ST3GAL2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ST3GAL2
Entrez Gene ID	6483
Full Name	ST3 beta-galactoside alpha-2,3-sialyltransferase 2
Synonyms	Gal-NAc6S,SIAT4B,ST3GALII,ST3GalA.2
General protein information	Preferred Names ST3 beta-galactoside alpha-2,3-sialyltransferase 2 Names CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 2 Gal-beta-1,3-GalNAc-alpha-2,3-sialyltransferase SIAT4-B ST3Gal II alpha 2,3-ST 2 beta-galactoside alpha-2,3-sialyltransferase 2 sialyltransferase 4B (beta-galactosidase alpha-2,3-sialytransferase)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16q22.1
Summary	The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein is normally found in the Golgi but can be proteolytically processed to a soluble form. This protein, which is a member of glycosyltransferase family 29, can use the same acceptor substrates as does sialyltransferase 4A. [provided by RefSeq, Jul 2008].
Disorder MIM:	607188

mRNA and Protein(s)

mRNA	Protein	Name
NM_006927.3	NP_008858.1	CMP-N-acetylneuraminate-beta-galactosamide- alpha-2,3-sialyltransferase 2
NM_006927.4	NP_008858.1	CMP-N-acetylneuraminate-beta-galactosamide- alpha-2,3-sialyltransferase 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	ST3GAL2	NP_008858.1
Danio rerio (zebrafish)	LOC101882979	XP_005159216.1
Pan troglodytes (chimpanzee)	ST3GAL2	NP_001032384.1
Canis lupus familiaris (dog)	ST3GAL2	XP_546834.2
Xenopus tropicalis (tropical clawed frog)	st3gal2	XP_002931706.1
	ST3GAL2	XP_001107274.1
Gallus gallus (chicken)	ST3GAL2	NP_989811.2
Mus musculus (house mouse)	St3gal2	NP_033205.2
Danio rerio (zebrafish)	st3gal2	NP_001004012.1
Bos taurus (cattle)	ST3GAL2	NP_001002892.1
Rattus norvegicus (Norway rat)	St3gal2	NP_113883.2

Gene Ontology
Bibliography

Related articles in PubMed

Ganglioside glycosyltransferases are S-acylated at conserved cysteine residues involved in homodimerisation.
Chumpen Ramirez S, Ruggiero FM, Daniotti JL, Valdez Taubas J
The Biochemical journal474(16)2803-2816(2017 08)

Critical role of evolutionarily conserved glycosylation at Asn211 in the intracellular trafficking and activity of sialyltransferase ST3Gal-II.
Ruggiero FM, Vilcaes AA, Iglesias-Bartolom? R, Daniotti JL
The Biochemical journal469(1)83-95(2015 Jul)

Human alpha2,3-sialyltransferase (ST3Gal II) is a stage-specific embryonic antigen-4 synthase.
Saito S, Aoki H, Ito A, Ueno S, Wada T, Mitsuzuka K, Satoh M, Arai Y, Miyagi T
The Journal of biological chemistry278(29)26474-9(2003 Jul)

Genomic structure, expression, and transcriptional regulation of human Gal beta 1,3 GalNAc alpha 2,3-sialyltransferase gene.
Taniguchi A, Morishima T, Tsujita Y, Matsumoto Y, Matsumoto K
Biochemical and biophysical research communications300(2)570-6(2003 Jan)

Cloning and expression of cDNA for a human Gal(beta1-3)GalNAc alpha2,3-sialyltransferase from the CEM T-cell line.
Giordanengo V, Bannwarth S, Laffont C, Van Miegem V, Harduin-Lepers A, Delannoy P, Lefebvre JC
European journal of biochemistry247(2)558-66(1997 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that ganglioside glycosyltransferases ST3GAL5, ST8SIA1, and B4GALNT1 are S-acylated at conserved cysteine residues located close to cytoplasmic border of their transmembrane domains; ST3Gal-II is acylated at conserved cysteine residue in N-terminal cytoplasmic tail; for B4GALNT1 and ST3Gal-II, dimer formation controls their S-acylation status.
Title: Ganglioside glycosyltransferases are S-acylated at conserved cysteine residues involved in homodimerisation.

This suggests that the C-terminal domain of ST3Gal-II depends on N-glycosylation to attain an optimum conformation for proper exit from the endoplasmic reticulum
Title: Critical role of evolutionarily conserved glycosylation at Asn211 in the intracellular trafficking and activity of sialyltransferase ST3Gal-II.

ST3Gal II is a MSGb5 (stage-specific embryonic antigen-4) synthase and its increased expression level is closely related to renal carcinogenesis
Title: Human alpha2,3-sialyltransferase (ST3Gal II) is a stage-specific embryonic antigen-4 synthase.

Genomic structure, expression, and transcriptional regulation of ST3GALII.
Title: Genomic structure, expression, and transcriptional regulation of human Gal beta 1,3 GalNAc alpha 2,3-sialyltransferase gene.

The following ST3GAL2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ST3GAL2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu05864	NM_006927.4 Latest version!	Homo sapiens ST3 beta-galactoside alpha-2,3-sialyltransferase 2 (ST3GAL2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu05864	NM_006927.3	Homo sapiens ST3 beta-galactoside alpha-2,3-sialyltransferase 2 (ST3GAL2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ST3GAL2 ( NM_006927.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_008858.1 Homo sapiens ST3 beta-galactoside alpha-2,3-sialyltransferase 2 (ST3GAL2), mRNA.

Price & Availability↑

CloneID	OHu05864
Clone ID Related Accession (Same CDS sequence)	NM_006927.3 , NM_006927.4
Accession Version	NM_006927.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	CMP-N-acetylneuraminate-beta-galactosamide- alpha-2,3-sialyltransferase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC012184.7, AK127322.1 and BC036777.1. This sequence is a reference standard in the RefSeqGene project. On or before Aug 31, 2013 this sequence version replaced XM_005256114.1, XM_001130512.1, NM_006927.2. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.40910.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGAAGTGCT CCCTGCGGGT GTGGTTCCTC TCCGTGGCCT TCCTGCTGGT GTTCATCATG 
TCCCTGCTCT TCACCTACTC GCACCACAGC ATGGCCACGC TCCCCTACCT GGACTCAGGG 
GCCCTGGATG GGACGCACCG GGTGAAGCTG GTGCCCGGCT ATGCCGGCCT GCAGCGCCTC 
AGCAAGGAGA GGCTCTCGGG CAAGAGCTGT GCCTGTCGCC GCTGCATGGG CGATGCCGGT 
GCCTCCGACT GGTTTGACAG CCACTTTGAC GGTAACATTT CCCCCGTCTG GACCCGAGAG 
AACATGGATC TTCCACCGGA CGTCCAGAGG TGGTGGATGA TGCTGCAGCC CCAGTTCAAG 
TCACACAACA CCAATGAGGT GCTGGAGAAG CTGTTCCAGA TAGTGCCTGG CGAGAACCCC 
TACCGCTTCC GGGACCCCCA CCAGTGCCGG CGCTGTGCCG TGGTGGGGAA CTCGGGCAAC 
CTGCGGGGCT CTGGCTATGG GCAGGACGTG GACGGGCACA ACTTCATCAT GAGGATGAAT 
CAGGCGCCAA CCGTGGGCTT TGAGCAGGAT GTTGGCAGCC GAACCACCCA CCATTTCATG 
TACCCTGAGA GTGCCAAGAA CCTGCCCGCC AACGTCAGCT TCGTGCTGGT GCCCTTCAAG 
GTCCTGGACC TTCTGTGGAT CGCCAGCGCC TTGTCCACGG GGCAGATCCG ATTCACCTAC 
GCCCCAGTGA AGTCCTTCCT TCGAGTGGAT AAAGAAAAGG TCCAGATCTA CAACCCAGCC 
TTCTTCAAGT ATATCCACGA CAGGTGGACA GAGCATCACG GGCGGTACCC TTCCACGGGG 
ATGCTGGTGC TTTTCTTTGC CCTGCATGTG TGTGATGAGG TGAACGTGTA CGGGTTCGGG 
GCCGACAGCC GGGGCAACTG GCACCACTAC TGGGAGAACA ACCGGTACGC GGGCGAGTTC 
CGGAAGACTG GCGTGCACGA CGCGGACTTC GAGGCCCACA TCATCGACAT GCTGGCCAAG 
GCCAGCAAGA TCGAAGTCTA CCGGGGCAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_008858.1
CDS	1144..2196
Translation	MKCSLRVWFLSVAFLLVFIMSLLFTYSHHSMATLPYLDSGALDGTHRVKLVPGYAGLQRLSKERLSGKSCACRRCMGDAGASDWFDSHFDGNISPVWTRENMDLPPDVQRWWMMLQPQFKSHNTNEVLEKLFQIVPGENPYRFRDPHQCRRCAVVGNSGNLRGSGYGQDVDGHNFIMRMNQAPTVGFEQDVGSRTTHHFMYPESAKNLPANVSFVLVPFKVLDLLWIASALSTGQIRFTYAPVKSFLRVDKEKVQIYNPAFFKYIHDRWTEHHGRYPSTGMLVLFFALHVCDEVNVYGFGADSRGNWHHYWENNRYAGEFRKTGVHDADFEAHIIDMLAKASKIEVYRGN

Target ORF information:

RefSeq Version	NM_006927.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ST3 beta-galactoside alpha-2,3-sialyltransferase 2 (ST3GAL2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006927.3

ORF Insert Sequence:

ATGAAGTGCT CCCTGCGGGT GTGGTTCCTC TCCGTGGCCT TCCTGCTGGT GTTCATCATG 
TCCCTGCTCT TCACCTACTC GCACCACAGC ATGGCCACGC TCCCCTACCT GGACTCAGGG 
GCCCTGGATG GGACGCACCG GGTGAAGCTG GTGCCCGGCT ATGCCGGCCT GCAGCGCCTC 
AGCAAGGAGA GGCTCTCGGG CAAGAGCTGT GCCTGTCGCC GCTGCATGGG CGATGCCGGT 
GCCTCCGACT GGTTTGACAG CCACTTTGAC GGTAACATTT CCCCCGTCTG GACCCGAGAG 
AACATGGATC TTCCACCGGA CGTCCAGAGG TGGTGGATGA TGCTGCAGCC CCAGTTCAAG 
TCACACAACA CCAATGAGGT GCTGGAGAAG CTGTTCCAGA TAGTGCCTGG CGAGAACCCC 
TACCGCTTCC GGGACCCCCA CCAGTGCCGG CGCTGTGCCG TGGTGGGGAA CTCGGGCAAC 
CTGCGGGGCT CTGGCTATGG GCAGGACGTG GACGGGCACA ACTTCATCAT GAGGATGAAT 
CAGGCGCCAA CCGTGGGCTT TGAGCAGGAT GTTGGCAGCC GAACCACCCA CCATTTCATG 
TACCCTGAGA GTGCCAAGAA CCTGCCCGCC AACGTCAGCT TCGTGCTGGT GCCCTTCAAG 
GTCCTGGACC TTCTGTGGAT CGCCAGCGCC TTGTCCACGG GGCAGATCCG ATTCACCTAC 
GCCCCAGTGA AGTCCTTCCT TCGAGTGGAT AAAGAAAAGG TCCAGATCTA CAACCCAGCC 
TTCTTCAAGT ATATCCACGA CAGGTGGACA GAGCATCACG GGCGGTACCC TTCCACGGGG 
ATGCTGGTGC TTTTCTTTGC CCTGCATGTG TGTGATGAGG TGAACGTGTA CGGGTTCGGG 
GCCGACAGCC GGGGCAACTG GCACCACTAC TGGGAGAACA ACCGGTACGC GGGCGAGTTC 
CGGAAGACTG GCGTGCACGA CGCGGACTTC GAGGCCCACA TCATCGACAT GCTGGCCAAG 
GCCAGCAAGA TCGAAGTCTA CCGGGGCAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ST3GAL2 ( NM_006927.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_008858.1 Homo sapiens ST3 beta-galactoside alpha-2,3-sialyltransferase 2 (ST3GAL2), mRNA.

Price & Availability↑

CloneID	OHu05864
Clone ID Related Accession (Same CDS sequence)	NM_006927.3 , NM_006927.4
Accession Version	NM_006927.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	CMP-N-acetylneuraminate-beta-galactosamide- alpha-2,3-sialyltransferase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC012184.7, AK127322.1 and BC036777.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_006927.3. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.40910.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000342907.3/ ENSP00000345477.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAAGTGCT CCCTGCGGGT GTGGTTCCTC TCCGTGGCCT TCCTGCTGGT GTTCATCATG 
TCCCTGCTCT TCACCTACTC GCACCACAGC ATGGCCACGC TCCCCTACCT GGACTCAGGG 
GCCCTGGATG GGACGCACCG GGTGAAGCTG GTGCCCGGCT ATGCCGGCCT GCAGCGCCTC 
AGCAAGGAGA GGCTCTCGGG CAAGAGCTGT GCCTGTCGCC GCTGCATGGG CGATGCCGGT 
GCCTCCGACT GGTTTGACAG CCACTTTGAC GGTAACATTT CCCCCGTCTG GACCCGAGAG 
AACATGGATC TTCCACCGGA CGTCCAGAGG TGGTGGATGA TGCTGCAGCC CCAGTTCAAG 
TCACACAACA CCAATGAGGT GCTGGAGAAG CTGTTCCAGA TAGTGCCTGG CGAGAACCCC 
TACCGCTTCC GGGACCCCCA CCAGTGCCGG CGCTGTGCCG TGGTGGGGAA CTCGGGCAAC 
CTGCGGGGCT CTGGCTATGG GCAGGACGTG GACGGGCACA ACTTCATCAT GAGGATGAAT 
CAGGCGCCAA CCGTGGGCTT TGAGCAGGAT GTTGGCAGCC GAACCACCCA CCATTTCATG 
TACCCTGAGA GTGCCAAGAA CCTGCCCGCC AACGTCAGCT TCGTGCTGGT GCCCTTCAAG 
GTCCTGGACC TTCTGTGGAT CGCCAGCGCC TTGTCCACGG GGCAGATCCG ATTCACCTAC 
GCCCCAGTGA AGTCCTTCCT TCGAGTGGAT AAAGAAAAGG TCCAGATCTA CAACCCAGCC 
TTCTTCAAGT ATATCCACGA CAGGTGGACA GAGCATCACG GGCGGTACCC TTCCACGGGG 
ATGCTGGTGC TTTTCTTTGC CCTGCATGTG TGTGATGAGG TGAACGTGTA CGGGTTCGGG 
GCCGACAGCC GGGGCAACTG GCACCACTAC TGGGAGAACA ACCGGTACGC GGGCGAGTTC 
CGGAAGACTG GCGTGCACGA CGCGGACTTC GAGGCCCACA TCATCGACAT GCTGGCCAAG 
GCCAGCAAGA TCGAAGTCTA CCGGGGCAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_008858.1
CDS	1156..2208
Translation	MKCSLRVWFLSVAFLLVFIMSLLFTYSHHSMATLPYLDSGALDGTHRVKLVPGYAGLQRLSKERLSGKSCACRRCMGDAGASDWFDSHFDGNISPVWTRENMDLPPDVQRWWMMLQPQFKSHNTNEVLEKLFQIVPGENPYRFRDPHQCRRCAVVGNSGNLRGSGYGQDVDGHNFIMRMNQAPTVGFEQDVGSRTTHHFMYPESAKNLPANVSFVLVPFKVLDLLWIASALSTGQIRFTYAPVKSFLRVDKEKVQIYNPAFFKYIHDRWTEHHGRYPSTGMLVLFFALHVCDEVNVYGFGADSRGNWHHYWENNRYAGEFRKTGVHDADFEAHIIDMLAKASKIEVYRGN

Target ORF information:

RefSeq Version	NM_006927.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ST3 beta-galactoside alpha-2,3-sialyltransferase 2 (ST3GAL2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006927.4

ORF Insert Sequence:

ATGAAGTGCT CCCTGCGGGT GTGGTTCCTC TCCGTGGCCT TCCTGCTGGT GTTCATCATG 
TCCCTGCTCT TCACCTACTC GCACCACAGC ATGGCCACGC TCCCCTACCT GGACTCAGGG 
GCCCTGGATG GGACGCACCG GGTGAAGCTG GTGCCCGGCT ATGCCGGCCT GCAGCGCCTC 
AGCAAGGAGA GGCTCTCGGG CAAGAGCTGT GCCTGTCGCC GCTGCATGGG CGATGCCGGT 
GCCTCCGACT GGTTTGACAG CCACTTTGAC GGTAACATTT CCCCCGTCTG GACCCGAGAG 
AACATGGATC TTCCACCGGA CGTCCAGAGG TGGTGGATGA TGCTGCAGCC CCAGTTCAAG 
TCACACAACA CCAATGAGGT GCTGGAGAAG CTGTTCCAGA TAGTGCCTGG CGAGAACCCC 
TACCGCTTCC GGGACCCCCA CCAGTGCCGG CGCTGTGCCG TGGTGGGGAA CTCGGGCAAC 
CTGCGGGGCT CTGGCTATGG GCAGGACGTG GACGGGCACA ACTTCATCAT GAGGATGAAT 
CAGGCGCCAA CCGTGGGCTT TGAGCAGGAT GTTGGCAGCC GAACCACCCA CCATTTCATG 
TACCCTGAGA GTGCCAAGAA CCTGCCCGCC AACGTCAGCT TCGTGCTGGT GCCCTTCAAG 
GTCCTGGACC TTCTGTGGAT CGCCAGCGCC TTGTCCACGG GGCAGATCCG ATTCACCTAC 
GCCCCAGTGA AGTCCTTCCT TCGAGTGGAT AAAGAAAAGG TCCAGATCTA CAACCCAGCC 
TTCTTCAAGT ATATCCACGA CAGGTGGACA GAGCATCACG GGCGGTACCC TTCCACGGGG 
ATGCTGGTGC TTTTCTTTGC CCTGCATGTG TGTGATGAGG TGAACGTGTA CGGGTTCGGG 
GCCGACAGCC GGGGCAACTG GCACCACTAC TGGGAGAACA ACCGGTACGC GGGCGAGTTC 
CGGAAGACTG GCGTGCACGA CGCGGACTTC GAGGCCCACA TCATCGACAT GCTGGCCAAG 
GCCAGCAAGA TCGAAGTCTA CCGGGGCAAC TGA