DOLPP1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DOLPP1
Entrez Gene ID	57171
Full Name	dolichyldiphosphatase 1
Synonyms	LSFR2
General protein information	Preferred Names dolichyldiphosphatase 1 Names dolichyldiphosphatase 1 dolichyl pyrophosphate phosphatase 1 linked to Surfeit genes in Fugu rubripes 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9q34.11
Summary	A similar gene has been characterized in mice and encodes dolichyl pyrophosphate (Dol-P-P) phosphatase. This protein dephosphorylates dolichyl pyrophosphate so that it may be re-utilized as a glycosyl carrier lipid by the oligosaccharyltransferase multisubunit complex in the ER. Alternative splicing results in multiple transcript variants and protein isoforms. [provided by RefSeq, Jun 2012].
Disorder MIM:	614516

mRNA and Protein(s)

mRNA	Protein	Name
NM_020438.4	NP_065171.2	dolichyldiphosphatase 1 isoform a
NM_001135917.2	NP_001129389.1	dolichyldiphosphatase 1 isoform b
NM_001135917.1	NP_001129389.1	dolichyldiphosphatase 1 isoform b
NM_020438.5	NP_065171.2	dolichyldiphosphatase 1 isoform a

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DOLPP1	XP_520304.2
Danio rerio (zebrafish)	dolpp1	NP_001007062.1
Kluyveromyces lactis	KLLA0B05687g	XP_451789.1
Xenopus tropicalis (tropical clawed frog)	dolpp1	NP_001017014.1
Homo sapiens (human)	DOLPP1	NP_065171.2
Bos taurus (cattle)	DOLPP1	NP_001178970.1
Mus musculus (house mouse)	Dolpp1	NP_065062.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002666	XP_312258.3
Saccharomyces cerevisiae (baker's yeast)	CAX4	NP_011550.3
Magnaporthe oryzae (rice blast fungus)	MGG_17385	XP_003718278.1
Canis lupus familiaris (dog)	DOLPP1	XP_850246.1
Neurospora crassa	NCU03718	XP_961494.1
Arabidopsis thaliana (thale cress)	AT5G03080	NP_195928.1
Oryza sativa (rice)	Os03g0288700	NP_001049787.1
Rattus norvegicus (Norway rat)	Dolpp1	NP_001100037.1
Gallus gallus (chicken)	DOLPP1	NP_001025860.2
Eremothecium gossypii	AGOS_ACL186W	NP_983218.1

Gene Ontology
Bibliography

Related articles in PubMed

Prominent use of distal 5' transcription start sites and discovery of a large number of additional exons in ENCODE regions.
Denoeud F, Kapranov P, Ucla C, Frankish A, Castelo R, Drenkow J, Lagarde J, Alioto T, Manzano C, Chrast J, Dike S, Wyss C, Henrichsen CN, Holroyd N, Dickson MC, Taylor R, Hance Z, Foissac S, Myers RM, Rogers J, Hubbard T, Harrow J, Guig? R, Gingeras TR, Antonarakis SE, Reymond A
Genome research17(6)746-59(2007 Jun)

Identification and characterization of a cDNA encoding a dolichyl pyrophosphate phosphatase located in the endoplasmic reticulum of mammalian cells.
Rush JS, Cho SK, Jiang S, Hofmann SL, Waechter CJ
The Journal of biological chemistry277(47)45226-34(2002 Nov)

Extensive gene order differences within regions of conserved synteny between the Fugu and human genomes: implications for chromosomal evolution and the cloning of disease genes.
Gilley J, Fried M
Human molecular genetics8(7)1313-20(1999 Jul)

Enzymatic activities in cultured human lymphocytes that dephosphorylate dolichyl pyrophosphate and dolichyl phosphate.
Wedgwood JF, Strominger JL
The Journal of biological chemistry255(3)1120-3(1980 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DOLPP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DOLPP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu03344	NM_020438.5 Latest version!	Homo sapiens dolichyldiphosphatase 1 (DOLPP1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu30581	NM_001135917.2 Latest version!	Homo sapiens dolichyldiphosphatase 1 (DOLPP1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu30581	NM_001135917.1	Homo sapiens dolichyldiphosphatase 1 (DOLPP1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu03344	NM_020438.4	Homo sapiens dolichyldiphosphatase 1 (DOLPP1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DOLPP1 ( NM_020438.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_065171.2 Homo sapiens dolichyldiphosphatase 1 (DOLPP1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu03344
Clone ID Related Accession (Same CDS sequence)	NM_020438.4 , NM_020438.5
Accession Version	NM_020438.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	dolichyldiphosphatase 1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA590931.1, BC009493.2 and BC033686.1. On Oct 17, 2008 this sequence version replaced NM_020438.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). ##Evidence-Data-START## Transcript exon combination :: BC033686.1, SRR1163655.360410.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGCGG ACGGACAGTG CTCGCTCCCC GCTTCATGGC GGCCGGTGAC CCTCACCCAC 
GTCGAATATC CTGCAGGTGA TCTCTCTGGC CACCTCCTTG CCTACCTGAG CCTCAGCCCT 
GTATTTGTCA TCGTCGGTTT CGTGACCCTC ATCATATTTA AGCGGGAGCT GCACACGATC 
TCCTTCCTTG GGGGCCTGGC ACTGAACGAG GGGGTCAACT GGCTGATCAA AAACGTCATC 
CAGGAGCCAC GGCCCTGTGG AGGCCCCCAC ACAGCAGTGG GCACCAAGTA CGGGATGCCC 
TCCAGCCATT CCCAGTTTAT GTGGTTCTTC TCCGTCTATT CCTTCCTTTT CCTGTATTTA 
AGAATGCACC AAACAAACAA CGCCAGGTTC CTGGACTTGC TGTGGAGGCA CGTGCTCTCC 
CTGGGACTCC TCGCTGTGGC CTTCCTAGTC TCCTACAGCA GGGTCTACCT GCTGTACCAC 
ACCTGGAGCC AGGTGCTCTA TGGAGGCATC GCTGGAGGCC TCATGGCCAT CGCCTGGTTC 
ATCTTCACCC AGGAGGTCCT CACCCCGCTG TTCCCCAGGA TAGCAGCCTG GCCTGTCTCC 
GAGTTCTTCC TAATCCGAGA CACAAGCCTC ATTCCCAACG TACTCTGGTT TGAGTACACG 
GTAACCCGGG CAGAAGCCAG GAACAGACAA CGCAAGCTGG GGACGAAACT GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065171.2
CDS	29..745
Translation	MAADGQCSLPASWRPVTLTHVEYPAGDLSGHLLAYLSLSPVFVIVGFVTLIIFKRELHTISFLGGLALNEGVNWLIKNVIQEPRPCGGPHTAVGTKYGMPSSHSQFMWFFSVYSFLFLYLRMHQTNNARFLDLLWRHVLSLGLLAVAFLVSYSRVYLLYHTWSQVLYGGIAGGLMAIAWFIFTQEVLTPLFPRIAAWPVSEFFLIRDTSLIPNVLWFEYTVTRAEARNRQRKLGTKLQ

Target ORF information:

RefSeq Version	NM_020438.4
Organism	Homo sapiens(human)
Definition	Homo sapiens dolichyldiphosphatase 1 (DOLPP1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020438.4

ORF Insert Sequence:

ATGGCAGCGG ACGGACAGTG CTCGCTCCCC GCTTCATGGC GGCCGGTGAC CCTCACCCAC 
GTCGAATATC CTGCAGGTGA TCTCTCTGGC CACCTCCTTG CCTACCTGAG CCTCAGCCCT 
GTATTTGTCA TCGTCGGTTT CGTGACCCTC ATCATATTTA AGCGGGAGCT GCACACGATC 
TCCTTCCTTG GGGGCCTGGC ACTGAACGAG GGGGTCAACT GGCTGATCAA AAACGTCATC 
CAGGAGCCAC GGCCCTGTGG AGGCCCCCAC ACAGCAGTGG GCACCAAGTA CGGGATGCCC 
TCCAGCCATT CCCAGTTTAT GTGGTTCTTC TCCGTCTATT CCTTCCTTTT CCTGTATTTA 
AGAATGCACC AAACAAACAA CGCCAGGTTC CTGGACTTGC TGTGGAGGCA CGTGCTCTCC 
CTGGGACTCC TCGCTGTGGC CTTCCTAGTC TCCTACAGCA GGGTCTACCT GCTGTACCAC 
ACCTGGAGCC AGGTGCTCTA TGGAGGCATC GCTGGAGGCC TCATGGCCAT CGCCTGGTTC 
ATCTTCACCC AGGAGGTCCT CACCCCGCTG TTCCCCAGGA TAGCAGCCTG GCCTGTCTCC 
GAGTTCTTCC TAATCCGAGA CACAAGCCTC ATTCCCAACG TACTCTGGTT TGAGTACACG 
GTAACCCGGG CAGAAGCCAG GAACAGACAA CGCAAGCTGG GGACGAAACT GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOLPP1 ( NM_020438.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_065171.2 Homo sapiens dolichyldiphosphatase 1 (DOLPP1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu03344
Clone ID Related Accession (Same CDS sequence)	NM_020438.4 , NM_020438.5
Accession Version	NM_020438.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	dolichyldiphosphatase 1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA590931.1, BC009493.2 and BC033686.1. On Nov 23, 2018 this sequence version replaced NM_020438.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). ##Evidence-Data-START## Transcript exon combination :: SRR3476690.8411.1, SRR3476690.116041.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000372546.9/ ENSP00000361625.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCAGCGG ACGGACAGTG CTCGCTCCCC GCTTCATGGC GGCCGGTGAC CCTCACCCAC 
GTCGAATATC CTGCAGGTGA TCTCTCTGGC CACCTCCTTG CCTACCTGAG CCTCAGCCCT 
GTATTTGTCA TCGTCGGTTT CGTGACCCTC ATCATATTTA AGCGGGAGCT GCACACGATC 
TCCTTCCTTG GGGGCCTGGC ACTGAACGAG GGGGTCAACT GGCTGATCAA AAACGTCATC 
CAGGAGCCAC GGCCCTGTGG AGGCCCCCAC ACAGCAGTGG GCACCAAGTA CGGGATGCCC 
TCCAGCCATT CCCAGTTTAT GTGGTTCTTC TCCGTCTATT CCTTCCTTTT CCTGTATTTA 
AGAATGCACC AAACAAACAA CGCCAGGTTC CTGGACTTGC TGTGGAGGCA CGTGCTCTCC 
CTGGGACTCC TCGCTGTGGC CTTCCTAGTC TCCTACAGCA GGGTCTACCT GCTGTACCAC 
ACCTGGAGCC AGGTGCTCTA TGGAGGCATC GCTGGAGGCC TCATGGCCAT CGCCTGGTTC 
ATCTTCACCC AGGAGGTCCT CACCCCGCTG TTCCCCAGGA TAGCAGCCTG GCCTGTCTCC 
GAGTTCTTCC TAATCCGAGA CACAAGCCTC ATTCCCAACG TACTCTGGTT TGAGTACACG 
GTAACCCGGG CAGAAGCCAG GAACAGACAA CGCAAGCTGG GGACGAAACT GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065171.2
CDS	22..738
Translation	MAADGQCSLPASWRPVTLTHVEYPAGDLSGHLLAYLSLSPVFVIVGFVTLIIFKRELHTISFLGGLALNEGVNWLIKNVIQEPRPCGGPHTAVGTKYGMPSSHSQFMWFFSVYSFLFLYLRMHQTNNARFLDLLWRHVLSLGLLAVAFLVSYSRVYLLYHTWSQVLYGGIAGGLMAIAWFIFTQEVLTPLFPRIAAWPVSEFFLIRDTSLIPNVLWFEYTVTRAEARNRQRKLGTKLQ

Target ORF information:

RefSeq Version	NM_020438.5
Organism	Homo sapiens(human)
Definition	Homo sapiens dolichyldiphosphatase 1 (DOLPP1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020438.5

ORF Insert Sequence:

ATGGCAGCGG ACGGACAGTG CTCGCTCCCC GCTTCATGGC GGCCGGTGAC CCTCACCCAC 
GTCGAATATC CTGCAGGTGA TCTCTCTGGC CACCTCCTTG CCTACCTGAG CCTCAGCCCT 
GTATTTGTCA TCGTCGGTTT CGTGACCCTC ATCATATTTA AGCGGGAGCT GCACACGATC 
TCCTTCCTTG GGGGCCTGGC ACTGAACGAG GGGGTCAACT GGCTGATCAA AAACGTCATC 
CAGGAGCCAC GGCCCTGTGG AGGCCCCCAC ACAGCAGTGG GCACCAAGTA CGGGATGCCC 
TCCAGCCATT CCCAGTTTAT GTGGTTCTTC TCCGTCTATT CCTTCCTTTT CCTGTATTTA 
AGAATGCACC AAACAAACAA CGCCAGGTTC CTGGACTTGC TGTGGAGGCA CGTGCTCTCC 
CTGGGACTCC TCGCTGTGGC CTTCCTAGTC TCCTACAGCA GGGTCTACCT GCTGTACCAC 
ACCTGGAGCC AGGTGCTCTA TGGAGGCATC GCTGGAGGCC TCATGGCCAT CGCCTGGTTC 
ATCTTCACCC AGGAGGTCCT CACCCCGCTG TTCCCCAGGA TAGCAGCCTG GCCTGTCTCC 
GAGTTCTTCC TAATCCGAGA CACAAGCCTC ATTCCCAACG TACTCTGGTT TGAGTACACG 
GTAACCCGGG CAGAAGCCAG GAACAGACAA CGCAAGCTGG GGACGAAACT GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOLPP1 ( NM_001135917.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001129389.1 Homo sapiens dolichyldiphosphatase 1 (DOLPP1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu30581
Clone ID Related Accession (Same CDS sequence)	NM_001135917.2 , NM_001135917.1
Accession Version	NM_001135917.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	dolichyldiphosphatase 1 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA590931.1, BC009493.2 and BC033686.1. On May 31, 2019 this sequence version replaced NM_001135917.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the coding region compared to variant 1. It encodes isoform b which is shorter compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: BC067858.1, SRR3476690.929473.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGCGG ACGGACAGTG CTCGCTCCCC GCTTCATGGC GGCCGGTGAC CCTCACCCAC 
GTCGAATATC CTGCAGGTGA TCTCTCTGGC CACCTCCTTG CCTACCTGAG CCTCAGCCCT 
GTATTTGTCA TCGTCGGTTT CGTGACCCTC ATCATATTTA AGCGGGAGCT GCACACGATC 
TCCTTCCTTG GGGGCCTGGC ACTGAACGAG GGGGTCAACT GGCTGATCAA AAACGTCATC 
CAGGAGCCAC GGCCCTGTGG AGGCCCCCAC ACAGCAGTGG GCACCAAGTA CGGGATGCCC 
TCCAGCCATT CCCAGTTTAT GTGGTTCTTC TCCGTCTATT CCTTCCTTTT CCTGTATTTA 
AGAATGCACC AAACAAACAA CGCCAGGTTC CTGGACTTGC TGTGGAGGCA CGTGCTCTCC 
CTGGGACTCC TCGCTGTGGC CTTCCTAGTC TCCTACAGCA GGCCTGTCTC CGAGTTCTTC 
CTAATCCGAG ACACAAGCCT CATTCCCAAC GTACTCTGGT TTGAGTACAC GGTAACCCGG 
GCAGAAGCCA GGAACAGACA ACGCAAGCTG GGGACGAAAC TGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129389.1
CDS	22..609
Translation	MAADGQCSLPASWRPVTLTHVEYPAGDLSGHLLAYLSLSPVFVIVGFVTLIIFKRELHTISFLGGLALNEGVNWLIKNVIQEPRPCGGPHTAVGTKYGMPSSHSQFMWFFSVYSFLFLYLRMHQTNNARFLDLLWRHVLSLGLLAVAFLVSYSRPVSEFFLIRDTSLIPNVLWFEYTVTRAEARNRQRKLGTKLQ

Target ORF information:

RefSeq Version	NM_001135917.2
Organism	Homo sapiens(human)
Definition	Homo sapiens dolichyldiphosphatase 1 (DOLPP1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135917.2

ORF Insert Sequence:

ATGGCAGCGG ACGGACAGTG CTCGCTCCCC GCTTCATGGC GGCCGGTGAC CCTCACCCAC 
GTCGAATATC CTGCAGGTGA TCTCTCTGGC CACCTCCTTG CCTACCTGAG CCTCAGCCCT 
GTATTTGTCA TCGTCGGTTT CGTGACCCTC ATCATATTTA AGCGGGAGCT GCACACGATC 
TCCTTCCTTG GGGGCCTGGC ACTGAACGAG GGGGTCAACT GGCTGATCAA AAACGTCATC 
CAGGAGCCAC GGCCCTGTGG AGGCCCCCAC ACAGCAGTGG GCACCAAGTA CGGGATGCCC 
TCCAGCCATT CCCAGTTTAT GTGGTTCTTC TCCGTCTATT CCTTCCTTTT CCTGTATTTA 
AGAATGCACC AAACAAACAA CGCCAGGTTC CTGGACTTGC TGTGGAGGCA CGTGCTCTCC 
CTGGGACTCC TCGCTGTGGC CTTCCTAGTC TCCTACAGCA GGCCTGTCTC CGAGTTCTTC 
CTAATCCGAG ACACAAGCCT CATTCCCAAC GTACTCTGGT TTGAGTACAC GGTAACCCGG 
GCAGAAGCCA GGAACAGACA ACGCAAGCTG GGGACGAAAC TGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DOLPP1 ( NM_001135917.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001129389.1 Homo sapiens dolichyldiphosphatase 1 (DOLPP1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu30581
Clone ID Related Accession (Same CDS sequence)	NM_001135917.2 , NM_001135917.1
Accession Version	NM_001135917.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	dolichyldiphosphatase 1 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA590931.1, BC009493.2 and BC033686.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the coding region compared to variant 1. It encodes isoform b which is shorter compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: BC067858.1, SRR1803615.75198.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGCGG ACGGACAGTG CTCGCTCCCC GCTTCATGGC GGCCGGTGAC CCTCACCCAC 
GTCGAATATC CTGCAGGTGA TCTCTCTGGC CACCTCCTTG CCTACCTGAG CCTCAGCCCT 
GTATTTGTCA TCGTCGGTTT CGTGACCCTC ATCATATTTA AGCGGGAGCT GCACACGATC 
TCCTTCCTTG GGGGCCTGGC ACTGAACGAG GGGGTCAACT GGCTGATCAA AAACGTCATC 
CAGGAGCCAC GGCCCTGTGG AGGCCCCCAC ACAGCAGTGG GCACCAAGTA CGGGATGCCC 
TCCAGCCATT CCCAGTTTAT GTGGTTCTTC TCCGTCTATT CCTTCCTTTT CCTGTATTTA 
AGAATGCACC AAACAAACAA CGCCAGGTTC CTGGACTTGC TGTGGAGGCA CGTGCTCTCC 
CTGGGACTCC TCGCTGTGGC CTTCCTAGTC TCCTACAGCA GGCCTGTCTC CGAGTTCTTC 
CTAATCCGAG ACACAAGCCT CATTCCCAAC GTACTCTGGT TTGAGTACAC GGTAACCCGG 
GCAGAAGCCA GGAACAGACA ACGCAAGCTG GGGACGAAAC TGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129389.1
CDS	29..616
Translation	MAADGQCSLPASWRPVTLTHVEYPAGDLSGHLLAYLSLSPVFVIVGFVTLIIFKRELHTISFLGGLALNEGVNWLIKNVIQEPRPCGGPHTAVGTKYGMPSSHSQFMWFFSVYSFLFLYLRMHQTNNARFLDLLWRHVLSLGLLAVAFLVSYSRPVSEFFLIRDTSLIPNVLWFEYTVTRAEARNRQRKLGTKLQ

Target ORF information:

RefSeq Version	NM_001135917.1
Organism	Homo sapiens(human)
Definition	Homo sapiens dolichyldiphosphatase 1 (DOLPP1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135917.1

ORF Insert Sequence:

ATGGCAGCGG ACGGACAGTG CTCGCTCCCC GCTTCATGGC GGCCGGTGAC CCTCACCCAC 
GTCGAATATC CTGCAGGTGA TCTCTCTGGC CACCTCCTTG CCTACCTGAG CCTCAGCCCT 
GTATTTGTCA TCGTCGGTTT CGTGACCCTC ATCATATTTA AGCGGGAGCT GCACACGATC 
TCCTTCCTTG GGGGCCTGGC ACTGAACGAG GGGGTCAACT GGCTGATCAA AAACGTCATC 
CAGGAGCCAC GGCCCTGTGG AGGCCCCCAC ACAGCAGTGG GCACCAAGTA CGGGATGCCC 
TCCAGCCATT CCCAGTTTAT GTGGTTCTTC TCCGTCTATT CCTTCCTTTT CCTGTATTTA 
AGAATGCACC AAACAAACAA CGCCAGGTTC CTGGACTTGC TGTGGAGGCA CGTGCTCTCC 
CTGGGACTCC TCGCTGTGGC CTTCCTAGTC TCCTACAGCA GGCCTGTCTC CGAGTTCTTC 
CTAATCCGAG ACACAAGCCT CATTCCCAAC GTACTCTGGT TTGAGTACAC GGTAACCCGG 
GCAGAAGCCA GGAACAGACA ACGCAAGCTG GGGACGAAAC TGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Prominent use of distal 5' transcription start sites and discovery of a large number of additional exons in ENCODE regions.
Genome research17(6)746-59(2007 Jun)
Denoeud F,Kapranov P,Ucla C,Frankish A,Castelo R,Drenkow J,Lagarde J,Alioto T,Manzano C,Chrast J,Dike S,Wyss C,Henrichsen CN,Holroyd N,Dickson MC,Taylor R,Hance Z,Foissac S,Myers RM,Rogers J,Hubbard T,Harrow J,Guig? R,Gingeras TR,Antonarakis SE,Reymond A

Identification and characterization of a cDNA encoding a dolichyl pyrophosphate phosphatase located in the endoplasmic reticulum of mammalian cells.
The Journal of biological chemistry277(47)45226-34(2002 Nov)
Rush JS,Cho SK,Jiang S,Hofmann SL,Waechter CJ

Extensive gene order differences within regions of conserved synteny between the Fugu and human genomes: implications for chromosomal evolution and the cloning of disease genes.
Human molecular genetics8(7)1313-20(1999 Jul)
Gilley J,Fried M

Enzymatic activities in cultured human lymphocytes that dephosphorylate dolichyl pyrophosphate and dolichyl phosphate.
The Journal of biological chemistry255(3)1120-3(1980 Feb)
Wedgwood JF,Strominger JL