ARHGEF4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ARHGEF4
Entrez Gene ID	50649
Full Name	Rho guanine nucleotide exchange factor 4
Synonyms	ASEF,ASEF1,GEF4,STM6
General protein information	Preferred Names Rho guanine nucleotide exchange factor 4 Names rho guanine nucleotide exchange factor 4 APC-stimulated guanine nucleotide exchange factor 1 Rho guanine nucleotide exchange factor (GEF) 4
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q21.1
Summary	Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The protein encoded by this gene may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants encoding different isoforms have been found, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jun 2013].
Disorder MIM:	605216

mRNA and Protein(s)

mRNA	Protein	Name
XM_011511274.2	XP_011509576.1	rho guanine nucleotide exchange factor 4 isoform X2
XM_005263685.3	XP_005263742.1	rho guanine nucleotide exchange factor 4 isoform X4
XM_005263687.4	XP_005263744.1	rho guanine nucleotide exchange factor 4 isoform X5
NM_001375900.1	NP_001362829.1	rho guanine nucleotide exchange factor 4 isoform d
XM_017004231.1	XP_016859720.1	rho guanine nucleotide exchange factor 4 isoform X3
XM_005263688.3	XP_005263745.1	rho guanine nucleotide exchange factor 4 isoform X6
NM_015320.3	NP_056135.2	rho guanine nucleotide exchange factor 4 isoform a
NM_001375901.1	NP_001362830.1	rho guanine nucleotide exchange factor 4 isoform e
NM_001375902.1	NP_001362831.1	rho guanine nucleotide exchange factor 4 isoform f
NM_001367493.1	NP_001354422.1	rho guanine nucleotide exchange factor 4 isoform c
NM_032995.2	NP_127462.1	rho guanine nucleotide exchange factor 4 isoform b
XM_005263689.4	XP_005263746.1	rho guanine nucleotide exchange factor 4 isoform X6
XM_011511277.2	XP_011509579.1	rho guanine nucleotide exchange factor 4 isoform X6
XM_011511277.1	XP_011509579.1	rho guanine nucleotide exchange factor 4 isoform X6
NM_015320.4	NP_056135.2	rho guanine nucleotide exchange factor 4 isoform a
NM_001375903.1	NP_001362832.1	rho guanine nucleotide exchange factor 4 isoform g
NM_001375904.1	NP_001362833.1	rho guanine nucleotide exchange factor 4 isoform h
XM_005263689.5	XP_005263746.1	rho guanine nucleotide exchange factor 4 isoform X6
XM_024452938.1	XP_024308706.1	rho guanine nucleotide exchange factor 4 isoform X6
XM_005263687.3	XP_005263744.1	rho guanine nucleotide exchange factor 4 isoform X5
XM_011511276.2	XP_011509578.1	rho guanine nucleotide exchange factor 4 isoform X6
XM_005263688.2	XP_005263745.1	rho guanine nucleotide exchange factor 4 isoform X6
XM_005263681.1	XP_005263738.1	rho guanine nucleotide exchange factor 4 isoform X1
XM_011511274.3	XP_011509576.1	rho guanine nucleotide exchange factor 4 isoform X2
XM_011511276.3	XP_011509578.1	rho guanine nucleotide exchange factor 4 isoform X6

Pathways from BioSystems

Homologies

Homo sapiens (human)	ARHGEF4	NP_056135.2
Xenopus tropicalis (tropical clawed frog)	LOC100487089	XP_004917856.1
Bos taurus (cattle)	ARHGEF4	NP_001179002.1
Rattus norvegicus (Norway rat)	Arhgef4	XP_006244788.1
Danio rerio (zebrafish)	arhgef4	XP_687404.3
Pan troglodytes (chimpanzee)	ARHGEF4	XP_525919.2
Mus musculus (house mouse)	Arhgef4	NP_898840.2
	LOC703647	XP_002808100.1
Canis lupus familiaris (dog)	LOC483856	XP_540976.4
Gallus gallus (chicken)	ARHGEF4	XP_004937023.1

Gene Ontology
Bibliography

Related articles in PubMed

Copy Number Variations in Candidate Genes and Intergenic Regions Affect Body Mass Index and Abdominal Obesity in Mexican Children.
Ant?nez-Ortiz DL, Flores-Alfaro E, Burguete-Garc?a AI, Bonnefond A, Peralta-Romero J, Froguel P, Espinoza-Rojo M, Cruz M
BioMed research international20172432957(2017)

Quantitative genome-wide methylation analysis of high-grade non-muscle invasive bladder cancer.
Kitchen MO, Bryan RT, Emes RD, Glossop JR, Luscombe C, Cheng KK, Zeegers MP, James ND, Devall AJ, Mein CA, Gommersall L, Fryer AA, Farrell WE
Epigenetics11(3)237-46(2016 03)

Asef mediates HGF protective effects against LPS-induced lung injury and endothelial barrier dysfunction.
Meng F, Meliton A, Moldobaeva N, Mutlu G, Kawasaki Y, Akiyama T, Birukova AA
American journal of physiology. Lung cellular and molecular physiology308(5)L452-63(2015 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Although inappropriate promoter methylation was not invariantly associated with reduced transcript expression, a significant association was apparent for the ARHGEF4, PON3, STAT5a, and VAX2 gene transcripts (P<0.05). Herein, we present the first genome-wide DNA methylation analysis in a unique HG-NMIBC cohort, showing extensive and discrete methylation changes relative to normal bladder and low-intermediate-grade tumor
Title: Quantitative genome-wide methylation analysis of high-grade non-muscle invasive bladder cancer.

these results demonstrate potent anti-inflammatory effects by HGF and delineate a key role of Asef in the mediation of the HGF barrier protective and anti-inflammatory effects.
Title: Asef mediates HGF protective effects against LPS-induced lung injury and endothelial barrier dysfunction.

These data demonstrate, that in addition to microtubule-independent Tiam1 activation, HGF engages additional microtubule- and APC-dependent pathway of Asef activation.
Title: Hepatocyte growth factor triggers distinct mechanisms of Asef and Tiam1 activation to induce endothelial barrier enhancement.

Expression of Asef was associated with lower estimated glomerular filtration rate in kidney disease.
Title: Association of Asef and Cdc42 expression to tubular injury in diseased human kidney.

Expression of ARHGEF4 (Rho guanine nucleotide exchange factor 4) is restricted to the brain and may regulate the actin cytoskeletal network, cell morphology and migration, and neuronal function
Title: Small rare recurrent deletions and reciprocal duplications in 2q21.1, including brain-specific ARHGEF4 and GPR148.

The following ARHGEF4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARHGEF4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu08729	NM_015320.4 Latest version!	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$344.50-$482.30 $689.00
OHu09786	NM_032995.2 Latest version!	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OHu32422	XM_011511277.2 Latest version!	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu32422	XM_005263688.3 Latest version!	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu32422	XM_011511276.3 Latest version!	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu32422	XM_024452938.1 Latest version!	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu32422	XM_005263689.5 Latest version!	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu36332	XM_005263687.4 Latest version!	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu109041	NM_001375900.1 Latest version!	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu109043	NM_001375901.1 Latest version!	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu109044	NM_001375903.1 Latest version!	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu109045	NM_001375902.1 Latest version!	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu36332	NM_001375904.1 Latest version!	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu36328	NM_001367493.1 Latest version!	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu36328	XM_005263681.1 Latest version!	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu36331	XM_005263685.3 Latest version!	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OHu67537	XM_011511274.3 Latest version!	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu89522	XM_017004231.1 Latest version!	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
View Old Accession Versions >>
OHu32422	XM_011511277.1	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu08729	NM_015320.3	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$344.50-$482.30 $689.00
OHu32422	XM_005263689.4	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu36332	XM_005263687.3	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu32422	XM_005263688.2	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu67537	XM_011511274.2	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu32422	XM_011511276.2	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ARHGEF4 ( NM_015320.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_056135.2 Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu08729
Clone ID Related Accession (Same CDS sequence)	NM_015320.3 , NM_015320.4
Accession Version	NM_015320.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 4 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC112786.4, AF249745.1, AB042199.1 and AL137289.1. On Jun 25, 2013 this sequence version replaced NM_015320.2. Transcript Variant: This variant (1) encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF249745.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145240, SAMEA2145313 [ECO:0000348] ##Evidence-Data-END##

ATGCCCTGGG AAGAACCAGC AGGTGAGAAG CCCAGTTGCT CTCACAGTCA GAAGGCGTTC 
CACATGGAGC CTGCCCAGAA GCCCTGCTTC ACCACTGACA TGGTGACATG GGCCCTCCTC 
TGCATCTCTG CAGAGACTGT GCGTGGGGAG GCTCCTTCAC AGCCTAGGGG CATCCCTCAC 
CGCTCGCCCG TCAGTGTGGA TGACCTGTGG CTGGAGAAGA CACAGAGAAA GAAGTTGCAG 
AAGCAGGCCC ACGTCGAAAG GAGGCTGCAC ATAGGGGCAG TGCACAAAGA TGGAGTCAAG 
TGCTGGAGAA AGACGATCAT TACCTCTCCA GAGTCTTTGA ATCTCCCTAG AAGAAGCCAT 
CCACTCTCCC AGAGTGCTCC AACGGGACTG AACCACATGG GCTGGCCAGA GCACACACCA 
GGCACTGCCA TGCCTGATGG AGCTCTGGAC ACAGCTGTCT GCGCTGACGA AGTGGGGAGC 
GAGGAGGACC TGTATGATGA CCTGCACAGC TCCAGCCACC ACTACAGCCA CCCTGGAGGG 
GGTGGGGAGC AGCTGGCTAT CAATGAGCTC ATCAGCGATG GCAGTGTGGT CTGCGCTGAA 
GCACTCTGGG ACCATGTCAC CATGGACGAC CAGGAGCTGG GCTTCAAAGC TGGGGACGTC 
ATCGAAGTGA TGGATGCCAC CAACAGAGAG TGGTGGTGGG GCCGGGTCGC CGATGGCGAG 
GGCTGGTTTC CAGCCAGCTT CGTTCGGCTG AGGGTGAATC AGGACGAGCC CGCGGATGAC 
GACGCCCCTC TGGCCGGGAA CAGCGGAGCG GAGGACGGCG GGGCGGAGGC GCAGAGCAGC 
AAGGACCAGA TGCGGACCAA CGTCATCAAC GAGATCCTCA GCACTGAGCG GGACTACATC 
AAGCACCTGC GCGACATCTG CGAGGGCTAC GTCCGGCAGT GCCGCAAGCG CGCAGACATG 
TTCAGCGAGG AGCAGCTGCG TACCATCTTC GGGAACATCG AGGACATCTA CCGCTGCCAG 
AAGGCCTTCG TGAAGGCCCT GGAGCAGAGG TTCAACCGCG AGCGCCCACA CCTGAGCGAG 
CTGGGTGCCT GCTTCCTGGA GCATCAAGCC GACTTCCAGA TCTACTCGGA GTACTGCAAT 
AACCACCCCA ACGCCTGCGT GGAGCTCTCC CGGCTCACCA AGCTCAGCAA GTACGTGTAC 
TTCTTCGAGG CCTGCCGGCT GCTGCAGAAG ATGATTGACA TCTCCCTGGA TGGCTTCCTG 
CTGACTCCGG TGCAGAAGAT CTGCAAGTAC CCTCTGCAGC TGGCCGAGCT GCTCAAATAC 
ACGCACCCCC AGCACAGGGA CTTCAAGGAT GTTGAAGCCG CCTTGCATGC CATGAAGAAC 
GTGGCCCAGC TCATCAACGA GCGGAAGCGG AGACTTGAGA ACATCGACAA GATTGCTCAG 
TGGCAGAGCT CCATAGAGGA CTGGGAGGGA GAAGATCTCT TGGTCAGGAG CTCAGAACTC 
ATCTACTCGG GGGAGCTGAC TCGAGTTACA CAGCCTCAAG CCAAAAGCCA GCAGCGAATG 
TTCTTTCTCT TTGACCACCA GCTCATCTAC TGTAAGAAGG ACCTGCTCCG CCGCGACGTG 
TTGTACTACA AGGGCCGGCT GGACATGGAC GGCCTGGAGG TGGTGGACCT GGAGGACGGG 
AAGGACAGAG ACCTCCATGT GAGCATCAAG AACGCCTTCC GGCTGCACCG TGGCGCCACA 
GGGGACAGCC ACCTGCTGTG CACCAGGAAG CCCGAGCAGA AGCAGCGCTG GCTCAAGGCC 
TTTGCCAGGG AGAGGGAGCA GGTGCAGCTG GACCAGGAGA CAGGCTTCTC CATCACTGAA 
CTGCAGAGGA AGCAGGCCAT GCTGAATGCC AGCAAGCAGC AGGTCACAGG GAAGCCCAAA 
GCTGTTGGCC GGCCCTGCTA CCTGACGCGC CAGAAGCACC CAGCCCTGCC CAGCAACCGG 
CCCCAGCAGC AGGTCCTGGT GCTGGCGGAG CCCAGGCGCA AGCCATCTAC CTTCTGGCAC 
AGCATCAGCC GGCTGGCACC CTTCCGCAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056135.2
CDS	520..2592
Translation	MPWEEPAGEKPSCSHSQKAFHMEPAQKPCFTTDMVTWALLCISAETVRGEAPSQPRGIPHRSPVSVDDLWLEKTQRKKLQKQAHVERRLHIGAVHKDGVKCWRKTIITSPESLNLPRRSHPLSQSAPTGLNHMGWPEHTPGTAMPDGALDTAVCADEVGSEEDLYDDLHSSSHHYSHPGGGGEQLAINELISDGSVVCAEALWDHVTMDDQELGFKAGDVIEVMDATNREWWWGRVADGEGWFPASFVRLRVNQDEPADDDAPLAGNSGAEDGGAEAQSSKDQMRTNVINEILSTERDYIKHLRDICEGYVRQCRKRADMFSEEQLRTIFGNIEDIYRCQKAFVKALEQRFNRERPHLSELGACFLEHQADFQIYSEYCNNHPNACVELSRLTKLSKYVYFFEACRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTHPQHRDFKDVEAALHAMKNVAQLINERKRRLENIDKIAQWQSSIEDWEGEDLLVRSSELIYSGELTRVTQPQAKSQQRMFFLFDHQLIYCKKDLLRRDVLYYKGRLDMDGLEVVDLEDGKDRDLHVSIKNAFRLHRGATGDSHLLCTRKPEQKQRWLKAFAREREQVQLDQETGFSITELQRKQAMLNASKQQVTGKPKAVGRPCYLTRQKHPALPSNRPQQQVLVLAEPRRKPSTFWHSISRLAPFRK

Target ORF information:

RefSeq Version	NM_015320.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015320.3

ORF Insert Sequence:

ATGCCCTGGG AAGAACCAGC AGGTGAGAAG CCCAGTTGCT CTCACAGTCA GAAGGCGTTC 
CACATGGAGC CTGCCCAGAA GCCCTGCTTC ACCACTGACA TGGTGACATG GGCCCTCCTC 
TGCATCTCTG CAGAGACTGT GCGTGGGGAG GCTCCTTCAC AGCCTAGGGG CATCCCTCAC 
CGCTCGCCCG TCAGTGTGGA TGACCTGTGG CTGGAGAAGA CACAGAGAAA GAAGTTGCAG 
AAGCAGGCCC ACGTCGAAAG GAGGCTGCAC ATAGGGGCAG TGCACAAAGA TGGAGTCAAG 
TGCTGGAGAA AGACGATCAT TACCTCTCCA GAGTCTTTGA ATCTCCCTAG AAGAAGCCAT 
CCACTCTCCC AGAGTGCTCC AACGGGACTG AACCACATGG GCTGGCCAGA GCACACACCA 
GGCACTGCCA TGCCTGATGG AGCTCTGGAC ACAGCTGTCT GCGCTGACGA AGTGGGGAGC 
GAGGAGGACC TGTATGATGA CCTGCACAGC TCCAGCCACC ACTACAGCCA CCCTGGAGGG 
GGTGGGGAGC AGCTGGCTAT CAATGAGCTC ATCAGCGATG GCAGTGTGGT CTGCGCTGAA 
GCACTCTGGG ACCATGTCAC CATGGACGAC CAGGAGCTGG GCTTCAAAGC TGGGGACGTC 
ATCGAAGTGA TGGATGCCAC CAACAGAGAG TGGTGGTGGG GCCGGGTCGC CGATGGCGAG 
GGCTGGTTTC CAGCCAGCTT CGTTCGGCTG AGGGTGAATC AGGACGAGCC CGCGGATGAC 
GACGCCCCTC TGGCCGGGAA CAGCGGAGCG GAGGACGGCG GGGCGGAGGC GCAGAGCAGC 
AAGGACCAGA TGCGGACCAA CGTCATCAAC GAGATCCTCA GCACTGAGCG GGACTACATC 
AAGCACCTGC GCGACATCTG CGAGGGCTAC GTCCGGCAGT GCCGCAAGCG CGCAGACATG 
TTCAGCGAGG AGCAGCTGCG TACCATCTTC GGGAACATCG AGGACATCTA CCGCTGCCAG 
AAGGCCTTCG TGAAGGCCCT GGAGCAGAGG TTCAACCGCG AGCGCCCACA CCTGAGCGAG 
CTGGGTGCCT GCTTCCTGGA GCATCAAGCC GACTTCCAGA TCTACTCGGA GTACTGCAAT 
AACCACCCCA ACGCCTGCGT GGAGCTCTCC CGGCTCACCA AGCTCAGCAA GTACGTGTAC 
TTCTTCGAGG CCTGCCGGCT GCTGCAGAAG ATGATTGACA TCTCCCTGGA TGGCTTCCTG 
CTGACTCCGG TGCAGAAGAT CTGCAAGTAC CCTCTGCAGC TGGCCGAGCT GCTCAAATAC 
ACGCACCCCC AGCACAGGGA CTTCAAGGAT GTTGAAGCCG CCTTGCATGC CATGAAGAAC 
GTGGCCCAGC TCATCAACGA GCGGAAGCGG AGACTTGAGA ACATCGACAA GATTGCTCAG 
TGGCAGAGCT CCATAGAGGA CTGGGAGGGA GAAGATCTCT TGGTCAGGAG CTCAGAACTC 
ATCTACTCGG GGGAGCTGAC TCGAGTTACA CAGCCTCAAG CCAAAAGCCA GCAGCGAATG 
TTCTTTCTCT TTGACCACCA GCTCATCTAC TGTAAGAAGG ACCTGCTCCG CCGCGACGTG 
TTGTACTACA AGGGCCGGCT GGACATGGAC GGCCTGGAGG TGGTGGACCT GGAGGACGGG 
AAGGACAGAG ACCTCCATGT GAGCATCAAG AACGCCTTCC GGCTGCACCG TGGCGCCACA 
GGGGACAGCC ACCTGCTGTG CACCAGGAAG CCCGAGCAGA AGCAGCGCTG GCTCAAGGCC 
TTTGCCAGGG AGAGGGAGCA GGTGCAGCTG GACCAGGAGA CAGGCTTCTC CATCACTGAA 
CTGCAGAGGA AGCAGGCCAT GCTGAATGCC AGCAAGCAGC AGGTCACAGG GAAGCCCAAA 
GCTGTTGGCC GGCCCTGCTA CCTGACGCGC CAGAAGCACC CAGCCCTGCC CAGCAACCGG 
CCCCAGCAGC AGGTCCTGGT GCTGGCGGAG CCCAGGCGCA AGCCATCTAC CTTCTGGCAC 
AGCATCAGCC GGCTGGCACC CTTCCGCAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF4 ( NM_015320.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_056135.2 Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu08729
Clone ID Related Accession (Same CDS sequence)	NM_015320.3 , NM_015320.4
Accession Version	NM_015320.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-05
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 4 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC133785.2, AC112786.4, AF249745.1, AB042199.1 and AL137289.1. On or before Dec 4, 2018 this sequence version replaced XM_005263685.3, NM_015320.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF249745.1, SRR1803613.232068.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2145240, SAMEA2145313 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCCTGGG AAGAACCAGC AGGTGAGAAG CCCAGTTGCT CTCACAGTCA GAAGGCGTTC 
CACATGGAGC CTGCCCAGAA GCCCTGCTTC ACCACTGACA TGGTGACATG GGCCCTCCTC 
TGCATCTCTG CAGAGACTGT GCGTGGGGAG GCTCCTTCAC AGCCTAGGGG CATCCCTCAC 
CGCTCGCCCG TCAGTGTGGA TGACCTGTGG CTGGAGAAGA CACAGAGAAA GAAGTTGCAG 
AAGCAGGCCC ACGTCGAAAG GAGGCTGCAC ATAGGGGCAG TGCACAAAGA TGGAGTCAAG 
TGCTGGAGAA AGACGATCAT TACCTCTCCA GAGTCTTTGA ATCTCCCTAG AAGAAGCCAT 
CCACTCTCCC AGAGTGCTCC AACGGGACTG AACCACATGG GCTGGCCAGA GCACACACCA 
GGCACTGCCA TGCCTGATGG AGCTCTGGAC ACAGCTGTCT GCGCTGACGA AGTGGGGAGC 
GAGGAGGACC TGTATGATGA CCTGCACAGC TCCAGCCACC ACTACAGCCA CCCTGGAGGG 
GGTGGGGAGC AGCTGGCTAT CAATGAGCTC ATCAGCGATG GCAGTGTGGT CTGCGCTGAA 
GCACTCTGGG ACCATGTCAC CATGGACGAC CAGGAGCTGG GCTTCAAAGC TGGGGACGTC 
ATCGAAGTGA TGGATGCCAC CAACAGAGAG TGGTGGTGGG GCCGGGTCGC CGATGGCGAG 
GGCTGGTTTC CAGCCAGCTT CGTTCGGCTG AGGGTGAATC AGGACGAGCC CGCGGATGAC 
GACGCCCCTC TGGCCGGGAA CAGCGGAGCG GAGGACGGCG GGGCGGAGGC GCAGAGCAGC 
AAGGACCAGA TGCGGACCAA CGTCATCAAC GAGATCCTCA GCACTGAGCG GGACTACATC 
AAGCACCTGC GCGACATCTG CGAGGGCTAC GTCCGGCAGT GCCGCAAGCG CGCAGACATG 
TTCAGCGAGG AGCAGCTGCG TACCATCTTC GGGAACATCG AGGACATCTA CCGCTGCCAG 
AAGGCCTTCG TGAAGGCCCT GGAGCAGAGG TTCAACCGCG AGCGCCCACA CCTGAGCGAG 
CTGGGTGCCT GCTTCCTGGA GCATCAAGCC GACTTCCAGA TCTACTCGGA GTACTGCAAT 
AACCACCCCA ACGCCTGCGT GGAGCTCTCC CGGCTCACCA AGCTCAGCAA GTACGTGTAC 
TTCTTCGAGG CCTGCCGGCT GCTGCAGAAG ATGATTGACA TCTCCCTGGA TGGCTTCCTG 
CTGACTCCGG TGCAGAAGAT CTGCAAGTAC CCTCTGCAGC TGGCCGAGCT GCTCAAATAC 
ACGCACCCCC AGCACAGGGA CTTCAAGGAT GTTGAAGCCG CCTTGCATGC CATGAAGAAC 
GTGGCCCAGC TCATCAACGA GCGGAAGCGG AGACTTGAGA ACATCGACAA GATTGCTCAG 
TGGCAGAGCT CCATAGAGGA CTGGGAGGGA GAAGATCTCT TGGTCAGGAG CTCAGAACTC 
ATCTACTCGG GGGAGCTGAC TCGAGTTACA CAGCCTCAAG CCAAAAGCCA GCAGCGAATG 
TTCTTTCTCT TTGACCACCA GCTCATCTAC TGTAAGAAGG ACCTGCTCCG CCGCGACGTG 
TTGTACTACA AGGGCCGGCT GGACATGGAC GGCCTGGAGG TGGTGGACCT GGAGGACGGG 
AAGGACAGAG ACCTCCATGT GAGCATCAAG AACGCCTTCC GGCTGCACCG TGGCGCCACA 
GGGGACAGCC ACCTGCTGTG CACCAGGAAG CCCGAGCAGA AGCAGCGCTG GCTCAAGGCC 
TTTGCCAGGG AGAGGGAGCA GGTGCAGCTG GACCAGGAGA CAGGCTTCTC CATCACTGAA 
CTGCAGAGGA AGCAGGCCAT GCTGAATGCC AGCAAGCAGC AGGTCACAGG GAAGCCCAAA 
GCTGTTGGCC GGCCCTGCTA CCTGACGCGC CAGAAGCACC CAGCCCTGCC CAGCAACCGG 
CCCCAGCAGC AGGTCCTGGT GCTGGCGGAG CCCAGGCGCA AGCCATCTAC CTTCTGGCAC 
AGCATCAGCC GGCTGGCACC CTTCCGCAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056135.2
CDS	3264..5336
Translation	MPWEEPAGEKPSCSHSQKAFHMEPAQKPCFTTDMVTWALLCISAETVRGEAPSQPRGIPHRSPVSVDDLWLEKTQRKKLQKQAHVERRLHIGAVHKDGVKCWRKTIITSPESLNLPRRSHPLSQSAPTGLNHMGWPEHTPGTAMPDGALDTAVCADEVGSEEDLYDDLHSSSHHYSHPGGGGEQLAINELISDGSVVCAEALWDHVTMDDQELGFKAGDVIEVMDATNREWWWGRVADGEGWFPASFVRLRVNQDEPADDDAPLAGNSGAEDGGAEAQSSKDQMRTNVINEILSTERDYIKHLRDICEGYVRQCRKRADMFSEEQLRTIFGNIEDIYRCQKAFVKALEQRFNRERPHLSELGACFLEHQADFQIYSEYCNNHPNACVELSRLTKLSKYVYFFEACRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTHPQHRDFKDVEAALHAMKNVAQLINERKRRLENIDKIAQWQSSIEDWEGEDLLVRSSELIYSGELTRVTQPQAKSQQRMFFLFDHQLIYCKKDLLRRDVLYYKGRLDMDGLEVVDLEDGKDRDLHVSIKNAFRLHRGATGDSHLLCTRKPEQKQRWLKAFAREREQVQLDQETGFSITELQRKQAMLNASKQQVTGKPKAVGRPCYLTRQKHPALPSNRPQQQVLVLAEPRRKPSTFWHSISRLAPFRK

Target ORF information:

RefSeq Version	NM_015320.4
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015320.4

ORF Insert Sequence:

ATGCCCTGGG AAGAACCAGC AGGTGAGAAG CCCAGTTGCT CTCACAGTCA GAAGGCGTTC 
CACATGGAGC CTGCCCAGAA GCCCTGCTTC ACCACTGACA TGGTGACATG GGCCCTCCTC 
TGCATCTCTG CAGAGACTGT GCGTGGGGAG GCTCCTTCAC AGCCTAGGGG CATCCCTCAC 
CGCTCGCCCG TCAGTGTGGA TGACCTGTGG CTGGAGAAGA CACAGAGAAA GAAGTTGCAG 
AAGCAGGCCC ACGTCGAAAG GAGGCTGCAC ATAGGGGCAG TGCACAAAGA TGGAGTCAAG 
TGCTGGAGAA AGACGATCAT TACCTCTCCA GAGTCTTTGA ATCTCCCTAG AAGAAGCCAT 
CCACTCTCCC AGAGTGCTCC AACGGGACTG AACCACATGG GCTGGCCAGA GCACACACCA 
GGCACTGCCA TGCCTGATGG AGCTCTGGAC ACAGCTGTCT GCGCTGACGA AGTGGGGAGC 
GAGGAGGACC TGTATGATGA CCTGCACAGC TCCAGCCACC ACTACAGCCA CCCTGGAGGG 
GGTGGGGAGC AGCTGGCTAT CAATGAGCTC ATCAGCGATG GCAGTGTGGT CTGCGCTGAA 
GCACTCTGGG ACCATGTCAC CATGGACGAC CAGGAGCTGG GCTTCAAAGC TGGGGACGTC 
ATCGAAGTGA TGGATGCCAC CAACAGAGAG TGGTGGTGGG GCCGGGTCGC CGATGGCGAG 
GGCTGGTTTC CAGCCAGCTT CGTTCGGCTG AGGGTGAATC AGGACGAGCC CGCGGATGAC 
GACGCCCCTC TGGCCGGGAA CAGCGGAGCG GAGGACGGCG GGGCGGAGGC GCAGAGCAGC 
AAGGACCAGA TGCGGACCAA CGTCATCAAC GAGATCCTCA GCACTGAGCG GGACTACATC 
AAGCACCTGC GCGACATCTG CGAGGGCTAC GTCCGGCAGT GCCGCAAGCG CGCAGACATG 
TTCAGCGAGG AGCAGCTGCG TACCATCTTC GGGAACATCG AGGACATCTA CCGCTGCCAG 
AAGGCCTTCG TGAAGGCCCT GGAGCAGAGG TTCAACCGCG AGCGCCCACA CCTGAGCGAG 
CTGGGTGCCT GCTTCCTGGA GCATCAAGCC GACTTCCAGA TCTACTCGGA GTACTGCAAT 
AACCACCCCA ACGCCTGCGT GGAGCTCTCC CGGCTCACCA AGCTCAGCAA GTACGTGTAC 
TTCTTCGAGG CCTGCCGGCT GCTGCAGAAG ATGATTGACA TCTCCCTGGA TGGCTTCCTG 
CTGACTCCGG TGCAGAAGAT CTGCAAGTAC CCTCTGCAGC TGGCCGAGCT GCTCAAATAC 
ACGCACCCCC AGCACAGGGA CTTCAAGGAT GTTGAAGCCG CCTTGCATGC CATGAAGAAC 
GTGGCCCAGC TCATCAACGA GCGGAAGCGG AGACTTGAGA ACATCGACAA GATTGCTCAG 
TGGCAGAGCT CCATAGAGGA CTGGGAGGGA GAAGATCTCT TGGTCAGGAG CTCAGAACTC 
ATCTACTCGG GGGAGCTGAC TCGAGTTACA CAGCCTCAAG CCAAAAGCCA GCAGCGAATG 
TTCTTTCTCT TTGACCACCA GCTCATCTAC TGTAAGAAGG ACCTGCTCCG CCGCGACGTG 
TTGTACTACA AGGGCCGGCT GGACATGGAC GGCCTGGAGG TGGTGGACCT GGAGGACGGG 
AAGGACAGAG ACCTCCATGT GAGCATCAAG AACGCCTTCC GGCTGCACCG TGGCGCCACA 
GGGGACAGCC ACCTGCTGTG CACCAGGAAG CCCGAGCAGA AGCAGCGCTG GCTCAAGGCC 
TTTGCCAGGG AGAGGGAGCA GGTGCAGCTG GACCAGGAGA CAGGCTTCTC CATCACTGAA 
CTGCAGAGGA AGCAGGCCAT GCTGAATGCC AGCAAGCAGC AGGTCACAGG GAAGCCCAAA 
GCTGTTGGCC GGCCCTGCTA CCTGACGCGC CAGAAGCACC CAGCCCTGCC CAGCAACCGG 
CCCCAGCAGC AGGTCCTGGT GCTGGCGGAG CCCAGGCGCA AGCCATCTAC CTTCTGGCAC 
AGCATCAGCC GGCTGGCACC CTTCCGCAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF4 ( NM_032995.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_127462.1 Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant 2, mRNA.

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CloneID	OHu09786
Clone ID Related Accession (Same CDS sequence)	NM_032995.2
Accession Version	NM_032995.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 4 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF249745.1, AB029035.1 and AL137289.1. On Jun 25, 2013 this sequence version replaced NM_032995.1. Transcript Variant: This variant (2) has an additional segment in the 3' region, compared to variant 1. This causes the reading frame shift, and the resulting isoform (b) is shorter and has a distinct C-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB029035.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2144120 [ECO:0000348] ##Evidence-Data-END##

ATGCCCTGGG AAGAACCAGC AGGTGAGAAG CCCAGTTGCT CTCACAGTCA GAAGGCGTTC 
CACATGGAGC CTGCCCAGAA GCCCTGCTTC ACCACTGACA TGGTGACATG GGCCCTCCTC 
TGCATCTCTG CAGAGACTGT GCGTGGGGAG GCTCCTTCAC AGCCTAGGGG CATCCCTCAC 
CGCTCGCCCG TCAGTGTGGA TGACCTGTGG CTGGAGAAGA CACAGAGAAA GAAGTTGCAG 
AAGCAGGCCC ACGTCGAAAG GAGGCTGCAC ATAGGGGCAG TGCACAAAGA TGGAGTCAAG 
TGCTGGAGAA AGACGATCAT TACCTCTCCA GAGTCTTTGA ATCTCCCTAG AAGAAGCCAT 
CCACTCTCCC AGAGTGCTCC AACGGGACTG AACCACATGG GCTGGCCAGA GCACACACCA 
GGCACTGCCA TGCCTGATGG AGCTCTGGAC ACAGCTGTCT GCGCTGACGA AGTGGGGAGC 
GAGGAGGACC TGTATGATGA CCTGCACAGC TCCAGCCACC ACTACAGCCA CCCTGGAGGG 
GGTGGGGAGC AGCTGGCTAT CAATGAGCTC ATCAGCGATG GCAGTGTGGT CTGCGCTGAA 
GCACTCTGGG ACCATGTCAC CATGGACGAC CAGGAGCTGG GCTTCAAAGC TGGGGACGTC 
ATCGAAGTGA TGGATGCCAC CAACAGAGAG TGGTGGTGGG GCCGGGTCGC CGATGGCGAG 
GGCTGGTTTC CAGCCAGCTT CGTTCGGCTG AGGGTGAATC AGGACGAGCC CGCGGATGAC 
GACGCCCCTC TGGCCGGGAA CAGCGGAGCG GAGGACGGCG GGGCGGAGGC GCAGAGCAGC 
AAGGACCAGA TGCGGACCAA CGTCATCAAC GAGATCCTCA GCACTGAGCG GGACTACATC 
AAGCACCTGC GCGACATCTG CGAGGGCTAC GTCCGGCAGT GCCGCAAGCG CGCAGACATG 
TTCAGCGAGG AGCAGCTGCG TACCATCTTC GGGAACATCG AGGACATCTA CCGCTGCCAG 
AAGGCCTTCG TGAAGGCCCT GGAGCAGAGG TTCAACCGCG AGCGCCCACA CCTGAGCGAG 
CTGGGTGCCT GCTTCCTGGA GCATCAAGCC GACTTCCAGA TCTACTCGGA GTACTGCAAT 
AACCACCCCA ACGCCTGCGT GGAGCTCTCC CGGCTCACCA AGCTCAGCAA GTACGTGTAC 
TTCTTCGAGG CCTGCCGGCT GCTGCAGAAG ATGATTGACA TCTCCCTGGA TGGCTTCCTG 
CTGACTCCGG TGCAGAAGAT CTGCAAGTAC CCTCTGCAGC TGGCCGAGCT GCTCAAATAC 
ACGCACCCCC AGCACAGGGA CTTCAAGGAT GTTGAAGCCG CCTTGCATGC CATGAAGAAC 
GTGGCCCAGC TCATCAACGA GCGGAAGCGG AGACTTGAGA ACATCGACAA GATTGCTCAG 
TGGCAGAGCT CCATAGAGGA CTGGGAGGGA GAAGATCTCT TGGTCAGGAG CTCAGAACTC 
ATCTACTCGG GGGAGCTGAC TCGAGTTACA CAGCCTCAAG CCAAAAGCCA GCAGCGAATG 
TTCTTTCTCT TTGACCACCA GCTCATCTAC TGTAAGAAGG ACCTGCTCCG CCGCGACGTG 
TTGTACTACA AGGGCCGGCT GGACATGGAC GGCCTGGAGG TGGTGGACCT GGAGGACGGG 
AAGGACAGAG ACCTCCATGT GAGCATCAAG AACGCCTTCC GGCTGCACCG TGGCGCCACA 
GGGGACAGCC ACCTGCTGTG CACCAGGAAG CCCGAGCAGA AGCAGCGCTG GCTCAAGGCC 
TTTGCCAGGG AGAGGGAGCA GGTGCAGCTG GACCAGGAGA CAGGCTTCTC CATCACTGAA 
CTGCAGAGGA AGCAGGCCAT GCTGAATGCC AGCAAGCAGC AGGTCACAGG GAAGCCCAAA 
GGTAGGCGGA CAGCAGCCCC ACCTCCTCGG CTGCCCGGTC CTTACCCTGC TGACATCATT 
CCCTTCTCTG AACCACAAAG CCAAGCCAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_127462.1
CDS	109..2121
Translation	MPWEEPAGEKPSCSHSQKAFHMEPAQKPCFTTDMVTWALLCISAETVRGEAPSQPRGIPHRSPVSVDDLWLEKTQRKKLQKQAHVERRLHIGAVHKDGVKCWRKTIITSPESLNLPRRSHPLSQSAPTGLNHMGWPEHTPGTAMPDGALDTAVCADEVGSEEDLYDDLHSSSHHYSHPGGGGEQLAINELISDGSVVCAEALWDHVTMDDQELGFKAGDVIEVMDATNREWWWGRVADGEGWFPASFVRLRVNQDEPADDDAPLAGNSGAEDGGAEAQSSKDQMRTNVINEILSTERDYIKHLRDICEGYVRQCRKRADMFSEEQLRTIFGNIEDIYRCQKAFVKALEQRFNRERPHLSELGACFLEHQADFQIYSEYCNNHPNACVELSRLTKLSKYVYFFEACRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTHPQHRDFKDVEAALHAMKNVAQLINERKRRLENIDKIAQWQSSIEDWEGEDLLVRSSELIYSGELTRVTQPQAKSQQRMFFLFDHQLIYCKKDLLRRDVLYYKGRLDMDGLEVVDLEDGKDRDLHVSIKNAFRLHRGATGDSHLLCTRKPEQKQRWLKAFAREREQVQLDQETGFSITELQRKQAMLNASKQQVTGKPKGRRTAAPPPRLPGPYPADIIPFSEPQSQAS

Target ORF information:

RefSeq Version	NM_032995.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032995.2

ORF Insert Sequence:

ATGCCCTGGG AAGAACCAGC AGGTGAGAAG CCCAGTTGCT CTCACAGTCA GAAGGCGTTC 
CACATGGAGC CTGCCCAGAA GCCCTGCTTC ACCACTGACA TGGTGACATG GGCCCTCCTC 
TGCATCTCTG CAGAGACTGT GCGTGGGGAG GCTCCTTCAC AGCCTAGGGG CATCCCTCAC 
CGCTCGCCCG TCAGTGTGGA TGACCTGTGG CTGGAGAAGA CACAGAGAAA GAAGTTGCAG 
AAGCAGGCCC ACGTCGAAAG GAGGCTGCAC ATAGGGGCAG TGCACAAAGA TGGAGTCAAG 
TGCTGGAGAA AGACGATCAT TACCTCTCCA GAGTCTTTGA ATCTCCCTAG AAGAAGCCAT 
CCACTCTCCC AGAGTGCTCC AACGGGACTG AACCACATGG GCTGGCCAGA GCACACACCA 
GGCACTGCCA TGCCTGATGG AGCTCTGGAC ACAGCTGTCT GCGCTGACGA AGTGGGGAGC 
GAGGAGGACC TGTATGATGA CCTGCACAGC TCCAGCCACC ACTACAGCCA CCCTGGAGGG 
GGTGGGGAGC AGCTGGCTAT CAATGAGCTC ATCAGCGATG GCAGTGTGGT CTGCGCTGAA 
GCACTCTGGG ACCATGTCAC CATGGACGAC CAGGAGCTGG GCTTCAAAGC TGGGGACGTC 
ATCGAAGTGA TGGATGCCAC CAACAGAGAG TGGTGGTGGG GCCGGGTCGC CGATGGCGAG 
GGCTGGTTTC CAGCCAGCTT CGTTCGGCTG AGGGTGAATC AGGACGAGCC CGCGGATGAC 
GACGCCCCTC TGGCCGGGAA CAGCGGAGCG GAGGACGGCG GGGCGGAGGC GCAGAGCAGC 
AAGGACCAGA TGCGGACCAA CGTCATCAAC GAGATCCTCA GCACTGAGCG GGACTACATC 
AAGCACCTGC GCGACATCTG CGAGGGCTAC GTCCGGCAGT GCCGCAAGCG CGCAGACATG 
TTCAGCGAGG AGCAGCTGCG TACCATCTTC GGGAACATCG AGGACATCTA CCGCTGCCAG 
AAGGCCTTCG TGAAGGCCCT GGAGCAGAGG TTCAACCGCG AGCGCCCACA CCTGAGCGAG 
CTGGGTGCCT GCTTCCTGGA GCATCAAGCC GACTTCCAGA TCTACTCGGA GTACTGCAAT 
AACCACCCCA ACGCCTGCGT GGAGCTCTCC CGGCTCACCA AGCTCAGCAA GTACGTGTAC 
TTCTTCGAGG CCTGCCGGCT GCTGCAGAAG ATGATTGACA TCTCCCTGGA TGGCTTCCTG 
CTGACTCCGG TGCAGAAGAT CTGCAAGTAC CCTCTGCAGC TGGCCGAGCT GCTCAAATAC 
ACGCACCCCC AGCACAGGGA CTTCAAGGAT GTTGAAGCCG CCTTGCATGC CATGAAGAAC 
GTGGCCCAGC TCATCAACGA GCGGAAGCGG AGACTTGAGA ACATCGACAA GATTGCTCAG 
TGGCAGAGCT CCATAGAGGA CTGGGAGGGA GAAGATCTCT TGGTCAGGAG CTCAGAACTC 
ATCTACTCGG GGGAGCTGAC TCGAGTTACA CAGCCTCAAG CCAAAAGCCA GCAGCGAATG 
TTCTTTCTCT TTGACCACCA GCTCATCTAC TGTAAGAAGG ACCTGCTCCG CCGCGACGTG 
TTGTACTACA AGGGCCGGCT GGACATGGAC GGCCTGGAGG TGGTGGACCT GGAGGACGGG 
AAGGACAGAG ACCTCCATGT GAGCATCAAG AACGCCTTCC GGCTGCACCG TGGCGCCACA 
GGGGACAGCC ACCTGCTGTG CACCAGGAAG CCCGAGCAGA AGCAGCGCTG GCTCAAGGCC 
TTTGCCAGGG AGAGGGAGCA GGTGCAGCTG GACCAGGAGA CAGGCTTCTC CATCACTGAA 
CTGCAGAGGA AGCAGGCCAT GCTGAATGCC AGCAAGCAGC AGGTCACAGG GAAGCCCAAA 
GGTAGGCGGA CAGCAGCCCC ACCTCCTCGG CTGCCCGGTC CTTACCCTGC TGACATCATT 
CCCTTCTCTG AACCACAAAG CCAAGCCAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF4 ( XM_005263688.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005263745.1 Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X8, mRNA.

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CloneID	OHu32422
Clone ID Related Accession (Same CDS sequence)	XM_005263688.3 , XM_005263689.4 , XM_011511277.2 , XM_011511277.1 , XM_005263689.5 , XM_024452938.1 , XM_011511276.2 , XM_005263688.2 , XM_011511276.3
Accession Version	XM_005263688.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005263688.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGACC AGGAGCTGGG CTTCAAAGCT GGGGACGTCA TCGAAGTGAT GGATGCCACC 
AACAGAGAGT GGTGGTGGGG CCGGGTCGCC GATGGCGAGG GCTGGTTTCC AGCCAGCTTC 
GTTCGGCTGA GGGTGAATCA GGACGAGCCC GCGGATGACG ACGCCCCTCT GGCCGGGAAC 
AGCGGAGCGG AGGACGGCGG GGCGGAGGCG CAGAGCAGCA AGGACCAGAT GCGGACCAAC 
GTCATCAACG AGATCCTCAG CACTGAGCGG GACTACATCA AGCACCTGCG CGACATCTGC 
GAGGGCTACG TCCGGCAGTG CCGCAAGCGC GCAGACATGT TCAGCGAGGA GCAGCTGCGT 
ACCATCTTCG GGAACATCGA GGACATCTAC CGCTGCCAGA AGGCCTTCGT GAAGGCCCTG 
GAGCAGAGGT TCAACCGCGA GCGCCCACAC CTGAGCGAGC TGGGTGCCTG CTTCCTGGAG 
CATCAAGCCG ACTTCCAGAT CTACTCGGAG TACTGCAATA ACCACCCCAA CGCCTGCGTG 
GAGCTCTCCC GGCTCACCAA GCTCAGCAAG TACGTGTACT TCTTCGAGGC CTGCCGGCTG 
CTGCAGAAGA TGATTGACAT CTCCCTGGAT GGCTTCCTGC TGACTCCGGT GCAGAAGATC 
TGCAAGTACC CTCTGCAGCT GGCCGAGCTG CTCAAATACA CGCACCCCCA GCACAGGGAC 
TTCAAGGATG TTGAAGCCGC CTTGCATGCC ATGAAGAACG TGGCCCAGCT CATCAACGAG 
CGGAAGCGGA GACTTGAGAA CATCGACAAG ATTGCTCAGT GGCAGAGCTC CATAGAGGAC 
TGGGAGGGAG AAGATCTCTT GGTCAGGAGC TCAGAACTCA TCTACTCGGG GGAGCTGACT 
CGAGTTACAC AGCCTCAAGC CAAAAGCCAG CAGCGAATGT TCTTTCTCTT TGACCACCAG 
CTCATCTACT GTAAGAAGGA CCTGCTCCGC CGCGACGTGT TGTACTACAA GGGCCGGCTG 
GACATGGACG GCCTGGAGGT GGTGGACCTG GAGGACGGGA AGGACAGAGA CCTCCATGTG 
AGCATCAAGA ACGCCTTCCG GCTGCACCGT GGCGCCACAG GGGACAGCCA CCTGCTGTGC 
ACCAGGAAGC CCGAGCAGAA GCAGCGCTGG CTCAAGGCCT TTGCCAGGGA GAGGGAGCAG 
GTGCAGCTGG ACCAGGAGAC AGGCTTCTCC ATCACTGAAC TGCAGAGGAA GCAGGCCATG 
CTGAATGCCA GCAAGCAGCA GGTCACAGGG AAGCCCAAAG CTGTTGGCCG GCCCTGCTAC 
CTGACGCGCC AGAAGCACCC AGCCCTGCCC AGCAACCGGC CCCAGCAGCA GGTCCTGGTG 
CTGGCGGAGC CCAGGCGCAA GCCATCTACC TTCTGGCACA GCATCAGCCG GCTGGCACCC 
TTCCGCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005263745.1
CDS	362..1813
Translation	MDDQELGFKAGDVIEVMDATNREWWWGRVADGEGWFPASFVRLRVNQDEPADDDAPLAGNSGAEDGGAEAQSSKDQMRTNVINEILSTERDYIKHLRDICEGYVRQCRKRADMFSEEQLRTIFGNIEDIYRCQKAFVKALEQRFNRERPHLSELGACFLEHQADFQIYSEYCNNHPNACVELSRLTKLSKYVYFFEACRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTHPQHRDFKDVEAALHAMKNVAQLINERKRRLENIDKIAQWQSSIEDWEGEDLLVRSSELIYSGELTRVTQPQAKSQQRMFFLFDHQLIYCKKDLLRRDVLYYKGRLDMDGLEVVDLEDGKDRDLHVSIKNAFRLHRGATGDSHLLCTRKPEQKQRWLKAFAREREQVQLDQETGFSITELQRKQAMLNASKQQVTGKPKAVGRPCYLTRQKHPALPSNRPQQQVLVLAEPRRKPSTFWHSISRLAPFRK

Target ORF information:

RefSeq Version	XM_005263688.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005263688.3

ORF Insert Sequence:

ATGGACGACC AGGAGCTGGG CTTCAAAGCT GGGGACGTCA TCGAAGTGAT GGATGCCACC 
AACAGAGAGT GGTGGTGGGG CCGGGTCGCC GATGGCGAGG GCTGGTTTCC AGCCAGCTTC 
GTTCGGCTGA GGGTGAATCA GGACGAGCCC GCGGATGACG ACGCCCCTCT GGCCGGGAAC 
AGCGGAGCGG AGGACGGCGG GGCGGAGGCG CAGAGCAGCA AGGACCAGAT GCGGACCAAC 
GTCATCAACG AGATCCTCAG CACTGAGCGG GACTACATCA AGCACCTGCG CGACATCTGC 
GAGGGCTACG TCCGGCAGTG CCGCAAGCGC GCAGACATGT TCAGCGAGGA GCAGCTGCGT 
ACCATCTTCG GGAACATCGA GGACATCTAC CGCTGCCAGA AGGCCTTCGT GAAGGCCCTG 
GAGCAGAGGT TCAACCGCGA GCGCCCACAC CTGAGCGAGC TGGGTGCCTG CTTCCTGGAG 
CATCAAGCCG ACTTCCAGAT CTACTCGGAG TACTGCAATA ACCACCCCAA CGCCTGCGTG 
GAGCTCTCCC GGCTCACCAA GCTCAGCAAG TACGTGTACT TCTTCGAGGC CTGCCGGCTG 
CTGCAGAAGA TGATTGACAT CTCCCTGGAT GGCTTCCTGC TGACTCCGGT GCAGAAGATC 
TGCAAGTACC CTCTGCAGCT GGCCGAGCTG CTCAAATACA CGCACCCCCA GCACAGGGAC 
TTCAAGGATG TTGAAGCCGC CTTGCATGCC ATGAAGAACG TGGCCCAGCT CATCAACGAG 
CGGAAGCGGA GACTTGAGAA CATCGACAAG ATTGCTCAGT GGCAGAGCTC CATAGAGGAC 
TGGGAGGGAG AAGATCTCTT GGTCAGGAGC TCAGAACTCA TCTACTCGGG GGAGCTGACT 
CGAGTTACAC AGCCTCAAGC CAAAAGCCAG CAGCGAATGT TCTTTCTCTT TGACCACCAG 
CTCATCTACT GTAAGAAGGA CCTGCTCCGC CGCGACGTGT TGTACTACAA GGGCCGGCTG 
GACATGGACG GCCTGGAGGT GGTGGACCTG GAGGACGGGA AGGACAGAGA CCTCCATGTG 
AGCATCAAGA ACGCCTTCCG GCTGCACCGT GGCGCCACAG GGGACAGCCA CCTGCTGTGC 
ACCAGGAAGC CCGAGCAGAA GCAGCGCTGG CTCAAGGCCT TTGCCAGGGA GAGGGAGCAG 
GTGCAGCTGG ACCAGGAGAC AGGCTTCTCC ATCACTGAAC TGCAGAGGAA GCAGGCCATG 
CTGAATGCCA GCAAGCAGCA GGTCACAGGG AAGCCCAAAG CTGTTGGCCG GCCCTGCTAC 
CTGACGCGCC AGAAGCACCC AGCCCTGCCC AGCAACCGGC CCCAGCAGCA GGTCCTGGTG 
CTGGCGGAGC CCAGGCGCAA GCCATCTACC TTCTGGCACA GCATCAGCCG GCTGGCACCC 
TTCCGCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF4 ( XM_005263689.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005263746.1 Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu32422
Clone ID Related Accession (Same CDS sequence)	XM_005263688.3 , XM_005263689.4 , XM_011511277.2 , XM_011511277.1 , XM_005263689.5 , XM_024452938.1 , XM_011511276.2 , XM_005263688.2 , XM_011511276.3
Accession Version	XM_005263689.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005263689.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGACC AGGAGCTGGG CTTCAAAGCT GGGGACGTCA TCGAAGTGAT GGATGCCACC 
AACAGAGAGT GGTGGTGGGG CCGGGTCGCC GATGGCGAGG GCTGGTTTCC AGCCAGCTTC 
GTTCGGCTGA GGGTGAATCA GGACGAGCCC GCGGATGACG ACGCCCCTCT GGCCGGGAAC 
AGCGGAGCGG AGGACGGCGG GGCGGAGGCG CAGAGCAGCA AGGACCAGAT GCGGACCAAC 
GTCATCAACG AGATCCTCAG CACTGAGCGG GACTACATCA AGCACCTGCG CGACATCTGC 
GAGGGCTACG TCCGGCAGTG CCGCAAGCGC GCAGACATGT TCAGCGAGGA GCAGCTGCGT 
ACCATCTTCG GGAACATCGA GGACATCTAC CGCTGCCAGA AGGCCTTCGT GAAGGCCCTG 
GAGCAGAGGT TCAACCGCGA GCGCCCACAC CTGAGCGAGC TGGGTGCCTG CTTCCTGGAG 
CATCAAGCCG ACTTCCAGAT CTACTCGGAG TACTGCAATA ACCACCCCAA CGCCTGCGTG 
GAGCTCTCCC GGCTCACCAA GCTCAGCAAG TACGTGTACT TCTTCGAGGC CTGCCGGCTG 
CTGCAGAAGA TGATTGACAT CTCCCTGGAT GGCTTCCTGC TGACTCCGGT GCAGAAGATC 
TGCAAGTACC CTCTGCAGCT GGCCGAGCTG CTCAAATACA CGCACCCCCA GCACAGGGAC 
TTCAAGGATG TTGAAGCCGC CTTGCATGCC ATGAAGAACG TGGCCCAGCT CATCAACGAG 
CGGAAGCGGA GACTTGAGAA CATCGACAAG ATTGCTCAGT GGCAGAGCTC CATAGAGGAC 
TGGGAGGGAG AAGATCTCTT GGTCAGGAGC TCAGAACTCA TCTACTCGGG GGAGCTGACT 
CGAGTTACAC AGCCTCAAGC CAAAAGCCAG CAGCGAATGT TCTTTCTCTT TGACCACCAG 
CTCATCTACT GTAAGAAGGA CCTGCTCCGC CGCGACGTGT TGTACTACAA GGGCCGGCTG 
GACATGGACG GCCTGGAGGT GGTGGACCTG GAGGACGGGA AGGACAGAGA CCTCCATGTG 
AGCATCAAGA ACGCCTTCCG GCTGCACCGT GGCGCCACAG GGGACAGCCA CCTGCTGTGC 
ACCAGGAAGC CCGAGCAGAA GCAGCGCTGG CTCAAGGCCT TTGCCAGGGA GAGGGAGCAG 
GTGCAGCTGG ACCAGGAGAC AGGCTTCTCC ATCACTGAAC TGCAGAGGAA GCAGGCCATG 
CTGAATGCCA GCAAGCAGCA GGTCACAGGG AAGCCCAAAG CTGTTGGCCG GCCCTGCTAC 
CTGACGCGCC AGAAGCACCC AGCCCTGCCC AGCAACCGGC CCCAGCAGCA GGTCCTGGTG 
CTGGCGGAGC CCAGGCGCAA GCCATCTACC TTCTGGCACA GCATCAGCCG GCTGGCACCC 
TTCCGCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005263746.1
CDS	277..1728
Translation	MDDQELGFKAGDVIEVMDATNREWWWGRVADGEGWFPASFVRLRVNQDEPADDDAPLAGNSGAEDGGAEAQSSKDQMRTNVINEILSTERDYIKHLRDICEGYVRQCRKRADMFSEEQLRTIFGNIEDIYRCQKAFVKALEQRFNRERPHLSELGACFLEHQADFQIYSEYCNNHPNACVELSRLTKLSKYVYFFEACRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTHPQHRDFKDVEAALHAMKNVAQLINERKRRLENIDKIAQWQSSIEDWEGEDLLVRSSELIYSGELTRVTQPQAKSQQRMFFLFDHQLIYCKKDLLRRDVLYYKGRLDMDGLEVVDLEDGKDRDLHVSIKNAFRLHRGATGDSHLLCTRKPEQKQRWLKAFAREREQVQLDQETGFSITELQRKQAMLNASKQQVTGKPKAVGRPCYLTRQKHPALPSNRPQQQVLVLAEPRRKPSTFWHSISRLAPFRK

Target ORF information:

RefSeq Version	XM_005263689.4
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005263689.4

ORF Insert Sequence:

ATGGACGACC AGGAGCTGGG CTTCAAAGCT GGGGACGTCA TCGAAGTGAT GGATGCCACC 
AACAGAGAGT GGTGGTGGGG CCGGGTCGCC GATGGCGAGG GCTGGTTTCC AGCCAGCTTC 
GTTCGGCTGA GGGTGAATCA GGACGAGCCC GCGGATGACG ACGCCCCTCT GGCCGGGAAC 
AGCGGAGCGG AGGACGGCGG GGCGGAGGCG CAGAGCAGCA AGGACCAGAT GCGGACCAAC 
GTCATCAACG AGATCCTCAG CACTGAGCGG GACTACATCA AGCACCTGCG CGACATCTGC 
GAGGGCTACG TCCGGCAGTG CCGCAAGCGC GCAGACATGT TCAGCGAGGA GCAGCTGCGT 
ACCATCTTCG GGAACATCGA GGACATCTAC CGCTGCCAGA AGGCCTTCGT GAAGGCCCTG 
GAGCAGAGGT TCAACCGCGA GCGCCCACAC CTGAGCGAGC TGGGTGCCTG CTTCCTGGAG 
CATCAAGCCG ACTTCCAGAT CTACTCGGAG TACTGCAATA ACCACCCCAA CGCCTGCGTG 
GAGCTCTCCC GGCTCACCAA GCTCAGCAAG TACGTGTACT TCTTCGAGGC CTGCCGGCTG 
CTGCAGAAGA TGATTGACAT CTCCCTGGAT GGCTTCCTGC TGACTCCGGT GCAGAAGATC 
TGCAAGTACC CTCTGCAGCT GGCCGAGCTG CTCAAATACA CGCACCCCCA GCACAGGGAC 
TTCAAGGATG TTGAAGCCGC CTTGCATGCC ATGAAGAACG TGGCCCAGCT CATCAACGAG 
CGGAAGCGGA GACTTGAGAA CATCGACAAG ATTGCTCAGT GGCAGAGCTC CATAGAGGAC 
TGGGAGGGAG AAGATCTCTT GGTCAGGAGC TCAGAACTCA TCTACTCGGG GGAGCTGACT 
CGAGTTACAC AGCCTCAAGC CAAAAGCCAG CAGCGAATGT TCTTTCTCTT TGACCACCAG 
CTCATCTACT GTAAGAAGGA CCTGCTCCGC CGCGACGTGT TGTACTACAA GGGCCGGCTG 
GACATGGACG GCCTGGAGGT GGTGGACCTG GAGGACGGGA AGGACAGAGA CCTCCATGTG 
AGCATCAAGA ACGCCTTCCG GCTGCACCGT GGCGCCACAG GGGACAGCCA CCTGCTGTGC 
ACCAGGAAGC CCGAGCAGAA GCAGCGCTGG CTCAAGGCCT TTGCCAGGGA GAGGGAGCAG 
GTGCAGCTGG ACCAGGAGAC AGGCTTCTCC ATCACTGAAC TGCAGAGGAA GCAGGCCATG 
CTGAATGCCA GCAAGCAGCA GGTCACAGGG AAGCCCAAAG CTGTTGGCCG GCCCTGCTAC 
CTGACGCGCC AGAAGCACCC AGCCCTGCCC AGCAACCGGC CCCAGCAGCA GGTCCTGGTG 
CTGGCGGAGC CCAGGCGCAA GCCATCTACC TTCTGGCACA GCATCAGCCG GCTGGCACCC 
TTCCGCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF4 ( XM_011511277.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509579.1 Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu32422
Clone ID Related Accession (Same CDS sequence)	XM_005263688.3 , XM_005263689.4 , XM_011511277.2 , XM_011511277.1 , XM_005263689.5 , XM_024452938.1 , XM_011511276.2 , XM_005263688.2 , XM_011511276.3
Accession Version	XM_011511277.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011511277.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGACC AGGAGCTGGG CTTCAAAGCT GGGGACGTCA TCGAAGTGAT GGATGCCACC 
AACAGAGAGT GGTGGTGGGG CCGGGTCGCC GATGGCGAGG GCTGGTTTCC AGCCAGCTTC 
GTTCGGCTGA GGGTGAATCA GGACGAGCCC GCGGATGACG ACGCCCCTCT GGCCGGGAAC 
AGCGGAGCGG AGGACGGCGG GGCGGAGGCG CAGAGCAGCA AGGACCAGAT GCGGACCAAC 
GTCATCAACG AGATCCTCAG CACTGAGCGG GACTACATCA AGCACCTGCG CGACATCTGC 
GAGGGCTACG TCCGGCAGTG CCGCAAGCGC GCAGACATGT TCAGCGAGGA GCAGCTGCGT 
ACCATCTTCG GGAACATCGA GGACATCTAC CGCTGCCAGA AGGCCTTCGT GAAGGCCCTG 
GAGCAGAGGT TCAACCGCGA GCGCCCACAC CTGAGCGAGC TGGGTGCCTG CTTCCTGGAG 
CATCAAGCCG ACTTCCAGAT CTACTCGGAG TACTGCAATA ACCACCCCAA CGCCTGCGTG 
GAGCTCTCCC GGCTCACCAA GCTCAGCAAG TACGTGTACT TCTTCGAGGC CTGCCGGCTG 
CTGCAGAAGA TGATTGACAT CTCCCTGGAT GGCTTCCTGC TGACTCCGGT GCAGAAGATC 
TGCAAGTACC CTCTGCAGCT GGCCGAGCTG CTCAAATACA CGCACCCCCA GCACAGGGAC 
TTCAAGGATG TTGAAGCCGC CTTGCATGCC ATGAAGAACG TGGCCCAGCT CATCAACGAG 
CGGAAGCGGA GACTTGAGAA CATCGACAAG ATTGCTCAGT GGCAGAGCTC CATAGAGGAC 
TGGGAGGGAG AAGATCTCTT GGTCAGGAGC TCAGAACTCA TCTACTCGGG GGAGCTGACT 
CGAGTTACAC AGCCTCAAGC CAAAAGCCAG CAGCGAATGT TCTTTCTCTT TGACCACCAG 
CTCATCTACT GTAAGAAGGA CCTGCTCCGC CGCGACGTGT TGTACTACAA GGGCCGGCTG 
GACATGGACG GCCTGGAGGT GGTGGACCTG GAGGACGGGA AGGACAGAGA CCTCCATGTG 
AGCATCAAGA ACGCCTTCCG GCTGCACCGT GGCGCCACAG GGGACAGCCA CCTGCTGTGC 
ACCAGGAAGC CCGAGCAGAA GCAGCGCTGG CTCAAGGCCT TTGCCAGGGA GAGGGAGCAG 
GTGCAGCTGG ACCAGGAGAC AGGCTTCTCC ATCACTGAAC TGCAGAGGAA GCAGGCCATG 
CTGAATGCCA GCAAGCAGCA GGTCACAGGG AAGCCCAAAG CTGTTGGCCG GCCCTGCTAC 
CTGACGCGCC AGAAGCACCC AGCCCTGCCC AGCAACCGGC CCCAGCAGCA GGTCCTGGTG 
CTGGCGGAGC CCAGGCGCAA GCCATCTACC TTCTGGCACA GCATCAGCCG GCTGGCACCC 
TTCCGCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509579.1
CDS	207..1658
Translation	MDDQELGFKAGDVIEVMDATNREWWWGRVADGEGWFPASFVRLRVNQDEPADDDAPLAGNSGAEDGGAEAQSSKDQMRTNVINEILSTERDYIKHLRDICEGYVRQCRKRADMFSEEQLRTIFGNIEDIYRCQKAFVKALEQRFNRERPHLSELGACFLEHQADFQIYSEYCNNHPNACVELSRLTKLSKYVYFFEACRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTHPQHRDFKDVEAALHAMKNVAQLINERKRRLENIDKIAQWQSSIEDWEGEDLLVRSSELIYSGELTRVTQPQAKSQQRMFFLFDHQLIYCKKDLLRRDVLYYKGRLDMDGLEVVDLEDGKDRDLHVSIKNAFRLHRGATGDSHLLCTRKPEQKQRWLKAFAREREQVQLDQETGFSITELQRKQAMLNASKQQVTGKPKAVGRPCYLTRQKHPALPSNRPQQQVLVLAEPRRKPSTFWHSISRLAPFRK

Target ORF information:

RefSeq Version	XM_011511277.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511277.2

ORF Insert Sequence:

ATGGACGACC AGGAGCTGGG CTTCAAAGCT GGGGACGTCA TCGAAGTGAT GGATGCCACC 
AACAGAGAGT GGTGGTGGGG CCGGGTCGCC GATGGCGAGG GCTGGTTTCC AGCCAGCTTC 
GTTCGGCTGA GGGTGAATCA GGACGAGCCC GCGGATGACG ACGCCCCTCT GGCCGGGAAC 
AGCGGAGCGG AGGACGGCGG GGCGGAGGCG CAGAGCAGCA AGGACCAGAT GCGGACCAAC 
GTCATCAACG AGATCCTCAG CACTGAGCGG GACTACATCA AGCACCTGCG CGACATCTGC 
GAGGGCTACG TCCGGCAGTG CCGCAAGCGC GCAGACATGT TCAGCGAGGA GCAGCTGCGT 
ACCATCTTCG GGAACATCGA GGACATCTAC CGCTGCCAGA AGGCCTTCGT GAAGGCCCTG 
GAGCAGAGGT TCAACCGCGA GCGCCCACAC CTGAGCGAGC TGGGTGCCTG CTTCCTGGAG 
CATCAAGCCG ACTTCCAGAT CTACTCGGAG TACTGCAATA ACCACCCCAA CGCCTGCGTG 
GAGCTCTCCC GGCTCACCAA GCTCAGCAAG TACGTGTACT TCTTCGAGGC CTGCCGGCTG 
CTGCAGAAGA TGATTGACAT CTCCCTGGAT GGCTTCCTGC TGACTCCGGT GCAGAAGATC 
TGCAAGTACC CTCTGCAGCT GGCCGAGCTG CTCAAATACA CGCACCCCCA GCACAGGGAC 
TTCAAGGATG TTGAAGCCGC CTTGCATGCC ATGAAGAACG TGGCCCAGCT CATCAACGAG 
CGGAAGCGGA GACTTGAGAA CATCGACAAG ATTGCTCAGT GGCAGAGCTC CATAGAGGAC 
TGGGAGGGAG AAGATCTCTT GGTCAGGAGC TCAGAACTCA TCTACTCGGG GGAGCTGACT 
CGAGTTACAC AGCCTCAAGC CAAAAGCCAG CAGCGAATGT TCTTTCTCTT TGACCACCAG 
CTCATCTACT GTAAGAAGGA CCTGCTCCGC CGCGACGTGT TGTACTACAA GGGCCGGCTG 
GACATGGACG GCCTGGAGGT GGTGGACCTG GAGGACGGGA AGGACAGAGA CCTCCATGTG 
AGCATCAAGA ACGCCTTCCG GCTGCACCGT GGCGCCACAG GGGACAGCCA CCTGCTGTGC 
ACCAGGAAGC CCGAGCAGAA GCAGCGCTGG CTCAAGGCCT TTGCCAGGGA GAGGGAGCAG 
GTGCAGCTGG ACCAGGAGAC AGGCTTCTCC ATCACTGAAC TGCAGAGGAA GCAGGCCATG 
CTGAATGCCA GCAAGCAGCA GGTCACAGGG AAGCCCAAAG CTGTTGGCCG GCCCTGCTAC 
CTGACGCGCC AGAAGCACCC AGCCCTGCCC AGCAACCGGC CCCAGCAGCA GGTCCTGGTG 
CTGGCGGAGC CCAGGCGCAA GCCATCTACC TTCTGGCACA GCATCAGCCG GCTGGCACCC 
TTCCGCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF4 ( XM_011511277.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509579.1 Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu32422
Clone ID Related Accession (Same CDS sequence)	XM_005263688.3 , XM_005263689.4 , XM_011511277.2 , XM_011511277.1 , XM_005263689.5 , XM_024452938.1 , XM_011511276.2 , XM_005263688.2 , XM_011511276.3
Accession Version	XM_011511277.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGACC AGGAGCTGGG CTTCAAAGCT GGGGACGTCA TCGAAGTGAT GGATGCCACC 
AACAGAGAGT GGTGGTGGGG CCGGGTCGCC GATGGCGAGG GCTGGTTTCC AGCCAGCTTC 
GTTCGGCTGA GGGTGAATCA GGACGAGCCC GCGGATGACG ACGCCCCTCT GGCCGGGAAC 
AGCGGAGCGG AGGACGGCGG GGCGGAGGCG CAGAGCAGCA AGGACCAGAT GCGGACCAAC 
GTCATCAACG AGATCCTCAG CACTGAGCGG GACTACATCA AGCACCTGCG CGACATCTGC 
GAGGGCTACG TCCGGCAGTG CCGCAAGCGC GCAGACATGT TCAGCGAGGA GCAGCTGCGT 
ACCATCTTCG GGAACATCGA GGACATCTAC CGCTGCCAGA AGGCCTTCGT GAAGGCCCTG 
GAGCAGAGGT TCAACCGCGA GCGCCCACAC CTGAGCGAGC TGGGTGCCTG CTTCCTGGAG 
CATCAAGCCG ACTTCCAGAT CTACTCGGAG TACTGCAATA ACCACCCCAA CGCCTGCGTG 
GAGCTCTCCC GGCTCACCAA GCTCAGCAAG TACGTGTACT TCTTCGAGGC CTGCCGGCTG 
CTGCAGAAGA TGATTGACAT CTCCCTGGAT GGCTTCCTGC TGACTCCGGT GCAGAAGATC 
TGCAAGTACC CTCTGCAGCT GGCCGAGCTG CTCAAATACA CGCACCCCCA GCACAGGGAC 
TTCAAGGATG TTGAAGCCGC CTTGCATGCC ATGAAGAACG TGGCCCAGCT CATCAACGAG 
CGGAAGCGGA GACTTGAGAA CATCGACAAG ATTGCTCAGT GGCAGAGCTC CATAGAGGAC 
TGGGAGGGAG AAGATCTCTT GGTCAGGAGC TCAGAACTCA TCTACTCGGG GGAGCTGACT 
CGAGTTACAC AGCCTCAAGC CAAAAGCCAG CAGCGAATGT TCTTTCTCTT TGACCACCAG 
CTCATCTACT GTAAGAAGGA CCTGCTCCGC CGCGACGTGT TGTACTACAA GGGCCGGCTG 
GACATGGACG GCCTGGAGGT GGTGGACCTG GAGGACGGGA AGGACAGAGA CCTCCATGTG 
AGCATCAAGA ACGCCTTCCG GCTGCACCGT GGCGCCACAG GGGACAGCCA CCTGCTGTGC 
ACCAGGAAGC CCGAGCAGAA GCAGCGCTGG CTCAAGGCCT TTGCCAGGGA GAGGGAGCAG 
GTGCAGCTGG ACCAGGAGAC AGGCTTCTCC ATCACTGAAC TGCAGAGGAA GCAGGCCATG 
CTGAATGCCA GCAAGCAGCA GGTCACAGGG AAGCCCAAAG CTGTTGGCCG GCCCTGCTAC 
CTGACGCGCC AGAAGCACCC AGCCCTGCCC AGCAACCGGC CCCAGCAGCA GGTCCTGGTG 
CTGGCGGAGC CCAGGCGCAA GCCATCTACC TTCTGGCACA GCATCAGCCG GCTGGCACCC 
TTCCGCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509579.1
CDS	207..1658
Translation	MDDQELGFKAGDVIEVMDATNREWWWGRVADGEGWFPASFVRLRVNQDEPADDDAPLAGNSGAEDGGAEAQSSKDQMRTNVINEILSTERDYIKHLRDICEGYVRQCRKRADMFSEEQLRTIFGNIEDIYRCQKAFVKALEQRFNRERPHLSELGACFLEHQADFQIYSEYCNNHPNACVELSRLTKLSKYVYFFEACRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTHPQHRDFKDVEAALHAMKNVAQLINERKRRLENIDKIAQWQSSIEDWEGEDLLVRSSELIYSGELTRVTQPQAKSQQRMFFLFDHQLIYCKKDLLRRDVLYYKGRLDMDGLEVVDLEDGKDRDLHVSIKNAFRLHRGATGDSHLLCTRKPEQKQRWLKAFAREREQVQLDQETGFSITELQRKQAMLNASKQQVTGKPKAVGRPCYLTRQKHPALPSNRPQQQVLVLAEPRRKPSTFWHSISRLAPFRK

Target ORF information:

RefSeq Version	XM_011511277.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511277.1

ORF Insert Sequence:

ATGGACGACC AGGAGCTGGG CTTCAAAGCT GGGGACGTCA TCGAAGTGAT GGATGCCACC 
AACAGAGAGT GGTGGTGGGG CCGGGTCGCC GATGGCGAGG GCTGGTTTCC AGCCAGCTTC 
GTTCGGCTGA GGGTGAATCA GGACGAGCCC GCGGATGACG ACGCCCCTCT GGCCGGGAAC 
AGCGGAGCGG AGGACGGCGG GGCGGAGGCG CAGAGCAGCA AGGACCAGAT GCGGACCAAC 
GTCATCAACG AGATCCTCAG CACTGAGCGG GACTACATCA AGCACCTGCG CGACATCTGC 
GAGGGCTACG TCCGGCAGTG CCGCAAGCGC GCAGACATGT TCAGCGAGGA GCAGCTGCGT 
ACCATCTTCG GGAACATCGA GGACATCTAC CGCTGCCAGA AGGCCTTCGT GAAGGCCCTG 
GAGCAGAGGT TCAACCGCGA GCGCCCACAC CTGAGCGAGC TGGGTGCCTG CTTCCTGGAG 
CATCAAGCCG ACTTCCAGAT CTACTCGGAG TACTGCAATA ACCACCCCAA CGCCTGCGTG 
GAGCTCTCCC GGCTCACCAA GCTCAGCAAG TACGTGTACT TCTTCGAGGC CTGCCGGCTG 
CTGCAGAAGA TGATTGACAT CTCCCTGGAT GGCTTCCTGC TGACTCCGGT GCAGAAGATC 
TGCAAGTACC CTCTGCAGCT GGCCGAGCTG CTCAAATACA CGCACCCCCA GCACAGGGAC 
TTCAAGGATG TTGAAGCCGC CTTGCATGCC ATGAAGAACG TGGCCCAGCT CATCAACGAG 
CGGAAGCGGA GACTTGAGAA CATCGACAAG ATTGCTCAGT GGCAGAGCTC CATAGAGGAC 
TGGGAGGGAG AAGATCTCTT GGTCAGGAGC TCAGAACTCA TCTACTCGGG GGAGCTGACT 
CGAGTTACAC AGCCTCAAGC CAAAAGCCAG CAGCGAATGT TCTTTCTCTT TGACCACCAG 
CTCATCTACT GTAAGAAGGA CCTGCTCCGC CGCGACGTGT TGTACTACAA GGGCCGGCTG 
GACATGGACG GCCTGGAGGT GGTGGACCTG GAGGACGGGA AGGACAGAGA CCTCCATGTG 
AGCATCAAGA ACGCCTTCCG GCTGCACCGT GGCGCCACAG GGGACAGCCA CCTGCTGTGC 
ACCAGGAAGC CCGAGCAGAA GCAGCGCTGG CTCAAGGCCT TTGCCAGGGA GAGGGAGCAG 
GTGCAGCTGG ACCAGGAGAC AGGCTTCTCC ATCACTGAAC TGCAGAGGAA GCAGGCCATG 
CTGAATGCCA GCAAGCAGCA GGTCACAGGG AAGCCCAAAG CTGTTGGCCG GCCCTGCTAC 
CTGACGCGCC AGAAGCACCC AGCCCTGCCC AGCAACCGGC CCCAGCAGCA GGTCCTGGTG 
CTGGCGGAGC CCAGGCGCAA GCCATCTACC TTCTGGCACA GCATCAGCCG GCTGGCACCC 
TTCCGCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF4 ( XM_005263689.5 ) cDNA ORF clone, Homo sapiens(human) -> XP_005263746.1 Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu32422
Clone ID Related Accession (Same CDS sequence)	XM_005263688.3 , XM_005263689.4 , XM_011511277.2 , XM_011511277.1 , XM_005263689.5 , XM_024452938.1 , XM_011511276.2 , XM_005263688.2 , XM_011511276.3
Accession Version	XM_005263689.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005263689.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGACC AGGAGCTGGG CTTCAAAGCT GGGGACGTCA TCGAAGTGAT GGATGCCACC 
AACAGAGAGT GGTGGTGGGG CCGGGTCGCC GATGGCGAGG GCTGGTTTCC AGCCAGCTTC 
GTTCGGCTGA GGGTGAATCA GGACGAGCCC GCGGATGACG ACGCCCCTCT GGCCGGGAAC 
AGCGGAGCGG AGGACGGCGG GGCGGAGGCG CAGAGCAGCA AGGACCAGAT GCGGACCAAC 
GTCATCAACG AGATCCTCAG CACTGAGCGG GACTACATCA AGCACCTGCG CGACATCTGC 
GAGGGCTACG TCCGGCAGTG CCGCAAGCGC GCAGACATGT TCAGCGAGGA GCAGCTGCGT 
ACCATCTTCG GGAACATCGA GGACATCTAC CGCTGCCAGA AGGCCTTCGT GAAGGCCCTG 
GAGCAGAGGT TCAACCGCGA GCGCCCACAC CTGAGCGAGC TGGGTGCCTG CTTCCTGGAG 
CATCAAGCCG ACTTCCAGAT CTACTCGGAG TACTGCAATA ACCACCCCAA CGCCTGCGTG 
GAGCTCTCCC GGCTCACCAA GCTCAGCAAG TACGTGTACT TCTTCGAGGC CTGCCGGCTG 
CTGCAGAAGA TGATTGACAT CTCCCTGGAT GGCTTCCTGC TGACTCCGGT GCAGAAGATC 
TGCAAGTACC CTCTGCAGCT GGCCGAGCTG CTCAAATACA CGCACCCCCA GCACAGGGAC 
TTCAAGGATG TTGAAGCCGC CTTGCATGCC ATGAAGAACG TGGCCCAGCT CATCAACGAG 
CGGAAGCGGA GACTTGAGAA CATCGACAAG ATTGCTCAGT GGCAGAGCTC CATAGAGGAC 
TGGGAGGGAG AAGATCTCTT GGTCAGGAGC TCAGAACTCA TCTACTCGGG GGAGCTGACT 
CGAGTTACAC AGCCTCAAGC CAAAAGCCAG CAGCGAATGT TCTTTCTCTT TGACCACCAG 
CTCATCTACT GTAAGAAGGA CCTGCTCCGC CGCGACGTGT TGTACTACAA GGGCCGGCTG 
GACATGGACG GCCTGGAGGT GGTGGACCTG GAGGACGGGA AGGACAGAGA CCTCCATGTG 
AGCATCAAGA ACGCCTTCCG GCTGCACCGT GGCGCCACAG GGGACAGCCA CCTGCTGTGC 
ACCAGGAAGC CCGAGCAGAA GCAGCGCTGG CTCAAGGCCT TTGCCAGGGA GAGGGAGCAG 
GTGCAGCTGG ACCAGGAGAC AGGCTTCTCC ATCACTGAAC TGCAGAGGAA GCAGGCCATG 
CTGAATGCCA GCAAGCAGCA GGTCACAGGG AAGCCCAAAG CTGTTGGCCG GCCCTGCTAC 
CTGACGCGCC AGAAGCACCC AGCCCTGCCC AGCAACCGGC CCCAGCAGCA GGTCCTGGTG 
CTGGCGGAGC CCAGGCGCAA GCCATCTACC TTCTGGCACA GCATCAGCCG GCTGGCACCC 
TTCCGCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005263746.1
CDS	375..1826
Translation	MDDQELGFKAGDVIEVMDATNREWWWGRVADGEGWFPASFVRLRVNQDEPADDDAPLAGNSGAEDGGAEAQSSKDQMRTNVINEILSTERDYIKHLRDICEGYVRQCRKRADMFSEEQLRTIFGNIEDIYRCQKAFVKALEQRFNRERPHLSELGACFLEHQADFQIYSEYCNNHPNACVELSRLTKLSKYVYFFEACRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTHPQHRDFKDVEAALHAMKNVAQLINERKRRLENIDKIAQWQSSIEDWEGEDLLVRSSELIYSGELTRVTQPQAKSQQRMFFLFDHQLIYCKKDLLRRDVLYYKGRLDMDGLEVVDLEDGKDRDLHVSIKNAFRLHRGATGDSHLLCTRKPEQKQRWLKAFAREREQVQLDQETGFSITELQRKQAMLNASKQQVTGKPKAVGRPCYLTRQKHPALPSNRPQQQVLVLAEPRRKPSTFWHSISRLAPFRK

Target ORF information:

RefSeq Version	XM_005263689.5
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005263689.5

ORF Insert Sequence:

ATGGACGACC AGGAGCTGGG CTTCAAAGCT GGGGACGTCA TCGAAGTGAT GGATGCCACC 
AACAGAGAGT GGTGGTGGGG CCGGGTCGCC GATGGCGAGG GCTGGTTTCC AGCCAGCTTC 
GTTCGGCTGA GGGTGAATCA GGACGAGCCC GCGGATGACG ACGCCCCTCT GGCCGGGAAC 
AGCGGAGCGG AGGACGGCGG GGCGGAGGCG CAGAGCAGCA AGGACCAGAT GCGGACCAAC 
GTCATCAACG AGATCCTCAG CACTGAGCGG GACTACATCA AGCACCTGCG CGACATCTGC 
GAGGGCTACG TCCGGCAGTG CCGCAAGCGC GCAGACATGT TCAGCGAGGA GCAGCTGCGT 
ACCATCTTCG GGAACATCGA GGACATCTAC CGCTGCCAGA AGGCCTTCGT GAAGGCCCTG 
GAGCAGAGGT TCAACCGCGA GCGCCCACAC CTGAGCGAGC TGGGTGCCTG CTTCCTGGAG 
CATCAAGCCG ACTTCCAGAT CTACTCGGAG TACTGCAATA ACCACCCCAA CGCCTGCGTG 
GAGCTCTCCC GGCTCACCAA GCTCAGCAAG TACGTGTACT TCTTCGAGGC CTGCCGGCTG 
CTGCAGAAGA TGATTGACAT CTCCCTGGAT GGCTTCCTGC TGACTCCGGT GCAGAAGATC 
TGCAAGTACC CTCTGCAGCT GGCCGAGCTG CTCAAATACA CGCACCCCCA GCACAGGGAC 
TTCAAGGATG TTGAAGCCGC CTTGCATGCC ATGAAGAACG TGGCCCAGCT CATCAACGAG 
CGGAAGCGGA GACTTGAGAA CATCGACAAG ATTGCTCAGT GGCAGAGCTC CATAGAGGAC 
TGGGAGGGAG AAGATCTCTT GGTCAGGAGC TCAGAACTCA TCTACTCGGG GGAGCTGACT 
CGAGTTACAC AGCCTCAAGC CAAAAGCCAG CAGCGAATGT TCTTTCTCTT TGACCACCAG 
CTCATCTACT GTAAGAAGGA CCTGCTCCGC CGCGACGTGT TGTACTACAA GGGCCGGCTG 
GACATGGACG GCCTGGAGGT GGTGGACCTG GAGGACGGGA AGGACAGAGA CCTCCATGTG 
AGCATCAAGA ACGCCTTCCG GCTGCACCGT GGCGCCACAG GGGACAGCCA CCTGCTGTGC 
ACCAGGAAGC CCGAGCAGAA GCAGCGCTGG CTCAAGGCCT TTGCCAGGGA GAGGGAGCAG 
GTGCAGCTGG ACCAGGAGAC AGGCTTCTCC ATCACTGAAC TGCAGAGGAA GCAGGCCATG 
CTGAATGCCA GCAAGCAGCA GGTCACAGGG AAGCCCAAAG CTGTTGGCCG GCCCTGCTAC 
CTGACGCGCC AGAAGCACCC AGCCCTGCCC AGCAACCGGC CCCAGCAGCA GGTCCTGGTG 
CTGGCGGAGC CCAGGCGCAA GCCATCTACC TTCTGGCACA GCATCAGCCG GCTGGCACCC 
TTCCGCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF4 ( XM_024452938.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308706.1 Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu32422
Clone ID Related Accession (Same CDS sequence)	XM_005263688.3 , XM_005263689.4 , XM_011511277.2 , XM_011511277.1 , XM_005263689.5 , XM_024452938.1 , XM_011511276.2 , XM_005263688.2 , XM_011511276.3
Accession Version	XM_024452938.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGACC AGGAGCTGGG CTTCAAAGCT GGGGACGTCA TCGAAGTGAT GGATGCCACC 
AACAGAGAGT GGTGGTGGGG CCGGGTCGCC GATGGCGAGG GCTGGTTTCC AGCCAGCTTC 
GTTCGGCTGA GGGTGAATCA GGACGAGCCC GCGGATGACG ACGCCCCTCT GGCCGGGAAC 
AGCGGAGCGG AGGACGGCGG GGCGGAGGCG CAGAGCAGCA AGGACCAGAT GCGGACCAAC 
GTCATCAACG AGATCCTCAG CACTGAGCGG GACTACATCA AGCACCTGCG CGACATCTGC 
GAGGGCTACG TCCGGCAGTG CCGCAAGCGC GCAGACATGT TCAGCGAGGA GCAGCTGCGT 
ACCATCTTCG GGAACATCGA GGACATCTAC CGCTGCCAGA AGGCCTTCGT GAAGGCCCTG 
GAGCAGAGGT TCAACCGCGA GCGCCCACAC CTGAGCGAGC TGGGTGCCTG CTTCCTGGAG 
CATCAAGCCG ACTTCCAGAT CTACTCGGAG TACTGCAATA ACCACCCCAA CGCCTGCGTG 
GAGCTCTCCC GGCTCACCAA GCTCAGCAAG TACGTGTACT TCTTCGAGGC CTGCCGGCTG 
CTGCAGAAGA TGATTGACAT CTCCCTGGAT GGCTTCCTGC TGACTCCGGT GCAGAAGATC 
TGCAAGTACC CTCTGCAGCT GGCCGAGCTG CTCAAATACA CGCACCCCCA GCACAGGGAC 
TTCAAGGATG TTGAAGCCGC CTTGCATGCC ATGAAGAACG TGGCCCAGCT CATCAACGAG 
CGGAAGCGGA GACTTGAGAA CATCGACAAG ATTGCTCAGT GGCAGAGCTC CATAGAGGAC 
TGGGAGGGAG AAGATCTCTT GGTCAGGAGC TCAGAACTCA TCTACTCGGG GGAGCTGACT 
CGAGTTACAC AGCCTCAAGC CAAAAGCCAG CAGCGAATGT TCTTTCTCTT TGACCACCAG 
CTCATCTACT GTAAGAAGGA CCTGCTCCGC CGCGACGTGT TGTACTACAA GGGCCGGCTG 
GACATGGACG GCCTGGAGGT GGTGGACCTG GAGGACGGGA AGGACAGAGA CCTCCATGTG 
AGCATCAAGA ACGCCTTCCG GCTGCACCGT GGCGCCACAG GGGACAGCCA CCTGCTGTGC 
ACCAGGAAGC CCGAGCAGAA GCAGCGCTGG CTCAAGGCCT TTGCCAGGGA GAGGGAGCAG 
GTGCAGCTGG ACCAGGAGAC AGGCTTCTCC ATCACTGAAC TGCAGAGGAA GCAGGCCATG 
CTGAATGCCA GCAAGCAGCA GGTCACAGGG AAGCCCAAAG CTGTTGGCCG GCCCTGCTAC 
CTGACGCGCC AGAAGCACCC AGCCCTGCCC AGCAACCGGC CCCAGCAGCA GGTCCTGGTG 
CTGGCGGAGC CCAGGCGCAA GCCATCTACC TTCTGGCACA GCATCAGCCG GCTGGCACCC 
TTCCGCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308706.1
CDS	221..1672
Translation	MDDQELGFKAGDVIEVMDATNREWWWGRVADGEGWFPASFVRLRVNQDEPADDDAPLAGNSGAEDGGAEAQSSKDQMRTNVINEILSTERDYIKHLRDICEGYVRQCRKRADMFSEEQLRTIFGNIEDIYRCQKAFVKALEQRFNRERPHLSELGACFLEHQADFQIYSEYCNNHPNACVELSRLTKLSKYVYFFEACRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTHPQHRDFKDVEAALHAMKNVAQLINERKRRLENIDKIAQWQSSIEDWEGEDLLVRSSELIYSGELTRVTQPQAKSQQRMFFLFDHQLIYCKKDLLRRDVLYYKGRLDMDGLEVVDLEDGKDRDLHVSIKNAFRLHRGATGDSHLLCTRKPEQKQRWLKAFAREREQVQLDQETGFSITELQRKQAMLNASKQQVTGKPKAVGRPCYLTRQKHPALPSNRPQQQVLVLAEPRRKPSTFWHSISRLAPFRK

Target ORF information:

RefSeq Version	XM_024452938.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024452938.1

ORF Insert Sequence:

ATGGACGACC AGGAGCTGGG CTTCAAAGCT GGGGACGTCA TCGAAGTGAT GGATGCCACC 
AACAGAGAGT GGTGGTGGGG CCGGGTCGCC GATGGCGAGG GCTGGTTTCC AGCCAGCTTC 
GTTCGGCTGA GGGTGAATCA GGACGAGCCC GCGGATGACG ACGCCCCTCT GGCCGGGAAC 
AGCGGAGCGG AGGACGGCGG GGCGGAGGCG CAGAGCAGCA AGGACCAGAT GCGGACCAAC 
GTCATCAACG AGATCCTCAG CACTGAGCGG GACTACATCA AGCACCTGCG CGACATCTGC 
GAGGGCTACG TCCGGCAGTG CCGCAAGCGC GCAGACATGT TCAGCGAGGA GCAGCTGCGT 
ACCATCTTCG GGAACATCGA GGACATCTAC CGCTGCCAGA AGGCCTTCGT GAAGGCCCTG 
GAGCAGAGGT TCAACCGCGA GCGCCCACAC CTGAGCGAGC TGGGTGCCTG CTTCCTGGAG 
CATCAAGCCG ACTTCCAGAT CTACTCGGAG TACTGCAATA ACCACCCCAA CGCCTGCGTG 
GAGCTCTCCC GGCTCACCAA GCTCAGCAAG TACGTGTACT TCTTCGAGGC CTGCCGGCTG 
CTGCAGAAGA TGATTGACAT CTCCCTGGAT GGCTTCCTGC TGACTCCGGT GCAGAAGATC 
TGCAAGTACC CTCTGCAGCT GGCCGAGCTG CTCAAATACA CGCACCCCCA GCACAGGGAC 
TTCAAGGATG TTGAAGCCGC CTTGCATGCC ATGAAGAACG TGGCCCAGCT CATCAACGAG 
CGGAAGCGGA GACTTGAGAA CATCGACAAG ATTGCTCAGT GGCAGAGCTC CATAGAGGAC 
TGGGAGGGAG AAGATCTCTT GGTCAGGAGC TCAGAACTCA TCTACTCGGG GGAGCTGACT 
CGAGTTACAC AGCCTCAAGC CAAAAGCCAG CAGCGAATGT TCTTTCTCTT TGACCACCAG 
CTCATCTACT GTAAGAAGGA CCTGCTCCGC CGCGACGTGT TGTACTACAA GGGCCGGCTG 
GACATGGACG GCCTGGAGGT GGTGGACCTG GAGGACGGGA AGGACAGAGA CCTCCATGTG 
AGCATCAAGA ACGCCTTCCG GCTGCACCGT GGCGCCACAG GGGACAGCCA CCTGCTGTGC 
ACCAGGAAGC CCGAGCAGAA GCAGCGCTGG CTCAAGGCCT TTGCCAGGGA GAGGGAGCAG 
GTGCAGCTGG ACCAGGAGAC AGGCTTCTCC ATCACTGAAC TGCAGAGGAA GCAGGCCATG 
CTGAATGCCA GCAAGCAGCA GGTCACAGGG AAGCCCAAAG CTGTTGGCCG GCCCTGCTAC 
CTGACGCGCC AGAAGCACCC AGCCCTGCCC AGCAACCGGC CCCAGCAGCA GGTCCTGGTG 
CTGGCGGAGC CCAGGCGCAA GCCATCTACC TTCTGGCACA GCATCAGCCG GCTGGCACCC 
TTCCGCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF4 ( XM_011511276.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509578.1 Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu32422
Clone ID Related Accession (Same CDS sequence)	XM_005263688.3 , XM_005263689.4 , XM_011511277.2 , XM_011511277.1 , XM_005263689.5 , XM_024452938.1 , XM_011511276.2 , XM_005263688.2 , XM_011511276.3
Accession Version	XM_011511276.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011511276.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGACC AGGAGCTGGG CTTCAAAGCT GGGGACGTCA TCGAAGTGAT GGATGCCACC 
AACAGAGAGT GGTGGTGGGG CCGGGTCGCC GATGGCGAGG GCTGGTTTCC AGCCAGCTTC 
GTTCGGCTGA GGGTGAATCA GGACGAGCCC GCGGATGACG ACGCCCCTCT GGCCGGGAAC 
AGCGGAGCGG AGGACGGCGG GGCGGAGGCG CAGAGCAGCA AGGACCAGAT GCGGACCAAC 
GTCATCAACG AGATCCTCAG CACTGAGCGG GACTACATCA AGCACCTGCG CGACATCTGC 
GAGGGCTACG TCCGGCAGTG CCGCAAGCGC GCAGACATGT TCAGCGAGGA GCAGCTGCGT 
ACCATCTTCG GGAACATCGA GGACATCTAC CGCTGCCAGA AGGCCTTCGT GAAGGCCCTG 
GAGCAGAGGT TCAACCGCGA GCGCCCACAC CTGAGCGAGC TGGGTGCCTG CTTCCTGGAG 
CATCAAGCCG ACTTCCAGAT CTACTCGGAG TACTGCAATA ACCACCCCAA CGCCTGCGTG 
GAGCTCTCCC GGCTCACCAA GCTCAGCAAG TACGTGTACT TCTTCGAGGC CTGCCGGCTG 
CTGCAGAAGA TGATTGACAT CTCCCTGGAT GGCTTCCTGC TGACTCCGGT GCAGAAGATC 
TGCAAGTACC CTCTGCAGCT GGCCGAGCTG CTCAAATACA CGCACCCCCA GCACAGGGAC 
TTCAAGGATG TTGAAGCCGC CTTGCATGCC ATGAAGAACG TGGCCCAGCT CATCAACGAG 
CGGAAGCGGA GACTTGAGAA CATCGACAAG ATTGCTCAGT GGCAGAGCTC CATAGAGGAC 
TGGGAGGGAG AAGATCTCTT GGTCAGGAGC TCAGAACTCA TCTACTCGGG GGAGCTGACT 
CGAGTTACAC AGCCTCAAGC CAAAAGCCAG CAGCGAATGT TCTTTCTCTT TGACCACCAG 
CTCATCTACT GTAAGAAGGA CCTGCTCCGC CGCGACGTGT TGTACTACAA GGGCCGGCTG 
GACATGGACG GCCTGGAGGT GGTGGACCTG GAGGACGGGA AGGACAGAGA CCTCCATGTG 
AGCATCAAGA ACGCCTTCCG GCTGCACCGT GGCGCCACAG GGGACAGCCA CCTGCTGTGC 
ACCAGGAAGC CCGAGCAGAA GCAGCGCTGG CTCAAGGCCT TTGCCAGGGA GAGGGAGCAG 
GTGCAGCTGG ACCAGGAGAC AGGCTTCTCC ATCACTGAAC TGCAGAGGAA GCAGGCCATG 
CTGAATGCCA GCAAGCAGCA GGTCACAGGG AAGCCCAAAG CTGTTGGCCG GCCCTGCTAC 
CTGACGCGCC AGAAGCACCC AGCCCTGCCC AGCAACCGGC CCCAGCAGCA GGTCCTGGTG 
CTGGCGGAGC CCAGGCGCAA GCCATCTACC TTCTGGCACA GCATCAGCCG GCTGGCACCC 
TTCCGCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509578.1
CDS	80..1531
Translation	MDDQELGFKAGDVIEVMDATNREWWWGRVADGEGWFPASFVRLRVNQDEPADDDAPLAGNSGAEDGGAEAQSSKDQMRTNVINEILSTERDYIKHLRDICEGYVRQCRKRADMFSEEQLRTIFGNIEDIYRCQKAFVKALEQRFNRERPHLSELGACFLEHQADFQIYSEYCNNHPNACVELSRLTKLSKYVYFFEACRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTHPQHRDFKDVEAALHAMKNVAQLINERKRRLENIDKIAQWQSSIEDWEGEDLLVRSSELIYSGELTRVTQPQAKSQQRMFFLFDHQLIYCKKDLLRRDVLYYKGRLDMDGLEVVDLEDGKDRDLHVSIKNAFRLHRGATGDSHLLCTRKPEQKQRWLKAFAREREQVQLDQETGFSITELQRKQAMLNASKQQVTGKPKAVGRPCYLTRQKHPALPSNRPQQQVLVLAEPRRKPSTFWHSISRLAPFRK

Target ORF information:

RefSeq Version	XM_011511276.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511276.2

ORF Insert Sequence:

ATGGACGACC AGGAGCTGGG CTTCAAAGCT GGGGACGTCA TCGAAGTGAT GGATGCCACC 
AACAGAGAGT GGTGGTGGGG CCGGGTCGCC GATGGCGAGG GCTGGTTTCC AGCCAGCTTC 
GTTCGGCTGA GGGTGAATCA GGACGAGCCC GCGGATGACG ACGCCCCTCT GGCCGGGAAC 
AGCGGAGCGG AGGACGGCGG GGCGGAGGCG CAGAGCAGCA AGGACCAGAT GCGGACCAAC 
GTCATCAACG AGATCCTCAG CACTGAGCGG GACTACATCA AGCACCTGCG CGACATCTGC 
GAGGGCTACG TCCGGCAGTG CCGCAAGCGC GCAGACATGT TCAGCGAGGA GCAGCTGCGT 
ACCATCTTCG GGAACATCGA GGACATCTAC CGCTGCCAGA AGGCCTTCGT GAAGGCCCTG 
GAGCAGAGGT TCAACCGCGA GCGCCCACAC CTGAGCGAGC TGGGTGCCTG CTTCCTGGAG 
CATCAAGCCG ACTTCCAGAT CTACTCGGAG TACTGCAATA ACCACCCCAA CGCCTGCGTG 
GAGCTCTCCC GGCTCACCAA GCTCAGCAAG TACGTGTACT TCTTCGAGGC CTGCCGGCTG 
CTGCAGAAGA TGATTGACAT CTCCCTGGAT GGCTTCCTGC TGACTCCGGT GCAGAAGATC 
TGCAAGTACC CTCTGCAGCT GGCCGAGCTG CTCAAATACA CGCACCCCCA GCACAGGGAC 
TTCAAGGATG TTGAAGCCGC CTTGCATGCC ATGAAGAACG TGGCCCAGCT CATCAACGAG 
CGGAAGCGGA GACTTGAGAA CATCGACAAG ATTGCTCAGT GGCAGAGCTC CATAGAGGAC 
TGGGAGGGAG AAGATCTCTT GGTCAGGAGC TCAGAACTCA TCTACTCGGG GGAGCTGACT 
CGAGTTACAC AGCCTCAAGC CAAAAGCCAG CAGCGAATGT TCTTTCTCTT TGACCACCAG 
CTCATCTACT GTAAGAAGGA CCTGCTCCGC CGCGACGTGT TGTACTACAA GGGCCGGCTG 
GACATGGACG GCCTGGAGGT GGTGGACCTG GAGGACGGGA AGGACAGAGA CCTCCATGTG 
AGCATCAAGA ACGCCTTCCG GCTGCACCGT GGCGCCACAG GGGACAGCCA CCTGCTGTGC 
ACCAGGAAGC CCGAGCAGAA GCAGCGCTGG CTCAAGGCCT TTGCCAGGGA GAGGGAGCAG 
GTGCAGCTGG ACCAGGAGAC AGGCTTCTCC ATCACTGAAC TGCAGAGGAA GCAGGCCATG 
CTGAATGCCA GCAAGCAGCA GGTCACAGGG AAGCCCAAAG CTGTTGGCCG GCCCTGCTAC 
CTGACGCGCC AGAAGCACCC AGCCCTGCCC AGCAACCGGC CCCAGCAGCA GGTCCTGGTG 
CTGGCGGAGC CCAGGCGCAA GCCATCTACC TTCTGGCACA GCATCAGCCG GCTGGCACCC 
TTCCGCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF4 ( XM_005263688.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005263745.1 Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu32422
Clone ID Related Accession (Same CDS sequence)	XM_005263688.3 , XM_005263689.4 , XM_011511277.2 , XM_011511277.1 , XM_005263689.5 , XM_024452938.1 , XM_011511276.2 , XM_005263688.2 , XM_011511276.3
Accession Version	XM_005263688.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005263688.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGACC AGGAGCTGGG CTTCAAAGCT GGGGACGTCA TCGAAGTGAT GGATGCCACC 
AACAGAGAGT GGTGGTGGGG CCGGGTCGCC GATGGCGAGG GCTGGTTTCC AGCCAGCTTC 
GTTCGGCTGA GGGTGAATCA GGACGAGCCC GCGGATGACG ACGCCCCTCT GGCCGGGAAC 
AGCGGAGCGG AGGACGGCGG GGCGGAGGCG CAGAGCAGCA AGGACCAGAT GCGGACCAAC 
GTCATCAACG AGATCCTCAG CACTGAGCGG GACTACATCA AGCACCTGCG CGACATCTGC 
GAGGGCTACG TCCGGCAGTG CCGCAAGCGC GCAGACATGT TCAGCGAGGA GCAGCTGCGT 
ACCATCTTCG GGAACATCGA GGACATCTAC CGCTGCCAGA AGGCCTTCGT GAAGGCCCTG 
GAGCAGAGGT TCAACCGCGA GCGCCCACAC CTGAGCGAGC TGGGTGCCTG CTTCCTGGAG 
CATCAAGCCG ACTTCCAGAT CTACTCGGAG TACTGCAATA ACCACCCCAA CGCCTGCGTG 
GAGCTCTCCC GGCTCACCAA GCTCAGCAAG TACGTGTACT TCTTCGAGGC CTGCCGGCTG 
CTGCAGAAGA TGATTGACAT CTCCCTGGAT GGCTTCCTGC TGACTCCGGT GCAGAAGATC 
TGCAAGTACC CTCTGCAGCT GGCCGAGCTG CTCAAATACA CGCACCCCCA GCACAGGGAC 
TTCAAGGATG TTGAAGCCGC CTTGCATGCC ATGAAGAACG TGGCCCAGCT CATCAACGAG 
CGGAAGCGGA GACTTGAGAA CATCGACAAG ATTGCTCAGT GGCAGAGCTC CATAGAGGAC 
TGGGAGGGAG AAGATCTCTT GGTCAGGAGC TCAGAACTCA TCTACTCGGG GGAGCTGACT 
CGAGTTACAC AGCCTCAAGC CAAAAGCCAG CAGCGAATGT TCTTTCTCTT TGACCACCAG 
CTCATCTACT GTAAGAAGGA CCTGCTCCGC CGCGACGTGT TGTACTACAA GGGCCGGCTG 
GACATGGACG GCCTGGAGGT GGTGGACCTG GAGGACGGGA AGGACAGAGA CCTCCATGTG 
AGCATCAAGA ACGCCTTCCG GCTGCACCGT GGCGCCACAG GGGACAGCCA CCTGCTGTGC 
ACCAGGAAGC CCGAGCAGAA GCAGCGCTGG CTCAAGGCCT TTGCCAGGGA GAGGGAGCAG 
GTGCAGCTGG ACCAGGAGAC AGGCTTCTCC ATCACTGAAC TGCAGAGGAA GCAGGCCATG 
CTGAATGCCA GCAAGCAGCA GGTCACAGGG AAGCCCAAAG CTGTTGGCCG GCCCTGCTAC 
CTGACGCGCC AGAAGCACCC AGCCCTGCCC AGCAACCGGC CCCAGCAGCA GGTCCTGGTG 
CTGGCGGAGC CCAGGCGCAA GCCATCTACC TTCTGGCACA GCATCAGCCG GCTGGCACCC 
TTCCGCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005263745.1
CDS	362..1813
Translation	MDDQELGFKAGDVIEVMDATNREWWWGRVADGEGWFPASFVRLRVNQDEPADDDAPLAGNSGAEDGGAEAQSSKDQMRTNVINEILSTERDYIKHLRDICEGYVRQCRKRADMFSEEQLRTIFGNIEDIYRCQKAFVKALEQRFNRERPHLSELGACFLEHQADFQIYSEYCNNHPNACVELSRLTKLSKYVYFFEACRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTHPQHRDFKDVEAALHAMKNVAQLINERKRRLENIDKIAQWQSSIEDWEGEDLLVRSSELIYSGELTRVTQPQAKSQQRMFFLFDHQLIYCKKDLLRRDVLYYKGRLDMDGLEVVDLEDGKDRDLHVSIKNAFRLHRGATGDSHLLCTRKPEQKQRWLKAFAREREQVQLDQETGFSITELQRKQAMLNASKQQVTGKPKAVGRPCYLTRQKHPALPSNRPQQQVLVLAEPRRKPSTFWHSISRLAPFRK

Target ORF information:

RefSeq Version	XM_005263688.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005263688.2

ORF Insert Sequence:

ATGGACGACC AGGAGCTGGG CTTCAAAGCT GGGGACGTCA TCGAAGTGAT GGATGCCACC 
AACAGAGAGT GGTGGTGGGG CCGGGTCGCC GATGGCGAGG GCTGGTTTCC AGCCAGCTTC 
GTTCGGCTGA GGGTGAATCA GGACGAGCCC GCGGATGACG ACGCCCCTCT GGCCGGGAAC 
AGCGGAGCGG AGGACGGCGG GGCGGAGGCG CAGAGCAGCA AGGACCAGAT GCGGACCAAC 
GTCATCAACG AGATCCTCAG CACTGAGCGG GACTACATCA AGCACCTGCG CGACATCTGC 
GAGGGCTACG TCCGGCAGTG CCGCAAGCGC GCAGACATGT TCAGCGAGGA GCAGCTGCGT 
ACCATCTTCG GGAACATCGA GGACATCTAC CGCTGCCAGA AGGCCTTCGT GAAGGCCCTG 
GAGCAGAGGT TCAACCGCGA GCGCCCACAC CTGAGCGAGC TGGGTGCCTG CTTCCTGGAG 
CATCAAGCCG ACTTCCAGAT CTACTCGGAG TACTGCAATA ACCACCCCAA CGCCTGCGTG 
GAGCTCTCCC GGCTCACCAA GCTCAGCAAG TACGTGTACT TCTTCGAGGC CTGCCGGCTG 
CTGCAGAAGA TGATTGACAT CTCCCTGGAT GGCTTCCTGC TGACTCCGGT GCAGAAGATC 
TGCAAGTACC CTCTGCAGCT GGCCGAGCTG CTCAAATACA CGCACCCCCA GCACAGGGAC 
TTCAAGGATG TTGAAGCCGC CTTGCATGCC ATGAAGAACG TGGCCCAGCT CATCAACGAG 
CGGAAGCGGA GACTTGAGAA CATCGACAAG ATTGCTCAGT GGCAGAGCTC CATAGAGGAC 
TGGGAGGGAG AAGATCTCTT GGTCAGGAGC TCAGAACTCA TCTACTCGGG GGAGCTGACT 
CGAGTTACAC AGCCTCAAGC CAAAAGCCAG CAGCGAATGT TCTTTCTCTT TGACCACCAG 
CTCATCTACT GTAAGAAGGA CCTGCTCCGC CGCGACGTGT TGTACTACAA GGGCCGGCTG 
GACATGGACG GCCTGGAGGT GGTGGACCTG GAGGACGGGA AGGACAGAGA CCTCCATGTG 
AGCATCAAGA ACGCCTTCCG GCTGCACCGT GGCGCCACAG GGGACAGCCA CCTGCTGTGC 
ACCAGGAAGC CCGAGCAGAA GCAGCGCTGG CTCAAGGCCT TTGCCAGGGA GAGGGAGCAG 
GTGCAGCTGG ACCAGGAGAC AGGCTTCTCC ATCACTGAAC TGCAGAGGAA GCAGGCCATG 
CTGAATGCCA GCAAGCAGCA GGTCACAGGG AAGCCCAAAG CTGTTGGCCG GCCCTGCTAC 
CTGACGCGCC AGAAGCACCC AGCCCTGCCC AGCAACCGGC CCCAGCAGCA GGTCCTGGTG 
CTGGCGGAGC CCAGGCGCAA GCCATCTACC TTCTGGCACA GCATCAGCCG GCTGGCACCC 
TTCCGCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF4 ( XM_011511276.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509578.1 Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X12, mRNA.

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CloneID	OHu32422
Clone ID Related Accession (Same CDS sequence)	XM_005263688.3 , XM_005263689.4 , XM_011511277.2 , XM_011511277.1 , XM_005263689.5 , XM_024452938.1 , XM_011511276.2 , XM_005263688.2 , XM_011511276.3
Accession Version	XM_011511276.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011511276.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGACC AGGAGCTGGG CTTCAAAGCT GGGGACGTCA TCGAAGTGAT GGATGCCACC 
AACAGAGAGT GGTGGTGGGG CCGGGTCGCC GATGGCGAGG GCTGGTTTCC AGCCAGCTTC 
GTTCGGCTGA GGGTGAATCA GGACGAGCCC GCGGATGACG ACGCCCCTCT GGCCGGGAAC 
AGCGGAGCGG AGGACGGCGG GGCGGAGGCG CAGAGCAGCA AGGACCAGAT GCGGACCAAC 
GTCATCAACG AGATCCTCAG CACTGAGCGG GACTACATCA AGCACCTGCG CGACATCTGC 
GAGGGCTACG TCCGGCAGTG CCGCAAGCGC GCAGACATGT TCAGCGAGGA GCAGCTGCGT 
ACCATCTTCG GGAACATCGA GGACATCTAC CGCTGCCAGA AGGCCTTCGT GAAGGCCCTG 
GAGCAGAGGT TCAACCGCGA GCGCCCACAC CTGAGCGAGC TGGGTGCCTG CTTCCTGGAG 
CATCAAGCCG ACTTCCAGAT CTACTCGGAG TACTGCAATA ACCACCCCAA CGCCTGCGTG 
GAGCTCTCCC GGCTCACCAA GCTCAGCAAG TACGTGTACT TCTTCGAGGC CTGCCGGCTG 
CTGCAGAAGA TGATTGACAT CTCCCTGGAT GGCTTCCTGC TGACTCCGGT GCAGAAGATC 
TGCAAGTACC CTCTGCAGCT GGCCGAGCTG CTCAAATACA CGCACCCCCA GCACAGGGAC 
TTCAAGGATG TTGAAGCCGC CTTGCATGCC ATGAAGAACG TGGCCCAGCT CATCAACGAG 
CGGAAGCGGA GACTTGAGAA CATCGACAAG ATTGCTCAGT GGCAGAGCTC CATAGAGGAC 
TGGGAGGGAG AAGATCTCTT GGTCAGGAGC TCAGAACTCA TCTACTCGGG GGAGCTGACT 
CGAGTTACAC AGCCTCAAGC CAAAAGCCAG CAGCGAATGT TCTTTCTCTT TGACCACCAG 
CTCATCTACT GTAAGAAGGA CCTGCTCCGC CGCGACGTGT TGTACTACAA GGGCCGGCTG 
GACATGGACG GCCTGGAGGT GGTGGACCTG GAGGACGGGA AGGACAGAGA CCTCCATGTG 
AGCATCAAGA ACGCCTTCCG GCTGCACCGT GGCGCCACAG GGGACAGCCA CCTGCTGTGC 
ACCAGGAAGC CCGAGCAGAA GCAGCGCTGG CTCAAGGCCT TTGCCAGGGA GAGGGAGCAG 
GTGCAGCTGG ACCAGGAGAC AGGCTTCTCC ATCACTGAAC TGCAGAGGAA GCAGGCCATG 
CTGAATGCCA GCAAGCAGCA GGTCACAGGG AAGCCCAAAG CTGTTGGCCG GCCCTGCTAC 
CTGACGCGCC AGAAGCACCC AGCCCTGCCC AGCAACCGGC CCCAGCAGCA GGTCCTGGTG 
CTGGCGGAGC CCAGGCGCAA GCCATCTACC TTCTGGCACA GCATCAGCCG GCTGGCACCC 
TTCCGCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509578.1
CDS	80..1531
Translation	MDDQELGFKAGDVIEVMDATNREWWWGRVADGEGWFPASFVRLRVNQDEPADDDAPLAGNSGAEDGGAEAQSSKDQMRTNVINEILSTERDYIKHLRDICEGYVRQCRKRADMFSEEQLRTIFGNIEDIYRCQKAFVKALEQRFNRERPHLSELGACFLEHQADFQIYSEYCNNHPNACVELSRLTKLSKYVYFFEACRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTHPQHRDFKDVEAALHAMKNVAQLINERKRRLENIDKIAQWQSSIEDWEGEDLLVRSSELIYSGELTRVTQPQAKSQQRMFFLFDHQLIYCKKDLLRRDVLYYKGRLDMDGLEVVDLEDGKDRDLHVSIKNAFRLHRGATGDSHLLCTRKPEQKQRWLKAFAREREQVQLDQETGFSITELQRKQAMLNASKQQVTGKPKAVGRPCYLTRQKHPALPSNRPQQQVLVLAEPRRKPSTFWHSISRLAPFRK

Target ORF information:

RefSeq Version	XM_011511276.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511276.3

ORF Insert Sequence:

ATGGACGACC AGGAGCTGGG CTTCAAAGCT GGGGACGTCA TCGAAGTGAT GGATGCCACC 
AACAGAGAGT GGTGGTGGGG CCGGGTCGCC GATGGCGAGG GCTGGTTTCC AGCCAGCTTC 
GTTCGGCTGA GGGTGAATCA GGACGAGCCC GCGGATGACG ACGCCCCTCT GGCCGGGAAC 
AGCGGAGCGG AGGACGGCGG GGCGGAGGCG CAGAGCAGCA AGGACCAGAT GCGGACCAAC 
GTCATCAACG AGATCCTCAG CACTGAGCGG GACTACATCA AGCACCTGCG CGACATCTGC 
GAGGGCTACG TCCGGCAGTG CCGCAAGCGC GCAGACATGT TCAGCGAGGA GCAGCTGCGT 
ACCATCTTCG GGAACATCGA GGACATCTAC CGCTGCCAGA AGGCCTTCGT GAAGGCCCTG 
GAGCAGAGGT TCAACCGCGA GCGCCCACAC CTGAGCGAGC TGGGTGCCTG CTTCCTGGAG 
CATCAAGCCG ACTTCCAGAT CTACTCGGAG TACTGCAATA ACCACCCCAA CGCCTGCGTG 
GAGCTCTCCC GGCTCACCAA GCTCAGCAAG TACGTGTACT TCTTCGAGGC CTGCCGGCTG 
CTGCAGAAGA TGATTGACAT CTCCCTGGAT GGCTTCCTGC TGACTCCGGT GCAGAAGATC 
TGCAAGTACC CTCTGCAGCT GGCCGAGCTG CTCAAATACA CGCACCCCCA GCACAGGGAC 
TTCAAGGATG TTGAAGCCGC CTTGCATGCC ATGAAGAACG TGGCCCAGCT CATCAACGAG 
CGGAAGCGGA GACTTGAGAA CATCGACAAG ATTGCTCAGT GGCAGAGCTC CATAGAGGAC 
TGGGAGGGAG AAGATCTCTT GGTCAGGAGC TCAGAACTCA TCTACTCGGG GGAGCTGACT 
CGAGTTACAC AGCCTCAAGC CAAAAGCCAG CAGCGAATGT TCTTTCTCTT TGACCACCAG 
CTCATCTACT GTAAGAAGGA CCTGCTCCGC CGCGACGTGT TGTACTACAA GGGCCGGCTG 
GACATGGACG GCCTGGAGGT GGTGGACCTG GAGGACGGGA AGGACAGAGA CCTCCATGTG 
AGCATCAAGA ACGCCTTCCG GCTGCACCGT GGCGCCACAG GGGACAGCCA CCTGCTGTGC 
ACCAGGAAGC CCGAGCAGAA GCAGCGCTGG CTCAAGGCCT TTGCCAGGGA GAGGGAGCAG 
GTGCAGCTGG ACCAGGAGAC AGGCTTCTCC ATCACTGAAC TGCAGAGGAA GCAGGCCATG 
CTGAATGCCA GCAAGCAGCA GGTCACAGGG AAGCCCAAAG CTGTTGGCCG GCCCTGCTAC 
CTGACGCGCC AGAAGCACCC AGCCCTGCCC AGCAACCGGC CCCAGCAGCA GGTCCTGGTG 
CTGGCGGAGC CCAGGCGCAA GCCATCTACC TTCTGGCACA GCATCAGCCG GCTGGCACCC 
TTCCGCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF4 ( XM_005263687.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005263744.1 Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X7, mRNA.

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CloneID	OHu36332
Clone ID Related Accession (Same CDS sequence)	XM_005263687.4 , NM_001375904.1 , XM_005263687.3
Accession Version	XM_005263687.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005263687.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGCGCGGCG CCGTGTCCCG CTCCTGGAGC CTGGAGAGCC TGCGCTCGGC CACCGCCGCC 
ATGCCTGATG GAGCTCTGGA CACAGCTGTC TGCGCTGACG AAGTGGGGAG CGAGGAGGAC 
CTGTATGATG ACCTGCACAG CTCCAGCCAC CACTACAGCC ACCCTGGAGG GGGTGGGGAG 
CAGCTGGCTA TCAATGAGCT CATCAGCGAT GGCAGTGTGG TCTGCGCTGA AGCACTCTGG 
GACCATGTCA CCATGGACGA CCAGGAGCTG GGCTTCAAAG CTGGGGACGT CATCGAAGTG 
ATGGATGCCA CCAACAGAGA GTGGTGGTGG GGCCGGGTCG CCGATGGCGA GGGCTGGTTT 
CCAGCCAGCT TCGTTCGGCT GAGGGTGAAT CAGGACGAGC CCGCGGATGA CGACGCCCCT 
CTGGCCGGGA ACAGCGGAGC GGAGGACGGC GGGGCGGAGG CGCAGAGCAG CAAGGACCAG 
ATGCGGACCA ACGTCATCAA CGAGATCCTC AGCACTGAGC GGGACTACAT CAAGCACCTG 
CGCGACATCT GCGAGGGCTA CGTCCGGCAG TGCCGCAAGC GCGCAGACAT GTTCAGCGAG 
GAGCAGCTGC GTACCATCTT CGGGAACATC GAGGACATCT ACCGCTGCCA GAAGGCCTTC 
GTGAAGGCCC TGGAGCAGAG GTTCAACCGC GAGCGCCCAC ACCTGAGCGA GCTGGGTGCC 
TGCTTCCTGG AGCATCAAGC CGACTTCCAG ATCTACTCGG AGTACTGCAA TAACCACCCC 
AACGCCTGCG TGGAGCTCTC CCGGCTCACC AAGCTCAGCA AGTACGTGTA CTTCTTCGAG 
GCCTGCCGGC TGCTGCAGAA GATGATTGAC ATCTCCCTGG ATGGCTTCCT GCTGACTCCG 
GTGCAGAAGA TCTGCAAGTA CCCTCTGCAG CTGGCCGAGC TGCTCAAATA CACGCACCCC 
CAGCACAGGG ACTTCAAGGA TGTTGAAGCC GCCTTGCATG CCATGAAGAA CGTGGCCCAG 
CTCATCAACG AGCGGAAGCG GAGACTTGAG AACATCGACA AGATTGCTCA GTGGCAGAGC 
TCCATAGAGG ACTGGGAGGG AGAAGATCTC TTGGTCAGGA GCTCAGAACT CATCTACTCG 
GGGGAGCTGA CTCGAGTTAC ACAGCCTCAA GCCAAAAGCC AGCAGCGAAT GTTCTTTCTC 
TTTGACCACC AGCTCATCTA CTGTAAGAAG GACCTGCTCC GCCGCGACGT GTTGTACTAC 
AAGGGCCGGC TGGACATGGA CGGCCTGGAG GTGGTGGACC TGGAGGACGG GAAGGACAGA 
GACCTCCATG TGAGCATCAA GAACGCCTTC CGGCTGCACC GTGGCGCCAC AGGGGACAGC 
CACCTGCTGT GCACCAGGAA GCCCGAGCAG AAGCAGCGCT GGCTCAAGGC CTTTGCCAGG 
GAGAGGGAGC AGGTGCAGCT GGACCAGGAG ACAGGCTTCT CCATCACTGA ACTGCAGAGG 
AAGCAGGCCA TGCTGAATGC CAGCAAGCAG CAGGTCACAG GGAAGCCCAA AGCTGTTGGC 
CGGCCCTGCT ACCTGACGCG CCAGAAGCAC CCAGCCCTGC CCAGCAACCG GCCCCAGCAG 
CAGGTCCTGG TGCTGGCGGA GCCCAGGCGC AAGCCATCTA CCTTCTGGCA CAGCATCAGC 
CGGCTGGCAC CCTTCCGCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005263744.1
CDS	180..1883
Translation	MRGAVSRSWSLESLRSATAAMPDGALDTAVCADEVGSEEDLYDDLHSSSHHYSHPGGGGEQLAINELISDGSVVCAEALWDHVTMDDQELGFKAGDVIEVMDATNREWWWGRVADGEGWFPASFVRLRVNQDEPADDDAPLAGNSGAEDGGAEAQSSKDQMRTNVINEILSTERDYIKHLRDICEGYVRQCRKRADMFSEEQLRTIFGNIEDIYRCQKAFVKALEQRFNRERPHLSELGACFLEHQADFQIYSEYCNNHPNACVELSRLTKLSKYVYFFEACRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTHPQHRDFKDVEAALHAMKNVAQLINERKRRLENIDKIAQWQSSIEDWEGEDLLVRSSELIYSGELTRVTQPQAKSQQRMFFLFDHQLIYCKKDLLRRDVLYYKGRLDMDGLEVVDLEDGKDRDLHVSIKNAFRLHRGATGDSHLLCTRKPEQKQRWLKAFAREREQVQLDQETGFSITELQRKQAMLNASKQQVTGKPKAVGRPCYLTRQKHPALPSNRPQQQVLVLAEPRRKPSTFWHSISRLAPFRK

Target ORF information:

RefSeq Version	XM_005263687.4
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005263687.4

ORF Insert Sequence:

ATGCGCGGCG CCGTGTCCCG CTCCTGGAGC CTGGAGAGCC TGCGCTCGGC CACCGCCGCC 
ATGCCTGATG GAGCTCTGGA CACAGCTGTC TGCGCTGACG AAGTGGGGAG CGAGGAGGAC 
CTGTATGATG ACCTGCACAG CTCCAGCCAC CACTACAGCC ACCCTGGAGG GGGTGGGGAG 
CAGCTGGCTA TCAATGAGCT CATCAGCGAT GGCAGTGTGG TCTGCGCTGA AGCACTCTGG 
GACCATGTCA CCATGGACGA CCAGGAGCTG GGCTTCAAAG CTGGGGACGT CATCGAAGTG 
ATGGATGCCA CCAACAGAGA GTGGTGGTGG GGCCGGGTCG CCGATGGCGA GGGCTGGTTT 
CCAGCCAGCT TCGTTCGGCT GAGGGTGAAT CAGGACGAGC CCGCGGATGA CGACGCCCCT 
CTGGCCGGGA ACAGCGGAGC GGAGGACGGC GGGGCGGAGG CGCAGAGCAG CAAGGACCAG 
ATGCGGACCA ACGTCATCAA CGAGATCCTC AGCACTGAGC GGGACTACAT CAAGCACCTG 
CGCGACATCT GCGAGGGCTA CGTCCGGCAG TGCCGCAAGC GCGCAGACAT GTTCAGCGAG 
GAGCAGCTGC GTACCATCTT CGGGAACATC GAGGACATCT ACCGCTGCCA GAAGGCCTTC 
GTGAAGGCCC TGGAGCAGAG GTTCAACCGC GAGCGCCCAC ACCTGAGCGA GCTGGGTGCC 
TGCTTCCTGG AGCATCAAGC CGACTTCCAG ATCTACTCGG AGTACTGCAA TAACCACCCC 
AACGCCTGCG TGGAGCTCTC CCGGCTCACC AAGCTCAGCA AGTACGTGTA CTTCTTCGAG 
GCCTGCCGGC TGCTGCAGAA GATGATTGAC ATCTCCCTGG ATGGCTTCCT GCTGACTCCG 
GTGCAGAAGA TCTGCAAGTA CCCTCTGCAG CTGGCCGAGC TGCTCAAATA CACGCACCCC 
CAGCACAGGG ACTTCAAGGA TGTTGAAGCC GCCTTGCATG CCATGAAGAA CGTGGCCCAG 
CTCATCAACG AGCGGAAGCG GAGACTTGAG AACATCGACA AGATTGCTCA GTGGCAGAGC 
TCCATAGAGG ACTGGGAGGG AGAAGATCTC TTGGTCAGGA GCTCAGAACT CATCTACTCG 
GGGGAGCTGA CTCGAGTTAC ACAGCCTCAA GCCAAAAGCC AGCAGCGAAT GTTCTTTCTC 
TTTGACCACC AGCTCATCTA CTGTAAGAAG GACCTGCTCC GCCGCGACGT GTTGTACTAC 
AAGGGCCGGC TGGACATGGA CGGCCTGGAG GTGGTGGACC TGGAGGACGG GAAGGACAGA 
GACCTCCATG TGAGCATCAA GAACGCCTTC CGGCTGCACC GTGGCGCCAC AGGGGACAGC 
CACCTGCTGT GCACCAGGAA GCCCGAGCAG AAGCAGCGCT GGCTCAAGGC CTTTGCCAGG 
GAGAGGGAGC AGGTGCAGCT GGACCAGGAG ACAGGCTTCT CCATCACTGA ACTGCAGAGG 
AAGCAGGCCA TGCTGAATGC CAGCAAGCAG CAGGTCACAG GGAAGCCCAA AGCTGTTGGC 
CGGCCCTGCT ACCTGACGCG CCAGAAGCAC CCAGCCCTGC CCAGCAACCG GCCCCAGCAG 
CAGGTCCTGG TGCTGGCGGA GCCCAGGCGC AAGCCATCTA CCTTCTGGCA CAGCATCAGC 
CGGCTGGCAC CCTTCCGCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF4 ( XM_005263687.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005263744.1 Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu36332
Clone ID Related Accession (Same CDS sequence)	XM_005263687.4 , NM_001375904.1 , XM_005263687.3
Accession Version	XM_005263687.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005263687.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGGCG CCGTGTCCCG CTCCTGGAGC CTGGAGAGCC TGCGCTCGGC CACCGCCGCC 
ATGCCTGATG GAGCTCTGGA CACAGCTGTC TGCGCTGACG AAGTGGGGAG CGAGGAGGAC 
CTGTATGATG ACCTGCACAG CTCCAGCCAC CACTACAGCC ACCCTGGAGG GGGTGGGGAG 
CAGCTGGCTA TCAATGAGCT CATCAGCGAT GGCAGTGTGG TCTGCGCTGA AGCACTCTGG 
GACCATGTCA CCATGGACGA CCAGGAGCTG GGCTTCAAAG CTGGGGACGT CATCGAAGTG 
ATGGATGCCA CCAACAGAGA GTGGTGGTGG GGCCGGGTCG CCGATGGCGA GGGCTGGTTT 
CCAGCCAGCT TCGTTCGGCT GAGGGTGAAT CAGGACGAGC CCGCGGATGA CGACGCCCCT 
CTGGCCGGGA ACAGCGGAGC GGAGGACGGC GGGGCGGAGG CGCAGAGCAG CAAGGACCAG 
ATGCGGACCA ACGTCATCAA CGAGATCCTC AGCACTGAGC GGGACTACAT CAAGCACCTG 
CGCGACATCT GCGAGGGCTA CGTCCGGCAG TGCCGCAAGC GCGCAGACAT GTTCAGCGAG 
GAGCAGCTGC GTACCATCTT CGGGAACATC GAGGACATCT ACCGCTGCCA GAAGGCCTTC 
GTGAAGGCCC TGGAGCAGAG GTTCAACCGC GAGCGCCCAC ACCTGAGCGA GCTGGGTGCC 
TGCTTCCTGG AGCATCAAGC CGACTTCCAG ATCTACTCGG AGTACTGCAA TAACCACCCC 
AACGCCTGCG TGGAGCTCTC CCGGCTCACC AAGCTCAGCA AGTACGTGTA CTTCTTCGAG 
GCCTGCCGGC TGCTGCAGAA GATGATTGAC ATCTCCCTGG ATGGCTTCCT GCTGACTCCG 
GTGCAGAAGA TCTGCAAGTA CCCTCTGCAG CTGGCCGAGC TGCTCAAATA CACGCACCCC 
CAGCACAGGG ACTTCAAGGA TGTTGAAGCC GCCTTGCATG CCATGAAGAA CGTGGCCCAG 
CTCATCAACG AGCGGAAGCG GAGACTTGAG AACATCGACA AGATTGCTCA GTGGCAGAGC 
TCCATAGAGG ACTGGGAGGG AGAAGATCTC TTGGTCAGGA GCTCAGAACT CATCTACTCG 
GGGGAGCTGA CTCGAGTTAC ACAGCCTCAA GCCAAAAGCC AGCAGCGAAT GTTCTTTCTC 
TTTGACCACC AGCTCATCTA CTGTAAGAAG GACCTGCTCC GCCGCGACGT GTTGTACTAC 
AAGGGCCGGC TGGACATGGA CGGCCTGGAG GTGGTGGACC TGGAGGACGG GAAGGACAGA 
GACCTCCATG TGAGCATCAA GAACGCCTTC CGGCTGCACC GTGGCGCCAC AGGGGACAGC 
CACCTGCTGT GCACCAGGAA GCCCGAGCAG AAGCAGCGCT GGCTCAAGGC CTTTGCCAGG 
GAGAGGGAGC AGGTGCAGCT GGACCAGGAG ACAGGCTTCT CCATCACTGA ACTGCAGAGG 
AAGCAGGCCA TGCTGAATGC CAGCAAGCAG CAGGTCACAG GGAAGCCCAA AGCTGTTGGC 
CGGCCCTGCT ACCTGACGCG CCAGAAGCAC CCAGCCCTGC CCAGCAACCG GCCCCAGCAG 
CAGGTCCTGG TGCTGGCGGA GCCCAGGCGC AAGCCATCTA CCTTCTGGCA CAGCATCAGC 
CGGCTGGCAC CCTTCCGCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005263744.1
CDS	180..1883
Translation	MRGAVSRSWSLESLRSATAAMPDGALDTAVCADEVGSEEDLYDDLHSSSHHYSHPGGGGEQLAINELISDGSVVCAEALWDHVTMDDQELGFKAGDVIEVMDATNREWWWGRVADGEGWFPASFVRLRVNQDEPADDDAPLAGNSGAEDGGAEAQSSKDQMRTNVINEILSTERDYIKHLRDICEGYVRQCRKRADMFSEEQLRTIFGNIEDIYRCQKAFVKALEQRFNRERPHLSELGACFLEHQADFQIYSEYCNNHPNACVELSRLTKLSKYVYFFEACRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTHPQHRDFKDVEAALHAMKNVAQLINERKRRLENIDKIAQWQSSIEDWEGEDLLVRSSELIYSGELTRVTQPQAKSQQRMFFLFDHQLIYCKKDLLRRDVLYYKGRLDMDGLEVVDLEDGKDRDLHVSIKNAFRLHRGATGDSHLLCTRKPEQKQRWLKAFAREREQVQLDQETGFSITELQRKQAMLNASKQQVTGKPKAVGRPCYLTRQKHPALPSNRPQQQVLVLAEPRRKPSTFWHSISRLAPFRK

Target ORF information:

RefSeq Version	XM_005263687.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005263687.3

ORF Insert Sequence:

ATGCGCGGCG CCGTGTCCCG CTCCTGGAGC CTGGAGAGCC TGCGCTCGGC CACCGCCGCC 
ATGCCTGATG GAGCTCTGGA CACAGCTGTC TGCGCTGACG AAGTGGGGAG CGAGGAGGAC 
CTGTATGATG ACCTGCACAG CTCCAGCCAC CACTACAGCC ACCCTGGAGG GGGTGGGGAG 
CAGCTGGCTA TCAATGAGCT CATCAGCGAT GGCAGTGTGG TCTGCGCTGA AGCACTCTGG 
GACCATGTCA CCATGGACGA CCAGGAGCTG GGCTTCAAAG CTGGGGACGT CATCGAAGTG 
ATGGATGCCA CCAACAGAGA GTGGTGGTGG GGCCGGGTCG CCGATGGCGA GGGCTGGTTT 
CCAGCCAGCT TCGTTCGGCT GAGGGTGAAT CAGGACGAGC CCGCGGATGA CGACGCCCCT 
CTGGCCGGGA ACAGCGGAGC GGAGGACGGC GGGGCGGAGG CGCAGAGCAG CAAGGACCAG 
ATGCGGACCA ACGTCATCAA CGAGATCCTC AGCACTGAGC GGGACTACAT CAAGCACCTG 
CGCGACATCT GCGAGGGCTA CGTCCGGCAG TGCCGCAAGC GCGCAGACAT GTTCAGCGAG 
GAGCAGCTGC GTACCATCTT CGGGAACATC GAGGACATCT ACCGCTGCCA GAAGGCCTTC 
GTGAAGGCCC TGGAGCAGAG GTTCAACCGC GAGCGCCCAC ACCTGAGCGA GCTGGGTGCC 
TGCTTCCTGG AGCATCAAGC CGACTTCCAG ATCTACTCGG AGTACTGCAA TAACCACCCC 
AACGCCTGCG TGGAGCTCTC CCGGCTCACC AAGCTCAGCA AGTACGTGTA CTTCTTCGAG 
GCCTGCCGGC TGCTGCAGAA GATGATTGAC ATCTCCCTGG ATGGCTTCCT GCTGACTCCG 
GTGCAGAAGA TCTGCAAGTA CCCTCTGCAG CTGGCCGAGC TGCTCAAATA CACGCACCCC 
CAGCACAGGG ACTTCAAGGA TGTTGAAGCC GCCTTGCATG CCATGAAGAA CGTGGCCCAG 
CTCATCAACG AGCGGAAGCG GAGACTTGAG AACATCGACA AGATTGCTCA GTGGCAGAGC 
TCCATAGAGG ACTGGGAGGG AGAAGATCTC TTGGTCAGGA GCTCAGAACT CATCTACTCG 
GGGGAGCTGA CTCGAGTTAC ACAGCCTCAA GCCAAAAGCC AGCAGCGAAT GTTCTTTCTC 
TTTGACCACC AGCTCATCTA CTGTAAGAAG GACCTGCTCC GCCGCGACGT GTTGTACTAC 
AAGGGCCGGC TGGACATGGA CGGCCTGGAG GTGGTGGACC TGGAGGACGG GAAGGACAGA 
GACCTCCATG TGAGCATCAA GAACGCCTTC CGGCTGCACC GTGGCGCCAC AGGGGACAGC 
CACCTGCTGT GCACCAGGAA GCCCGAGCAG AAGCAGCGCT GGCTCAAGGC CTTTGCCAGG 
GAGAGGGAGC AGGTGCAGCT GGACCAGGAG ACAGGCTTCT CCATCACTGA ACTGCAGAGG 
AAGCAGGCCA TGCTGAATGC CAGCAAGCAG CAGGTCACAG GGAAGCCCAA AGCTGTTGGC 
CGGCCCTGCT ACCTGACGCG CCAGAAGCAC CCAGCCCTGC CCAGCAACCG GCCCCAGCAG 
CAGGTCCTGG TGCTGGCGGA GCCCAGGCGC AAGCCATCTA CCTTCTGGCA CAGCATCAGC 
CGGCTGGCAC CCTTCCGCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF4 ( NM_001375900.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001362829.1 Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant 4, mRNA.

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CloneID	OHu109041
Clone ID Related Accession (Same CDS sequence)	NM_001375900.1
Accession Version	NM_001375900.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-05
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 4 isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC133785.2, AC112786.4 and AC009477.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.95222.1, SRR1660803.211267.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCTCAGCG TCGTGCACTT CCTCCGGAGC TTCTTCAAGA ACCACATGCC CTGGGAAGAA 
CCAGCAGGTG AGAAGCCCAG TTGCTCTCAC AGTCAGAAGG CGTTCCACAT GGAGCCTGCC 
CAGAAGCCCT GCTTCACCAC TGACATGGTG ACATGGGCCC TCCTCTGCAT CTCTGCAGAG 
ACTGTGCGTG GGGAGGCTCC TTCACAGCCT AGGGGCATCC CTCACCGCTC GCCCGTCAGT 
GTGGATGACC TGTGGCTGGA GAAGACACAG AGAAAGAAGT TGCAGAAGCA GGCCCACGTC 
GAAAGGAGGC TGCACATAGG GGCAGTGCAC AAAGATGGAG TCAAGTGCTG GAGAAAGACG 
ATCATTACCT CTCCAGAGTC TTTGAATCTC CCTAGAAGAA GCCATCCACT CTCCCAGAGT 
GCTCCAACGG GACTGAACCA CATGGGCTGG CCAGAGCACA CACCAGGCAC TGCCATGCCT 
GATGGAGCTC TGGACACAGC TGTCTGCGCT GACGAAGTGG GGAGCGAGGA GGACCTGTAT 
GATGACCTGC ACAGCTCCAG CCACCACTAC AGCCACCCTG GAGGGGGTGG GGAGCAGCTG 
GCTATCAATG AGCTCATCAG CGATGGCAGT GTGGTCTGCG CTGAAGCACT CTGGGACCAT 
GTCACCATGG ACGACCAGGA GCTGGGCTTC AAAGCTGGGG ACGTCATCGA AGTGATGGAT 
GCCACCAACA GAGAGTGGTG GTGGGGCCGG GTCGCCGATG GCGAGGGCTG GTTTCCAGCC 
AGCTTCGTTC GGCTGAGGGT GAATCAGGAC GAGCCCGCGG ATGACGACGC CCCTCTGGCC 
GGGAACAGCG GAGCGGAGGA CGGCGGGGCG GAGGCGCAGA GCAGCAAGGA CCAGATGCGG 
ACCAACGTCA TCAACGAGAT CCTCAGCACT GAGCGGGACT ACATCAAGCA CCTGCGCGAC 
ATCTGCGAGG GCTACGTCCG GCAGTGCCGC AAGCGCGCAG ACATGTTCAG CGAGGAGCAG 
CTGCGTACCA TCTTCGGGAA CATCGAGGAC ATCTACCGCT GCCAGAAGGC CTTCGTGAAG 
GCCCTGGAGC AGAGGTTCAA CCGCGAGCGC CCACACCTGA GCGAGCTGGG TGCCTGCTTC 
CTGGAGCATC AAGCCGACTT CCAGATCTAC TCGGAGTACT GCAATAACCA CCCCAACGCC 
TGCGTGGAGC TCTCCCGGCT CACCAAGCTC AGCAAGTACG TGTACTTCTT CGAGGCCTGC 
CGGCTGCTGC AGAAGATGAT TGACATCTCC CTGGATGGCT TCCTGCTGAC TCCGGTGCAG 
AAGATCTGCA AGTACCCTCT GCAGCTGGCC GAGCTGCTCA AATACACGCA CCCCCAGCAC 
AGGGACTTCA AGGATGTTGA AGCCGCCTTG CATGCCATGA AGAACGTGGC CCAGCTCATC 
AACGAGCGGA AGCGGAGACT TGAGAACATC GACAAGATTG CTCAGTGGCA GAGCTCCATA 
GAGGACTGGG AGGGAGAAGA TCTCTTGGTC AGGAGCTCAG AACTCATCTA CTCGGGGGAG 
CTGACTCGAG TTACACAGCC TCAAGCCAAA AGCCAGCAGC GAATGTTCTT TCTCTTTGAC 
CACCAGCTCA TCTACTGTAA GAAGGACCTG CTCCGCCGCG ACGTGTTGTA CTACAAGGGC 
CGGCTGGACA TGGACGGCCT GGAGGTGGTG GACCTGGAGG ACGGGAAGGA CAGAGACCTC 
CATGTGAGCA TCAAGAACGC CTTCCGGCTG CACCGTGGCG CCACAGGGGA CAGCCACCTG 
CTGTGCACCA GGAAGCCCGA GCAGAAGCAG CGCTGGCTCA AGGCCTTTGC CAGGGAGAGG 
GAGCAGGTGC AGCTGGACCA GGAGACAGGC TTCTCCATCA CTGAACTGCA GAGGAAGCAG 
GCCATGCTGA ATGCCAGCAA GCAGCAGGTC ACAGGGAAGC CCAAAGCTGT TGGCCGGCCC 
TGCTACCTGA CGCGCCAGAA GCACCCAGCC CTGCCCAGCA ACCGGCCCCA GCAGCAGGTC 
CTGGTGCTGG CGGAGCCCAG GCGCAAGCCA TCTACCTTCT GGCACAGCAT CAGCCGGCTG 
GCACCCTTCC GCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001362829.1
CDS	41..2158
Translation	MLSVVHFLRSFFKNHMPWEEPAGEKPSCSHSQKAFHMEPAQKPCFTTDMVTWALLCISAETVRGEAPSQPRGIPHRSPVSVDDLWLEKTQRKKLQKQAHVERRLHIGAVHKDGVKCWRKTIITSPESLNLPRRSHPLSQSAPTGLNHMGWPEHTPGTAMPDGALDTAVCADEVGSEEDLYDDLHSSSHHYSHPGGGGEQLAINELISDGSVVCAEALWDHVTMDDQELGFKAGDVIEVMDATNREWWWGRVADGEGWFPASFVRLRVNQDEPADDDAPLAGNSGAEDGGAEAQSSKDQMRTNVINEILSTERDYIKHLRDICEGYVRQCRKRADMFSEEQLRTIFGNIEDIYRCQKAFVKALEQRFNRERPHLSELGACFLEHQADFQIYSEYCNNHPNACVELSRLTKLSKYVYFFEACRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTHPQHRDFKDVEAALHAMKNVAQLINERKRRLENIDKIAQWQSSIEDWEGEDLLVRSSELIYSGELTRVTQPQAKSQQRMFFLFDHQLIYCKKDLLRRDVLYYKGRLDMDGLEVVDLEDGKDRDLHVSIKNAFRLHRGATGDSHLLCTRKPEQKQRWLKAFAREREQVQLDQETGFSITELQRKQAMLNASKQQVTGKPKAVGRPCYLTRQKHPALPSNRPQQQVLVLAEPRRKPSTFWHSISRLAPFRK

Target ORF information:

RefSeq Version	NM_001375900.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001375900.1

ORF Insert Sequence:

ATGCTCAGCG TCGTGCACTT CCTCCGGAGC TTCTTCAAGA ACCACATGCC CTGGGAAGAA 
CCAGCAGGTG AGAAGCCCAG TTGCTCTCAC AGTCAGAAGG CGTTCCACAT GGAGCCTGCC 
CAGAAGCCCT GCTTCACCAC TGACATGGTG ACATGGGCCC TCCTCTGCAT CTCTGCAGAG 
ACTGTGCGTG GGGAGGCTCC TTCACAGCCT AGGGGCATCC CTCACCGCTC GCCCGTCAGT 
GTGGATGACC TGTGGCTGGA GAAGACACAG AGAAAGAAGT TGCAGAAGCA GGCCCACGTC 
GAAAGGAGGC TGCACATAGG GGCAGTGCAC AAAGATGGAG TCAAGTGCTG GAGAAAGACG 
ATCATTACCT CTCCAGAGTC TTTGAATCTC CCTAGAAGAA GCCATCCACT CTCCCAGAGT 
GCTCCAACGG GACTGAACCA CATGGGCTGG CCAGAGCACA CACCAGGCAC TGCCATGCCT 
GATGGAGCTC TGGACACAGC TGTCTGCGCT GACGAAGTGG GGAGCGAGGA GGACCTGTAT 
GATGACCTGC ACAGCTCCAG CCACCACTAC AGCCACCCTG GAGGGGGTGG GGAGCAGCTG 
GCTATCAATG AGCTCATCAG CGATGGCAGT GTGGTCTGCG CTGAAGCACT CTGGGACCAT 
GTCACCATGG ACGACCAGGA GCTGGGCTTC AAAGCTGGGG ACGTCATCGA AGTGATGGAT 
GCCACCAACA GAGAGTGGTG GTGGGGCCGG GTCGCCGATG GCGAGGGCTG GTTTCCAGCC 
AGCTTCGTTC GGCTGAGGGT GAATCAGGAC GAGCCCGCGG ATGACGACGC CCCTCTGGCC 
GGGAACAGCG GAGCGGAGGA CGGCGGGGCG GAGGCGCAGA GCAGCAAGGA CCAGATGCGG 
ACCAACGTCA TCAACGAGAT CCTCAGCACT GAGCGGGACT ACATCAAGCA CCTGCGCGAC 
ATCTGCGAGG GCTACGTCCG GCAGTGCCGC AAGCGCGCAG ACATGTTCAG CGAGGAGCAG 
CTGCGTACCA TCTTCGGGAA CATCGAGGAC ATCTACCGCT GCCAGAAGGC CTTCGTGAAG 
GCCCTGGAGC AGAGGTTCAA CCGCGAGCGC CCACACCTGA GCGAGCTGGG TGCCTGCTTC 
CTGGAGCATC AAGCCGACTT CCAGATCTAC TCGGAGTACT GCAATAACCA CCCCAACGCC 
TGCGTGGAGC TCTCCCGGCT CACCAAGCTC AGCAAGTACG TGTACTTCTT CGAGGCCTGC 
CGGCTGCTGC AGAAGATGAT TGACATCTCC CTGGATGGCT TCCTGCTGAC TCCGGTGCAG 
AAGATCTGCA AGTACCCTCT GCAGCTGGCC GAGCTGCTCA AATACACGCA CCCCCAGCAC 
AGGGACTTCA AGGATGTTGA AGCCGCCTTG CATGCCATGA AGAACGTGGC CCAGCTCATC 
AACGAGCGGA AGCGGAGACT TGAGAACATC GACAAGATTG CTCAGTGGCA GAGCTCCATA 
GAGGACTGGG AGGGAGAAGA TCTCTTGGTC AGGAGCTCAG AACTCATCTA CTCGGGGGAG 
CTGACTCGAG TTACACAGCC TCAAGCCAAA AGCCAGCAGC GAATGTTCTT TCTCTTTGAC 
CACCAGCTCA TCTACTGTAA GAAGGACCTG CTCCGCCGCG ACGTGTTGTA CTACAAGGGC 
CGGCTGGACA TGGACGGCCT GGAGGTGGTG GACCTGGAGG ACGGGAAGGA CAGAGACCTC 
CATGTGAGCA TCAAGAACGC CTTCCGGCTG CACCGTGGCG CCACAGGGGA CAGCCACCTG 
CTGTGCACCA GGAAGCCCGA GCAGAAGCAG CGCTGGCTCA AGGCCTTTGC CAGGGAGAGG 
GAGCAGGTGC AGCTGGACCA GGAGACAGGC TTCTCCATCA CTGAACTGCA GAGGAAGCAG 
GCCATGCTGA ATGCCAGCAA GCAGCAGGTC ACAGGGAAGC CCAAAGCTGT TGGCCGGCCC 
TGCTACCTGA CGCGCCAGAA GCACCCAGCC CTGCCCAGCA ACCGGCCCCA GCAGCAGGTC 
CTGGTGCTGG CGGAGCCCAG GCGCAAGCCA TCTACCTTCT GGCACAGCAT CAGCCGGCTG 
GCACCCTTCC GCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF4 ( NM_001375901.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001362830.1 Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant 5, mRNA.

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CloneID	OHu109043
Clone ID Related Accession (Same CDS sequence)	NM_001375901.1
Accession Version	NM_001375901.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-05
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 4 isoform e
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC133785.2, AC112786.4 and AC009477.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.118599.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCCCTGGG AAGAACCAGC AGAGACTGTG CGTGGGGAGG CTCCTTCACA GCCTAGGGGC 
ATCCCTCACC GCTCGCCCGT CAGTGTGGAT GACCTGTGGC TGGAGAAGAC ACAGAGAAAG 
AAGTTGCAGA AGCAGGCCCA CGTCGAAAGG AGGCTGCACA TAGGGGCAGT GCACAAAGAT 
GGAGTCAAGT GCTGGAGAAA GACGATCATT ACCTCTCCAG AGTCTTTGAA TCTCCCTAGA 
AGAAGCCATC CACTCTCCCA GAGTGCTCCA ACGGGACTGA ACCACATGGG CTGGCCAGAG 
CACACACCAG GCACTGCCAT GCCTGATGGA GCTCTGGACA CAGCTGTCTG CGCTGACGAA 
GTGGGGAGCG AGGAGGACCT GTATGATGAC CTGCACAGCT CCAGCCACCA CTACAGCCAC 
CCTGGAGGGG GTGGGGAGCA GCTGGCTATC AATGAGCTCA TCAGCGATGG CAGTGTGGTC 
TGCGCTGAAG CACTCTGGGA CCATGTCACC ATGGACGACC AGGAGCTGGG CTTCAAAGCT 
GGGGACGTCA TCGAAGTGAT GGATGCCACC AACAGAGAGT GGTGGTGGGG CCGGGTCGCC 
GATGGCGAGG GCTGGTTTCC AGCCAGCTTC GTTCGGCTGA GGGTGAATCA GGACGAGCCC 
GCGGATGACG ACGCCCCTCT GGCCGGGAAC AGCGGAGCGG AGGACGGCGG GGCGGAGGCG 
CAGAGCAGCA AGGACCAGAT GCGGACCAAC GTCATCAACG AGATCCTCAG CACTGAGCGG 
GACTACATCA AGCACCTGCG CGACATCTGC GAGGGCTACG TCCGGCAGTG CCGCAAGCGC 
GCAGACATGT TCAGCGAGGA GCAGCTGCGT ACCATCTTCG GGAACATCGA GGACATCTAC 
CGCTGCCAGA AGGCCTTCGT GAAGGCCCTG GAGCAGAGGT TCAACCGCGA GCGCCCACAC 
CTGAGCGAGC TGGGTGCCTG CTTCCTGGAG CATCAAGCCG ACTTCCAGAT CTACTCGGAG 
TACTGCAATA ACCACCCCAA CGCCTGCGTG GAGCTCTCCC GGCTCACCAA GCTCAGCAAG 
TACGTGTACT TCTTCGAGGC CTGCCGGCTG CTGCAGAAGA TGATTGACAT CTCCCTGGAT 
GGCTTCCTGC TGACTCCGGT GCAGAAGATC TGCAAGTACC CTCTGCAGCT GGCCGAGCTG 
CTCAAATACA CGCACCCCCA GCACAGGGAC TTCAAGGATG TTGAAGCCGC CTTGCATGCC 
ATGAAGAACG TGGCCCAGCT CATCAACGAG CGGAAGCGGA GACTTGAGAA CATCGACAAG 
ATTGCTCAGT GGCAGAGCTC CATAGAGGAC TGGGAGGGAG AAGATCTCTT GGTCAGGAGC 
TCAGAACTCA TCTACTCGGG GGAGCTGACT CGAGTTACAC AGCCTCAAGC CAAAAGCCAG 
CAGCGAATGT TCTTTCTCTT TGACCACCAG CTCATCTACT GTAAGAAGGA CCTGCTCCGC 
CGCGACGTGT TGTACTACAA GGGCCGGCTG GACATGGACG GCCTGGAGGT GGTGGACCTG 
GAGGACGGGA AGGACAGAGA CCTCCATGTG AGCATCAAGA ACGCCTTCCG GCTGCACCGT 
GGCGCCACAG GGGACAGCCA CCTGCTGTGC ACCAGGAAGC CCGAGCAGAA GCAGCGCTGG 
CTCAAGGCCT TTGCCAGGGA GAGGGAGCAG GTGCAGCTGG ACCAGGAGAC AGGCTTCTCC 
ATCACTGAAC TGCAGAGGAA GCAGGCCATG CTGAATGCCA GCAAGCAGCA GGTCACAGGG 
AAGCCCAAAG CTGTTGGCCG GCCCTGCTAC CTGACGCGCC AGAAGCACCC AGCCCTGCCC 
AGCAACCGGC CCCAGCAGCA GGTCCTGGTG CTGGCGGAGC CCAGGCGCAA GCCATCTACC 
TTCTGGCACA GCATCAGCCG GCTGGCACCC TTCCGCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001362830.1
CDS	222..2183
Translation	MPWEEPAETVRGEAPSQPRGIPHRSPVSVDDLWLEKTQRKKLQKQAHVERRLHIGAVHKDGVKCWRKTIITSPESLNLPRRSHPLSQSAPTGLNHMGWPEHTPGTAMPDGALDTAVCADEVGSEEDLYDDLHSSSHHYSHPGGGGEQLAINELISDGSVVCAEALWDHVTMDDQELGFKAGDVIEVMDATNREWWWGRVADGEGWFPASFVRLRVNQDEPADDDAPLAGNSGAEDGGAEAQSSKDQMRTNVINEILSTERDYIKHLRDICEGYVRQCRKRADMFSEEQLRTIFGNIEDIYRCQKAFVKALEQRFNRERPHLSELGACFLEHQADFQIYSEYCNNHPNACVELSRLTKLSKYVYFFEACRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTHPQHRDFKDVEAALHAMKNVAQLINERKRRLENIDKIAQWQSSIEDWEGEDLLVRSSELIYSGELTRVTQPQAKSQQRMFFLFDHQLIYCKKDLLRRDVLYYKGRLDMDGLEVVDLEDGKDRDLHVSIKNAFRLHRGATGDSHLLCTRKPEQKQRWLKAFAREREQVQLDQETGFSITELQRKQAMLNASKQQVTGKPKAVGRPCYLTRQKHPALPSNRPQQQVLVLAEPRRKPSTFWHSISRLAPFRK

Target ORF information:

RefSeq Version	NM_001375901.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001375901.1

ORF Insert Sequence:

ATGCCCTGGG AAGAACCAGC AGAGACTGTG CGTGGGGAGG CTCCTTCACA GCCTAGGGGC 
ATCCCTCACC GCTCGCCCGT CAGTGTGGAT GACCTGTGGC TGGAGAAGAC ACAGAGAAAG 
AAGTTGCAGA AGCAGGCCCA CGTCGAAAGG AGGCTGCACA TAGGGGCAGT GCACAAAGAT 
GGAGTCAAGT GCTGGAGAAA GACGATCATT ACCTCTCCAG AGTCTTTGAA TCTCCCTAGA 
AGAAGCCATC CACTCTCCCA GAGTGCTCCA ACGGGACTGA ACCACATGGG CTGGCCAGAG 
CACACACCAG GCACTGCCAT GCCTGATGGA GCTCTGGACA CAGCTGTCTG CGCTGACGAA 
GTGGGGAGCG AGGAGGACCT GTATGATGAC CTGCACAGCT CCAGCCACCA CTACAGCCAC 
CCTGGAGGGG GTGGGGAGCA GCTGGCTATC AATGAGCTCA TCAGCGATGG CAGTGTGGTC 
TGCGCTGAAG CACTCTGGGA CCATGTCACC ATGGACGACC AGGAGCTGGG CTTCAAAGCT 
GGGGACGTCA TCGAAGTGAT GGATGCCACC AACAGAGAGT GGTGGTGGGG CCGGGTCGCC 
GATGGCGAGG GCTGGTTTCC AGCCAGCTTC GTTCGGCTGA GGGTGAATCA GGACGAGCCC 
GCGGATGACG ACGCCCCTCT GGCCGGGAAC AGCGGAGCGG AGGACGGCGG GGCGGAGGCG 
CAGAGCAGCA AGGACCAGAT GCGGACCAAC GTCATCAACG AGATCCTCAG CACTGAGCGG 
GACTACATCA AGCACCTGCG CGACATCTGC GAGGGCTACG TCCGGCAGTG CCGCAAGCGC 
GCAGACATGT TCAGCGAGGA GCAGCTGCGT ACCATCTTCG GGAACATCGA GGACATCTAC 
CGCTGCCAGA AGGCCTTCGT GAAGGCCCTG GAGCAGAGGT TCAACCGCGA GCGCCCACAC 
CTGAGCGAGC TGGGTGCCTG CTTCCTGGAG CATCAAGCCG ACTTCCAGAT CTACTCGGAG 
TACTGCAATA ACCACCCCAA CGCCTGCGTG GAGCTCTCCC GGCTCACCAA GCTCAGCAAG 
TACGTGTACT TCTTCGAGGC CTGCCGGCTG CTGCAGAAGA TGATTGACAT CTCCCTGGAT 
GGCTTCCTGC TGACTCCGGT GCAGAAGATC TGCAAGTACC CTCTGCAGCT GGCCGAGCTG 
CTCAAATACA CGCACCCCCA GCACAGGGAC TTCAAGGATG TTGAAGCCGC CTTGCATGCC 
ATGAAGAACG TGGCCCAGCT CATCAACGAG CGGAAGCGGA GACTTGAGAA CATCGACAAG 
ATTGCTCAGT GGCAGAGCTC CATAGAGGAC TGGGAGGGAG AAGATCTCTT GGTCAGGAGC 
TCAGAACTCA TCTACTCGGG GGAGCTGACT CGAGTTACAC AGCCTCAAGC CAAAAGCCAG 
CAGCGAATGT TCTTTCTCTT TGACCACCAG CTCATCTACT GTAAGAAGGA CCTGCTCCGC 
CGCGACGTGT TGTACTACAA GGGCCGGCTG GACATGGACG GCCTGGAGGT GGTGGACCTG 
GAGGACGGGA AGGACAGAGA CCTCCATGTG AGCATCAAGA ACGCCTTCCG GCTGCACCGT 
GGCGCCACAG GGGACAGCCA CCTGCTGTGC ACCAGGAAGC CCGAGCAGAA GCAGCGCTGG 
CTCAAGGCCT TTGCCAGGGA GAGGGAGCAG GTGCAGCTGG ACCAGGAGAC AGGCTTCTCC 
ATCACTGAAC TGCAGAGGAA GCAGGCCATG CTGAATGCCA GCAAGCAGCA GGTCACAGGG 
AAGCCCAAAG CTGTTGGCCG GCCCTGCTAC CTGACGCGCC AGAAGCACCC AGCCCTGCCC 
AGCAACCGGC CCCAGCAGCA GGTCCTGGTG CTGGCGGAGC CCAGGCGCAA GCCATCTACC 
TTCTGGCACA GCATCAGCCG GCTGGCACCC TTCCGCAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF4 ( NM_001375903.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001362832.1 Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant 7, mRNA.

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CloneID	OHu109044
Clone ID Related Accession (Same CDS sequence)	NM_001375903.1
Accession Version	NM_001375903.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1863bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-05
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 4 isoform g
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC112786.4 and AC009477.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.212252.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

ATGATGTCAG CCGGCCCGCA CGCCCCTCGC CCTCCGACTG CGACCCCGGC CCGGGCTCGC 
GCGCCCCGTC CCCCGGCAGC CCCGCGGCCG CCGCACCGCC GCCCCCGGCC CAGCCTTCCC 
CGAGCCTGTG GCTGGAGCTC GGGCCCGCCT GCGTGCGGGC GCAGCAATGC CCCAGCGAGT 
CAAGCGGGCA GACGAGTGGC GATCTCGGCA CTAGCAGCAG CAGCAGCGCC GGGCTGTCCC 
CGGGCTCCGA CTCGGACAGC AGCGGCGTGG TGTGTGGCGG CCGCGGAGGC AACGGGGGCA 
TGCGCGGCGC CGTGTCCCGC TCCTGGAGCC TGGAGAGCCT GCGCTCGGCC ACCGCCGCTC 
ATCAGCGATG GCAGTGTGGT CTGCGCTGAA GCACTCTGGG ACCATGTCAC CATGGACGAC 
CAGGAGCTGG GCTTCAAAGC TGGGGACGTC ATCGAAGTGA TGGATGCCAC CAACAGAGAG 
TGGTGGTGGG GCCGGGTCGC CGATGGCGAG GGCTGGTTTC CAGCCAGCTT CGTTCGGCTG 
AGGGTGAATC AGGACGAGCC CGCGGATGAC GACGCCCCTC TGGCCGGGAA CAGCGGAGCG 
GAGGACGGCG GGGCGGAGGC GCAGAGCAGC AAGGACCAGA TGCGGACCAA CGTCATCAAC 
GAGATCCTCA GCACTGAGCG GGACTACATC AAGCACCTGC GCGACATCTG CGAGGGCTAC 
GTCCGGCAGT GCCGCAAGCG CGCAGACATG TTCAGCGAGG AGCAGCTGCG TACCATCTTC 
GGGAACATCG AGGACATCTA CCGCTGCCAG AAGGCCTTCG TGAAGGCCCT GGAGCAGAGG 
TTCAACCGCG AGCGCCCACA CCTGAGCGAG CTGGGTGCCT GCTTCCTGGA GCATCAAGCC 
GACTTCCAGA TCTACTCGGA GTACTGCAAT AACCACCCCA ACGCCTGCGT GGAGCTCTCC 
CGGCTCACCA AGCTCAGCAA GTACGTGTAC TTCTTCGAGG CCTGCCGGCT GCTGCAGAAG 
ATGATTGACA TCTCCCTGGA TGGCTTCCTG CTGACTCCGG TGCAGAAGAT CTGCAAGTAC 
CCTCTGCAGC TGGCCGAGCT GCTCAAATAC ACGCACCCCC AGCACAGGGA CTTCAAGGAT 
GTTGAAGCCG CCTTGCATGC CATGAAGAAC GTGGCCCAGC TCATCAACGA GCGGAAGCGG 
AGACTTGAGA ACATCGACAA GATTGCTCAG TGGCAGAGCT CCATAGAGGA CTGGGAGGGA 
GAAGATCTCT TGGTCAGGAG CTCAGAACTC ATCTACTCGG GGGAGCTGAC TCGAGTTACA 
CAGCCTCAAG CCAAAAGCCA GCAGCGAATG TTCTTTCTCT TTGACCACCA GCTCATCTAC 
TGTAAGAAGG ACCTGCTCCG CCGCGACGTG TTGTACTACA AGGGCCGGCT GGACATGGAC 
GGCCTGGAGG TGGTGGACCT GGAGGACGGG AAGGACAGAG ACCTCCATGT GAGCATCAAG 
AACGCCTTCC GGCTGCACCG TGGCGCCACA GGGGACAGCC ACCTGCTGTG CACCAGGAAG 
CCCGAGCAGA AGCAGCGCTG GCTCAAGGCC TTTGCCAGGG AGAGGGAGCA GGTGCAGCTG 
GACCAGGAGA CAGGCTTCTC CATCACTGAA CTGCAGAGGA AGCAGGCCAT GCTGAATGCC 
AGCAAGCAGC AGGTCACAGG GAAGCCCAAA GCTGTTGGCC GGCCCTGCTA CCTGACGCGC 
CAGAAGCACC CAGCCCTGCC CAGCAACCGG CCCCAGCAGC AGGTCCTGGT GCTGGCGGAG 
CCCAGGCGCA AGCCATCTAC CTTCTGGCAC AGCATCAGCC GGCTGGCACC CTTCCGCAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001362832.1
CDS	239..2101
Translation	MMSAGPHAPRPPTATPARARAPRPPAAPRPPHRRPRPSLPRACGWSSGPPACGRSNAPASQAGRRVAISALAAAAAPGCPRAPTRTAAAWCVAAAEATGACAAPCPAPGAWRACARPPPLISDGSVVCAEALWDHVTMDDQELGFKAGDVIEVMDATNREWWWGRVADGEGWFPASFVRLRVNQDEPADDDAPLAGNSGAEDGGAEAQSSKDQMRTNVINEILSTERDYIKHLRDICEGYVRQCRKRADMFSEEQLRTIFGNIEDIYRCQKAFVKALEQRFNRERPHLSELGACFLEHQADFQIYSEYCNNHPNACVELSRLTKLSKYVYFFEACRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTHPQHRDFKDVEAALHAMKNVAQLINERKRRLENIDKIAQWQSSIEDWEGEDLLVRSSELIYSGELTRVTQPQAKSQQRMFFLFDHQLIYCKKDLLRRDVLYYKGRLDMDGLEVVDLEDGKDRDLHVSIKNAFRLHRGATGDSHLLCTRKPEQKQRWLKAFAREREQVQLDQETGFSITELQRKQAMLNASKQQVTGKPKAVGRPCYLTRQKHPALPSNRPQQQVLVLAEPRRKPSTFWHSISRLAPFRK

Target ORF information:

RefSeq Version	NM_001375903.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001375903.1

ORF Insert Sequence:

ATGATGTCAG CCGGCCCGCA CGCCCCTCGC CCTCCGACTG CGACCCCGGC CCGGGCTCGC 
GCGCCCCGTC CCCCGGCAGC CCCGCGGCCG CCGCACCGCC GCCCCCGGCC CAGCCTTCCC 
CGAGCCTGTG GCTGGAGCTC GGGCCCGCCT GCGTGCGGGC GCAGCAATGC CCCAGCGAGT 
CAAGCGGGCA GACGAGTGGC GATCTCGGCA CTAGCAGCAG CAGCAGCGCC GGGCTGTCCC 
CGGGCTCCGA CTCGGACAGC AGCGGCGTGG TGTGTGGCGG CCGCGGAGGC AACGGGGGCA 
TGCGCGGCGC CGTGTCCCGC TCCTGGAGCC TGGAGAGCCT GCGCTCGGCC ACCGCCGCTC 
ATCAGCGATG GCAGTGTGGT CTGCGCTGAA GCACTCTGGG ACCATGTCAC CATGGACGAC 
CAGGAGCTGG GCTTCAAAGC TGGGGACGTC ATCGAAGTGA TGGATGCCAC CAACAGAGAG 
TGGTGGTGGG GCCGGGTCGC CGATGGCGAG GGCTGGTTTC CAGCCAGCTT CGTTCGGCTG 
AGGGTGAATC AGGACGAGCC CGCGGATGAC GACGCCCCTC TGGCCGGGAA CAGCGGAGCG 
GAGGACGGCG GGGCGGAGGC GCAGAGCAGC AAGGACCAGA TGCGGACCAA CGTCATCAAC 
GAGATCCTCA GCACTGAGCG GGACTACATC AAGCACCTGC GCGACATCTG CGAGGGCTAC 
GTCCGGCAGT GCCGCAAGCG CGCAGACATG TTCAGCGAGG AGCAGCTGCG TACCATCTTC 
GGGAACATCG AGGACATCTA CCGCTGCCAG AAGGCCTTCG TGAAGGCCCT GGAGCAGAGG 
TTCAACCGCG AGCGCCCACA CCTGAGCGAG CTGGGTGCCT GCTTCCTGGA GCATCAAGCC 
GACTTCCAGA TCTACTCGGA GTACTGCAAT AACCACCCCA ACGCCTGCGT GGAGCTCTCC 
CGGCTCACCA AGCTCAGCAA GTACGTGTAC TTCTTCGAGG CCTGCCGGCT GCTGCAGAAG 
ATGATTGACA TCTCCCTGGA TGGCTTCCTG CTGACTCCGG TGCAGAAGAT CTGCAAGTAC 
CCTCTGCAGC TGGCCGAGCT GCTCAAATAC ACGCACCCCC AGCACAGGGA CTTCAAGGAT 
GTTGAAGCCG CCTTGCATGC CATGAAGAAC GTGGCCCAGC TCATCAACGA GCGGAAGCGG 
AGACTTGAGA ACATCGACAA GATTGCTCAG TGGCAGAGCT CCATAGAGGA CTGGGAGGGA 
GAAGATCTCT TGGTCAGGAG CTCAGAACTC ATCTACTCGG GGGAGCTGAC TCGAGTTACA 
CAGCCTCAAG CCAAAAGCCA GCAGCGAATG TTCTTTCTCT TTGACCACCA GCTCATCTAC 
TGTAAGAAGG ACCTGCTCCG CCGCGACGTG TTGTACTACA AGGGCCGGCT GGACATGGAC 
GGCCTGGAGG TGGTGGACCT GGAGGACGGG AAGGACAGAG ACCTCCATGT GAGCATCAAG 
AACGCCTTCC GGCTGCACCG TGGCGCCACA GGGGACAGCC ACCTGCTGTG CACCAGGAAG 
CCCGAGCAGA AGCAGCGCTG GCTCAAGGCC TTTGCCAGGG AGAGGGAGCA GGTGCAGCTG 
GACCAGGAGA CAGGCTTCTC CATCACTGAA CTGCAGAGGA AGCAGGCCAT GCTGAATGCC 
AGCAAGCAGC AGGTCACAGG GAAGCCCAAA GCTGTTGGCC GGCCCTGCTA CCTGACGCGC 
CAGAAGCACC CAGCCCTGCC CAGCAACCGG CCCCAGCAGC AGGTCCTGGT GCTGGCGGAG 
CCCAGGCGCA AGCCATCTAC CTTCTGGCAC AGCATCAGCC GGCTGGCACC CTTCCGCAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF4 ( NM_001375902.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001362831.1 Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant 6, mRNA.

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CloneID	OHu109045
Clone ID Related Accession (Same CDS sequence)	NM_001375902.1
Accession Version	NM_001375902.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-05
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 4 isoform f
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC112786.4 and AC009477.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.204701.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

ATGATGTCAG CCGGCCCGCA CGCCCCTCGC CCTCCGACTG CGACCCCGGC CCGGGCTCGC 
GCGCCCCGTC CCCCGGCAGC CCCGCGGCCG CCGCACCGCC GCCCCCGGCC CAGCCTTCCC 
CGAGCCTGTG GCTGGAGCTC GGGCCCGCCT GCGTGCGGGC GCAGCAATGC CCCAGCGAGT 
CAAGCGGGCA GACGAGTGGC GATCTCGGCA CTAGCAGCAG CAGCAGCGCC GGGCTGTCCC 
CGGGCTCCGA CTCGGACAGC AGCGGCGTGG TGTGTGGCGG CCGCGGAGGC AACGGGGGCA 
TGCGCGGCGC CGTGTCCCGC TCCTGGAGCC TGGAGAGCCT GCGCTCGGCC ACCGCCGCCG 
GGACCTTGTT GGCCCCCATA CCAGAGCGCG CTGTCAACTG GGAAACTGAT TAAGCTCATC 
AGCGATGGCA GTGTGGTCTG CGCTGAAGCA CTCTGGGACC ATGTCACCAT GGACGACCAG 
GAGCTGGGCT TCAAAGCTGG GGACGTCATC GAAGTGATGG ATGCCACCAA CAGAGAGTGG 
TGGTGGGGCC GGGTCGCCGA TGGCGAGGGC TGGTTTCCAG CCAGCTTCGT TCGGCTGAGG 
GTGAATCAGG ACGAGCCCGC GGATGACGAC GCCCCTCTGG CCGGGAACAG CGGAGCGGAG 
GACGGCGGGG CGGAGGCGCA GAGCAGCAAG GACCAGATGC GGACCAACGT CATCAACGAG 
ATCCTCAGCA CTGAGCGGGA CTACATCAAG CACCTGCGCG ACATCTGCGA GGGCTACGTC 
CGGCAGTGCC GCAAGCGCGC AGACATGTTC AGCGAGGAGC AGCTGCGTAC CATCTTCGGG 
AACATCGAGG ACATCTACCG CTGCCAGAAG GCCTTCGTGA AGGCCCTGGA GCAGAGGTTC 
AACCGCGAGC GCCCACACCT GAGCGAGCTG GGTGCCTGCT TCCTGGAGCA TCAAGCCGAC 
TTCCAGATCT ACTCGGAGTA CTGCAATAAC CACCCCAACG CCTGCGTGGA GCTCTCCCGG 
CTCACCAAGC TCAGCAAGTA CGTGTACTTC TTCGAGGCCT GCCGGCTGCT GCAGAAGATG 
ATTGACATCT CCCTGGATGG CTTCCTGCTG ACTCCGGTGC AGAAGATCTG CAAGTACCCT 
CTGCAGCTGG CCGAGCTGCT CAAATACACG CACCCCCAGC ACAGGGACTT CAAGGATGTT 
GAAGCCGCCT TGCATGCCAT GAAGAACGTG GCCCAGCTCA TCAACGAGCG GAAGCGGAGA 
CTTGAGAACA TCGACAAGAT TGCTCAGTGG CAGAGCTCCA TAGAGGACTG GGAGGGAGAA 
GATCTCTTGG TCAGGAGCTC AGAACTCATC TACTCGGGGG AGCTGACTCG AGTTACACAG 
CCTCAAGCCA AAAGCCAGCA GCGAATGTTC TTTCTCTTTG ACCACCAGCT CATCTACTGT 
AAGAAGGACC TGCTCCGCCG CGACGTGTTG TACTACAAGG GCCGGCTGGA CATGGACGGC 
CTGGAGGTGG TGGACCTGGA GGACGGGAAG GACAGAGACC TCCATGTGAG CATCAAGAAC 
GCCTTCCGGC TGCACCGTGG CGCCACAGGG GACAGCCACC TGCTGTGCAC CAGGAAGCCC 
GAGCAGAAGC AGCGCTGGCT CAAGGCCTTT GCCAGGGAGA GGGAGCAGGT GCAGCTGGAC 
CAGGAGACAG GCTTCTCCAT CACTGAACTG CAGAGGAAGC AGGCCATGCT GAATGCCAGC 
AAGCAGCAGG TCACAGGGAA GCCCAAAGCT GTTGGCCGGC CCTGCTACCT GACGCGCCAG 
AAGCACCCAG CCCTGCCCAG CAACCGGCCC CAGCAGCAGG TCCTGGTGCT GGCGGAGCCC 
AGGCGCAAGC CATCTACCTT CTGGCACAGC ATCAGCCGGC TGGCACCCTT CCGCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001362831.1
CDS	239..2158
Translation	MMSAGPHAPRPPTATPARARAPRPPAAPRPPHRRPRPSLPRACGWSSGPPACGRSNAPASQAGRRVAISALAAAAAPGCPRAPTRTAAAWCVAAAEATGACAAPCPAPGAWRACARPPPPGPCWPPYQSALSTGKLIKLISDGSVVCAEALWDHVTMDDQELGFKAGDVIEVMDATNREWWWGRVADGEGWFPASFVRLRVNQDEPADDDAPLAGNSGAEDGGAEAQSSKDQMRTNVINEILSTERDYIKHLRDICEGYVRQCRKRADMFSEEQLRTIFGNIEDIYRCQKAFVKALEQRFNRERPHLSELGACFLEHQADFQIYSEYCNNHPNACVELSRLTKLSKYVYFFEACRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTHPQHRDFKDVEAALHAMKNVAQLINERKRRLENIDKIAQWQSSIEDWEGEDLLVRSSELIYSGELTRVTQPQAKSQQRMFFLFDHQLIYCKKDLLRRDVLYYKGRLDMDGLEVVDLEDGKDRDLHVSIKNAFRLHRGATGDSHLLCTRKPEQKQRWLKAFAREREQVQLDQETGFSITELQRKQAMLNASKQQVTGKPKAVGRPCYLTRQKHPALPSNRPQQQVLVLAEPRRKPSTFWHSISRLAPFRK

Target ORF information:

RefSeq Version	NM_001375902.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001375902.1

ORF Insert Sequence:

ATGATGTCAG CCGGCCCGCA CGCCCCTCGC CCTCCGACTG CGACCCCGGC CCGGGCTCGC 
GCGCCCCGTC CCCCGGCAGC CCCGCGGCCG CCGCACCGCC GCCCCCGGCC CAGCCTTCCC 
CGAGCCTGTG GCTGGAGCTC GGGCCCGCCT GCGTGCGGGC GCAGCAATGC CCCAGCGAGT 
CAAGCGGGCA GACGAGTGGC GATCTCGGCA CTAGCAGCAG CAGCAGCGCC GGGCTGTCCC 
CGGGCTCCGA CTCGGACAGC AGCGGCGTGG TGTGTGGCGG CCGCGGAGGC AACGGGGGCA 
TGCGCGGCGC CGTGTCCCGC TCCTGGAGCC TGGAGAGCCT GCGCTCGGCC ACCGCCGCCG 
GGACCTTGTT GGCCCCCATA CCAGAGCGCG CTGTCAACTG GGAAACTGAT TAAGCTCATC 
AGCGATGGCA GTGTGGTCTG CGCTGAAGCA CTCTGGGACC ATGTCACCAT GGACGACCAG 
GAGCTGGGCT TCAAAGCTGG GGACGTCATC GAAGTGATGG ATGCCACCAA CAGAGAGTGG 
TGGTGGGGCC GGGTCGCCGA TGGCGAGGGC TGGTTTCCAG CCAGCTTCGT TCGGCTGAGG 
GTGAATCAGG ACGAGCCCGC GGATGACGAC GCCCCTCTGG CCGGGAACAG CGGAGCGGAG 
GACGGCGGGG CGGAGGCGCA GAGCAGCAAG GACCAGATGC GGACCAACGT CATCAACGAG 
ATCCTCAGCA CTGAGCGGGA CTACATCAAG CACCTGCGCG ACATCTGCGA GGGCTACGTC 
CGGCAGTGCC GCAAGCGCGC AGACATGTTC AGCGAGGAGC AGCTGCGTAC CATCTTCGGG 
AACATCGAGG ACATCTACCG CTGCCAGAAG GCCTTCGTGA AGGCCCTGGA GCAGAGGTTC 
AACCGCGAGC GCCCACACCT GAGCGAGCTG GGTGCCTGCT TCCTGGAGCA TCAAGCCGAC 
TTCCAGATCT ACTCGGAGTA CTGCAATAAC CACCCCAACG CCTGCGTGGA GCTCTCCCGG 
CTCACCAAGC TCAGCAAGTA CGTGTACTTC TTCGAGGCCT GCCGGCTGCT GCAGAAGATG 
ATTGACATCT CCCTGGATGG CTTCCTGCTG ACTCCGGTGC AGAAGATCTG CAAGTACCCT 
CTGCAGCTGG CCGAGCTGCT CAAATACACG CACCCCCAGC ACAGGGACTT CAAGGATGTT 
GAAGCCGCCT TGCATGCCAT GAAGAACGTG GCCCAGCTCA TCAACGAGCG GAAGCGGAGA 
CTTGAGAACA TCGACAAGAT TGCTCAGTGG CAGAGCTCCA TAGAGGACTG GGAGGGAGAA 
GATCTCTTGG TCAGGAGCTC AGAACTCATC TACTCGGGGG AGCTGACTCG AGTTACACAG 
CCTCAAGCCA AAAGCCAGCA GCGAATGTTC TTTCTCTTTG ACCACCAGCT CATCTACTGT 
AAGAAGGACC TGCTCCGCCG CGACGTGTTG TACTACAAGG GCCGGCTGGA CATGGACGGC 
CTGGAGGTGG TGGACCTGGA GGACGGGAAG GACAGAGACC TCCATGTGAG CATCAAGAAC 
GCCTTCCGGC TGCACCGTGG CGCCACAGGG GACAGCCACC TGCTGTGCAC CAGGAAGCCC 
GAGCAGAAGC AGCGCTGGCT CAAGGCCTTT GCCAGGGAGA GGGAGCAGGT GCAGCTGGAC 
CAGGAGACAG GCTTCTCCAT CACTGAACTG CAGAGGAAGC AGGCCATGCT GAATGCCAGC 
AAGCAGCAGG TCACAGGGAA GCCCAAAGCT GTTGGCCGGC CCTGCTACCT GACGCGCCAG 
AAGCACCCAG CCCTGCCCAG CAACCGGCCC CAGCAGCAGG TCCTGGTGCT GGCGGAGCCC 
AGGCGCAAGC CATCTACCTT CTGGCACAGC ATCAGCCGGC TGGCACCCTT CCGCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF4 ( NM_001375904.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001362833.1 Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant 8, mRNA.

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CloneID	OHu36332
Clone ID Related Accession (Same CDS sequence)	XM_005263687.4 , NM_001375904.1 , XM_005263687.3
Accession Version	NM_001375904.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-05
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 4 isoform h
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC112786.4 and AC009477.4. On Nov 6, 2019 this sequence version replaced XM_005263687.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.238693.1, SRR1803611.250503.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCGCGGCG CCGTGTCCCG CTCCTGGAGC CTGGAGAGCC TGCGCTCGGC CACCGCCGCC 
ATGCCTGATG GAGCTCTGGA CACAGCTGTC TGCGCTGACG AAGTGGGGAG CGAGGAGGAC 
CTGTATGATG ACCTGCACAG CTCCAGCCAC CACTACAGCC ACCCTGGAGG GGGTGGGGAG 
CAGCTGGCTA TCAATGAGCT CATCAGCGAT GGCAGTGTGG TCTGCGCTGA AGCACTCTGG 
GACCATGTCA CCATGGACGA CCAGGAGCTG GGCTTCAAAG CTGGGGACGT CATCGAAGTG 
ATGGATGCCA CCAACAGAGA GTGGTGGTGG GGCCGGGTCG CCGATGGCGA GGGCTGGTTT 
CCAGCCAGCT TCGTTCGGCT GAGGGTGAAT CAGGACGAGC CCGCGGATGA CGACGCCCCT 
CTGGCCGGGA ACAGCGGAGC GGAGGACGGC GGGGCGGAGG CGCAGAGCAG CAAGGACCAG 
ATGCGGACCA ACGTCATCAA CGAGATCCTC AGCACTGAGC GGGACTACAT CAAGCACCTG 
CGCGACATCT GCGAGGGCTA CGTCCGGCAG TGCCGCAAGC GCGCAGACAT GTTCAGCGAG 
GAGCAGCTGC GTACCATCTT CGGGAACATC GAGGACATCT ACCGCTGCCA GAAGGCCTTC 
GTGAAGGCCC TGGAGCAGAG GTTCAACCGC GAGCGCCCAC ACCTGAGCGA GCTGGGTGCC 
TGCTTCCTGG AGCATCAAGC CGACTTCCAG ATCTACTCGG AGTACTGCAA TAACCACCCC 
AACGCCTGCG TGGAGCTCTC CCGGCTCACC AAGCTCAGCA AGTACGTGTA CTTCTTCGAG 
GCCTGCCGGC TGCTGCAGAA GATGATTGAC ATCTCCCTGG ATGGCTTCCT GCTGACTCCG 
GTGCAGAAGA TCTGCAAGTA CCCTCTGCAG CTGGCCGAGC TGCTCAAATA CACGCACCCC 
CAGCACAGGG ACTTCAAGGA TGTTGAAGCC GCCTTGCATG CCATGAAGAA CGTGGCCCAG 
CTCATCAACG AGCGGAAGCG GAGACTTGAG AACATCGACA AGATTGCTCA GTGGCAGAGC 
TCCATAGAGG ACTGGGAGGG AGAAGATCTC TTGGTCAGGA GCTCAGAACT CATCTACTCG 
GGGGAGCTGA CTCGAGTTAC ACAGCCTCAA GCCAAAAGCC AGCAGCGAAT GTTCTTTCTC 
TTTGACCACC AGCTCATCTA CTGTAAGAAG GACCTGCTCC GCCGCGACGT GTTGTACTAC 
AAGGGCCGGC TGGACATGGA CGGCCTGGAG GTGGTGGACC TGGAGGACGG GAAGGACAGA 
GACCTCCATG TGAGCATCAA GAACGCCTTC CGGCTGCACC GTGGCGCCAC AGGGGACAGC 
CACCTGCTGT GCACCAGGAA GCCCGAGCAG AAGCAGCGCT GGCTCAAGGC CTTTGCCAGG 
GAGAGGGAGC AGGTGCAGCT GGACCAGGAG ACAGGCTTCT CCATCACTGA ACTGCAGAGG 
AAGCAGGCCA TGCTGAATGC CAGCAAGCAG CAGGTCACAG GGAAGCCCAA AGCTGTTGGC 
CGGCCCTGCT ACCTGACGCG CCAGAAGCAC CCAGCCCTGC CCAGCAACCG GCCCCAGCAG 
CAGGTCCTGG TGCTGGCGGA GCCCAGGCGC AAGCCATCTA CCTTCTGGCA CAGCATCAGC 
CGGCTGGCAC CCTTCCGCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001362833.1
CDS	538..2241
Translation	MRGAVSRSWSLESLRSATAAMPDGALDTAVCADEVGSEEDLYDDLHSSSHHYSHPGGGGEQLAINELISDGSVVCAEALWDHVTMDDQELGFKAGDVIEVMDATNREWWWGRVADGEGWFPASFVRLRVNQDEPADDDAPLAGNSGAEDGGAEAQSSKDQMRTNVINEILSTERDYIKHLRDICEGYVRQCRKRADMFSEEQLRTIFGNIEDIYRCQKAFVKALEQRFNRERPHLSELGACFLEHQADFQIYSEYCNNHPNACVELSRLTKLSKYVYFFEACRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTHPQHRDFKDVEAALHAMKNVAQLINERKRRLENIDKIAQWQSSIEDWEGEDLLVRSSELIYSGELTRVTQPQAKSQQRMFFLFDHQLIYCKKDLLRRDVLYYKGRLDMDGLEVVDLEDGKDRDLHVSIKNAFRLHRGATGDSHLLCTRKPEQKQRWLKAFAREREQVQLDQETGFSITELQRKQAMLNASKQQVTGKPKAVGRPCYLTRQKHPALPSNRPQQQVLVLAEPRRKPSTFWHSISRLAPFRK

Target ORF information:

RefSeq Version	NM_001375904.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001375904.1

ORF Insert Sequence:

ATGCGCGGCG CCGTGTCCCG CTCCTGGAGC CTGGAGAGCC TGCGCTCGGC CACCGCCGCC 
ATGCCTGATG GAGCTCTGGA CACAGCTGTC TGCGCTGACG AAGTGGGGAG CGAGGAGGAC 
CTGTATGATG ACCTGCACAG CTCCAGCCAC CACTACAGCC ACCCTGGAGG GGGTGGGGAG 
CAGCTGGCTA TCAATGAGCT CATCAGCGAT GGCAGTGTGG TCTGCGCTGA AGCACTCTGG 
GACCATGTCA CCATGGACGA CCAGGAGCTG GGCTTCAAAG CTGGGGACGT CATCGAAGTG 
ATGGATGCCA CCAACAGAGA GTGGTGGTGG GGCCGGGTCG CCGATGGCGA GGGCTGGTTT 
CCAGCCAGCT TCGTTCGGCT GAGGGTGAAT CAGGACGAGC CCGCGGATGA CGACGCCCCT 
CTGGCCGGGA ACAGCGGAGC GGAGGACGGC GGGGCGGAGG CGCAGAGCAG CAAGGACCAG 
ATGCGGACCA ACGTCATCAA CGAGATCCTC AGCACTGAGC GGGACTACAT CAAGCACCTG 
CGCGACATCT GCGAGGGCTA CGTCCGGCAG TGCCGCAAGC GCGCAGACAT GTTCAGCGAG 
GAGCAGCTGC GTACCATCTT CGGGAACATC GAGGACATCT ACCGCTGCCA GAAGGCCTTC 
GTGAAGGCCC TGGAGCAGAG GTTCAACCGC GAGCGCCCAC ACCTGAGCGA GCTGGGTGCC 
TGCTTCCTGG AGCATCAAGC CGACTTCCAG ATCTACTCGG AGTACTGCAA TAACCACCCC 
AACGCCTGCG TGGAGCTCTC CCGGCTCACC AAGCTCAGCA AGTACGTGTA CTTCTTCGAG 
GCCTGCCGGC TGCTGCAGAA GATGATTGAC ATCTCCCTGG ATGGCTTCCT GCTGACTCCG 
GTGCAGAAGA TCTGCAAGTA CCCTCTGCAG CTGGCCGAGC TGCTCAAATA CACGCACCCC 
CAGCACAGGG ACTTCAAGGA TGTTGAAGCC GCCTTGCATG CCATGAAGAA CGTGGCCCAG 
CTCATCAACG AGCGGAAGCG GAGACTTGAG AACATCGACA AGATTGCTCA GTGGCAGAGC 
TCCATAGAGG ACTGGGAGGG AGAAGATCTC TTGGTCAGGA GCTCAGAACT CATCTACTCG 
GGGGAGCTGA CTCGAGTTAC ACAGCCTCAA GCCAAAAGCC AGCAGCGAAT GTTCTTTCTC 
TTTGACCACC AGCTCATCTA CTGTAAGAAG GACCTGCTCC GCCGCGACGT GTTGTACTAC 
AAGGGCCGGC TGGACATGGA CGGCCTGGAG GTGGTGGACC TGGAGGACGG GAAGGACAGA 
GACCTCCATG TGAGCATCAA GAACGCCTTC CGGCTGCACC GTGGCGCCAC AGGGGACAGC 
CACCTGCTGT GCACCAGGAA GCCCGAGCAG AAGCAGCGCT GGCTCAAGGC CTTTGCCAGG 
GAGAGGGAGC AGGTGCAGCT GGACCAGGAG ACAGGCTTCT CCATCACTGA ACTGCAGAGG 
AAGCAGGCCA TGCTGAATGC CAGCAAGCAG CAGGTCACAG GGAAGCCCAA AGCTGTTGGC 
CGGCCCTGCT ACCTGACGCG CCAGAAGCAC CCAGCCCTGC CCAGCAACCG GCCCCAGCAG 
CAGGTCCTGG TGCTGGCGGA GCCCAGGCGC AAGCCATCTA CCTTCTGGCA CAGCATCAGC 
CGGCTGGCAC CCTTCCGCAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF4 ( NM_001367493.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001354422.1 Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant 3, mRNA.

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CloneID	OHu36328
Clone ID Related Accession (Same CDS sequence)	NM_001367493.1 , XM_005263681.1
Accession Version	NM_001367493.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-06
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 4 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC133785.2, AC112786.4, BC139742.1, AB042199.1 and AC009477.4. On Dec 4, 2018 this sequence version replaced XM_005263681.2. Transcript Variant: This variant (3) represents the longest transcript and encodes the longest isoform (c). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2144120 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTCAGCG TCGTGCACTT CCTCCGGAGC TTCTTCAAGA CTCCAGAGCC AGGTGCACAC 
CTGCCAGGTG AAGGTGAGAT TGAAGATAAC CAGCTCCCCA CATCCCCTGC AGAACAAGTG 
GAGCAGGGAT GGAACCAGCA AACAGACCGC GATGATTCTG AAACGCTGTC CCAGCAGAGT 
GAAAGTGGAT CAGACACAAA AACTGACCCC TTTGAAAGTG CCTCTGACAC AGAGTCCTTG 
TCTGGGTACC TCCCGAGGGG AGTCTTCCAC CCTCTAAGAG GTACTCCCGT AGATATAGAA 
GCACCTTGGG AATACCCTGA TGTCTCAGCA ACTGGACCCC CTCAGGAGCA GCATTTGACC 
AGTGTTCCTG GGCTTCATGC AAAGGAAGAA CTCGATTTGT CCCCTAGCTT AGAGGATGAC 
TCTTGCAAAA ATGGGTGGCG AGCCTTTGCC ACAGTTGCTG GAGAACAGGA GGCAGGACAC 
CTCTGGGACT GCGCGACCAG CCTGGAGCGA GAGTCTTTGC TGGCAGGGGT TCCCCGACAC 
ACAGGGTGCT GCTTACAGAG GGCCACAGAC AGCAGTGGTC CTGAGCCAGT ACAGGGGGTG 
GCTGTTCAAG ACCTCAGAGG GCTCTCCAGT GTTTCTCTTC AGAAATCCAG GTCTGAGAGC 
TATCTGGGCA TCCCAGTGGT CTGGCCCTTC CTTCTCTGGT GCTGTGAACT GGGTCGGAGT 
TGGCCACATA TCCACAACAG GGCCAGGGCA CTGGTGCTAC CTAGCAGAGG CCCACTGGAC 
AACACAGCCC CTCTGGGGAC CAGGACAAAG AAGGAAAGTA CTCTAGGCCC TGCAGGGGAC 
ACAGAATTGC TCTGGTCCCA GCCCCACTCG GATGTCCCCT GCCAGCCTCC TTTGAGGACA 
TCTTGCCTCC TACGCACCAA CCGTCACCAT AGTGCCCCAG AAACTACTGG TGACAAGAAC 
AGGGCATCCC CCAGACTCAA CTGTGGGCAC ATGAGGGCAC TGGTCAGGGC CCATTCCATA 
GCAGGGTTTT CACCAGAGTG CCCCGAGGAT CCCGTGGGAC AGAATGTTGT GAAGTCTGGG 
ACCCATGTGA AGGAAGGGGC CAAAAATGAA CGAGATCCAA GAATACAAAA CATCCCTTCC 
CCTGCACCCA CCCAGCTGTC TGGCCCGATT CCTGCTTTTC AGAGTGGGGC TCCCCATCTG 
CAGGGTCCCT GCAAGCCTGG TGGGTTTCGC TTGCAAAGGG CCTCTCAGGA CACTCCTTCT 
GCAGGTCTCC TGGGGGAAAA CCAGTTAAGA CAGGATTCCA GGTCATGTCT GGTGGCTTCA 
TGCCTCACCT CAGAGTTAGT GAAGCTCAGT GCAGAGGAAG TGCCTGAGCC CGCTGAGTGC 
AAGTCAGAGC AAAGCCCAGA AAGCAGAACC CAGGAACCCC AAGGCACCCA ACTCGCACCA 
AGAGCTGCTG ATGAGAGAGA GACACAGAAG CACCTCTGGG GCATTTCTGT CCAGGCAGGA 
AACCAAACCA GTAATTACAC ATCAAAGTAT GTGCTCAGCG AGGAAAGCAA GTCACCTACC 
AGGGCCAAGT TCCCACGGCA GCCTAGCAGT GAGGGGACCC AGGTGTGGAG TGGAGACTTG 
ATGGGCTGCT TGGAAGTGAG TGACAGTTCA GATGCCCCTG AGACCACCCA GAAATCAAGC 
GCAATAGACA CTTCAAAGGC AGCCGAAGAA GCCATGGTTC TAGACCCCAA CTACAGGGAA 
CAGGCTTTGC AAGGTCTTTC AGAATTCAAG GCAGCCACGG TGTCTCACTG CGGCCCCGGG 
GCTGAGGAGG GTGAACAGGG GCCTGGGGGT GCCGGGGGCC GGCAGCTGGA GCCCAAAGCA 
GGCGGCGAGG CCTCGAGGGG CAGGGGCGCC CTCATCATTG TAGCTGTGGA GCAGAAAGGT 
CTTCAGGCCA GCAGGAGCAA TGCGGGGCCT GTTCCAGAAA CACTGCCCCG TGACTTTCCT 
AAGGAAAGAC CAGAATCTCC CTTGAGCACT GGCGAGACCC CCTGTGAGTC TCCCACTAGG 
GGGAAAACAC CAGCCGGTAA TGAGTGTGAG TTGCCAGCAG CCCCCATACA GGGAGCTGGC 
GATGGGGCTC TTCAGCGGGT GGCCCAGGCC GCAGAGCTTG GGAGAGTGCT GGTCCCCCAA 
GCTGCTTCGG AAGAGACGCC GAGCACAGAG GAGCCCCCGG GAGAGAGACT GCGTGGGGAG 
AGCCGGAGCT CCGGGTCAGG GGAGCGTGGC CCGGAGGAGG CCCCCGAAGG CGGTGCTGCA 
GCAGCCCGGG GCCAGCGCCC CCGCGTCCCC GCCTTGGAGC CGCCCCAGCC GCCACGCGGG 
CTCCGCAAGG GCGCGCAGGA GCCTGGGAAG CGCCCGACGT TTTCCAAGGT GACCTCCTTC 
AGGAAGGGCA GGCCCTTGGC CACTGAGAGC CCAGGAGGGG TCCCGGCCCC GACCACCGAG 
GGTCGCCGCT GGGGCTCTTC AGGCCCCGAG GGGCTCCCCA GGGAGAATCC GCCCGCTGCG 
GCCGGTCGGG ACGCACCGCC TCTGCACCAC GGGGACGCCA GCGCCTGGCC CGAGTTTGTC 
CCGCAGGCTG CAGGCGACAG GACTGCAGGG CCGGCAGGAG CGGGGCACAC GGGGACCTCA 
GGGGATCTTG GTAGCCGAGG GCCTTCCTCT GAGAGCTGCA ACGCAAAGAG ACTCAAAACA 
ACGGAGAAAA AACTCAGGGC AAGGTTGGCC TTGGCTCATA AGACCTTTTC AAACTTTATT 
GAGTCAATAG TTCTAGAGAA AGAGAACACC CATGAACGTT CCCCAAGTTC TCCCAAGGGC 
GAGAAGGAGA AGAGCAGGCT GCGCCAGGGT TCCTGGCGGG CGTTTCTGAA AAGCAAAGAT 
GCCGGAAGCC CCAAAAAGCC CACCCTAGTG AGTCTGCCTC TAGGACCCGA AGTTCTCTCC 
CCAGCAGAGA CCGACAGCCA CTGTGAGGAA CGGGCGGAGG ACAAAGAGGG CTATGTTTTT 
AGCGATCACT GGGCACCCCC ACTTGCCTCC ACACCTTTGT CCTCCAGTTT AGTTTCTCCA 
GAACACAGGA GGAAAAGTGA ACCGACCATC AAGTGCACAG CCACCCAGGA AGGCGGTAGG 
TACCTACCTT CAGGTATCTT TCCGGAAAAG TCCTGGCTGG CGTCCCCCGG CAGCCCTCGG 
GCCCAGCAGG CTGGAATCGC ACACACCCTG CCTTCCAGCT CTGCCTGCTG CCTGGCATAT 
GAAAACCCCG GGACGCCCTG CAGACCCACG AGCCCCAAGC CCCTGAGTCC CAGGCCTAGT 
GCTCAGCGGA TGGGTCTCCA CTACCCCGGG AGGGGTAGCG CCATCTCCAT GGTTTCTCTT 
GGAAGCTACA GCTACGTGGA CAGCAGTTCA GGGGACCCTG AAAGACCCAA GATTCCCAAG 
GGCCAGACCA GTTTCCTGCT TTCTCTGCAG ACGCTAAACC AAGATGAGCA GAAGGAAGAG 
AGCAGGGAAG GAGGCCAGGG TCCGCGCGGC TTGGGCACAG TGCCCTGGCT CAGGGACCTT 
CCTGGGAGTG AGAACCACAT GCCCTGGGAA GAACCAGCAG GTGAGAAGCC CAGTTGCTCT 
CACAGTCAGA AGGCGTTCCA CATGGAGCCT GCCCAGAAGC CCTGCTTCAC CACTGACATG 
GTGACATGGG CCCTCCTCTG CATCTCTGCA GAGACTGTGC GTGGGGAGGC TCCTTCACAG 
CCTAGGGGCA TCCCTCACCG CTCGCCCGTC AGTGTGGATG ACCTGTGGCT GGAGAAGACA 
CAGAGAAAGA AGTTGCAGAA GCAGGCCCAC GTCGAAAGGA GGCTGCACAT AGGGGCAGTG 
CACAAAGATG GAGTCAAGTG CTGGAGAAAG ACGATCATTA CCTCTCCAGA GTCTTTGAAT 
CTCCCTAGAA GAAGCCATCC ACTCTCCCAG AGTGCTCCAA CGGGACTGAA CCACATGGGC 
TGGCCAGAGC ACACACCAGG CACTGCCATG CCTGATGGAG CTCTGGACAC AGCTGTCTGC 
GCTGACGAAG TGGGGAGCGA GGAGGACCTG TATGATGACC TGCACAGCTC CAGCCACCAC 
TACAGCCACC CTGGAGGGGG TGGGGAGCAG CTGGCTATCA ATGAGCTCAT CAGCGATGGC 
AGTGTGGTCT GCGCTGAAGC ACTCTGGGAC CATGTCACCA TGGACGACCA GGAGCTGGGC 
TTCAAAGCTG GGGACGTCAT CGAAGTGATG GATGCCACCA ACAGAGAGTG GTGGTGGGGC 
CGGGTCGCCG ATGGCGAGGG CTGGTTTCCA GCCAGCTTCG TTCGGCTGAG GGTGAATCAG 
GACGAGCCCG CGGATGACGA CGCCCCTCTG GCCGGGAACA GCGGAGCGGA GGACGGCGGG 
GCGGAGGCGC AGAGCAGCAA GGACCAGATG CGGACCAACG TCATCAACGA GATCCTCAGC 
ACTGAGCGGG ACTACATCAA GCACCTGCGC GACATCTGCG AGGGCTACGT CCGGCAGTGC 
CGCAAGCGCG CAGACATGTT CAGCGAGGAG CAGCTGCGTA CCATCTTCGG GAACATCGAG 
GACATCTACC GCTGCCAGAA GGCCTTCGTG AAGGCCCTGG AGCAGAGGTT CAACCGCGAG 
CGCCCACACC TGAGCGAGCT GGGTGCCTGC TTCCTGGAGC ATCAAGCCGA CTTCCAGATC 
TACTCGGAGT ACTGCAATAA CCACCCCAAC GCCTGCGTGG AGCTCTCCCG GCTCACCAAG 
CTCAGCAAGT ACGTGTACTT CTTCGAGGCC TGCCGGCTGC TGCAGAAGAT GATTGACATC 
TCCCTGGATG GCTTCCTGCT GACTCCGGTG CAGAAGATCT GCAAGTACCC TCTGCAGCTG 
GCCGAGCTGC TCAAATACAC GCACCCCCAG CACAGGGACT TCAAGGATGT TGAAGCCGCC 
TTGCATGCCA TGAAGAACGT GGCCCAGCTC ATCAACGAGC GGAAGCGGAG ACTTGAGAAC 
ATCGACAAGA TTGCTCAGTG GCAGAGCTCC ATAGAGGACT GGGAGGGAGA AGATCTCTTG 
GTCAGGAGCT CAGAACTCAT CTACTCGGGG GAGCTGACTC GAGTTACACA GCCTCAAGCC 
AAAAGCCAGC AGCGAATGTT CTTTCTCTTT GACCACCAGC TCATCTACTG TAAGAAGGAC 
CTGCTCCGCC GCGACGTGTT GTACTACAAG GGCCGGCTGG ACATGGACGG CCTGGAGGTG 
GTGGACCTGG AGGACGGGAA GGACAGAGAC CTCCATGTGA GCATCAAGAA CGCCTTCCGG 
CTGCACCGTG GCGCCACAGG GGACAGCCAC CTGCTGTGCA CCAGGAAGCC CGAGCAGAAG 
CAGCGCTGGC TCAAGGCCTT TGCCAGGGAG AGGGAGCAGG TGCAGCTGGA CCAGGAGACA 
GGCTTCTCCA TCACTGAACT GCAGAGGAAG CAGGCCATGC TGAATGCCAG CAAGCAGCAG 
GTCACAGGGA AGCCCAAAGC TGTTGGCCGG CCCTGCTACC TGACGCGCCA GAAGCACCCA 
GCCCTGCCCA GCAACCGGCC CCAGCAGCAG GTCCTGGTGC TGGCGGAGCC CAGGCGCAAG 
CCATCTACCT TCTGGCACAG CATCAGCCGG CTGGCACCCT TCCGCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001354422.1
CDS	41..5671
Translation	MLSVVHFLRSFFKTPEPGAHLPGEGEIEDNQLPTSPAEQVEQGWNQQTDRDDSETLSQQSESGSDTKTDPFESASDTESLSGYLPRGVFHPLRGTPVDIEAPWEYPDVSATGPPQEQHLTSVPGLHAKEELDLSPSLEDDSCKNGWRAFATVAGEQEAGHLWDCATSLERESLLAGVPRHTGCCLQRATDSSGPEPVQGVAVQDLRGLSSVSLQKSRSESYLGIPVVWPFLLWCCELGRSWPHIHNRARALVLPSRGPLDNTAPLGTRTKKESTLGPAGDTELLWSQPHSDVPCQPPLRTSCLLRTNRHHSAPETTGDKNRASPRLNCGHMRALVRAHSIAGFSPECPEDPVGQNVVKSGTHVKEGAKNERDPRIQNIPSPAPTQLSGPIPAFQSGAPHLQGPCKPGGFRLQRASQDTPSAGLLGENQLRQDSRSCLVASCLTSELVKLSAEEVPEPAECKSEQSPESRTQEPQGTQLAPRAADERETQKHLWGISVQAGNQTSNYTSKYVLSEESKSPTRAKFPRQPSSEGTQVWSGDLMGCLEVSDSSDAPETTQKSSAIDTSKAAEEAMVLDPNYREQALQGLSEFKAATVSHCGPGAEEGEQGPGGAGGRQLEPKAGGEASRGRGALIIVAVEQKGLQASRSNAGPVPETLPRDFPKERPESPLSTGETPCESPTRGKTPAGNECELPAAPIQGAGDGALQRVAQAAELGRVLVPQAASEETPSTEEPPGERLRGESRSSGSGERGPEEAPEGGAAAARGQRPRVPALEPPQPPRGLRKGAQEPGKRPTFSKVTSFRKGRPLATESPGGVPAPTTEGRRWGSSGPEGLPRENPPAAAGRDAPPLHHGDASAWPEFVPQAAGDRTAGPAGAGHTGTSGDLGSRGPSSESCNAKRLKTTEKKLRARLALAHKTFSNFIESIVLEKENTHERSPSSPKGEKEKSRLRQGSWRAFLKSKDAGSPKKPTLVSLPLGPEVLSPAETDSHCEERAEDKEGYVFSDHWAPPLASTPLSSSLVSPEHRRKSEPTIKCTATQEGGRYLPSGIFPEKSWLASPGSPRAQQAGIAHTLPSSSACCLAYENPGTPCRPTSPKPLSPRPSAQRMGLHYPGRGSAISMVSLGSYSYVDSSSGDPERPKIPKGQTSFLLSLQTLNQDEQKEESREGGQGPRGLGTVPWLRDLPGSENHMPWEEPAGEKPSCSHSQKAFHMEPAQKPCFTTDMVTWALLCISAETVRGEAPSQPRGIPHRSPVSVDDLWLEKTQRKKLQKQAHVERRLHIGAVHKDGVKCWRKTIITSPESLNLPRRSHPLSQSAPTGLNHMGWPEHTPGTAMPDGALDTAVCADEVGSEEDLYDDLHSSSHHYSHPGGGGEQLAINELISDGSVVCAEALWDHVTMDDQELGFKAGDVIEVMDATNREWWWGRVADGEGWFPASFVRLRVNQDEPADDDAPLAGNSGAEDGGAEAQSSKDQMRTNVINEILSTERDYIKHLRDICEGYVRQCRKRADMFSEEQLRTIFGNIEDIYRCQKAFVKALEQRFNRERPHLSELGACFLEHQADFQIYSEYCNNHPNACVELSRLTKLSKYVYFFEACRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTHPQHRDFKDVEAALHAMKNVAQLINERKRRLENIDKIAQWQSSIEDWEGEDLLVRSSELIYSGELTRVTQPQAKSQQRMFFLFDHQLIYCKKDLLRRDVLYYKGRLDMDGLEVVDLEDGKDRDLHVSIKNAFRLHRGATGDSHLLCTRKPEQKQRWLKAFAREREQVQLDQETGFSITELQRKQAMLNASKQQVTGKPKAVGRPCYLTRQKHPALPSNRPQQQVLVLAEPRRKPSTFWHSISRLAPFRK

Target ORF information:

RefSeq Version	NM_001367493.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001367493.1

ORF Insert Sequence:

ATGCTCAGCG TCGTGCACTT CCTCCGGAGC TTCTTCAAGA CTCCAGAGCC AGGTGCACAC 
CTGCCAGGTG AAGGTGAGAT TGAAGATAAC CAGCTCCCCA CATCCCCTGC AGAACAAGTG 
GAGCAGGGAT GGAACCAGCA AACAGACCGC GATGATTCTG AAACGCTGTC CCAGCAGAGT 
GAAAGTGGAT CAGACACAAA AACTGACCCC TTTGAAAGTG CCTCTGACAC AGAGTCCTTG 
TCTGGGTACC TCCCGAGGGG AGTCTTCCAC CCTCTAAGAG GTACTCCCGT AGATATAGAA 
GCACCTTGGG AATACCCTGA TGTCTCAGCA ACTGGACCCC CTCAGGAGCA GCATTTGACC 
AGTGTTCCTG GGCTTCATGC AAAGGAAGAA CTCGATTTGT CCCCTAGCTT AGAGGATGAC 
TCTTGCAAAA ATGGGTGGCG AGCCTTTGCC ACAGTTGCTG GAGAACAGGA GGCAGGACAC 
CTCTGGGACT GCGCGACCAG CCTGGAGCGA GAGTCTTTGC TGGCAGGGGT TCCCCGACAC 
ACAGGGTGCT GCTTACAGAG GGCCACAGAC AGCAGTGGTC CTGAGCCAGT ACAGGGGGTG 
GCTGTTCAAG ACCTCAGAGG GCTCTCCAGT GTTTCTCTTC AGAAATCCAG GTCTGAGAGC 
TATCTGGGCA TCCCAGTGGT CTGGCCCTTC CTTCTCTGGT GCTGTGAACT GGGTCGGAGT 
TGGCCACATA TCCACAACAG GGCCAGGGCA CTGGTGCTAC CTAGCAGAGG CCCACTGGAC 
AACACAGCCC CTCTGGGGAC CAGGACAAAG AAGGAAAGTA CTCTAGGCCC TGCAGGGGAC 
ACAGAATTGC TCTGGTCCCA GCCCCACTCG GATGTCCCCT GCCAGCCTCC TTTGAGGACA 
TCTTGCCTCC TACGCACCAA CCGTCACCAT AGTGCCCCAG AAACTACTGG TGACAAGAAC 
AGGGCATCCC CCAGACTCAA CTGTGGGCAC ATGAGGGCAC TGGTCAGGGC CCATTCCATA 
GCAGGGTTTT CACCAGAGTG CCCCGAGGAT CCCGTGGGAC AGAATGTTGT GAAGTCTGGG 
ACCCATGTGA AGGAAGGGGC CAAAAATGAA CGAGATCCAA GAATACAAAA CATCCCTTCC 
CCTGCACCCA CCCAGCTGTC TGGCCCGATT CCTGCTTTTC AGAGTGGGGC TCCCCATCTG 
CAGGGTCCCT GCAAGCCTGG TGGGTTTCGC TTGCAAAGGG CCTCTCAGGA CACTCCTTCT 
GCAGGTCTCC TGGGGGAAAA CCAGTTAAGA CAGGATTCCA GGTCATGTCT GGTGGCTTCA 
TGCCTCACCT CAGAGTTAGT GAAGCTCAGT GCAGAGGAAG TGCCTGAGCC CGCTGAGTGC 
AAGTCAGAGC AAAGCCCAGA AAGCAGAACC CAGGAACCCC AAGGCACCCA ACTCGCACCA 
AGAGCTGCTG ATGAGAGAGA GACACAGAAG CACCTCTGGG GCATTTCTGT CCAGGCAGGA 
AACCAAACCA GTAATTACAC ATCAAAGTAT GTGCTCAGCG AGGAAAGCAA GTCACCTACC 
AGGGCCAAGT TCCCACGGCA GCCTAGCAGT GAGGGGACCC AGGTGTGGAG TGGAGACTTG 
ATGGGCTGCT TGGAAGTGAG TGACAGTTCA GATGCCCCTG AGACCACCCA GAAATCAAGC 
GCAATAGACA CTTCAAAGGC AGCCGAAGAA GCCATGGTTC TAGACCCCAA CTACAGGGAA 
CAGGCTTTGC AAGGTCTTTC AGAATTCAAG GCAGCCACGG TGTCTCACTG CGGCCCCGGG 
GCTGAGGAGG GTGAACAGGG GCCTGGGGGT GCCGGGGGCC GGCAGCTGGA GCCCAAAGCA 
GGCGGCGAGG CCTCGAGGGG CAGGGGCGCC CTCATCATTG TAGCTGTGGA GCAGAAAGGT 
CTTCAGGCCA GCAGGAGCAA TGCGGGGCCT GTTCCAGAAA CACTGCCCCG TGACTTTCCT 
AAGGAAAGAC CAGAATCTCC CTTGAGCACT GGCGAGACCC CCTGTGAGTC TCCCACTAGG 
GGGAAAACAC CAGCCGGTAA TGAGTGTGAG TTGCCAGCAG CCCCCATACA GGGAGCTGGC 
GATGGGGCTC TTCAGCGGGT GGCCCAGGCC GCAGAGCTTG GGAGAGTGCT GGTCCCCCAA 
GCTGCTTCGG AAGAGACGCC GAGCACAGAG GAGCCCCCGG GAGAGAGACT GCGTGGGGAG 
AGCCGGAGCT CCGGGTCAGG GGAGCGTGGC CCGGAGGAGG CCCCCGAAGG CGGTGCTGCA 
GCAGCCCGGG GCCAGCGCCC CCGCGTCCCC GCCTTGGAGC CGCCCCAGCC GCCACGCGGG 
CTCCGCAAGG GCGCGCAGGA GCCTGGGAAG CGCCCGACGT TTTCCAAGGT GACCTCCTTC 
AGGAAGGGCA GGCCCTTGGC CACTGAGAGC CCAGGAGGGG TCCCGGCCCC GACCACCGAG 
GGTCGCCGCT GGGGCTCTTC AGGCCCCGAG GGGCTCCCCA GGGAGAATCC GCCCGCTGCG 
GCCGGTCGGG ACGCACCGCC TCTGCACCAC GGGGACGCCA GCGCCTGGCC CGAGTTTGTC 
CCGCAGGCTG CAGGCGACAG GACTGCAGGG CCGGCAGGAG CGGGGCACAC GGGGACCTCA 
GGGGATCTTG GTAGCCGAGG GCCTTCCTCT GAGAGCTGCA ACGCAAAGAG ACTCAAAACA 
ACGGAGAAAA AACTCAGGGC AAGGTTGGCC TTGGCTCATA AGACCTTTTC AAACTTTATT 
GAGTCAATAG TTCTAGAGAA AGAGAACACC CATGAACGTT CCCCAAGTTC TCCCAAGGGC 
GAGAAGGAGA AGAGCAGGCT GCGCCAGGGT TCCTGGCGGG CGTTTCTGAA AAGCAAAGAT 
GCCGGAAGCC CCAAAAAGCC CACCCTAGTG AGTCTGCCTC TAGGACCCGA AGTTCTCTCC 
CCAGCAGAGA CCGACAGCCA CTGTGAGGAA CGGGCGGAGG ACAAAGAGGG CTATGTTTTT 
AGCGATCACT GGGCACCCCC ACTTGCCTCC ACACCTTTGT CCTCCAGTTT AGTTTCTCCA 
GAACACAGGA GGAAAAGTGA ACCGACCATC AAGTGCACAG CCACCCAGGA AGGCGGTAGG 
TACCTACCTT CAGGTATCTT TCCGGAAAAG TCCTGGCTGG CGTCCCCCGG CAGCCCTCGG 
GCCCAGCAGG CTGGAATCGC ACACACCCTG CCTTCCAGCT CTGCCTGCTG CCTGGCATAT 
GAAAACCCCG GGACGCCCTG CAGACCCACG AGCCCCAAGC CCCTGAGTCC CAGGCCTAGT 
GCTCAGCGGA TGGGTCTCCA CTACCCCGGG AGGGGTAGCG CCATCTCCAT GGTTTCTCTT 
GGAAGCTACA GCTACGTGGA CAGCAGTTCA GGGGACCCTG AAAGACCCAA GATTCCCAAG 
GGCCAGACCA GTTTCCTGCT TTCTCTGCAG ACGCTAAACC AAGATGAGCA GAAGGAAGAG 
AGCAGGGAAG GAGGCCAGGG TCCGCGCGGC TTGGGCACAG TGCCCTGGCT CAGGGACCTT 
CCTGGGAGTG AGAACCACAT GCCCTGGGAA GAACCAGCAG GTGAGAAGCC CAGTTGCTCT 
CACAGTCAGA AGGCGTTCCA CATGGAGCCT GCCCAGAAGC CCTGCTTCAC CACTGACATG 
GTGACATGGG CCCTCCTCTG CATCTCTGCA GAGACTGTGC GTGGGGAGGC TCCTTCACAG 
CCTAGGGGCA TCCCTCACCG CTCGCCCGTC AGTGTGGATG ACCTGTGGCT GGAGAAGACA 
CAGAGAAAGA AGTTGCAGAA GCAGGCCCAC GTCGAAAGGA GGCTGCACAT AGGGGCAGTG 
CACAAAGATG GAGTCAAGTG CTGGAGAAAG ACGATCATTA CCTCTCCAGA GTCTTTGAAT 
CTCCCTAGAA GAAGCCATCC ACTCTCCCAG AGTGCTCCAA CGGGACTGAA CCACATGGGC 
TGGCCAGAGC ACACACCAGG CACTGCCATG CCTGATGGAG CTCTGGACAC AGCTGTCTGC 
GCTGACGAAG TGGGGAGCGA GGAGGACCTG TATGATGACC TGCACAGCTC CAGCCACCAC 
TACAGCCACC CTGGAGGGGG TGGGGAGCAG CTGGCTATCA ATGAGCTCAT CAGCGATGGC 
AGTGTGGTCT GCGCTGAAGC ACTCTGGGAC CATGTCACCA TGGACGACCA GGAGCTGGGC 
TTCAAAGCTG GGGACGTCAT CGAAGTGATG GATGCCACCA ACAGAGAGTG GTGGTGGGGC 
CGGGTCGCCG ATGGCGAGGG CTGGTTTCCA GCCAGCTTCG TTCGGCTGAG GGTGAATCAG 
GACGAGCCCG CGGATGACGA CGCCCCTCTG GCCGGGAACA GCGGAGCGGA GGACGGCGGG 
GCGGAGGCGC AGAGCAGCAA GGACCAGATG CGGACCAACG TCATCAACGA GATCCTCAGC 
ACTGAGCGGG ACTACATCAA GCACCTGCGC GACATCTGCG AGGGCTACGT CCGGCAGTGC 
CGCAAGCGCG CAGACATGTT CAGCGAGGAG CAGCTGCGTA CCATCTTCGG GAACATCGAG 
GACATCTACC GCTGCCAGAA GGCCTTCGTG AAGGCCCTGG AGCAGAGGTT CAACCGCGAG 
CGCCCACACC TGAGCGAGCT GGGTGCCTGC TTCCTGGAGC ATCAAGCCGA CTTCCAGATC 
TACTCGGAGT ACTGCAATAA CCACCCCAAC GCCTGCGTGG AGCTCTCCCG GCTCACCAAG 
CTCAGCAAGT ACGTGTACTT CTTCGAGGCC TGCCGGCTGC TGCAGAAGAT GATTGACATC 
TCCCTGGATG GCTTCCTGCT GACTCCGGTG CAGAAGATCT GCAAGTACCC TCTGCAGCTG 
GCCGAGCTGC TCAAATACAC GCACCCCCAG CACAGGGACT TCAAGGATGT TGAAGCCGCC 
TTGCATGCCA TGAAGAACGT GGCCCAGCTC ATCAACGAGC GGAAGCGGAG ACTTGAGAAC 
ATCGACAAGA TTGCTCAGTG GCAGAGCTCC ATAGAGGACT GGGAGGGAGA AGATCTCTTG 
GTCAGGAGCT CAGAACTCAT CTACTCGGGG GAGCTGACTC GAGTTACACA GCCTCAAGCC 
AAAAGCCAGC AGCGAATGTT CTTTCTCTTT GACCACCAGC TCATCTACTG TAAGAAGGAC 
CTGCTCCGCC GCGACGTGTT GTACTACAAG GGCCGGCTGG ACATGGACGG CCTGGAGGTG 
GTGGACCTGG AGGACGGGAA GGACAGAGAC CTCCATGTGA GCATCAAGAA CGCCTTCCGG 
CTGCACCGTG GCGCCACAGG GGACAGCCAC CTGCTGTGCA CCAGGAAGCC CGAGCAGAAG 
CAGCGCTGGC TCAAGGCCTT TGCCAGGGAG AGGGAGCAGG TGCAGCTGGA CCAGGAGACA 
GGCTTCTCCA TCACTGAACT GCAGAGGAAG CAGGCCATGC TGAATGCCAG CAAGCAGCAG 
GTCACAGGGA AGCCCAAAGC TGTTGGCCGG CCCTGCTACC TGACGCGCCA GAAGCACCCA 
GCCCTGCCCA GCAACCGGCC CCAGCAGCAG GTCCTGGTGC TGGCGGAGCC CAGGCGCAAG 
CCATCTACCT TCTGGCACAG CATCAGCCGG CTGGCACCCT TCCGCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF4 ( XM_005263681.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005263738.1 Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu36328
Clone ID Related Accession (Same CDS sequence)	NM_001367493.1 , XM_005263681.1
Accession Version	XM_005263681.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5631bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAGCG TCGTGCACTT CCTCCGGAGC TTCTTCAAGA CTCCAGAGCC AGGTGCACAC 
CTGCCAGGTG AAGGTGAGAT TGAAGATAAC CAGCTCCCCA CATCCCCTGC AGAACAAGTG 
GAGCAGGGAT GGAACCAGCA AACAGACCGC GATGATTCTG AAACGCTGTC CCAGCAGAGT 
GAAAGTGGAT CAGACACAAA AACTGACCCC TTTGAAAGTG CCTCTGACAC AGAGTCCTTG 
TCTGGGTACC TCCCGAGGGG AGTCTTCCAC CCTCTAAGAG GTACTCCCGT AGATATAGAA 
GCACCTTGGG AATACCCTGA TGTCTCAGCA ACTGGACCCC CTCAGGAGCA GCATTTGACC 
AGTGTTCCTG GGCTTCATGC AAAGGAAGAA CTCGATTTGT CCCCTAGCTT AGAGGATGAC 
TCTTGCAAAA ATGGGTGGCG AGCCTTTGCC ACAGTTGCTG GAGAACAGGA GGCAGGACAC 
CTCTGGGACT GCGCGACCAG CCTGGAGCGA GAGTCTTTGC TGGCAGGGGT TCCCCGACAC 
ACAGGGTGCT GCTTACAGAG GGCCACAGAC AGCAGTGGTC CTGAGCCAGT ACAGGGGGTG 
GCTGTTCAAG ACCTCAGAGG GCTCTCCAGT GTTTCTCTTC AGAAATCCAG GTCTGAGAGC 
TATCTGGGCA TCCCAGTGGT CTGGCCCTTC CTTCTCTGGT GCTGTGAACT GGGTCGGAGT 
TGGCCACATA TCCACAACAG GGCCAGGGCA CTGGTGCTAC CTAGCAGAGG CCCACTGGAC 
AACACAGCCC CTCTGGGGAC CAGGACAAAG AAGGAAAGTA CTCTAGGCCC TGCAGGGGAC 
ACAGAATTGC TCTGGTCCCA GCCCCACTCG GATGTCCCCT GCCAGCCTCC TTTGAGGACA 
TCTTGCCTCC TACGCACCAA CCGTCACCAT AGTGCCCCAG AAACTACTGG TGACAAGAAC 
AGGGCATCCC CCAGACTCAA CTGTGGGCAC ATGAGGGCAC TGGTCAGGGC CCATTCCATA 
GCAGGGTTTT CACCAGAGTG CCCCGAGGAT CCCGTGGGAC AGAATGTTGT GAAGTCTGGG 
ACCCATGTGA AGGAAGGGGC CAAAAATGAA CGAGATCCAA GAATACAAAA CATCCCTTCC 
CCTGCACCCA CCCAGCTGTC TGGCCCGATT CCTGCTTTTC AGAGTGGGGC TCCCCATCTG 
CAGGGTCCCT GCAAGCCTGG TGGGTTTCGC TTGCAAAGGG CCTCTCAGGA CACTCCTTCT 
GCAGGTCTCC TGGGGGAAAA CCAGTTAAGA CAGGATTCCA GGTCATGTCT GGTGGCTTCA 
TGCCTCACCT CAGAGTTAGT GAAGCTCAGT GCAGAGGAAG TGCCTGAGCC CGCTGAGTGC 
AAGTCAGAGC AAAGCCCAGA AAGCAGAACC CAGGAACCCC AAGGCACCCA ACTCGCACCA 
AGAGCTGCTG ATGAGAGAGA GACACAGAAG CACCTCTGGG GCATTTCTGT CCAGGCAGGA 
AACCAAACCA GTAATTACAC ATCAAAGTAT GTGCTCAGCG AGGAAAGCAA GTCACCTACC 
AGGGCCAAGT TCCCACGGCA GCCTAGCAGT GAGGGGACCC AGGTGTGGAG TGGAGACTTG 
ATGGGCTGCT TGGAAGTGAG TGACAGTTCA GATGCCCCTG AGACCACCCA GAAATCAAGC 
GCAATAGACA CTTCAAAGGC AGCCGAAGAA GCCATGGTTC TAGACCCCAA CTACAGGGAA 
CAGGCTTTGC AAGGTCTTTC AGAATTCAAG GCAGCCACGG TGTCTCACTG CGGCCCCGGG 
GCTGAGGAGG GTGAACAGGG GCCTGGGGGT GCCGGGGGCC GGCAGCTGGA GCCCAAAGCA 
GGCGGCGAGG CCTCGAGGGG CAGGGGCGCC CTCATCATTG TAGCTGTGGA GCAGAAAGGT 
CTTCAGGCCA GCAGGAGCAA TGCGGGGCCT GTTCCAGAAA CACTGCCCCG TGACTTTCCT 
AAGGAAAGAC CAGAATCTCC CTTGAGCACT GGCGAGACCC CCTGTGAGTC TCCCACTAGG 
GGGAAAACAC CAGCCGGTAA TGAGTGTGAG TTGCCAGCAG CCCCCATACA GGGAGCTGGC 
GATGGGGCTC TTCAGCGGGT GGCCCAGGCC GCAGAGCTTG GGAGAGTGCT GGTCCCCCAA 
GCTGCTTCGG AAGAGACGCC GAGCACAGAG GAGCCCCCGG GAGAGAGACT GCGTGGGGAG 
AGCCGGAGCT CCGGGTCAGG GGAGCGTGGC CCGGAGGAGG CCCCCGAAGG CGGTGCTGCA 
GCAGCCCGGG GCCAGCGCCC CCGCGTCCCC GCCTTGGAGC CGCCCCAGCC GCCACGCGGG 
CTCCGCAAGG GCGCGCAGGA GCCTGGGAAG CGCCCGACGT TTTCCAAGGT GACCTCCTTC 
AGGAAGGGCA GGCCCTTGGC CACTGAGAGC CCAGGAGGGG TCCCGGCCCC GACCACCGAG 
GGTCGCCGCT GGGGCTCTTC AGGCCCCGAG GGGCTCCCCA GGGAGAATCC GCCCGCTGCG 
GCCGGTCGGG ACGCACCGCC TCTGCACCAC GGGGACGCCA GCGCCTGGCC CGAGTTTGTC 
CCGCAGGCTG CAGGCGACAG GACTGCAGGG CCGGCAGGAG CGGGGCACAC GGGGACCTCA 
GGGGATCTTG GTAGCCGAGG GCCTTCCTCT GAGAGCTGCA ACGCAAAGAG ACTCAAAACA 
ACGGAGAAAA AACTCAGGGC AAGGTTGGCC TTGGCTCATA AGACCTTTTC AAACTTTATT 
GAGTCAATAG TTCTAGAGAA AGAGAACACC CATGAACGTT CCCCAAGTTC TCCCAAGGGC 
GAGAAGGAGA AGAGCAGGCT GCGCCAGGGT TCCTGGCGGG CGTTTCTGAA AAGCAAAGAT 
GCCGGAAGCC CCAAAAAGCC CACCCTAGTG AGTCTGCCTC TAGGACCCGA AGTTCTCTCC 
CCAGCAGAGA CCGACAGCCA CTGTGAGGAA CGGGCGGAGG ACAAAGAGGG CTATGTTTTT 
AGCGATCACT GGGCACCCCC ACTTGCCTCC ACACCTTTGT CCTCCAGTTT AGTTTCTCCA 
GAACACAGGA GGAAAAGTGA ACCGACCATC AAGTGCACAG CCACCCAGGA AGGCGGTAGG 
TACCTACCTT CAGGTATCTT TCCGGAAAAG TCCTGGCTGG CGTCCCCCGG CAGCCCTCGG 
GCCCAGCAGG CTGGAATCGC ACACACCCTG CCTTCCAGCT CTGCCTGCTG CCTGGCATAT 
GAAAACCCCG GGACGCCCTG CAGACCCACG AGCCCCAAGC CCCTGAGTCC CAGGCCTAGT 
GCTCAGCGGA TGGGTCTCCA CTACCCCGGG AGGGGTAGCG CCATCTCCAT GGTTTCTCTT 
GGAAGCTACA GCTACGTGGA CAGCAGTTCA GGGGACCCTG AAAGACCCAA GATTCCCAAG 
GGCCAGACCA GTTTCCTGCT TTCTCTGCAG ACGCTAAACC AAGATGAGCA GAAGGAAGAG 
AGCAGGGAAG GAGGCCAGGG TCCGCGCGGC TTGGGCACAG TGCCCTGGCT CAGGGACCTT 
CCTGGGAGTG AGAACCACAT GCCCTGGGAA GAACCAGCAG GTGAGAAGCC CAGTTGCTCT 
CACAGTCAGA AGGCGTTCCA CATGGAGCCT GCCCAGAAGC CCTGCTTCAC CACTGACATG 
GTGACATGGG CCCTCCTCTG CATCTCTGCA GAGACTGTGC GTGGGGAGGC TCCTTCACAG 
CCTAGGGGCA TCCCTCACCG CTCGCCCGTC AGTGTGGATG ACCTGTGGCT GGAGAAGACA 
CAGAGAAAGA AGTTGCAGAA GCAGGCCCAC GTCGAAAGGA GGCTGCACAT AGGGGCAGTG 
CACAAAGATG GAGTCAAGTG CTGGAGAAAG ACGATCATTA CCTCTCCAGA GTCTTTGAAT 
CTCCCTAGAA GAAGCCATCC ACTCTCCCAG AGTGCTCCAA CGGGACTGAA CCACATGGGC 
TGGCCAGAGC ACACACCAGG CACTGCCATG CCTGATGGAG CTCTGGACAC AGCTGTCTGC 
GCTGACGAAG TGGGGAGCGA GGAGGACCTG TATGATGACC TGCACAGCTC CAGCCACCAC 
TACAGCCACC CTGGAGGGGG TGGGGAGCAG CTGGCTATCA ATGAGCTCAT CAGCGATGGC 
AGTGTGGTCT GCGCTGAAGC ACTCTGGGAC CATGTCACCA TGGACGACCA GGAGCTGGGC 
TTCAAAGCTG GGGACGTCAT CGAAGTGATG GATGCCACCA ACAGAGAGTG GTGGTGGGGC 
CGGGTCGCCG ATGGCGAGGG CTGGTTTCCA GCCAGCTTCG TTCGGCTGAG GGTGAATCAG 
GACGAGCCCG CGGATGACGA CGCCCCTCTG GCCGGGAACA GCGGAGCGGA GGACGGCGGG 
GCGGAGGCGC AGAGCAGCAA GGACCAGATG CGGACCAACG TCATCAACGA GATCCTCAGC 
ACTGAGCGGG ACTACATCAA GCACCTGCGC GACATCTGCG AGGGCTACGT CCGGCAGTGC 
CGCAAGCGCG CAGACATGTT CAGCGAGGAG CAGCTGCGTA CCATCTTCGG GAACATCGAG 
GACATCTACC GCTGCCAGAA GGCCTTCGTG AAGGCCCTGG AGCAGAGGTT CAACCGCGAG 
CGCCCACACC TGAGCGAGCT GGGTGCCTGC TTCCTGGAGC ATCAAGCCGA CTTCCAGATC 
TACTCGGAGT ACTGCAATAA CCACCCCAAC GCCTGCGTGG AGCTCTCCCG GCTCACCAAG 
CTCAGCAAGT ACGTGTACTT CTTCGAGGCC TGCCGGCTGC TGCAGAAGAT GATTGACATC 
TCCCTGGATG GCTTCCTGCT GACTCCGGTG CAGAAGATCT GCAAGTACCC TCTGCAGCTG 
GCCGAGCTGC TCAAATACAC GCACCCCCAG CACAGGGACT TCAAGGATGT TGAAGCCGCC 
TTGCATGCCA TGAAGAACGT GGCCCAGCTC ATCAACGAGC GGAAGCGGAG ACTTGAGAAC 
ATCGACAAGA TTGCTCAGTG GCAGAGCTCC ATAGAGGACT GGGAGGGAGA AGATCTCTTG 
GTCAGGAGCT CAGAACTCAT CTACTCGGGG GAGCTGACTC GAGTTACACA GCCTCAAGCC 
AAAAGCCAGC AGCGAATGTT CTTTCTCTTT GACCACCAGC TCATCTACTG TAAGAAGGAC 
CTGCTCCGCC GCGACGTGTT GTACTACAAG GGCCGGCTGG ACATGGACGG CCTGGAGGTG 
GTGGACCTGG AGGACGGGAA GGACAGAGAC CTCCATGTGA GCATCAAGAA CGCCTTCCGG 
CTGCACCGTG GCGCCACAGG GGACAGCCAC CTGCTGTGCA CCAGGAAGCC CGAGCAGAAG 
CAGCGCTGGC TCAAGGCCTT TGCCAGGGAG AGGGAGCAGG TGCAGCTGGA CCAGGAGACA 
GGCTTCTCCA TCACTGAACT GCAGAGGAAG CAGGCCATGC TGAATGCCAG CAAGCAGCAG 
GTCACAGGGA AGCCCAAAGC TGTTGGCCGG CCCTGCTACC TGACGCGCCA GAAGCACCCA 
GCCCTGCCCA GCAACCGGCC CCAGCAGCAG GTCCTGGTGC TGGCGGAGCC CAGGCGCAAG 
CCATCTACCT TCTGGCACAG CATCAGCCGG CTGGCACCCT TCCGCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005263738.1
CDS	39..5669
Translation	MLSVVHFLRSFFKTPEPGAHLPGEGEIEDNQLPTSPAEQVEQGWNQQTDRDDSETLSQQSESGSDTKTDPFESASDTESLSGYLPRGVFHPLRGTPVDIEAPWEYPDVSATGPPQEQHLTSVPGLHAKEELDLSPSLEDDSCKNGWRAFATVAGEQEAGHLWDCATSLERESLLAGVPRHTGCCLQRATDSSGPEPVQGVAVQDLRGLSSVSLQKSRSESYLGIPVVWPFLLWCCELGRSWPHIHNRARALVLPSRGPLDNTAPLGTRTKKESTLGPAGDTELLWSQPHSDVPCQPPLRTSCLLRTNRHHSAPETTGDKNRASPRLNCGHMRALVRAHSIAGFSPECPEDPVGQNVVKSGTHVKEGAKNERDPRIQNIPSPAPTQLSGPIPAFQSGAPHLQGPCKPGGFRLQRASQDTPSAGLLGENQLRQDSRSCLVASCLTSELVKLSAEEVPEPAECKSEQSPESRTQEPQGTQLAPRAADERETQKHLWGISVQAGNQTSNYTSKYVLSEESKSPTRAKFPRQPSSEGTQVWSGDLMGCLEVSDSSDAPETTQKSSAIDTSKAAEEAMVLDPNYREQALQGLSEFKAATVSHCGPGAEEGEQGPGGAGGRQLEPKAGGEASRGRGALIIVAVEQKGLQASRSNAGPVPETLPRDFPKERPESPLSTGETPCESPTRGKTPAGNECELPAAPIQGAGDGALQRVAQAAELGRVLVPQAASEETPSTEEPPGERLRGESRSSGSGERGPEEAPEGGAAAARGQRPRVPALEPPQPPRGLRKGAQEPGKRPTFSKVTSFRKGRPLATESPGGVPAPTTEGRRWGSSGPEGLPRENPPAAAGRDAPPLHHGDASAWPEFVPQAAGDRTAGPAGAGHTGTSGDLGSRGPSSESCNAKRLKTTEKKLRARLALAHKTFSNFIESIVLEKENTHERSPSSPKGEKEKSRLRQGSWRAFLKSKDAGSPKKPTLVSLPLGPEVLSPAETDSHCEERAEDKEGYVFSDHWAPPLASTPLSSSLVSPEHRRKSEPTIKCTATQEGGRYLPSGIFPEKSWLASPGSPRAQQAGIAHTLPSSSACCLAYENPGTPCRPTSPKPLSPRPSAQRMGLHYPGRGSAISMVSLGSYSYVDSSSGDPERPKIPKGQTSFLLSLQTLNQDEQKEESREGGQGPRGLGTVPWLRDLPGSENHMPWEEPAGEKPSCSHSQKAFHMEPAQKPCFTTDMVTWALLCISAETVRGEAPSQPRGIPHRSPVSVDDLWLEKTQRKKLQKQAHVERRLHIGAVHKDGVKCWRKTIITSPESLNLPRRSHPLSQSAPTGLNHMGWPEHTPGTAMPDGALDTAVCADEVGSEEDLYDDLHSSSHHYSHPGGGGEQLAINELISDGSVVCAEALWDHVTMDDQELGFKAGDVIEVMDATNREWWWGRVADGEGWFPASFVRLRVNQDEPADDDAPLAGNSGAEDGGAEAQSSKDQMRTNVINEILSTERDYIKHLRDICEGYVRQCRKRADMFSEEQLRTIFGNIEDIYRCQKAFVKALEQRFNRERPHLSELGACFLEHQADFQIYSEYCNNHPNACVELSRLTKLSKYVYFFEACRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTHPQHRDFKDVEAALHAMKNVAQLINERKRRLENIDKIAQWQSSIEDWEGEDLLVRSSELIYSGELTRVTQPQAKSQQRMFFLFDHQLIYCKKDLLRRDVLYYKGRLDMDGLEVVDLEDGKDRDLHVSIKNAFRLHRGATGDSHLLCTRKPEQKQRWLKAFAREREQVQLDQETGFSITELQRKQAMLNASKQQVTGKPKAVGRPCYLTRQKHPALPSNRPQQQVLVLAEPRRKPSTFWHSISRLAPFRK

Target ORF information:

RefSeq Version	XM_005263681.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005263681.1

ORF Insert Sequence:

ATGCTCAGCG TCGTGCACTT CCTCCGGAGC TTCTTCAAGA CTCCAGAGCC AGGTGCACAC 
CTGCCAGGTG AAGGTGAGAT TGAAGATAAC CAGCTCCCCA CATCCCCTGC AGAACAAGTG 
GAGCAGGGAT GGAACCAGCA AACAGACCGC GATGATTCTG AAACGCTGTC CCAGCAGAGT 
GAAAGTGGAT CAGACACAAA AACTGACCCC TTTGAAAGTG CCTCTGACAC AGAGTCCTTG 
TCTGGGTACC TCCCGAGGGG AGTCTTCCAC CCTCTAAGAG GTACTCCCGT AGATATAGAA 
GCACCTTGGG AATACCCTGA TGTCTCAGCA ACTGGACCCC CTCAGGAGCA GCATTTGACC 
AGTGTTCCTG GGCTTCATGC AAAGGAAGAA CTCGATTTGT CCCCTAGCTT AGAGGATGAC 
TCTTGCAAAA ATGGGTGGCG AGCCTTTGCC ACAGTTGCTG GAGAACAGGA GGCAGGACAC 
CTCTGGGACT GCGCGACCAG CCTGGAGCGA GAGTCTTTGC TGGCAGGGGT TCCCCGACAC 
ACAGGGTGCT GCTTACAGAG GGCCACAGAC AGCAGTGGTC CTGAGCCAGT ACAGGGGGTG 
GCTGTTCAAG ACCTCAGAGG GCTCTCCAGT GTTTCTCTTC AGAAATCCAG GTCTGAGAGC 
TATCTGGGCA TCCCAGTGGT CTGGCCCTTC CTTCTCTGGT GCTGTGAACT GGGTCGGAGT 
TGGCCACATA TCCACAACAG GGCCAGGGCA CTGGTGCTAC CTAGCAGAGG CCCACTGGAC 
AACACAGCCC CTCTGGGGAC CAGGACAAAG AAGGAAAGTA CTCTAGGCCC TGCAGGGGAC 
ACAGAATTGC TCTGGTCCCA GCCCCACTCG GATGTCCCCT GCCAGCCTCC TTTGAGGACA 
TCTTGCCTCC TACGCACCAA CCGTCACCAT AGTGCCCCAG AAACTACTGG TGACAAGAAC 
AGGGCATCCC CCAGACTCAA CTGTGGGCAC ATGAGGGCAC TGGTCAGGGC CCATTCCATA 
GCAGGGTTTT CACCAGAGTG CCCCGAGGAT CCCGTGGGAC AGAATGTTGT GAAGTCTGGG 
ACCCATGTGA AGGAAGGGGC CAAAAATGAA CGAGATCCAA GAATACAAAA CATCCCTTCC 
CCTGCACCCA CCCAGCTGTC TGGCCCGATT CCTGCTTTTC AGAGTGGGGC TCCCCATCTG 
CAGGGTCCCT GCAAGCCTGG TGGGTTTCGC TTGCAAAGGG CCTCTCAGGA CACTCCTTCT 
GCAGGTCTCC TGGGGGAAAA CCAGTTAAGA CAGGATTCCA GGTCATGTCT GGTGGCTTCA 
TGCCTCACCT CAGAGTTAGT GAAGCTCAGT GCAGAGGAAG TGCCTGAGCC CGCTGAGTGC 
AAGTCAGAGC AAAGCCCAGA AAGCAGAACC CAGGAACCCC AAGGCACCCA ACTCGCACCA 
AGAGCTGCTG ATGAGAGAGA GACACAGAAG CACCTCTGGG GCATTTCTGT CCAGGCAGGA 
AACCAAACCA GTAATTACAC ATCAAAGTAT GTGCTCAGCG AGGAAAGCAA GTCACCTACC 
AGGGCCAAGT TCCCACGGCA GCCTAGCAGT GAGGGGACCC AGGTGTGGAG TGGAGACTTG 
ATGGGCTGCT TGGAAGTGAG TGACAGTTCA GATGCCCCTG AGACCACCCA GAAATCAAGC 
GCAATAGACA CTTCAAAGGC AGCCGAAGAA GCCATGGTTC TAGACCCCAA CTACAGGGAA 
CAGGCTTTGC AAGGTCTTTC AGAATTCAAG GCAGCCACGG TGTCTCACTG CGGCCCCGGG 
GCTGAGGAGG GTGAACAGGG GCCTGGGGGT GCCGGGGGCC GGCAGCTGGA GCCCAAAGCA 
GGCGGCGAGG CCTCGAGGGG CAGGGGCGCC CTCATCATTG TAGCTGTGGA GCAGAAAGGT 
CTTCAGGCCA GCAGGAGCAA TGCGGGGCCT GTTCCAGAAA CACTGCCCCG TGACTTTCCT 
AAGGAAAGAC CAGAATCTCC CTTGAGCACT GGCGAGACCC CCTGTGAGTC TCCCACTAGG 
GGGAAAACAC CAGCCGGTAA TGAGTGTGAG TTGCCAGCAG CCCCCATACA GGGAGCTGGC 
GATGGGGCTC TTCAGCGGGT GGCCCAGGCC GCAGAGCTTG GGAGAGTGCT GGTCCCCCAA 
GCTGCTTCGG AAGAGACGCC GAGCACAGAG GAGCCCCCGG GAGAGAGACT GCGTGGGGAG 
AGCCGGAGCT CCGGGTCAGG GGAGCGTGGC CCGGAGGAGG CCCCCGAAGG CGGTGCTGCA 
GCAGCCCGGG GCCAGCGCCC CCGCGTCCCC GCCTTGGAGC CGCCCCAGCC GCCACGCGGG 
CTCCGCAAGG GCGCGCAGGA GCCTGGGAAG CGCCCGACGT TTTCCAAGGT GACCTCCTTC 
AGGAAGGGCA GGCCCTTGGC CACTGAGAGC CCAGGAGGGG TCCCGGCCCC GACCACCGAG 
GGTCGCCGCT GGGGCTCTTC AGGCCCCGAG GGGCTCCCCA GGGAGAATCC GCCCGCTGCG 
GCCGGTCGGG ACGCACCGCC TCTGCACCAC GGGGACGCCA GCGCCTGGCC CGAGTTTGTC 
CCGCAGGCTG CAGGCGACAG GACTGCAGGG CCGGCAGGAG CGGGGCACAC GGGGACCTCA 
GGGGATCTTG GTAGCCGAGG GCCTTCCTCT GAGAGCTGCA ACGCAAAGAG ACTCAAAACA 
ACGGAGAAAA AACTCAGGGC AAGGTTGGCC TTGGCTCATA AGACCTTTTC AAACTTTATT 
GAGTCAATAG TTCTAGAGAA AGAGAACACC CATGAACGTT CCCCAAGTTC TCCCAAGGGC 
GAGAAGGAGA AGAGCAGGCT GCGCCAGGGT TCCTGGCGGG CGTTTCTGAA AAGCAAAGAT 
GCCGGAAGCC CCAAAAAGCC CACCCTAGTG AGTCTGCCTC TAGGACCCGA AGTTCTCTCC 
CCAGCAGAGA CCGACAGCCA CTGTGAGGAA CGGGCGGAGG ACAAAGAGGG CTATGTTTTT 
AGCGATCACT GGGCACCCCC ACTTGCCTCC ACACCTTTGT CCTCCAGTTT AGTTTCTCCA 
GAACACAGGA GGAAAAGTGA ACCGACCATC AAGTGCACAG CCACCCAGGA AGGCGGTAGG 
TACCTACCTT CAGGTATCTT TCCGGAAAAG TCCTGGCTGG CGTCCCCCGG CAGCCCTCGG 
GCCCAGCAGG CTGGAATCGC ACACACCCTG CCTTCCAGCT CTGCCTGCTG CCTGGCATAT 
GAAAACCCCG GGACGCCCTG CAGACCCACG AGCCCCAAGC CCCTGAGTCC CAGGCCTAGT 
GCTCAGCGGA TGGGTCTCCA CTACCCCGGG AGGGGTAGCG CCATCTCCAT GGTTTCTCTT 
GGAAGCTACA GCTACGTGGA CAGCAGTTCA GGGGACCCTG AAAGACCCAA GATTCCCAAG 
GGCCAGACCA GTTTCCTGCT TTCTCTGCAG ACGCTAAACC AAGATGAGCA GAAGGAAGAG 
AGCAGGGAAG GAGGCCAGGG TCCGCGCGGC TTGGGCACAG TGCCCTGGCT CAGGGACCTT 
CCTGGGAGTG AGAACCACAT GCCCTGGGAA GAACCAGCAG GTGAGAAGCC CAGTTGCTCT 
CACAGTCAGA AGGCGTTCCA CATGGAGCCT GCCCAGAAGC CCTGCTTCAC CACTGACATG 
GTGACATGGG CCCTCCTCTG CATCTCTGCA GAGACTGTGC GTGGGGAGGC TCCTTCACAG 
CCTAGGGGCA TCCCTCACCG CTCGCCCGTC AGTGTGGATG ACCTGTGGCT GGAGAAGACA 
CAGAGAAAGA AGTTGCAGAA GCAGGCCCAC GTCGAAAGGA GGCTGCACAT AGGGGCAGTG 
CACAAAGATG GAGTCAAGTG CTGGAGAAAG ACGATCATTA CCTCTCCAGA GTCTTTGAAT 
CTCCCTAGAA GAAGCCATCC ACTCTCCCAG AGTGCTCCAA CGGGACTGAA CCACATGGGC 
TGGCCAGAGC ACACACCAGG CACTGCCATG CCTGATGGAG CTCTGGACAC AGCTGTCTGC 
GCTGACGAAG TGGGGAGCGA GGAGGACCTG TATGATGACC TGCACAGCTC CAGCCACCAC 
TACAGCCACC CTGGAGGGGG TGGGGAGCAG CTGGCTATCA ATGAGCTCAT CAGCGATGGC 
AGTGTGGTCT GCGCTGAAGC ACTCTGGGAC CATGTCACCA TGGACGACCA GGAGCTGGGC 
TTCAAAGCTG GGGACGTCAT CGAAGTGATG GATGCCACCA ACAGAGAGTG GTGGTGGGGC 
CGGGTCGCCG ATGGCGAGGG CTGGTTTCCA GCCAGCTTCG TTCGGCTGAG GGTGAATCAG 
GACGAGCCCG CGGATGACGA CGCCCCTCTG GCCGGGAACA GCGGAGCGGA GGACGGCGGG 
GCGGAGGCGC AGAGCAGCAA GGACCAGATG CGGACCAACG TCATCAACGA GATCCTCAGC 
ACTGAGCGGG ACTACATCAA GCACCTGCGC GACATCTGCG AGGGCTACGT CCGGCAGTGC 
CGCAAGCGCG CAGACATGTT CAGCGAGGAG CAGCTGCGTA CCATCTTCGG GAACATCGAG 
GACATCTACC GCTGCCAGAA GGCCTTCGTG AAGGCCCTGG AGCAGAGGTT CAACCGCGAG 
CGCCCACACC TGAGCGAGCT GGGTGCCTGC TTCCTGGAGC ATCAAGCCGA CTTCCAGATC 
TACTCGGAGT ACTGCAATAA CCACCCCAAC GCCTGCGTGG AGCTCTCCCG GCTCACCAAG 
CTCAGCAAGT ACGTGTACTT CTTCGAGGCC TGCCGGCTGC TGCAGAAGAT GATTGACATC 
TCCCTGGATG GCTTCCTGCT GACTCCGGTG CAGAAGATCT GCAAGTACCC TCTGCAGCTG 
GCCGAGCTGC TCAAATACAC GCACCCCCAG CACAGGGACT TCAAGGATGT TGAAGCCGCC 
TTGCATGCCA TGAAGAACGT GGCCCAGCTC ATCAACGAGC GGAAGCGGAG ACTTGAGAAC 
ATCGACAAGA TTGCTCAGTG GCAGAGCTCC ATAGAGGACT GGGAGGGAGA AGATCTCTTG 
GTCAGGAGCT CAGAACTCAT CTACTCGGGG GAGCTGACTC GAGTTACACA GCCTCAAGCC 
AAAAGCCAGC AGCGAATGTT CTTTCTCTTT GACCACCAGC TCATCTACTG TAAGAAGGAC 
CTGCTCCGCC GCGACGTGTT GTACTACAAG GGCCGGCTGG ACATGGACGG CCTGGAGGTG 
GTGGACCTGG AGGACGGGAA GGACAGAGAC CTCCATGTGA GCATCAAGAA CGCCTTCCGG 
CTGCACCGTG GCGCCACAGG GGACAGCCAC CTGCTGTGCA CCAGGAAGCC CGAGCAGAAG 
CAGCGCTGGC TCAAGGCCTT TGCCAGGGAG AGGGAGCAGG TGCAGCTGGA CCAGGAGACA 
GGCTTCTCCA TCACTGAACT GCAGAGGAAG CAGGCCATGC TGAATGCCAG CAAGCAGCAG 
GTCACAGGGA AGCCCAAAGC TGTTGGCCGG CCCTGCTACC TGACGCGCCA GAAGCACCCA 
GCCCTGCCCA GCAACCGGCC CCAGCAGCAG GTCCTGGTGC TGGCGGAGCC CAGGCGCAAG 
CCATCTACCT TCTGGCACAG CATCAGCCGG CTGGCACCCT TCCGCAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF4 ( XM_005263685.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005263742.1 Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu36331
Clone ID Related Accession (Same CDS sequence)	XM_005263685.3
Accession Version	XM_005263685.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005263685.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAGCG TCGTGCACTT CCTCCGGAGC TTCTTCAAGA CTCCAGAGCC AGGTGCACAC 
CTGCCAGGTG AAGGTGAGAT TGAAGATAAC CAGCTCCCCA CATCCCCTGC AGAACAAGTG 
GAGCAGGGAT GGAACCAGCA AACAGACCGC GATGATTCTG AAACGCTGTC CCAGCAGAGT 
GAAAGTGGAT CAGACACAAA AACTGACCCC TTTGAAAGTG CCTCTGACAC AGAGTCCTTG 
TCTGGGTACC TCCCGAGGGG AGTCTTCCAC CCTCTAAGAG GTACTCCCGT AGATATAGAA 
GCACCTTGGG AATACCCTGA TGTCTCAGCA ACTGGACCCC CTCAGGAGCA GCATTTGACC 
AGTGTTCCTG GGCTTCATGC AAAGGAAGAA CTCGATTTGT CCCCTAGCTT AGAGGATGAC 
TCTTGCAAAA ATGGGTGGCG AGCCTTTGCC ACAGTTGCTG GAGAACAGGA GGCAGGACAC 
CTCTGGGACT GCGCGACCAG CCTGGAGCGA GAGTCTTTGC TGGCAGGGGT TCCCCGACAC 
ACAGGGTGCT GCTTACAGAG GGCCACAGAC AGCAGTGGTC CTGAGCCAGT ACAGGGGGTG 
GCTGTTCAAG ACCTCAGAGG GCTCTCCAGT GTTTCTCTTC AGAAATCCAG GTCTGAGAGC 
TATCTGGGCA TCCCAGTGGT CTGGCCCTTC CTTCTCTGGT GCTGTGAACT GGGTCGGAGT 
TGGCCACATA TCCACAACAG GGCCAGGGCA CTGGTGCTAC CTAGCAGAGG CCCACTGGAC 
AACACAGCCC CTCTGGGGAC CAGGACAAAG AAGGAAAGTA CTCTAGGCCC TGCAGGGGAC 
ACAGAATTGC TCTGGTCCCA GCCCCACTCG GATGTCCCCT GCCAGCCTCC TTTGAGGACA 
TCTTGCCTCC TACGCACCAA CCGTCACCAT AGTGCCCCAG AAACTACTGG TGACAAGAAC 
AGGGCATCCC CCAGACTCAA CTGTGGGCAC ATGAGGGCAC TGGTCAGGGC CCATTCCATA 
GCAGGGTTTT CACCAGAGTG CCCCGAGGAT CCCGTGGGAC AGAATGTTGT GAAGTCTGGG 
ACCCATGTGA AGGAAGGGGC CAAAAATGAA CGAGATCCAA GAATACAAAA CATCCCTTCC 
CCTGCACCCA CCCAGCTGTC TGGCCCGATT CCTGCTTTTC AGAGTGGGGC TCCCCATCTG 
CAGGGTCCCT GCAAGCCTGG TGGGTTTCGC TTGCAAAGGG CCTCTCAGGA CACTCCTTCT 
GCAGGTCTCC TGGGGGAAAA CCAGTTAAGA CAGGATTCCA GGTCATGTCT GGTGGCTTCA 
TGCCTCACCT CAGAGTTAGT GAAGCTCAGT GCAGAGGAAG TGCCTGAGCC CGCTGAGTGC 
AAGTCAGAGC AAAGCCCAGA AAGCAGAACC CAGGAACCCC AAGGCACCCA ACTCGCACCA 
AGAGCTGCTG ATGAGAGAGA GACACAGAAG CACCTCTGGG GCATTTCTGT CCAGGCAGGA 
AACCAAACCA GTAATTACAC ATCAAAGTAT GTGCTCAGCG AGGAAAGCAA GTCACCTACC 
AGGGCCAAGT TCCCACGGCA GCCTAGCAGT GAGGGGACCC AGGTGTGGAG TGGAGACTTG 
ATGGGCTGCT TGGAAGTGAG TGACAGTTCA GATGCCCCTG AGACCACCCA GAAATCAAGC 
GCAATAGACA CTTCAAAGGC AGCCGAAGAA GCCATGGTTC TAGACCCCAA CTACAGGGAA 
CAGGCTTTGC AAGGTCTTTC AGAATTCAAG GCAGCCACGG TGTCTCACTG CGGCCCCGGG 
GCTGAGGAGG GTGAACAGGG GCCTGGGGGT GCCGGGGGCC GGCAGCTGGA GCCCAAAGCA 
GGCGGCGAGG CCTCGAGGGG CAGGGGCGCC CTCATCATTG TAGCTGTGGA GCAGAAAGGT 
CTTCAGGCCA GCAGGAGCAA TGCGGGGCCT GTTCCAGAAA CACTGCCCCG TGACTTTCCT 
AAGGAAAGAC CAGAATCTCC CTTGAGCACT GGCGAGACCC CCTGTGAGTC TCCCACTAGG 
GGGAAAACAC CAGCCGGTAA TGAGTGTGAG TTGCCAGCAG CCCCCATACA GGGAGCTGGC 
GATGGGGCTC TTCAGCGGGT GGCCCAGGCC GCAGAGCTTG GGAGAGTGCT GGTCCCCCAA 
GCTGCTTCGG AAGAGACGCC GAGCACAGAG GAGCCCCCGG GAGAGAGACT GCGTGGGGAG 
AGCCGGAGCT CCGGGTCAGG GGAGCGTGGC CCGGAGGAGG CCCCCGAAGG CGGTGCTGCA 
GCAGCCCGGG GCCAGCGCCC CCGCGTCCCC GCCTTGGAGC CGCCCCAGCC GCCACGCGGG 
CTCCGCAAGG GCGCGCAGGA GCCTGGGAAG CGCCCGACGT TTTCCAAGGT GACCTCCTTC 
AGGAAGGGCA GGCCCTTGGC CACTGAGAGC CCAGGAGGGG TCCCGGCCCC GACCACCGAG 
GGTCGCCGCT GGGGCTCTTC AGGCCCCGAG GGGCTCCCCA GGGAGAATCC GCCCGCTGCG 
GCCGGTCGGG ACGCACCGCC TCTGCACCAC GGGGACGCCA GCGCCTGGCC CGAGTTTGTC 
CCGCAGGCTG CAGGCGACAG GACTGCAGGG CCGGCAGGAG CGGGGCACAC GGGGACCTCA 
GGGGATCTTG GTAGCCGAGG GCCTTCCTCT GAGAGCTGCA ACGCAAAGAG ACTCAAAACA 
ACGGAGAAAA AACTCAGGGC AAGGTTGGCC TTGGCTCATA AGACCTTTTC AAACTTTATT 
GAGTCAATAG TTCTAGAGAA AGAGAACACC CATGAACGTT CCCCAAGTTC TCCCAAGGGC 
GAGAAGGAGA AGAGCAGGCT GCGCCAGGGT TCCTGGCGGG CGTTTCTGAA AAGCAAAGAT 
GCCGGAAGCC CCAAAAAGCC CACCCTAGTG AGTCTGCCTC TAGGACCCGA AGTTCTCTCC 
CCAGCAGAGA CCGACAGCCA CTGTGAGGAA CGGGCGGAGG ACAAAGAGGG CTATGTTTTT 
AGCGATCACT GGGCACCCCC ACTTGCCTCC ACACCTTTGT CCTCCAGTTT AGTTTCTCCA 
GAACACAGGA GGAAAAGTGA ACCGACCATC AAGTGCACAG CCACCCAGGA AGGCGACGCT 
AAACCAAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005263742.1
CDS	39..3170
Translation	MLSVVHFLRSFFKTPEPGAHLPGEGEIEDNQLPTSPAEQVEQGWNQQTDRDDSETLSQQSESGSDTKTDPFESASDTESLSGYLPRGVFHPLRGTPVDIEAPWEYPDVSATGPPQEQHLTSVPGLHAKEELDLSPSLEDDSCKNGWRAFATVAGEQEAGHLWDCATSLERESLLAGVPRHTGCCLQRATDSSGPEPVQGVAVQDLRGLSSVSLQKSRSESYLGIPVVWPFLLWCCELGRSWPHIHNRARALVLPSRGPLDNTAPLGTRTKKESTLGPAGDTELLWSQPHSDVPCQPPLRTSCLLRTNRHHSAPETTGDKNRASPRLNCGHMRALVRAHSIAGFSPECPEDPVGQNVVKSGTHVKEGAKNERDPRIQNIPSPAPTQLSGPIPAFQSGAPHLQGPCKPGGFRLQRASQDTPSAGLLGENQLRQDSRSCLVASCLTSELVKLSAEEVPEPAECKSEQSPESRTQEPQGTQLAPRAADERETQKHLWGISVQAGNQTSNYTSKYVLSEESKSPTRAKFPRQPSSEGTQVWSGDLMGCLEVSDSSDAPETTQKSSAIDTSKAAEEAMVLDPNYREQALQGLSEFKAATVSHCGPGAEEGEQGPGGAGGRQLEPKAGGEASRGRGALIIVAVEQKGLQASRSNAGPVPETLPRDFPKERPESPLSTGETPCESPTRGKTPAGNECELPAAPIQGAGDGALQRVAQAAELGRVLVPQAASEETPSTEEPPGERLRGESRSSGSGERGPEEAPEGGAAAARGQRPRVPALEPPQPPRGLRKGAQEPGKRPTFSKVTSFRKGRPLATESPGGVPAPTTEGRRWGSSGPEGLPRENPPAAAGRDAPPLHHGDASAWPEFVPQAAGDRTAGPAGAGHTGTSGDLGSRGPSSESCNAKRLKTTEKKLRARLALAHKTFSNFIESIVLEKENTHERSPSSPKGEKEKSRLRQGSWRAFLKSKDAGSPKKPTLVSLPLGPEVLSPAETDSHCEERAEDKEGYVFSDHWAPPLASTPLSSSLVSPEHRRKSEPTIKCTATQEGDAKPR

Target ORF information:

RefSeq Version	XM_005263685.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005263685.3

ORF Insert Sequence:

ATGCTCAGCG TCGTGCACTT CCTCCGGAGC TTCTTCAAGA CTCCAGAGCC AGGTGCACAC 
CTGCCAGGTG AAGGTGAGAT TGAAGATAAC CAGCTCCCCA CATCCCCTGC AGAACAAGTG 
GAGCAGGGAT GGAACCAGCA AACAGACCGC GATGATTCTG AAACGCTGTC CCAGCAGAGT 
GAAAGTGGAT CAGACACAAA AACTGACCCC TTTGAAAGTG CCTCTGACAC AGAGTCCTTG 
TCTGGGTACC TCCCGAGGGG AGTCTTCCAC CCTCTAAGAG GTACTCCCGT AGATATAGAA 
GCACCTTGGG AATACCCTGA TGTCTCAGCA ACTGGACCCC CTCAGGAGCA GCATTTGACC 
AGTGTTCCTG GGCTTCATGC AAAGGAAGAA CTCGATTTGT CCCCTAGCTT AGAGGATGAC 
TCTTGCAAAA ATGGGTGGCG AGCCTTTGCC ACAGTTGCTG GAGAACAGGA GGCAGGACAC 
CTCTGGGACT GCGCGACCAG CCTGGAGCGA GAGTCTTTGC TGGCAGGGGT TCCCCGACAC 
ACAGGGTGCT GCTTACAGAG GGCCACAGAC AGCAGTGGTC CTGAGCCAGT ACAGGGGGTG 
GCTGTTCAAG ACCTCAGAGG GCTCTCCAGT GTTTCTCTTC AGAAATCCAG GTCTGAGAGC 
TATCTGGGCA TCCCAGTGGT CTGGCCCTTC CTTCTCTGGT GCTGTGAACT GGGTCGGAGT 
TGGCCACATA TCCACAACAG GGCCAGGGCA CTGGTGCTAC CTAGCAGAGG CCCACTGGAC 
AACACAGCCC CTCTGGGGAC CAGGACAAAG AAGGAAAGTA CTCTAGGCCC TGCAGGGGAC 
ACAGAATTGC TCTGGTCCCA GCCCCACTCG GATGTCCCCT GCCAGCCTCC TTTGAGGACA 
TCTTGCCTCC TACGCACCAA CCGTCACCAT AGTGCCCCAG AAACTACTGG TGACAAGAAC 
AGGGCATCCC CCAGACTCAA CTGTGGGCAC ATGAGGGCAC TGGTCAGGGC CCATTCCATA 
GCAGGGTTTT CACCAGAGTG CCCCGAGGAT CCCGTGGGAC AGAATGTTGT GAAGTCTGGG 
ACCCATGTGA AGGAAGGGGC CAAAAATGAA CGAGATCCAA GAATACAAAA CATCCCTTCC 
CCTGCACCCA CCCAGCTGTC TGGCCCGATT CCTGCTTTTC AGAGTGGGGC TCCCCATCTG 
CAGGGTCCCT GCAAGCCTGG TGGGTTTCGC TTGCAAAGGG CCTCTCAGGA CACTCCTTCT 
GCAGGTCTCC TGGGGGAAAA CCAGTTAAGA CAGGATTCCA GGTCATGTCT GGTGGCTTCA 
TGCCTCACCT CAGAGTTAGT GAAGCTCAGT GCAGAGGAAG TGCCTGAGCC CGCTGAGTGC 
AAGTCAGAGC AAAGCCCAGA AAGCAGAACC CAGGAACCCC AAGGCACCCA ACTCGCACCA 
AGAGCTGCTG ATGAGAGAGA GACACAGAAG CACCTCTGGG GCATTTCTGT CCAGGCAGGA 
AACCAAACCA GTAATTACAC ATCAAAGTAT GTGCTCAGCG AGGAAAGCAA GTCACCTACC 
AGGGCCAAGT TCCCACGGCA GCCTAGCAGT GAGGGGACCC AGGTGTGGAG TGGAGACTTG 
ATGGGCTGCT TGGAAGTGAG TGACAGTTCA GATGCCCCTG AGACCACCCA GAAATCAAGC 
GCAATAGACA CTTCAAAGGC AGCCGAAGAA GCCATGGTTC TAGACCCCAA CTACAGGGAA 
CAGGCTTTGC AAGGTCTTTC AGAATTCAAG GCAGCCACGG TGTCTCACTG CGGCCCCGGG 
GCTGAGGAGG GTGAACAGGG GCCTGGGGGT GCCGGGGGCC GGCAGCTGGA GCCCAAAGCA 
GGCGGCGAGG CCTCGAGGGG CAGGGGCGCC CTCATCATTG TAGCTGTGGA GCAGAAAGGT 
CTTCAGGCCA GCAGGAGCAA TGCGGGGCCT GTTCCAGAAA CACTGCCCCG TGACTTTCCT 
AAGGAAAGAC CAGAATCTCC CTTGAGCACT GGCGAGACCC CCTGTGAGTC TCCCACTAGG 
GGGAAAACAC CAGCCGGTAA TGAGTGTGAG TTGCCAGCAG CCCCCATACA GGGAGCTGGC 
GATGGGGCTC TTCAGCGGGT GGCCCAGGCC GCAGAGCTTG GGAGAGTGCT GGTCCCCCAA 
GCTGCTTCGG AAGAGACGCC GAGCACAGAG GAGCCCCCGG GAGAGAGACT GCGTGGGGAG 
AGCCGGAGCT CCGGGTCAGG GGAGCGTGGC CCGGAGGAGG CCCCCGAAGG CGGTGCTGCA 
GCAGCCCGGG GCCAGCGCCC CCGCGTCCCC GCCTTGGAGC CGCCCCAGCC GCCACGCGGG 
CTCCGCAAGG GCGCGCAGGA GCCTGGGAAG CGCCCGACGT TTTCCAAGGT GACCTCCTTC 
AGGAAGGGCA GGCCCTTGGC CACTGAGAGC CCAGGAGGGG TCCCGGCCCC GACCACCGAG 
GGTCGCCGCT GGGGCTCTTC AGGCCCCGAG GGGCTCCCCA GGGAGAATCC GCCCGCTGCG 
GCCGGTCGGG ACGCACCGCC TCTGCACCAC GGGGACGCCA GCGCCTGGCC CGAGTTTGTC 
CCGCAGGCTG CAGGCGACAG GACTGCAGGG CCGGCAGGAG CGGGGCACAC GGGGACCTCA 
GGGGATCTTG GTAGCCGAGG GCCTTCCTCT GAGAGCTGCA ACGCAAAGAG ACTCAAAACA 
ACGGAGAAAA AACTCAGGGC AAGGTTGGCC TTGGCTCATA AGACCTTTTC AAACTTTATT 
GAGTCAATAG TTCTAGAGAA AGAGAACACC CATGAACGTT CCCCAAGTTC TCCCAAGGGC 
GAGAAGGAGA AGAGCAGGCT GCGCCAGGGT TCCTGGCGGG CGTTTCTGAA AAGCAAAGAT 
GCCGGAAGCC CCAAAAAGCC CACCCTAGTG AGTCTGCCTC TAGGACCCGA AGTTCTCTCC 
CCAGCAGAGA CCGACAGCCA CTGTGAGGAA CGGGCGGAGG ACAAAGAGGG CTATGTTTTT 
AGCGATCACT GGGCACCCCC ACTTGCCTCC ACACCTTTGT CCTCCAGTTT AGTTTCTCCA 
GAACACAGGA GGAAAAGTGA ACCGACCATC AAGTGCACAG CCACCCAGGA AGGCGACGCT 
AAACCAAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF4 ( XM_011511274.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509576.1 Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X2, mRNA.

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CloneID	OHu67537
Clone ID Related Accession (Same CDS sequence)	XM_011511274.2 , XM_011511274.3
Accession Version	XM_011511274.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011511274.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAGCG TCGTGCACTT CCTCCGGAGC TTCTTCAAGA CTCCAGAGCC AGGTGCACAC 
CTGCCAGGTG AAGGTGAGAT TGAAGATAAC CAGCTCCCCA CATCCCCTGC AGAACAAGTG 
GAGCAGGGAT GGAACCAGCA AACAGACCGC GATGATTCTG AAACGCTGTC CCAGCAGAGT 
GAAAGTGGAT CAGACACAAA AACTGACCCC TTTGAAAGTG CCTCTGACAC AGAGTCCTTG 
TCTGGGTACC TCCCGAGGGG AGTCTTCCAC CCTCTAAGAG GTACTCCCGT AGATATAGAA 
GCACCTTGGG AATACCCTGA TGTCTCAGCA ACTGGACCCC CTCAGGAGCA GCATTTGACC 
AGTGTTCCTG GGCTTCATGC AAAGGAAGAA CTCGATTTGT CCCCTAGCTT AGAGGATGAC 
TCTTGCAAAA ATGGGTGGCG AGCCTTTGCC ACAGTTGCTG GAGAACAGGA GGCAGGACAC 
CTCTGGGACT GCGCGACCAG CCTGGAGCGA GAGTCTTTGC TGGCAGGGGT TCCCCGACAC 
ACAGGGTGCT GCTTACAGAG GGCCACAGAC AGCAGTGGTC CTGAGCCAGT ACAGGGGGTG 
GCTGTTCAAG ACCTCAGAGG GCTCTCCAGT GTTTCTCTTC AGAAATCCAG GTCTGAGAGC 
TATCTGGGCA TCCCAGTGGT CTGGCCCTTC CTTCTCTGGT GCTGTGAACT GGGTCGGAGT 
TGGCCACATA TCCACAACAG GGCCAGGGCA CTGGTGCTAC CTAGCAGAGG CCCACTGGAC 
AACACAGCCC CTCTGGGGAC CAGGACAAAG AAGGAAAGTA CTCTAGGCCC TGCAGGGGAC 
ACAGAATTGC TCTGGTCCCA GCCCCACTCG GATGTCCCCT GCCAGCCTCC TTTGAGGACA 
TCTTGCCTCC TACGCACCAA CCGTCACCAT AGTGCCCCAG AAACTACTGG TGACAAGAAC 
AGGGCATCCC CCAGACTCAA CTGTGGGCAC ATGAGGGCAC TGGTCAGGGC CCATTCCATA 
GCAGGGTTTT CACCAGAGTG CCCCGAGGAT CCCGTGGGAC AGAATGTTGT GAAGTCTGGG 
ACCCATGTGA AGGAAGGGGC CAAAAATGAA CGAGATCCAA GAATACAAAA CATCCCTTCC 
CCTGCACCCA CCCAGCTGTC TGGCCCGATT CCTGCTTTTC AGAGTGGGGC TCCCCATCTG 
CAGGGTCCCT GCAAGCCTGG TGGGTTTCGC TTGCAAAGGG CCTCTCAGGA CACTCCTTCT 
GCAGGTCTCC TGGGGGAAAA CCAGTTAAGA CAGGATTCCA GGTCATGTCT GGTGGCTTCA 
TGCCTCACCT CAGAGTTAGT GAAGCTCAGT GCAGAGGAAG TGCCTGAGCC CGCTGAGTGC 
AAGTCAGAGC AAAGCCCAGA AAGCAGAACC CAGGAACCCC AAGGCACCCA ACTCGCACCA 
AGAGCTGCTG ATGAGAGAGA GACACAGAAG CACCTCTGGG GCATTTCTGT CCAGGCAGGA 
AACCAAACCA GTAATTACAC ATCAAAGTAT GTGCTCAGCG AGGAAAGCAA GTCACCTACC 
AGGGCCAAGT TCCCACGGCA GCCTAGCAGT GAGGGGACCC AGGTGTGGAG TGGAGACTTG 
ATGGGCTGCT TGGAAGTGAG TGACAGTTCA GATGCCCCTG AGACCACCCA GAAATCAAGC 
GCAATAGACA CTTCAAAGGC AGCCGAAGAA GCCATGGTTC TAGACCCCAA CTACAGGGAA 
CAGGCTTTGC AAGGTCTTTC AGAATTCAAG GCAGCCACGG TGTCTCACTG CGGCCCCGGG 
GCTGAGGAGG GTGAACAGGG GCCTGGGGGT GCCGGGGGCC GGCAGCTGGA GCCCAAAGCA 
GGCGGCGAGG CCTCGAGGGG CAGGGGCGCC CTCATCATTG TAGCTGTGGA GCAGAAAGGT 
CTTCAGGCCA GCAGGAGCAA TGCGGGGCCT GTTCCAGAAA CACTGCCCCG TGACTTTCCT 
AAGGAAAGAC CAGAATCTCC CTTGAGCACT GGCGAGACCC CCTGTGAGTC TCCCACTAGG 
GGGAAAACAC CAGCCGGTAA TGAGTGTGAG TTGCCAGCAG CCCCCATACA GGGAGCTGGC 
GATGGGGCTC TTCAGCGGGT GGCCCAGGCC GCAGAGCTTG GGAGAGTGCT GGTCCCCCAA 
GCTGCTTCGG AAGAGACGCC GAGCACAGAG GAGCCCCCGG GAGAGAGACT GCGTGGGGAG 
AGCCGGAGCT CCGGGTCAGG GGAGCGTGGC CCGGAGGAGG CCCCCGAAGG CGGTGCTGCA 
GCAGCCCGGG GCCAGCGCCC CCGCGTCCCC GCCTTGGAGC CGCCCCAGCC GCCACGCGGG 
CTCCGCAAGG GCGCGCAGGA GCCTGGGAAG CGCCCGACGT TTTCCAAGGT GACCTCCTTC 
AGGAAGGGCA GGCCCTTGGC CACTGAGAGC CCAGGAGGGG TCCCGGCCCC GACCACCGAG 
GGTCGCCGCT GGGGCTCTTC AGGCCCCGAG GGGCTCCCCA GGGAGAATCC GCCCGCTGCG 
GCCGGTCGGG ACGCACCGCC TCTGCACCAC GGGGACGCCA GCGCCTGGCC CGAGTTTGTC 
CCGCAGGCTG CAGGCGACAG GACTGCAGGG CCGGCAGGAG CGGGGCACAC GGGGACCTCA 
GGGGATCTTG GTAGCCGAGG GCCTTCCTCT GAGAGCTGCA ACGCAAAGAG ACTCAAAACA 
ACGGAGAAAA AACTCAGGGC AAGGTTGGCC TTGGCTCATA AGACCTTTTC AAACTTTATT 
GAGTCAATAG TTCTAGAGAA AGAGAACACC CATGAACGTT CCCCAAGTTC TCCCAAGGGC 
GAGAAGGAGA AGAGCAGGCT GCGCCAGGGT TCCTGGCGGG CGTTTCTGAA AAGCAAAGAT 
GCCGGAAGCC CCAAAAAGCC CACCCTAGTG AGTCTGCCTC TAGGACCCGA AGTTCTCTCC 
CCAGCAGAGA CCGACAGCCA CTGTGAGGAA CGGGCGGAGG ACAAAGAGGG CTATGTTTTT 
AGCGATCACT GGGCACCCCC ACTTGCCTCC ACACCTTTGT CCTCCAGTTT AGTTTCTCCA 
GAACACAGGA GGAAAAGTGA ACCGACCATC AAGTGCACAG CCACCCAGGA AGGCGGTAGG 
TACCTACCTT CAGGTATCTT TCCGGAAAAG TCCTGGCTGG CGTCCCCCGG CAGCCCTCGG 
GCCCAGCAGG CTGGAATCGC ACACACCCTG CCTTCCAGCT CTGCCTGCTG CCTGGCATAT 
GAAAACCCCG GGACGCCCTG CAGACCCACG AGCCCCAAGC CCCTGAGTCC CAGGCCTAGT 
GCTCAGCGGA TGGGTCTCCA CTACCCCGGG AGGGGTAGCG CCATCTCCAT GGTTTCTCTT 
GGAAGCTACA GCTACGTGGA CAGCAGTTCA GGGGACCCTG AAAGACCCAA GATTCCCAAG 
GGCCAGACCA GTTTCCTGCT TTCTCTGCAG ACGCTAAACC AAGATGAGCA GAAGGAAGAG 
AGCAGGGAAG GAGGCCAGGG TCCGCGCGGC TTGGGCACAG TGCCCTGGCT CAGGGACCTT 
CCTGGGAGTG AGAACCACAT GCCCTGGGAA GAACCAGCAG GTGAGAAGCC CAGTTGCTCT 
CACAGTCAGA AGGCGTTCCA CATGGAGCCT GCCCAGAAGC CCTGCTTCAC CACTGACATG 
GTGACATGGG CCCTCCTCTG CATCTCTGCA GAGACTGTGC GTGGGGAGGC TCCTTCACAG 
CCTAGGGGCA TCCCTCACCG CTCGCCCGTC AGTGTGGATG ACCTGTGGCT GGAGAAGACA 
CAGAGAAAGA AGTTGCAGAA GCAGGCCCAC GTCGAAAGGA GGCTGCACAT AGGGGCAGTG 
CACAAAGATG GAGTCAAGTG CTGGAGAAAG ACGATCATTA CCTCTCCAGA GTCTTTGAAT 
CTCCCTAGAA GAAGCCATCC ACTCTCCCAG AGTGCTCCAA CGGGACTGAA CCACATGGGC 
TGGCCAGAGC ACACACCAGG CACTGCCATG CCTGATGGAG CTCTGGACAC AGCTGTCTGC 
GCTGACGAAG TGGGGAGCGA GGAGGACCTG TATGATGACC TGCACAGCTC CAGCCACCAC 
TACAGCCACC CTGGAGGGGG TGGGGAGCAG CTGGCTATCA ATGAGCTCAT CAGCGATGGC 
AGTGTGGTCT GCGCTGAAGC ACTCTGGGAC CATGTCACCA TGGACGACCA GGAGCTGGGC 
TTCAAAGCTG GGGACGTCAT CGAAGTGATG GATGCCACCA ACAGAGAGTG GTGGTGGGGC 
CGGGTCGCCG ATGGCGAGGG CTGGTTTCCA GCCAGCTTCG TTCGGCTGAG GGTGAATCAG 
GACGAGCCCG CGGATGACGA CGCCCCTCTG GCCGGGAACA GCGGAGCGGA GGACGGCGGG 
GCGGAGGCGC AGAGCAGCAA GGACCAGATG CGGACCAACG TCATCAACGA GATCCTCAGC 
ACTGAGCGGG ACTACATCAA GCACCTGCGC GACATCTGCG AGGGCTACGT CCGGCAGTGC 
CGCAAGCGCG CAGACATGTT CAGCGAGGAG CAGCTGCGTA CCATCTTCGG GAACATCGAG 
GACATCTACC GCTGCCAGAA GGCCTTCGTG AAGGCCCTGG AGCAGAGGTT CAACCGCGAG 
CGCCCACACC TGAGCGAGCT GGGTGCCTGC TTCCTGGAGC ATCAAGCCGA CTTCCAGATC 
TACTCGGAGT ACTGCAATAA CCACCCCAAC GCCTGCGTGG AGCTCTCCCG GCTCACCAAG 
CTCAGCAAGT ACGTGTACTT CTTCGAGGCC TGCCGGCTGC TGCAGAAGAT GATTGACATC 
TCCCTGGATG GCTTCCTGCT GACTCCGGTG CAGAAGATCT GCAAGTACCC TCTGCAGCTG 
GCCGAGCTGC TCAAATACAC GCACCCCCAG CACAGGGACT TCAAGGATGT TGAAGCCGCC 
TTGCATGCCA TGAAGAACGT GGCCCAGCTC ATCAACGAGC GGAAGCGGAG ACTTGAGAAC 
ATCGACAAGA TTGCTCAGTG GCAGAGCTCC ATAGAGGACT GGGAGGGAGA AGATCTCTTG 
GTCAGGAGCT CAGAACTCAT CTACTCGGGG GAGCTGACTC GAGTTACACA GCCTCAAGCC 
AAAAGCCAGC AGCGAATGTT CTTTCTCTTT GACCACCAGC TCATCTACTG TAAGAAGGAC 
CTGCTCCGCC GCGACGTGTT GTACTACAAG GGCCGGCTGG ACATGGACGG CCTGGAGGTG 
GTGGACCTGG AGGACGGGAA GGACAGAGAC CTCCATGTGA GCATCAAGAA CGCCTTCCGG 
CTGCACCGTG GCGCCACAGG GGACAGCCAC CTGCTGTGCA CCAGGAAGCC CGAGCAGAAG 
CAGCGCTGGC TCAAGGCCTT TGCCAGGGAG AGGGAGCAGG TGCAGCTGGA CCAGGAGACA 
GGCTTCTCCA TCACTGAACT GCAGAGGAAG CAGGCCATGC TGAATGCCAG CAAGCAGCAG 
GTCACAGGGA AGCCCAAAGG CCACCCCCCT CTTCAGGCTG CTGCAGGAGG CCAGGCCCAG 
GCCAGAGACC CCAGGCAGCC TGAGCTCTTG GATGTGGTCA GCTCTCCAGG AGCAAGTCCT 
GTGTGGCAAA ACTGCCTCCT ACGGCGGCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509576.1
CDS	39..5651
Translation	MLSVVHFLRSFFKTPEPGAHLPGEGEIEDNQLPTSPAEQVEQGWNQQTDRDDSETLSQQSESGSDTKTDPFESASDTESLSGYLPRGVFHPLRGTPVDIEAPWEYPDVSATGPPQEQHLTSVPGLHAKEELDLSPSLEDDSCKNGWRAFATVAGEQEAGHLWDCATSLERESLLAGVPRHTGCCLQRATDSSGPEPVQGVAVQDLRGLSSVSLQKSRSESYLGIPVVWPFLLWCCELGRSWPHIHNRARALVLPSRGPLDNTAPLGTRTKKESTLGPAGDTELLWSQPHSDVPCQPPLRTSCLLRTNRHHSAPETTGDKNRASPRLNCGHMRALVRAHSIAGFSPECPEDPVGQNVVKSGTHVKEGAKNERDPRIQNIPSPAPTQLSGPIPAFQSGAPHLQGPCKPGGFRLQRASQDTPSAGLLGENQLRQDSRSCLVASCLTSELVKLSAEEVPEPAECKSEQSPESRTQEPQGTQLAPRAADERETQKHLWGISVQAGNQTSNYTSKYVLSEESKSPTRAKFPRQPSSEGTQVWSGDLMGCLEVSDSSDAPETTQKSSAIDTSKAAEEAMVLDPNYREQALQGLSEFKAATVSHCGPGAEEGEQGPGGAGGRQLEPKAGGEASRGRGALIIVAVEQKGLQASRSNAGPVPETLPRDFPKERPESPLSTGETPCESPTRGKTPAGNECELPAAPIQGAGDGALQRVAQAAELGRVLVPQAASEETPSTEEPPGERLRGESRSSGSGERGPEEAPEGGAAAARGQRPRVPALEPPQPPRGLRKGAQEPGKRPTFSKVTSFRKGRPLATESPGGVPAPTTEGRRWGSSGPEGLPRENPPAAAGRDAPPLHHGDASAWPEFVPQAAGDRTAGPAGAGHTGTSGDLGSRGPSSESCNAKRLKTTEKKLRARLALAHKTFSNFIESIVLEKENTHERSPSSPKGEKEKSRLRQGSWRAFLKSKDAGSPKKPTLVSLPLGPEVLSPAETDSHCEERAEDKEGYVFSDHWAPPLASTPLSSSLVSPEHRRKSEPTIKCTATQEGGRYLPSGIFPEKSWLASPGSPRAQQAGIAHTLPSSSACCLAYENPGTPCRPTSPKPLSPRPSAQRMGLHYPGRGSAISMVSLGSYSYVDSSSGDPERPKIPKGQTSFLLSLQTLNQDEQKEESREGGQGPRGLGTVPWLRDLPGSENHMPWEEPAGEKPSCSHSQKAFHMEPAQKPCFTTDMVTWALLCISAETVRGEAPSQPRGIPHRSPVSVDDLWLEKTQRKKLQKQAHVERRLHIGAVHKDGVKCWRKTIITSPESLNLPRRSHPLSQSAPTGLNHMGWPEHTPGTAMPDGALDTAVCADEVGSEEDLYDDLHSSSHHYSHPGGGGEQLAINELISDGSVVCAEALWDHVTMDDQELGFKAGDVIEVMDATNREWWWGRVADGEGWFPASFVRLRVNQDEPADDDAPLAGNSGAEDGGAEAQSSKDQMRTNVINEILSTERDYIKHLRDICEGYVRQCRKRADMFSEEQLRTIFGNIEDIYRCQKAFVKALEQRFNRERPHLSELGACFLEHQADFQIYSEYCNNHPNACVELSRLTKLSKYVYFFEACRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTHPQHRDFKDVEAALHAMKNVAQLINERKRRLENIDKIAQWQSSIEDWEGEDLLVRSSELIYSGELTRVTQPQAKSQQRMFFLFDHQLIYCKKDLLRRDVLYYKGRLDMDGLEVVDLEDGKDRDLHVSIKNAFRLHRGATGDSHLLCTRKPEQKQRWLKAFAREREQVQLDQETGFSITELQRKQAMLNASKQQVTGKPKGHPPLQAAAGGQAQARDPRQPELLDVVSSPGASPVWQNCLLRRL

Target ORF information:

RefSeq Version	XM_011511274.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511274.2

ORF Insert Sequence:

ATGCTCAGCG TCGTGCACTT CCTCCGGAGC TTCTTCAAGA CTCCAGAGCC AGGTGCACAC 
CTGCCAGGTG AAGGTGAGAT TGAAGATAAC CAGCTCCCCA CATCCCCTGC AGAACAAGTG 
GAGCAGGGAT GGAACCAGCA AACAGACCGC GATGATTCTG AAACGCTGTC CCAGCAGAGT 
GAAAGTGGAT CAGACACAAA AACTGACCCC TTTGAAAGTG CCTCTGACAC AGAGTCCTTG 
TCTGGGTACC TCCCGAGGGG AGTCTTCCAC CCTCTAAGAG GTACTCCCGT AGATATAGAA 
GCACCTTGGG AATACCCTGA TGTCTCAGCA ACTGGACCCC CTCAGGAGCA GCATTTGACC 
AGTGTTCCTG GGCTTCATGC AAAGGAAGAA CTCGATTTGT CCCCTAGCTT AGAGGATGAC 
TCTTGCAAAA ATGGGTGGCG AGCCTTTGCC ACAGTTGCTG GAGAACAGGA GGCAGGACAC 
CTCTGGGACT GCGCGACCAG CCTGGAGCGA GAGTCTTTGC TGGCAGGGGT TCCCCGACAC 
ACAGGGTGCT GCTTACAGAG GGCCACAGAC AGCAGTGGTC CTGAGCCAGT ACAGGGGGTG 
GCTGTTCAAG ACCTCAGAGG GCTCTCCAGT GTTTCTCTTC AGAAATCCAG GTCTGAGAGC 
TATCTGGGCA TCCCAGTGGT CTGGCCCTTC CTTCTCTGGT GCTGTGAACT GGGTCGGAGT 
TGGCCACATA TCCACAACAG GGCCAGGGCA CTGGTGCTAC CTAGCAGAGG CCCACTGGAC 
AACACAGCCC CTCTGGGGAC CAGGACAAAG AAGGAAAGTA CTCTAGGCCC TGCAGGGGAC 
ACAGAATTGC TCTGGTCCCA GCCCCACTCG GATGTCCCCT GCCAGCCTCC TTTGAGGACA 
TCTTGCCTCC TACGCACCAA CCGTCACCAT AGTGCCCCAG AAACTACTGG TGACAAGAAC 
AGGGCATCCC CCAGACTCAA CTGTGGGCAC ATGAGGGCAC TGGTCAGGGC CCATTCCATA 
GCAGGGTTTT CACCAGAGTG CCCCGAGGAT CCCGTGGGAC AGAATGTTGT GAAGTCTGGG 
ACCCATGTGA AGGAAGGGGC CAAAAATGAA CGAGATCCAA GAATACAAAA CATCCCTTCC 
CCTGCACCCA CCCAGCTGTC TGGCCCGATT CCTGCTTTTC AGAGTGGGGC TCCCCATCTG 
CAGGGTCCCT GCAAGCCTGG TGGGTTTCGC TTGCAAAGGG CCTCTCAGGA CACTCCTTCT 
GCAGGTCTCC TGGGGGAAAA CCAGTTAAGA CAGGATTCCA GGTCATGTCT GGTGGCTTCA 
TGCCTCACCT CAGAGTTAGT GAAGCTCAGT GCAGAGGAAG TGCCTGAGCC CGCTGAGTGC 
AAGTCAGAGC AAAGCCCAGA AAGCAGAACC CAGGAACCCC AAGGCACCCA ACTCGCACCA 
AGAGCTGCTG ATGAGAGAGA GACACAGAAG CACCTCTGGG GCATTTCTGT CCAGGCAGGA 
AACCAAACCA GTAATTACAC ATCAAAGTAT GTGCTCAGCG AGGAAAGCAA GTCACCTACC 
AGGGCCAAGT TCCCACGGCA GCCTAGCAGT GAGGGGACCC AGGTGTGGAG TGGAGACTTG 
ATGGGCTGCT TGGAAGTGAG TGACAGTTCA GATGCCCCTG AGACCACCCA GAAATCAAGC 
GCAATAGACA CTTCAAAGGC AGCCGAAGAA GCCATGGTTC TAGACCCCAA CTACAGGGAA 
CAGGCTTTGC AAGGTCTTTC AGAATTCAAG GCAGCCACGG TGTCTCACTG CGGCCCCGGG 
GCTGAGGAGG GTGAACAGGG GCCTGGGGGT GCCGGGGGCC GGCAGCTGGA GCCCAAAGCA 
GGCGGCGAGG CCTCGAGGGG CAGGGGCGCC CTCATCATTG TAGCTGTGGA GCAGAAAGGT 
CTTCAGGCCA GCAGGAGCAA TGCGGGGCCT GTTCCAGAAA CACTGCCCCG TGACTTTCCT 
AAGGAAAGAC CAGAATCTCC CTTGAGCACT GGCGAGACCC CCTGTGAGTC TCCCACTAGG 
GGGAAAACAC CAGCCGGTAA TGAGTGTGAG TTGCCAGCAG CCCCCATACA GGGAGCTGGC 
GATGGGGCTC TTCAGCGGGT GGCCCAGGCC GCAGAGCTTG GGAGAGTGCT GGTCCCCCAA 
GCTGCTTCGG AAGAGACGCC GAGCACAGAG GAGCCCCCGG GAGAGAGACT GCGTGGGGAG 
AGCCGGAGCT CCGGGTCAGG GGAGCGTGGC CCGGAGGAGG CCCCCGAAGG CGGTGCTGCA 
GCAGCCCGGG GCCAGCGCCC CCGCGTCCCC GCCTTGGAGC CGCCCCAGCC GCCACGCGGG 
CTCCGCAAGG GCGCGCAGGA GCCTGGGAAG CGCCCGACGT TTTCCAAGGT GACCTCCTTC 
AGGAAGGGCA GGCCCTTGGC CACTGAGAGC CCAGGAGGGG TCCCGGCCCC GACCACCGAG 
GGTCGCCGCT GGGGCTCTTC AGGCCCCGAG GGGCTCCCCA GGGAGAATCC GCCCGCTGCG 
GCCGGTCGGG ACGCACCGCC TCTGCACCAC GGGGACGCCA GCGCCTGGCC CGAGTTTGTC 
CCGCAGGCTG CAGGCGACAG GACTGCAGGG CCGGCAGGAG CGGGGCACAC GGGGACCTCA 
GGGGATCTTG GTAGCCGAGG GCCTTCCTCT GAGAGCTGCA ACGCAAAGAG ACTCAAAACA 
ACGGAGAAAA AACTCAGGGC AAGGTTGGCC TTGGCTCATA AGACCTTTTC AAACTTTATT 
GAGTCAATAG TTCTAGAGAA AGAGAACACC CATGAACGTT CCCCAAGTTC TCCCAAGGGC 
GAGAAGGAGA AGAGCAGGCT GCGCCAGGGT TCCTGGCGGG CGTTTCTGAA AAGCAAAGAT 
GCCGGAAGCC CCAAAAAGCC CACCCTAGTG AGTCTGCCTC TAGGACCCGA AGTTCTCTCC 
CCAGCAGAGA CCGACAGCCA CTGTGAGGAA CGGGCGGAGG ACAAAGAGGG CTATGTTTTT 
AGCGATCACT GGGCACCCCC ACTTGCCTCC ACACCTTTGT CCTCCAGTTT AGTTTCTCCA 
GAACACAGGA GGAAAAGTGA ACCGACCATC AAGTGCACAG CCACCCAGGA AGGCGGTAGG 
TACCTACCTT CAGGTATCTT TCCGGAAAAG TCCTGGCTGG CGTCCCCCGG CAGCCCTCGG 
GCCCAGCAGG CTGGAATCGC ACACACCCTG CCTTCCAGCT CTGCCTGCTG CCTGGCATAT 
GAAAACCCCG GGACGCCCTG CAGACCCACG AGCCCCAAGC CCCTGAGTCC CAGGCCTAGT 
GCTCAGCGGA TGGGTCTCCA CTACCCCGGG AGGGGTAGCG CCATCTCCAT GGTTTCTCTT 
GGAAGCTACA GCTACGTGGA CAGCAGTTCA GGGGACCCTG AAAGACCCAA GATTCCCAAG 
GGCCAGACCA GTTTCCTGCT TTCTCTGCAG ACGCTAAACC AAGATGAGCA GAAGGAAGAG 
AGCAGGGAAG GAGGCCAGGG TCCGCGCGGC TTGGGCACAG TGCCCTGGCT CAGGGACCTT 
CCTGGGAGTG AGAACCACAT GCCCTGGGAA GAACCAGCAG GTGAGAAGCC CAGTTGCTCT 
CACAGTCAGA AGGCGTTCCA CATGGAGCCT GCCCAGAAGC CCTGCTTCAC CACTGACATG 
GTGACATGGG CCCTCCTCTG CATCTCTGCA GAGACTGTGC GTGGGGAGGC TCCTTCACAG 
CCTAGGGGCA TCCCTCACCG CTCGCCCGTC AGTGTGGATG ACCTGTGGCT GGAGAAGACA 
CAGAGAAAGA AGTTGCAGAA GCAGGCCCAC GTCGAAAGGA GGCTGCACAT AGGGGCAGTG 
CACAAAGATG GAGTCAAGTG CTGGAGAAAG ACGATCATTA CCTCTCCAGA GTCTTTGAAT 
CTCCCTAGAA GAAGCCATCC ACTCTCCCAG AGTGCTCCAA CGGGACTGAA CCACATGGGC 
TGGCCAGAGC ACACACCAGG CACTGCCATG CCTGATGGAG CTCTGGACAC AGCTGTCTGC 
GCTGACGAAG TGGGGAGCGA GGAGGACCTG TATGATGACC TGCACAGCTC CAGCCACCAC 
TACAGCCACC CTGGAGGGGG TGGGGAGCAG CTGGCTATCA ATGAGCTCAT CAGCGATGGC 
AGTGTGGTCT GCGCTGAAGC ACTCTGGGAC CATGTCACCA TGGACGACCA GGAGCTGGGC 
TTCAAAGCTG GGGACGTCAT CGAAGTGATG GATGCCACCA ACAGAGAGTG GTGGTGGGGC 
CGGGTCGCCG ATGGCGAGGG CTGGTTTCCA GCCAGCTTCG TTCGGCTGAG GGTGAATCAG 
GACGAGCCCG CGGATGACGA CGCCCCTCTG GCCGGGAACA GCGGAGCGGA GGACGGCGGG 
GCGGAGGCGC AGAGCAGCAA GGACCAGATG CGGACCAACG TCATCAACGA GATCCTCAGC 
ACTGAGCGGG ACTACATCAA GCACCTGCGC GACATCTGCG AGGGCTACGT CCGGCAGTGC 
CGCAAGCGCG CAGACATGTT CAGCGAGGAG CAGCTGCGTA CCATCTTCGG GAACATCGAG 
GACATCTACC GCTGCCAGAA GGCCTTCGTG AAGGCCCTGG AGCAGAGGTT CAACCGCGAG 
CGCCCACACC TGAGCGAGCT GGGTGCCTGC TTCCTGGAGC ATCAAGCCGA CTTCCAGATC 
TACTCGGAGT ACTGCAATAA CCACCCCAAC GCCTGCGTGG AGCTCTCCCG GCTCACCAAG 
CTCAGCAAGT ACGTGTACTT CTTCGAGGCC TGCCGGCTGC TGCAGAAGAT GATTGACATC 
TCCCTGGATG GCTTCCTGCT GACTCCGGTG CAGAAGATCT GCAAGTACCC TCTGCAGCTG 
GCCGAGCTGC TCAAATACAC GCACCCCCAG CACAGGGACT TCAAGGATGT TGAAGCCGCC 
TTGCATGCCA TGAAGAACGT GGCCCAGCTC ATCAACGAGC GGAAGCGGAG ACTTGAGAAC 
ATCGACAAGA TTGCTCAGTG GCAGAGCTCC ATAGAGGACT GGGAGGGAGA AGATCTCTTG 
GTCAGGAGCT CAGAACTCAT CTACTCGGGG GAGCTGACTC GAGTTACACA GCCTCAAGCC 
AAAAGCCAGC AGCGAATGTT CTTTCTCTTT GACCACCAGC TCATCTACTG TAAGAAGGAC 
CTGCTCCGCC GCGACGTGTT GTACTACAAG GGCCGGCTGG ACATGGACGG CCTGGAGGTG 
GTGGACCTGG AGGACGGGAA GGACAGAGAC CTCCATGTGA GCATCAAGAA CGCCTTCCGG 
CTGCACCGTG GCGCCACAGG GGACAGCCAC CTGCTGTGCA CCAGGAAGCC CGAGCAGAAG 
CAGCGCTGGC TCAAGGCCTT TGCCAGGGAG AGGGAGCAGG TGCAGCTGGA CCAGGAGACA 
GGCTTCTCCA TCACTGAACT GCAGAGGAAG CAGGCCATGC TGAATGCCAG CAAGCAGCAG 
GTCACAGGGA AGCCCAAAGG CCACCCCCCT CTTCAGGCTG CTGCAGGAGG CCAGGCCCAG 
GCCAGAGACC CCAGGCAGCC TGAGCTCTTG GATGTGGTCA GCTCTCCAGG AGCAAGTCCT 
GTGTGGCAAA ACTGCCTCCT ACGGCGGCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF4 ( XM_011511274.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509576.1 Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X2, mRNA.

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CloneID	OHu67537
Clone ID Related Accession (Same CDS sequence)	XM_011511274.2 , XM_011511274.3
Accession Version	XM_011511274.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011511274.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAGCG TCGTGCACTT CCTCCGGAGC TTCTTCAAGA CTCCAGAGCC AGGTGCACAC 
CTGCCAGGTG AAGGTGAGAT TGAAGATAAC CAGCTCCCCA CATCCCCTGC AGAACAAGTG 
GAGCAGGGAT GGAACCAGCA AACAGACCGC GATGATTCTG AAACGCTGTC CCAGCAGAGT 
GAAAGTGGAT CAGACACAAA AACTGACCCC TTTGAAAGTG CCTCTGACAC AGAGTCCTTG 
TCTGGGTACC TCCCGAGGGG AGTCTTCCAC CCTCTAAGAG GTACTCCCGT AGATATAGAA 
GCACCTTGGG AATACCCTGA TGTCTCAGCA ACTGGACCCC CTCAGGAGCA GCATTTGACC 
AGTGTTCCTG GGCTTCATGC AAAGGAAGAA CTCGATTTGT CCCCTAGCTT AGAGGATGAC 
TCTTGCAAAA ATGGGTGGCG AGCCTTTGCC ACAGTTGCTG GAGAACAGGA GGCAGGACAC 
CTCTGGGACT GCGCGACCAG CCTGGAGCGA GAGTCTTTGC TGGCAGGGGT TCCCCGACAC 
ACAGGGTGCT GCTTACAGAG GGCCACAGAC AGCAGTGGTC CTGAGCCAGT ACAGGGGGTG 
GCTGTTCAAG ACCTCAGAGG GCTCTCCAGT GTTTCTCTTC AGAAATCCAG GTCTGAGAGC 
TATCTGGGCA TCCCAGTGGT CTGGCCCTTC CTTCTCTGGT GCTGTGAACT GGGTCGGAGT 
TGGCCACATA TCCACAACAG GGCCAGGGCA CTGGTGCTAC CTAGCAGAGG CCCACTGGAC 
AACACAGCCC CTCTGGGGAC CAGGACAAAG AAGGAAAGTA CTCTAGGCCC TGCAGGGGAC 
ACAGAATTGC TCTGGTCCCA GCCCCACTCG GATGTCCCCT GCCAGCCTCC TTTGAGGACA 
TCTTGCCTCC TACGCACCAA CCGTCACCAT AGTGCCCCAG AAACTACTGG TGACAAGAAC 
AGGGCATCCC CCAGACTCAA CTGTGGGCAC ATGAGGGCAC TGGTCAGGGC CCATTCCATA 
GCAGGGTTTT CACCAGAGTG CCCCGAGGAT CCCGTGGGAC AGAATGTTGT GAAGTCTGGG 
ACCCATGTGA AGGAAGGGGC CAAAAATGAA CGAGATCCAA GAATACAAAA CATCCCTTCC 
CCTGCACCCA CCCAGCTGTC TGGCCCGATT CCTGCTTTTC AGAGTGGGGC TCCCCATCTG 
CAGGGTCCCT GCAAGCCTGG TGGGTTTCGC TTGCAAAGGG CCTCTCAGGA CACTCCTTCT 
GCAGGTCTCC TGGGGGAAAA CCAGTTAAGA CAGGATTCCA GGTCATGTCT GGTGGCTTCA 
TGCCTCACCT CAGAGTTAGT GAAGCTCAGT GCAGAGGAAG TGCCTGAGCC CGCTGAGTGC 
AAGTCAGAGC AAAGCCCAGA AAGCAGAACC CAGGAACCCC AAGGCACCCA ACTCGCACCA 
AGAGCTGCTG ATGAGAGAGA GACACAGAAG CACCTCTGGG GCATTTCTGT CCAGGCAGGA 
AACCAAACCA GTAATTACAC ATCAAAGTAT GTGCTCAGCG AGGAAAGCAA GTCACCTACC 
AGGGCCAAGT TCCCACGGCA GCCTAGCAGT GAGGGGACCC AGGTGTGGAG TGGAGACTTG 
ATGGGCTGCT TGGAAGTGAG TGACAGTTCA GATGCCCCTG AGACCACCCA GAAATCAAGC 
GCAATAGACA CTTCAAAGGC AGCCGAAGAA GCCATGGTTC TAGACCCCAA CTACAGGGAA 
CAGGCTTTGC AAGGTCTTTC AGAATTCAAG GCAGCCACGG TGTCTCACTG CGGCCCCGGG 
GCTGAGGAGG GTGAACAGGG GCCTGGGGGT GCCGGGGGCC GGCAGCTGGA GCCCAAAGCA 
GGCGGCGAGG CCTCGAGGGG CAGGGGCGCC CTCATCATTG TAGCTGTGGA GCAGAAAGGT 
CTTCAGGCCA GCAGGAGCAA TGCGGGGCCT GTTCCAGAAA CACTGCCCCG TGACTTTCCT 
AAGGAAAGAC CAGAATCTCC CTTGAGCACT GGCGAGACCC CCTGTGAGTC TCCCACTAGG 
GGGAAAACAC CAGCCGGTAA TGAGTGTGAG TTGCCAGCAG CCCCCATACA GGGAGCTGGC 
GATGGGGCTC TTCAGCGGGT GGCCCAGGCC GCAGAGCTTG GGAGAGTGCT GGTCCCCCAA 
GCTGCTTCGG AAGAGACGCC GAGCACAGAG GAGCCCCCGG GAGAGAGACT GCGTGGGGAG 
AGCCGGAGCT CCGGGTCAGG GGAGCGTGGC CCGGAGGAGG CCCCCGAAGG CGGTGCTGCA 
GCAGCCCGGG GCCAGCGCCC CCGCGTCCCC GCCTTGGAGC CGCCCCAGCC GCCACGCGGG 
CTCCGCAAGG GCGCGCAGGA GCCTGGGAAG CGCCCGACGT TTTCCAAGGT GACCTCCTTC 
AGGAAGGGCA GGCCCTTGGC CACTGAGAGC CCAGGAGGGG TCCCGGCCCC GACCACCGAG 
GGTCGCCGCT GGGGCTCTTC AGGCCCCGAG GGGCTCCCCA GGGAGAATCC GCCCGCTGCG 
GCCGGTCGGG ACGCACCGCC TCTGCACCAC GGGGACGCCA GCGCCTGGCC CGAGTTTGTC 
CCGCAGGCTG CAGGCGACAG GACTGCAGGG CCGGCAGGAG CGGGGCACAC GGGGACCTCA 
GGGGATCTTG GTAGCCGAGG GCCTTCCTCT GAGAGCTGCA ACGCAAAGAG ACTCAAAACA 
ACGGAGAAAA AACTCAGGGC AAGGTTGGCC TTGGCTCATA AGACCTTTTC AAACTTTATT 
GAGTCAATAG TTCTAGAGAA AGAGAACACC CATGAACGTT CCCCAAGTTC TCCCAAGGGC 
GAGAAGGAGA AGAGCAGGCT GCGCCAGGGT TCCTGGCGGG CGTTTCTGAA AAGCAAAGAT 
GCCGGAAGCC CCAAAAAGCC CACCCTAGTG AGTCTGCCTC TAGGACCCGA AGTTCTCTCC 
CCAGCAGAGA CCGACAGCCA CTGTGAGGAA CGGGCGGAGG ACAAAGAGGG CTATGTTTTT 
AGCGATCACT GGGCACCCCC ACTTGCCTCC ACACCTTTGT CCTCCAGTTT AGTTTCTCCA 
GAACACAGGA GGAAAAGTGA ACCGACCATC AAGTGCACAG CCACCCAGGA AGGCGGTAGG 
TACCTACCTT CAGGTATCTT TCCGGAAAAG TCCTGGCTGG CGTCCCCCGG CAGCCCTCGG 
GCCCAGCAGG CTGGAATCGC ACACACCCTG CCTTCCAGCT CTGCCTGCTG CCTGGCATAT 
GAAAACCCCG GGACGCCCTG CAGACCCACG AGCCCCAAGC CCCTGAGTCC CAGGCCTAGT 
GCTCAGCGGA TGGGTCTCCA CTACCCCGGG AGGGGTAGCG CCATCTCCAT GGTTTCTCTT 
GGAAGCTACA GCTACGTGGA CAGCAGTTCA GGGGACCCTG AAAGACCCAA GATTCCCAAG 
GGCCAGACCA GTTTCCTGCT TTCTCTGCAG ACGCTAAACC AAGATGAGCA GAAGGAAGAG 
AGCAGGGAAG GAGGCCAGGG TCCGCGCGGC TTGGGCACAG TGCCCTGGCT CAGGGACCTT 
CCTGGGAGTG AGAACCACAT GCCCTGGGAA GAACCAGCAG GTGAGAAGCC CAGTTGCTCT 
CACAGTCAGA AGGCGTTCCA CATGGAGCCT GCCCAGAAGC CCTGCTTCAC CACTGACATG 
GTGACATGGG CCCTCCTCTG CATCTCTGCA GAGACTGTGC GTGGGGAGGC TCCTTCACAG 
CCTAGGGGCA TCCCTCACCG CTCGCCCGTC AGTGTGGATG ACCTGTGGCT GGAGAAGACA 
CAGAGAAAGA AGTTGCAGAA GCAGGCCCAC GTCGAAAGGA GGCTGCACAT AGGGGCAGTG 
CACAAAGATG GAGTCAAGTG CTGGAGAAAG ACGATCATTA CCTCTCCAGA GTCTTTGAAT 
CTCCCTAGAA GAAGCCATCC ACTCTCCCAG AGTGCTCCAA CGGGACTGAA CCACATGGGC 
TGGCCAGAGC ACACACCAGG CACTGCCATG CCTGATGGAG CTCTGGACAC AGCTGTCTGC 
GCTGACGAAG TGGGGAGCGA GGAGGACCTG TATGATGACC TGCACAGCTC CAGCCACCAC 
TACAGCCACC CTGGAGGGGG TGGGGAGCAG CTGGCTATCA ATGAGCTCAT CAGCGATGGC 
AGTGTGGTCT GCGCTGAAGC ACTCTGGGAC CATGTCACCA TGGACGACCA GGAGCTGGGC 
TTCAAAGCTG GGGACGTCAT CGAAGTGATG GATGCCACCA ACAGAGAGTG GTGGTGGGGC 
CGGGTCGCCG ATGGCGAGGG CTGGTTTCCA GCCAGCTTCG TTCGGCTGAG GGTGAATCAG 
GACGAGCCCG CGGATGACGA CGCCCCTCTG GCCGGGAACA GCGGAGCGGA GGACGGCGGG 
GCGGAGGCGC AGAGCAGCAA GGACCAGATG CGGACCAACG TCATCAACGA GATCCTCAGC 
ACTGAGCGGG ACTACATCAA GCACCTGCGC GACATCTGCG AGGGCTACGT CCGGCAGTGC 
CGCAAGCGCG CAGACATGTT CAGCGAGGAG CAGCTGCGTA CCATCTTCGG GAACATCGAG 
GACATCTACC GCTGCCAGAA GGCCTTCGTG AAGGCCCTGG AGCAGAGGTT CAACCGCGAG 
CGCCCACACC TGAGCGAGCT GGGTGCCTGC TTCCTGGAGC ATCAAGCCGA CTTCCAGATC 
TACTCGGAGT ACTGCAATAA CCACCCCAAC GCCTGCGTGG AGCTCTCCCG GCTCACCAAG 
CTCAGCAAGT ACGTGTACTT CTTCGAGGCC TGCCGGCTGC TGCAGAAGAT GATTGACATC 
TCCCTGGATG GCTTCCTGCT GACTCCGGTG CAGAAGATCT GCAAGTACCC TCTGCAGCTG 
GCCGAGCTGC TCAAATACAC GCACCCCCAG CACAGGGACT TCAAGGATGT TGAAGCCGCC 
TTGCATGCCA TGAAGAACGT GGCCCAGCTC ATCAACGAGC GGAAGCGGAG ACTTGAGAAC 
ATCGACAAGA TTGCTCAGTG GCAGAGCTCC ATAGAGGACT GGGAGGGAGA AGATCTCTTG 
GTCAGGAGCT CAGAACTCAT CTACTCGGGG GAGCTGACTC GAGTTACACA GCCTCAAGCC 
AAAAGCCAGC AGCGAATGTT CTTTCTCTTT GACCACCAGC TCATCTACTG TAAGAAGGAC 
CTGCTCCGCC GCGACGTGTT GTACTACAAG GGCCGGCTGG ACATGGACGG CCTGGAGGTG 
GTGGACCTGG AGGACGGGAA GGACAGAGAC CTCCATGTGA GCATCAAGAA CGCCTTCCGG 
CTGCACCGTG GCGCCACAGG GGACAGCCAC CTGCTGTGCA CCAGGAAGCC CGAGCAGAAG 
CAGCGCTGGC TCAAGGCCTT TGCCAGGGAG AGGGAGCAGG TGCAGCTGGA CCAGGAGACA 
GGCTTCTCCA TCACTGAACT GCAGAGGAAG CAGGCCATGC TGAATGCCAG CAAGCAGCAG 
GTCACAGGGA AGCCCAAAGG CCACCCCCCT CTTCAGGCTG CTGCAGGAGG CCAGGCCCAG 
GCCAGAGACC CCAGGCAGCC TGAGCTCTTG GATGTGGTCA GCTCTCCAGG AGCAAGTCCT 
GTGTGGCAAA ACTGCCTCCT ACGGCGGCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509576.1
CDS	39..5651
Translation	MLSVVHFLRSFFKTPEPGAHLPGEGEIEDNQLPTSPAEQVEQGWNQQTDRDDSETLSQQSESGSDTKTDPFESASDTESLSGYLPRGVFHPLRGTPVDIEAPWEYPDVSATGPPQEQHLTSVPGLHAKEELDLSPSLEDDSCKNGWRAFATVAGEQEAGHLWDCATSLERESLLAGVPRHTGCCLQRATDSSGPEPVQGVAVQDLRGLSSVSLQKSRSESYLGIPVVWPFLLWCCELGRSWPHIHNRARALVLPSRGPLDNTAPLGTRTKKESTLGPAGDTELLWSQPHSDVPCQPPLRTSCLLRTNRHHSAPETTGDKNRASPRLNCGHMRALVRAHSIAGFSPECPEDPVGQNVVKSGTHVKEGAKNERDPRIQNIPSPAPTQLSGPIPAFQSGAPHLQGPCKPGGFRLQRASQDTPSAGLLGENQLRQDSRSCLVASCLTSELVKLSAEEVPEPAECKSEQSPESRTQEPQGTQLAPRAADERETQKHLWGISVQAGNQTSNYTSKYVLSEESKSPTRAKFPRQPSSEGTQVWSGDLMGCLEVSDSSDAPETTQKSSAIDTSKAAEEAMVLDPNYREQALQGLSEFKAATVSHCGPGAEEGEQGPGGAGGRQLEPKAGGEASRGRGALIIVAVEQKGLQASRSNAGPVPETLPRDFPKERPESPLSTGETPCESPTRGKTPAGNECELPAAPIQGAGDGALQRVAQAAELGRVLVPQAASEETPSTEEPPGERLRGESRSSGSGERGPEEAPEGGAAAARGQRPRVPALEPPQPPRGLRKGAQEPGKRPTFSKVTSFRKGRPLATESPGGVPAPTTEGRRWGSSGPEGLPRENPPAAAGRDAPPLHHGDASAWPEFVPQAAGDRTAGPAGAGHTGTSGDLGSRGPSSESCNAKRLKTTEKKLRARLALAHKTFSNFIESIVLEKENTHERSPSSPKGEKEKSRLRQGSWRAFLKSKDAGSPKKPTLVSLPLGPEVLSPAETDSHCEERAEDKEGYVFSDHWAPPLASTPLSSSLVSPEHRRKSEPTIKCTATQEGGRYLPSGIFPEKSWLASPGSPRAQQAGIAHTLPSSSACCLAYENPGTPCRPTSPKPLSPRPSAQRMGLHYPGRGSAISMVSLGSYSYVDSSSGDPERPKIPKGQTSFLLSLQTLNQDEQKEESREGGQGPRGLGTVPWLRDLPGSENHMPWEEPAGEKPSCSHSQKAFHMEPAQKPCFTTDMVTWALLCISAETVRGEAPSQPRGIPHRSPVSVDDLWLEKTQRKKLQKQAHVERRLHIGAVHKDGVKCWRKTIITSPESLNLPRRSHPLSQSAPTGLNHMGWPEHTPGTAMPDGALDTAVCADEVGSEEDLYDDLHSSSHHYSHPGGGGEQLAINELISDGSVVCAEALWDHVTMDDQELGFKAGDVIEVMDATNREWWWGRVADGEGWFPASFVRLRVNQDEPADDDAPLAGNSGAEDGGAEAQSSKDQMRTNVINEILSTERDYIKHLRDICEGYVRQCRKRADMFSEEQLRTIFGNIEDIYRCQKAFVKALEQRFNRERPHLSELGACFLEHQADFQIYSEYCNNHPNACVELSRLTKLSKYVYFFEACRLLQKMIDISLDGFLLTPVQKICKYPLQLAELLKYTHPQHRDFKDVEAALHAMKNVAQLINERKRRLENIDKIAQWQSSIEDWEGEDLLVRSSELIYSGELTRVTQPQAKSQQRMFFLFDHQLIYCKKDLLRRDVLYYKGRLDMDGLEVVDLEDGKDRDLHVSIKNAFRLHRGATGDSHLLCTRKPEQKQRWLKAFAREREQVQLDQETGFSITELQRKQAMLNASKQQVTGKPKGHPPLQAAAGGQAQARDPRQPELLDVVSSPGASPVWQNCLLRRL

Target ORF information:

RefSeq Version	XM_011511274.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511274.3

ORF Insert Sequence:

ATGCTCAGCG TCGTGCACTT CCTCCGGAGC TTCTTCAAGA CTCCAGAGCC AGGTGCACAC 
CTGCCAGGTG AAGGTGAGAT TGAAGATAAC CAGCTCCCCA CATCCCCTGC AGAACAAGTG 
GAGCAGGGAT GGAACCAGCA AACAGACCGC GATGATTCTG AAACGCTGTC CCAGCAGAGT 
GAAAGTGGAT CAGACACAAA AACTGACCCC TTTGAAAGTG CCTCTGACAC AGAGTCCTTG 
TCTGGGTACC TCCCGAGGGG AGTCTTCCAC CCTCTAAGAG GTACTCCCGT AGATATAGAA 
GCACCTTGGG AATACCCTGA TGTCTCAGCA ACTGGACCCC CTCAGGAGCA GCATTTGACC 
AGTGTTCCTG GGCTTCATGC AAAGGAAGAA CTCGATTTGT CCCCTAGCTT AGAGGATGAC 
TCTTGCAAAA ATGGGTGGCG AGCCTTTGCC ACAGTTGCTG GAGAACAGGA GGCAGGACAC 
CTCTGGGACT GCGCGACCAG CCTGGAGCGA GAGTCTTTGC TGGCAGGGGT TCCCCGACAC 
ACAGGGTGCT GCTTACAGAG GGCCACAGAC AGCAGTGGTC CTGAGCCAGT ACAGGGGGTG 
GCTGTTCAAG ACCTCAGAGG GCTCTCCAGT GTTTCTCTTC AGAAATCCAG GTCTGAGAGC 
TATCTGGGCA TCCCAGTGGT CTGGCCCTTC CTTCTCTGGT GCTGTGAACT GGGTCGGAGT 
TGGCCACATA TCCACAACAG GGCCAGGGCA CTGGTGCTAC CTAGCAGAGG CCCACTGGAC 
AACACAGCCC CTCTGGGGAC CAGGACAAAG AAGGAAAGTA CTCTAGGCCC TGCAGGGGAC 
ACAGAATTGC TCTGGTCCCA GCCCCACTCG GATGTCCCCT GCCAGCCTCC TTTGAGGACA 
TCTTGCCTCC TACGCACCAA CCGTCACCAT AGTGCCCCAG AAACTACTGG TGACAAGAAC 
AGGGCATCCC CCAGACTCAA CTGTGGGCAC ATGAGGGCAC TGGTCAGGGC CCATTCCATA 
GCAGGGTTTT CACCAGAGTG CCCCGAGGAT CCCGTGGGAC AGAATGTTGT GAAGTCTGGG 
ACCCATGTGA AGGAAGGGGC CAAAAATGAA CGAGATCCAA GAATACAAAA CATCCCTTCC 
CCTGCACCCA CCCAGCTGTC TGGCCCGATT CCTGCTTTTC AGAGTGGGGC TCCCCATCTG 
CAGGGTCCCT GCAAGCCTGG TGGGTTTCGC TTGCAAAGGG CCTCTCAGGA CACTCCTTCT 
GCAGGTCTCC TGGGGGAAAA CCAGTTAAGA CAGGATTCCA GGTCATGTCT GGTGGCTTCA 
TGCCTCACCT CAGAGTTAGT GAAGCTCAGT GCAGAGGAAG TGCCTGAGCC CGCTGAGTGC 
AAGTCAGAGC AAAGCCCAGA AAGCAGAACC CAGGAACCCC AAGGCACCCA ACTCGCACCA 
AGAGCTGCTG ATGAGAGAGA GACACAGAAG CACCTCTGGG GCATTTCTGT CCAGGCAGGA 
AACCAAACCA GTAATTACAC ATCAAAGTAT GTGCTCAGCG AGGAAAGCAA GTCACCTACC 
AGGGCCAAGT TCCCACGGCA GCCTAGCAGT GAGGGGACCC AGGTGTGGAG TGGAGACTTG 
ATGGGCTGCT TGGAAGTGAG TGACAGTTCA GATGCCCCTG AGACCACCCA GAAATCAAGC 
GCAATAGACA CTTCAAAGGC AGCCGAAGAA GCCATGGTTC TAGACCCCAA CTACAGGGAA 
CAGGCTTTGC AAGGTCTTTC AGAATTCAAG GCAGCCACGG TGTCTCACTG CGGCCCCGGG 
GCTGAGGAGG GTGAACAGGG GCCTGGGGGT GCCGGGGGCC GGCAGCTGGA GCCCAAAGCA 
GGCGGCGAGG CCTCGAGGGG CAGGGGCGCC CTCATCATTG TAGCTGTGGA GCAGAAAGGT 
CTTCAGGCCA GCAGGAGCAA TGCGGGGCCT GTTCCAGAAA CACTGCCCCG TGACTTTCCT 
AAGGAAAGAC CAGAATCTCC CTTGAGCACT GGCGAGACCC CCTGTGAGTC TCCCACTAGG 
GGGAAAACAC CAGCCGGTAA TGAGTGTGAG TTGCCAGCAG CCCCCATACA GGGAGCTGGC 
GATGGGGCTC TTCAGCGGGT GGCCCAGGCC GCAGAGCTTG GGAGAGTGCT GGTCCCCCAA 
GCTGCTTCGG AAGAGACGCC GAGCACAGAG GAGCCCCCGG GAGAGAGACT GCGTGGGGAG 
AGCCGGAGCT CCGGGTCAGG GGAGCGTGGC CCGGAGGAGG CCCCCGAAGG CGGTGCTGCA 
GCAGCCCGGG GCCAGCGCCC CCGCGTCCCC GCCTTGGAGC CGCCCCAGCC GCCACGCGGG 
CTCCGCAAGG GCGCGCAGGA GCCTGGGAAG CGCCCGACGT TTTCCAAGGT GACCTCCTTC 
AGGAAGGGCA GGCCCTTGGC CACTGAGAGC CCAGGAGGGG TCCCGGCCCC GACCACCGAG 
GGTCGCCGCT GGGGCTCTTC AGGCCCCGAG GGGCTCCCCA GGGAGAATCC GCCCGCTGCG 
GCCGGTCGGG ACGCACCGCC TCTGCACCAC GGGGACGCCA GCGCCTGGCC CGAGTTTGTC 
CCGCAGGCTG CAGGCGACAG GACTGCAGGG CCGGCAGGAG CGGGGCACAC GGGGACCTCA 
GGGGATCTTG GTAGCCGAGG GCCTTCCTCT GAGAGCTGCA ACGCAAAGAG ACTCAAAACA 
ACGGAGAAAA AACTCAGGGC AAGGTTGGCC TTGGCTCATA AGACCTTTTC AAACTTTATT 
GAGTCAATAG TTCTAGAGAA AGAGAACACC CATGAACGTT CCCCAAGTTC TCCCAAGGGC 
GAGAAGGAGA AGAGCAGGCT GCGCCAGGGT TCCTGGCGGG CGTTTCTGAA AAGCAAAGAT 
GCCGGAAGCC CCAAAAAGCC CACCCTAGTG AGTCTGCCTC TAGGACCCGA AGTTCTCTCC 
CCAGCAGAGA CCGACAGCCA CTGTGAGGAA CGGGCGGAGG ACAAAGAGGG CTATGTTTTT 
AGCGATCACT GGGCACCCCC ACTTGCCTCC ACACCTTTGT CCTCCAGTTT AGTTTCTCCA 
GAACACAGGA GGAAAAGTGA ACCGACCATC AAGTGCACAG CCACCCAGGA AGGCGGTAGG 
TACCTACCTT CAGGTATCTT TCCGGAAAAG TCCTGGCTGG CGTCCCCCGG CAGCCCTCGG 
GCCCAGCAGG CTGGAATCGC ACACACCCTG CCTTCCAGCT CTGCCTGCTG CCTGGCATAT 
GAAAACCCCG GGACGCCCTG CAGACCCACG AGCCCCAAGC CCCTGAGTCC CAGGCCTAGT 
GCTCAGCGGA TGGGTCTCCA CTACCCCGGG AGGGGTAGCG CCATCTCCAT GGTTTCTCTT 
GGAAGCTACA GCTACGTGGA CAGCAGTTCA GGGGACCCTG AAAGACCCAA GATTCCCAAG 
GGCCAGACCA GTTTCCTGCT TTCTCTGCAG ACGCTAAACC AAGATGAGCA GAAGGAAGAG 
AGCAGGGAAG GAGGCCAGGG TCCGCGCGGC TTGGGCACAG TGCCCTGGCT CAGGGACCTT 
CCTGGGAGTG AGAACCACAT GCCCTGGGAA GAACCAGCAG GTGAGAAGCC CAGTTGCTCT 
CACAGTCAGA AGGCGTTCCA CATGGAGCCT GCCCAGAAGC CCTGCTTCAC CACTGACATG 
GTGACATGGG CCCTCCTCTG CATCTCTGCA GAGACTGTGC GTGGGGAGGC TCCTTCACAG 
CCTAGGGGCA TCCCTCACCG CTCGCCCGTC AGTGTGGATG ACCTGTGGCT GGAGAAGACA 
CAGAGAAAGA AGTTGCAGAA GCAGGCCCAC GTCGAAAGGA GGCTGCACAT AGGGGCAGTG 
CACAAAGATG GAGTCAAGTG CTGGAGAAAG ACGATCATTA CCTCTCCAGA GTCTTTGAAT 
CTCCCTAGAA GAAGCCATCC ACTCTCCCAG AGTGCTCCAA CGGGACTGAA CCACATGGGC 
TGGCCAGAGC ACACACCAGG CACTGCCATG CCTGATGGAG CTCTGGACAC AGCTGTCTGC 
GCTGACGAAG TGGGGAGCGA GGAGGACCTG TATGATGACC TGCACAGCTC CAGCCACCAC 
TACAGCCACC CTGGAGGGGG TGGGGAGCAG CTGGCTATCA ATGAGCTCAT CAGCGATGGC 
AGTGTGGTCT GCGCTGAAGC ACTCTGGGAC CATGTCACCA TGGACGACCA GGAGCTGGGC 
TTCAAAGCTG GGGACGTCAT CGAAGTGATG GATGCCACCA ACAGAGAGTG GTGGTGGGGC 
CGGGTCGCCG ATGGCGAGGG CTGGTTTCCA GCCAGCTTCG TTCGGCTGAG GGTGAATCAG 
GACGAGCCCG CGGATGACGA CGCCCCTCTG GCCGGGAACA GCGGAGCGGA GGACGGCGGG 
GCGGAGGCGC AGAGCAGCAA GGACCAGATG CGGACCAACG TCATCAACGA GATCCTCAGC 
ACTGAGCGGG ACTACATCAA GCACCTGCGC GACATCTGCG AGGGCTACGT CCGGCAGTGC 
CGCAAGCGCG CAGACATGTT CAGCGAGGAG CAGCTGCGTA CCATCTTCGG GAACATCGAG 
GACATCTACC GCTGCCAGAA GGCCTTCGTG AAGGCCCTGG AGCAGAGGTT CAACCGCGAG 
CGCCCACACC TGAGCGAGCT GGGTGCCTGC TTCCTGGAGC ATCAAGCCGA CTTCCAGATC 
TACTCGGAGT ACTGCAATAA CCACCCCAAC GCCTGCGTGG AGCTCTCCCG GCTCACCAAG 
CTCAGCAAGT ACGTGTACTT CTTCGAGGCC TGCCGGCTGC TGCAGAAGAT GATTGACATC 
TCCCTGGATG GCTTCCTGCT GACTCCGGTG CAGAAGATCT GCAAGTACCC TCTGCAGCTG 
GCCGAGCTGC TCAAATACAC GCACCCCCAG CACAGGGACT TCAAGGATGT TGAAGCCGCC 
TTGCATGCCA TGAAGAACGT GGCCCAGCTC ATCAACGAGC GGAAGCGGAG ACTTGAGAAC 
ATCGACAAGA TTGCTCAGTG GCAGAGCTCC ATAGAGGACT GGGAGGGAGA AGATCTCTTG 
GTCAGGAGCT CAGAACTCAT CTACTCGGGG GAGCTGACTC GAGTTACACA GCCTCAAGCC 
AAAAGCCAGC AGCGAATGTT CTTTCTCTTT GACCACCAGC TCATCTACTG TAAGAAGGAC 
CTGCTCCGCC GCGACGTGTT GTACTACAAG GGCCGGCTGG ACATGGACGG CCTGGAGGTG 
GTGGACCTGG AGGACGGGAA GGACAGAGAC CTCCATGTGA GCATCAAGAA CGCCTTCCGG 
CTGCACCGTG GCGCCACAGG GGACAGCCAC CTGCTGTGCA CCAGGAAGCC CGAGCAGAAG 
CAGCGCTGGC TCAAGGCCTT TGCCAGGGAG AGGGAGCAGG TGCAGCTGGA CCAGGAGACA 
GGCTTCTCCA TCACTGAACT GCAGAGGAAG CAGGCCATGC TGAATGCCAG CAAGCAGCAG 
GTCACAGGGA AGCCCAAAGG CCACCCCCCT CTTCAGGCTG CTGCAGGAGG CCAGGCCCAG 
GCCAGAGACC CCAGGCAGCC TGAGCTCTTG GATGTGGTCA GCTCTCCAGG AGCAAGTCCT 
GTGTGGCAAA ACTGCCTCCT ACGGCGGCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF4 ( XM_017004231.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016859720.1 Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X4, mRNA.

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CloneID	OHu89522
Clone ID Related Accession (Same CDS sequence)	XM_017004231.1
Accession Version	XM_017004231.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCAGCG TCGTGCACTT CCTCCGGAGC TTCTTCAAGA CTCCAGAGCC AGGTGCACAC 
CTGCCAGGTG AAGGTGAGAT TGAAGATAAC CAGCTCCCCA CATCCCCTGC AGAACAAGTG 
GAGCAGGGAT GGAACCAGCA AACAGACCGC GATGATTCTG AAACGCTGTC CCAGCAGAGT 
GAAAGTGGAT CAGACACAAA AACTGACCCC TTTGAAAGTG CCTCTGACAC AGAGTCCTTG 
TCTGGGTACC TCCCGAGGGG AGTCTTCCAC CCTCTAAGAG GTACTCCCGT AGATATAGAA 
GCACCTTGGG AATACCCTGA TGTCTCAGCA ACTGGACCCC CTCAGGAGCA GCATTTGACC 
AGTGTTCCTG GGCTTCATGC AAAGGAAGAA CTCGATTTGT CCCCTAGCTT AGAGGATGAC 
TCTTGCAAAA ATGGGTGGCG AGCCTTTGCC ACAGTTGCTG GAGAACAGGA GGCAGGACAC 
CTCTGGGACT GCGCGACCAG CCTGGAGCGA GAGTCTTTGC TGGCAGGGGT TCCCCGACAC 
ACAGGGTGCT GCTTACAGAG GGCCACAGAC AGCAGTGGTC CTGAGCCAGT ACAGGGGGTG 
GCTGTTCAAG ACCTCAGAGG GCTCTCCAGT GTTTCTCTTC AGAAATCCAG GTCTGAGAGC 
TATCTGGGCA TCCCAGTGGT CTGGCCCTTC CTTCTCTGGT GCTGTGAACT GGGTCGGAGT 
TGGCCACATA TCCACAACAG GGCCAGGGCA CTGGTGCTAC CTAGCAGAGG CCCACTGGAC 
AACACAGCCC CTCTGGGGAC CAGGACAAAG AAGGAAAGTA CTCTAGGCCC TGCAGGGGAC 
ACAGAATTGC TCTGGTCCCA GCCCCACTCG GATGTCCCCT GCCAGCCTCC TTTGAGGACA 
TCTTGCCTCC TACGCACCAA CCGTCACCAT AGTGCCCCAG AAACTACTGG TGACAAGAAC 
AGGGCATCCC CCAGACTCAA CTGTGGGCAC ATGAGGGCAC TGGTCAGGGC CCATTCCATA 
GCAGGGTTTT CACCAGAGTG CCCCGAGGAT CCCGTGGGAC AGAATGTTGT GAAGTCTGGG 
ACCCATGTGA AGGAAGGGGC CAAAAATGAA CGAGATCCAA GAATACAAAA CATCCCTTCC 
CCTGCACCCA CCCAGCTGTC TGGCCCGATT CCTGCTTTTC AGAGTGGGGC TCCCCATCTG 
CAGGGTCCCT GCAAGCCTGG TGGGTTTCGC TTGCAAAGGG CCTCTCAGGA CACTCCTTCT 
GCAGGTCTCC TGGGGGAAAA CCAGTTAAGA CAGGATTCCA GGTCATGTCT GGTGGCTTCA 
TGCCTCACCT CAGAGTTAGT GAAGCTCAGT GCAGAGGAAG TGCCTGAGCC CGCTGAGTGC 
AAGTCAGAGC AAAGCCCAGA AAGCAGAACC CAGGAACCCC AAGGCACCCA ACTCGCACCA 
AGAGCTGCTG ATGAGAGAGA GACACAGAAG CACCTCTGGG GCATTTCTGT CCAGGCAGGA 
AACCAAACCA GTAATTACAC ATCAAAGTAT GTGCTCAGCG AGGAAAGCAA GTCACCTACC 
AGGGCCAAGT TCCCACGGCA GCCTAGCAGT GAGGGGACCC AGGTGTGGAG TGGAGACTTG 
ATGGGCTGCT TGGAAGTGAG TGACAGTTCA GATGCCCCTG AGACCACCCA GAAATCAAGC 
GCAATAGACA CTTCAAAGGC AGCCGAAGAA GCCATGGTTC TAGACCCCAA CTACAGGGAA 
CAGGCTTTGC AAGGTCTTTC AGAATTCAAG GCAGCCACGG TGTCTCACTG CGGCCCCGGG 
GCTGAGGAGG GTGAACAGGG GCCTGGGGGT GCCGGGGGCC GGCAGCTGGA GCCCAAAGCA 
GGCGGCGAGG CCTCGAGGGG CAGGGGCGCC CTCATCATTG TAGCTGTGGA GCAGAAAGGT 
CTTCAGGCCA GCAGGAGCAA TGCGGGGCCT GTTCCAGAAA CACTGCCCCG TGACTTTCCT 
AAGGAAAGAC CAGAATCTCC CTTGAGCACT GGCGAGACCC CCTGTGAGTC TCCCACTAGG 
GGGAAAACAC CAGCCGGTAA TGAGTGTGAG TTGCCAGCAG CCCCCATACA GGGAGCTGGC 
GATGGGGCTC TTCAGCGGGT GGCCCAGGCC GCAGAGCTTG GGAGAGTGCT GGTCCCCCAA 
GCTGCTTCGG AAGAGACGCC GAGCACAGAG GAGCCCCCGG GAGAGAGACT GCGTGGGGAG 
AGCCGGAGCT CCGGGTCAGG GGAGCGTGGC CCGGAGGAGG CCCCCGAAGG CGGTGCTGCA 
GCAGCCCGGG GCCAGCGCCC CCGCGTCCCC GCCTTGGAGC CGCCCCAGCC GCCACGCGGG 
CTCCGCAAGG GCGCGCAGGA GCCTGGGAAG CGCCCGACGT TTTCCAAGGT GACCTCCTTC 
AGGAAGGGCA GGCCCTTGGC CACTGAGAGC CCAGGAGGGG TCCCGGCCCC GACCACCGAG 
GGTCGCCGCT GGGGCTCTTC AGGCCCCGAG GGGCTCCCCA GGGAGAATCC GCCCGCTGCG 
GCCGGTCGGG ACGCACCGCC TCTGCACCAC GGGGACGCCA GCGCCTGGCC CGAGTTTGTC 
CCGCAGGCTG CAGGCGACAG GACTGCAGGG CCGGCAGGAG CGGGGCACAC GGGGACCTCA 
GGGGATCTTG GTAGCCGAGG GCCTTCCTCT GAGAGCTGCA ACGCAAAGAG ACTCAAAACA 
ACGGAGAAAA AACTCAGGGC AAGGTTGGCC TTGGCTCATA AGACCTTTTC AAACTTTATT 
GAGTCAATAG TTCTAGAGAA AGAGAACACC CATGAACGTT CCCCAAGTTC TCCCAAGGGC 
GAGAAGGAGA AGAGCAGGCT GCGCCAGGGT TCCTGGCGGG CGTTTCTGAA AAGCAAAGAT 
GCCGGAAGCC CCAAAAAGCC CACCCTAGTG AGTCTGCCTC TAGGACCCGA AGTTCTCTCC 
CCAGCAGAGA CCGACAGCCA CTGTGAGGAA CGGGCGGAGG ACAAAGAGGG CTATGTTTTT 
AGCGATCACT GGGCACCCCC ACTTGCCTCC ACACCTTTGT CCTCCAGTTT AGTTTCTCCA 
GAACACAGGA GGAAAAGTGA ACCGACCATC AAGTGCACAG CCACCCAGGA AGGCGGTAGG 
TACCTACCTT CAGGTATCTT TCCGGAAAAG TCCTGGCTGG CGTCCCCCGG CAGCCCTCGG 
GCCCAGCAGG CTGGAATCGC ACACACCCTG CCTTCCAGCT CTGCCTGCTG CCTGGCATAT 
GAAAACCCCG GGACGCCCTG CAGACCCACG AGCCCCAAGC CCCTGAGTCC CAGGCCTAGT 
GCTCAGCGGA TGGGTCTCCA CTACCCCGGG AGGGGTAGCG CCATCTCCAT GGTTTCTCTT 
GGAAGCTACA GCTACGTGGA CAGCAGTTCA GGGGACCCTG AAAGACCCAA GATTCCCAAG 
GGCCAGACCA GTTTCCTGCT TTCTCTGCAG ACGCTAAACC AAGATGAGCA GAAGGAAGAG 
AGCAGGGAAG GAGGCCAGGG TCCGCGCGGC TTGGGCACAG TGCCCTGGCT CAGGGACCTT 
CCTGGGAGTG AGAACCACAT GCCCTGGGAA GAACCAGCAG GTGAGAAGCC CAGTTGCTCT 
CACAGTCAGA AGGCGTTCCA CATGGAGCCT GCCCAGAAGC CCTGCTTCAC CACTGACATG 
GTGACATGGG CCCTCCTCTG CATCTCTGCA GAGACTGTGC GTGGGGAGGC TCCTTCACAG 
CCTAGGGGCA TCCCTCACCG CTCGCCCGTC AGTGTGGATG ACCTGTGGCT GGAGAAGACA 
CAGAGAAAGA AGTTGCAGAA GCAGGCCCAC GTCGAAAGGA GGCTGCACAT AGGGGCAGTG 
CACAAAGATG GAGTCAAGTG CTGGAGAAAG ACGATCATTA CCTCTCCAGA GTCTTTGAAT 
CTCCCTAGAA GAAGCCATCC ACTCTCCCAG AGTGCTCCAA CGGGACTGAA CCACATGGGC 
TGGCCAGAGC ACACACCAGG CACTGCCATG CCTGATGGAG CTCTGGACAC AGCTGTCTGC 
GCTGACGAAG TGGGGAGCGA GGAGGACCTG TATGATGACC TGCACAGCTC CAGCCACCAC 
TACAGCCACC CTGGAGGGGG TGGGGAGCAG CTGGCTATCA ATGAGCTCAT CAGCGATGGC 
AGTGTGGTCT GCGCTGAAGC ACTCTGGGAC CATGTCACCA TGGACGACCA GGAGCTGGGC 
TTCAAAGCTG GGGACGTCAT CGAAGTGATG GATGCCACCA ACAGAGAGTG GTGGTGGGGC 
CGGGTCGCCG ATGGCGAGGG CTGGTTTCCA GCCAGCTTCG TTCGGCCCCG GGAGTGGGAA 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016859720.1
CDS	39..4364
Translation	MLSVVHFLRSFFKTPEPGAHLPGEGEIEDNQLPTSPAEQVEQGWNQQTDRDDSETLSQQSESGSDTKTDPFESASDTESLSGYLPRGVFHPLRGTPVDIEAPWEYPDVSATGPPQEQHLTSVPGLHAKEELDLSPSLEDDSCKNGWRAFATVAGEQEAGHLWDCATSLERESLLAGVPRHTGCCLQRATDSSGPEPVQGVAVQDLRGLSSVSLQKSRSESYLGIPVVWPFLLWCCELGRSWPHIHNRARALVLPSRGPLDNTAPLGTRTKKESTLGPAGDTELLWSQPHSDVPCQPPLRTSCLLRTNRHHSAPETTGDKNRASPRLNCGHMRALVRAHSIAGFSPECPEDPVGQNVVKSGTHVKEGAKNERDPRIQNIPSPAPTQLSGPIPAFQSGAPHLQGPCKPGGFRLQRASQDTPSAGLLGENQLRQDSRSCLVASCLTSELVKLSAEEVPEPAECKSEQSPESRTQEPQGTQLAPRAADERETQKHLWGISVQAGNQTSNYTSKYVLSEESKSPTRAKFPRQPSSEGTQVWSGDLMGCLEVSDSSDAPETTQKSSAIDTSKAAEEAMVLDPNYREQALQGLSEFKAATVSHCGPGAEEGEQGPGGAGGRQLEPKAGGEASRGRGALIIVAVEQKGLQASRSNAGPVPETLPRDFPKERPESPLSTGETPCESPTRGKTPAGNECELPAAPIQGAGDGALQRVAQAAELGRVLVPQAASEETPSTEEPPGERLRGESRSSGSGERGPEEAPEGGAAAARGQRPRVPALEPPQPPRGLRKGAQEPGKRPTFSKVTSFRKGRPLATESPGGVPAPTTEGRRWGSSGPEGLPRENPPAAAGRDAPPLHHGDASAWPEFVPQAAGDRTAGPAGAGHTGTSGDLGSRGPSSESCNAKRLKTTEKKLRARLALAHKTFSNFIESIVLEKENTHERSPSSPKGEKEKSRLRQGSWRAFLKSKDAGSPKKPTLVSLPLGPEVLSPAETDSHCEERAEDKEGYVFSDHWAPPLASTPLSSSLVSPEHRRKSEPTIKCTATQEGGRYLPSGIFPEKSWLASPGSPRAQQAGIAHTLPSSSACCLAYENPGTPCRPTSPKPLSPRPSAQRMGLHYPGRGSAISMVSLGSYSYVDSSSGDPERPKIPKGQTSFLLSLQTLNQDEQKEESREGGQGPRGLGTVPWLRDLPGSENHMPWEEPAGEKPSCSHSQKAFHMEPAQKPCFTTDMVTWALLCISAETVRGEAPSQPRGIPHRSPVSVDDLWLEKTQRKKLQKQAHVERRLHIGAVHKDGVKCWRKTIITSPESLNLPRRSHPLSQSAPTGLNHMGWPEHTPGTAMPDGALDTAVCADEVGSEEDLYDDLHSSSHHYSHPGGGGEQLAINELISDGSVVCAEALWDHVTMDDQELGFKAGDVIEVMDATNREWWWGRVADGEGWFPASFVRPREWEP

Target ORF information:

RefSeq Version	XM_017004231.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 4 (ARHGEF4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004231.1

ORF Insert Sequence:

ATGCTCAGCG TCGTGCACTT CCTCCGGAGC TTCTTCAAGA CTCCAGAGCC AGGTGCACAC 
CTGCCAGGTG AAGGTGAGAT TGAAGATAAC CAGCTCCCCA CATCCCCTGC AGAACAAGTG 
GAGCAGGGAT GGAACCAGCA AACAGACCGC GATGATTCTG AAACGCTGTC CCAGCAGAGT 
GAAAGTGGAT CAGACACAAA AACTGACCCC TTTGAAAGTG CCTCTGACAC AGAGTCCTTG 
TCTGGGTACC TCCCGAGGGG AGTCTTCCAC CCTCTAAGAG GTACTCCCGT AGATATAGAA 
GCACCTTGGG AATACCCTGA TGTCTCAGCA ACTGGACCCC CTCAGGAGCA GCATTTGACC 
AGTGTTCCTG GGCTTCATGC AAAGGAAGAA CTCGATTTGT CCCCTAGCTT AGAGGATGAC 
TCTTGCAAAA ATGGGTGGCG AGCCTTTGCC ACAGTTGCTG GAGAACAGGA GGCAGGACAC 
CTCTGGGACT GCGCGACCAG CCTGGAGCGA GAGTCTTTGC TGGCAGGGGT TCCCCGACAC 
ACAGGGTGCT GCTTACAGAG GGCCACAGAC AGCAGTGGTC CTGAGCCAGT ACAGGGGGTG 
GCTGTTCAAG ACCTCAGAGG GCTCTCCAGT GTTTCTCTTC AGAAATCCAG GTCTGAGAGC 
TATCTGGGCA TCCCAGTGGT CTGGCCCTTC CTTCTCTGGT GCTGTGAACT GGGTCGGAGT 
TGGCCACATA TCCACAACAG GGCCAGGGCA CTGGTGCTAC CTAGCAGAGG CCCACTGGAC 
AACACAGCCC CTCTGGGGAC CAGGACAAAG AAGGAAAGTA CTCTAGGCCC TGCAGGGGAC 
ACAGAATTGC TCTGGTCCCA GCCCCACTCG GATGTCCCCT GCCAGCCTCC TTTGAGGACA 
TCTTGCCTCC TACGCACCAA CCGTCACCAT AGTGCCCCAG AAACTACTGG TGACAAGAAC 
AGGGCATCCC CCAGACTCAA CTGTGGGCAC ATGAGGGCAC TGGTCAGGGC CCATTCCATA 
GCAGGGTTTT CACCAGAGTG CCCCGAGGAT CCCGTGGGAC AGAATGTTGT GAAGTCTGGG 
ACCCATGTGA AGGAAGGGGC CAAAAATGAA CGAGATCCAA GAATACAAAA CATCCCTTCC 
CCTGCACCCA CCCAGCTGTC TGGCCCGATT CCTGCTTTTC AGAGTGGGGC TCCCCATCTG 
CAGGGTCCCT GCAAGCCTGG TGGGTTTCGC TTGCAAAGGG CCTCTCAGGA CACTCCTTCT 
GCAGGTCTCC TGGGGGAAAA CCAGTTAAGA CAGGATTCCA GGTCATGTCT GGTGGCTTCA 
TGCCTCACCT CAGAGTTAGT GAAGCTCAGT GCAGAGGAAG TGCCTGAGCC CGCTGAGTGC 
AAGTCAGAGC AAAGCCCAGA AAGCAGAACC CAGGAACCCC AAGGCACCCA ACTCGCACCA 
AGAGCTGCTG ATGAGAGAGA GACACAGAAG CACCTCTGGG GCATTTCTGT CCAGGCAGGA 
AACCAAACCA GTAATTACAC ATCAAAGTAT GTGCTCAGCG AGGAAAGCAA GTCACCTACC 
AGGGCCAAGT TCCCACGGCA GCCTAGCAGT GAGGGGACCC AGGTGTGGAG TGGAGACTTG 
ATGGGCTGCT TGGAAGTGAG TGACAGTTCA GATGCCCCTG AGACCACCCA GAAATCAAGC 
GCAATAGACA CTTCAAAGGC AGCCGAAGAA GCCATGGTTC TAGACCCCAA CTACAGGGAA 
CAGGCTTTGC AAGGTCTTTC AGAATTCAAG GCAGCCACGG TGTCTCACTG CGGCCCCGGG 
GCTGAGGAGG GTGAACAGGG GCCTGGGGGT GCCGGGGGCC GGCAGCTGGA GCCCAAAGCA 
GGCGGCGAGG CCTCGAGGGG CAGGGGCGCC CTCATCATTG TAGCTGTGGA GCAGAAAGGT 
CTTCAGGCCA GCAGGAGCAA TGCGGGGCCT GTTCCAGAAA CACTGCCCCG TGACTTTCCT 
AAGGAAAGAC CAGAATCTCC CTTGAGCACT GGCGAGACCC CCTGTGAGTC TCCCACTAGG 
GGGAAAACAC CAGCCGGTAA TGAGTGTGAG TTGCCAGCAG CCCCCATACA GGGAGCTGGC 
GATGGGGCTC TTCAGCGGGT GGCCCAGGCC GCAGAGCTTG GGAGAGTGCT GGTCCCCCAA 
GCTGCTTCGG AAGAGACGCC GAGCACAGAG GAGCCCCCGG GAGAGAGACT GCGTGGGGAG 
AGCCGGAGCT CCGGGTCAGG GGAGCGTGGC CCGGAGGAGG CCCCCGAAGG CGGTGCTGCA 
GCAGCCCGGG GCCAGCGCCC CCGCGTCCCC GCCTTGGAGC CGCCCCAGCC GCCACGCGGG 
CTCCGCAAGG GCGCGCAGGA GCCTGGGAAG CGCCCGACGT TTTCCAAGGT GACCTCCTTC 
AGGAAGGGCA GGCCCTTGGC CACTGAGAGC CCAGGAGGGG TCCCGGCCCC GACCACCGAG 
GGTCGCCGCT GGGGCTCTTC AGGCCCCGAG GGGCTCCCCA GGGAGAATCC GCCCGCTGCG 
GCCGGTCGGG ACGCACCGCC TCTGCACCAC GGGGACGCCA GCGCCTGGCC CGAGTTTGTC 
CCGCAGGCTG CAGGCGACAG GACTGCAGGG CCGGCAGGAG CGGGGCACAC GGGGACCTCA 
GGGGATCTTG GTAGCCGAGG GCCTTCCTCT GAGAGCTGCA ACGCAAAGAG ACTCAAAACA 
ACGGAGAAAA AACTCAGGGC AAGGTTGGCC TTGGCTCATA AGACCTTTTC AAACTTTATT 
GAGTCAATAG TTCTAGAGAA AGAGAACACC CATGAACGTT CCCCAAGTTC TCCCAAGGGC 
GAGAAGGAGA AGAGCAGGCT GCGCCAGGGT TCCTGGCGGG CGTTTCTGAA AAGCAAAGAT 
GCCGGAAGCC CCAAAAAGCC CACCCTAGTG AGTCTGCCTC TAGGACCCGA AGTTCTCTCC 
CCAGCAGAGA CCGACAGCCA CTGTGAGGAA CGGGCGGAGG ACAAAGAGGG CTATGTTTTT 
AGCGATCACT GGGCACCCCC ACTTGCCTCC ACACCTTTGT CCTCCAGTTT AGTTTCTCCA 
GAACACAGGA GGAAAAGTGA ACCGACCATC AAGTGCACAG CCACCCAGGA AGGCGGTAGG 
TACCTACCTT CAGGTATCTT TCCGGAAAAG TCCTGGCTGG CGTCCCCCGG CAGCCCTCGG 
GCCCAGCAGG CTGGAATCGC ACACACCCTG CCTTCCAGCT CTGCCTGCTG CCTGGCATAT 
GAAAACCCCG GGACGCCCTG CAGACCCACG AGCCCCAAGC CCCTGAGTCC CAGGCCTAGT 
GCTCAGCGGA TGGGTCTCCA CTACCCCGGG AGGGGTAGCG CCATCTCCAT GGTTTCTCTT 
GGAAGCTACA GCTACGTGGA CAGCAGTTCA GGGGACCCTG AAAGACCCAA GATTCCCAAG 
GGCCAGACCA GTTTCCTGCT TTCTCTGCAG ACGCTAAACC AAGATGAGCA GAAGGAAGAG 
AGCAGGGAAG GAGGCCAGGG TCCGCGCGGC TTGGGCACAG TGCCCTGGCT CAGGGACCTT 
CCTGGGAGTG AGAACCACAT GCCCTGGGAA GAACCAGCAG GTGAGAAGCC CAGTTGCTCT 
CACAGTCAGA AGGCGTTCCA CATGGAGCCT GCCCAGAAGC CCTGCTTCAC CACTGACATG 
GTGACATGGG CCCTCCTCTG CATCTCTGCA GAGACTGTGC GTGGGGAGGC TCCTTCACAG 
CCTAGGGGCA TCCCTCACCG CTCGCCCGTC AGTGTGGATG ACCTGTGGCT GGAGAAGACA 
CAGAGAAAGA AGTTGCAGAA GCAGGCCCAC GTCGAAAGGA GGCTGCACAT AGGGGCAGTG 
CACAAAGATG GAGTCAAGTG CTGGAGAAAG ACGATCATTA CCTCTCCAGA GTCTTTGAAT 
CTCCCTAGAA GAAGCCATCC ACTCTCCCAG AGTGCTCCAA CGGGACTGAA CCACATGGGC 
TGGCCAGAGC ACACACCAGG CACTGCCATG CCTGATGGAG CTCTGGACAC AGCTGTCTGC 
GCTGACGAAG TGGGGAGCGA GGAGGACCTG TATGATGACC TGCACAGCTC CAGCCACCAC 
TACAGCCACC CTGGAGGGGG TGGGGAGCAG CTGGCTATCA ATGAGCTCAT CAGCGATGGC 
AGTGTGGTCT GCGCTGAAGC ACTCTGGGAC CATGTCACCA TGGACGACCA GGAGCTGGGC 
TTCAAAGCTG GGGACGTCAT CGAAGTGATG GATGCCACCA ACAGAGAGTG GTGGTGGGGC 
CGGGTCGCCG ATGGCGAGGG CTGGTTTCCA GCCAGCTTCG TTCGGCCCCG GGAGTGGGAA 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Copy Number Variations in Candidate Genes and Intergenic Regions Affect Body Mass Index and Abdominal Obesity in Mexican Children.
BioMed research international20172432957(2017)
Ant?nez-Ortiz DL,Flores-Alfaro E,Burguete-Garc?a AI,Bonnefond A,Peralta-Romero J,Froguel P,Espinoza-Rojo M,Cruz M

Quantitative genome-wide methylation analysis of high-grade non-muscle invasive bladder cancer.
Epigenetics11(3)237-46(2016 03)
Kitchen MO,Bryan RT,Emes RD,Glossop JR,Luscombe C,Cheng KK,Zeegers MP,James ND,Devall AJ,Mein CA,Gommersall L,Fryer AA,Farrell WE

Asef mediates HGF protective effects against LPS-induced lung injury and endothelial barrier dysfunction.
American journal of physiology. Lung cellular and molecular physiology308(5)L452-63(2015 Mar)
Meng F,Meliton A,Moldobaeva N,Mutlu G,Kawasaki Y,Akiyama T,Birukova AA

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ARHGEF4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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