VPS36 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	VPS36
Entrez Gene ID	51028
Full Name	vacuolar protein sorting 36 homolog
Synonyms	C13orf9,CGI-145,EAP45
General protein information	Preferred Names vacuolar protein sorting 36 homolog Names vacuolar protein-sorting-associated protein 36 ELL-associated protein of 45 kDa ESCRT-II complex subunit VPS36
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 13 Map Location: 13q14.3
Summary	This gene encodes a protein that is a subunit of the endosomal sorting complex required for transport II (ESCRT-II). This protein complex functions in sorting of ubiquitinated membrane proteins during endocytosis. A similar protein complex in rat is associated with RNA polymerase elongation factor II. [provided by RefSeq, Aug 2013].
Disorder MIM:	610903

mRNA and Protein(s)

mRNA	Protein	Name
NM_016075.3	NP_057159.2	vacuolar protein-sorting-associated protein 36 isoform 1
NM_001282168.2	NP_001269097.1	vacuolar protein-sorting-associated protein 36 isoform 2
NM_001282169.1	NP_001269098.1	vacuolar protein-sorting-associated protein 36 isoform 3
NM_001282168.1	NP_001269097.1	vacuolar protein-sorting-associated protein 36 isoform 2
NM_016075.4	NP_057159.2	vacuolar protein-sorting-associated protein 36 isoform 1

Pathways from BioSystems

Homologies

	VPS36	XP_001082759.1
Xenopus tropicalis (tropical clawed frog)	vps36	NP_001016469.1
Mus musculus (house mouse)	Vps36	NP_081614.1
Drosophila melanogaster (fruit fly)	Vps36	NP_648660.1
Saccharomyces cerevisiae (baker's yeast)	VPS36	NP_013521.3
Kluyveromyces lactis	KLLA0B02541g	XP_451645.1
Magnaporthe oryzae (rice blast fungus)	MGG_01704	XP_003714699.1
Pan troglodytes (chimpanzee)	VPS36	XP_509796.2
Bos taurus (cattle)	VPS36	NP_001092409.1
Rattus norvegicus (Norway rat)	Vps36	NP_001099562.2
Gallus gallus (chicken)	VPS36	XP_417077.2
Caenorhabditis elegans (roundworm)	vps-36	NP_505798.2
Eremothecium gossypii	AGOS_AER092W	NP_984952.1
Homo sapiens (human)	VPS36	NP_057159.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004755	XP_554602.2
Neurospora crassa	NCU06595	XP_962337.2
Canis lupus familiaris (dog)	VPS36	XP_534484.2
Danio rerio (zebrafish)	vps36	NP_956271.1
Schizosaccharomyces pombe (fission yeast)	vps36	NP_596667.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

RAB27A, RAB27B and VPS36 are downregulated in advanced prostate cancer and show functional relevance in prostate cancer cells.
Worst TS, Meyer Y, Gottschalt M, Weis CA, von Hardenberg J, Frank C, Steidler A, Michel MS, Erben P
International journal of oncology50(3)920-932(2017 Mar)

A protein interaction network for Ecm29 links the 26 S proteasome to molecular motors and endosomal components.
Gorbea C, Pratt G, Ustrell V, Bell R, Sahasrabudhe S, Hughes RE, Rechsteiner M
The Journal of biological chemistry285(41)31616-33(2010 Oct)

Membrane budding and scission by the ESCRT machinery: it's all in the neck.
Hurley JH, Hanson PI
Nature reviews. Molecular cell biology11(8)556-66(2010 Aug)

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RAB27A, RAB27B and VPS36 are frequently underexpressed in advanced prostate cancer and are inversely correlated with prostate cancer outcome. There seems to be a close relationship in the expression of RAB27A, RAB27B and VPS36, with RAB27A and RAB27B being dependent on VPS36.
Title: RAB27A, RAB27B and VPS36 are downregulated in advanced prostate cancer and show functional relevance in prostate cancer cells.

Crystallographic and biochemical analyses reveal that the GLUE domain of the human ESCRT-II EAP45 (also called VPS36) subunit is a split pleckstrin-homology domain that binds ubiquitin along one edge of the beta-sandwich.
Title: Structural basis for ubiquitin recognition by the human ESCRT-II EAP45 GLUE domain.

mammalian ESCRTII is made up of hVps25p, hVps22p, and hVps36p and may be redundant, cargo-specific, or not required for protein sorting at the multivesicular body
Title: Degradation of endocytosed epidermal growth factor and virally ubiquitinated major histocompatibility complex class I is independent of mammalian ESCRTII.

The following VPS36 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS36 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu07780	NM_001282169.1 Latest version!	Homo sapiens vacuolar protein sorting 36 homolog (VPS36), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu10174	NM_016075.4 Latest version!	Homo sapiens vacuolar protein sorting 36 homolog (VPS36), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu01866	NM_001282168.2 Latest version!	Homo sapiens vacuolar protein sorting 36 homolog (VPS36), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu01866	NM_001282168.1	Homo sapiens vacuolar protein sorting 36 homolog (VPS36), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu10174	NM_016075.3	Homo sapiens vacuolar protein sorting 36 homolog (VPS36), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VPS36 ( NM_001282169.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269098.1 Homo sapiens vacuolar protein sorting 36 homolog (VPS36), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu07780
Clone ID Related Accession (Same CDS sequence)	NM_001282169.1
Accession Version	NM_001282169.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	vacuolar protein-sorting-associated protein 36 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AI221133.1, AL359513.12 and AI352102.1. On Aug 22, 2013 this sequence version replaced XM_005266410.1. Transcript Variant: This variant (3) contains an alternate 5' terminal exon, lacks a portion of the 5' coding region and initiates translation at a downstream start codon compared to variant 1. The encoded isoform (3) has a shorter N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.102814.1, SRR1660807.46746.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCATTC TCCTTTCCCA AATTGTGTTC ATTGAAGAAC AGGCGGCTGG AATTGGGAAG 
AGTGCCAAAA TAGTGGTTCA TCTTCACCCA GCTCCTCCTA ACAAAGAACC TGGCCCATTC 
CAGAGTAGTA AGAACTCCTA CATCAAACTC TCCTTCAAAG AACATGGCCA GATTGAGTTT 
TACAGGCGTT TATCAGAGGA AATGACACAA AGAAGATGGG AGAATATGCC AGTTTCCCAG 
TCATTACAAA CAAATAGAGG ACCCCAGCCA GGAAGAATAA GGGCTGTAGG AATTGTAGGT 
ATTGAAAGGA AACTGGAAGA AAAAAGAAAA GAAACTGACA AAAACATTTC TGAGGCCTTT 
GAAGACCTCA GCAAACTAAT GATCAAGGCT AAGGAAATGG TGGAATTATC AAAATCAATT 
GCTAATAAAA TTAAAGACAA ACAAGGTGAC ATCACAGAAG ATGAGACCAT CAGGTTTAAA 
TCCTACTTGC TGAGCATGGG AATAGCTAAC CCAGTTACCA GAGAAACCTA CGGCTCAGGC 
ACACAGTACC ACATGCAGCT GGCCAAACAA CTGGCTGGAA TATTGCAGGT GCCTTTAGAG 
GAACGAGGGG GAATAATGTC ACTCACGGAG GTGTACTGCT TAGTAAACCG AGCTCGAGGA 
ATGGAATTGC TCTCACCAGA AGATTTAGTG AATGCGTGCA AGATGCTGGA AGCACTGAAA 
TTACCTCTCA GGCTCCGTGT GTTTGACAGT GGCGTCATGG TAATTGAGCT TCAGTCTCAC 
AAGGAAGAGG AAATGGTGGC CTCGGCCCTG GAGACAGTTT CAGAAAAGGG ATCCCTAACA 
TCAGAAGAGT TTGCTAAGCT TGTGGGAATG TCTGTCCTCC TAGCCAAAGA AAGGTTGCTG 
CTTGCAGAGA AGATGGGCCA TCTTTGCCGT GATGACTCAG TGGAAGGCCT GCGTTTTTAC 
CCAAATTTAT TTATGACACA GAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269098.1
CDS	393..1379
Translation	MAILLSQIVFIEEQAAGIGKSAKIVVHLHPAPPNKEPGPFQSSKNSYIKLSFKEHGQIEFYRRLSEEMTQRRWENMPVSQSLQTNRGPQPGRIRAVGIVGIERKLEEKRKETDKNISEAFEDLSKLMIKAKEMVELSKSIANKIKDKQGDITEDETIRFKSYLLSMGIANPVTRETYGSGTQYHMQLAKQLAGILQVPLEERGGIMSLTEVYCLVNRARGMELLSPEDLVNACKMLEALKLPLRLRVFDSGVMVIELQSHKEEEMVASALETVSEKGSLTSEEFAKLVGMSVLLAKERLLLAEKMGHLCRDDSVEGLRFYPNLFMTQS

Target ORF information:

RefSeq Version	NM_001282169.1
Organism	Homo sapiens(human)
Definition	Homo sapiens vacuolar protein sorting 36 homolog (VPS36), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282169.1

ORF Insert Sequence:

ATGGCCATTC TCCTTTCCCA AATTGTGTTC ATTGAAGAAC AGGCGGCTGG AATTGGGAAG 
AGTGCCAAAA TAGTGGTTCA TCTTCACCCA GCTCCTCCTA ACAAAGAACC TGGCCCATTC 
CAGAGTAGTA AGAACTCCTA CATCAAACTC TCCTTCAAAG AACATGGCCA GATTGAGTTT 
TACAGGCGTT TATCAGAGGA AATGACACAA AGAAGATGGG AGAATATGCC AGTTTCCCAG 
TCATTACAAA CAAATAGAGG ACCCCAGCCA GGAAGAATAA GGGCTGTAGG AATTGTAGGT 
ATTGAAAGGA AACTGGAAGA AAAAAGAAAA GAAACTGACA AAAACATTTC TGAGGCCTTT 
GAAGACCTCA GCAAACTAAT GATCAAGGCT AAGGAAATGG TGGAATTATC AAAATCAATT 
GCTAATAAAA TTAAAGACAA ACAAGGTGAC ATCACAGAAG ATGAGACCAT CAGGTTTAAA 
TCCTACTTGC TGAGCATGGG AATAGCTAAC CCAGTTACCA GAGAAACCTA CGGCTCAGGC 
ACACAGTACC ACATGCAGCT GGCCAAACAA CTGGCTGGAA TATTGCAGGT GCCTTTAGAG 
GAACGAGGGG GAATAATGTC ACTCACGGAG GTGTACTGCT TAGTAAACCG AGCTCGAGGA 
ATGGAATTGC TCTCACCAGA AGATTTAGTG AATGCGTGCA AGATGCTGGA AGCACTGAAA 
TTACCTCTCA GGCTCCGTGT GTTTGACAGT GGCGTCATGG TAATTGAGCT TCAGTCTCAC 
AAGGAAGAGG AAATGGTGGC CTCGGCCCTG GAGACAGTTT CAGAAAAGGG ATCCCTAACA 
TCAGAAGAGT TTGCTAAGCT TGTGGGAATG TCTGTCCTCC TAGCCAAAGA AAGGTTGCTG 
CTTGCAGAGA AGATGGGCCA TCTTTGCCGT GATGACTCAG TGGAAGGCCT GCGTTTTTAC 
CCAAATTTAT TTATGACACA GAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS36 ( NM_016075.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_057159.2 Homo sapiens vacuolar protein sorting 36 homolog (VPS36), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu10174
Clone ID Related Accession (Same CDS sequence)	NM_016075.3 , NM_016075.4
Accession Version	NM_016075.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-09
Organism	Homo sapiens(human)
Product	vacuolar protein-sorting-associated protein 36 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA461148.1, AK289740.1, BC037279.1 and AI352102.1. On Aug 22, 2013 this sequence version replaced NM_016075.2. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.127893.1, SRR1660805.180542.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCGCT TCGTTTGGAC CAGCGGCCTC CTGGAGATCA ACGAGACCCT GGTGATCCAG 
CAGCGCGGGG TGCGAATCTA CGATGGCGAG GAGAAGATAA AATTTGATGC TGGGACTCTC 
CTTCTTAGTA CACACCGACT GATTTGGAGA GATCAGAAAA ATCATGAGTG TTGCATGGCC 
ATTCTCCTTT CCCAAATTGT GTTCATTGAA GAACAGGCGG CTGGAATTGG GAAGAGTGCC 
AAAATAGTGG TTCATCTTCA CCCAGCTCCT CCTAACAAAG AACCTGGCCC ATTCCAGAGT 
AGTAAGAACT CCTACATCAA ACTCTCCTTC AAAGAACATG GCCAGATTGA GTTTTACAGG 
CGTTTATCAG AGGAAATGAC ACAAAGAAGA TGGGAGAATA TGCCAGTTTC CCAGTCATTA 
CAAACAAATA GAGGACCCCA GCCAGGAAGA ATAAGGGCTG TAGGAATTGT AGGTATTGAA 
AGGAAACTGG AAGAAAAAAG AAAAGAAACT GACAAAAACA TTTCTGAGGC CTTTGAAGAC 
CTCAGCAAAC TAATGATCAA GGCTAAGGAA ATGGTGGAAT TATCAAAATC AATTGCTAAT 
AAAATTAAAG ACAAACAAGG TGACATCACA GAAGATGAGA CCATCAGGTT TAAATCCTAC 
TTGCTGAGCA TGGGAATAGC TAACCCAGTT ACCAGAGAAA CCTACGGCTC AGGCACACAG 
TACCACATGC AGCTGGCCAA ACAACTGGCT GGAATATTGC AGGTGCCTTT AGAGGAACGA 
GGGGGAATAA TGTCACTCAC GGAGGTGTAC TGCTTAGTAA ACCGAGCTCG AGGAATGGAA 
TTGCTCTCAC CAGAAGATTT AGTGAATGCG TGCAAGATGC TGGAAGCACT GAAATTACCT 
CTCAGGCTCC GTGTGTTTGA CAGTGGCGTC ATGGTAATTG AGCTTCAGTC TCACAAGGAA 
GAGGAAATGG TGGCCTCGGC CCTGGAGACA GTTTCAGAAA AGGGATCCCT AACATCAGAA 
GAGTTTGCTA AGCTTGTGGG AATGTCTGTC CTCCTAGCCA AAGAAAGGTT GCTGCTTGCA 
GAGAAGATGG GCCATCTTTG CCGTGATGAC TCAGTGGAAG GCCTGCGTTT TTACCCAAAT 
TTATTTATGA CACAGAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057159.2
CDS	85..1245
Translation	MDRFVWTSGLLEINETLVIQQRGVRIYDGEEKIKFDAGTLLLSTHRLIWRDQKNHECCMAILLSQIVFIEEQAAGIGKSAKIVVHLHPAPPNKEPGPFQSSKNSYIKLSFKEHGQIEFYRRLSEEMTQRRWENMPVSQSLQTNRGPQPGRIRAVGIVGIERKLEEKRKETDKNISEAFEDLSKLMIKAKEMVELSKSIANKIKDKQGDITEDETIRFKSYLLSMGIANPVTRETYGSGTQYHMQLAKQLAGILQVPLEERGGIMSLTEVYCLVNRARGMELLSPEDLVNACKMLEALKLPLRLRVFDSGVMVIELQSHKEEEMVASALETVSEKGSLTSEEFAKLVGMSVLLAKERLLLAEKMGHLCRDDSVEGLRFYPNLFMTQS

Target ORF information:

RefSeq Version	NM_016075.3
Organism	Homo sapiens(human)
Definition	Homo sapiens vacuolar protein sorting 36 homolog (VPS36), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016075.3

ORF Insert Sequence:

ATGGACCGCT TCGTTTGGAC CAGCGGCCTC CTGGAGATCA ACGAGACCCT GGTGATCCAG 
CAGCGCGGGG TGCGAATCTA CGATGGCGAG GAGAAGATAA AATTTGATGC TGGGACTCTC 
CTTCTTAGTA CACACCGACT GATTTGGAGA GATCAGAAAA ATCATGAGTG TTGCATGGCC 
ATTCTCCTTT CCCAAATTGT GTTCATTGAA GAACAGGCGG CTGGAATTGG GAAGAGTGCC 
AAAATAGTGG TTCATCTTCA CCCAGCTCCT CCTAACAAAG AACCTGGCCC ATTCCAGAGT 
AGTAAGAACT CCTACATCAA ACTCTCCTTC AAAGAACATG GCCAGATTGA GTTTTACAGG 
CGTTTATCAG AGGAAATGAC ACAAAGAAGA TGGGAGAATA TGCCAGTTTC CCAGTCATTA 
CAAACAAATA GAGGACCCCA GCCAGGAAGA ATAAGGGCTG TAGGAATTGT AGGTATTGAA 
AGGAAACTGG AAGAAAAAAG AAAAGAAACT GACAAAAACA TTTCTGAGGC CTTTGAAGAC 
CTCAGCAAAC TAATGATCAA GGCTAAGGAA ATGGTGGAAT TATCAAAATC AATTGCTAAT 
AAAATTAAAG ACAAACAAGG TGACATCACA GAAGATGAGA CCATCAGGTT TAAATCCTAC 
TTGCTGAGCA TGGGAATAGC TAACCCAGTT ACCAGAGAAA CCTACGGCTC AGGCACACAG 
TACCACATGC AGCTGGCCAA ACAACTGGCT GGAATATTGC AGGTGCCTTT AGAGGAACGA 
GGGGGAATAA TGTCACTCAC GGAGGTGTAC TGCTTAGTAA ACCGAGCTCG AGGAATGGAA 
TTGCTCTCAC CAGAAGATTT AGTGAATGCG TGCAAGATGC TGGAAGCACT GAAATTACCT 
CTCAGGCTCC GTGTGTTTGA CAGTGGCGTC ATGGTAATTG AGCTTCAGTC TCACAAGGAA 
GAGGAAATGG TGGCCTCGGC CCTGGAGACA GTTTCAGAAA AGGGATCCCT AACATCAGAA 
GAGTTTGCTA AGCTTGTGGG AATGTCTGTC CTCCTAGCCA AAGAAAGGTT GCTGCTTGCA 
GAGAAGATGG GCCATCTTTG CCGTGATGAC TCAGTGGAAG GCCTGCGTTT TTACCCAAAT 
TTATTTATGA CACAGAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS36 ( NM_016075.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_057159.2 Homo sapiens vacuolar protein sorting 36 homolog (VPS36), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu10174
Clone ID Related Accession (Same CDS sequence)	NM_016075.3 , NM_016075.4
Accession Version	NM_016075.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-23
Organism	Homo sapiens(human)
Product	vacuolar protein-sorting-associated protein 36 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA461148.1, AK289740.1, BC037279.1 and AI352102.1. On Nov 22, 2018 this sequence version replaced NM_016075.3. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.16788.1, SRR1660807.127893.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000378060.9/ ENSP00000367299.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGACCGCT TCGTTTGGAC CAGCGGCCTC CTGGAGATCA ACGAGACCCT GGTGATCCAG 
CAGCGCGGGG TGCGAATCTA CGATGGCGAG GAGAAGATAA AATTTGATGC TGGGACTCTC 
CTTCTTAGTA CACACCGACT GATTTGGAGA GATCAGAAAA ATCATGAGTG TTGCATGGCC 
ATTCTCCTTT CCCAAATTGT GTTCATTGAA GAACAGGCGG CTGGAATTGG GAAGAGTGCC 
AAAATAGTGG TTCATCTTCA CCCAGCTCCT CCTAACAAAG AACCTGGCCC ATTCCAGAGT 
AGTAAGAACT CCTACATCAA ACTCTCCTTC AAAGAACATG GCCAGATTGA GTTTTACAGG 
CGTTTATCAG AGGAAATGAC ACAAAGAAGA TGGGAGAATA TGCCAGTTTC CCAGTCATTA 
CAAACAAATA GAGGACCCCA GCCAGGAAGA ATAAGGGCTG TAGGAATTGT AGGTATTGAA 
AGGAAACTGG AAGAAAAAAG AAAAGAAACT GACAAAAACA TTTCTGAGGC CTTTGAAGAC 
CTCAGCAAAC TAATGATCAA GGCTAAGGAA ATGGTGGAAT TATCAAAATC AATTGCTAAT 
AAAATTAAAG ACAAACAAGG TGACATCACA GAAGATGAGA CCATCAGGTT TAAATCCTAC 
TTGCTGAGCA TGGGAATAGC TAACCCAGTT ACCAGAGAAA CCTACGGCTC AGGCACACAG 
TACCACATGC AGCTGGCCAA ACAACTGGCT GGAATATTGC AGGTGCCTTT AGAGGAACGA 
GGGGGAATAA TGTCACTCAC GGAGGTGTAC TGCTTAGTAA ACCGAGCTCG AGGAATGGAA 
TTGCTCTCAC CAGAAGATTT AGTGAATGCG TGCAAGATGC TGGAAGCACT GAAATTACCT 
CTCAGGCTCC GTGTGTTTGA CAGTGGCGTC ATGGTAATTG AGCTTCAGTC TCACAAGGAA 
GAGGAAATGG TGGCCTCGGC CCTGGAGACA GTTTCAGAAA AGGGATCCCT AACATCAGAA 
GAGTTTGCTA AGCTTGTGGG AATGTCTGTC CTCCTAGCCA AAGAAAGGTT GCTGCTTGCA 
GAGAAGATGG GCCATCTTTG CCGTGATGAC TCAGTGGAAG GCCTGCGTTT TTACCCAAAT 
TTATTTATGA CACAGAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057159.2
CDS	41..1201
Translation	MDRFVWTSGLLEINETLVIQQRGVRIYDGEEKIKFDAGTLLLSTHRLIWRDQKNHECCMAILLSQIVFIEEQAAGIGKSAKIVVHLHPAPPNKEPGPFQSSKNSYIKLSFKEHGQIEFYRRLSEEMTQRRWENMPVSQSLQTNRGPQPGRIRAVGIVGIERKLEEKRKETDKNISEAFEDLSKLMIKAKEMVELSKSIANKIKDKQGDITEDETIRFKSYLLSMGIANPVTRETYGSGTQYHMQLAKQLAGILQVPLEERGGIMSLTEVYCLVNRARGMELLSPEDLVNACKMLEALKLPLRLRVFDSGVMVIELQSHKEEEMVASALETVSEKGSLTSEEFAKLVGMSVLLAKERLLLAEKMGHLCRDDSVEGLRFYPNLFMTQS

Target ORF information:

RefSeq Version	NM_016075.4
Organism	Homo sapiens(human)
Definition	Homo sapiens vacuolar protein sorting 36 homolog (VPS36), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016075.4

ORF Insert Sequence:

ATGGACCGCT TCGTTTGGAC CAGCGGCCTC CTGGAGATCA ACGAGACCCT GGTGATCCAG 
CAGCGCGGGG TGCGAATCTA CGATGGCGAG GAGAAGATAA AATTTGATGC TGGGACTCTC 
CTTCTTAGTA CACACCGACT GATTTGGAGA GATCAGAAAA ATCATGAGTG TTGCATGGCC 
ATTCTCCTTT CCCAAATTGT GTTCATTGAA GAACAGGCGG CTGGAATTGG GAAGAGTGCC 
AAAATAGTGG TTCATCTTCA CCCAGCTCCT CCTAACAAAG AACCTGGCCC ATTCCAGAGT 
AGTAAGAACT CCTACATCAA ACTCTCCTTC AAAGAACATG GCCAGATTGA GTTTTACAGG 
CGTTTATCAG AGGAAATGAC ACAAAGAAGA TGGGAGAATA TGCCAGTTTC CCAGTCATTA 
CAAACAAATA GAGGACCCCA GCCAGGAAGA ATAAGGGCTG TAGGAATTGT AGGTATTGAA 
AGGAAACTGG AAGAAAAAAG AAAAGAAACT GACAAAAACA TTTCTGAGGC CTTTGAAGAC 
CTCAGCAAAC TAATGATCAA GGCTAAGGAA ATGGTGGAAT TATCAAAATC AATTGCTAAT 
AAAATTAAAG ACAAACAAGG TGACATCACA GAAGATGAGA CCATCAGGTT TAAATCCTAC 
TTGCTGAGCA TGGGAATAGC TAACCCAGTT ACCAGAGAAA CCTACGGCTC AGGCACACAG 
TACCACATGC AGCTGGCCAA ACAACTGGCT GGAATATTGC AGGTGCCTTT AGAGGAACGA 
GGGGGAATAA TGTCACTCAC GGAGGTGTAC TGCTTAGTAA ACCGAGCTCG AGGAATGGAA 
TTGCTCTCAC CAGAAGATTT AGTGAATGCG TGCAAGATGC TGGAAGCACT GAAATTACCT 
CTCAGGCTCC GTGTGTTTGA CAGTGGCGTC ATGGTAATTG AGCTTCAGTC TCACAAGGAA 
GAGGAAATGG TGGCCTCGGC CCTGGAGACA GTTTCAGAAA AGGGATCCCT AACATCAGAA 
GAGTTTGCTA AGCTTGTGGG AATGTCTGTC CTCCTAGCCA AAGAAAGGTT GCTGCTTGCA 
GAGAAGATGG GCCATCTTTG CCGTGATGAC TCAGTGGAAG GCCTGCGTTT TTACCCAAAT 
TTATTTATGA CACAGAGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS36 ( NM_001282168.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269097.1 Homo sapiens vacuolar protein sorting 36 homolog (VPS36), transcript variant 2, mRNA.

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CloneID	OHu01866
Clone ID Related Accession (Same CDS sequence)	NM_001282168.2 , NM_001282168.1
Accession Version	NM_001282168.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	vacuolar protein-sorting-associated protein 36 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA461148.1, BX647847.1, CB959894.1, AL359513.12 and AI352102.1. On May 31, 2019 this sequence version replaced NM_001282168.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' terminal exon compared to variant 1. This results in a shorter protein (isoform 2) compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX647847.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCGCT TCGTTTGGAC CAGCGGCCTC CTGGAGATCA ACGAGACCCT GGTGATCCAG 
CAGCGCGGGA TAAAATTTGA TGCTGGGACT CTCCTTCTTA GTACACACCG ACTGATTTGG 
AGAGATCAGA AAAATCATGA GTGTTGCATG GCCATTCTCC TTTCCCAAAT TGTGTTCATT 
GAAGAACAGG CGGCTGGAAT TGGGAAGAGT GCCAAAATAG TGGTTCATCT TCACCCAGCT 
CCTCCTAACA AAGAACCTGG CCCATTCCAG AGTAGTAAGA ACTCCTACAT CAAACTCTCC 
TTCAAAGAAC ATGGCCAGAT TGAGTTTTAC AGGCGTTTAT CAGAGGAAAT GACACAAAGA 
AGATGGGAGA ATATGCCAGT TTCCCAGTCA TTACAAACAA ATAGAGGACC CCAGCCAGGA 
AGAATAAGGG CTGTAGGAAT TGTAGGTATT GAAAGGAAAC TGGAAGAAAA AAGAAAAGAA 
ACTGACAAAA ACATTTCTGA GGCCTTTGAA GACCTCAGCA AACTAATGAT CAAGGCTAAG 
GAAATGGTGG AATTATCAAA ATCAATTGCT AATAAAATTA AAGACAAACA AGGTGACATC 
ACAGAAGATG AGACCATCAG GTTTAAATCC TACTTGCTGA GCATGGGAAT AGCTAACCCA 
GTTACCAGAG AAACCTACGG CTCAGGCACA CAGTACCACA TGCAGCTGGC CAAACAACTG 
GCTGGAATAT TGCAGGTGCC TTTAGAGGAA CGAGGGGGAA TAATGTCACT CACGGAGGTG 
TACTGCTTAG TAAACCGAGC TCGAGGAATG GAATTGCTCT CACCAGAAGA TTTAGTGAAT 
GCGTGCAAGA TGCTGGAAGC ACTGAAATTA CCTCTCAGGC TCCGTGTGTT TGACAGTGGC 
GTCATGGTAA TTGAGCTTCA GTCTCACAAG GAAGAGGAAA TGGTGGCCTC GGCCCTGGAG 
ACAGTTTCAG AAAAGGGATC CCTAACATCA GAAGAGTTTG CTAAGCTTGT GGGAATGTCT 
GTCCTCCTAG CCAAAGAAAG GTTGCTGCTT GCAGAGAAGA TGGGCCATCT TTGCCGTGAT 
GACTCAGTGG AAGGCCTGCG TTTTTACCCA AATTTATTTA TGACACAGAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269097.1
CDS	41..1174
Translation	MDRFVWTSGLLEINETLVIQQRGIKFDAGTLLLSTHRLIWRDQKNHECCMAILLSQIVFIEEQAAGIGKSAKIVVHLHPAPPNKEPGPFQSSKNSYIKLSFKEHGQIEFYRRLSEEMTQRRWENMPVSQSLQTNRGPQPGRIRAVGIVGIERKLEEKRKETDKNISEAFEDLSKLMIKAKEMVELSKSIANKIKDKQGDITEDETIRFKSYLLSMGIANPVTRETYGSGTQYHMQLAKQLAGILQVPLEERGGIMSLTEVYCLVNRARGMELLSPEDLVNACKMLEALKLPLRLRVFDSGVMVIELQSHKEEEMVASALETVSEKGSLTSEEFAKLVGMSVLLAKERLLLAEKMGHLCRDDSVEGLRFYPNLFMTQS

Target ORF information:

RefSeq Version	NM_001282168.2
Organism	Homo sapiens(human)
Definition	Homo sapiens vacuolar protein sorting 36 homolog (VPS36), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282168.2

ORF Insert Sequence:

ATGGACCGCT TCGTTTGGAC CAGCGGCCTC CTGGAGATCA ACGAGACCCT GGTGATCCAG 
CAGCGCGGGA TAAAATTTGA TGCTGGGACT CTCCTTCTTA GTACACACCG ACTGATTTGG 
AGAGATCAGA AAAATCATGA GTGTTGCATG GCCATTCTCC TTTCCCAAAT TGTGTTCATT 
GAAGAACAGG CGGCTGGAAT TGGGAAGAGT GCCAAAATAG TGGTTCATCT TCACCCAGCT 
CCTCCTAACA AAGAACCTGG CCCATTCCAG AGTAGTAAGA ACTCCTACAT CAAACTCTCC 
TTCAAAGAAC ATGGCCAGAT TGAGTTTTAC AGGCGTTTAT CAGAGGAAAT GACACAAAGA 
AGATGGGAGA ATATGCCAGT TTCCCAGTCA TTACAAACAA ATAGAGGACC CCAGCCAGGA 
AGAATAAGGG CTGTAGGAAT TGTAGGTATT GAAAGGAAAC TGGAAGAAAA AAGAAAAGAA 
ACTGACAAAA ACATTTCTGA GGCCTTTGAA GACCTCAGCA AACTAATGAT CAAGGCTAAG 
GAAATGGTGG AATTATCAAA ATCAATTGCT AATAAAATTA AAGACAAACA AGGTGACATC 
ACAGAAGATG AGACCATCAG GTTTAAATCC TACTTGCTGA GCATGGGAAT AGCTAACCCA 
GTTACCAGAG AAACCTACGG CTCAGGCACA CAGTACCACA TGCAGCTGGC CAAACAACTG 
GCTGGAATAT TGCAGGTGCC TTTAGAGGAA CGAGGGGGAA TAATGTCACT CACGGAGGTG 
TACTGCTTAG TAAACCGAGC TCGAGGAATG GAATTGCTCT CACCAGAAGA TTTAGTGAAT 
GCGTGCAAGA TGCTGGAAGC ACTGAAATTA CCTCTCAGGC TCCGTGTGTT TGACAGTGGC 
GTCATGGTAA TTGAGCTTCA GTCTCACAAG GAAGAGGAAA TGGTGGCCTC GGCCCTGGAG 
ACAGTTTCAG AAAAGGGATC CCTAACATCA GAAGAGTTTG CTAAGCTTGT GGGAATGTCT 
GTCCTCCTAG CCAAAGAAAG GTTGCTGCTT GCAGAGAAGA TGGGCCATCT TTGCCGTGAT 
GACTCAGTGG AAGGCCTGCG TTTTTACCCA AATTTATTTA TGACACAGAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS36 ( NM_001282168.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001269097.1 Homo sapiens vacuolar protein sorting 36 homolog (VPS36), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu01866
Clone ID Related Accession (Same CDS sequence)	NM_001282168.2 , NM_001282168.1
Accession Version	NM_001282168.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	vacuolar protein-sorting-associated protein 36 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA461148.1, BX647847.1, CB959894.1, AL359513.12 and AI352102.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' terminal exon compared to variant 1. This results in a shorter protein (isoform 2) compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX647847.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCGCT TCGTTTGGAC CAGCGGCCTC CTGGAGATCA ACGAGACCCT GGTGATCCAG 
CAGCGCGGGA TAAAATTTGA TGCTGGGACT CTCCTTCTTA GTACACACCG ACTGATTTGG 
AGAGATCAGA AAAATCATGA GTGTTGCATG GCCATTCTCC TTTCCCAAAT TGTGTTCATT 
GAAGAACAGG CGGCTGGAAT TGGGAAGAGT GCCAAAATAG TGGTTCATCT TCACCCAGCT 
CCTCCTAACA AAGAACCTGG CCCATTCCAG AGTAGTAAGA ACTCCTACAT CAAACTCTCC 
TTCAAAGAAC ATGGCCAGAT TGAGTTTTAC AGGCGTTTAT CAGAGGAAAT GACACAAAGA 
AGATGGGAGA ATATGCCAGT TTCCCAGTCA TTACAAACAA ATAGAGGACC CCAGCCAGGA 
AGAATAAGGG CTGTAGGAAT TGTAGGTATT GAAAGGAAAC TGGAAGAAAA AAGAAAAGAA 
ACTGACAAAA ACATTTCTGA GGCCTTTGAA GACCTCAGCA AACTAATGAT CAAGGCTAAG 
GAAATGGTGG AATTATCAAA ATCAATTGCT AATAAAATTA AAGACAAACA AGGTGACATC 
ACAGAAGATG AGACCATCAG GTTTAAATCC TACTTGCTGA GCATGGGAAT AGCTAACCCA 
GTTACCAGAG AAACCTACGG CTCAGGCACA CAGTACCACA TGCAGCTGGC CAAACAACTG 
GCTGGAATAT TGCAGGTGCC TTTAGAGGAA CGAGGGGGAA TAATGTCACT CACGGAGGTG 
TACTGCTTAG TAAACCGAGC TCGAGGAATG GAATTGCTCT CACCAGAAGA TTTAGTGAAT 
GCGTGCAAGA TGCTGGAAGC ACTGAAATTA CCTCTCAGGC TCCGTGTGTT TGACAGTGGC 
GTCATGGTAA TTGAGCTTCA GTCTCACAAG GAAGAGGAAA TGGTGGCCTC GGCCCTGGAG 
ACAGTTTCAG AAAAGGGATC CCTAACATCA GAAGAGTTTG CTAAGCTTGT GGGAATGTCT 
GTCCTCCTAG CCAAAGAAAG GTTGCTGCTT GCAGAGAAGA TGGGCCATCT TTGCCGTGAT 
GACTCAGTGG AAGGCCTGCG TTTTTACCCA AATTTATTTA TGACACAGAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269097.1
CDS	85..1218
Translation	MDRFVWTSGLLEINETLVIQQRGIKFDAGTLLLSTHRLIWRDQKNHECCMAILLSQIVFIEEQAAGIGKSAKIVVHLHPAPPNKEPGPFQSSKNSYIKLSFKEHGQIEFYRRLSEEMTQRRWENMPVSQSLQTNRGPQPGRIRAVGIVGIERKLEEKRKETDKNISEAFEDLSKLMIKAKEMVELSKSIANKIKDKQGDITEDETIRFKSYLLSMGIANPVTRETYGSGTQYHMQLAKQLAGILQVPLEERGGIMSLTEVYCLVNRARGMELLSPEDLVNACKMLEALKLPLRLRVFDSGVMVIELQSHKEEEMVASALETVSEKGSLTSEEFAKLVGMSVLLAKERLLLAEKMGHLCRDDSVEGLRFYPNLFMTQS

Target ORF information:

RefSeq Version	NM_001282168.1
Organism	Homo sapiens(human)
Definition	Homo sapiens vacuolar protein sorting 36 homolog (VPS36), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282168.1

ORF Insert Sequence:

ATGGACCGCT TCGTTTGGAC CAGCGGCCTC CTGGAGATCA ACGAGACCCT GGTGATCCAG 
CAGCGCGGGA TAAAATTTGA TGCTGGGACT CTCCTTCTTA GTACACACCG ACTGATTTGG 
AGAGATCAGA AAAATCATGA GTGTTGCATG GCCATTCTCC TTTCCCAAAT TGTGTTCATT 
GAAGAACAGG CGGCTGGAAT TGGGAAGAGT GCCAAAATAG TGGTTCATCT TCACCCAGCT 
CCTCCTAACA AAGAACCTGG CCCATTCCAG AGTAGTAAGA ACTCCTACAT CAAACTCTCC 
TTCAAAGAAC ATGGCCAGAT TGAGTTTTAC AGGCGTTTAT CAGAGGAAAT GACACAAAGA 
AGATGGGAGA ATATGCCAGT TTCCCAGTCA TTACAAACAA ATAGAGGACC CCAGCCAGGA 
AGAATAAGGG CTGTAGGAAT TGTAGGTATT GAAAGGAAAC TGGAAGAAAA AAGAAAAGAA 
ACTGACAAAA ACATTTCTGA GGCCTTTGAA GACCTCAGCA AACTAATGAT CAAGGCTAAG 
GAAATGGTGG AATTATCAAA ATCAATTGCT AATAAAATTA AAGACAAACA AGGTGACATC 
ACAGAAGATG AGACCATCAG GTTTAAATCC TACTTGCTGA GCATGGGAAT AGCTAACCCA 
GTTACCAGAG AAACCTACGG CTCAGGCACA CAGTACCACA TGCAGCTGGC CAAACAACTG 
GCTGGAATAT TGCAGGTGCC TTTAGAGGAA CGAGGGGGAA TAATGTCACT CACGGAGGTG 
TACTGCTTAG TAAACCGAGC TCGAGGAATG GAATTGCTCT CACCAGAAGA TTTAGTGAAT 
GCGTGCAAGA TGCTGGAAGC ACTGAAATTA CCTCTCAGGC TCCGTGTGTT TGACAGTGGC 
GTCATGGTAA TTGAGCTTCA GTCTCACAAG GAAGAGGAAA TGGTGGCCTC GGCCCTGGAG 
ACAGTTTCAG AAAAGGGATC CCTAACATCA GAAGAGTTTG CTAAGCTTGT GGGAATGTCT 
GTCCTCCTAG CCAAAGAAAG GTTGCTGCTT GCAGAGAAGA TGGGCCATCT TTGCCGTGAT 
GACTCAGTGG AAGGCCTGCG TTTTTACCCA AATTTATTTA TGACACAGAG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

RAB27A, RAB27B and VPS36 are downregulated in advanced prostate cancer and show functional relevance in prostate cancer cells.
International journal of oncology50(3)920-932(2017 Mar)
Worst TS,Meyer Y,Gottschalt M,Weis CA,von Hardenberg J,Frank C,Steidler A,Michel MS,Erben P

A protein interaction network for Ecm29 links the 26 S proteasome to molecular motors and endosomal components.
The Journal of biological chemistry285(41)31616-33(2010 Oct)
Gorbea C,Pratt G,Ustrell V,Bell R,Sahasrabudhe S,Hughes RE,Rechsteiner M

Membrane budding and scission by the ESCRT machinery: it's all in the neck.
Nature reviews. Molecular cell biology11(8)556-66(2010 Aug)
Hurley JH,Hanson PI

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)
Gonzales PA,Pisitkun T,Hoffert JD,Tchapyjnikov D,Star RA,Kleta R,Wang NS,Knepper MA

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VPS36 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

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電話

FAX

オンラインフォーム