ORF » Species Summary » Homo sapiens » NT5E cDNA ORF clone

NT5E cDNA ORF clone, Homo sapiens(human)

The following NT5E gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NT5E cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu18239 NM_002526.4
Latest version!		 Homo sapiens 5'-nucleotidase ecto (NT5E), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$189.50-$265.30
$379.00
OHu13999 NM_001204813.1
Latest version!		 Homo sapiens 5'-nucleotidase ecto (NT5E), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
View Old Accession Versions >>
OHu18239 NM_002526.3 Homo sapiens 5'-nucleotidase ecto (NT5E), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$189.50-$265.30
$379.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    NT5E ( NM_002526.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002517.1 Homo sapiens 5'-nucleotidase ecto (NT5E), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID OHu18239
    Clone ID Related Accession (Same CDS sequence) NM_002526.4 , NM_002526.3
    Accession Version NM_002526.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1725bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-30
    Organism Homo sapiens(human)
    Product 5'-nucleotidase isoform 1 preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA574510.1, X55740.1, BC065937.1, AK075008.1 and BQ019358.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_002526.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.113339.1, X55740.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000257770.8/ ENSP00000257770.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGTGTCCCC GAGCCGCGCG GGCGCCCGCG ACGCTACTCC TCGCCCTGGG CGCGGTGCTG 
    TGGCCTGCGG CTGGCGCCTG GGAGCTTACG ATTTTGCACA CCAACGACGT GCACAGCCGG 
    CTGGAGCAGA CCAGCGAGGA CTCCAGCAAG TGCGTCAACG CCAGCCGCTG CATGGGTGGC 
    GTGGCTCGGC TCTTCACCAA GGTTCAGCAG ATCCGCCGCG CCGAACCCAA CGTGCTGCTG 
    CTGGACGCCG GCGACCAGTA CCAGGGCACT ATCTGGTTCA CCGTGTACAA GGGCGCCGAG 
    GTGGCGCACT TCATGAACGC CCTGCGCTAC GATGCCATGG CACTGGGAAA TCATGAATTT 
    GATAATGGTG TGGAAGGACT GATCGAGCCA CTCCTCAAAG AGGCCAAATT TCCAATTCTG 
    AGTGCAAACA TTAAAGCAAA GGGGCCACTA GCATCTCAAA TATCAGGACT TTATTTGCCA 
    TATAAAGTTC TTCCTGTTGG TGATGAAGTT GTGGGAATCG TTGGATACAC TTCCAAAGAA 
    ACCCCTTTTC TCTCAAATCC AGGGACAAAT TTAGTGTTTG AAGATGAAAT CACTGCATTA 
    CAACCTGAAG TAGATAAGTT AAAAACTCTA AATGTGAACA AAATTATTGC ACTGGGACAT 
    TCGGGTTTTG AAATGGATAA ACTCATCGCT CAGAAAGTGA GGGGTGTGGA CGTCGTGGTG 
    GGAGGACACT CCAACACATT TCTTTACACA GGCAATCCAC CTTCCAAAGA GGTGCCTGCT 
    GGGAAGTACC CATTCATAGT CACTTCTGAT GATGGGCGGA AGGTTCCTGT AGTCCAGGCC 
    TATGCTTTTG GCAAATACCT AGGCTATCTG AAGATCGAGT TTGATGAAAG AGGAAACGTC 
    ATCTCTTCCC ATGGAAATCC CATTCTTCTA AACAGCAGCA TTCCTGAAGA TCCAAGCATA 
    AAAGCAGACA TTAACAAATG GAGGATAAAA TTGGATAATT ATTCTACCCA GGAATTAGGG 
    AAAACAATTG TCTATCTGGA TGGCTCCTCT CAATCATGCC GCTTTAGAGA ATGCAACATG 
    GGCAACCTGA TTTGTGATGC AATGATTAAC AACAACCTGA GACACACGGA TGAAATGTTC 
    TGGAACCACG TATCCATGTG CATTTTAAAT GGAGGTGGTA TCCGGTCGCC CATTGATGAA 
    CGCAACAATG GCACAATTAC CTGGGAGAAC CTGGCTGCTG TATTGCCCTT TGGAGGCACA 
    TTTGACCTAG TCCAGTTAAA AGGTTCCACC CTGAAGAAGG CCTTTGAGCA TAGCGTGCAC 
    CGCTACGGCC AGTCCACTGG AGAGTTCCTG CAGGTGGGCG GAATCCATGT GGTGTATGAT 
    CTTTCCCGAA AACCTGGAGA CAGAGTAGTC AAATTAGATG TTCTTTGCAC CAAGTGTCGA 
    GTGCCCAGTT ATGACCCTCT CAAAATGGAC GAGGTATATA AGGTGATCCT CCCAAACTTC 
    CTGGCCAATG GTGGAGATGG GTTCCAGATG ATAAAAGATG AATTATTAAG ACATGACTCT 
    GGTGACCAAG ATATCAACGT GGTTTCTACA TATATCTCCA AAATGAAAGT AATTTATCCA 
    GCAGTTGAAG GTCGGATCAA GTTTTCCACA GGAAGTCACT GCCATGGAAG CTTTTCTTTA 
    ATATTTCTTT CACTTTGGGC AGTGATCTTT GTTTTATACC AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002517.1
    CDS58..1782
    Translation

    Target ORF information:

    RefSeq Version NM_002526.4
    Organism Homo sapiens(human)
    Definition Homo sapiens 5'-nucleotidase ecto (NT5E), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_002526.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGTGTCCCC GAGCCGCGCG GGCGCCCGCG ACGCTACTCC TCGCCCTGGG CGCGGTGCTG 
    TGGCCTGCGG CTGGCGCCTG GGAGCTTACG ATTTTGCACA CCAACGACGT GCACAGCCGG 
    CTGGAGCAGA CCAGCGAGGA CTCCAGCAAG TGCGTCAACG CCAGCCGCTG CATGGGTGGC 
    GTGGCTCGGC TCTTCACCAA GGTTCAGCAG ATCCGCCGCG CCGAACCCAA CGTGCTGCTG 
    CTGGACGCCG GCGACCAGTA CCAGGGCACT ATCTGGTTCA CCGTGTACAA GGGCGCCGAG 
    GTGGCGCACT TCATGAACGC CCTGCGCTAC GATGCCATGG CACTGGGAAA TCATGAATTT 
    GATAATGGTG TGGAAGGACT GATCGAGCCA CTCCTCAAAG AGGCCAAATT TCCAATTCTG 
    AGTGCAAACA TTAAAGCAAA GGGGCCACTA GCATCTCAAA TATCAGGACT TTATTTGCCA 
    TATAAAGTTC TTCCTGTTGG TGATGAAGTT GTGGGAATCG TTGGATACAC TTCCAAAGAA 
    ACCCCTTTTC TCTCAAATCC AGGGACAAAT TTAGTGTTTG AAGATGAAAT CACTGCATTA 
    CAACCTGAAG TAGATAAGTT AAAAACTCTA AATGTGAACA AAATTATTGC ACTGGGACAT 
    TCGGGTTTTG AAATGGATAA ACTCATCGCT CAGAAAGTGA GGGGTGTGGA CGTCGTGGTG 
    GGAGGACACT CCAACACATT TCTTTACACA GGCAATCCAC CTTCCAAAGA GGTGCCTGCT 
    GGGAAGTACC CATTCATAGT CACTTCTGAT GATGGGCGGA AGGTTCCTGT AGTCCAGGCC 
    TATGCTTTTG GCAAATACCT AGGCTATCTG AAGATCGAGT TTGATGAAAG AGGAAACGTC 
    ATCTCTTCCC ATGGAAATCC CATTCTTCTA AACAGCAGCA TTCCTGAAGA TCCAAGCATA 
    AAAGCAGACA TTAACAAATG GAGGATAAAA TTGGATAATT ATTCTACCCA GGAATTAGGG 
    AAAACAATTG TCTATCTGGA TGGCTCCTCT CAATCATGCC GCTTTAGAGA ATGCAACATG 
    GGCAACCTGA TTTGTGATGC AATGATTAAC AACAACCTGA GACACACGGA TGAAATGTTC 
    TGGAACCACG TATCCATGTG CATTTTAAAT GGAGGTGGTA TCCGGTCGCC CATTGATGAA 
    CGCAACAATG GCACAATTAC CTGGGAGAAC CTGGCTGCTG TATTGCCCTT TGGAGGCACA 
    TTTGACCTAG TCCAGTTAAA AGGTTCCACC CTGAAGAAGG CCTTTGAGCA TAGCGTGCAC 
    CGCTACGGCC AGTCCACTGG AGAGTTCCTG CAGGTGGGCG GAATCCATGT GGTGTATGAT 
    CTTTCCCGAA AACCTGGAGA CAGAGTAGTC AAATTAGATG TTCTTTGCAC CAAGTGTCGA 
    GTGCCCAGTT ATGACCCTCT CAAAATGGAC GAGGTATATA AGGTGATCCT CCCAAACTTC 
    CTGGCCAATG GTGGAGATGG GTTCCAGATG ATAAAAGATG AATTATTAAG ACATGACTCT 
    GGTGACCAAG ATATCAACGT GGTTTCTACA TATATCTCCA AAATGAAAGT AATTTATCCA 
    GCAGTTGAAG GTCGGATCAA GTTTTCCACA GGAAGTCACT GCCATGGAAG CTTTTCTTTA 
    ATATTTCTTT CACTTTGGGC AGTGATCTTT GTTTTATACC AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NT5E ( NM_002526.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002517.1 Homo sapiens 5'-nucleotidase ecto (NT5E), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID OHu18239
    Clone ID Related Accession (Same CDS sequence) NM_002526.4 , NM_002526.3
    Accession Version NM_002526.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1725bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-11-29
    Organism Homo sapiens(human)
    Product 5'-nucleotidase isoform 1 preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA574510.1, X55740.1, BC065937.1, AK075008.1 and BQ019358.1. This sequence is a reference standard in the RefSeqGene project. On Mar 10, 2011 this sequence version replaced NM_002526.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.229160.1, SRR1660807.209152.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGTGTCCCC GAGCCGCGCG GGCGCCCGCG ACGCTACTCC TCGCCCTGGG CGCGGTGCTG 
    TGGCCTGCGG CTGGCGCCTG GGAGCTTACG ATTTTGCACA CCAACGACGT GCACAGCCGG 
    CTGGAGCAGA CCAGCGAGGA CTCCAGCAAG TGCGTCAACG CCAGCCGCTG CATGGGTGGC 
    GTGGCTCGGC TCTTCACCAA GGTTCAGCAG ATCCGCCGCG CCGAACCCAA CGTGCTGCTG 
    CTGGACGCCG GCGACCAGTA CCAGGGCACT ATCTGGTTCA CCGTGTACAA GGGCGCCGAG 
    GTGGCGCACT TCATGAACGC CCTGCGCTAC GATGCCATGG CACTGGGAAA TCATGAATTT 
    GATAATGGTG TGGAAGGACT GATCGAGCCA CTCCTCAAAG AGGCCAAATT TCCAATTCTG 
    AGTGCAAACA TTAAAGCAAA GGGGCCACTA GCATCTCAAA TATCAGGACT TTATTTGCCA 
    TATAAAGTTC TTCCTGTTGG TGATGAAGTT GTGGGAATCG TTGGATACAC TTCCAAAGAA 
    ACCCCTTTTC TCTCAAATCC AGGGACAAAT TTAGTGTTTG AAGATGAAAT CACTGCATTA 
    CAACCTGAAG TAGATAAGTT AAAAACTCTA AATGTGAACA AAATTATTGC ACTGGGACAT 
    TCGGGTTTTG AAATGGATAA ACTCATCGCT CAGAAAGTGA GGGGTGTGGA CGTCGTGGTG 
    GGAGGACACT CCAACACATT TCTTTACACA GGCAATCCAC CTTCCAAAGA GGTGCCTGCT 
    GGGAAGTACC CATTCATAGT CACTTCTGAT GATGGGCGGA AGGTTCCTGT AGTCCAGGCC 
    TATGCTTTTG GCAAATACCT AGGCTATCTG AAGATCGAGT TTGATGAAAG AGGAAACGTC 
    ATCTCTTCCC ATGGAAATCC CATTCTTCTA AACAGCAGCA TTCCTGAAGA TCCAAGCATA 
    AAAGCAGACA TTAACAAATG GAGGATAAAA TTGGATAATT ATTCTACCCA GGAATTAGGG 
    AAAACAATTG TCTATCTGGA TGGCTCCTCT CAATCATGCC GCTTTAGAGA ATGCAACATG 
    GGCAACCTGA TTTGTGATGC AATGATTAAC AACAACCTGA GACACACGGA TGAAATGTTC 
    TGGAACCACG TATCCATGTG CATTTTAAAT GGAGGTGGTA TCCGGTCGCC CATTGATGAA 
    CGCAACAATG GCACAATTAC CTGGGAGAAC CTGGCTGCTG TATTGCCCTT TGGAGGCACA 
    TTTGACCTAG TCCAGTTAAA AGGTTCCACC CTGAAGAAGG CCTTTGAGCA TAGCGTGCAC 
    CGCTACGGCC AGTCCACTGG AGAGTTCCTG CAGGTGGGCG GAATCCATGT GGTGTATGAT 
    CTTTCCCGAA AACCTGGAGA CAGAGTAGTC AAATTAGATG TTCTTTGCAC CAAGTGTCGA 
    GTGCCCAGTT ATGACCCTCT CAAAATGGAC GAGGTATATA AGGTGATCCT CCCAAACTTC 
    CTGGCCAATG GTGGAGATGG GTTCCAGATG ATAAAAGATG AATTATTAAG ACATGACTCT 
    GGTGACCAAG ATATCAACGT GGTTTCTACA TATATCTCCA AAATGAAAGT AATTTATCCA 
    GCAGTTGAAG GTCGGATCAA GTTTTCCACA GGAAGTCACT GCCATGGAAG CTTTTCTTTA 
    ATATTTCTTT CACTTTGGGC AGTGATCTTT GTTTTATACC AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002517.1
    CDS557..2281
    Translation

    Target ORF information:

    RefSeq Version NM_002526.3
    Organism Homo sapiens(human)
    Definition Homo sapiens 5'-nucleotidase ecto (NT5E), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_002526.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGTGTCCCC GAGCCGCGCG GGCGCCCGCG ACGCTACTCC TCGCCCTGGG CGCGGTGCTG 
    TGGCCTGCGG CTGGCGCCTG GGAGCTTACG ATTTTGCACA CCAACGACGT GCACAGCCGG 
    CTGGAGCAGA CCAGCGAGGA CTCCAGCAAG TGCGTCAACG CCAGCCGCTG CATGGGTGGC 
    GTGGCTCGGC TCTTCACCAA GGTTCAGCAG ATCCGCCGCG CCGAACCCAA CGTGCTGCTG 
    CTGGACGCCG GCGACCAGTA CCAGGGCACT ATCTGGTTCA CCGTGTACAA GGGCGCCGAG 
    GTGGCGCACT TCATGAACGC CCTGCGCTAC GATGCCATGG CACTGGGAAA TCATGAATTT 
    GATAATGGTG TGGAAGGACT GATCGAGCCA CTCCTCAAAG AGGCCAAATT TCCAATTCTG 
    AGTGCAAACA TTAAAGCAAA GGGGCCACTA GCATCTCAAA TATCAGGACT TTATTTGCCA 
    TATAAAGTTC TTCCTGTTGG TGATGAAGTT GTGGGAATCG TTGGATACAC TTCCAAAGAA 
    ACCCCTTTTC TCTCAAATCC AGGGACAAAT TTAGTGTTTG AAGATGAAAT CACTGCATTA 
    CAACCTGAAG TAGATAAGTT AAAAACTCTA AATGTGAACA AAATTATTGC ACTGGGACAT 
    TCGGGTTTTG AAATGGATAA ACTCATCGCT CAGAAAGTGA GGGGTGTGGA CGTCGTGGTG 
    GGAGGACACT CCAACACATT TCTTTACACA GGCAATCCAC CTTCCAAAGA GGTGCCTGCT 
    GGGAAGTACC CATTCATAGT CACTTCTGAT GATGGGCGGA AGGTTCCTGT AGTCCAGGCC 
    TATGCTTTTG GCAAATACCT AGGCTATCTG AAGATCGAGT TTGATGAAAG AGGAAACGTC 
    ATCTCTTCCC ATGGAAATCC CATTCTTCTA AACAGCAGCA TTCCTGAAGA TCCAAGCATA 
    AAAGCAGACA TTAACAAATG GAGGATAAAA TTGGATAATT ATTCTACCCA GGAATTAGGG 
    AAAACAATTG TCTATCTGGA TGGCTCCTCT CAATCATGCC GCTTTAGAGA ATGCAACATG 
    GGCAACCTGA TTTGTGATGC AATGATTAAC AACAACCTGA GACACACGGA TGAAATGTTC 
    TGGAACCACG TATCCATGTG CATTTTAAAT GGAGGTGGTA TCCGGTCGCC CATTGATGAA 
    CGCAACAATG GCACAATTAC CTGGGAGAAC CTGGCTGCTG TATTGCCCTT TGGAGGCACA 
    TTTGACCTAG TCCAGTTAAA AGGTTCCACC CTGAAGAAGG CCTTTGAGCA TAGCGTGCAC 
    CGCTACGGCC AGTCCACTGG AGAGTTCCTG CAGGTGGGCG GAATCCATGT GGTGTATGAT 
    CTTTCCCGAA AACCTGGAGA CAGAGTAGTC AAATTAGATG TTCTTTGCAC CAAGTGTCGA 
    GTGCCCAGTT ATGACCCTCT CAAAATGGAC GAGGTATATA AGGTGATCCT CCCAAACTTC 
    CTGGCCAATG GTGGAGATGG GTTCCAGATG ATAAAAGATG AATTATTAAG ACATGACTCT 
    GGTGACCAAG ATATCAACGT GGTTTCTACA TATATCTCCA AAATGAAAGT AATTTATCCA 
    GCAGTTGAAG GTCGGATCAA GTTTTCCACA GGAAGTCACT GCCATGGAAG CTTTTCTTTA 
    ATATTTCTTT CACTTTGGGC AGTGATCTTT GTTTTATACC AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    NT5E ( NM_001204813.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001191742.1 Homo sapiens 5'-nucleotidase ecto (NT5E), transcript variant 2, mRNA.
    Price & Availability↑
    CloneID OHu13999
    Clone ID Related Accession (Same CDS sequence) NM_001204813.1
    Accession Version NM_001204813.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1575bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-23
    Organism Homo sapiens(human)
    Product 5'-nucleotidase isoform 2 preproprotein
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA574510.1, AK075008.1, X55740.1, BC065937.1 and BQ019358.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK075008.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTGTCCCC GAGCCGCGCG GGCGCCCGCG ACGCTACTCC TCGCCCTGGG CGCGGTGCTG 
    TGGCCTGCGG CTGGCGCCTG GGAGCTTACG ATTTTGCACA CCAACGACGT GCACAGCCGG 
    CTGGAGCAGA CCAGCGAGGA CTCCAGCAAG TGCGTCAACG CCAGCCGCTG CATGGGTGGC 
    GTGGCTCGGC TCTTCACCAA GGTTCAGCAG ATCCGCCGCG CCGAACCCAA CGTGCTGCTG 
    CTGGACGCCG GCGACCAGTA CCAGGGCACT ATCTGGTTCA CCGTGTACAA GGGCGCCGAG 
    GTGGCGCACT TCATGAACGC CCTGCGCTAC GATGCCATGG CACTGGGAAA TCATGAATTT 
    GATAATGGTG TGGAAGGACT GATCGAGCCA CTCCTCAAAG AGGCCAAATT TCCAATTCTG 
    AGTGCAAACA TTAAAGCAAA GGGGCCACTA GCATCTCAAA TATCAGGACT TTATTTGCCA 
    TATAAAGTTC TTCCTGTTGG TGATGAAGTT GTGGGAATCG TTGGATACAC TTCCAAAGAA 
    ACCCCTTTTC TCTCAAATCC AGGGACAAAT TTAGTGTTTG AAGATGAAAT CACTGCATTA 
    CAACCTGAAG TAGATAAGTT AAAAACTCTA AATGTGAACA AAATTATTGC ACTGGGACAT 
    TCGGGTTTTG AAATGGATAA ACTCATCGCT CAGAAAGTGA GGGGTGTGGA CGTCGTGGTG 
    GGAGGACACT CCAACACATT TCTTTACACA GGCAATCCAC CTTCCAAAGA GGTGCCTGCT 
    GGGAAGTACC CATTCATAGT CACTTCTGAT GATGGGCGGA AGGTTCCTGT AGTCCAGGCC 
    TATGCTTTTG GCAAATACCT AGGCTATCTG AAGATCGAGT TTGATGAAAG AGGAAACGTC 
    ATCTCTTCCC ATGGAAATCC CATTCTTCTA AACAGCAGCA TTCCTGAAGA TCCAAGCATA 
    AAAGCAGACA TTAACAAATG GAGGATAAAA TTGGATAATT ATTCTACCCA GGAATTAGGG 
    AAAACAATTG TCTATCTGGA TGGCTCCTCT CAATCATGCC GCTTTAGAGA ATGCAACATG 
    GGCAACCTGA TTTGTGATGC AATGATTAAC AACAACCTGA GACACACGGA TGAAATGTTC 
    TGGAACCACG TATCCATGTG CATTTTAAAT GGAGGTGGTA TCCGGTCGCC CATTGATGAA 
    CGCAACAATG GAATCCATGT GGTGTATGAT CTTTCCCGAA AACCTGGAGA CAGAGTAGTC 
    AAATTAGATG TTCTTTGCAC CAAGTGTCGA GTGCCCAGTT ATGACCCTCT CAAAATGGAC 
    GAGGTATATA AGGTGATCCT CCCAAACTTC CTGGCCAATG GTGGAGATGG GTTCCAGATG 
    ATAAAAGATG AATTATTAAG ACATGACTCT GGTGACCAAG ATATCAACGT GGTTTCTACA 
    TATATCTCCA AAATGAAAGT AATTTATCCA GCAGTTGAAG GTCGGATCAA GTTTTCCACA 
    GGAAGTCACT GCCATGGAAG CTTTTCTTTA ATATTTCTTT CACTTTGGGC AGTGATCTTT 
    GTTTTATACC AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001191742.1
    CDS557..2131
    Translation

    Target ORF information:

    RefSeq Version NM_001204813.1
    Organism Homo sapiens(human)
    Definition Homo sapiens 5'-nucleotidase ecto (NT5E), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001204813.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGTGTCCCC GAGCCGCGCG GGCGCCCGCG ACGCTACTCC TCGCCCTGGG CGCGGTGCTG 
    TGGCCTGCGG CTGGCGCCTG GGAGCTTACG ATTTTGCACA CCAACGACGT GCACAGCCGG 
    CTGGAGCAGA CCAGCGAGGA CTCCAGCAAG TGCGTCAACG CCAGCCGCTG CATGGGTGGC 
    GTGGCTCGGC TCTTCACCAA GGTTCAGCAG ATCCGCCGCG CCGAACCCAA CGTGCTGCTG 
    CTGGACGCCG GCGACCAGTA CCAGGGCACT ATCTGGTTCA CCGTGTACAA GGGCGCCGAG 
    GTGGCGCACT TCATGAACGC CCTGCGCTAC GATGCCATGG CACTGGGAAA TCATGAATTT 
    GATAATGGTG TGGAAGGACT GATCGAGCCA CTCCTCAAAG AGGCCAAATT TCCAATTCTG 
    AGTGCAAACA TTAAAGCAAA GGGGCCACTA GCATCTCAAA TATCAGGACT TTATTTGCCA 
    TATAAAGTTC TTCCTGTTGG TGATGAAGTT GTGGGAATCG TTGGATACAC TTCCAAAGAA 
    ACCCCTTTTC TCTCAAATCC AGGGACAAAT TTAGTGTTTG AAGATGAAAT CACTGCATTA 
    CAACCTGAAG TAGATAAGTT AAAAACTCTA AATGTGAACA AAATTATTGC ACTGGGACAT 
    TCGGGTTTTG AAATGGATAA ACTCATCGCT CAGAAAGTGA GGGGTGTGGA CGTCGTGGTG 
    GGAGGACACT CCAACACATT TCTTTACACA GGCAATCCAC CTTCCAAAGA GGTGCCTGCT 
    GGGAAGTACC CATTCATAGT CACTTCTGAT GATGGGCGGA AGGTTCCTGT AGTCCAGGCC 
    TATGCTTTTG GCAAATACCT AGGCTATCTG AAGATCGAGT TTGATGAAAG AGGAAACGTC 
    ATCTCTTCCC ATGGAAATCC CATTCTTCTA AACAGCAGCA TTCCTGAAGA TCCAAGCATA 
    AAAGCAGACA TTAACAAATG GAGGATAAAA TTGGATAATT ATTCTACCCA GGAATTAGGG 
    AAAACAATTG TCTATCTGGA TGGCTCCTCT CAATCATGCC GCTTTAGAGA ATGCAACATG 
    GGCAACCTGA TTTGTGATGC AATGATTAAC AACAACCTGA GACACACGGA TGAAATGTTC 
    TGGAACCACG TATCCATGTG CATTTTAAAT GGAGGTGGTA TCCGGTCGCC CATTGATGAA 
    CGCAACAATG GAATCCATGT GGTGTATGAT CTTTCCCGAA AACCTGGAGA CAGAGTAGTC 
    AAATTAGATG TTCTTTGCAC CAAGTGTCGA GTGCCCAGTT ATGACCCTCT CAAAATGGAC 
    GAGGTATATA AGGTGATCCT CCCAAACTTC CTGGCCAATG GTGGAGATGG GTTCCAGATG 
    ATAAAAGATG AATTATTAAG ACATGACTCT GGTGACCAAG ATATCAACGT GGTTTCTACA 
    TATATCTCCA AAATGAAAGT AATTTATCCA GCAGTTGAAG GTCGGATCAA GTTTTCCACA 
    GGAAGTCACT GCCATGGAAG CTTTTCTTTA ATATTTCTTT CACTTTGGGC AGTGATCTTT 
    GTTTTATACC AATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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