PSPN cDNA ORF clone, Homo sapiens(Human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PSPN
Entrez Gene ID	5623
Full Name	persephin
Synonyms	PSP
General protein information	Preferred Names persephin Names persephin
Gene Type	protein-coding
Organism	Homo sapiens(Human)
Genome	Chromosome: 19 Map Location: 19p13.3
Summary	This gene encodes a secreted ligand of the GDNF (glial cell line-derived neurotrophic factor) subfamily and TGF-beta (transforming growth factor-beta) superfamily of proteins. The encoded preproprotein is proteolytically processed to generate the mature protein. This protein signals through the RET receptor tyrosine kinase and a GPI-linked coreceptor, and promotes survival of neuronal populations. This protein may play a role in cell death, and nervous system development and function. Elevated expression of this gene has been observed in oral squamous cell carcinoma. [provided by RefSeq, Aug 2016].
Disorder MIM:	602921

mRNA and Protein(s)

mRNA	Protein	Name
NM_004158.3	NP_004149.1	persephin preproprotein
NM_004158.2	NP_004149.1	persephin precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PSPN	XP_002688959.2
Mus musculus (house mouse)	Pspn	NP_032980.2
Canis lupus familiaris (dog)	PSPN	XP_005633047.1
Rattus norvegicus (Norway rat)	Pspn	NP_037146.1
Homo sapiens (human)	PSPN	NP_004149.1
Pan troglodytes (chimpanzee)	PSPN	XP_512313.3
	PSPN	XP_001087562.2

Gene Ontology
Bibliography

Related articles in PubMed

Persephin: A potential key component in human oral cancer progression through the RET receptor tyrosine kinase-mitogen-activated protein kinase signaling pathway.
Baba T, Sakamoto Y, Kasamatsu A, Minakawa Y, Yokota S, Higo M, Yokoe H, Ogawara K, Shiiba M, Tanzawa H, Uzawa K
Molecular carcinogenesis54(8)608-17(2015 Aug)

Persephin signaling through GFRalpha1: the potential for the treatment of Parkinson's disease.
Sidorova YA, M?tlik K, Paveliev M, Lindahl M, Piranen E, Milbrandt J, Arum?e U, Saarma M, Bespalov MM
Molecular and cellular neurosciences44(3)223-32(2010 Jul)

Polymorphisms in the genes encoding the 4 RET ligands, GDNF, NTN, ARTN, PSPN, and susceptibility to Hirschsprung disease.
Fernandez RM, Ruiz-Ferrer M, Lopez-Alonso M, Anti?olo G, Borrego S
Journal of pediatric surgery43(11)2042-7(2008 Nov)

Neurturin, persephin, and artemin in the human pre- and full-term newborn and adult hippocampus and fascia dentata.
Quartu M, Serra MP, Manca A, Mascia F, Follesa P, Del Fiacco M
Brain research1041(2)157-66(2005 Apr)

PSPN/GFRalpha4 has a significantly weaker capacity than GDNF/GFRalpha1 to recruit RET to rafts, but promotes neuronal survival and neurite outgrowth.
Yang J, Lindahl M, Lindholm P, Virtanen H, Coffey E, Runeberg-Roos P, Saarma M
FEBS letters569(1-3)267-71(2004 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results suggested that PSPN is a possible key regulator of oral squamous cell carcinoma (OSCC) progression via PSPN-RET-mitogen-activated protein kinase activation and that PSPN overexpression may have diagnostic potential for OSCC.
Title: Persephin: A potential key component in human oral cancer progression through the RET receptor tyrosine kinase-mitogen-activated protein kinase signaling pathway.

Results identify persephin, a GDNF family member, as a novel ligand for GFRalpha1/RET receptor complex.
Title: Persephin signaling through GFRalpha1: the potential for the treatment of Parkinson's disease.

Observational study of gene-disease association. (HuGE Navigator)
Title: Polymorphisms in the genes encoding the 4 RET ligands, GDNF, NTN, ARTN, PSPN, and susceptibility to Hirschsprung disease.

The results obtained suggest the involvement of NTN, PSP, and ART in processes subserving both the organization of this cortical region during development and the functional activity and maintenance of the mature human hippocampal neurons.
Title: Neurturin, persephin, and artemin in the human pre- and full-term newborn and adult hippocampus and fascia dentata.

Persephin/GFRalpha4 is unable to recruit RET protein into lipid rafts.
Title: PSPN/GFRalpha4 has a significantly weaker capacity than GDNF/GFRalpha1 to recruit RET to rafts, but promotes neuronal survival and neurite outgrowth.

The following PSPN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PSPN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu04467	NM_004158.3 Latest version!	Homo sapiens persephin (PSPN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu04467	NM_004158.2	Homo sapiens persephin (PSPN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PSPN ( NM_004158.3 ) cDNA ORF clone, Homo sapiens(Human) -> NP_004149.1 Homo sapiens persephin (PSPN), mRNA.

Price & Availability↑

CloneID	OHu04467
Clone ID Related Accession (Same CDS sequence)	NM_004158.3 , NM_004158.2
Accession Version	NM_004158.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Homo sapiens(human)
Product	persephin preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC011491.7, AF040962.1 and BU609071.1. On Aug 4, 2016 this sequence version replaced NM_004158.2. ##Evidence-Data-START## Transcript exon combination :: AF040962.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCGTAG GGAAGTTCCT GCTGGGCTCC CTGCTGCTCC TGTCCCTGCA GCTGGGACAG 
GGCTGGGGCC CCGATGCCCG TGGGGTTCCC GTGGCCGATG GAGAGTTCTC GTCTGAACAG 
GTGGCAAAGG CTGGAGGGAC CTGGCTGGGC ACCCACCGCC CCCTTGCCCG CCTGCGCCGA 
GCCCTGTCTG GTCCATGCCA GCTGTGGAGC CTGACCCTGT CCGTGGCAGA GCTAGGCCTG 
GGCTACGCCT CAGAGGAGAA GGTCATCTTC CGCTACTGCG CCGGCAGCTG CCCCCGTGGT 
GCCCGCACCC AGCATGGCCT GGCGCTGGCC CGGCTGCAGG GCCAGGGCCG AGCCCACGGC 
GGGCCCTGCT GCCGGCCCAC TCGCTACACC GACGTGGCCT TCCTCGATGA CCGCCACCGC 
TGGCAGCGGC TGCCCCAGCT CTCGGCGGCT GCCTGCGGCT GTGGTGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004149.1
CDS	68..538
Translation	MAVGKFLLGSLLLLSLQLGQGWGPDARGVPVADGEFSSEQVAKAGGTWLGTHRPLARLRRALSGPCQLWSLTLSVAELGLGYASEEKVIFRYCAGSCPRGARTQHGLALARLQGQGRAHGGPCCRPTRYTDVAFLDDRHRWQRLPQLSAAACGCGG

Target ORF information:

RefSeq Version	NM_004158.3
Organism	Homo sapiens(human)
Definition	Homo sapiens persephin (PSPN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004158.3

ORF Insert Sequence:

ATGGCCGTAG GGAAGTTCCT GCTGGGCTCC CTGCTGCTCC TGTCCCTGCA GCTGGGACAG 
GGCTGGGGCC CCGATGCCCG TGGGGTTCCC GTGGCCGATG GAGAGTTCTC GTCTGAACAG 
GTGGCAAAGG CTGGAGGGAC CTGGCTGGGC ACCCACCGCC CCCTTGCCCG CCTGCGCCGA 
GCCCTGTCTG GTCCATGCCA GCTGTGGAGC CTGACCCTGT CCGTGGCAGA GCTAGGCCTG 
GGCTACGCCT CAGAGGAGAA GGTCATCTTC CGCTACTGCG CCGGCAGCTG CCCCCGTGGT 
GCCCGCACCC AGCATGGCCT GGCGCTGGCC CGGCTGCAGG GCCAGGGCCG AGCCCACGGC 
GGGCCCTGCT GCCGGCCCAC TCGCTACACC GACGTGGCCT TCCTCGATGA CCGCCACCGC 
TGGCAGCGGC TGCCCCAGCT CTCGGCGGCT GCCTGCGGCT GTGGTGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PSPN ( NM_004158.2 ) cDNA ORF clone, Homo sapiens(Human) -> NP_004149.1 Homo sapiens persephin (PSPN), mRNA.

Price & Availability↑

CloneID	OHu04467
Clone ID Related Accession (Same CDS sequence)	NM_004158.3 , NM_004158.2
Accession Version	NM_004158.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	persephin precursor
Comment

ATGGCCGTAG GGAAGTTCCT GCTGGGCTCC CTGCTGCTCC TGTCCCTGCA GCTGGGACAG 
GGCTGGGGCC CCGATGCCCG TGGGGTTCCC GTGGCCGATG GAGAGTTCTC GTCTGAACAG 
GTGGCAAAGG CTGGAGGGAC CTGGCTGGGC ACCCACCGCC CCCTTGCCCG CCTGCGCCGA 
GCCCTGTCTG GTCCATGCCA GCTGTGGAGC CTGACCCTGT CCGTGGCAGA GCTAGGCCTG 
GGCTACGCCT CAGAGGAGAA GGTCATCTTC CGCTACTGCG CCGGCAGCTG CCCCCGTGGT 
GCCCGCACCC AGCATGGCCT GGCGCTGGCC CGGCTGCAGG GCCAGGGCCG AGCCCACGGC 
GGGCCCTGCT GCCGGCCCAC TCGCTACACC GACGTGGCCT TCCTCGATGA CCGCCACCGC 
TGGCAGCGGC TGCCCCAGCT CTCGGCGGCT GCCTGCGGCT GTGGTGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004149.1
Translation	MAVGKFLLGSLLLLSLQLGQGWGPDARGVPVADGEFSSEQVAKAGGTWLGTHRPLARLRRALSGPCQLWSLTLSVAELGLGYASEEKVIFRYCAGSCPRGARTQHGLALARLQGQGRAHGGPCCRPTRYTDVAFLDDRHRWQRLPQLSAAACGCGG

Target ORF information:

RefSeq Version	NM_004158.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens persephin (PSPN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004158.2

ORF Insert Sequence:

ATGGCCGTAG GGAAGTTCCT GCTGGGCTCC CTGCTGCTCC TGTCCCTGCA GCTGGGACAG 
GGCTGGGGCC CCGATGCCCG TGGGGTTCCC GTGGCCGATG GAGAGTTCTC GTCTGAACAG 
GTGGCAAAGG CTGGAGGGAC CTGGCTGGGC ACCCACCGCC CCCTTGCCCG CCTGCGCCGA 
GCCCTGTCTG GTCCATGCCA GCTGTGGAGC CTGACCCTGT CCGTGGCAGA GCTAGGCCTG 
GGCTACGCCT CAGAGGAGAA GGTCATCTTC CGCTACTGCG CCGGCAGCTG CCCCCGTGGT 
GCCCGCACCC AGCATGGCCT GGCGCTGGCC CGGCTGCAGG GCCAGGGCCG AGCCCACGGC 
GGGCCCTGCT GCCGGCCCAC TCGCTACACC GACGTGGCCT TCCTCGATGA CCGCCACCGC 
TGGCAGCGGC TGCCCCAGCT CTCGGCGGCT GCCTGCGGCT GTGGTGGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Persephin: A potential key component in human oral cancer progression through the RET receptor tyrosine kinase-mitogen-activated protein kinase signaling pathway.
Molecular carcinogenesis54(8)608-17(2015 Aug)
Baba T,Sakamoto Y,Kasamatsu A,Minakawa Y,Yokota S,Higo M,Yokoe H,Ogawara K,Shiiba M,Tanzawa H,Uzawa K

Persephin signaling through GFRalpha1: the potential for the treatment of Parkinson's disease.
Molecular and cellular neurosciences44(3)223-32(2010 Jul)
Sidorova YA,M?tlik K,Paveliev M,Lindahl M,Piranen E,Milbrandt J,Arum?e U,Saarma M,Bespalov MM

Polymorphisms in the genes encoding the 4 RET ligands, GDNF, NTN, ARTN, PSPN, and susceptibility to Hirschsprung disease.
Journal of pediatric surgery43(11)2042-7(2008 Nov)
Fernandez RM,Ruiz-Ferrer M,Lopez-Alonso M,Anti?olo G,Borrego S

Neurturin, persephin, and artemin in the human pre- and full-term newborn and adult hippocampus and fascia dentata.
Brain research1041(2)157-66(2005 Apr)
Quartu M,Serra MP,Manca A,Mascia F,Follesa P,Del Fiacco M

PSPN/GFRalpha4 has a significantly weaker capacity than GDNF/GFRalpha1 to recruit RET to rafts, but promotes neuronal survival and neurite outgrowth.
FEBS letters569(1-3)267-71(2004 Jul)
Yang J,Lindahl M,Lindholm P,Virtanen H,Coffey E,Runeberg-Roos P,Saarma M