BPHL cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	BPHL
Entrez Gene ID	670
Full Name	biphenyl hydrolase like
Synonyms	BPH-RP,MCNAA,VACVASE
General protein information	Preferred Names biphenyl hydrolase like Names valacyclovir hydrolase biphenyl hydrolase-like (serine hydrolase) biphenyl hydrolase-related protein breast epithelial mucin-associated antigen valacyclovirase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p25.2
Summary	This gene encodes a member of the serine protease family of hydrolytic enzymes which contain a serine in their active site. The encoded protein may play a role in activation of the antiviral prodrug valacyclovir. Alternatively spliced transcript variants have been described.[provided by RefSeq, Jan 2009].
Disorder MIM:	603156

mRNA and Protein(s)

mRNA	Protein	Name
NM_001302777.1	NP_001289706.1	valacyclovir hydrolase isoform 2
NM_004332.3	NP_004323.2	valacyclovir hydrolase isoform 1 precursor
NM_004332.4	NP_004323.2	valacyclovir hydrolase isoform 1 precursor

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	BPHL	XP_004939728.1
Caenorhabditis elegans (roundworm)	K01A2.5	NP_493701.1
	BPHL	XP_001092229.2
Canis lupus familiaris (dog)	BPHL	XP_535867.3
Bos taurus (cattle)	BPHL	NP_001039383.1
Rattus norvegicus (Norway rat)	Bphl	NP_001032283.1
Pan troglodytes (chimpanzee)	BPHL	XP_001161505.1
Danio rerio (zebrafish)	LOC402804	XP_005174600.1
Drosophila melanogaster (fruit fly)	CG5377	NP_651052.1
Mus musculus (house mouse)	Bphl	NP_080788.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003440	XP_311731.4
Homo sapiens (human)	BPHL	NP_004323.2
Xenopus tropicalis (tropical clawed frog)	bphl	XP_002936463.1

Gene Ontology
Bibliography

Related articles in PubMed

Human valacyclovir hydrolase/biphenyl hydrolase-like protein is a highly efficient homocysteine thiolactonase.
Marsillach J, Suzuki SM, Richter RJ, McDonald MG, Rademacher PM, MacCoss MJ, Hsieh EJ, Rettie AE, Furlong CE
PloS one9(10)e110054(2014)

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
Hendrickson SL, Lautenberger JA, Chinn LW, Malasky M, Sezgin E, Kingsley LA, Goedert JJ, Kirk GD, Gomperts ED, Buchbinder SP, Troyer JL, O'Brien SJ
PloS one5(9)e12862(2010 Sep)

New genetic associations detected in a host response study to hepatitis B vaccine.
Davila S, Froeling FE, Tan A, Bonnard C, Boland GJ, Snippe H, Hibberd ML, Seielstad M
Genes and immunity11(3)232-8(2010 Apr)

Molecular basis of prodrug activation by human valacyclovirase, an alpha-amino acid ester hydrolase.
Lai L, Xu Z, Zhou J, Lee KD, Amidon GL
The Journal of biological chemistry283(14)9318-27(2008 Apr)

Structure and specificity of a human valacyclovir activating enzyme: a homology model of BPHL.
Kim I, Crippen GM, Amidon GL
Molecular pharmaceutics1(6)434-46(2004 Nov-Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The catalytic efficiency (kcat/Km) of rBPHL for homocysteine thiolactone hydrolysis was 7.7 x 10(4) M(-1)s(-1), orders of magnitude higher than that of PON1 or Blmh, indicating a more significant physiological role for BPHL in detoxifying HCTL
Title: Human valacyclovir hydrolase/biphenyl hydrolase-like protein is a highly efficient homocysteine thiolactonase.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

Observational study of gene-disease association. (HuGE Navigator)
Title: New genetic associations detected in a host response study to hepatitis B vaccine.

BPHL catalyzes the hydrolytic activation of the antiviral prodrugs valacyclovir and valganciclovir.
Title: A novel nucleoside prodrug-activating enzyme: substrate specificity of biphenyl hydrolase-like protein.

BPHL is a serine hydrolase that catalyzes the hydrolytic activation of amino acid ester prodrugs of nuceloside analogs such as valacyclovir and valganciclovir.
Title: Identification of a human valacyclovirase: biphenyl hydrolase-like protein as valacyclovir hydrolase.

The following BPHL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BPHL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu14842	NM_004332.4 Latest version!	Homo sapiens biphenyl hydrolase like (BPHL), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu56515	NM_001302777.1 Latest version!	Homo sapiens biphenyl hydrolase like (BPHL), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
View Old Accession Versions >>
OHu14842	NM_004332.3	Homo sapiens biphenyl hydrolase like (BPHL), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BPHL ( NM_004332.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004323.2 Homo sapiens biphenyl hydrolase like (BPHL), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu14842
Clone ID Related Accession (Same CDS sequence)	NM_004332.3 , NM_004332.4
Accession Version	NM_004332.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	valacyclovir hydrolase isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC037778.1, AL031963.40, BC106901.1, AJ617684.1 and BM977067.1. On Nov 14, 2014 this sequence version replaced NM_004332.2. Transcript Variant: This variant (1) represents the shortest transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.197889.1, SRR1803617.152453.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGTGGCTG TGCTGGGCGG CCGGGGCGTG TTGCGCCTGC GGCTGCTTCT CTCAGCGCTG 
AAGCCCGGGA TCCACGTCCC ACGGGCCGGA CCCGCGGCCG CGTTCGGCAC CTCGGTAACC 
TCTGCCAAAG TGGCTGTGAA TGGCGTTCAG CTGCATTACC AGCAGACTGG AGAGGGAGAT 
CACGCAGTCC TGCTACTTCC TGGGATGTTA GGAAGTGGAG AGACTGATTT TGGACCTCAG 
CTCAAGAACC TCAATAAGAA GCTCTTCACG GTGGTCGCCT GGGATCCTCG AGGCTATGGA 
CATTCCAGGC CCCCAGATCG CGATTTCCCA GCAGACTTTT TTGAAAGGGA TGCAAAAGAT 
GCTGTTGATT TGATGAAGGC GCTGAAGTTT AAGAAGGTTT CTCTGCTGGG GTGGAGTGAT 
GGGGGCATAA CCGCACTCAT TGCTGCTGCA AAATATCCAT CTTACATCCA CAAGATGGTG 
ATCTGGGGCG CCAACGCCTA CGTCACTGAC GAAGACAGCA TGATATATGA GGGCATCCGA 
GATGTTTCCA AATGGAGTGA GAGAACAAGA AAGCCTCTAG AAGCCCTCTA TGGGTATGAC 
TACTTTGCCA GAACCTGTGA AAAGTGGGTG GATGGCATAA GACAGTTTAA ACATCTCCCA 
GATGGTAACA TCTGCCGGCA CCTGCTGCCC CGGGTCCAGT GCCCCGCCTT GATTGTGCAC 
GGTGAGAAGG ATCCTCTGGT CCCACGGTTT CATGCCGACT TCATTCATAA GCACGTGAAA 
GGCTCACGGC TGCATTTGAT GCCAGAAGGC AAACACAACC TGCATTTGCG TTTTGCAGAT 
GAATTCAACA AGTTAGCAGA AGACTTCCTA CAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004323.2
CDS	366..1241
Translation	MVAVLGGRGVLRLRLLLSALKPGIHVPRAGPAAAFGTSVTSAKVAVNGVQLHYQQTGEGDHAVLLLPGMLGSGETDFGPQLKNLNKKLFTVVAWDPRGYGHSRPPDRDFPADFFERDAKDAVDLMKALKFKKVSLLGWSDGGITALIAAAKYPSYIHKMVIWGANAYVTDEDSMIYEGIRDVSKWSERTRKPLEALYGYDYFARTCEKWVDGIRQFKHLPDGNICRHLLPRVQCPALIVHGEKDPLVPRFHADFIHKHVKGSRLHLMPEGKHNLHLRFADEFNKLAEDFLQ

Target ORF information:

RefSeq Version	NM_004332.3
Organism	Homo sapiens(human)
Definition	Homo sapiens biphenyl hydrolase like (BPHL), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004332.3

ORF Insert Sequence:

ATGGTGGCTG TGCTGGGCGG CCGGGGCGTG TTGCGCCTGC GGCTGCTTCT CTCAGCGCTG 
AAGCCCGGGA TCCACGTCCC ACGGGCCGGA CCCGCGGCCG CGTTCGGCAC CTCGGTAACC 
TCTGCCAAAG TGGCTGTGAA TGGCGTTCAG CTGCATTACC AGCAGACTGG AGAGGGAGAT 
CACGCAGTCC TGCTACTTCC TGGGATGTTA GGAAGTGGAG AGACTGATTT TGGACCTCAG 
CTCAAGAACC TCAATAAGAA GCTCTTCACG GTGGTCGCCT GGGATCCTCG AGGCTATGGA 
CATTCCAGGC CCCCAGATCG CGATTTCCCA GCAGACTTTT TTGAAAGGGA TGCAAAAGAT 
GCTGTTGATT TGATGAAGGC GCTGAAGTTT AAGAAGGTTT CTCTGCTGGG GTGGAGTGAT 
GGGGGCATAA CCGCACTCAT TGCTGCTGCA AAATATCCAT CTTACATCCA CAAGATGGTG 
ATCTGGGGCG CCAACGCCTA CGTCACTGAC GAAGACAGCA TGATATATGA GGGCATCCGA 
GATGTTTCCA AATGGAGTGA GAGAACAAGA AAGCCTCTAG AAGCCCTCTA TGGGTATGAC 
TACTTTGCCA GAACCTGTGA AAAGTGGGTG GATGGCATAA GACAGTTTAA ACATCTCCCA 
GATGGTAACA TCTGCCGGCA CCTGCTGCCC CGGGTCCAGT GCCCCGCCTT GATTGTGCAC 
GGTGAGAAGG ATCCTCTGGT CCCACGGTTT CATGCCGACT TCATTCATAA GCACGTGAAA 
GGCTCACGGC TGCATTTGAT GCCAGAAGGC AAACACAACC TGCATTTGCG TTTTGCAGAT 
GAATTCAACA AGTTAGCAGA AGACTTCCTA CAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BPHL ( NM_004332.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004323.2 Homo sapiens biphenyl hydrolase like (BPHL), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu14842
Clone ID Related Accession (Same CDS sequence)	NM_004332.3 , NM_004332.4
Accession Version	NM_004332.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	valacyclovir hydrolase isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC037778.1, AL031963.40, BC106901.1, AJ617684.1 and BM977067.1. On Nov 25, 2018 this sequence version replaced NM_004332.3. Transcript Variant: This variant (1) represents the shortest transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.238312.1, SRR1660807.222525.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000380379.10/ ENSP00000369739.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTGGCTG TGCTGGGCGG CCGGGGCGTG TTGCGCCTGC GGCTGCTTCT CTCAGCGCTG 
AAGCCCGGGA TCCACGTCCC ACGGGCCGGA CCCGCGGCCG CGTTCGGCAC CTCGGTAACC 
TCTGCCAAAG TGGCTGTGAA TGGCGTTCAG CTGCATTACC AGCAGACTGG AGAGGGAGAT 
CACGCAGTCC TGCTACTTCC TGGGATGTTA GGAAGTGGAG AGACTGATTT TGGACCTCAG 
CTCAAGAACC TCAATAAGAA GCTCTTCACG GTGGTCGCCT GGGATCCTCG AGGCTATGGA 
CATTCCAGGC CCCCAGATCG CGATTTCCCA GCAGACTTTT TTGAAAGGGA TGCAAAAGAT 
GCTGTTGATT TGATGAAGGC GCTGAAGTTT AAGAAGGTTT CTCTGCTGGG GTGGAGTGAT 
GGGGGCATAA CCGCACTCAT TGCTGCTGCA AAATATCCAT CTTACATCCA CAAGATGGTG 
ATCTGGGGCG CCAACGCCTA CGTCACTGAC GAAGACAGCA TGATATATGA GGGCATCCGA 
GATGTTTCCA AATGGAGTGA GAGAACAAGA AAGCCTCTAG AAGCCCTCTA TGGGTATGAC 
TACTTTGCCA GAACCTGTGA AAAGTGGGTG GATGGCATAA GACAGTTTAA ACATCTCCCA 
GATGGTAACA TCTGCCGGCA CCTGCTGCCC CGGGTCCAGT GCCCCGCCTT GATTGTGCAC 
GGTGAGAAGG ATCCTCTGGT CCCACGGTTT CATGCCGACT TCATTCATAA GCACGTGAAA 
GGCTCACGGC TGCATTTGAT GCCAGAAGGC AAACACAACC TGCATTTGCG TTTTGCAGAT 
GAATTCAACA AGTTAGCAGA AGACTTCCTA CAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004323.2
CDS	31..906
Translation	MVAVLGGRGVLRLRLLLSALKPGIHVPRAGPAAAFGTSVTSAKVAVNGVQLHYQQTGEGDHAVLLLPGMLGSGETDFGPQLKNLNKKLFTVVAWDPRGYGHSRPPDRDFPADFFERDAKDAVDLMKALKFKKVSLLGWSDGGITALIAAAKYPSYIHKMVIWGANAYVTDEDSMIYEGIRDVSKWSERTRKPLEALYGYDYFARTCEKWVDGIRQFKHLPDGNICRHLLPRVQCPALIVHGEKDPLVPRFHADFIHKHVKGSRLHLMPEGKHNLHLRFADEFNKLAEDFLQ

Target ORF information:

RefSeq Version	NM_004332.4
Organism	Homo sapiens(human)
Definition	Homo sapiens biphenyl hydrolase like (BPHL), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004332.4

ORF Insert Sequence:

ATGGTGGCTG TGCTGGGCGG CCGGGGCGTG TTGCGCCTGC GGCTGCTTCT CTCAGCGCTG 
AAGCCCGGGA TCCACGTCCC ACGGGCCGGA CCCGCGGCCG CGTTCGGCAC CTCGGTAACC 
TCTGCCAAAG TGGCTGTGAA TGGCGTTCAG CTGCATTACC AGCAGACTGG AGAGGGAGAT 
CACGCAGTCC TGCTACTTCC TGGGATGTTA GGAAGTGGAG AGACTGATTT TGGACCTCAG 
CTCAAGAACC TCAATAAGAA GCTCTTCACG GTGGTCGCCT GGGATCCTCG AGGCTATGGA 
CATTCCAGGC CCCCAGATCG CGATTTCCCA GCAGACTTTT TTGAAAGGGA TGCAAAAGAT 
GCTGTTGATT TGATGAAGGC GCTGAAGTTT AAGAAGGTTT CTCTGCTGGG GTGGAGTGAT 
GGGGGCATAA CCGCACTCAT TGCTGCTGCA AAATATCCAT CTTACATCCA CAAGATGGTG 
ATCTGGGGCG CCAACGCCTA CGTCACTGAC GAAGACAGCA TGATATATGA GGGCATCCGA 
GATGTTTCCA AATGGAGTGA GAGAACAAGA AAGCCTCTAG AAGCCCTCTA TGGGTATGAC 
TACTTTGCCA GAACCTGTGA AAAGTGGGTG GATGGCATAA GACAGTTTAA ACATCTCCCA 
GATGGTAACA TCTGCCGGCA CCTGCTGCCC CGGGTCCAGT GCCCCGCCTT GATTGTGCAC 
GGTGAGAAGG ATCCTCTGGT CCCACGGTTT CATGCCGACT TCATTCATAA GCACGTGAAA 
GGCTCACGGC TGCATTTGAT GCCAGAAGGC AAACACAACC TGCATTTGCG TTTTGCAGAT 
GAATTCAACA AGTTAGCAGA AGACTTCCTA CAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BPHL ( NM_001302777.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001289706.1 Homo sapiens biphenyl hydrolase like (BPHL), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu56515
Clone ID Related Accession (Same CDS sequence)	NM_001302777.1
Accession Version	NM_001302777.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	valacyclovir hydrolase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC037778.1, AL031963.40, BC106901.1, AJ617684.1 and BM977067.1. On Nov 14, 2014 this sequence version replaced NR_026650.1. Transcript Variant: This variant (3) includes an alternate exon in its 5' coding region and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.3462.1, SRR1660809.219989.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCAGGA ATCTGCTTTA TTCTCTTTTG TCCTCCCACC TGTCCCCCCA TTTCAGCACC 
TCGGTAACCT CTGCCAAAGT GGCTGTGAAT GGCGTTCAGC TGCATTACCA GCAGACTGGA 
GAGGGAGATC ACGCAGTCCT GCTACTTCCT GGGATGTTAG GAAGTGGAGA GACTGATTTT 
GGACCTCAGC TCAAGAACCT CAATAAGAAG CTCTTCACGG TGGTCGCCTG GGATCCTCGA 
GGCTATGGAC ATTCCAGGCC CCCAGATCGC GATTTCCCAG CAGACTTTTT TGAAAGGGAT 
GCAAAAGATG CTGTTGATTT GATGAAGGCG CTGAAGTTTA AGAAGGTTTC TCTGCTGGGG 
TGGAGTGATG GGGGCATAAC CGCACTCATT GCTGCTGCAA AATATCCATC TTACATCCAC 
AAGATGGTGA TCTGGGGCGC CAACGCCTAC GTCACTGACG AAGACAGCAT GATATATGAG 
GGCATCCGAG ATGTTTCCAA ATGGAGTGAG AGAACAAGAA AGCCTCTAGA AGCCCTCTAT 
GGGTATGACT ACTTTGCCAG AACCTGTGAA AAGTGGGTGG ATGGCATAAG ACAGTTTAAA 
CATCTCCCAG ATGGTAACAT CTGCCGGCAC CTGCTGCCCC GGGTCCAGTG CCCCGCCTTG 
ATTGTGCACG GTGAGAAGGA TCCTCTGGTC CCACGGTTTC ATGCCGACTT CATTCATAAG 
CACGTGAAAG GCTCACGGCT GCATTTGATG CCAGAAGGCA AACACAACCT GCATTTGCGT 
TTTGCAGATG AATTCAACAA GTTAGCAGAA GACTTCCTAC AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289706.1
CDS	645..1469
Translation	MPRNLLYSLLSSHLSPHFSTSVTSAKVAVNGVQLHYQQTGEGDHAVLLLPGMLGSGETDFGPQLKNLNKKLFTVVAWDPRGYGHSRPPDRDFPADFFERDAKDAVDLMKALKFKKVSLLGWSDGGITALIAAAKYPSYIHKMVIWGANAYVTDEDSMIYEGIRDVSKWSERTRKPLEALYGYDYFARTCEKWVDGIRQFKHLPDGNICRHLLPRVQCPALIVHGEKDPLVPRFHADFIHKHVKGSRLHLMPEGKHNLHLRFADEFNKLAEDFLQ

Target ORF information:

RefSeq Version	NM_001302777.1
Organism	Homo sapiens(human)
Definition	Homo sapiens biphenyl hydrolase like (BPHL), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001302777.1

ORF Insert Sequence:

ATGCCCAGGA ATCTGCTTTA TTCTCTTTTG TCCTCCCACC TGTCCCCCCA TTTCAGCACC 
TCGGTAACCT CTGCCAAAGT GGCTGTGAAT GGCGTTCAGC TGCATTACCA GCAGACTGGA 
GAGGGAGATC ACGCAGTCCT GCTACTTCCT GGGATGTTAG GAAGTGGAGA GACTGATTTT 
GGACCTCAGC TCAAGAACCT CAATAAGAAG CTCTTCACGG TGGTCGCCTG GGATCCTCGA 
GGCTATGGAC ATTCCAGGCC CCCAGATCGC GATTTCCCAG CAGACTTTTT TGAAAGGGAT 
GCAAAAGATG CTGTTGATTT GATGAAGGCG CTGAAGTTTA AGAAGGTTTC TCTGCTGGGG 
TGGAGTGATG GGGGCATAAC CGCACTCATT GCTGCTGCAA AATATCCATC TTACATCCAC 
AAGATGGTGA TCTGGGGCGC CAACGCCTAC GTCACTGACG AAGACAGCAT GATATATGAG 
GGCATCCGAG ATGTTTCCAA ATGGAGTGAG AGAACAAGAA AGCCTCTAGA AGCCCTCTAT 
GGGTATGACT ACTTTGCCAG AACCTGTGAA AAGTGGGTGG ATGGCATAAG ACAGTTTAAA 
CATCTCCCAG ATGGTAACAT CTGCCGGCAC CTGCTGCCCC GGGTCCAGTG CCCCGCCTTG 
ATTGTGCACG GTGAGAAGGA TCCTCTGGTC CCACGGTTTC ATGCCGACTT CATTCATAAG 
CACGTGAAAG GCTCACGGCT GCATTTGATG CCAGAAGGCA AACACAACCT GCATTTGCGT 
TTTGCAGATG AATTCAACAA GTTAGCAGAA GACTTCCTAC AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Human valacyclovir hydrolase/biphenyl hydrolase-like protein is a highly efficient homocysteine thiolactonase.
PloS one9(10)e110054(2014)
Marsillach J,Suzuki SM,Richter RJ,McDonald MG,Rademacher PM,MacCoss MJ,Hsieh EJ,Rettie AE,Furlong CE

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
PloS one5(9)e12862(2010 Sep)
Hendrickson SL,Lautenberger JA,Chinn LW,Malasky M,Sezgin E,Kingsley LA,Goedert JJ,Kirk GD,Gomperts ED,Buchbinder SP,Troyer JL,O'Brien SJ

New genetic associations detected in a host response study to hepatitis B vaccine.
Genes and immunity11(3)232-8(2010 Apr)
Davila S,Froeling FE,Tan A,Bonnard C,Boland GJ,Snippe H,Hibberd ML,Seielstad M

Molecular basis of prodrug activation by human valacyclovirase, an alpha-amino acid ester hydrolase.
The Journal of biological chemistry283(14)9318-27(2008 Apr)
Lai L,Xu Z,Zhou J,Lee KD,Amidon GL

Structure and specificity of a human valacyclovir activating enzyme: a homology model of BPHL.
Molecular pharmaceutics1(6)434-46(2004 Nov-Dec)
Kim I,Crippen GM,Amidon GL