SPINK1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPINK1
Entrez Gene ID	6690
Full Name	serine peptidase inhibitor, Kazal type 1
Synonyms	PCTT,PSTI,Spink3,TATI,TCP
General protein information	Preferred Names serine peptidase inhibitor, Kazal type 1 Names serine protease inhibitor Kazal-type 1 pancreatic secretory trypsin inhibitor serine protease inhibitor, Kazal type 1 tumor-associated trypsin inhibitor
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q32
Summary	The protein encoded by this gene is a trypsin inhibitor, which is secreted from pancreatic acinar cells into pancreatic juice. It is thought to function in the prevention of trypsin-catalyzed premature activation of zymogens within the pancreas and the pancreatic duct. Mutations in this gene are associated with hereditary pancreatitis and tropical calcific pancreatitis. [provided by RefSeq, Oct 2008].
Disorder MIM:	167790
Disorder Html:	Pancreatitis, hereditary, 167800 (3); {Fibrocalculous pancreatic diabetes, susceptibility to}, 608189 (3); Tropical calcific pancreatitis, 608189 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_003122.4	NP_003113.2	serine protease inhibitor Kazal-type 1 precursor
NM_001354966.1	NP_001341895.1	serine protease inhibitor Kazal-type 1 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SPINK1	XP_001160275.1
	SPINK1	XP_001102888.1
Bos taurus (cattle)	SPINK1	NP_001020519.1
Mus musculus (house mouse)	Spink3	NP_033284.1
Homo sapiens (human)	SPINK1	NP_003113.2
Canis lupus familiaris (dog)	SPINK1	XP_850557.1

Gene Ontology

The following SPINK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPINK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu28321	NM_001354966.1 Latest version!	Homo sapiens serine peptidase inhibitor Kazal type 1 (SPINK1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu28321	NM_003122.4 Latest version!	Homo sapiens serine peptidase inhibitor Kazal type 1 (SPINK1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPINK1 ( NM_003122.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003113.2 Homo sapiens serine peptidase inhibitor Kazal type 1 (SPINK1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu28321
Clone ID Related Accession (Same CDS sequence)	NM_003122.4 , NM_001354966.1
Accession Version	NM_003122.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-01
Organism	Homo sapiens(human)
Product	serine protease inhibitor Kazal-type 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB159576.1, BC025790.1 and BU074107.1. This sequence is a reference standard in the RefSeqGene project. On or before Jun 20, 2014 this sequence version replaced XM_005268501.1, NM_003122.3. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CB159576.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGGTAA CAGGCATCTT TCTTCTCAGT GCCTTGGCCC TGTTGAGTCT ATCTGGTAAC 
ACTGGAGCTG ACTCCCTGGG AAGAGAGGCC AAATGTTACA ATGAACTTAA TGGATGCACC 
AAGATATATG ACCCTGTCTG TGGGACTGAT GGAAATACTT ATCCCAATGA ATGCGTGTTA 
TGTTTTGAAA ATCGGAAACG CCAGACTTCT ATCCTCATTC AAAAATCTGG GCCTTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003113.2
CDS	290..529
Translation	MKVTGIFLLSALALLSLSGNTGADSLGREAKCYNELNGCTKIYDPVCGTDGNTYPNECVLCFENRKRQTSILIQKSGPC

Target ORF information:

RefSeq Version	NM_003122.4
Organism	Homo sapiens(human)
Definition	Homo sapiens serine peptidase inhibitor Kazal type 1 (SPINK1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003122.4

ORF Insert Sequence:

ATGAAGGTAA CAGGCATCTT TCTTCTCAGT GCCTTGGCCC TGTTGAGTCT ATCTGGTAAC 
ACTGGAGCTG ACTCCCTGGG AAGAGAGGCC AAATGTTACA ATGAACTTAA TGGATGCACC 
AAGATATATG ACCCTGTCTG TGGGACTGAT GGAAATACTT ATCCCAATGA ATGCGTGTTA 
TGTTTTGAAA ATCGGAAACG CCAGACTTCT ATCCTCATTC AAAAATCTGG GCCTTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPINK1 ( NM_001354966.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001341895.1 Homo sapiens serine peptidase inhibitor Kazal type 1 (SPINK1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu28321
Clone ID Related Accession (Same CDS sequence)	NM_003122.4 , NM_001354966.1
Accession Version	NM_001354966.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Homo sapiens(human)
Product	serine protease inhibitor Kazal-type 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC011352.6. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279876.306.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA2144335 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAAGGTAA CAGGCATCTT TCTTCTCAGT GCCTTGGCCC TGTTGAGTCT ATCTGGTAAC 
ACTGGAGCTG ACTCCCTGGG AAGAGAGGCC AAATGTTACA ATGAACTTAA TGGATGCACC 
AAGATATATG ACCCTGTCTG TGGGACTGAT GGAAATACTT ATCCCAATGA ATGCGTGTTA 
TGTTTTGAAA ATCGGAAACG CCAGACTTCT ATCCTCATTC AAAAATCTGG GCCTTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001341895.1
CDS	323..562
Translation	MKVTGIFLLSALALLSLSGNTGADSLGREAKCYNELNGCTKIYDPVCGTDGNTYPNECVLCFENRKRQTSILIQKSGPC

Target ORF information:

RefSeq Version	NM_001354966.1
Organism	Homo sapiens(human)
Definition	Homo sapiens serine peptidase inhibitor Kazal type 1 (SPINK1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001354966.1

ORF Insert Sequence:

ATGAAGGTAA CAGGCATCTT TCTTCTCAGT GCCTTGGCCC TGTTGAGTCT ATCTGGTAAC 
ACTGGAGCTG ACTCCCTGGG AAGAGAGGCC AAATGTTACA ATGAACTTAA TGGATGCACC 
AAGATATATG ACCCTGTCTG TGGGACTGAT GGAAATACTT ATCCCAATGA ATGCGTGTTA 
TGTTTTGAAA ATCGGAAACG CCAGACTTCT ATCCTCATTC AAAAATCTGG GCCTTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.