ARL8B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ARL8B
Entrez Gene ID	55207
Full Name	ADP ribosylation factor like GTPase 8B
Synonyms	ARL10C,Gie1
General protein information	Preferred Names ADP ribosylation factor like GTPase 8B Names ADP-ribosylation factor-like protein 8B ADP-ribosylation factor-like 10C ADP-ribosylation factor-like 8B ADP-ribosylation factor-like protein 10C novel small G protein indispensable for equal chromosome segregation 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p26.1
Disorder MIM:	616596

mRNA and Protein(s)

mRNA	Protein	Name
NM_018184.2	NP_060654.1	ADP-ribosylation factor-like protein 8B
NM_018184.3	NP_060654.1	ADP-ribosylation factor-like protein 8B

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	ARL8B	XP_414440.3
Danio rerio (zebrafish)	arl8bb	XP_002663861.1
Homo sapiens (human)	ARL8B	NP_060654.1
Canis lupus familiaris (dog)	ARL8B	XP_853013.1
Mus musculus (house mouse)	Arl8b	NP_080287.1
Rattus norvegicus (Norway rat)	Arl8b	NP_001019503.1
	LOC703201	XP_002808365.1
Pan troglodytes (chimpanzee)	ARL8B	XP_001142369.1
Bos taurus (cattle)	ARL8B	NP_001039536.1
Arabidopsis thaliana (thale cress)	ARLA1A	NP_568553.1
Oryza sativa (rice)	Os02g0732500	NP_001048027.1
Xenopus tropicalis (tropical clawed frog)	arl8b	NP_001190182.1

Gene Ontology
Bibliography

Related articles in PubMed

LAMTOR/Ragulator is a negative regulator of Arl8b- and BORC-dependent late endosomal positioning.
Filipek PA, de Araujo MEG, Vogel GF, De Smet CH, Eberharter D, Rebsamen M, Rudashevskaya EL, Kremser L, Yordanov T, Tschaikner P, F?rnrohr BG, Lechner S, Dunzendorfer-Matt T, Scheffzek K, Bennett KL, Superti-Furga G, Lindner HH, Stasyk T, Huber LA
The Journal of cell biology216(12)4199-4215(2017 Dec)

RNF167 targets Arl8B for degradation to regulate lysosome positioning and endocytic trafficking.
Deshar R, Moon S, Yoo W, Cho EB, Yoon SK, Yoon JB
The FEBS journal283(24)4583-4599(2016 12)

The Arf-like GTPase Arl8b is essential for three-dimensional invasive growth of prostate cancer in vitro and xenograft formation and growth in vivo.
Dykes SS, Gray AL, Coleman DT, Saxena M, Stephens CA, Carroll JL, Pruitt K, Cardelli JA
Oncotarget7(21)31037-52(2016 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

LAMTOR1 is a negative regulator of Arl8b- and BORC-dependent late endosomal positioning.
Title: LAMTOR/Ragulator is a negative regulator of Arl8b- and BORC-dependent late endosomal positioning.

Data indicate that E3 ubiquitin-protein ligase RNF167 ubiquitinates Arl8B at the lysine residue K141 and reduces the level of the ADP-ribosylation factor-like 8B Arl8B protein.
Title: RNF167 targets Arl8B for degradation to regulate lysosome positioning and endocytic trafficking.

Authors present evidence that Arl8b facilitates lipid hydrolysis to maintain efficient metabolism for a proliferative capacity in low nutrient environments, suggesting a likely explanation for the complete inability of Arl8b-depleted tumor cells to grow in vivo.
Title: The Arf-like GTPase Arl8b is essential for three-dimensional invasive growth of prostate cancer in vitro and xenograft formation and growth in vivo.

Arl8b regulates the association of the human HOPS complex with lysosomal membranes.
Title: The small GTPase Arl8b regulates assembly of the mammalian HOPS complex on lysosomes.

a small GTP-binding protein, ADP-ribosylation factor-like 8b (Arl8b), is identified as a critical factor required for NK cell-mediated cytotoxicity.
Title: Arf-like GTPase Arl8b regulates lytic granule polarization and natural killer cell-mediated cytotoxicity.

The following ARL8B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARL8B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02123	NM_018184.3 Latest version!	Homo sapiens ADP ribosylation factor like GTPase 8B (ARL8B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu02123	NM_018184.2	Homo sapiens ADP ribosylation factor like GTPase 8B (ARL8B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ARL8B ( NM_018184.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_060654.1 Homo sapiens ADP ribosylation factor like GTPase 8B (ARL8B), mRNA.

Price & Availability↑

CloneID	OHu02123
Clone ID Related Accession (Same CDS sequence)	NM_018184.2 , NM_018184.3
Accession Version	NM_018184.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-10
Organism	Homo sapiens(human)
Product	ADP-ribosylation factor-like protein 8B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC063125.1 and AK001564.1. On Jun 30, 2004 this sequence version replaced NM_018184.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.176720.1, BC063125.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGCTGGCGC TCATCTCCCG CCTGCTGGAC TGGTTCCGTT CGCTCTTCTG GAAGGAAGAG 
ATGGAGCTGA CGCTCGTGGG GCTGCAGTAC TCGGGCAAGA CCACCTTCGT CAATGTCATC 
GCGTCAGGTC AATTCAGTGA AGATATGATA CCCACAGTGG GCTTCAACAT GAGGAAGGTA 
ACTAAAGGTA ACGTCACAAT AAAGATCTGG GACATAGGAG GACAACCCCG ATTTCGAAGC 
ATGTGGGAGC GGTATTGCAG AGGAGTCAAT GCTATTGTTT ACATGATAGA TGCTGCAGAT 
CGTGAAAAGA TAGAAGCTTC CCGAAATGAG CTACATAATC TTCTAGATAA ACCACAGTTA 
CAAGGAATTC CAGTGCTAGT GCTTGGAAAC AAGAGAGATC TTCCTAATGC CTTGGATGAG 
AAACAGCTAA TTGAAAAAAT GAATCTGTCT GCTATTCAGG ATAGAGAAAT TTGCTGCTAT 
TCAATTTCTT GCAAAGAAAA GGATAATATA GATATCACAC TTCAGTGGCT TATTCAGCAT 
TCAAAATCTA GAAGAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060654.1
CDS	222..782
Translation	MLALISRLLDWFRSLFWKEEMELTLVGLQYSGKTTFVNVIASGQFSEDMIPTVGFNMRKVTKGNVTIKIWDIGGQPRFRSMWERYCRGVNAIVYMIDAADREKIEASRNELHNLLDKPQLQGIPVLVLGNKRDLPNALDEKQLIEKMNLSAIQDREICCYSISCKEKDNIDITLQWLIQHSKSRRS

Target ORF information:

RefSeq Version	NM_018184.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ADP ribosylation factor like GTPase 8B (ARL8B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018184.2

ORF Insert Sequence:

ATGCTGGCGC TCATCTCCCG CCTGCTGGAC TGGTTCCGTT CGCTCTTCTG GAAGGAAGAG 
ATGGAGCTGA CGCTCGTGGG GCTGCAGTAC TCGGGCAAGA CCACCTTCGT CAATGTCATC 
GCGTCAGGTC AATTCAGTGA AGATATGATA CCCACAGTGG GCTTCAACAT GAGGAAGGTA 
ACTAAAGGTA ACGTCACAAT AAAGATCTGG GACATAGGAG GACAACCCCG ATTTCGAAGC 
ATGTGGGAGC GGTATTGCAG AGGAGTCAAT GCTATTGTTT ACATGATAGA TGCTGCAGAT 
CGTGAAAAGA TAGAAGCTTC CCGAAATGAG CTACATAATC TTCTAGATAA ACCACAGTTA 
CAAGGAATTC CAGTGCTAGT GCTTGGAAAC AAGAGAGATC TTCCTAATGC CTTGGATGAG 
AAACAGCTAA TTGAAAAAAT GAATCTGTCT GCTATTCAGG ATAGAGAAAT TTGCTGCTAT 
TCAATTTCTT GCAAAGAAAA GGATAATATA GATATCACAC TTCAGTGGCT TATTCAGCAT 
TCAAAATCTA GAAGAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARL8B ( NM_018184.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_060654.1 Homo sapiens ADP ribosylation factor like GTPase 8B (ARL8B), mRNA.

Price & Availability↑

CloneID	OHu02123
Clone ID Related Accession (Same CDS sequence)	NM_018184.2 , NM_018184.3
Accession Version	NM_018184.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	ADP-ribosylation factor-like protein 8B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC063125.1 and AK001564.1. On Nov 23, 2018 this sequence version replaced NM_018184.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.66611.1, SRR1660803.16300.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000256496.8/ ENSP00000256496.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTGGCGC TCATCTCCCG CCTGCTGGAC TGGTTCCGTT CGCTCTTCTG GAAGGAAGAG 
ATGGAGCTGA CGCTCGTGGG GCTGCAGTAC TCGGGCAAGA CCACCTTCGT CAATGTCATC 
GCGTCAGGTC AATTCAGTGA AGATATGATA CCCACAGTGG GCTTCAACAT GAGGAAGGTA 
ACTAAAGGTA ACGTCACAAT AAAGATCTGG GACATAGGAG GACAACCCCG ATTTCGAAGC 
ATGTGGGAGC GGTATTGCAG AGGAGTCAAT GCTATTGTTT ACATGATAGA TGCTGCAGAT 
CGTGAAAAGA TAGAAGCTTC CCGAAATGAG CTACATAATC TTCTAGATAA ACCACAGTTA 
CAAGGAATTC CAGTGCTAGT GCTTGGAAAC AAGAGAGATC TTCCTAATGC CTTGGATGAG 
AAACAGCTAA TTGAAAAAAT GAATCTGTCT GCTATTCAGG ATAGAGAAAT TTGCTGCTAT 
TCAATTTCTT GCAAAGAAAA GGATAATATA GATATCACAC TTCAGTGGCT TATTCAGCAT 
TCAAAATCTA GAAGAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060654.1
CDS	175..735
Translation	MLALISRLLDWFRSLFWKEEMELTLVGLQYSGKTTFVNVIASGQFSEDMIPTVGFNMRKVTKGNVTIKIWDIGGQPRFRSMWERYCRGVNAIVYMIDAADREKIEASRNELHNLLDKPQLQGIPVLVLGNKRDLPNALDEKQLIEKMNLSAIQDREICCYSISCKEKDNIDITLQWLIQHSKSRRS

Target ORF information:

RefSeq Version	NM_018184.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ADP ribosylation factor like GTPase 8B (ARL8B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018184.3

ORF Insert Sequence:

ATGCTGGCGC TCATCTCCCG CCTGCTGGAC TGGTTCCGTT CGCTCTTCTG GAAGGAAGAG 
ATGGAGCTGA CGCTCGTGGG GCTGCAGTAC TCGGGCAAGA CCACCTTCGT CAATGTCATC 
GCGTCAGGTC AATTCAGTGA AGATATGATA CCCACAGTGG GCTTCAACAT GAGGAAGGTA 
ACTAAAGGTA ACGTCACAAT AAAGATCTGG GACATAGGAG GACAACCCCG ATTTCGAAGC 
ATGTGGGAGC GGTATTGCAG AGGAGTCAAT GCTATTGTTT ACATGATAGA TGCTGCAGAT 
CGTGAAAAGA TAGAAGCTTC CCGAAATGAG CTACATAATC TTCTAGATAA ACCACAGTTA 
CAAGGAATTC CAGTGCTAGT GCTTGGAAAC AAGAGAGATC TTCCTAATGC CTTGGATGAG 
AAACAGCTAA TTGAAAAAAT GAATCTGTCT GCTATTCAGG ATAGAGAAAT TTGCTGCTAT 
TCAATTTCTT GCAAAGAAAA GGATAATATA GATATCACAC TTCAGTGGCT TATTCAGCAT 
TCAAAATCTA GAAGAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

LAMTOR/Ragulator is a negative regulator of Arl8b- and BORC-dependent late endosomal positioning.
The Journal of cell biology216(12)4199-4215(2017 Dec)
Filipek PA,de Araujo MEG,Vogel GF,De Smet CH,Eberharter D,Rebsamen M,Rudashevskaya EL,Kremser L,Yordanov T,Tschaikner P,F?rnrohr BG,Lechner S,Dunzendorfer-Matt T,Scheffzek K,Bennett KL,Superti-Furga G,Lindner HH,Stasyk T,Huber LA

RNF167 targets Arl8B for degradation to regulate lysosome positioning and endocytic trafficking.
The FEBS journal283(24)4583-4599(2016 12)
Deshar R,Moon S,Yoo W,Cho EB,Yoon SK,Yoon JB

The Arf-like GTPase Arl8b is essential for three-dimensional invasive growth of prostate cancer in vitro and xenograft formation and growth in vivo.
Oncotarget7(21)31037-52(2016 May)
Dykes SS,Gray AL,Coleman DT,Saxena M,Stephens CA,Carroll JL,Pruitt K,Cardelli JA