PPIB cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PPIB
Entrez Gene ID	5479
Full Name	peptidylprolyl isomerase B
Synonyms	CYP-S1,CYPB,HEL-S-39,OI9,SCYLP
General protein information	Preferred Names peptidylprolyl isomerase B Names peptidyl-prolyl cis-trans isomerase B PPIase B S-cyclophilin cyclophilin-like protein epididymis secretory protein Li 39 peptidylprolyl isomerase B (cyclophilin B) rotamase B
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q22.31
Summary	The protein encoded by this gene is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Variants have been identified in this protein that give rise to recessive forms of osteogenesis imperfecta. [provided by RefSeq, Oct 2009].
Disorder MIM:	123841
Disorder Html:	Osteogenesis imperfecta, type IX, 259440 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000942.4	NP_000933.1	peptidyl-prolyl cis-trans isomerase B precursor
NM_000942.5	NP_000933.1	peptidyl-prolyl cis-trans isomerase B precursor

Pathways from BioSystems

Homologies

	PPIB	XP_001106590.1
Canis lupus familiaris (dog)	PPIB	XP_852389.1
Rattus norvegicus (Norway rat)	Ppib	NP_071981.1
Danio rerio (zebrafish)	ppib	NP_998184.1
Saccharomyces cerevisiae (baker's yeast)	CPR5	NP_010590.3
Kluyveromyces lactis	KLLA0C10208g	XP_452654.1
Bos taurus (cattle)	PPIB	NP_776577.1
Drosophila melanogaster (fruit fly)	CG2852	NP_611695.1
Schizosaccharomyces pombe (fission yeast)	cyp4	NP_596532.1
Neurospora crassa	NCU01200	XP_001728305.1
Homo sapiens (human)	PPIB	NP_000933.1
Pan troglodytes (chimpanzee)	PPIB	XP_003952716.1
Mus musculus (house mouse)	Ppib	NP_035279.2
Gallus gallus (chicken)	PPIB	NP_990792.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007088	XP_308669.2
Eremothecium gossypii	AGOS_AAL056C	NP_982486.1
Arabidopsis thaliana (thale cress)	CYP20-2	NP_001190299.1
Caenorhabditis elegans (roundworm)	cyn-6	NP_497257.1
Caenorhabditis elegans (roundworm)	cyn-5	NP_493624.1
Magnaporthe oryzae (rice blast fungus)	MGG_15814	XP_003715036.1
Oryza sativa (rice)	Os05g0103200	NP_001054392.1
Xenopus tropicalis (tropical clawed frog)	ppib	NP_001017063.1

Gene Ontology
Bibliography

Related articles in PubMed

Human Cyclophilin B forms part of a multi-protein complex during erythrocyte invasion by Plasmodium falciparum.
Prakash P, Zeeshan M, Saini E, Muneer A, Khurana S, Kumar Chourasia B, Deshmukh A, Kaur I, Dabral S, Singh N, Anam Z, Chaurasiya A, Kaushik S, Dahiya P, Kalamuddin M, Kumar Thakur J, Mohmmed A, Ranganathan A, Malhotra P
Nature communications8(1)1548(2017 Nov)

Ziploc-ing the structure 2.0: Endoplasmic reticulum-resident peptidyl prolyl isomerases show different activities toward hydroxyproline.
Ishikawa Y, Mizuno K, B?chinger HP
The Journal of biological chemistry292(22)9273-9282(2017 06)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

observed changes in activity of six rER-resident PPIases, cyclophilin B (encoded by the PPIB gene), FKBP13 (FKBP2), FKBP19 (FKBP11), FKBP22 (FKBP14), FKBP23 (FKBP7), and FKBP65 (FKBP10), due to posttranslational modifications of proline residues in the substrate.
Title: Ziploc-ing the structure 2.0: Endoplasmic reticulum-resident peptidyl prolyl isomerases show different activities toward hydroxyproline.

PPIB mutations and their associated phenotypes
Title: Two novel mutations in the PPIB gene cause a rare pedigree of osteogenesis imperfecta type IX.

The data suggest that overexpressed CypB protects neuronal cells from MPP+-induced dopaminergic neuronal cell death
Title: Cyclophilin B protects SH-SY5Y human neuroblastoma cells against MPP(+)-induced neurotoxicity via JNK pathway.

Over-expression of CypB enhances HIV infection by increasing nuclear import of viral DNA.
Title: Cyclophilin B enhances HIV-1 infection.

This study enhances our knowledge about the mutational pattern of the LEPRE1, CRTAP, and PPIB genes. LEPRE1 should be the first gene analyzed in mutation detection studies in patients with recessive OI.
Title: Mutational characterization of the P3H1/CRTAP/CypB complex in recessive osteogenesis imperfecta.

The following PPIB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPIB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19649	NM_000942.5 Latest version!	Homo sapiens peptidylprolyl isomerase B (PPIB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
View Old Accession Versions >>
OHu19649	NM_000942.4	Homo sapiens peptidylprolyl isomerase B (PPIB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPIB ( NM_000942.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000933.1 Homo sapiens peptidylprolyl isomerase B (PPIB), mRNA.

Price & Availability↑

CloneID	OHu19649
Clone ID Related Accession (Same CDS sequence)	NM_000942.4 , NM_000942.5
Accession Version	NM_000942.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Homo sapiens(human)
Product	peptidyl-prolyl cis-trans isomerase B precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BQ931949.1, BC008848.2 and BC001125.2. This sequence is a reference standard in the RefSeqGene project. On Mar 2, 2004 this sequence version replaced NM_000942.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU794651.1, SRR1163658.412242.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGCGCC TCTCCGAACG CAACATGAAG GTGCTCCTTG CCGCCGCCCT CATCGCGGGG 
TCCGTCTTCT TCCTGCTGCT GCCGGGACCT TCTGCGGCCG ATGAGAAGAA GAAGGGGCCC 
AAAGTCACCG TCAAGGTGTA TTTTGACCTA CGAATTGGAG ATGAAGATGT AGGCCGGGTG 
ATCTTTGGTC TCTTCGGAAA GACTGTTCCA AAAACAGTGG ATAATTTTGT GGCCTTAGCT 
ACAGGAGAGA AAGGATTTGG CTACAAAAAC AGCAAATTCC ATCGTGTAAT CAAGGACTTC 
ATGATCCAGG GCGGAGACTT CACCAGGGGA GATGGCACAG GAGGAAAGAG CATCTACGGT 
GAGCGCTTCC CCGATGAGAA CTTCAAACTG AAGCACTACG GGCCTGGCTG GGTGAGCATG 
GCCAACGCAG GCAAAGACAC CAACGGCTCC CAGTTCTTCA TCACGACAGT CAAGACAGCC 
TGGCTAGATG GCAAGCATGT GGTGTTTGGC AAAGTTCTAG AGGGCATGGA GGTGGTGCGG 
AAGGTGGAGA GCACCAAGAC AGACAGCCGG GATAAACCCC TGAAGGATGT GATCATCGCA 
GACTGCGGCA AGATCGAGGT GGAGAAGCCC TTTGCCATCG CCAAGGAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000933.1
CDS	170..820
Translation	MLRLSERNMKVLLAAALIAGSVFFLLLPGPSAADEKKKGPKVTVKVYFDLRIGDEDVGRVIFGLFGKTVPKTVDNFVALATGEKGFGYKNSKFHRVIKDFMIQGGDFTRGDGTGGKSIYGERFPDENFKLKHYGPGWVSMANAGKDTNGSQFFITTVKTAWLDGKHVVFGKVLEGMEVVRKVESTKTDSRDKPLKDVIIADCGKIEVEKPFAIAKE

Target ORF information:

RefSeq Version	NM_000942.4
Organism	Homo sapiens(human)
Definition	Homo sapiens peptidylprolyl isomerase B (PPIB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000942.4

ORF Insert Sequence:

ATGCTGCGCC TCTCCGAACG CAACATGAAG GTGCTCCTTG CCGCCGCCCT CATCGCGGGG 
TCCGTCTTCT TCCTGCTGCT GCCGGGACCT TCTGCGGCCG ATGAGAAGAA GAAGGGGCCC 
AAAGTCACCG TCAAGGTGTA TTTTGACCTA CGAATTGGAG ATGAAGATGT AGGCCGGGTG 
ATCTTTGGTC TCTTCGGAAA GACTGTTCCA AAAACAGTGG ATAATTTTGT GGCCTTAGCT 
ACAGGAGAGA AAGGATTTGG CTACAAAAAC AGCAAATTCC ATCGTGTAAT CAAGGACTTC 
ATGATCCAGG GCGGAGACTT CACCAGGGGA GATGGCACAG GAGGAAAGAG CATCTACGGT 
GAGCGCTTCC CCGATGAGAA CTTCAAACTG AAGCACTACG GGCCTGGCTG GGTGAGCATG 
GCCAACGCAG GCAAAGACAC CAACGGCTCC CAGTTCTTCA TCACGACAGT CAAGACAGCC 
TGGCTAGATG GCAAGCATGT GGTGTTTGGC AAAGTTCTAG AGGGCATGGA GGTGGTGCGG 
AAGGTGGAGA GCACCAAGAC AGACAGCCGG GATAAACCCC TGAAGGATGT GATCATCGCA 
GACTGCGGCA AGATCGAGGT GGAGAAGCCC TTTGCCATCG CCAAGGAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPIB ( NM_000942.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_000933.1 Homo sapiens peptidylprolyl isomerase B (PPIB), mRNA.

Price & Availability↑

CloneID	OHu19649
Clone ID Related Accession (Same CDS sequence)	NM_000942.4 , NM_000942.5
Accession Version	NM_000942.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Homo sapiens(human)
Product	peptidyl-prolyl cis-trans isomerase B precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC008848.2 and BC001125.2. On Dec 4, 2019 this sequence version replaced NM_000942.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX421459.2, BQ276360.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGCGCC TCTCCGAACG CAACATGAAG GTGCTCCTTG CCGCCGCCCT CATCGCGGGG 
TCCGTCTTCT TCCTGCTGCT GCCGGGACCT TCTGCGGCCG ATGAGAAGAA GAAGGGGCCC 
AAAGTCACCG TCAAGGTGTA TTTTGACCTA CGAATTGGAG ATGAAGATGT AGGCCGGGTG 
ATCTTTGGTC TCTTCGGAAA GACTGTTCCA AAAACAGTGG ATAATTTTGT GGCCTTAGCT 
ACAGGAGAGA AAGGATTTGG CTACAAAAAC AGCAAATTCC ATCGTGTAAT CAAGGACTTC 
ATGATCCAGG GCGGAGACTT CACCAGGGGA GATGGCACAG GAGGAAAGAG CATCTACGGT 
GAGCGCTTCC CCGATGAGAA CTTCAAACTG AAGCACTACG GGCCTGGCTG GGTGAGCATG 
GCCAACGCAG GCAAAGACAC CAACGGCTCC CAGTTCTTCA TCACGACAGT CAAGACAGCC 
TGGCTAGATG GCAAGCATGT GGTGTTTGGC AAAGTTCTAG AGGGCATGGA GGTGGTGCGG 
AAGGTGGAGA GCACCAAGAC AGACAGCCGG GATAAACCCC TGAAGGATGT GATCATCGCA 
GACTGCGGCA AGATCGAGGT GGAGAAGCCC TTTGCCATCG CCAAGGAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000933.1
CDS	37..687
Translation	MLRLSERNMKVLLAAALIAGSVFFLLLPGPSAADEKKKGPKVTVKVYFDLRIGDEDVGRVIFGLFGKTVPKTVDNFVALATGEKGFGYKNSKFHRVIKDFMIQGGDFTRGDGTGGKSIYGERFPDENFKLKHYGPGWVSMANAGKDTNGSQFFITTVKTAWLDGKHVVFGKVLEGMEVVRKVESTKTDSRDKPLKDVIIADCGKIEVEKPFAIAKE

Target ORF information:

RefSeq Version	NM_000942.5
Organism	Homo sapiens(human)
Definition	Homo sapiens peptidylprolyl isomerase B (PPIB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000942.5

ORF Insert Sequence:

ATGCTGCGCC TCTCCGAACG CAACATGAAG GTGCTCCTTG CCGCCGCCCT CATCGCGGGG 
TCCGTCTTCT TCCTGCTGCT GCCGGGACCT TCTGCGGCCG ATGAGAAGAA GAAGGGGCCC 
AAAGTCACCG TCAAGGTGTA TTTTGACCTA CGAATTGGAG ATGAAGATGT AGGCCGGGTG 
ATCTTTGGTC TCTTCGGAAA GACTGTTCCA AAAACAGTGG ATAATTTTGT GGCCTTAGCT 
ACAGGAGAGA AAGGATTTGG CTACAAAAAC AGCAAATTCC ATCGTGTAAT CAAGGACTTC 
ATGATCCAGG GCGGAGACTT CACCAGGGGA GATGGCACAG GAGGAAAGAG CATCTACGGT 
GAGCGCTTCC CCGATGAGAA CTTCAAACTG AAGCACTACG GGCCTGGCTG GGTGAGCATG 
GCCAACGCAG GCAAAGACAC CAACGGCTCC CAGTTCTTCA TCACGACAGT CAAGACAGCC 
TGGCTAGATG GCAAGCATGT GGTGTTTGGC AAAGTTCTAG AGGGCATGGA GGTGGTGCGG 
AAGGTGGAGA GCACCAAGAC AGACAGCCGG GATAAACCCC TGAAGGATGT GATCATCGCA 
GACTGCGGCA AGATCGAGGT GGAGAAGCCC TTTGCCATCG CCAAGGAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Human Cyclophilin B forms part of a multi-protein complex during erythrocyte invasion by Plasmodium falciparum.
Nature communications8(1)1548(2017 Nov)
Prakash P,Zeeshan M,Saini E,Muneer A,Khurana S,Kumar Chourasia B,Deshmukh A,Kaur I,Dabral S,Singh N,Anam Z,Chaurasiya A,Kaushik S,Dahiya P,Kalamuddin M,Kumar Thakur J,Mohmmed A,Ranganathan A,Malhotra P

Ziploc-ing the structure 2.0: Endoplasmic reticulum-resident peptidyl prolyl isomerases show different activities toward hydroxyproline.
The Journal of biological chemistry292(22)9273-9282(2017 06)
Ishikawa Y,Mizuno K,B?chinger HP