NSUN3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NSUN3
Entrez Gene ID	63899
Full Name	NOP2/Sun RNA methyltransferase family member 3
Synonyms	MST077,MSTP077
General protein information	Preferred Names NOP2/Sun RNA methyltransferase family member 3 Names tRNA (cytosine(34)-C(5))-methyltransferase, mitochondrial NOL1/NOP2/Sun domain family member 3 putative methyltransferase NSUN3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3q11.2
Disorder MIM:	617491

mRNA and Protein(s)

mRNA	Protein	Name
NM_022072.3	NP_071355.1	putative methyltransferase NSUN3
NM_022072.5	NP_071355.1	tRNA (cytosine(34)-C(5))-methyltransferase, mitochondrial
NM_022072.4	NP_071355.1	tRNA (cytosine(34)-C(5))-methyltransferase, mitochondrial
XM_024453699.1	XP_024309467.1	tRNA (cytosine(34)-C(5))-methyltransferase, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NSUN3	XP_849037.1
Bos taurus (cattle)	NSUN3	NP_001069469.1
Mus musculus (house mouse)	Nsun3	NP_849256.1
Xenopus tropicalis (tropical clawed frog)	nsun3	XP_002931821.1
Pan troglodytes (chimpanzee)	NSUN3	XP_516606.2
Gallus gallus (chicken)	NSUN3	XP_416643.3
Homo sapiens (human)	NSUN3	NP_071355.1
Danio rerio (zebrafish)	nsun3	NP_001020473.1
Rattus norvegicus (Norway rat)	Nsun3	XP_002727930.2

Gene Ontology
Bibliography

Related articles in PubMed

NSUN3 and ABH1 modify the wobble position of mt-tRNAMet to expand codon recognition in mitochondrial translation.
Haag S, Sloan KE, Ranjan N, Warda AS, Kretschmer J, Blessing C, H?bner B, Seikowski J, Dennerlein S, Rehling P, Rodnina MV, H?bartner C, Bohnsack MT
The EMBO journal35(19)2104-2119(2016 10)

NSUN3 methylase initiates 5-formylcytidine biogenesis in human mitochondrial tRNA(Met).
Nakano S, Suzuki T, Kawarada L, Iwata H, Asano K, Suzuki T
Nature chemical biology12(7)546-51(2016 07)

Deficient methylation and formylation of mt-tRNA(Met) wobble cytosine in a patient carrying mutations in NSUN3.
Van Haute L, Dietmann S, Kremer L, Hussain S, Pearce SF, Powell CA, Rorbach J, Lantaff R, Blanco S, Sauer S, Kotzaeridou U, Hoffmann GF, Memari Y, Kolb-Kokocinski A, Durbin R, Mayr JA, Frye M, Prokisch H, Minczuk M
Nature communications712039(2016 Jun)

Genome-wide association study identifies 8 novel loci associated with blood pressure responses to interventions in Han Chinese.
He J, Kelly TN, Zhao Q, Li H, Huang J, Wang L, Jaquish CE, Sung YJ, Shimmin LC, Lu F, Mu J, Hu D, Ji X, Shen C, Guo D, Ma J, Wang R, Shen J, Li S, Chen J, Mei H, Chen CS, Chen S, Chen J, Li J, Cao J, Lu X, Wu X, Rice TK, Gu CC, Schwander K, Hamm LL, Liu D, Rao DC, Hixson JE, Gu D
Circulation. Cardiovascular genetics6(6)598-607(2013 Dec)

Coeliac disease-associated risk variants in TNFAIP3 and REL implicate altered NF-kappaB signalling.
Trynka G, Zhernakova A, Romanos J, Franke L, Hunt KA, Turner G, Bruinenberg M, Heap GA, Platteel M, Ryan AW, de Kovel C, Holmes GK, Howdle PD, Walters JR, Sanders DS, Mulder CJ, Mearin ML, Verbeek WH, Trimble V, Stevens FM, Kelleher D, Barisani D, Bardella MT, McManus R, van Heel DA, Wijmenga C
Gut58(8)1078-83(2009 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The authors show that the RNA methyltransferase NSUN3 localises to mitochondria and interacts with mt-tRNA(M)(et) to methylate cytosine 34 (C34) at the wobble position.
Title: NSUN3 and ABH1 modify the wobble position of mt-tRNAMet to expand codon recognition in mitochondrial translation.

The NSUN3-knockout cells showed strong reduction in mitochondrial protein synthesis and reduced oxygen consumption, leading to deficient mitochondrial activity. It reconstituted formation of 5-methylcytidine (m(5)C) at position 34 (m(5)C34) on mt-tRNA(Met) with recombinant NSUN3 in the presence of AdoMet, demonstrating that NSUN3-mediated m(5)C34 formation initiates f(5)C34 biogenesis.
Title: NSUN3 methylase initiates 5-formylcytidine biogenesis in human mitochondrial tRNA(Met).

Observational study of gene-disease association. (HuGE Navigator)
Title: Coeliac disease-associated risk variants in TNFAIP3 and REL implicate altered NF-kappaB signalling.

The following NSUN3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NSUN3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17445	NM_022072.5 Latest version!	Homo sapiens NOP2/Sun RNA methyltransferase 3 (NSUN3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu71142	XM_024453699.1 Latest version!	Homo sapiens NOP2/Sun RNA methyltransferase 3 (NSUN3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OHu17445	NM_022072.3	Homo sapiens NOP2/Sun domain family, member 3 (NSUN3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu17445	NM_022072.4	Homo sapiens NOP2/Sun RNA methyltransferase family member 3 (NSUN3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NSUN3 ( NM_022072.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_071355.1 Homo sapiens NOP2/Sun domain family, member 3 (NSUN3), mRNA.

Price & Availability↑

CloneID	OHu17445
Clone ID Related Accession (Same CDS sequence)	NM_022072.3 , NM_022072.5 , NM_022072.4
Accession Version	NM_022072.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	putative methyltransferase NSUN3
Comment

ATGCTGACCC AGCTGAAAGC AAAATCAGAG GGGAAGCTTG CAAAACAGAT TTGCAAAGTT 
GTGTTGGATC ATTTTGAAAA ACAGTATTCC AAAGAACTCG GAGATGCCTG GAATACAGTA 
AGGGAGATAC TAACATCTCC ATCATGCTGG CAATATGCTG TCCTGCTTAA CCGATTCAAT 
TATCCTTTTG AACTGGAAAA GGATTTACAT TTGAAGGGCT ATCACACACT CTCTCAGGGA 
TCTTTACCCA ACTATCCTAA ATCAGTGAAG TGTTACCTTA GCAGAACTCC GGGCCGAATC 
CCTTCAGAAA GACACCAAAT TGGAAACCTG AAAAAATATT ATCTCCTAAA TGCTGCTTCT 
CTTCTCCCAG TGTTGGCTCT GGAATTAAGG GATGGGGAGA AGGTTCTGGA TCTCTGTGCT 
GCTCCTGGAG GGAAATCAAT AGCTCTGCTG CAGTGTGCTT GTCCAGGTTA TCTTCATTGT 
AATGAATATG ATAGTCTGAG ATTGAGGTGG CTAAGGCAGA CGTTGGAATC TTTCATCCCA 
CAGCCTTTGA TAAATGTAAT TAAAGTGTCT GAATTGGATG GCAGAAAAAT GGGAGATGCC 
CAGCCTGAAA TGTTTGACAA GGTGTTAGTG GATGCTCCGT GTTCAAATGA TCGAAGCTGG 
TTGTTTTCTT CTGACTCTCA GAAGGCATCC TGTAGGATAA GTCAAAGGAG GAATTTGCCT 
CTTCTACAGA TAGAGCTGTT AAGGTCTGCA ATTAAGGCCT TACGTCCTGG AGGGATACTT 
GTATACTCTA CATGCACGCT TTCCAAGGCA GAAAATCAAG ATGTGATCAG TGAAATTTTA 
AACTCCCACG GTAACATCAT GCCTATGGAC ATTAAAGGAA TAGCAAGGAC TTGCTCCCAC 
GACTTCACAT TTGCTCCCAC TGGCCAGGAA TGTGGGCTCT TAGTGATTCC AGATAAGGGC 
AAAGCCTGGG GCCCAATGTA TGTAGCCAAA TTGAAGAAAT CATGGAGCAC AGGAAAATGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071355.1
Translation	MLTQLKAKSEGKLAKQICKVVLDHFEKQYSKELGDAWNTVREILTSPSCWQYAVLLNRFNYPFELEKDLHLKGYHTLSQGSLPNYPKSVKCYLSRTPGRIPSERHQIGNLKKYYLLNAASLLPVLALELRDGEKVLDLCAAPGGKSIALLQCACPGYLHCNEYDSLRLRWLRQTLESFIPQPLINVIKVSELDGRKMGDAQPEMFDKVLVDAPCSNDRSWLFSSDSQKASCRISQRRNLPLLQIELLRSAIKALRPGGILVYSTCTLSKAENQDVISEILNSHGNIMPMDIKGIARTCSHDFTFAPTGQECGLLVIPDKGKAWGPMYVAKLKKSWSTGKW

Target ORF information:

RefSeq Version	NM_022072.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens NOP2/Sun domain family, member 3 (NSUN3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022072.3

ORF Insert Sequence:

ATGCTGACCC AGCTGAAAGC AAAATCAGAG GGGAAGCTTG CAAAACAGAT TTGCAAAGTT 
GTGTTGGATC ATTTTGAAAA ACAGTATTCC AAAGAACTCG GAGATGCCTG GAATACAGTA 
AGGGAGATAC TAACATCTCC ATCATGCTGG CAATATGCTG TCCTGCTTAA CCGATTCAAT 
TATCCTTTTG AACTGGAAAA GGATTTACAT TTGAAGGGCT ATCACACACT CTCTCAGGGA 
TCTTTACCCA ACTATCCTAA ATCAGTGAAG TGTTACCTTA GCAGAACTCC GGGCCGAATC 
CCTTCAGAAA GACACCAAAT TGGAAACCTG AAAAAATATT ATCTCCTAAA TGCTGCTTCT 
CTTCTCCCAG TGTTGGCTCT GGAATTAAGG GATGGGGAGA AGGTTCTGGA TCTCTGTGCT 
GCTCCTGGAG GGAAATCAAT AGCTCTGCTG CAGTGTGCTT GTCCAGGTTA TCTTCATTGT 
AATGAATATG ATAGTCTGAG ATTGAGGTGG CTAAGGCAGA CGTTGGAATC TTTCATCCCA 
CAGCCTTTGA TAAATGTAAT TAAAGTGTCT GAATTGGATG GCAGAAAAAT GGGAGATGCC 
CAGCCTGAAA TGTTTGACAA GGTGTTAGTG GATGCTCCGT GTTCAAATGA TCGAAGCTGG 
TTGTTTTCTT CTGACTCTCA GAAGGCATCC TGTAGGATAA GTCAAAGGAG GAATTTGCCT 
CTTCTACAGA TAGAGCTGTT AAGGTCTGCA ATTAAGGCCT TACGTCCTGG AGGGATACTT 
GTATACTCTA CATGCACGCT TTCCAAGGCA GAAAATCAAG ATGTGATCAG TGAAATTTTA 
AACTCCCACG GTAACATCAT GCCTATGGAC ATTAAAGGAA TAGCAAGGAC TTGCTCCCAC 
GACTTCACAT TTGCTCCCAC TGGCCAGGAA TGTGGGCTCT TAGTGATTCC AGATAAGGGC 
AAAGCCTGGG GCCCAATGTA TGTAGCCAAA TTGAAGAAAT CATGGAGCAC AGGAAAATGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NSUN3 ( NM_022072.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_071355.1 Homo sapiens NOP2/Sun RNA methyltransferase 3 (NSUN3), mRNA.

Price & Availability↑

CloneID	OHu17445
Clone ID Related Accession (Same CDS sequence)	NM_022072.3 , NM_022072.5 , NM_022072.4
Accession Version	NM_022072.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	tRNA (cytosine(34)-C(5))-methyltransferase, mitochondrial
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC020602.1, AC019233.7 and BQ030336.1. On Nov 22, 2018 this sequence version replaced NM_022072.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.205930.1, SRR1803615.125322.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000314622.9/ ENSP00000318986.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTGACCC AGCTGAAAGC AAAATCAGAG GGGAAGCTTG CAAAACAGAT TTGCAAAGTT 
GTGTTGGATC ATTTTGAAAA ACAGTATTCC AAAGAACTCG GAGATGCCTG GAATACAGTA 
AGGGAGATAC TAACATCTCC ATCATGCTGG CAATATGCTG TCCTGCTTAA CCGATTCAAT 
TATCCTTTTG AACTGGAAAA GGATTTACAT TTGAAGGGCT ATCACACACT CTCTCAGGGA 
TCTTTACCCA ACTATCCTAA ATCAGTGAAG TGTTACCTTA GCAGAACTCC GGGCCGAATC 
CCTTCAGAAA GACACCAAAT TGGAAACCTG AAAAAATATT ATCTCCTAAA TGCTGCTTCT 
CTTCTCCCAG TGTTGGCTCT GGAATTAAGG GATGGGGAGA AGGTTCTGGA TCTCTGTGCT 
GCTCCTGGAG GGAAATCAAT AGCTCTGCTG CAGTGTGCTT GTCCAGGTTA TCTTCATTGT 
AATGAATATG ATAGTCTGAG ATTGAGGTGG CTAAGGCAGA CGTTGGAATC TTTCATCCCA 
CAGCCTTTGA TAAATGTAAT TAAAGTGTCT GAATTGGATG GCAGAAAAAT GGGAGATGCC 
CAGCCTGAAA TGTTTGACAA GGTGTTAGTG GATGCTCCGT GTTCAAATGA TCGAAGCTGG 
TTGTTTTCTT CTGACTCTCA GAAGGCATCC TGTAGGATAA GTCAAAGGAG GAATTTGCCT 
CTTCTACAGA TAGAGCTGTT AAGGTCTGCA ATTAAGGCCT TACGTCCTGG AGGGATACTT 
GTATACTCTA CATGCACGCT TTCCAAGGCA GAAAATCAAG ATGTGATCAG TGAAATTTTA 
AACTCCCACG GTAACATCAT GCCTATGGAC ATTAAAGGAA TAGCAAGGAC TTGCTCCCAC 
GACTTCACAT TTGCTCCCAC TGGCCAGGAA TGTGGGCTCT TAGTGATTCC AGATAAGGGC 
AAAGCCTGGG GCCCAATGTA TGTAGCCAAA TTGAAGAAAT CATGGAGCAC AGGAAAATGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071355.1
CDS	67..1089
Translation	MLTQLKAKSEGKLAKQICKVVLDHFEKQYSKELGDAWNTVREILTSPSCWQYAVLLNRFNYPFELEKDLHLKGYHTLSQGSLPNYPKSVKCYLSRTPGRIPSERHQIGNLKKYYLLNAASLLPVLALELRDGEKVLDLCAAPGGKSIALLQCACPGYLHCNEYDSLRLRWLRQTLESFIPQPLINVIKVSELDGRKMGDAQPEMFDKVLVDAPCSNDRSWLFSSDSQKASCRISQRRNLPLLQIELLRSAIKALRPGGILVYSTCTLSKAENQDVISEILNSHGNIMPMDIKGIARTCSHDFTFAPTGQECGLLVIPDKGKAWGPMYVAKLKKSWSTGKW

Target ORF information:

RefSeq Version	NM_022072.5
Organism	Homo sapiens(human)
Definition	Homo sapiens NOP2/Sun RNA methyltransferase 3 (NSUN3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022072.5

ORF Insert Sequence:

ATGCTGACCC AGCTGAAAGC AAAATCAGAG GGGAAGCTTG CAAAACAGAT TTGCAAAGTT 
GTGTTGGATC ATTTTGAAAA ACAGTATTCC AAAGAACTCG GAGATGCCTG GAATACAGTA 
AGGGAGATAC TAACATCTCC ATCATGCTGG CAATATGCTG TCCTGCTTAA CCGATTCAAT 
TATCCTTTTG AACTGGAAAA GGATTTACAT TTGAAGGGCT ATCACACACT CTCTCAGGGA 
TCTTTACCCA ACTATCCTAA ATCAGTGAAG TGTTACCTTA GCAGAACTCC GGGCCGAATC 
CCTTCAGAAA GACACCAAAT TGGAAACCTG AAAAAATATT ATCTCCTAAA TGCTGCTTCT 
CTTCTCCCAG TGTTGGCTCT GGAATTAAGG GATGGGGAGA AGGTTCTGGA TCTCTGTGCT 
GCTCCTGGAG GGAAATCAAT AGCTCTGCTG CAGTGTGCTT GTCCAGGTTA TCTTCATTGT 
AATGAATATG ATAGTCTGAG ATTGAGGTGG CTAAGGCAGA CGTTGGAATC TTTCATCCCA 
CAGCCTTTGA TAAATGTAAT TAAAGTGTCT GAATTGGATG GCAGAAAAAT GGGAGATGCC 
CAGCCTGAAA TGTTTGACAA GGTGTTAGTG GATGCTCCGT GTTCAAATGA TCGAAGCTGG 
TTGTTTTCTT CTGACTCTCA GAAGGCATCC TGTAGGATAA GTCAAAGGAG GAATTTGCCT 
CTTCTACAGA TAGAGCTGTT AAGGTCTGCA ATTAAGGCCT TACGTCCTGG AGGGATACTT 
GTATACTCTA CATGCACGCT TTCCAAGGCA GAAAATCAAG ATGTGATCAG TGAAATTTTA 
AACTCCCACG GTAACATCAT GCCTATGGAC ATTAAAGGAA TAGCAAGGAC TTGCTCCCAC 
GACTTCACAT TTGCTCCCAC TGGCCAGGAA TGTGGGCTCT TAGTGATTCC AGATAAGGGC 
AAAGCCTGGG GCCCAATGTA TGTAGCCAAA TTGAAGAAAT CATGGAGCAC AGGAAAATGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NSUN3 ( NM_022072.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_071355.1 Homo sapiens NOP2/Sun RNA methyltransferase family member 3 (NSUN3), mRNA.

Price & Availability↑

CloneID	OHu17445
Clone ID Related Accession (Same CDS sequence)	NM_022072.3 , NM_022072.5 , NM_022072.4
Accession Version	NM_022072.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-14
Organism	Homo sapiens(human)
Product	tRNA (cytosine(34)-C(5))-methyltransferase, mitochondrial
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC130896.3, BU662707.1, BC020602.1, AC019233.7 and BQ030336.1. On Jan 19, 2017 this sequence version replaced NM_022072.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.205930.1, SRR1803615.125322.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGACCC AGCTGAAAGC AAAATCAGAG GGGAAGCTTG CAAAACAGAT TTGCAAAGTT 
GTGTTGGATC ATTTTGAAAA ACAGTATTCC AAAGAACTCG GAGATGCCTG GAATACAGTA 
AGGGAGATAC TAACATCTCC ATCATGCTGG CAATATGCTG TCCTGCTTAA CCGATTCAAT 
TATCCTTTTG AACTGGAAAA GGATTTACAT TTGAAGGGCT ATCACACACT CTCTCAGGGA 
TCTTTACCCA ACTATCCTAA ATCAGTGAAG TGTTACCTTA GCAGAACTCC GGGCCGAATC 
CCTTCAGAAA GACACCAAAT TGGAAACCTG AAAAAATATT ATCTCCTAAA TGCTGCTTCT 
CTTCTCCCAG TGTTGGCTCT GGAATTAAGG GATGGGGAGA AGGTTCTGGA TCTCTGTGCT 
GCTCCTGGAG GGAAATCAAT AGCTCTGCTG CAGTGTGCTT GTCCAGGTTA TCTTCATTGT 
AATGAATATG ATAGTCTGAG ATTGAGGTGG CTAAGGCAGA CGTTGGAATC TTTCATCCCA 
CAGCCTTTGA TAAATGTAAT TAAAGTGTCT GAATTGGATG GCAGAAAAAT GGGAGATGCC 
CAGCCTGAAA TGTTTGACAA GGTGTTAGTG GATGCTCCGT GTTCAAATGA TCGAAGCTGG 
TTGTTTTCTT CTGACTCTCA GAAGGCATCC TGTAGGATAA GTCAAAGGAG GAATTTGCCT 
CTTCTACAGA TAGAGCTGTT AAGGTCTGCA ATTAAGGCCT TACGTCCTGG AGGGATACTT 
GTATACTCTA CATGCACGCT TTCCAAGGCA GAAAATCAAG ATGTGATCAG TGAAATTTTA 
AACTCCCACG GTAACATCAT GCCTATGGAC ATTAAAGGAA TAGCAAGGAC TTGCTCCCAC 
GACTTCACAT TTGCTCCCAC TGGCCAGGAA TGTGGGCTCT TAGTGATTCC AGATAAGGGC 
AAAGCCTGGG GCCCAATGTA TGTAGCCAAA TTGAAGAAAT CATGGAGCAC AGGAAAATGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071355.1
CDS	223..1245
Translation	MLTQLKAKSEGKLAKQICKVVLDHFEKQYSKELGDAWNTVREILTSPSCWQYAVLLNRFNYPFELEKDLHLKGYHTLSQGSLPNYPKSVKCYLSRTPGRIPSERHQIGNLKKYYLLNAASLLPVLALELRDGEKVLDLCAAPGGKSIALLQCACPGYLHCNEYDSLRLRWLRQTLESFIPQPLINVIKVSELDGRKMGDAQPEMFDKVLVDAPCSNDRSWLFSSDSQKASCRISQRRNLPLLQIELLRSAIKALRPGGILVYSTCTLSKAENQDVISEILNSHGNIMPMDIKGIARTCSHDFTFAPTGQECGLLVIPDKGKAWGPMYVAKLKKSWSTGKW

Target ORF information:

RefSeq Version	NM_022072.4
Organism	Homo sapiens(human)
Definition	Homo sapiens NOP2/Sun RNA methyltransferase family member 3 (NSUN3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022072.4

ORF Insert Sequence:

ATGCTGACCC AGCTGAAAGC AAAATCAGAG GGGAAGCTTG CAAAACAGAT TTGCAAAGTT 
GTGTTGGATC ATTTTGAAAA ACAGTATTCC AAAGAACTCG GAGATGCCTG GAATACAGTA 
AGGGAGATAC TAACATCTCC ATCATGCTGG CAATATGCTG TCCTGCTTAA CCGATTCAAT 
TATCCTTTTG AACTGGAAAA GGATTTACAT TTGAAGGGCT ATCACACACT CTCTCAGGGA 
TCTTTACCCA ACTATCCTAA ATCAGTGAAG TGTTACCTTA GCAGAACTCC GGGCCGAATC 
CCTTCAGAAA GACACCAAAT TGGAAACCTG AAAAAATATT ATCTCCTAAA TGCTGCTTCT 
CTTCTCCCAG TGTTGGCTCT GGAATTAAGG GATGGGGAGA AGGTTCTGGA TCTCTGTGCT 
GCTCCTGGAG GGAAATCAAT AGCTCTGCTG CAGTGTGCTT GTCCAGGTTA TCTTCATTGT 
AATGAATATG ATAGTCTGAG ATTGAGGTGG CTAAGGCAGA CGTTGGAATC TTTCATCCCA 
CAGCCTTTGA TAAATGTAAT TAAAGTGTCT GAATTGGATG GCAGAAAAAT GGGAGATGCC 
CAGCCTGAAA TGTTTGACAA GGTGTTAGTG GATGCTCCGT GTTCAAATGA TCGAAGCTGG 
TTGTTTTCTT CTGACTCTCA GAAGGCATCC TGTAGGATAA GTCAAAGGAG GAATTTGCCT 
CTTCTACAGA TAGAGCTGTT AAGGTCTGCA ATTAAGGCCT TACGTCCTGG AGGGATACTT 
GTATACTCTA CATGCACGCT TTCCAAGGCA GAAAATCAAG ATGTGATCAG TGAAATTTTA 
AACTCCCACG GTAACATCAT GCCTATGGAC ATTAAAGGAA TAGCAAGGAC TTGCTCCCAC 
GACTTCACAT TTGCTCCCAC TGGCCAGGAA TGTGGGCTCT TAGTGATTCC AGATAAGGGC 
AAAGCCTGGG GCCCAATGTA TGTAGCCAAA TTGAAGAAAT CATGGAGCAC AGGAAAATGG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NSUN3 ( XM_024453699.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024309467.1 Homo sapiens NOP2/Sun RNA methyltransferase 3 (NSUN3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu71142
Clone ID Related Accession (Same CDS sequence)	XM_024453699.1
Accession Version	XM_024453699.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 615bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tRNA (cytosine(34)-C(5))-methyltransferase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTCCTG TGGGACAGGA AGCTTTTAAG CCAGGACAGT TCCTGCGTCT GCCGCAATGT 
TATCTTCATT GTAATGAATA TGATAGTCTG AGATTGAGGT GGCTAAGGCA GACGTTGGAA 
TCTTTCATCC CACAGCCTTT GATAAATGTA ATTAAAGTGT CTGAATTGGA TGGCAGAAAA 
ATGGGAGATG CCCAGCCTGA AATGTTTGAC AAGGTGTTAG TGGATGCTCC GTGTTCAAAT 
GATCGAAGCT GGTTGTTTTC TTCTGACTCT CAGAAGGCAT CCTGTAGGAT AAGTCAAAGG 
AGGAATTTGC CTCTTCTACA GATAGAGCTG TTAAGGTCTG CAATTAAGGC CTTACGTCCT 
GGAGGGATAC TTGTATACTC TACATGCACG CTTTCCAAGG CAGAAAATCA AGATGTGATC 
AGTGAAATTT TAAACTCCCA CGGTAACATC ATGCCTATGG ACATTAAAGG AATAGCAAGG 
ACTTGCTCCC ACGACTTCAC ATTTGCTCCC ACTGGCCAGG AATGTGGGCT CTTAGTGATT 
CCAGATAAGG GCAAAGCCTG GGGCCCAATG TATGTAGCCA AATTGAAGAA ATCATGGAGC 
ACAGGAAAAT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024309467.1
CDS	3160..3774
Translation	MVPVGQEAFKPGQFLRLPQCYLHCNEYDSLRLRWLRQTLESFIPQPLINVIKVSELDGRKMGDAQPEMFDKVLVDAPCSNDRSWLFSSDSQKASCRISQRRNLPLLQIELLRSAIKALRPGGILVYSTCTLSKAENQDVISEILNSHGNIMPMDIKGIARTCSHDFTFAPTGQECGLLVIPDKGKAWGPMYVAKLKKSWSTGKW

Target ORF information:

RefSeq Version	XM_024453699.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NOP2/Sun RNA methyltransferase 3 (NSUN3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453699.1

ORF Insert Sequence:

ATGGTTCCTG TGGGACAGGA AGCTTTTAAG CCAGGACAGT TCCTGCGTCT GCCGCAATGT 
TATCTTCATT GTAATGAATA TGATAGTCTG AGATTGAGGT GGCTAAGGCA GACGTTGGAA 
TCTTTCATCC CACAGCCTTT GATAAATGTA ATTAAAGTGT CTGAATTGGA TGGCAGAAAA 
ATGGGAGATG CCCAGCCTGA AATGTTTGAC AAGGTGTTAG TGGATGCTCC GTGTTCAAAT 
GATCGAAGCT GGTTGTTTTC TTCTGACTCT CAGAAGGCAT CCTGTAGGAT AAGTCAAAGG 
AGGAATTTGC CTCTTCTACA GATAGAGCTG TTAAGGTCTG CAATTAAGGC CTTACGTCCT 
GGAGGGATAC TTGTATACTC TACATGCACG CTTTCCAAGG CAGAAAATCA AGATGTGATC 
AGTGAAATTT TAAACTCCCA CGGTAACATC ATGCCTATGG ACATTAAAGG AATAGCAAGG 
ACTTGCTCCC ACGACTTCAC ATTTGCTCCC ACTGGCCAGG AATGTGGGCT CTTAGTGATT 
CCAGATAAGG GCAAAGCCTG GGGCCCAATG TATGTAGCCA AATTGAAGAA ATCATGGAGC 
ACAGGAAAAT GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

NSUN3 and ABH1 modify the wobble position of mt-tRNAMet to expand codon recognition in mitochondrial translation.
The EMBO journal35(19)2104-2119(2016 10)
Haag S,Sloan KE,Ranjan N,Warda AS,Kretschmer J,Blessing C,H?bner B,Seikowski J,Dennerlein S,Rehling P,Rodnina MV,H?bartner C,Bohnsack MT

NSUN3 methylase initiates 5-formylcytidine biogenesis in human mitochondrial tRNA(Met).
Nature chemical biology12(7)546-51(2016 07)
Nakano S,Suzuki T,Kawarada L,Iwata H,Asano K,Suzuki T

Deficient methylation and formylation of mt-tRNA(Met) wobble cytosine in a patient carrying mutations in NSUN3.
Nature communications712039(2016 Jun)
Van Haute L,Dietmann S,Kremer L,Hussain S,Pearce SF,Powell CA,Rorbach J,Lantaff R,Blanco S,Sauer S,Kotzaeridou U,Hoffmann GF,Memari Y,Kolb-Kokocinski A,Durbin R,Mayr JA,Frye M,Prokisch H,Minczuk M

Genome-wide association study identifies 8 novel loci associated with blood pressure responses to interventions in Han Chinese.
Circulation. Cardiovascular genetics6(6)598-607(2013 Dec)
He J,Kelly TN,Zhao Q,Li H,Huang J,Wang L,Jaquish CE,Sung YJ,Shimmin LC,Lu F,Mu J,Hu D,Ji X,Shen C,Guo D,Ma J,Wang R,Shen J,Li S,Chen J,Mei H,Chen CS,Chen S,Chen J,Li J,Cao J,Lu X,Wu X,Rice TK,Gu CC,Schwander K,Hamm LL,Liu D,Rao DC,Hixson JE,Gu D

Coeliac disease-associated risk variants in TNFAIP3 and REL implicate altered NF-kappaB signalling.
Gut58(8)1078-83(2009 Aug)
Trynka G,Zhernakova A,Romanos J,Franke L,Hunt KA,Turner G,Bruinenberg M,Heap GA,Platteel M,Ryan AW,de Kovel C,Holmes GK,Howdle PD,Walters JR,Sanders DS,Mulder CJ,Mearin ML,Verbeek WH,Trimble V,Stevens FM,Kelleher D,Barisani D,Bardella MT,McManus R,van Heel DA,Wijmenga C

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NSUN3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

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FAX

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