ABHD4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ABHD4
Entrez Gene ID	63874
Full Name	abhydrolase domain containing 4
Synonyms	ABH4
General protein information	Preferred Names abhydrolase domain containing 4 Names protein ABHD4 abhydrolase domain-containing protein 4 alpha/beta hydrolase domain-containing protein 4 alpha/beta-hydrolase 4 lyso-N-acylphosphatidylethanolamine lipase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q11.2

mRNA and Protein(s)

mRNA	Protein	Name
NM_022060.2	NP_071343.2	(Lyso)-N-acylphosphatidylethanolamine lipase
XM_005267986.4	XP_005268043.1	(Lyso)-N-acylphosphatidylethanolamine lipase isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Abhd4	NP_001102336.1
Oryza sativa (rice)	Os09g0520200	NP_001063697.2
Xenopus tropicalis (tropical clawed frog)	abhd4	NP_001017287.1
Homo sapiens (human)	ABHD4	NP_071343.2
Pan troglodytes (chimpanzee)	ABHD4	XP_509839.3
Drosophila melanogaster (fruit fly)	CG1882	NP_610326.1
Caenorhabditis elegans (roundworm)	C37H5.2	NP_504299.2
Arabidopsis thaliana (thale cress)	AT4G24160	NP_194147.2
Bos taurus (cattle)	ABHD4	NP_001029540.1
Danio rerio (zebrafish)	abhd4	NP_001017613.1
Caenorhabditis elegans (roundworm)	C37H5.3	NP_504297.1
	ABHD4	XP_001099451.1
Canis lupus familiaris (dog)	ABHD4	XP_848701.2
Mus musculus (house mouse)	Abhd4	NP_598837.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000784	XP_311323.4

Gene Ontology
Bibliography

Related articles in PubMed

ABHD4 regulates multiple classes of N-acyl phospholipids in the mammalian central nervous system.
Lee HC, Simon GM, Cravatt BF
Biochemistry54(15)2539-49(2015 Apr)

A genome wide shRNA screen identifies ?/? hydrolase domain containing 4 (ABHD4) as a novel regulator of anoikis resistance.
Simpson CD, Hurren R, Kasimer D, MacLean N, Eberhard Y, Ketela T, Moffat J, Schimmer AD
Apoptosis : an international journal on programmed cell death17(7)666-78(2012 Jul)

Endocannabinoid biosynthesis proceeding through glycerophospho-N-acyl ethanolamine and a role for alpha/beta-hydrolase 4 in this pathway.
Simon GM, Cravatt BF
The Journal of biological chemistry281(36)26465-72(2006 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ABHD4 is a novel genetic regulator of anoikis sensitivity.
Title: A genome wide shRNA screen identifies ?/? hydrolase domain containing 4 (ABHD4) as a novel regulator of anoikis resistance.

Lysophospholipase/phospholipase B (EC 3.1.1.5) specific for N-acyl phosphatidylethanolamine (NAPE) suggesting a role in the biosynthesis of N-acyl ethanolamines including the endocannabinoid anandamide.
Title: Endocannabinoid biosynthesis proceeding through glycerophospho-N-acyl ethanolamine and a role for alpha/beta-hydrolase 4 in this pathway.

The following ABHD4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ABHD4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu30861	NM_022060.2 Latest version!	Homo sapiens abhydrolase domain containing 4 (ABHD4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu47854	XM_005267986.4 Latest version!	Homo sapiens abhydrolase domain containing 4 (ABHD4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ABHD4 ( NM_022060.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_071343.2 Homo sapiens abhydrolase domain containing 4 (ABHD4), mRNA.

Price & Availability↑

CloneID	OHu30861
Clone ID Related Accession (Same CDS sequence)	NM_022060.2
Accession Version	NM_022060.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-06
Organism	Homo sapiens(human)
Product	(Lyso)-N-acylphosphatidylethanolamine lipase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK022878.1, BC024779.1 and AK024790.1. On Jul 27, 2004 this sequence version replaced NM_022060.1. ##Evidence-Data-START## Transcript exon combination :: BC024779.1, AK022878.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END##

ATGGCCGATG ATCTGGAGCA GCAGTCTCAA GGCTGGCTGA GTAGCTGGCT GCCCACGTGG 
CGCCCCACTT CCATGTCTCA GCTGAAGAAT GTGGAAGCCA GGATCCTCCA GTGTCTCCAG 
AATAAGTTCC TGGCCAGATA TGTATCCCTC CCAAACCAGA ATAAGATCTG GACGGTGACT 
GTGAGCCCCG AGCAAAACGA CCGCACCCCC TTGGTGATGG TGCATGGTTT TGGGGGCGGC 
GTGGGTCTCT GGATCCTCAA CATGGACTCA CTGAGTGCCC GCCGCACACT GCACACCTTC 
GATCTGCTTG GCTTCGGGCG AAGCTCAAGG CCAGCATTCC CAAGGGACCC GGAGGGGGCT 
GAGGATGAGT TTGTGACATC GATAGAGACA TGGCGGGAGA CCATGGGGAT CCCCAGCATG 
ATCCTCCTGG GGCACAGTTT GGGAGGATTC CTGGCCACTT CTTACTCAAT CAAGTACCCT 
GATAGAGTTA AACACCTCAT CCTGGTGGAC CCATGGGGCT TTCCCCTCCG ACCAACTAAC 
CCCAGTGAGA TCCGTGCACC CCCAGCCTGG GTCAAAGCCG TGGCATCTGT CCTAGGACGT 
TCCAATCCAT TGGCTGTTCT TCGAGTAGCT GGGCCCTGGG GGCCTGGTCT GGTGCAGCGA 
TTCCGGCCGG ACTTCAAACG CAAGTTTGCA GACTTCTTTG AAGATGATAC CATATCAGAG 
TATATTTACC ACTGCAACGC ACAGAATCCC AGTGGTGAGA CAGCATTCAA AGCCATGATG 
GAGTCCTTTG GCTGGGCCCG GCGCCCTATG CTGGAGCGAA TTCACTTGAT TCGAAAAGAT 
GTGCCTATCA CTATGATCTA CGGGTCCGAC ACCTGGATAG ATACCAGTAC GGGAAAAAAG 
GTGAAGATGC AGCGGCCGGA TTCCTATGTC CGAGACATGG AGATTAAGGG TGCCTCCCAC 
CATGTCTATG CTGACCAGCC ACACATCTTC AATGCTGTGG TGGAGGAGAT CTGCGACTCA 
GTTGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071343.2
CDS	70..1098
Translation	MADDLEQQSQGWLSSWLPTWRPTSMSQLKNVEARILQCLQNKFLARYVSLPNQNKIWTVTVSPEQNDRTPLVMVHGFGGGVGLWILNMDSLSARRTLHTFDLLGFGRSSRPAFPRDPEGAEDEFVTSIETWRETMGIPSMILLGHSLGGFLATSYSIKYPDRVKHLILVDPWGFPLRPTNPSEIRAPPAWVKAVASVLGRSNPLAVLRVAGPWGPGLVQRFRPDFKRKFADFFEDDTISEYIYHCNAQNPSGETAFKAMMESFGWARRPMLERIHLIRKDVPITMIYGSDTWIDTSTGKKVKMQRPDSYVRDMEIKGASHHVYADQPHIFNAVVEEICDSVD

Target ORF information:

RefSeq Version	NM_022060.2
Organism	Homo sapiens(human)
Definition	Homo sapiens abhydrolase domain containing 4 (ABHD4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022060.2

ORF Insert Sequence:

ATGGCCGATG ATCTGGAGCA GCAGTCTCAA GGCTGGCTGA GTAGCTGGCT GCCCACGTGG 
CGCCCCACTT CCATGTCTCA GCTGAAGAAT GTGGAAGCCA GGATCCTCCA GTGTCTCCAG 
AATAAGTTCC TGGCCAGATA TGTATCCCTC CCAAACCAGA ATAAGATCTG GACGGTGACT 
GTGAGCCCCG AGCAAAACGA CCGCACCCCC TTGGTGATGG TGCATGGTTT TGGGGGCGGC 
GTGGGTCTCT GGATCCTCAA CATGGACTCA CTGAGTGCCC GCCGCACACT GCACACCTTC 
GATCTGCTTG GCTTCGGGCG AAGCTCAAGG CCAGCATTCC CAAGGGACCC GGAGGGGGCT 
GAGGATGAGT TTGTGACATC GATAGAGACA TGGCGGGAGA CCATGGGGAT CCCCAGCATG 
ATCCTCCTGG GGCACAGTTT GGGAGGATTC CTGGCCACTT CTTACTCAAT CAAGTACCCT 
GATAGAGTTA AACACCTCAT CCTGGTGGAC CCATGGGGCT TTCCCCTCCG ACCAACTAAC 
CCCAGTGAGA TCCGTGCACC CCCAGCCTGG GTCAAAGCCG TGGCATCTGT CCTAGGACGT 
TCCAATCCAT TGGCTGTTCT TCGAGTAGCT GGGCCCTGGG GGCCTGGTCT GGTGCAGCGA 
TTCCGGCCGG ACTTCAAACG CAAGTTTGCA GACTTCTTTG AAGATGATAC CATATCAGAG 
TATATTTACC ACTGCAACGC ACAGAATCCC AGTGGTGAGA CAGCATTCAA AGCCATGATG 
GAGTCCTTTG GCTGGGCCCG GCGCCCTATG CTGGAGCGAA TTCACTTGAT TCGAAAAGAT 
GTGCCTATCA CTATGATCTA CGGGTCCGAC ACCTGGATAG ATACCAGTAC GGGAAAAAAG 
GTGAAGATGC AGCGGCCGGA TTCCTATGTC CGAGACATGG AGATTAAGGG TGCCTCCCAC 
CATGTCTATG CTGACCAGCC ACACATCTTC AATGCTGTGG TGGAGGAGAT CTGCGACTCA 
GTTGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ABHD4 ( XM_005267986.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005268043.1 Homo sapiens abhydrolase domain containing 4 (ABHD4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu47854
Clone ID Related Accession (Same CDS sequence)	XM_005267986.4
Accession Version	XM_005267986.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	(Lyso)-N-acylphosphatidylethanolamine lipase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005267986.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCAGC TGAAGAATGT GGAAGCCAGG ATCCTCCAGT GTCTCCAGAA TAAGTTCCTG 
GCCAGATATG TATCCCTCCC AAACCAGAAT AAGATCTGGA CGGTGACTGT GAGCCCCGAG 
CAAAACGACC GCACCCCCTT GGTGATGGTG CATGGTTTTG GGGGCGGCGT GGGTCTCTGG 
ATCCTCAACA TGGACTCACT GAGTGCCCGC CGCACACTGC ACACCTTCGA TCTGCTTGGC 
TTCGGGCGAA GCTCAAGGCC AGCATTCCCA AGGGACCCGG AGGGGGCTGA GGATGAGTTT 
GTGACATCGA TAGAGACATG GCGGGAGACC ATGGGGATCC CCAGCATGAT CCTCCTGGGG 
CACAGTTTGG GAGGATTCCT GGCCACTTCT TACTCAATCA AGTACCCTGA TAGAGTTAAA 
CACCTCATCC TGGTGGACCC ATGGGGCTTT CCCCTCCGAC CAACTAACCC CAGTGAGATC 
CGTGCACCCC CAGCCTGGGT CAAAGCCGTG GCATCTGTCC TAGGACGTTC CAATCCATTG 
GCTGTTCTTC GAGTAGCTGG GCCCTGGGGG CCTGGTCTGG TGCAGCGATT CCGGCCGGAC 
TTCAAACGCA AGTTTGCAGA CTTCTTTGAA GATGATACCA TATCAGAGTA TATTTACCAC 
TGCAACGCAC AGAATCCCAG TGGTGAGACA GCATTCAAAG CCATGATGGA GTCCTTTGGC 
TGGGCCCGGC GCCCTATGCT GGAGCGAATT CACTTGATTC GAAAAGATGT GCCTATCACT 
ATGATCTACG GGTCCGACAC CTGGATAGAT ACCAGTACGG GAAAAAAGGT GAAGATGCAG 
CGGCCGGATT CCTATGTCCG AGACATGGAG ATTAAGGGTG CCTCCCACCA TGTCTATGCT 
GACCAGCCAC ACATCTTCAA TGCTGTGGTG GAGGAGATCT GCGACTCAGT TGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005268043.1
CDS	195..1151
Translation	MSQLKNVEARILQCLQNKFLARYVSLPNQNKIWTVTVSPEQNDRTPLVMVHGFGGGVGLWILNMDSLSARRTLHTFDLLGFGRSSRPAFPRDPEGAEDEFVTSIETWRETMGIPSMILLGHSLGGFLATSYSIKYPDRVKHLILVDPWGFPLRPTNPSEIRAPPAWVKAVASVLGRSNPLAVLRVAGPWGPGLVQRFRPDFKRKFADFFEDDTISEYIYHCNAQNPSGETAFKAMMESFGWARRPMLERIHLIRKDVPITMIYGSDTWIDTSTGKKVKMQRPDSYVRDMEIKGASHHVYADQPHIFNAVVEEICDSVD

Target ORF information:

RefSeq Version	XM_005267986.4
Organism	Homo sapiens(human)
Definition	Homo sapiens abhydrolase domain containing 4 (ABHD4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005267986.4

ORF Insert Sequence:

ATGTCTCAGC TGAAGAATGT GGAAGCCAGG ATCCTCCAGT GTCTCCAGAA TAAGTTCCTG 
GCCAGATATG TATCCCTCCC AAACCAGAAT AAGATCTGGA CGGTGACTGT GAGCCCCGAG 
CAAAACGACC GCACCCCCTT GGTGATGGTG CATGGTTTTG GGGGCGGCGT GGGTCTCTGG 
ATCCTCAACA TGGACTCACT GAGTGCCCGC CGCACACTGC ACACCTTCGA TCTGCTTGGC 
TTCGGGCGAA GCTCAAGGCC AGCATTCCCA AGGGACCCGG AGGGGGCTGA GGATGAGTTT 
GTGACATCGA TAGAGACATG GCGGGAGACC ATGGGGATCC CCAGCATGAT CCTCCTGGGG 
CACAGTTTGG GAGGATTCCT GGCCACTTCT TACTCAATCA AGTACCCTGA TAGAGTTAAA 
CACCTCATCC TGGTGGACCC ATGGGGCTTT CCCCTCCGAC CAACTAACCC CAGTGAGATC 
CGTGCACCCC CAGCCTGGGT CAAAGCCGTG GCATCTGTCC TAGGACGTTC CAATCCATTG 
GCTGTTCTTC GAGTAGCTGG GCCCTGGGGG CCTGGTCTGG TGCAGCGATT CCGGCCGGAC 
TTCAAACGCA AGTTTGCAGA CTTCTTTGAA GATGATACCA TATCAGAGTA TATTTACCAC 
TGCAACGCAC AGAATCCCAG TGGTGAGACA GCATTCAAAG CCATGATGGA GTCCTTTGGC 
TGGGCCCGGC GCCCTATGCT GGAGCGAATT CACTTGATTC GAAAAGATGT GCCTATCACT 
ATGATCTACG GGTCCGACAC CTGGATAGAT ACCAGTACGG GAAAAAAGGT GAAGATGCAG 
CGGCCGGATT CCTATGTCCG AGACATGGAG ATTAAGGGTG CCTCCCACCA TGTCTATGCT 
GACCAGCCAC ACATCTTCAA TGCTGTGGTG GAGGAGATCT GCGACTCAGT TGATTGA