NSUN2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NSUN2
Entrez Gene ID	54888
Full Name	NOP2/Sun RNA methyltransferase family member 2
Synonyms	MISU,MRT5,SAKI,TRM4
General protein information	Preferred Names NOP2/Sun RNA methyltransferase family member 2 Names tRNA (cytosine(34)-C(5))-methyltransferase 5-methycytoisine methyltransferase Myc-induced SUN-domain-containing protein NOL1/NOP2/Sun domain family, member 2 NOP2/Sun domain family, member 2 myc-induced SUN domain-containing protein substrate of AIM1/Aurora kinase B tRNA (cytosine-5-)-methyltransferase NSUN2 tRNA methyltransferase 4 homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5p15.31
Summary	This gene encodes a methyltransferase that catalyzes the methylation of cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. This modification is necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Mar 2011].
Disorder MIM:	610916
Disorder Html:	Mental retardation, autosomal recessive 5, 611091 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_017755.5	NP_060225.4	tRNA (cytosine(34)-C(5))-methyltransferase isoform 1
NM_001193455.1	NP_001180384.1	tRNA (cytosine(34)-C(5))-methyltransferase isoform 2
NM_017755.6	NP_060225.4	RNA cytosine C(5)-methyltransferase NSUN2 isoform 1
NM_001193455.2	NP_001180384.1	RNA cytosine C(5)-methyltransferase NSUN2 isoform 2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	NSUN2	NP_001026175.1
Oryza sativa (rice)	Os08g0484400	NP_001062081.1
Canis lupus familiaris (dog)	NSUN2	XP_535800.2
Schizosaccharomyces pombe (fission yeast)	SPAC17D4.04	NP_594695.3
Neurospora crassa	NCU05301	XP_962072.1
Arabidopsis thaliana (thale cress)	AT4G40000	NP_195710.3
Oryza sativa (rice)	Os09g0471900	NP_001063445.1
Pan troglodytes (chimpanzee)	NSUN2	XP_517620.2
Mus musculus (house mouse)	Nsun2	NP_663329.3
Drosophila melanogaster (fruit fly)	Nsun2	NP_652007.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002504	XP_563662.3
Caenorhabditis elegans (roundworm)	Y48G8AL.5	NP_490830.2
Eremothecium gossypii	AGOS_AFR179C	NP_985726.2
Homo sapiens (human)	NSUN2	NP_060225.4
Saccharomyces cerevisiae (baker's yeast)	NCL1	NP_009529.1
Kluyveromyces lactis	KLLA0F12562g	XP_455648.1
	NSUN2	XP_002804365.1
Bos taurus (cattle)	NSUN2	XP_002696495.1
Rattus norvegicus (Norway rat)	Nsun2	NP_001101873.1
Danio rerio (zebrafish)	nsun2	NP_956005.1
Schizosaccharomyces pombe (fission yeast)	SPAC23C4.17	NP_593189.2
Magnaporthe oryzae (rice blast fungus)	MGG_13217	XP_003711942.1
Arabidopsis thaliana (thale cress)	AT2G22400	NP_850024.1
Xenopus tropicalis (tropical clawed frog)	nsun2	NP_001015962.1

Gene Ontology

The following NSUN2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NSUN2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu27121	NM_017755.6 Latest version!	Homo sapiens NOP2/Sun RNA methyltransferase 2 (NSUN2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu27034	NM_001193455.2 Latest version!	Homo sapiens NOP2/Sun RNA methyltransferase 2 (NSUN2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$517.30 $739.00
View Old Accession Versions >>
OHu27121	NM_017755.5	Homo sapiens NOP2/Sun RNA methyltransferase family member 2 (NSUN2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu27034	NM_001193455.1	Homo sapiens NOP2/Sun RNA methyltransferase 2 (NSUN2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$517.30 $739.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NSUN2 ( NM_017755.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_060225.4 Homo sapiens NOP2/Sun RNA methyltransferase family member 2 (NSUN2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27121
Clone ID Related Accession (Same CDS sequence)	NM_017755.5 , NM_017755.6
Accession Version	NM_017755.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-31
Organism	Homo sapiens(human)
Product	tRNA (cytosine(34)-C(5))-methyltransferase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB255451.1, BF000644.1 and BC001041.2. This sequence is a reference standard in the RefSeqGene project. On Jul 27, 2010 this sequence version replaced NM_017755.4. Transcript Variant: This variant (1) represents the predominant transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.8300.1, SRR1660803.168543.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCGGC GGTCGCGGGG TCGGCGGCTC CAGCAACAGC AGCGGCCGGA GGACGCGGAG 
GATGGCGCCG AGGGTGGTGG AAAGCGCGGC GAGGCGGGCT GGGAAGGAGG CTACCCCGAG 
ATCGTCAAGG AGAACAAGCT GTTCGAGCAC TACTACCAGG AGCTCAAGAT CGTGCCCGAG 
GGCGAGTGGG GCCAGTTCAT GGACGCTCTC AGGGAGCCGC TCCCGGCCAC TTTAAGAATT 
ACTGGTTACA AAAGCCACGC AAAAGAGATT CTCCATTGCT TAAAGAACAA ATATTTTAAG 
GAATTGGAGG ACCTGGAGGT GGACGGTCAG AAAGTTGAAG TTCCACAGCC ACTGAGTTGG 
TATCCTGAAG AACTTGCCTG GCACACAAAT TTAAGTCGAA AAATCTTGAG AAAATCGCCA 
CACTTGGAAA AGTTTCATCA GTTTCTAGTT AGTGAAACAG AATCTGGAAA TATTAGTCGT 
CAAGAAGCTG TTAGCATGAT CCCACCACTG CTCCTCAACG TGCGGCCTCA TCATAAGATC 
TTAGATATGT GTGCAGCACC TGGCTCAAAG ACCACACAGT TAATTGAAAT GCTACATGCC 
GACATGAATG TCCCCTTTCC AGAGGGATTT GTTATTGCGA ATGATGTGGA CAACAAGCGC 
TGCTACCTGC TCGTCCATCA AGCCAAGAGG CTGAGCAGCC CCTGCATCAT GGTGGTCAAC 
CATGATGCCT CCAGCATACC CAGGCTCCAG ATAGATGTGG ACGGCAGGAA AGAGATCCTC 
TTCTATGATC GAATTTTATG TGATGTCCCT TGCAGTGGAG ACGGCACTAT GAGAAAAAAC 
ATTGATGTTT GGAAAAAGTG GACCACCTTA AATAGCTTGC AGCTACATGG CTTACAGCTG 
CGGATTGCAA CACGCGGGGC TGAACAGCTG GCTGAAGGTG GAAGGATGGT GTATTCCACG 
TGTTCACTAA ACCCTATTGA GGATGAAGCA GTCATAGCAT CTTTACTGGA AAAAAGTGAA 
GGTGCTTTGG AGCTTGCTGA TGTGTCTAAT GAACTGCCAG GGCTGAAGTG GATGCCTGGA 
ATCACACAGT GGAAGGTAAT GACGAAAGAT GGGCAGTGGT TTACAGACTG GGACGCTGTT 
CCTCACAGCA GACACACCCA GATCCGACCT ACCATGTTCC CTCCGAAGGA CCCAGAAAAG 
CTGCAGGCCA TGCACCTGGA GCGATGCCTT AGGATATTAC CCCATCATCA GAATACTGGA 
GGGTTTTTTG TGGCAGTATT GGTGAAAAAA TCTTCAATGC CGTGGAATAA ACGTCAGCCA 
AAGCTTCAGG GTAAATCTGC AGAGACCAGA GAAAGCACAC AGCTGAGCCC TGCAGATCTC 
ACAGAAGGGA AACCCACAGA TCCCTCTAAG CTGGAAAGTC CGTCATTCAC AGGAACTGGT 
GACACAGAAA TAGCTCATGC AACTGAGGAT TTAGAGAATA ATGGCAGTAA GAAAGATGGC 
GTGTGTGGTC CTCCTCCATC AAAGAAAATG AAGTTATTTG GATTTAAAGA AGATCCATTT 
GTATTTATTC CTGAAGATGA CCCATTATTT CCACCTATTG AGAAATTTTA TGCTTTGGAT 
CCTTCATTCC CAAGGATGAA TTTGTTAACT CGGACTACAG AAGGGAAGAA AAGGCAGCTC 
TACATGGTTT CTAAGGAGTT GCGGAATGTG CTGCTGAATA ACAGTGAGAA GATGAAGGTT 
ATTAACACGG GGATCAAAGT CTGGTGTAGA AATAACAGCG GTGAAGAGTT TGACTGTGCT 
TTCCGGCTGG CACAGGAGGG AATATATACA TTGTATCCAT TTATTAACTC AAGAATTATT 
ACTGTATCAA TGGAAGATGT TAAGATACTG TTGACCCAGG AAAATCCCTT TTTTAGAAAA 
CTCAGCAGTG AGACCTACAG TCAAGCAAAG GACCTGGCAA AGGGAAGCAT CGTGCTGAAG 
TATGAACCAG ATTCTGCGAA TCCAGACGCT CTGCAGTGTC CCATCGTCTT ATGCGGATGG 
CGGGGAAAGG CCTCCATTCG AACTTTTGTG CCCAAGAATG AACGGCTTCA TTATCTCAGG 
ATGATGGGGC TGGAGGTATT GGGAGAAAAG AAGAAGGAAG GGGTTATCCT CACAAATGAG 
AGTGCAGCCA GCACCGGACA GCCAGACAAT GACGTGACTG AGGGACAGAG AGCAGGAGAG 
CCCAACAGCC CAGATGCAGA AGAGGCCAAC AGTCCAGACG TGACAGCAGG CTGTGACCCG 
GCGGGGGTCC ATCCACCCCG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060225.4
CDS	382..2685
Translation	MGRRSRGRRLQQQQRPEDAEDGAEGGGKRGEAGWEGGYPEIVKENKLFEHYYQELKIVPEGEWGQFMDALREPLPATLRITGYKSHAKEILHCLKNKYFKELEDLEVDGQKVEVPQPLSWYPEELAWHTNLSRKILRKSPHLEKFHQFLVSETESGNISRQEAVSMIPPLLLNVRPHHKILDMCAAPGSKTTQLIEMLHADMNVPFPEGFVIANDVDNKRCYLLVHQAKRLSSPCIMVVNHDASSIPRLQIDVDGRKEILFYDRILCDVPCSGDGTMRKNIDVWKKWTTLNSLQLHGLQLRIATRGAEQLAEGGRMVYSTCSLNPIEDEAVIASLLEKSEGALELADVSNELPGLKWMPGITQWKVMTKDGQWFTDWDAVPHSRHTQIRPTMFPPKDPEKLQAMHLERCLRILPHHQNTGGFFVAVLVKKSSMPWNKRQPKLQGKSAETRESTQLSPADLTEGKPTDPSKLESPSFTGTGDTEIAHATEDLENNGSKKDGVCGPPPSKKMKLFGFKEDPFVFIPEDDPLFPPIEKFYALDPSFPRMNLLTRTTEGKKRQLYMVSKELRNVLLNNSEKMKVINTGIKVWCRNNSGEEFDCAFRLAQEGIYTLYPFINSRIITVSMEDVKILLTQENPFFRKLSSETYSQAKDLAKGSIVLKYEPDSANPDALQCPIVLCGWRGKASIRTFVPKNERLHYLRMMGLEVLGEKKKEGVILTNESAASTGQPDNDVTEGQRAGEPNSPDAEEANSPDVTAGCDPAGVHPPR

Target ORF information:

RefSeq Version	NM_017755.5
Organism	Homo sapiens(human)
Definition	Homo sapiens NOP2/Sun RNA methyltransferase family member 2 (NSUN2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017755.5

ORF Insert Sequence:

ATGGGGCGGC GGTCGCGGGG TCGGCGGCTC CAGCAACAGC AGCGGCCGGA GGACGCGGAG 
GATGGCGCCG AGGGTGGTGG AAAGCGCGGC GAGGCGGGCT GGGAAGGAGG CTACCCCGAG 
ATCGTCAAGG AGAACAAGCT GTTCGAGCAC TACTACCAGG AGCTCAAGAT CGTGCCCGAG 
GGCGAGTGGG GCCAGTTCAT GGACGCTCTC AGGGAGCCGC TCCCGGCCAC TTTAAGAATT 
ACTGGTTACA AAAGCCACGC AAAAGAGATT CTCCATTGCT TAAAGAACAA ATATTTTAAG 
GAATTGGAGG ACCTGGAGGT GGACGGTCAG AAAGTTGAAG TTCCACAGCC ACTGAGTTGG 
TATCCTGAAG AACTTGCCTG GCACACAAAT TTAAGTCGAA AAATCTTGAG AAAATCGCCA 
CACTTGGAAA AGTTTCATCA GTTTCTAGTT AGTGAAACAG AATCTGGAAA TATTAGTCGT 
CAAGAAGCTG TTAGCATGAT CCCACCACTG CTCCTCAACG TGCGGCCTCA TCATAAGATC 
TTAGATATGT GTGCAGCACC TGGCTCAAAG ACCACACAGT TAATTGAAAT GCTACATGCC 
GACATGAATG TCCCCTTTCC AGAGGGATTT GTTATTGCGA ATGATGTGGA CAACAAGCGC 
TGCTACCTGC TCGTCCATCA AGCCAAGAGG CTGAGCAGCC CCTGCATCAT GGTGGTCAAC 
CATGATGCCT CCAGCATACC CAGGCTCCAG ATAGATGTGG ACGGCAGGAA AGAGATCCTC 
TTCTATGATC GAATTTTATG TGATGTCCCT TGCAGTGGAG ACGGCACTAT GAGAAAAAAC 
ATTGATGTTT GGAAAAAGTG GACCACCTTA AATAGCTTGC AGCTACATGG CTTACAGCTG 
CGGATTGCAA CACGCGGGGC TGAACAGCTG GCTGAAGGTG GAAGGATGGT GTATTCCACG 
TGTTCACTAA ACCCTATTGA GGATGAAGCA GTCATAGCAT CTTTACTGGA AAAAAGTGAA 
GGTGCTTTGG AGCTTGCTGA TGTGTCTAAT GAACTGCCAG GGCTGAAGTG GATGCCTGGA 
ATCACACAGT GGAAGGTAAT GACGAAAGAT GGGCAGTGGT TTACAGACTG GGACGCTGTT 
CCTCACAGCA GACACACCCA GATCCGACCT ACCATGTTCC CTCCGAAGGA CCCAGAAAAG 
CTGCAGGCCA TGCACCTGGA GCGATGCCTT AGGATATTAC CCCATCATCA GAATACTGGA 
GGGTTTTTTG TGGCAGTATT GGTGAAAAAA TCTTCAATGC CGTGGAATAA ACGTCAGCCA 
AAGCTTCAGG GTAAATCTGC AGAGACCAGA GAAAGCACAC AGCTGAGCCC TGCAGATCTC 
ACAGAAGGGA AACCCACAGA TCCCTCTAAG CTGGAAAGTC CGTCATTCAC AGGAACTGGT 
GACACAGAAA TAGCTCATGC AACTGAGGAT TTAGAGAATA ATGGCAGTAA GAAAGATGGC 
GTGTGTGGTC CTCCTCCATC AAAGAAAATG AAGTTATTTG GATTTAAAGA AGATCCATTT 
GTATTTATTC CTGAAGATGA CCCATTATTT CCACCTATTG AGAAATTTTA TGCTTTGGAT 
CCTTCATTCC CAAGGATGAA TTTGTTAACT CGGACTACAG AAGGGAAGAA AAGGCAGCTC 
TACATGGTTT CTAAGGAGTT GCGGAATGTG CTGCTGAATA ACAGTGAGAA GATGAAGGTT 
ATTAACACGG GGATCAAAGT CTGGTGTAGA AATAACAGCG GTGAAGAGTT TGACTGTGCT 
TTCCGGCTGG CACAGGAGGG AATATATACA TTGTATCCAT TTATTAACTC AAGAATTATT 
ACTGTATCAA TGGAAGATGT TAAGATACTG TTGACCCAGG AAAATCCCTT TTTTAGAAAA 
CTCAGCAGTG AGACCTACAG TCAAGCAAAG GACCTGGCAA AGGGAAGCAT CGTGCTGAAG 
TATGAACCAG ATTCTGCGAA TCCAGACGCT CTGCAGTGTC CCATCGTCTT ATGCGGATGG 
CGGGGAAAGG CCTCCATTCG AACTTTTGTG CCCAAGAATG AACGGCTTCA TTATCTCAGG 
ATGATGGGGC TGGAGGTATT GGGAGAAAAG AAGAAGGAAG GGGTTATCCT CACAAATGAG 
AGTGCAGCCA GCACCGGACA GCCAGACAAT GACGTGACTG AGGGACAGAG AGCAGGAGAG 
CCCAACAGCC CAGATGCAGA AGAGGCCAAC AGTCCAGACG TGACAGCAGG CTGTGACCCG 
GCGGGGGTCC ATCCACCCCG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NSUN2 ( NM_017755.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_060225.4 Homo sapiens NOP2/Sun RNA methyltransferase 2 (NSUN2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27121
Clone ID Related Accession (Same CDS sequence)	NM_017755.5 , NM_017755.6
Accession Version	NM_017755.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	RNA cytosine C(5)-methyltransferase NSUN2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF000644.1, AB255451.1 and BC001041.2. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_017755.5. Transcript Variant: This variant (1) represents the predominant transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.249220.1, SRR1660807.8300.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000264670.11/ ENSP00000264670.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGGCGGC GGTCGCGGGG TCGGCGGCTC CAGCAACAGC AGCGGCCGGA GGACGCGGAG 
GATGGCGCCG AGGGTGGTGG AAAGCGCGGC GAGGCGGGCT GGGAAGGAGG CTACCCCGAG 
ATCGTCAAGG AGAACAAGCT GTTCGAGCAC TACTACCAGG AGCTCAAGAT CGTGCCCGAG 
GGCGAGTGGG GCCAGTTCAT GGACGCTCTC AGGGAGCCGC TCCCGGCCAC TTTAAGAATT 
ACTGGTTACA AAAGCCACGC AAAAGAGATT CTCCATTGCT TAAAGAACAA ATATTTTAAG 
GAATTGGAGG ACCTGGAGGT GGACGGTCAG AAAGTTGAAG TTCCACAGCC ACTGAGTTGG 
TATCCTGAAG AACTTGCCTG GCACACAAAT TTAAGTCGAA AAATCTTGAG AAAATCGCCA 
CACTTGGAAA AGTTTCATCA GTTTCTAGTT AGTGAAACAG AATCTGGAAA TATTAGTCGT 
CAAGAAGCTG TTAGCATGAT CCCACCACTG CTCCTCAACG TGCGGCCTCA TCATAAGATC 
TTAGATATGT GTGCAGCACC TGGCTCAAAG ACCACACAGT TAATTGAAAT GCTACATGCC 
GACATGAATG TCCCCTTTCC AGAGGGATTT GTTATTGCGA ATGATGTGGA CAACAAGCGC 
TGCTACCTGC TCGTCCATCA AGCCAAGAGG CTGAGCAGCC CCTGCATCAT GGTGGTCAAC 
CATGATGCCT CCAGCATACC CAGGCTCCAG ATAGATGTGG ACGGCAGGAA AGAGATCCTC 
TTCTATGATC GAATTTTATG TGATGTCCCT TGCAGTGGAG ACGGCACTAT GAGAAAAAAC 
ATTGATGTTT GGAAAAAGTG GACCACCTTA AATAGCTTGC AGCTACATGG CTTACAGCTG 
CGGATTGCAA CACGCGGGGC TGAACAGCTG GCTGAAGGTG GAAGGATGGT GTATTCCACG 
TGTTCACTAA ACCCTATTGA GGATGAAGCA GTCATAGCAT CTTTACTGGA AAAAAGTGAA 
GGTGCTTTGG AGCTTGCTGA TGTGTCTAAT GAACTGCCAG GGCTGAAGTG GATGCCTGGA 
ATCACACAGT GGAAGGTAAT GACGAAAGAT GGGCAGTGGT TTACAGACTG GGACGCTGTT 
CCTCACAGCA GACACACCCA GATCCGACCT ACCATGTTCC CTCCGAAGGA CCCAGAAAAG 
CTGCAGGCCA TGCACCTGGA GCGATGCCTT AGGATATTAC CCCATCATCA GAATACTGGA 
GGGTTTTTTG TGGCAGTATT GGTGAAAAAA TCTTCAATGC CGTGGAATAA ACGTCAGCCA 
AAGCTTCAGG GTAAATCTGC AGAGACCAGA GAAAGCACAC AGCTGAGCCC TGCAGATCTC 
ACAGAAGGGA AACCCACAGA TCCCTCTAAG CTGGAAAGTC CGTCATTCAC AGGAACTGGT 
GACACAGAAA TAGCTCATGC AACTGAGGAT TTAGAGAATA ATGGCAGTAA GAAAGATGGC 
GTGTGTGGTC CTCCTCCATC AAAGAAAATG AAGTTATTTG GATTTAAAGA AGATCCATTT 
GTATTTATTC CTGAAGATGA CCCATTATTT CCACCTATTG AGAAATTTTA TGCTTTGGAT 
CCTTCATTCC CAAGGATGAA TTTGTTAACT CGGACTACAG AAGGGAAGAA AAGGCAGCTC 
TACATGGTTT CTAAGGAGTT GCGGAATGTG CTGCTGAATA ACAGTGAGAA GATGAAGGTT 
ATTAACACGG GGATCAAAGT CTGGTGTAGA AATAACAGCG GTGAAGAGTT TGACTGTGCT 
TTCCGGCTGG CACAGGAGGG AATATATACA TTGTATCCAT TTATTAACTC AAGAATTATT 
ACTGTATCAA TGGAAGATGT TAAGATACTG TTGACCCAGG AAAATCCCTT TTTTAGAAAA 
CTCAGCAGTG AGACCTACAG TCAAGCAAAG GACCTGGCAA AGGGAAGCAT CGTGCTGAAG 
TATGAACCAG ATTCTGCGAA TCCAGACGCT CTGCAGTGTC CCATCGTCTT ATGCGGATGG 
CGGGGAAAGG CCTCCATTCG AACTTTTGTG CCCAAGAATG AACGGCTTCA TTATCTCAGG 
ATGATGGGGC TGGAGGTATT GGGAGAAAAG AAGAAGGAAG GGGTTATCCT CACAAATGAG 
AGTGCAGCCA GCACCGGACA GCCAGACAAT GACGTGACTG AGGGACAGAG AGCAGGAGAG 
CCCAACAGCC CAGATGCAGA AGAGGCCAAC AGTCCAGACG TGACAGCAGG CTGTGACCCG 
GCGGGGGTCC ATCCACCCCG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060225.4
CDS	66..2369
Translation	MGRRSRGRRLQQQQRPEDAEDGAEGGGKRGEAGWEGGYPEIVKENKLFEHYYQELKIVPEGEWGQFMDALREPLPATLRITGYKSHAKEILHCLKNKYFKELEDLEVDGQKVEVPQPLSWYPEELAWHTNLSRKILRKSPHLEKFHQFLVSETESGNISRQEAVSMIPPLLLNVRPHHKILDMCAAPGSKTTQLIEMLHADMNVPFPEGFVIANDVDNKRCYLLVHQAKRLSSPCIMVVNHDASSIPRLQIDVDGRKEILFYDRILCDVPCSGDGTMRKNIDVWKKWTTLNSLQLHGLQLRIATRGAEQLAEGGRMVYSTCSLNPIEDEAVIASLLEKSEGALELADVSNELPGLKWMPGITQWKVMTKDGQWFTDWDAVPHSRHTQIRPTMFPPKDPEKLQAMHLERCLRILPHHQNTGGFFVAVLVKKSSMPWNKRQPKLQGKSAETRESTQLSPADLTEGKPTDPSKLESPSFTGTGDTEIAHATEDLENNGSKKDGVCGPPPSKKMKLFGFKEDPFVFIPEDDPLFPPIEKFYALDPSFPRMNLLTRTTEGKKRQLYMVSKELRNVLLNNSEKMKVINTGIKVWCRNNSGEEFDCAFRLAQEGIYTLYPFINSRIITVSMEDVKILLTQENPFFRKLSSETYSQAKDLAKGSIVLKYEPDSANPDALQCPIVLCGWRGKASIRTFVPKNERLHYLRMMGLEVLGEKKKEGVILTNESAASTGQPDNDVTEGQRAGEPNSPDAEEANSPDVTAGCDPAGVHPPR

Target ORF information:

RefSeq Version	NM_017755.6
Organism	Homo sapiens(human)
Definition	Homo sapiens NOP2/Sun RNA methyltransferase 2 (NSUN2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017755.6

ORF Insert Sequence:

ATGGGGCGGC GGTCGCGGGG TCGGCGGCTC CAGCAACAGC AGCGGCCGGA GGACGCGGAG 
GATGGCGCCG AGGGTGGTGG AAAGCGCGGC GAGGCGGGCT GGGAAGGAGG CTACCCCGAG 
ATCGTCAAGG AGAACAAGCT GTTCGAGCAC TACTACCAGG AGCTCAAGAT CGTGCCCGAG 
GGCGAGTGGG GCCAGTTCAT GGACGCTCTC AGGGAGCCGC TCCCGGCCAC TTTAAGAATT 
ACTGGTTACA AAAGCCACGC AAAAGAGATT CTCCATTGCT TAAAGAACAA ATATTTTAAG 
GAATTGGAGG ACCTGGAGGT GGACGGTCAG AAAGTTGAAG TTCCACAGCC ACTGAGTTGG 
TATCCTGAAG AACTTGCCTG GCACACAAAT TTAAGTCGAA AAATCTTGAG AAAATCGCCA 
CACTTGGAAA AGTTTCATCA GTTTCTAGTT AGTGAAACAG AATCTGGAAA TATTAGTCGT 
CAAGAAGCTG TTAGCATGAT CCCACCACTG CTCCTCAACG TGCGGCCTCA TCATAAGATC 
TTAGATATGT GTGCAGCACC TGGCTCAAAG ACCACACAGT TAATTGAAAT GCTACATGCC 
GACATGAATG TCCCCTTTCC AGAGGGATTT GTTATTGCGA ATGATGTGGA CAACAAGCGC 
TGCTACCTGC TCGTCCATCA AGCCAAGAGG CTGAGCAGCC CCTGCATCAT GGTGGTCAAC 
CATGATGCCT CCAGCATACC CAGGCTCCAG ATAGATGTGG ACGGCAGGAA AGAGATCCTC 
TTCTATGATC GAATTTTATG TGATGTCCCT TGCAGTGGAG ACGGCACTAT GAGAAAAAAC 
ATTGATGTTT GGAAAAAGTG GACCACCTTA AATAGCTTGC AGCTACATGG CTTACAGCTG 
CGGATTGCAA CACGCGGGGC TGAACAGCTG GCTGAAGGTG GAAGGATGGT GTATTCCACG 
TGTTCACTAA ACCCTATTGA GGATGAAGCA GTCATAGCAT CTTTACTGGA AAAAAGTGAA 
GGTGCTTTGG AGCTTGCTGA TGTGTCTAAT GAACTGCCAG GGCTGAAGTG GATGCCTGGA 
ATCACACAGT GGAAGGTAAT GACGAAAGAT GGGCAGTGGT TTACAGACTG GGACGCTGTT 
CCTCACAGCA GACACACCCA GATCCGACCT ACCATGTTCC CTCCGAAGGA CCCAGAAAAG 
CTGCAGGCCA TGCACCTGGA GCGATGCCTT AGGATATTAC CCCATCATCA GAATACTGGA 
GGGTTTTTTG TGGCAGTATT GGTGAAAAAA TCTTCAATGC CGTGGAATAA ACGTCAGCCA 
AAGCTTCAGG GTAAATCTGC AGAGACCAGA GAAAGCACAC AGCTGAGCCC TGCAGATCTC 
ACAGAAGGGA AACCCACAGA TCCCTCTAAG CTGGAAAGTC CGTCATTCAC AGGAACTGGT 
GACACAGAAA TAGCTCATGC AACTGAGGAT TTAGAGAATA ATGGCAGTAA GAAAGATGGC 
GTGTGTGGTC CTCCTCCATC AAAGAAAATG AAGTTATTTG GATTTAAAGA AGATCCATTT 
GTATTTATTC CTGAAGATGA CCCATTATTT CCACCTATTG AGAAATTTTA TGCTTTGGAT 
CCTTCATTCC CAAGGATGAA TTTGTTAACT CGGACTACAG AAGGGAAGAA AAGGCAGCTC 
TACATGGTTT CTAAGGAGTT GCGGAATGTG CTGCTGAATA ACAGTGAGAA GATGAAGGTT 
ATTAACACGG GGATCAAAGT CTGGTGTAGA AATAACAGCG GTGAAGAGTT TGACTGTGCT 
TTCCGGCTGG CACAGGAGGG AATATATACA TTGTATCCAT TTATTAACTC AAGAATTATT 
ACTGTATCAA TGGAAGATGT TAAGATACTG TTGACCCAGG AAAATCCCTT TTTTAGAAAA 
CTCAGCAGTG AGACCTACAG TCAAGCAAAG GACCTGGCAA AGGGAAGCAT CGTGCTGAAG 
TATGAACCAG ATTCTGCGAA TCCAGACGCT CTGCAGTGTC CCATCGTCTT ATGCGGATGG 
CGGGGAAAGG CCTCCATTCG AACTTTTGTG CCCAAGAATG AACGGCTTCA TTATCTCAGG 
ATGATGGGGC TGGAGGTATT GGGAGAAAAG AAGAAGGAAG GGGTTATCCT CACAAATGAG 
AGTGCAGCCA GCACCGGACA GCCAGACAAT GACGTGACTG AGGGACAGAG AGCAGGAGAG 
CCCAACAGCC CAGATGCAGA AGAGGCCAAC AGTCCAGACG TGACAGCAGG CTGTGACCCG 
GCGGGGGTCC ATCCACCCCG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NSUN2 ( NM_001193455.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001180384.1 Homo sapiens NOP2/Sun RNA methyltransferase 2 (NSUN2), transcript variant 2, mRNA.

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CloneID	OHu27034
Clone ID Related Accession (Same CDS sequence)	NM_001193455.1 , NM_001193455.2
Accession Version	NM_001193455.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	tRNA (cytosine(34)-C(5))-methyltransferase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB255451.1, BF000644.1, BG387061.1, AK298980.1 and BC001041.2. Transcript Variant: This variant (2) lacks an in-frame coding exon in the 5' region compared to variant 1. This results in a shorter isoform (2) missing an internal protein segment compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.142452.1, SRR1803616.33587.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCGGC GGTCGCGGGG TCGGCGGCTC CAGCAACAGC AGCGGCCGGA GGACGCGGAG 
GATGGCGCCG AGGGTGGTGG AAAGCGCGGC GAGGCGGGCT GGGAAGGAGG CTACCCCGAG 
ATCGTCAAGG AGAACAAGCT GTTCGAGCAC TACTACCAGG AGCTCAAGAT CGTGCCCGAG 
GGCGAGTGGG GCCAGTTCAT GGACGCTCTC AGGGAGCCGC TCCCGGCCAC TTTAAGAATT 
ACTGGTTACA AAAGGTATCC TGAAGAACTT GCCTGGCACA CAAATTTAAG TCGAAAAATC 
TTGAGAAAAT CGCCACACTT GGAAAAGTTT CATCAGTTTC TAGTTAGTGA AACAGAATCT 
GGAAATATTA GTCGTCAAGA AGCTGTTAGC ATGATCCCAC CACTGCTCCT CAACGTGCGG 
CCTCATCATA AGATCTTAGA TATGTGTGCA GCACCTGGCT CAAAGACCAC ACAGTTAATT 
GAAATGCTAC ATGCCGACAT GAATGTCCCC TTTCCAGAGG GATTTGTTAT TGCGAATGAT 
GTGGACAACA AGCGCTGCTA CCTGCTCGTC CATCAAGCCA AGAGGCTGAG CAGCCCCTGC 
ATCATGGTGG TCAACCATGA TGCCTCCAGC ATACCCAGGC TCCAGATAGA TGTGGACGGC 
AGGAAAGAGA TCCTCTTCTA TGATCGAATT TTATGTGATG TCCCTTGCAG TGGAGACGGC 
ACTATGAGAA AAAACATTGA TGTTTGGAAA AAGTGGACCA CCTTAAATAG CTTGCAGCTA 
CATGGCTTAC AGCTGCGGAT TGCAACACGC GGGGCTGAAC AGCTGGCTGA AGGTGGAAGG 
ATGGTGTATT CCACGTGTTC ACTAAACCCT ATTGAGGATG AAGCAGTCAT AGCATCTTTA 
CTGGAAAAAA GTGAAGGTGC TTTGGAGCTT GCTGATGTGT CTAATGAACT GCCAGGGCTG 
AAGTGGATGC CTGGAATCAC ACAGTGGAAG GTAATGACGA AAGATGGGCA GTGGTTTACA 
GACTGGGACG CTGTTCCTCA CAGCAGACAC ACCCAGATCC GACCTACCAT GTTCCCTCCG 
AAGGACCCAG AAAAGCTGCA GGCCATGCAC CTGGAGCGAT GCCTTAGGAT ATTACCCCAT 
CATCAGAATA CTGGAGGGTT TTTTGTGGCA GTATTGGTGA AAAAATCTTC AATGCCGTGG 
AATAAACGTC AGCCAAAGCT TCAGGGTAAA TCTGCAGAGA CCAGAGAAAG CACACAGCTG 
AGCCCTGCAG ATCTCACAGA AGGGAAACCC ACAGATCCCT CTAAGCTGGA AAGTCCGTCA 
TTCACAGGAA CTGGTGACAC AGAAATAGCT CATGCAACTG AGGATTTAGA GAATAATGGC 
AGTAAGAAAG ATGGCGTGTG TGGTCCTCCT CCATCAAAGA AAATGAAGTT ATTTGGATTT 
AAAGAAGATC CATTTGTATT TATTCCTGAA GATGACCCAT TATTTCCACC TATTGAGAAA 
TTTTATGCTT TGGATCCTTC ATTCCCAAGG ATGAATTTGT TAACTCGGAC TACAGAAGGG 
AAGAAAAGGC AGCTCTACAT GGTTTCTAAG GAGTTGCGGA ATGTGCTGCT GAATAACAGT 
GAGAAGATGA AGGTTATTAA CACGGGGATC AAAGTCTGGT GTAGAAATAA CAGCGGTGAA 
GAGTTTGACT GTGCTTTCCG GCTGGCACAG GAGGGAATAT ATACATTGTA TCCATTTATT 
AACTCAAGAA TTATTACTGT ATCAATGGAA GATGTTAAGA TACTGTTGAC CCAGGAAAAT 
CCCTTTTTTA GAAAACTCAG CAGTGAGACC TACAGTCAAG CAAAGGACCT GGCAAAGGGA 
AGCATCGTGC TGAAGTATGA ACCAGATTCT GCGAATCCAG ACGCTCTGCA GTGTCCCATC 
GTCTTATGCG GATGGCGGGG AAAGGCCTCC ATTCGAACTT TTGTGCCCAA GAATGAACGG 
CTTCATTATC TCAGGATGAT GGGGCTGGAG GTATTGGGAG AAAAGAAGAA GGAAGGGGTT 
ATCCTCACAA ATGAGAGTGC AGCCAGCACC GGACAGCCAG ACAATGACGT GACTGAGGGA 
CAGAGAGCAG GAGAGCCCAA CAGCCCAGAT GCAGAAGAGG CCAACAGTCC AGACGTGACA 
GCAGGCTGTG ACCCGGCGGG GGTCCATCCA CCCCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001180384.1
CDS	382..2580
Translation	MGRRSRGRRLQQQQRPEDAEDGAEGGGKRGEAGWEGGYPEIVKENKLFEHYYQELKIVPEGEWGQFMDALREPLPATLRITGYKRYPEELAWHTNLSRKILRKSPHLEKFHQFLVSETESGNISRQEAVSMIPPLLLNVRPHHKILDMCAAPGSKTTQLIEMLHADMNVPFPEGFVIANDVDNKRCYLLVHQAKRLSSPCIMVVNHDASSIPRLQIDVDGRKEILFYDRILCDVPCSGDGTMRKNIDVWKKWTTLNSLQLHGLQLRIATRGAEQLAEGGRMVYSTCSLNPIEDEAVIASLLEKSEGALELADVSNELPGLKWMPGITQWKVMTKDGQWFTDWDAVPHSRHTQIRPTMFPPKDPEKLQAMHLERCLRILPHHQNTGGFFVAVLVKKSSMPWNKRQPKLQGKSAETRESTQLSPADLTEGKPTDPSKLESPSFTGTGDTEIAHATEDLENNGSKKDGVCGPPPSKKMKLFGFKEDPFVFIPEDDPLFPPIEKFYALDPSFPRMNLLTRTTEGKKRQLYMVSKELRNVLLNNSEKMKVINTGIKVWCRNNSGEEFDCAFRLAQEGIYTLYPFINSRIITVSMEDVKILLTQENPFFRKLSSETYSQAKDLAKGSIVLKYEPDSANPDALQCPIVLCGWRGKASIRTFVPKNERLHYLRMMGLEVLGEKKKEGVILTNESAASTGQPDNDVTEGQRAGEPNSPDAEEANSPDVTAGCDPAGVHPPR

Target ORF information:

RefSeq Version	NM_001193455.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NOP2/Sun RNA methyltransferase 2 (NSUN2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193455.1

ORF Insert Sequence:

ATGGGGCGGC GGTCGCGGGG TCGGCGGCTC CAGCAACAGC AGCGGCCGGA GGACGCGGAG 
GATGGCGCCG AGGGTGGTGG AAAGCGCGGC GAGGCGGGCT GGGAAGGAGG CTACCCCGAG 
ATCGTCAAGG AGAACAAGCT GTTCGAGCAC TACTACCAGG AGCTCAAGAT CGTGCCCGAG 
GGCGAGTGGG GCCAGTTCAT GGACGCTCTC AGGGAGCCGC TCCCGGCCAC TTTAAGAATT 
ACTGGTTACA AAAGGTATCC TGAAGAACTT GCCTGGCACA CAAATTTAAG TCGAAAAATC 
TTGAGAAAAT CGCCACACTT GGAAAAGTTT CATCAGTTTC TAGTTAGTGA AACAGAATCT 
GGAAATATTA GTCGTCAAGA AGCTGTTAGC ATGATCCCAC CACTGCTCCT CAACGTGCGG 
CCTCATCATA AGATCTTAGA TATGTGTGCA GCACCTGGCT CAAAGACCAC ACAGTTAATT 
GAAATGCTAC ATGCCGACAT GAATGTCCCC TTTCCAGAGG GATTTGTTAT TGCGAATGAT 
GTGGACAACA AGCGCTGCTA CCTGCTCGTC CATCAAGCCA AGAGGCTGAG CAGCCCCTGC 
ATCATGGTGG TCAACCATGA TGCCTCCAGC ATACCCAGGC TCCAGATAGA TGTGGACGGC 
AGGAAAGAGA TCCTCTTCTA TGATCGAATT TTATGTGATG TCCCTTGCAG TGGAGACGGC 
ACTATGAGAA AAAACATTGA TGTTTGGAAA AAGTGGACCA CCTTAAATAG CTTGCAGCTA 
CATGGCTTAC AGCTGCGGAT TGCAACACGC GGGGCTGAAC AGCTGGCTGA AGGTGGAAGG 
ATGGTGTATT CCACGTGTTC ACTAAACCCT ATTGAGGATG AAGCAGTCAT AGCATCTTTA 
CTGGAAAAAA GTGAAGGTGC TTTGGAGCTT GCTGATGTGT CTAATGAACT GCCAGGGCTG 
AAGTGGATGC CTGGAATCAC ACAGTGGAAG GTAATGACGA AAGATGGGCA GTGGTTTACA 
GACTGGGACG CTGTTCCTCA CAGCAGACAC ACCCAGATCC GACCTACCAT GTTCCCTCCG 
AAGGACCCAG AAAAGCTGCA GGCCATGCAC CTGGAGCGAT GCCTTAGGAT ATTACCCCAT 
CATCAGAATA CTGGAGGGTT TTTTGTGGCA GTATTGGTGA AAAAATCTTC AATGCCGTGG 
AATAAACGTC AGCCAAAGCT TCAGGGTAAA TCTGCAGAGA CCAGAGAAAG CACACAGCTG 
AGCCCTGCAG ATCTCACAGA AGGGAAACCC ACAGATCCCT CTAAGCTGGA AAGTCCGTCA 
TTCACAGGAA CTGGTGACAC AGAAATAGCT CATGCAACTG AGGATTTAGA GAATAATGGC 
AGTAAGAAAG ATGGCGTGTG TGGTCCTCCT CCATCAAAGA AAATGAAGTT ATTTGGATTT 
AAAGAAGATC CATTTGTATT TATTCCTGAA GATGACCCAT TATTTCCACC TATTGAGAAA 
TTTTATGCTT TGGATCCTTC ATTCCCAAGG ATGAATTTGT TAACTCGGAC TACAGAAGGG 
AAGAAAAGGC AGCTCTACAT GGTTTCTAAG GAGTTGCGGA ATGTGCTGCT GAATAACAGT 
GAGAAGATGA AGGTTATTAA CACGGGGATC AAAGTCTGGT GTAGAAATAA CAGCGGTGAA 
GAGTTTGACT GTGCTTTCCG GCTGGCACAG GAGGGAATAT ATACATTGTA TCCATTTATT 
AACTCAAGAA TTATTACTGT ATCAATGGAA GATGTTAAGA TACTGTTGAC CCAGGAAAAT 
CCCTTTTTTA GAAAACTCAG CAGTGAGACC TACAGTCAAG CAAAGGACCT GGCAAAGGGA 
AGCATCGTGC TGAAGTATGA ACCAGATTCT GCGAATCCAG ACGCTCTGCA GTGTCCCATC 
GTCTTATGCG GATGGCGGGG AAAGGCCTCC ATTCGAACTT TTGTGCCCAA GAATGAACGG 
CTTCATTATC TCAGGATGAT GGGGCTGGAG GTATTGGGAG AAAAGAAGAA GGAAGGGGTT 
ATCCTCACAA ATGAGAGTGC AGCCAGCACC GGACAGCCAG ACAATGACGT GACTGAGGGA 
CAGAGAGCAG GAGAGCCCAA CAGCCCAGAT GCAGAAGAGG CCAACAGTCC AGACGTGACA 
GCAGGCTGTG ACCCGGCGGG GGTCCATCCA CCCCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NSUN2 ( NM_001193455.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001180384.1 Homo sapiens NOP2/Sun RNA methyltransferase 2 (NSUN2), transcript variant 2, mRNA.

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CloneID	OHu27034
Clone ID Related Accession (Same CDS sequence)	NM_001193455.1 , NM_001193455.2
Accession Version	NM_001193455.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Homo sapiens(human)
Product	RNA cytosine C(5)-methyltransferase NSUN2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF000644.1, BG387061.1, AK298980.1 and BC001041.2. On May 31, 2019 this sequence version replaced NM_001193455.1. Transcript Variant: This variant (2) lacks an in-frame coding exon in the 5' region compared to variant 1. This results in a shorter isoform (2) missing an internal protein segment compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.142452.1, SRR1803616.33587.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGCGGC GGTCGCGGGG TCGGCGGCTC CAGCAACAGC AGCGGCCGGA GGACGCGGAG 
GATGGCGCCG AGGGTGGTGG AAAGCGCGGC GAGGCGGGCT GGGAAGGAGG CTACCCCGAG 
ATCGTCAAGG AGAACAAGCT GTTCGAGCAC TACTACCAGG AGCTCAAGAT CGTGCCCGAG 
GGCGAGTGGG GCCAGTTCAT GGACGCTCTC AGGGAGCCGC TCCCGGCCAC TTTAAGAATT 
ACTGGTTACA AAAGGTATCC TGAAGAACTT GCCTGGCACA CAAATTTAAG TCGAAAAATC 
TTGAGAAAAT CGCCACACTT GGAAAAGTTT CATCAGTTTC TAGTTAGTGA AACAGAATCT 
GGAAATATTA GTCGTCAAGA AGCTGTTAGC ATGATCCCAC CACTGCTCCT CAACGTGCGG 
CCTCATCATA AGATCTTAGA TATGTGTGCA GCACCTGGCT CAAAGACCAC ACAGTTAATT 
GAAATGCTAC ATGCCGACAT GAATGTCCCC TTTCCAGAGG GATTTGTTAT TGCGAATGAT 
GTGGACAACA AGCGCTGCTA CCTGCTCGTC CATCAAGCCA AGAGGCTGAG CAGCCCCTGC 
ATCATGGTGG TCAACCATGA TGCCTCCAGC ATACCCAGGC TCCAGATAGA TGTGGACGGC 
AGGAAAGAGA TCCTCTTCTA TGATCGAATT TTATGTGATG TCCCTTGCAG TGGAGACGGC 
ACTATGAGAA AAAACATTGA TGTTTGGAAA AAGTGGACCA CCTTAAATAG CTTGCAGCTA 
CATGGCTTAC AGCTGCGGAT TGCAACACGC GGGGCTGAAC AGCTGGCTGA AGGTGGAAGG 
ATGGTGTATT CCACGTGTTC ACTAAACCCT ATTGAGGATG AAGCAGTCAT AGCATCTTTA 
CTGGAAAAAA GTGAAGGTGC TTTGGAGCTT GCTGATGTGT CTAATGAACT GCCAGGGCTG 
AAGTGGATGC CTGGAATCAC ACAGTGGAAG GTAATGACGA AAGATGGGCA GTGGTTTACA 
GACTGGGACG CTGTTCCTCA CAGCAGACAC ACCCAGATCC GACCTACCAT GTTCCCTCCG 
AAGGACCCAG AAAAGCTGCA GGCCATGCAC CTGGAGCGAT GCCTTAGGAT ATTACCCCAT 
CATCAGAATA CTGGAGGGTT TTTTGTGGCA GTATTGGTGA AAAAATCTTC AATGCCGTGG 
AATAAACGTC AGCCAAAGCT TCAGGGTAAA TCTGCAGAGA CCAGAGAAAG CACACAGCTG 
AGCCCTGCAG ATCTCACAGA AGGGAAACCC ACAGATCCCT CTAAGCTGGA AAGTCCGTCA 
TTCACAGGAA CTGGTGACAC AGAAATAGCT CATGCAACTG AGGATTTAGA GAATAATGGC 
AGTAAGAAAG ATGGCGTGTG TGGTCCTCCT CCATCAAAGA AAATGAAGTT ATTTGGATTT 
AAAGAAGATC CATTTGTATT TATTCCTGAA GATGACCCAT TATTTCCACC TATTGAGAAA 
TTTTATGCTT TGGATCCTTC ATTCCCAAGG ATGAATTTGT TAACTCGGAC TACAGAAGGG 
AAGAAAAGGC AGCTCTACAT GGTTTCTAAG GAGTTGCGGA ATGTGCTGCT GAATAACAGT 
GAGAAGATGA AGGTTATTAA CACGGGGATC AAAGTCTGGT GTAGAAATAA CAGCGGTGAA 
GAGTTTGACT GTGCTTTCCG GCTGGCACAG GAGGGAATAT ATACATTGTA TCCATTTATT 
AACTCAAGAA TTATTACTGT ATCAATGGAA GATGTTAAGA TACTGTTGAC CCAGGAAAAT 
CCCTTTTTTA GAAAACTCAG CAGTGAGACC TACAGTCAAG CAAAGGACCT GGCAAAGGGA 
AGCATCGTGC TGAAGTATGA ACCAGATTCT GCGAATCCAG ACGCTCTGCA GTGTCCCATC 
GTCTTATGCG GATGGCGGGG AAAGGCCTCC ATTCGAACTT TTGTGCCCAA GAATGAACGG 
CTTCATTATC TCAGGATGAT GGGGCTGGAG GTATTGGGAG AAAAGAAGAA GGAAGGGGTT 
ATCCTCACAA ATGAGAGTGC AGCCAGCACC GGACAGCCAG ACAATGACGT GACTGAGGGA 
CAGAGAGCAG GAGAGCCCAA CAGCCCAGAT GCAGAAGAGG CCAACAGTCC AGACGTGACA 
GCAGGCTGTG ACCCGGCGGG GGTCCATCCA CCCCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001180384.1
CDS	66..2264
Translation	MGRRSRGRRLQQQQRPEDAEDGAEGGGKRGEAGWEGGYPEIVKENKLFEHYYQELKIVPEGEWGQFMDALREPLPATLRITGYKRYPEELAWHTNLSRKILRKSPHLEKFHQFLVSETESGNISRQEAVSMIPPLLLNVRPHHKILDMCAAPGSKTTQLIEMLHADMNVPFPEGFVIANDVDNKRCYLLVHQAKRLSSPCIMVVNHDASSIPRLQIDVDGRKEILFYDRILCDVPCSGDGTMRKNIDVWKKWTTLNSLQLHGLQLRIATRGAEQLAEGGRMVYSTCSLNPIEDEAVIASLLEKSEGALELADVSNELPGLKWMPGITQWKVMTKDGQWFTDWDAVPHSRHTQIRPTMFPPKDPEKLQAMHLERCLRILPHHQNTGGFFVAVLVKKSSMPWNKRQPKLQGKSAETRESTQLSPADLTEGKPTDPSKLESPSFTGTGDTEIAHATEDLENNGSKKDGVCGPPPSKKMKLFGFKEDPFVFIPEDDPLFPPIEKFYALDPSFPRMNLLTRTTEGKKRQLYMVSKELRNVLLNNSEKMKVINTGIKVWCRNNSGEEFDCAFRLAQEGIYTLYPFINSRIITVSMEDVKILLTQENPFFRKLSSETYSQAKDLAKGSIVLKYEPDSANPDALQCPIVLCGWRGKASIRTFVPKNERLHYLRMMGLEVLGEKKKEGVILTNESAASTGQPDNDVTEGQRAGEPNSPDAEEANSPDVTAGCDPAGVHPPR

Target ORF information:

RefSeq Version	NM_001193455.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NOP2/Sun RNA methyltransferase 2 (NSUN2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193455.2

ORF Insert Sequence:

ATGGGGCGGC GGTCGCGGGG TCGGCGGCTC CAGCAACAGC AGCGGCCGGA GGACGCGGAG 
GATGGCGCCG AGGGTGGTGG AAAGCGCGGC GAGGCGGGCT GGGAAGGAGG CTACCCCGAG 
ATCGTCAAGG AGAACAAGCT GTTCGAGCAC TACTACCAGG AGCTCAAGAT CGTGCCCGAG 
GGCGAGTGGG GCCAGTTCAT GGACGCTCTC AGGGAGCCGC TCCCGGCCAC TTTAAGAATT 
ACTGGTTACA AAAGGTATCC TGAAGAACTT GCCTGGCACA CAAATTTAAG TCGAAAAATC 
TTGAGAAAAT CGCCACACTT GGAAAAGTTT CATCAGTTTC TAGTTAGTGA AACAGAATCT 
GGAAATATTA GTCGTCAAGA AGCTGTTAGC ATGATCCCAC CACTGCTCCT CAACGTGCGG 
CCTCATCATA AGATCTTAGA TATGTGTGCA GCACCTGGCT CAAAGACCAC ACAGTTAATT 
GAAATGCTAC ATGCCGACAT GAATGTCCCC TTTCCAGAGG GATTTGTTAT TGCGAATGAT 
GTGGACAACA AGCGCTGCTA CCTGCTCGTC CATCAAGCCA AGAGGCTGAG CAGCCCCTGC 
ATCATGGTGG TCAACCATGA TGCCTCCAGC ATACCCAGGC TCCAGATAGA TGTGGACGGC 
AGGAAAGAGA TCCTCTTCTA TGATCGAATT TTATGTGATG TCCCTTGCAG TGGAGACGGC 
ACTATGAGAA AAAACATTGA TGTTTGGAAA AAGTGGACCA CCTTAAATAG CTTGCAGCTA 
CATGGCTTAC AGCTGCGGAT TGCAACACGC GGGGCTGAAC AGCTGGCTGA AGGTGGAAGG 
ATGGTGTATT CCACGTGTTC ACTAAACCCT ATTGAGGATG AAGCAGTCAT AGCATCTTTA 
CTGGAAAAAA GTGAAGGTGC TTTGGAGCTT GCTGATGTGT CTAATGAACT GCCAGGGCTG 
AAGTGGATGC CTGGAATCAC ACAGTGGAAG GTAATGACGA AAGATGGGCA GTGGTTTACA 
GACTGGGACG CTGTTCCTCA CAGCAGACAC ACCCAGATCC GACCTACCAT GTTCCCTCCG 
AAGGACCCAG AAAAGCTGCA GGCCATGCAC CTGGAGCGAT GCCTTAGGAT ATTACCCCAT 
CATCAGAATA CTGGAGGGTT TTTTGTGGCA GTATTGGTGA AAAAATCTTC AATGCCGTGG 
AATAAACGTC AGCCAAAGCT TCAGGGTAAA TCTGCAGAGA CCAGAGAAAG CACACAGCTG 
AGCCCTGCAG ATCTCACAGA AGGGAAACCC ACAGATCCCT CTAAGCTGGA AAGTCCGTCA 
TTCACAGGAA CTGGTGACAC AGAAATAGCT CATGCAACTG AGGATTTAGA GAATAATGGC 
AGTAAGAAAG ATGGCGTGTG TGGTCCTCCT CCATCAAAGA AAATGAAGTT ATTTGGATTT 
AAAGAAGATC CATTTGTATT TATTCCTGAA GATGACCCAT TATTTCCACC TATTGAGAAA 
TTTTATGCTT TGGATCCTTC ATTCCCAAGG ATGAATTTGT TAACTCGGAC TACAGAAGGG 
AAGAAAAGGC AGCTCTACAT GGTTTCTAAG GAGTTGCGGA ATGTGCTGCT GAATAACAGT 
GAGAAGATGA AGGTTATTAA CACGGGGATC AAAGTCTGGT GTAGAAATAA CAGCGGTGAA 
GAGTTTGACT GTGCTTTCCG GCTGGCACAG GAGGGAATAT ATACATTGTA TCCATTTATT 
AACTCAAGAA TTATTACTGT ATCAATGGAA GATGTTAAGA TACTGTTGAC CCAGGAAAAT 
CCCTTTTTTA GAAAACTCAG CAGTGAGACC TACAGTCAAG CAAAGGACCT GGCAAAGGGA 
AGCATCGTGC TGAAGTATGA ACCAGATTCT GCGAATCCAG ACGCTCTGCA GTGTCCCATC 
GTCTTATGCG GATGGCGGGG AAAGGCCTCC ATTCGAACTT TTGTGCCCAA GAATGAACGG 
CTTCATTATC TCAGGATGAT GGGGCTGGAG GTATTGGGAG AAAAGAAGAA GGAAGGGGTT 
ATCCTCACAA ATGAGAGTGC AGCCAGCACC GGACAGCCAG ACAATGACGT GACTGAGGGA 
CAGAGAGCAG GAGAGCCCAA CAGCCCAGAT GCAGAAGAGG CCAACAGTCC AGACGTGACA 
GCAGGCTGTG ACCCGGCGGG GGTCCATCCA CCCCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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NSUN2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム