,CACNL1A2,CCHL1A2,Cav1.3,PASNA,SANDD
General protein information	Preferred Names calcium voltage-gated channel subunit alpha1 D Names voltage-dependent L-type calcium channel subunit alpha-1D calcium channel, L type, alpha-1 polypeptide calcium channel, neuroendocrine/brain-type, alpha 1 subunit calcium channel, voltage-dependent, L type, alpha 1D subunit voltage-gated calcium channel alpha 1 subunit voltage-gated calcium channel alpha subunit Cav1.3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p21.1
Summary	Voltage-dependent calcium channels mediate the entry of calcium ions into excitable cells, and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, and gene expression. Calcium channels are multisubunit complexes composed of alpha-1, beta, alpha-2/delta, and gamma subunits. The channel activity is directed by the pore-forming alpha-1 subunit, whereas the others act as auxiliary subunits regulating this activity. The distinctive properties of the calcium channel types are related primarily to the expression of a variety of alpha-1 isoforms, namely alpha-1A, B, C, D, E, and S. This gene encodes the alpha-1D subunit. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012].
Disorder MIM:	114206
Disorder Html:	Sinoatrial node dysfunction and deafness, 614896 (3); Primary aldosteronism, seizures, and neurologic abnormalities, 615474 (3)

Bos taurus (cattle)	CACNA1D	NP_001179954.1
Rattus norvegicus (Norway rat)	Cacna1d	NP_058994.1
Gallus gallus (chicken)	CACNA1D	NP_990365.1
Homo sapiens (human)	CACNA1D	NP_000711.1
Pan troglodytes (chimpanzee)	CACNA1D	XP_001173076.3
	CACNA1D	XP_002802833.1
Mus musculus (house mouse)	Cacna1d	NP_001077085.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012434	XP_001231059.2
Canis lupus familiaris (dog)	CACNA1D	XP_849431.1
Danio rerio (zebrafish)	cacna1da	NP_982351.1
Drosophila melanogaster (fruit fly)	Ca-alpha1D	NP_001260484.1
Caenorhabditis elegans (roundworm)	egl-19	NP_001023079.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

CACNA1D cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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