PPA1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PPA1
Entrez Gene ID	5464
Full Name	pyrophosphatase (inorganic) 1
Synonyms	HEL-S-66p,IOPPP,PP,PP1,SID6-8061
General protein information	Preferred Names pyrophosphatase (inorganic) 1 Names inorganic pyrophosphatase PPase cytosolic inorganic pyrophosphatase diphosphate phosphohydrolase epididymis secretory sperm binding protein Li 66p inorganic diphosphatase 1 inorganic pyrophosphatase 1 pyrophosphatase 1 pyrophosphate phospho-hydrolase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10q22.1
Summary	The protein encoded by this gene is a member of the inorganic pyrophosphatase (PPase) family. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Studies of a similar protein in bovine suggested a cytoplasmic localization of this enzyme. [provided by RefSeq, Jul 2008].
Disorder MIM:	179030

mRNA and Protein(s)

mRNA	Protein	Name
NM_021129.3	NP_066952.1	inorganic pyrophosphatase
NM_021129.4	NP_066952.1	inorganic pyrophosphatase

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Nurf-38	NP_523849.3
Magnaporthe oryzae (rice blast fungus)	MGG_15739	XP_003714580.1
	LOC716720	XP_001107614.2
Bos taurus (cattle)	PPA1	NP_001068586.1
Rattus norvegicus (Norway rat)	Ppa1	NP_001094304.1
Homo sapiens (human)	PPA1	NP_066952.1
Canis lupus familiaris (dog)	PPA1	XP_005619491.1
Saccharomyces cerevisiae (baker's yeast)	IPP1	NP_009565.1
Neurospora crassa	NCU00951	XP_965144.2
Oryza sativa (rice)	Os02g0768600	NP_001048239.1
Pan troglodytes (chimpanzee)	PPA1	XP_001164495.2
Gallus gallus (chicken)	PPA1	XP_001232700.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003398	XP_003436467.1
Caenorhabditis elegans (roundworm)	pyp-1	NP_001023075.1
Schizosaccharomyces pombe (fission yeast)	SPAC23C11.05	NP_593636.1
Arabidopsis thaliana (thale cress)	PPa6	NP_196527.1
Mus musculus (house mouse)	Ppa1	NP_080714.2
Danio rerio (zebrafish)	ppa1b	NP_001017833.1
Kluyveromyces lactis	KLLA0E17667g	XP_454746.1
Eremothecium gossypii	AGOS_AER015C	NP_984875.1
Xenopus tropicalis (tropical clawed frog)	ppa2	NP_001011461.1

Gene Ontology
Bibliography

Related articles in PubMed

p37/UBXN2B regulates spindle orientation by limiting cortical NuMA recruitment via PP1/Repo-Man.
Lee BH, Schwager F, Meraldi P, Gotta M
The Journal of cell biology(2017 Dec)

Expression of Inorganic Pyrophosphatase (PPA1) Correlates with Poor Prognosis of Epithelial Ovarian Cancer.
Li H, Xiao N, Li Z, Wang Q
The Tohoku journal of experimental medicine241(2)165-173(2017 02)

Clinical significance and functional validation of PPA1 in various tumors.
Luo D, Wang G, Shen W, Zhao S, Zhou W, Wan L, Yuan L, Yang S, Xiang R
Cancer medicine5(10)2800-2812(2016 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PPA1 serves as a potential prognostic biomarker for patients with Ovarian serous carcinoma.
Title: Expression of Inorganic Pyrophosphatase (PPA1) Correlates with Poor Prognosis of Epithelial Ovarian Cancer.

Results show that PPA1 expression is significantly higher in many tumors, especially those of lung and ovarian origin, which suggests that PPA1 plays an important role in carcinogenesis and in the development of some tumors.
Title: Clinical significance and functional validation of PPA1 in various tumors.

enhanced NEK2 expression in hepatocellular carcinoma (HCC) promotes HCC progression and drug resistance by promoting PP1/Akt and Wnt pathway activation, which may represent a new therapeutic target for HCC.
Title: Increased NEK2 in hepatocellular carcinoma promotes cancer progression and drug resistance by promoting PP1/Akt and Wnt activation.

Proteomic profiling identifies the inorganic pyrophosphatase (PPA1) protein as a potential biomarker of metastasis in laryngeal squamous cell carcinoma.
Title: Proteomic profiling identifies the inorganic pyrophosphatase (PPA1) protein as a potential biomarker of metastasis in laryngeal squamous cell carcinoma.

Thus, the Ska complex, specifically the Ska1 C-terminal domain, recruits PP1 to kinetochores to oppose spindle checkpoint signaling kinases and promote anaphase onset.
Title: The human SKA complex drives the metaphase-anaphase cell cycle transition by recruiting protein phosphatase 1 to kinetochores.

The following PPA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu01406	NM_021129.4 Latest version!	Homo sapiens inorganic pyrophosphatase 1 (PPA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
View Old Accession Versions >>
OHu01406	NM_021129.3	Homo sapiens pyrophosphatase (inorganic) 1 (PPA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPA1 ( NM_021129.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_066952.1 Homo sapiens pyrophosphatase (inorganic) 1 (PPA1), mRNA.

Price & Availability↑

CloneID	OHu01406
Clone ID Related Accession (Same CDS sequence)	NM_021129.3 , NM_021129.4
Accession Version	NM_021129.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 870bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-10
Organism	Homo sapiens(human)
Product	inorganic pyrophosphatase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL731540.4 and AB026723.1. On Oct 31, 2005 this sequence version replaced NM_021129.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU794626.1, SRR1660803.3985.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCGGCT TCAGCACCGA GGAGCGCGCC GCGCCCTTCT CCCTGGAGTA CCGAGTCTTC 
CTCAAAAATG AGAAAGGACA ATATATATCT CCATTTCATG ATATTCCAAT TTATGCAGAT 
AAGGATGTGT TTCACATGGT AGTTGAAGTA CCACGCTGGT CTAATGCAAA AATGGAGATT 
GCTACAAAGG ACCCTTTAAA CCCTATTAAA CAAGATGTGA AAAAAGGAAA ACTTCGCTAT 
GTTGCGAATT TGTTCCCGTA TAAAGGATAT ATCTGGAACT ATGGTGCCAT CCCTCAGACT 
TGGGAAGACC CAGGGCACAA TGATAAACAT ACTGGCTGTT GTGGTGACAA TGACCCAATT 
GATGTGTGTG AAATTGGAAG CAAGGTATGT GCAAGAGGTG AAATAATTGG CGTGAAAGTT 
CTAGGCATAT TGGCTATGAT TGACGAAGGG GAAACCGACT GGAAAGTCAT TGCCATTAAT 
GTGGATGATC CTGATGCAGC CAATTATAAT GATATCAATG ATGTCAAACG GCTGAAACCT 
GGCTACTTAG AAGCTACTGT GGACTGGTTT AGAAGGTATA AGGTTCCTGA TGGAAAACCA 
GAAAATGAGT TTGCGTTTAA TGCAGAATTT AAAGATAAGG ACTTTGCCAT TGATATTATT 
AAAAGCACTC ATGACCATTG GAAAGCATTA GTGACTAAGA AAACGAATGG AAAAGGAATC 
AGTTGCATGA ATACAACTTT GTCTGAGAGC CCCTTCAAGT GTGATCCTGA TGCTGCCAGA 
GCCATTGTGG ATGCTTTACC ACCACCCTGT GAATCTGCCT GCACAGTACC AACAGACGTG 
GATAAGTGGT TCCATCACCA GAAAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066952.1
CDS	108..977
Translation	MSGFSTEERAAPFSLEYRVFLKNEKGQYISPFHDIPIYADKDVFHMVVEVPRWSNAKMEIATKDPLNPIKQDVKKGKLRYVANLFPYKGYIWNYGAIPQTWEDPGHNDKHTGCCGDNDPIDVCEIGSKVCARGEIIGVKVLGILAMIDEGETDWKVIAINVDDPDAANYNDINDVKRLKPGYLEATVDWFRRYKVPDGKPENEFAFNAEFKDKDFAIDIIKSTHDHWKALVTKKTNGKGISCMNTTLSESPFKCDPDAARAIVDALPPPCESACTVPTDVDKWFHHQKN

Target ORF information:

RefSeq Version	NM_021129.3
Organism	Homo sapiens(human)
Definition	Homo sapiens pyrophosphatase (inorganic) 1 (PPA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021129.3

ORF Insert Sequence:

ATGAGCGGCT TCAGCACCGA GGAGCGCGCC GCGCCCTTCT CCCTGGAGTA CCGAGTCTTC 
CTCAAAAATG AGAAAGGACA ATATATATCT CCATTTCATG ATATTCCAAT TTATGCAGAT 
AAGGATGTGT TTCACATGGT AGTTGAAGTA CCACGCTGGT CTAATGCAAA AATGGAGATT 
GCTACAAAGG ACCCTTTAAA CCCTATTAAA CAAGATGTGA AAAAAGGAAA ACTTCGCTAT 
GTTGCGAATT TGTTCCCGTA TAAAGGATAT ATCTGGAACT ATGGTGCCAT CCCTCAGACT 
TGGGAAGACC CAGGGCACAA TGATAAACAT ACTGGCTGTT GTGGTGACAA TGACCCAATT 
GATGTGTGTG AAATTGGAAG CAAGGTATGT GCAAGAGGTG AAATAATTGG CGTGAAAGTT 
CTAGGCATAT TGGCTATGAT TGACGAAGGG GAAACCGACT GGAAAGTCAT TGCCATTAAT 
GTGGATGATC CTGATGCAGC CAATTATAAT GATATCAATG ATGTCAAACG GCTGAAACCT 
GGCTACTTAG AAGCTACTGT GGACTGGTTT AGAAGGTATA AGGTTCCTGA TGGAAAACCA 
GAAAATGAGT TTGCGTTTAA TGCAGAATTT AAAGATAAGG ACTTTGCCAT TGATATTATT 
AAAAGCACTC ATGACCATTG GAAAGCATTA GTGACTAAGA AAACGAATGG AAAAGGAATC 
AGTTGCATGA ATACAACTTT GTCTGAGAGC CCCTTCAAGT GTGATCCTGA TGCTGCCAGA 
GCCATTGTGG ATGCTTTACC ACCACCCTGT GAATCTGCCT GCACAGTACC AACAGACGTG 
GATAAGTGGT TCCATCACCA GAAAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPA1 ( NM_021129.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_066952.1 Homo sapiens inorganic pyrophosphatase 1 (PPA1), mRNA.

Price & Availability↑

CloneID	OHu01406
Clone ID Related Accession (Same CDS sequence)	NM_021129.3 , NM_021129.4
Accession Version	NM_021129.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 870bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	inorganic pyrophosphatase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL731540.4 and AB026723.1. On Nov 22, 2018 this sequence version replaced NM_021129.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.440882.1, SRR3476690.930360.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000373232.8/ ENSP00000362329.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGCGGCT TCAGCACCGA GGAGCGCGCC GCGCCCTTCT CCCTGGAGTA CCGAGTCTTC 
CTCAAAAATG AGAAAGGACA ATATATATCT CCATTTCATG ATATTCCAAT TTATGCAGAT 
AAGGATGTGT TTCACATGGT AGTTGAAGTA CCACGCTGGT CTAATGCAAA AATGGAGATT 
GCTACAAAGG ACCCTTTAAA CCCTATTAAA CAAGATGTGA AAAAAGGAAA ACTTCGCTAT 
GTTGCGAATT TGTTCCCGTA TAAAGGATAT ATCTGGAACT ATGGTGCCAT CCCTCAGACT 
TGGGAAGACC CAGGGCACAA TGATAAACAT ACTGGCTGTT GTGGTGACAA TGACCCAATT 
GATGTGTGTG AAATTGGAAG CAAGGTATGT GCAAGAGGTG AAATAATTGG CGTGAAAGTT 
CTAGGCATAT TGGCTATGAT TGACGAAGGG GAAACCGACT GGAAAGTCAT TGCCATTAAT 
GTGGATGATC CTGATGCAGC CAATTATAAT GATATCAATG ATGTCAAACG GCTGAAACCT 
GGCTACTTAG AAGCTACTGT GGACTGGTTT AGAAGGTATA AGGTTCCTGA TGGAAAACCA 
GAAAATGAGT TTGCGTTTAA TGCAGAATTT AAAGATAAGG ACTTTGCCAT TGATATTATT 
AAAAGCACTC ATGACCATTG GAAAGCATTA GTGACTAAGA AAACGAATGG AAAAGGAATC 
AGTTGCATGA ATACAACTTT GTCTGAGAGC CCCTTCAAGT GTGATCCTGA TGCTGCCAGA 
GCCATTGTGG ATGCTTTACC ACCACCCTGT GAATCTGCCT GCACAGTACC AACAGACGTG 
GATAAGTGGT TCCATCACCA GAAAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066952.1
CDS	103..972
Translation	MSGFSTEERAAPFSLEYRVFLKNEKGQYISPFHDIPIYADKDVFHMVVEVPRWSNAKMEIATKDPLNPIKQDVKKGKLRYVANLFPYKGYIWNYGAIPQTWEDPGHNDKHTGCCGDNDPIDVCEIGSKVCARGEIIGVKVLGILAMIDEGETDWKVIAINVDDPDAANYNDINDVKRLKPGYLEATVDWFRRYKVPDGKPENEFAFNAEFKDKDFAIDIIKSTHDHWKALVTKKTNGKGISCMNTTLSESPFKCDPDAARAIVDALPPPCESACTVPTDVDKWFHHQKN

Target ORF information:

RefSeq Version	NM_021129.4
Organism	Homo sapiens(human)
Definition	Homo sapiens inorganic pyrophosphatase 1 (PPA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021129.4

ORF Insert Sequence:

ATGAGCGGCT TCAGCACCGA GGAGCGCGCC GCGCCCTTCT CCCTGGAGTA CCGAGTCTTC 
CTCAAAAATG AGAAAGGACA ATATATATCT CCATTTCATG ATATTCCAAT TTATGCAGAT 
AAGGATGTGT TTCACATGGT AGTTGAAGTA CCACGCTGGT CTAATGCAAA AATGGAGATT 
GCTACAAAGG ACCCTTTAAA CCCTATTAAA CAAGATGTGA AAAAAGGAAA ACTTCGCTAT 
GTTGCGAATT TGTTCCCGTA TAAAGGATAT ATCTGGAACT ATGGTGCCAT CCCTCAGACT 
TGGGAAGACC CAGGGCACAA TGATAAACAT ACTGGCTGTT GTGGTGACAA TGACCCAATT 
GATGTGTGTG AAATTGGAAG CAAGGTATGT GCAAGAGGTG AAATAATTGG CGTGAAAGTT 
CTAGGCATAT TGGCTATGAT TGACGAAGGG GAAACCGACT GGAAAGTCAT TGCCATTAAT 
GTGGATGATC CTGATGCAGC CAATTATAAT GATATCAATG ATGTCAAACG GCTGAAACCT 
GGCTACTTAG AAGCTACTGT GGACTGGTTT AGAAGGTATA AGGTTCCTGA TGGAAAACCA 
GAAAATGAGT TTGCGTTTAA TGCAGAATTT AAAGATAAGG ACTTTGCCAT TGATATTATT 
AAAAGCACTC ATGACCATTG GAAAGCATTA GTGACTAAGA AAACGAATGG AAAAGGAATC 
AGTTGCATGA ATACAACTTT GTCTGAGAGC CCCTTCAAGT GTGATCCTGA TGCTGCCAGA 
GCCATTGTGG ATGCTTTACC ACCACCCTGT GAATCTGCCT GCACAGTACC AACAGACGTG 
GATAAGTGGT TCCATCACCA GAAAAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

p37/UBXN2B regulates spindle orientation by limiting cortical NuMA recruitment via PP1/Repo-Man.
The Journal of cell biology(2017 Dec)
Lee BH,Schwager F,Meraldi P,Gotta M

Expression of Inorganic Pyrophosphatase (PPA1) Correlates with Poor Prognosis of Epithelial Ovarian Cancer.
The Tohoku journal of experimental medicine241(2)165-173(2017 02)
Li H,Xiao N,Li Z,Wang Q

Clinical significance and functional validation of PPA1 in various tumors.
Cancer medicine5(10)2800-2812(2016 Oct)
Luo D,Wang G,Shen W,Zhao S,Zhou W,Wan L,Yuan L,Yang S,Xiang R