NEK1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_017008250.1	XP_016863739.1	serine/threonine-protein kinase Nek1 isoform X4
XM_017008252.2	XP_016863741.1	serine/threonine-protein kinase Nek1 isoform X5
XM_011532005.2	XP_011530307.1	serine/threonine-protein kinase Nek1 isoform X8
NM_001199397.3	NP_001186326.1	serine/threonine-protein kinase Nek1 isoform 1
NM_001374418.1	NP_001361347.1	serine/threonine-protein kinase Nek1 isoform 1
NM_001199399.1	NP_001186328.1	serine/threonine-protein kinase Nek1 isoform 4
NM_001199398.1	NP_001186327.1	serine/threonine-protein kinase Nek1 isoform 3
NM_001199397.1	NP_001186326.1	serine/threonine-protein kinase Nek1 isoform 1
NM_012224.2	NP_036356.1	serine/threonine-protein kinase Nek1 isoform 2
XM_006714228.1	XP_006714291.1	serine/threonine-protein kinase Nek1 isoform X2
XM_011532004.1	XP_011530306.1	serine/threonine-protein kinase Nek1 isoform X3
NM_001374423.1	NP_001361352.1	serine/threonine-protein kinase Nek1 isoform 8
NM_001199400.2	NP_001186329.1	serine/threonine-protein kinase Nek1 isoform 5
NM_001374422.1	NP_001361351.1	serine/threonine-protein kinase Nek1 isoform 8
NM_001199400.1	NP_001186329.1	serine/threonine-protein kinase Nek1 isoform 5
NM_001199399.2	NP_001186328.1	serine/threonine-protein kinase Nek1 isoform 4
XM_017008251.1	XP_016863740.1	serine/threonine-protein kinase Nek1 isoform X5
NM_001374420.1	NP_001361349.1	serine/threonine-protein kinase Nek1 isoform 6
XM_017008252.1	XP_016863741.1	serine/threonine-protein kinase Nek1 isoform X5
NM_001199397.2	NP_001186326.1	serine/threonine-protein kinase Nek1 isoform 1
XM_011532003.1	XP_011530305.1	serine/threonine-protein kinase Nek1 isoform X1
XM_017008249.1	XP_016863738.1	serine/threonine-protein kinase Nek1 isoform X4
XM_017008253.1	XP_016863742.1	serine/threonine-protein kinase Nek1 isoform X6
XM_017008254.1	XP_016863743.1	serine/threonine-protein kinase Nek1 isoform X7
NM_001374421.1	NP_001361350.1	serine/threonine-protein kinase Nek1 isoform 7
XM_024454065.1	XP_024309833.1	serine/threonine-protein kinase Nek1 isoform X4
NM_001199398.2	NP_001186327.1	serine/threonine-protein kinase Nek1 isoform 3
NM_001374419.1	NP_001361348.1	serine/threonine-protein kinase Nek1 isoform 2
NM_012224.3	NP_036356.1	serine/threonine-protein kinase Nek1 isoform 2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu10748	NM_001374419.1 Latest version!	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu10748	NM_012224.3 Latest version!	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu11534	NM_001199399.2 Latest version!	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu21522	NM_001199397.3 Latest version!	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu21522	NM_001374418.1 Latest version!	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu10748	XM_011532003.1 Latest version!	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu109294	NM_001374422.1 Latest version!	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu109294	NM_001374423.1 Latest version!	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu95207	XM_017008253.1 Latest version!	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$475.30 $679.00
OHu95208	XM_017008254.1 Latest version!	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$475.30 $679.00
OHu59243	XM_011532005.2 Latest version!	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu95205	XM_017008250.1 Latest version!	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$559.30 $799.00
OHu95205	XM_017008249.1 Latest version!	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$559.30 $799.00
OHu95205	XM_024454065.1 Latest version!	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$559.30 $799.00
OHu95206	XM_017008251.1 Latest version!	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$559.30 $799.00
OHu95206	XM_017008252.2 Latest version!	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$559.30 $799.00
OHu109305	NM_001374421.1 Latest version!	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu109306	NM_001374420.1 Latest version!	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu11596	NM_001199398.2 Latest version!	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu11869	NM_001199400.2 Latest version!	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu11869	XM_011532004.1 Latest version!	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu38950	XM_006714228.1 Latest version!	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OHu21522	NM_001199397.1	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu11869	NM_001199400.1	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu11534	NM_001199399.1	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu95206	XM_017008252.1	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$559.30 $799.00
OHu21522	NM_001199397.2	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu11596	NM_001199398.1	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu10748	NM_012224.2	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
Hide Old Accession Versions >>

NEK1 ( NM_012224.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_036356.1 Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu10748
Clone ID Related Accession (Same CDS sequence)	NM_012224.2 , XM_011532003.1 , NM_001374419.1 , NM_012224.3
Accession Version	NM_012224.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA697159.1, AB067488.1, CR933642.1, AK025658.1 and AI984346.1. On or before Aug 31, 2013 this sequence version replaced XM_005263027.1, NM_012224.1. Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB067488.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTGGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG AGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT TCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGTTTA TCTGGCAAGA 
CTGAGGCAAA TAAGACTACA GAATTTCAAT GAGCGCCAAC AGATTAAAGC CAAACTTCGT 
GGTGAAAAGA AAGAAGCTAA TCATTCTGAA GGACAAGAAG GAAGTGAAGA GGCTGACATG 
AGGCGCAAAA AAATCGAATC ACTGAAGGCC CATGCAAATG CACGTGCTGC TGTACTAAAA 
GAACAACTAG AACGAAAGAG AAAGGAGGCT TATGAGAGAG AAAAAAAAGT GTGGGAAGAG 
CATTTGGTGG CTAAAGGAGT TAAGAGTTCT GATGTTTCTC CACCTTTGGG ACAGCATGAA 
ACAGGTGGCT CTCCATCAAA GCAACAGATG AGATCTGTTA TTTCTGTAAC TTCAGCTTTG 
AAAGAAGTTG GCGTGGACAG TAGTTTAACT GATACCCGGG AAACTTCAGA AGAGATGCAA 
AAGACCAACA ATGCTATTTC AAGTAAGCGA GAAATACTTC GTAGATTAAA TGAAAATCTT 
AAAGCTCAAG AAGATGAAAA AGGAAAGCAG AATCTCTCTG ATACTTTTGA GATAAATGTT 
CATGAAGATG CCAAAGAGCA TGAAAAAGAA AAATCAGTTT CATCTGATCG CAAGAAGTGG 
GAGGCAGGAG GTCAACTTGT GATTCCTCTG GATGAGTTAA CACTAGATAC ATCCTTCTCT 
ACAACTGAAA GACATACAGT GGGAGAAGTT ATTAAATTAG GTCCTAATGG ATCTCCAAGA 
AGAGCCTGGG GGAAAAGTCC GACAGATTCT GTTCTAAAGA TACTTGGAGA AGCTGAACTA 
CAACTTCAGA CAGAACTATT AGAAAATACA ACTATTAGAA GTGAGATTTC TCCCGAAGGG 
GAAAAGTACA AACCCTTAAT TACTGGAGAA AAAAAAGTAC AATGTATTTC ACATGAAATA 
AACCCATCAG CTATTGTTGA TTCTCCTGTT GAGACAAAAA GTCCCGAGTT CAGTGAGGCA 
TCTCCACAGA TGTCATTGAA ACTGGAAGGA AATTTAGAAG AACCTGATGA TTTGGAAACA 
GAAATTCTAC AAGAGCCAAG TGGAACAAAC AAAGATGAGA GCTTGCCATG CACTATTACT 
GATGTGTGGA TTAGTGAGGA AAAAGAAACA AAGGAAACTC AGTCGGCAGA TAGGATCACC 
ATTCAGGAAA ATGAAGTTTC TGAAGATGGA GTCTCGAGTA CTGTGGACCA ACTTAGTGAC 
ATTCATATAG AGCCTGGAAC CAATGATTCT CAGCACTCTA AATGTGATGT AGATAAGTCT 
GTGCAACCGG AACCATTTTT CCATAAGGTG GTTCATTCTG AACACTTGAA CTTAGTCCCT 
CAAGTTCAAT CAGTTCAGTG TTCACCAGAA GAATCCTTTG CATTTCGATC TCACTCGCAT 
TTACCACCAA AAAATAAAAA CAAGAATTCC TTGCTGATTG GACTTTCAAC TGGTCTGTTT 
GATGCAAACA ACCCAAAGAT GTTAAGGACA TGTTCACTTC CAGATCTCTC AAAGCTGTTC 
AGAACCCTTA TGGATGTTCC CACCGTAGGA GATGTTCGTC AAGACAATCT TGAAATAGAT 
GAAATTGAAG ATGAAAACAT TAAAGAAGGA CCTTCTGATT CTGAAGACAT TGTGTTTGAA 
GAAACTGACA CAGATTTACA AGAGCTGCAG GCCTCGATGG AACAGTTACT TAGGGAACAA 
CCTGGTGAAG AATACAGTGA AGAAGAAGAG TCAGTCTTGA AGAACAGTGA TGTGGAGCCA 
ACTGCAAATG GGACAGATGT GGCAGATGAA GATGACAATC CCAGCAGTGA AAGTGCCCTG 
AACGAAGAAT GGCACTCAGA TAACAGTGAT GGTGAAATTG CTAGTGAATG TGAATGCGAT 
AGTGTCTTTA ACCATTTAGA GGAACTGAGA CTTCATCTGG AGCAGGAAAT GGGCTTTGAA 
AAATTCTTTG AGGTTTATGA GAAAATAAAG GCTATTCATG AAGATGAAGA TGAAAATATT 
GAAATTTGTT CAAAAATAGT TCAAAATATT TTGGGAAATG AACATCAGCA TCTTTATGCC 
AAGATTCTTC ATTTAGTCAT GGCAGATGGA GCCTACCAAG AAGATAATGA TGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036356.1
CDS	657..4433
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKPLQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERGILPGVRPGFPYGAAGHHHFPDADDIRKTLKRLKAVSKQANANRQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMVYLARLRQIRLQNFNERQQIKAKLRGEKKEANHSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQNLSDTFEINVHEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLGPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSEISPEGEKYKPLITGEKKVQCISHEINPSAIVDSPVETKSPEFSEASPQMSLKLEGNLEEPDDLETEILQEPSGTNKDESLPCTITDVWISEEKETKETQSADRITIQENEVSEDGVSSTVDQLSDIHIEPGTNDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGEIASECECDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	NM_012224.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012224.2

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTGGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG AGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT TCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGTTTA TCTGGCAAGA 
CTGAGGCAAA TAAGACTACA GAATTTCAAT GAGCGCCAAC AGATTAAAGC CAAACTTCGT 
GGTGAAAAGA AAGAAGCTAA TCATTCTGAA GGACAAGAAG GAAGTGAAGA GGCTGACATG 
AGGCGCAAAA AAATCGAATC ACTGAAGGCC CATGCAAATG CACGTGCTGC TGTACTAAAA 
GAACAACTAG AACGAAAGAG AAAGGAGGCT TATGAGAGAG AAAAAAAAGT GTGGGAAGAG 
CATTTGGTGG CTAAAGGAGT TAAGAGTTCT GATGTTTCTC CACCTTTGGG ACAGCATGAA 
ACAGGTGGCT CTCCATCAAA GCAACAGATG AGATCTGTTA TTTCTGTAAC TTCAGCTTTG 
AAAGAAGTTG GCGTGGACAG TAGTTTAACT GATACCCGGG AAACTTCAGA AGAGATGCAA 
AAGACCAACA ATGCTATTTC AAGTAAGCGA GAAATACTTC GTAGATTAAA TGAAAATCTT 
AAAGCTCAAG AAGATGAAAA AGGAAAGCAG AATCTCTCTG ATACTTTTGA GATAAATGTT 
CATGAAGATG CCAAAGAGCA TGAAAAAGAA AAATCAGTTT CATCTGATCG CAAGAAGTGG 
GAGGCAGGAG GTCAACTTGT GATTCCTCTG GATGAGTTAA CACTAGATAC ATCCTTCTCT 
ACAACTGAAA GACATACAGT GGGAGAAGTT ATTAAATTAG GTCCTAATGG ATCTCCAAGA 
AGAGCCTGGG GGAAAAGTCC GACAGATTCT GTTCTAAAGA TACTTGGAGA AGCTGAACTA 
CAACTTCAGA CAGAACTATT AGAAAATACA ACTATTAGAA GTGAGATTTC TCCCGAAGGG 
GAAAAGTACA AACCCTTAAT TACTGGAGAA AAAAAAGTAC AATGTATTTC ACATGAAATA 
AACCCATCAG CTATTGTTGA TTCTCCTGTT GAGACAAAAA GTCCCGAGTT CAGTGAGGCA 
TCTCCACAGA TGTCATTGAA ACTGGAAGGA AATTTAGAAG AACCTGATGA TTTGGAAACA 
GAAATTCTAC AAGAGCCAAG TGGAACAAAC AAAGATGAGA GCTTGCCATG CACTATTACT 
GATGTGTGGA TTAGTGAGGA AAAAGAAACA AAGGAAACTC AGTCGGCAGA TAGGATCACC 
ATTCAGGAAA ATGAAGTTTC TGAAGATGGA GTCTCGAGTA CTGTGGACCA ACTTAGTGAC 
ATTCATATAG AGCCTGGAAC CAATGATTCT CAGCACTCTA AATGTGATGT AGATAAGTCT 
GTGCAACCGG AACCATTTTT CCATAAGGTG GTTCATTCTG AACACTTGAA CTTAGTCCCT 
CAAGTTCAAT CAGTTCAGTG TTCACCAGAA GAATCCTTTG CATTTCGATC TCACTCGCAT 
TTACCACCAA AAAATAAAAA CAAGAATTCC TTGCTGATTG GACTTTCAAC TGGTCTGTTT 
GATGCAAACA ACCCAAAGAT GTTAAGGACA TGTTCACTTC CAGATCTCTC AAAGCTGTTC 
AGAACCCTTA TGGATGTTCC CACCGTAGGA GATGTTCGTC AAGACAATCT TGAAATAGAT 
GAAATTGAAG ATGAAAACAT TAAAGAAGGA CCTTCTGATT CTGAAGACAT TGTGTTTGAA 
GAAACTGACA CAGATTTACA AGAGCTGCAG GCCTCGATGG AACAGTTACT TAGGGAACAA 
CCTGGTGAAG AATACAGTGA AGAAGAAGAG TCAGTCTTGA AGAACAGTGA TGTGGAGCCA 
ACTGCAAATG GGACAGATGT GGCAGATGAA GATGACAATC CCAGCAGTGA AAGTGCCCTG 
AACGAAGAAT GGCACTCAGA TAACAGTGAT GGTGAAATTG CTAGTGAATG TGAATGCGAT 
AGTGTCTTTA ACCATTTAGA GGAACTGAGA CTTCATCTGG AGCAGGAAAT GGGCTTTGAA 
AAATTCTTTG AGGTTTATGA GAAAATAAAG GCTATTCATG AAGATGAAGA TGAAAATATT 
GAAATTTGTT CAAAAATAGT TCAAAATATT TTGGGAAATG AACATCAGCA TCTTTATGCC 
AAGATTCTTC ATTTAGTCAT GGCAGATGGA GCCTACCAAG AAGATAATGA TGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( NM_001374419.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001361348.1 Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu10748
Clone ID Related Accession (Same CDS sequence)	NM_012224.2 , XM_011532003.1 , NM_001374419.1 , NM_012224.3
Accession Version	NM_001374419.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC084724.6, AC116621.8 and AC116615.5. On Sep 25, 2019 this sequence version replaced XM_011532003.1. Transcript Variant: This variant (7), as well as variant 2, encodes isoform 2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.255716.1, SRR1803615.122198.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTGGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG AGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT TCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGTTTA TCTGGCAAGA 
CTGAGGCAAA TAAGACTACA GAATTTCAAT GAGCGCCAAC AGATTAAAGC CAAACTTCGT 
GGTGAAAAGA AAGAAGCTAA TCATTCTGAA GGACAAGAAG GAAGTGAAGA GGCTGACATG 
AGGCGCAAAA AAATCGAATC ACTGAAGGCC CATGCAAATG CACGTGCTGC TGTACTAAAA 
GAACAACTAG AACGAAAGAG AAAGGAGGCT TATGAGAGAG AAAAAAAAGT GTGGGAAGAG 
CATTTGGTGG CTAAAGGAGT TAAGAGTTCT GATGTTTCTC CACCTTTGGG ACAGCATGAA 
ACAGGTGGCT CTCCATCAAA GCAACAGATG AGATCTGTTA TTTCTGTAAC TTCAGCTTTG 
AAAGAAGTTG GCGTGGACAG TAGTTTAACT GATACCCGGG AAACTTCAGA AGAGATGCAA 
AAGACCAACA ATGCTATTTC AAGTAAGCGA GAAATACTTC GTAGATTAAA TGAAAATCTT 
AAAGCTCAAG AAGATGAAAA AGGAAAGCAG AATCTCTCTG ATACTTTTGA GATAAATGTT 
CATGAAGATG CCAAAGAGCA TGAAAAAGAA AAATCAGTTT CATCTGATCG CAAGAAGTGG 
GAGGCAGGAG GTCAACTTGT GATTCCTCTG GATGAGTTAA CACTAGATAC ATCCTTCTCT 
ACAACTGAAA GACATACAGT GGGAGAAGTT ATTAAATTAG GTCCTAATGG ATCTCCAAGA 
AGAGCCTGGG GGAAAAGTCC GACAGATTCT GTTCTAAAGA TACTTGGAGA AGCTGAACTA 
CAACTTCAGA CAGAACTATT AGAAAATACA ACTATTAGAA GTGAGATTTC TCCCGAAGGG 
GAAAAGTACA AACCCTTAAT TACTGGAGAA AAAAAAGTAC AATGTATTTC ACATGAAATA 
AACCCATCAG CTATTGTTGA TTCTCCTGTT GAGACAAAAA GTCCCGAGTT CAGTGAGGCA 
TCTCCACAGA TGTCATTGAA ACTGGAAGGA AATTTAGAAG AACCTGATGA TTTGGAAACA 
GAAATTCTAC AAGAGCCAAG TGGAACAAAC AAAGATGAGA GCTTGCCATG CACTATTACT 
GATGTGTGGA TTAGTGAGGA AAAAGAAACA AAGGAAACTC AGTCGGCAGA TAGGATCACC 
ATTCAGGAAA ATGAAGTTTC TGAAGATGGA GTCTCGAGTA CTGTGGACCA ACTTAGTGAC 
ATTCATATAG AGCCTGGAAC CAATGATTCT CAGCACTCTA AATGTGATGT AGATAAGTCT 
GTGCAACCGG AACCATTTTT CCATAAGGTG GTTCATTCTG AACACTTGAA CTTAGTCCCT 
CAAGTTCAAT CAGTTCAGTG TTCACCAGAA GAATCCTTTG CATTTCGATC TCACTCGCAT 
TTACCACCAA AAAATAAAAA CAAGAATTCC TTGCTGATTG GACTTTCAAC TGGTCTGTTT 
GATGCAAACA ACCCAAAGAT GTTAAGGACA TGTTCACTTC CAGATCTCTC AAAGCTGTTC 
AGAACCCTTA TGGATGTTCC CACCGTAGGA GATGTTCGTC AAGACAATCT TGAAATAGAT 
GAAATTGAAG ATGAAAACAT TAAAGAAGGA CCTTCTGATT CTGAAGACAT TGTGTTTGAA 
GAAACTGACA CAGATTTACA AGAGCTGCAG GCCTCGATGG AACAGTTACT TAGGGAACAA 
CCTGGTGAAG AATACAGTGA AGAAGAAGAG TCAGTCTTGA AGAACAGTGA TGTGGAGCCA 
ACTGCAAATG GGACAGATGT GGCAGATGAA GATGACAATC CCAGCAGTGA AAGTGCCCTG 
AACGAAGAAT GGCACTCAGA TAACAGTGAT GGTGAAATTG CTAGTGAATG TGAATGCGAT 
AGTGTCTTTA ACCATTTAGA GGAACTGAGA CTTCATCTGG AGCAGGAAAT GGGCTTTGAA 
AAATTCTTTG AGGTTTATGA GAAAATAAAG GCTATTCATG AAGATGAAGA TGAAAATATT 
GAAATTTGTT CAAAAATAGT TCAAAATATT TTGGGAAATG AACATCAGCA TCTTTATGCC 
AAGATTCTTC ATTTAGTCAT GGCAGATGGA GCCTACCAAG AAGATAATGA TGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001361348.1
CDS	535..4311
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKPLQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERGILPGVRPGFPYGAAGHHHFPDADDIRKTLKRLKAVSKQANANRQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMVYLARLRQIRLQNFNERQQIKAKLRGEKKEANHSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQNLSDTFEINVHEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLGPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSEISPEGEKYKPLITGEKKVQCISHEINPSAIVDSPVETKSPEFSEASPQMSLKLEGNLEEPDDLETEILQEPSGTNKDESLPCTITDVWISEEKETKETQSADRITIQENEVSEDGVSSTVDQLSDIHIEPGTNDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGEIASECECDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	NM_001374419.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001374419.1

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTGGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG AGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT TCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGTTTA TCTGGCAAGA 
CTGAGGCAAA TAAGACTACA GAATTTCAAT GAGCGCCAAC AGATTAAAGC CAAACTTCGT 
GGTGAAAAGA AAGAAGCTAA TCATTCTGAA GGACAAGAAG GAAGTGAAGA GGCTGACATG 
AGGCGCAAAA AAATCGAATC ACTGAAGGCC CATGCAAATG CACGTGCTGC TGTACTAAAA 
GAACAACTAG AACGAAAGAG AAAGGAGGCT TATGAGAGAG AAAAAAAAGT GTGGGAAGAG 
CATTTGGTGG CTAAAGGAGT TAAGAGTTCT GATGTTTCTC CACCTTTGGG ACAGCATGAA 
ACAGGTGGCT CTCCATCAAA GCAACAGATG AGATCTGTTA TTTCTGTAAC TTCAGCTTTG 
AAAGAAGTTG GCGTGGACAG TAGTTTAACT GATACCCGGG AAACTTCAGA AGAGATGCAA 
AAGACCAACA ATGCTATTTC AAGTAAGCGA GAAATACTTC GTAGATTAAA TGAAAATCTT 
AAAGCTCAAG AAGATGAAAA AGGAAAGCAG AATCTCTCTG ATACTTTTGA GATAAATGTT 
CATGAAGATG CCAAAGAGCA TGAAAAAGAA AAATCAGTTT CATCTGATCG CAAGAAGTGG 
GAGGCAGGAG GTCAACTTGT GATTCCTCTG GATGAGTTAA CACTAGATAC ATCCTTCTCT 
ACAACTGAAA GACATACAGT GGGAGAAGTT ATTAAATTAG GTCCTAATGG ATCTCCAAGA 
AGAGCCTGGG GGAAAAGTCC GACAGATTCT GTTCTAAAGA TACTTGGAGA AGCTGAACTA 
CAACTTCAGA CAGAACTATT AGAAAATACA ACTATTAGAA GTGAGATTTC TCCCGAAGGG 
GAAAAGTACA AACCCTTAAT TACTGGAGAA AAAAAAGTAC AATGTATTTC ACATGAAATA 
AACCCATCAG CTATTGTTGA TTCTCCTGTT GAGACAAAAA GTCCCGAGTT CAGTGAGGCA 
TCTCCACAGA TGTCATTGAA ACTGGAAGGA AATTTAGAAG AACCTGATGA TTTGGAAACA 
GAAATTCTAC AAGAGCCAAG TGGAACAAAC AAAGATGAGA GCTTGCCATG CACTATTACT 
GATGTGTGGA TTAGTGAGGA AAAAGAAACA AAGGAAACTC AGTCGGCAGA TAGGATCACC 
ATTCAGGAAA ATGAAGTTTC TGAAGATGGA GTCTCGAGTA CTGTGGACCA ACTTAGTGAC 
ATTCATATAG AGCCTGGAAC CAATGATTCT CAGCACTCTA AATGTGATGT AGATAAGTCT 
GTGCAACCGG AACCATTTTT CCATAAGGTG GTTCATTCTG AACACTTGAA CTTAGTCCCT 
CAAGTTCAAT CAGTTCAGTG TTCACCAGAA GAATCCTTTG CATTTCGATC TCACTCGCAT 
TTACCACCAA AAAATAAAAA CAAGAATTCC TTGCTGATTG GACTTTCAAC TGGTCTGTTT 
GATGCAAACA ACCCAAAGAT GTTAAGGACA TGTTCACTTC CAGATCTCTC AAAGCTGTTC 
AGAACCCTTA TGGATGTTCC CACCGTAGGA GATGTTCGTC AAGACAATCT TGAAATAGAT 
GAAATTGAAG ATGAAAACAT TAAAGAAGGA CCTTCTGATT CTGAAGACAT TGTGTTTGAA 
GAAACTGACA CAGATTTACA AGAGCTGCAG GCCTCGATGG AACAGTTACT TAGGGAACAA 
CCTGGTGAAG AATACAGTGA AGAAGAAGAG TCAGTCTTGA AGAACAGTGA TGTGGAGCCA 
ACTGCAAATG GGACAGATGT GGCAGATGAA GATGACAATC CCAGCAGTGA AAGTGCCCTG 
AACGAAGAAT GGCACTCAGA TAACAGTGAT GGTGAAATTG CTAGTGAATG TGAATGCGAT 
AGTGTCTTTA ACCATTTAGA GGAACTGAGA CTTCATCTGG AGCAGGAAAT GGGCTTTGAA 
AAATTCTTTG AGGTTTATGA GAAAATAAAG GCTATTCATG AAGATGAAGA TGAAAATATT 
GAAATTTGTT CAAAAATAGT TCAAAATATT TTGGGAAATG AACATCAGCA TCTTTATGCC 
AAGATTCTTC ATTTAGTCAT GGCAGATGGA GCCTACCAAG AAGATAATGA TGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( NM_012224.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_036356.1 Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu10748
Clone ID Related Accession (Same CDS sequence)	NM_012224.2 , XM_011532003.1 , NM_001374419.1 , NM_012224.3
Accession Version	NM_012224.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA697159.1, AB067488.1, CR933642.1, AK025658.1, AI984346.1 and AC116615.5. On Jun 2, 2019 this sequence version replaced NM_012224.2. Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Variants 2 and 7 both encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB067488.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTGGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG AGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT TCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGTTTA TCTGGCAAGA 
CTGAGGCAAA TAAGACTACA GAATTTCAAT GAGCGCCAAC AGATTAAAGC CAAACTTCGT 
GGTGAAAAGA AAGAAGCTAA TCATTCTGAA GGACAAGAAG GAAGTGAAGA GGCTGACATG 
AGGCGCAAAA AAATCGAATC ACTGAAGGCC CATGCAAATG CACGTGCTGC TGTACTAAAA 
GAACAACTAG AACGAAAGAG AAAGGAGGCT TATGAGAGAG AAAAAAAAGT GTGGGAAGAG 
CATTTGGTGG CTAAAGGAGT TAAGAGTTCT GATGTTTCTC CACCTTTGGG ACAGCATGAA 
ACAGGTGGCT CTCCATCAAA GCAACAGATG AGATCTGTTA TTTCTGTAAC TTCAGCTTTG 
AAAGAAGTTG GCGTGGACAG TAGTTTAACT GATACCCGGG AAACTTCAGA AGAGATGCAA 
AAGACCAACA ATGCTATTTC AAGTAAGCGA GAAATACTTC GTAGATTAAA TGAAAATCTT 
AAAGCTCAAG AAGATGAAAA AGGAAAGCAG AATCTCTCTG ATACTTTTGA GATAAATGTT 
CATGAAGATG CCAAAGAGCA TGAAAAAGAA AAATCAGTTT CATCTGATCG CAAGAAGTGG 
GAGGCAGGAG GTCAACTTGT GATTCCTCTG GATGAGTTAA CACTAGATAC ATCCTTCTCT 
ACAACTGAAA GACATACAGT GGGAGAAGTT ATTAAATTAG GTCCTAATGG ATCTCCAAGA 
AGAGCCTGGG GGAAAAGTCC GACAGATTCT GTTCTAAAGA TACTTGGAGA AGCTGAACTA 
CAACTTCAGA CAGAACTATT AGAAAATACA ACTATTAGAA GTGAGATTTC TCCCGAAGGG 
GAAAAGTACA AACCCTTAAT TACTGGAGAA AAAAAAGTAC AATGTATTTC ACATGAAATA 
AACCCATCAG CTATTGTTGA TTCTCCTGTT GAGACAAAAA GTCCCGAGTT CAGTGAGGCA 
TCTCCACAGA TGTCATTGAA ACTGGAAGGA AATTTAGAAG AACCTGATGA TTTGGAAACA 
GAAATTCTAC AAGAGCCAAG TGGAACAAAC AAAGATGAGA GCTTGCCATG CACTATTACT 
GATGTGTGGA TTAGTGAGGA AAAAGAAACA AAGGAAACTC AGTCGGCAGA TAGGATCACC 
ATTCAGGAAA ATGAAGTTTC TGAAGATGGA GTCTCGAGTA CTGTGGACCA ACTTAGTGAC 
ATTCATATAG AGCCTGGAAC CAATGATTCT CAGCACTCTA AATGTGATGT AGATAAGTCT 
GTGCAACCGG AACCATTTTT CCATAAGGTG GTTCATTCTG AACACTTGAA CTTAGTCCCT 
CAAGTTCAAT CAGTTCAGTG TTCACCAGAA GAATCCTTTG CATTTCGATC TCACTCGCAT 
TTACCACCAA AAAATAAAAA CAAGAATTCC TTGCTGATTG GACTTTCAAC TGGTCTGTTT 
GATGCAAACA ACCCAAAGAT GTTAAGGACA TGTTCACTTC CAGATCTCTC AAAGCTGTTC 
AGAACCCTTA TGGATGTTCC CACCGTAGGA GATGTTCGTC AAGACAATCT TGAAATAGAT 
GAAATTGAAG ATGAAAACAT TAAAGAAGGA CCTTCTGATT CTGAAGACAT TGTGTTTGAA 
GAAACTGACA CAGATTTACA AGAGCTGCAG GCCTCGATGG AACAGTTACT TAGGGAACAA 
CCTGGTGAAG AATACAGTGA AGAAGAAGAG TCAGTCTTGA AGAACAGTGA TGTGGAGCCA 
ACTGCAAATG GGACAGATGT GGCAGATGAA GATGACAATC CCAGCAGTGA AAGTGCCCTG 
AACGAAGAAT GGCACTCAGA TAACAGTGAT GGTGAAATTG CTAGTGAATG TGAATGCGAT 
AGTGTCTTTA ACCATTTAGA GGAACTGAGA CTTCATCTGG AGCAGGAAAT GGGCTTTGAA 
AAATTCTTTG AGGTTTATGA GAAAATAAAG GCTATTCATG AAGATGAAGA TGAAAATATT 
GAAATTTGTT CAAAAATAGT TCAAAATATT TTGGGAAATG AACATCAGCA TCTTTATGCC 
AAGATTCTTC ATTTAGTCAT GGCAGATGGA GCCTACCAAG AAGATAATGA TGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036356.1
CDS	613..4389
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKPLQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERGILPGVRPGFPYGAAGHHHFPDADDIRKTLKRLKAVSKQANANRQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMVYLARLRQIRLQNFNERQQIKAKLRGEKKEANHSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQNLSDTFEINVHEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLGPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSEISPEGEKYKPLITGEKKVQCISHEINPSAIVDSPVETKSPEFSEASPQMSLKLEGNLEEPDDLETEILQEPSGTNKDESLPCTITDVWISEEKETKETQSADRITIQENEVSEDGVSSTVDQLSDIHIEPGTNDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGEIASECECDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	NM_012224.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012224.3

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTGGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG AGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT TCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGTTTA TCTGGCAAGA 
CTGAGGCAAA TAAGACTACA GAATTTCAAT GAGCGCCAAC AGATTAAAGC CAAACTTCGT 
GGTGAAAAGA AAGAAGCTAA TCATTCTGAA GGACAAGAAG GAAGTGAAGA GGCTGACATG 
AGGCGCAAAA AAATCGAATC ACTGAAGGCC CATGCAAATG CACGTGCTGC TGTACTAAAA 
GAACAACTAG AACGAAAGAG AAAGGAGGCT TATGAGAGAG AAAAAAAAGT GTGGGAAGAG 
CATTTGGTGG CTAAAGGAGT TAAGAGTTCT GATGTTTCTC CACCTTTGGG ACAGCATGAA 
ACAGGTGGCT CTCCATCAAA GCAACAGATG AGATCTGTTA TTTCTGTAAC TTCAGCTTTG 
AAAGAAGTTG GCGTGGACAG TAGTTTAACT GATACCCGGG AAACTTCAGA AGAGATGCAA 
AAGACCAACA ATGCTATTTC AAGTAAGCGA GAAATACTTC GTAGATTAAA TGAAAATCTT 
AAAGCTCAAG AAGATGAAAA AGGAAAGCAG AATCTCTCTG ATACTTTTGA GATAAATGTT 
CATGAAGATG CCAAAGAGCA TGAAAAAGAA AAATCAGTTT CATCTGATCG CAAGAAGTGG 
GAGGCAGGAG GTCAACTTGT GATTCCTCTG GATGAGTTAA CACTAGATAC ATCCTTCTCT 
ACAACTGAAA GACATACAGT GGGAGAAGTT ATTAAATTAG GTCCTAATGG ATCTCCAAGA 
AGAGCCTGGG GGAAAAGTCC GACAGATTCT GTTCTAAAGA TACTTGGAGA AGCTGAACTA 
CAACTTCAGA CAGAACTATT AGAAAATACA ACTATTAGAA GTGAGATTTC TCCCGAAGGG 
GAAAAGTACA AACCCTTAAT TACTGGAGAA AAAAAAGTAC AATGTATTTC ACATGAAATA 
AACCCATCAG CTATTGTTGA TTCTCCTGTT GAGACAAAAA GTCCCGAGTT CAGTGAGGCA 
TCTCCACAGA TGTCATTGAA ACTGGAAGGA AATTTAGAAG AACCTGATGA TTTGGAAACA 
GAAATTCTAC AAGAGCCAAG TGGAACAAAC AAAGATGAGA GCTTGCCATG CACTATTACT 
GATGTGTGGA TTAGTGAGGA AAAAGAAACA AAGGAAACTC AGTCGGCAGA TAGGATCACC 
ATTCAGGAAA ATGAAGTTTC TGAAGATGGA GTCTCGAGTA CTGTGGACCA ACTTAGTGAC 
ATTCATATAG AGCCTGGAAC CAATGATTCT CAGCACTCTA AATGTGATGT AGATAAGTCT 
GTGCAACCGG AACCATTTTT CCATAAGGTG GTTCATTCTG AACACTTGAA CTTAGTCCCT 
CAAGTTCAAT CAGTTCAGTG TTCACCAGAA GAATCCTTTG CATTTCGATC TCACTCGCAT 
TTACCACCAA AAAATAAAAA CAAGAATTCC TTGCTGATTG GACTTTCAAC TGGTCTGTTT 
GATGCAAACA ACCCAAAGAT GTTAAGGACA TGTTCACTTC CAGATCTCTC AAAGCTGTTC 
AGAACCCTTA TGGATGTTCC CACCGTAGGA GATGTTCGTC AAGACAATCT TGAAATAGAT 
GAAATTGAAG ATGAAAACAT TAAAGAAGGA CCTTCTGATT CTGAAGACAT TGTGTTTGAA 
GAAACTGACA CAGATTTACA AGAGCTGCAG GCCTCGATGG AACAGTTACT TAGGGAACAA 
CCTGGTGAAG AATACAGTGA AGAAGAAGAG TCAGTCTTGA AGAACAGTGA TGTGGAGCCA 
ACTGCAAATG GGACAGATGT GGCAGATGAA GATGACAATC CCAGCAGTGA AAGTGCCCTG 
AACGAAGAAT GGCACTCAGA TAACAGTGAT GGTGAAATTG CTAGTGAATG TGAATGCGAT 
AGTGTCTTTA ACCATTTAGA GGAACTGAGA CTTCATCTGG AGCAGGAAAT GGGCTTTGAA 
AAATTCTTTG AGGTTTATGA GAAAATAAAG GCTATTCATG AAGATGAAGA TGAAAATATT 
GAAATTTGTT CAAAAATAGT TCAAAATATT TTGGGAAATG AACATCAGCA TCTTTATGCC 
AAGATTCTTC ATTTAGTCAT GGCAGATGGA GCCTACCAAG AAGATAATGA TGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( NM_001199399.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001186328.1 Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 4, mRNA.

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CloneID	OHu11534
Clone ID Related Accession (Same CDS sequence)	NM_001199399.1 , NM_001199399.2
Accession Version	NM_001199399.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek1 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA697159.1, BC114491.1, CR933642.1, AK025658.1 and AI984346.1. Transcript Variant: This variant (4) lacks three alternate in-frame exons compared to variant 1. The resulting isoform (4) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC114491.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GGCTCCTTTT 
CTGGGCAGTG GAGGGACTAT AGCTCCATCA TCTTTTTCTT CTCGAGGACA GTATGAACAT 
TACCATGCCA TTTTTGACCA AATGCAGCAA CAAAGAGCAG AAGATAATGA AGCTAAATGG 
AAAAGAGAAA TATATGGTCG AGGTCTTCCA GAAAGGCAAA AAGGGCAGCT AGCTGTAGAA 
AGAGCTAAAC AAGTAGAAGA GTTCCTGCAG CGAAAACGGG AAGCTATGCA GAATAAAGCT 
CGAGCCGAAG GACATATGGT TTATCTGGCA AGACTGAGGC AAATAAGACT ACAGAATTTC 
AATGAGCGCC AACAGATTAA AGCCAAACTT CGTGGTGAAA AGAAAGAAGC TAATCATTCT 
GAAGGACAAG AAGGAAGTGA AGAGGCTGAC ATGAGGCGCA AAAAAATCGA ATCACTGAAG 
GCCCATGCAA ATGCACGTGC TGCTGTACTA AAAGAACAAC TAGAACGAAA GAGAAAGGAG 
GCTTATGAGA GAGAAAAAAA AGTGTGGGAA GAGCATTTGG TGGCTAAAGG AGTTAAGAGT 
TCTGATGTTT CTCCACCTTT GGGACAGCAT GAAACAGGTG GCTCTCCATC AAAGCAACAG 
ATGAGATCTG TTATTTCTGT AACTTCAGCT TTGAAAGAAG TTGGCGTGGA CAGTAGTTTA 
ACTGATACCC GGGAAACTTC AGAAGAGATG CAAAAGACCA ACAATGCTAT TTCAAGTAAG 
CGAGAAATAC TTCGTAGATT AAATGAAAAT CTTAAAGCTC AAGAAGATGA AAAAGGAAAG 
CAGAATCTCT CTGATACTTT TGAGATAAAT GTTCATGAAG ATGCCAAAGA GCATGAAAAA 
GAAAAATCAG TTTCATCTGA TCGCAAGAAG TGGGAGGCAG GAGGTCAACT TGTGATTCCT 
CTGGATGAGT TAACACTAGA TACATCCTTC TCTACAACTG AAAGACATAC AGTGGGAGAA 
GTTATTAAAT TAGGTCCTAA TGGATCTCCA AGAAGAGCCT GGGGGAAAAG TCCGACAGAT 
TCTGTTCTAA AGATACTTGG AGAAGCTGAA CTACAACTTC AGACAGAACT ATTAGAAAAT 
ACAACTATTA GAAGTGAGAT TTCTCCCGAA GGGGAAAAGT ACAAACCCTT AATTACTGGA 
GAAAAAAAAG TACAATGTAT TTCACATGAA ATAAACCCAT CAGCTATTGT TGATTCTCCT 
GTTGAGACAA AAAGTCCCGA GTTCAGTGAG GCATCTCCAC AGATGTCATT GAAACTGGAA 
GGAAATTTAG AAGAACCTGA TGATTTGGAA ACAGAAATTC TACAAGAGCC AAGTGGAACA 
AACAAAGATG AGAGCTTGCC ATGCACTATT ACTGATGTGT GGATTAGTGA GGAAAAAGAA 
ACAAAGGAAA CTCAGTCGGC AGATAGGATC ACCATTCAGG AAAATGAAGT TTCTGAAGAT 
GGAGTCTCGA GTACTGTGGA CCAACTTAGT GACATTCATA TAGAGCCTGG AACCAATGAT 
TCTCAGCACT CTAAATGTGA TGTAGATAAG TCTGTGCAAC CGGAACCATT TTTCCATAAG 
GTGGTTCATT CTGAACACTT GAACTTAGTC CCTCAAGTTC AATCAGTTCA GTGTTCACCA 
GAAGAATCCT TTGCATTTCG ATCTCACTCG CATTTACCAC CAAAAAATAA AAACAAGAAT 
TCCTTGCTGA TTGGACTTTC AACTGGTCTG TTTGATGCAA ACAACCCAAA GATGTTAAGG 
ACATGTTCAC TTCCAGATCT CTCAAAGCTG TTCAGAACCC TTATGGATGT TCCCACCGTA 
GGAGATGTTC GTCAAGACAA TCTTGAAATA GATGAAATTG AAGATGAAAA CATTAAAGAA 
GGACCTTCTG ATTCTGAAGA CATTGTGTTT GAAGAAACTG ACACAGATTT ACAAGAGCTG 
CAGGCCTCGA TGGAACAGTT ACTTAGGGAA CAACCTGGTG AAGAATACAG TGAAGAAGAA 
GAGTCAGTCT TGAAGAACAG TGATGTGGAG CCAACTGCAA ATGGGACAGA TGTGGCAGAT 
GAAGATGACA ATCCCAGCAG TGAAAGTGCC CTGAACGAAG AATGGCACTC AGATAACAGT 
GATGGTGAAA TTGCTAGTGA ATGTGAATGC GATAGTGTCT TTAACCATTT AGAGGAACTG 
AGACTTCATC TGGAGCAGGA AATGGGCTTT GAAAAATTCT TTGAGGTTTA TGAGAAAATA 
AAGGCTATTC ATGAAGATGA AGATGAAAAT ATTGAAATTT GTTCAAAAAT AGTTCAAAAT 
ATTTTGGGAA ATGAACATCA GCATCTTTAT GCCAAGATTC TTCATTTAGT CATGGCAGAT 
GGAGCCTACC AAGAAGATAA TGATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186328.1
CDS	579..4148
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKPLQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMVYLARLRQIRLQNFNERQQIKAKLRGEKKEANHSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQNLSDTFEINVHEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLGPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSEISPEGEKYKPLITGEKKVQCISHEINPSAIVDSPVETKSPEFSEASPQMSLKLEGNLEEPDDLETEILQEPSGTNKDESLPCTITDVWISEEKETKETQSADRITIQENEVSEDGVSSTVDQLSDIHIEPGTNDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGEIASECECDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	NM_001199399.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199399.1

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GGCTCCTTTT 
CTGGGCAGTG GAGGGACTAT AGCTCCATCA TCTTTTTCTT CTCGAGGACA GTATGAACAT 
TACCATGCCA TTTTTGACCA AATGCAGCAA CAAAGAGCAG AAGATAATGA AGCTAAATGG 
AAAAGAGAAA TATATGGTCG AGGTCTTCCA GAAAGGCAAA AAGGGCAGCT AGCTGTAGAA 
AGAGCTAAAC AAGTAGAAGA GTTCCTGCAG CGAAAACGGG AAGCTATGCA GAATAAAGCT 
CGAGCCGAAG GACATATGGT TTATCTGGCA AGACTGAGGC AAATAAGACT ACAGAATTTC 
AATGAGCGCC AACAGATTAA AGCCAAACTT CGTGGTGAAA AGAAAGAAGC TAATCATTCT 
GAAGGACAAG AAGGAAGTGA AGAGGCTGAC ATGAGGCGCA AAAAAATCGA ATCACTGAAG 
GCCCATGCAA ATGCACGTGC TGCTGTACTA AAAGAACAAC TAGAACGAAA GAGAAAGGAG 
GCTTATGAGA GAGAAAAAAA AGTGTGGGAA GAGCATTTGG TGGCTAAAGG AGTTAAGAGT 
TCTGATGTTT CTCCACCTTT GGGACAGCAT GAAACAGGTG GCTCTCCATC AAAGCAACAG 
ATGAGATCTG TTATTTCTGT AACTTCAGCT TTGAAAGAAG TTGGCGTGGA CAGTAGTTTA 
ACTGATACCC GGGAAACTTC AGAAGAGATG CAAAAGACCA ACAATGCTAT TTCAAGTAAG 
CGAGAAATAC TTCGTAGATT AAATGAAAAT CTTAAAGCTC AAGAAGATGA AAAAGGAAAG 
CAGAATCTCT CTGATACTTT TGAGATAAAT GTTCATGAAG ATGCCAAAGA GCATGAAAAA 
GAAAAATCAG TTTCATCTGA TCGCAAGAAG TGGGAGGCAG GAGGTCAACT TGTGATTCCT 
CTGGATGAGT TAACACTAGA TACATCCTTC TCTACAACTG AAAGACATAC AGTGGGAGAA 
GTTATTAAAT TAGGTCCTAA TGGATCTCCA AGAAGAGCCT GGGGGAAAAG TCCGACAGAT 
TCTGTTCTAA AGATACTTGG AGAAGCTGAA CTACAACTTC AGACAGAACT ATTAGAAAAT 
ACAACTATTA GAAGTGAGAT TTCTCCCGAA GGGGAAAAGT ACAAACCCTT AATTACTGGA 
GAAAAAAAAG TACAATGTAT TTCACATGAA ATAAACCCAT CAGCTATTGT TGATTCTCCT 
GTTGAGACAA AAAGTCCCGA GTTCAGTGAG GCATCTCCAC AGATGTCATT GAAACTGGAA 
GGAAATTTAG AAGAACCTGA TGATTTGGAA ACAGAAATTC TACAAGAGCC AAGTGGAACA 
AACAAAGATG AGAGCTTGCC ATGCACTATT ACTGATGTGT GGATTAGTGA GGAAAAAGAA 
ACAAAGGAAA CTCAGTCGGC AGATAGGATC ACCATTCAGG AAAATGAAGT TTCTGAAGAT 
GGAGTCTCGA GTACTGTGGA CCAACTTAGT GACATTCATA TAGAGCCTGG AACCAATGAT 
TCTCAGCACT CTAAATGTGA TGTAGATAAG TCTGTGCAAC CGGAACCATT TTTCCATAAG 
GTGGTTCATT CTGAACACTT GAACTTAGTC CCTCAAGTTC AATCAGTTCA GTGTTCACCA 
GAAGAATCCT TTGCATTTCG ATCTCACTCG CATTTACCAC CAAAAAATAA AAACAAGAAT 
TCCTTGCTGA TTGGACTTTC AACTGGTCTG TTTGATGCAA ACAACCCAAA GATGTTAAGG 
ACATGTTCAC TTCCAGATCT CTCAAAGCTG TTCAGAACCC TTATGGATGT TCCCACCGTA 
GGAGATGTTC GTCAAGACAA TCTTGAAATA GATGAAATTG AAGATGAAAA CATTAAAGAA 
GGACCTTCTG ATTCTGAAGA CATTGTGTTT GAAGAAACTG ACACAGATTT ACAAGAGCTG 
CAGGCCTCGA TGGAACAGTT ACTTAGGGAA CAACCTGGTG AAGAATACAG TGAAGAAGAA 
GAGTCAGTCT TGAAGAACAG TGATGTGGAG CCAACTGCAA ATGGGACAGA TGTGGCAGAT 
GAAGATGACA ATCCCAGCAG TGAAAGTGCC CTGAACGAAG AATGGCACTC AGATAACAGT 
GATGGTGAAA TTGCTAGTGA ATGTGAATGC GATAGTGTCT TTAACCATTT AGAGGAACTG 
AGACTTCATC TGGAGCAGGA AATGGGCTTT GAAAAATTCT TTGAGGTTTA TGAGAAAATA 
AAGGCTATTC ATGAAGATGA AGATGAAAAT ATTGAAATTT GTTCAAAAAT AGTTCAAAAT 
ATTTTGGGAA ATGAACATCA GCATCTTTAT GCCAAGATTC TTCATTTAGT CATGGCAGAT 
GGAGCCTACC AAGAAGATAA TGATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( NM_001199399.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001186328.1 Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 4, mRNA.

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CloneID	OHu11534
Clone ID Related Accession (Same CDS sequence)	NM_001199399.1 , NM_001199399.2
Accession Version	NM_001199399.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek1 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA697159.1, BC114491.1, CR933642.1, AK025658.1, AI984346.1 and AC116615.5. On Jun 2, 2019 this sequence version replaced NM_001199399.1. Transcript Variant: This variant (4) lacks three alternate in-frame exons compared to variant 1. The resulting isoform (4) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC114491.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GGCTCCTTTT 
CTGGGCAGTG GAGGGACTAT AGCTCCATCA TCTTTTTCTT CTCGAGGACA GTATGAACAT 
TACCATGCCA TTTTTGACCA AATGCAGCAA CAAAGAGCAG AAGATAATGA AGCTAAATGG 
AAAAGAGAAA TATATGGTCG AGGTCTTCCA GAAAGGCAAA AAGGGCAGCT AGCTGTAGAA 
AGAGCTAAAC AAGTAGAAGA GTTCCTGCAG CGAAAACGGG AAGCTATGCA GAATAAAGCT 
CGAGCCGAAG GACATATGGT TTATCTGGCA AGACTGAGGC AAATAAGACT ACAGAATTTC 
AATGAGCGCC AACAGATTAA AGCCAAACTT CGTGGTGAAA AGAAAGAAGC TAATCATTCT 
GAAGGACAAG AAGGAAGTGA AGAGGCTGAC ATGAGGCGCA AAAAAATCGA ATCACTGAAG 
GCCCATGCAA ATGCACGTGC TGCTGTACTA AAAGAACAAC TAGAACGAAA GAGAAAGGAG 
GCTTATGAGA GAGAAAAAAA AGTGTGGGAA GAGCATTTGG TGGCTAAAGG AGTTAAGAGT 
TCTGATGTTT CTCCACCTTT GGGACAGCAT GAAACAGGTG GCTCTCCATC AAAGCAACAG 
ATGAGATCTG TTATTTCTGT AACTTCAGCT TTGAAAGAAG TTGGCGTGGA CAGTAGTTTA 
ACTGATACCC GGGAAACTTC AGAAGAGATG CAAAAGACCA ACAATGCTAT TTCAAGTAAG 
CGAGAAATAC TTCGTAGATT AAATGAAAAT CTTAAAGCTC AAGAAGATGA AAAAGGAAAG 
CAGAATCTCT CTGATACTTT TGAGATAAAT GTTCATGAAG ATGCCAAAGA GCATGAAAAA 
GAAAAATCAG TTTCATCTGA TCGCAAGAAG TGGGAGGCAG GAGGTCAACT TGTGATTCCT 
CTGGATGAGT TAACACTAGA TACATCCTTC TCTACAACTG AAAGACATAC AGTGGGAGAA 
GTTATTAAAT TAGGTCCTAA TGGATCTCCA AGAAGAGCCT GGGGGAAAAG TCCGACAGAT 
TCTGTTCTAA AGATACTTGG AGAAGCTGAA CTACAACTTC AGACAGAACT ATTAGAAAAT 
ACAACTATTA GAAGTGAGAT TTCTCCCGAA GGGGAAAAGT ACAAACCCTT AATTACTGGA 
GAAAAAAAAG TACAATGTAT TTCACATGAA ATAAACCCAT CAGCTATTGT TGATTCTCCT 
GTTGAGACAA AAAGTCCCGA GTTCAGTGAG GCATCTCCAC AGATGTCATT GAAACTGGAA 
GGAAATTTAG AAGAACCTGA TGATTTGGAA ACAGAAATTC TACAAGAGCC AAGTGGAACA 
AACAAAGATG AGAGCTTGCC ATGCACTATT ACTGATGTGT GGATTAGTGA GGAAAAAGAA 
ACAAAGGAAA CTCAGTCGGC AGATAGGATC ACCATTCAGG AAAATGAAGT TTCTGAAGAT 
GGAGTCTCGA GTACTGTGGA CCAACTTAGT GACATTCATA TAGAGCCTGG AACCAATGAT 
TCTCAGCACT CTAAATGTGA TGTAGATAAG TCTGTGCAAC CGGAACCATT TTTCCATAAG 
GTGGTTCATT CTGAACACTT GAACTTAGTC CCTCAAGTTC AATCAGTTCA GTGTTCACCA 
GAAGAATCCT TTGCATTTCG ATCTCACTCG CATTTACCAC CAAAAAATAA AAACAAGAAT 
TCCTTGCTGA TTGGACTTTC AACTGGTCTG TTTGATGCAA ACAACCCAAA GATGTTAAGG 
ACATGTTCAC TTCCAGATCT CTCAAAGCTG TTCAGAACCC TTATGGATGT TCCCACCGTA 
GGAGATGTTC GTCAAGACAA TCTTGAAATA GATGAAATTG AAGATGAAAA CATTAAAGAA 
GGACCTTCTG ATTCTGAAGA CATTGTGTTT GAAGAAACTG ACACAGATTT ACAAGAGCTG 
CAGGCCTCGA TGGAACAGTT ACTTAGGGAA CAACCTGGTG AAGAATACAG TGAAGAAGAA 
GAGTCAGTCT TGAAGAACAG TGATGTGGAG CCAACTGCAA ATGGGACAGA TGTGGCAGAT 
GAAGATGACA ATCCCAGCAG TGAAAGTGCC CTGAACGAAG AATGGCACTC AGATAACAGT 
GATGGTGAAA TTGCTAGTGA ATGTGAATGC GATAGTGTCT TTAACCATTT AGAGGAACTG 
AGACTTCATC TGGAGCAGGA AATGGGCTTT GAAAAATTCT TTGAGGTTTA TGAGAAAATA 
AAGGCTATTC ATGAAGATGA AGATGAAAAT ATTGAAATTT GTTCAAAAAT AGTTCAAAAT 
ATTTTGGGAA ATGAACATCA GCATCTTTAT GCCAAGATTC TTCATTTAGT CATGGCAGAT 
GGAGCCTACC AAGAAGATAA TGATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186328.1
CDS	535..4104
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKPLQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMVYLARLRQIRLQNFNERQQIKAKLRGEKKEANHSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQNLSDTFEINVHEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLGPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSEISPEGEKYKPLITGEKKVQCISHEINPSAIVDSPVETKSPEFSEASPQMSLKLEGNLEEPDDLETEILQEPSGTNKDESLPCTITDVWISEEKETKETQSADRITIQENEVSEDGVSSTVDQLSDIHIEPGTNDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGEIASECECDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	NM_001199399.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199399.2

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GGCTCCTTTT 
CTGGGCAGTG GAGGGACTAT AGCTCCATCA TCTTTTTCTT CTCGAGGACA GTATGAACAT 
TACCATGCCA TTTTTGACCA AATGCAGCAA CAAAGAGCAG AAGATAATGA AGCTAAATGG 
AAAAGAGAAA TATATGGTCG AGGTCTTCCA GAAAGGCAAA AAGGGCAGCT AGCTGTAGAA 
AGAGCTAAAC AAGTAGAAGA GTTCCTGCAG CGAAAACGGG AAGCTATGCA GAATAAAGCT 
CGAGCCGAAG GACATATGGT TTATCTGGCA AGACTGAGGC AAATAAGACT ACAGAATTTC 
AATGAGCGCC AACAGATTAA AGCCAAACTT CGTGGTGAAA AGAAAGAAGC TAATCATTCT 
GAAGGACAAG AAGGAAGTGA AGAGGCTGAC ATGAGGCGCA AAAAAATCGA ATCACTGAAG 
GCCCATGCAA ATGCACGTGC TGCTGTACTA AAAGAACAAC TAGAACGAAA GAGAAAGGAG 
GCTTATGAGA GAGAAAAAAA AGTGTGGGAA GAGCATTTGG TGGCTAAAGG AGTTAAGAGT 
TCTGATGTTT CTCCACCTTT GGGACAGCAT GAAACAGGTG GCTCTCCATC AAAGCAACAG 
ATGAGATCTG TTATTTCTGT AACTTCAGCT TTGAAAGAAG TTGGCGTGGA CAGTAGTTTA 
ACTGATACCC GGGAAACTTC AGAAGAGATG CAAAAGACCA ACAATGCTAT TTCAAGTAAG 
CGAGAAATAC TTCGTAGATT AAATGAAAAT CTTAAAGCTC AAGAAGATGA AAAAGGAAAG 
CAGAATCTCT CTGATACTTT TGAGATAAAT GTTCATGAAG ATGCCAAAGA GCATGAAAAA 
GAAAAATCAG TTTCATCTGA TCGCAAGAAG TGGGAGGCAG GAGGTCAACT TGTGATTCCT 
CTGGATGAGT TAACACTAGA TACATCCTTC TCTACAACTG AAAGACATAC AGTGGGAGAA 
GTTATTAAAT TAGGTCCTAA TGGATCTCCA AGAAGAGCCT GGGGGAAAAG TCCGACAGAT 
TCTGTTCTAA AGATACTTGG AGAAGCTGAA CTACAACTTC AGACAGAACT ATTAGAAAAT 
ACAACTATTA GAAGTGAGAT TTCTCCCGAA GGGGAAAAGT ACAAACCCTT AATTACTGGA 
GAAAAAAAAG TACAATGTAT TTCACATGAA ATAAACCCAT CAGCTATTGT TGATTCTCCT 
GTTGAGACAA AAAGTCCCGA GTTCAGTGAG GCATCTCCAC AGATGTCATT GAAACTGGAA 
GGAAATTTAG AAGAACCTGA TGATTTGGAA ACAGAAATTC TACAAGAGCC AAGTGGAACA 
AACAAAGATG AGAGCTTGCC ATGCACTATT ACTGATGTGT GGATTAGTGA GGAAAAAGAA 
ACAAAGGAAA CTCAGTCGGC AGATAGGATC ACCATTCAGG AAAATGAAGT TTCTGAAGAT 
GGAGTCTCGA GTACTGTGGA CCAACTTAGT GACATTCATA TAGAGCCTGG AACCAATGAT 
TCTCAGCACT CTAAATGTGA TGTAGATAAG TCTGTGCAAC CGGAACCATT TTTCCATAAG 
GTGGTTCATT CTGAACACTT GAACTTAGTC CCTCAAGTTC AATCAGTTCA GTGTTCACCA 
GAAGAATCCT TTGCATTTCG ATCTCACTCG CATTTACCAC CAAAAAATAA AAACAAGAAT 
TCCTTGCTGA TTGGACTTTC AACTGGTCTG TTTGATGCAA ACAACCCAAA GATGTTAAGG 
ACATGTTCAC TTCCAGATCT CTCAAAGCTG TTCAGAACCC TTATGGATGT TCCCACCGTA 
GGAGATGTTC GTCAAGACAA TCTTGAAATA GATGAAATTG AAGATGAAAA CATTAAAGAA 
GGACCTTCTG ATTCTGAAGA CATTGTGTTT GAAGAAACTG ACACAGATTT ACAAGAGCTG 
CAGGCCTCGA TGGAACAGTT ACTTAGGGAA CAACCTGGTG AAGAATACAG TGAAGAAGAA 
GAGTCAGTCT TGAAGAACAG TGATGTGGAG CCAACTGCAA ATGGGACAGA TGTGGCAGAT 
GAAGATGACA ATCCCAGCAG TGAAAGTGCC CTGAACGAAG AATGGCACTC AGATAACAGT 
GATGGTGAAA TTGCTAGTGA ATGTGAATGC GATAGTGTCT TTAACCATTT AGAGGAACTG 
AGACTTCATC TGGAGCAGGA AATGGGCTTT GAAAAATTCT TTGAGGTTTA TGAGAAAATA 
AAGGCTATTC ATGAAGATGA AGATGAAAAT ATTGAAATTT GTTCAAAAAT AGTTCAAAAT 
ATTTTGGGAA ATGAACATCA GCATCTTTAT GCCAAGATTC TTCATTTAGT CATGGCAGAT 
GGAGCCTACC AAGAAGATAA TGATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( NM_001199397.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001186326.1 Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21522
Clone ID Related Accession (Same CDS sequence)	NM_001199397.3 , NM_001374418.1 , NM_001199397.1 , NM_001199397.2
Accession Version	NM_001199397.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC084724.6, AC116621.8 and AC116615.5. On Sep 25, 2019 this sequence version replaced NM_001199397.2. Transcript Variant: This variant (1) encodes the longest isoform (1). Variants 1 and 6 both encode the same isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CR933642.1, SRR1803617.111804.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTGGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG AGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT TCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGGAAT CCTGCAAAAC 
CTGGCAGCTA TGTATGGAGG CAGGCCCAGC TCTTCAAGAG GAGGGAAGCC AAGAAACAAA 
GAGGAAGAGG TTTATCTGGC AAGACTGAGG CAAATAAGAC TACAGAATTT CAATGAGCGC 
CAACAGATTA AAGCCAAACT TCGTGGTGAA AAGAAAGAAG CTAATCATTC TGAAGGACAA 
GAAGGAAGTG AAGAGGCTGA CATGAGGCGC AAAAAAATCG AATCACTGAA GGCCCATGCA 
AATGCACGTG CTGCTGTACT AAAAGAACAA CTAGAACGAA AGAGAAAGGA GGCTTATGAG 
AGAGAAAAAA AAGTGTGGGA AGAGCATTTG GTGGCTAAAG GAGTTAAGAG TTCTGATGTT 
TCTCCACCTT TGGGACAGCA TGAAACAGGT GGCTCTCCAT CAAAGCAACA GATGAGATCT 
GTTATTTCTG TAACTTCAGC TTTGAAAGAA GTTGGCGTGG ACAGTAGTTT AACTGATACC 
CGGGAAACTT CAGAAGAGAT GCAAAAGACC AACAATGCTA TTTCAAGTAA GCGAGAAATA 
CTTCGTAGAT TAAATGAAAA TCTTAAAGCT CAAGAAGATG AAAAAGGAAA GCAGAATCTC 
TCTGATACTT TTGAGATAAA TGTTCATGAA GATGCCAAAG AGCATGAAAA AGAAAAATCA 
GTTTCATCTG ATCGCAAGAA GTGGGAGGCA GGAGGTCAAC TTGTGATTCC TCTGGATGAG 
TTAACACTAG ATACATCCTT CTCTACAACT GAAAGACATA CAGTGGGAGA AGTTATTAAA 
TTAGGTCCTA ATGGATCTCC AAGAAGAGCC TGGGGGAAAA GTCCGACAGA TTCTGTTCTA 
AAGATACTTG GAGAAGCTGA ACTACAACTT CAGACAGAAC TATTAGAAAA TACAACTATT 
AGAAGTGAGA TTTCTCCCGA AGGGGAAAAG TACAAACCCT TAATTACTGG AGAAAAAAAA 
GTACAATGTA TTTCACATGA AATAAACCCA TCAGCTATTG TTGATTCTCC TGTTGAGACA 
AAAAGTCCCG AGTTCAGTGA GGCATCTCCA CAGATGTCAT TGAAACTGGA AGGAAATTTA 
GAAGAACCTG ATGATTTGGA AACAGAAATT CTACAAGAGC CAAGTGGAAC AAACAAAGAT 
GAGAGCTTGC CATGCACTAT TACTGATGTG TGGATTAGTG AGGAAAAAGA AACAAAGGAA 
ACTCAGTCGG CAGATAGGAT CACCATTCAG GAAAATGAAG TTTCTGAAGA TGGAGTCTCG 
AGTACTGTGG ACCAACTTAG TGACATTCAT ATAGAGCCTG GAACCAATGA TTCTCAGCAC 
TCTAAATGTG ATGTAGATAA GTCTGTGCAA CCGGAACCAT TTTTCCATAA GGTGGTTCAT 
TCTGAACACT TGAACTTAGT CCCTCAAGTT CAATCAGTTC AGTGTTCACC AGAAGAATCC 
TTTGCATTTC GATCTCACTC GCATTTACCA CCAAAAAATA AAAACAAGAA TTCCTTGCTG 
ATTGGACTTT CAACTGGTCT GTTTGATGCA AACAACCCAA AGATGTTAAG GACATGTTCA 
CTTCCAGATC TCTCAAAGCT GTTCAGAACC CTTATGGATG TTCCCACCGT AGGAGATGTT 
CGTCAAGACA ATCTTGAAAT AGATGAAATT GAAGATGAAA ACATTAAAGA AGGACCTTCT 
GATTCTGAAG ACATTGTGTT TGAAGAAACT GACACAGATT TACAAGAGCT GCAGGCCTCG 
ATGGAACAGT TACTTAGGGA ACAACCTGGT GAAGAATACA GTGAAGAAGA AGAGTCAGTC 
TTGAAGAACA GTGATGTGGA GCCAACTGCA AATGGGACAG ATGTGGCAGA TGAAGATGAC 
AATCCCAGCA GTGAAAGTGC CCTGAACGAA GAATGGCACT CAGATAACAG TGATGGTGAA 
ATTGCTAGTG AATGTGAATG CGATAGTGTC TTTAACCATT TAGAGGAACT GAGACTTCAT 
CTGGAGCAGG AAATGGGCTT TGAAAAATTC TTTGAGGTTT ATGAGAAAAT AAAGGCTATT 
CATGAAGATG AAGATGAAAA TATTGAAATT TGTTCAAAAA TAGTTCAAAA TATTTTGGGA 
AATGAACATC AGCATCTTTA TGCCAAGATT CTTCATTTAG TCATGGCAGA TGGAGCCTAC 
CAAGAAGATA ATGATGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186326.1
CDS	535..4395
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKPLQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERGILPGVRPGFPYGAAGHHHFPDADDIRKTLKRLKAVSKQANANRQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMGILQNLAAMYGGRPSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIKAKLRGEKKEANHSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQNLSDTFEINVHEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLGPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSEISPEGEKYKPLITGEKKVQCISHEINPSAIVDSPVETKSPEFSEASPQMSLKLEGNLEEPDDLETEILQEPSGTNKDESLPCTITDVWISEEKETKETQSADRITIQENEVSEDGVSSTVDQLSDIHIEPGTNDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGEIASECECDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	NM_001199397.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199397.3

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTGGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG AGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT TCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGGAAT CCTGCAAAAC 
CTGGCAGCTA TGTATGGAGG CAGGCCCAGC TCTTCAAGAG GAGGGAAGCC AAGAAACAAA 
GAGGAAGAGG TTTATCTGGC AAGACTGAGG CAAATAAGAC TACAGAATTT CAATGAGCGC 
CAACAGATTA AAGCCAAACT TCGTGGTGAA AAGAAAGAAG CTAATCATTC TGAAGGACAA 
GAAGGAAGTG AAGAGGCTGA CATGAGGCGC AAAAAAATCG AATCACTGAA GGCCCATGCA 
AATGCACGTG CTGCTGTACT AAAAGAACAA CTAGAACGAA AGAGAAAGGA GGCTTATGAG 
AGAGAAAAAA AAGTGTGGGA AGAGCATTTG GTGGCTAAAG GAGTTAAGAG TTCTGATGTT 
TCTCCACCTT TGGGACAGCA TGAAACAGGT GGCTCTCCAT CAAAGCAACA GATGAGATCT 
GTTATTTCTG TAACTTCAGC TTTGAAAGAA GTTGGCGTGG ACAGTAGTTT AACTGATACC 
CGGGAAACTT CAGAAGAGAT GCAAAAGACC AACAATGCTA TTTCAAGTAA GCGAGAAATA 
CTTCGTAGAT TAAATGAAAA TCTTAAAGCT CAAGAAGATG AAAAAGGAAA GCAGAATCTC 
TCTGATACTT TTGAGATAAA TGTTCATGAA GATGCCAAAG AGCATGAAAA AGAAAAATCA 
GTTTCATCTG ATCGCAAGAA GTGGGAGGCA GGAGGTCAAC TTGTGATTCC TCTGGATGAG 
TTAACACTAG ATACATCCTT CTCTACAACT GAAAGACATA CAGTGGGAGA AGTTATTAAA 
TTAGGTCCTA ATGGATCTCC AAGAAGAGCC TGGGGGAAAA GTCCGACAGA TTCTGTTCTA 
AAGATACTTG GAGAAGCTGA ACTACAACTT CAGACAGAAC TATTAGAAAA TACAACTATT 
AGAAGTGAGA TTTCTCCCGA AGGGGAAAAG TACAAACCCT TAATTACTGG AGAAAAAAAA 
GTACAATGTA TTTCACATGA AATAAACCCA TCAGCTATTG TTGATTCTCC TGTTGAGACA 
AAAAGTCCCG AGTTCAGTGA GGCATCTCCA CAGATGTCAT TGAAACTGGA AGGAAATTTA 
GAAGAACCTG ATGATTTGGA AACAGAAATT CTACAAGAGC CAAGTGGAAC AAACAAAGAT 
GAGAGCTTGC CATGCACTAT TACTGATGTG TGGATTAGTG AGGAAAAAGA AACAAAGGAA 
ACTCAGTCGG CAGATAGGAT CACCATTCAG GAAAATGAAG TTTCTGAAGA TGGAGTCTCG 
AGTACTGTGG ACCAACTTAG TGACATTCAT ATAGAGCCTG GAACCAATGA TTCTCAGCAC 
TCTAAATGTG ATGTAGATAA GTCTGTGCAA CCGGAACCAT TTTTCCATAA GGTGGTTCAT 
TCTGAACACT TGAACTTAGT CCCTCAAGTT CAATCAGTTC AGTGTTCACC AGAAGAATCC 
TTTGCATTTC GATCTCACTC GCATTTACCA CCAAAAAATA AAAACAAGAA TTCCTTGCTG 
ATTGGACTTT CAACTGGTCT GTTTGATGCA AACAACCCAA AGATGTTAAG GACATGTTCA 
CTTCCAGATC TCTCAAAGCT GTTCAGAACC CTTATGGATG TTCCCACCGT AGGAGATGTT 
CGTCAAGACA ATCTTGAAAT AGATGAAATT GAAGATGAAA ACATTAAAGA AGGACCTTCT 
GATTCTGAAG ACATTGTGTT TGAAGAAACT GACACAGATT TACAAGAGCT GCAGGCCTCG 
ATGGAACAGT TACTTAGGGA ACAACCTGGT GAAGAATACA GTGAAGAAGA AGAGTCAGTC 
TTGAAGAACA GTGATGTGGA GCCAACTGCA AATGGGACAG ATGTGGCAGA TGAAGATGAC 
AATCCCAGCA GTGAAAGTGC CCTGAACGAA GAATGGCACT CAGATAACAG TGATGGTGAA 
ATTGCTAGTG AATGTGAATG CGATAGTGTC TTTAACCATT TAGAGGAACT GAGACTTCAT 
CTGGAGCAGG AAATGGGCTT TGAAAAATTC TTTGAGGTTT ATGAGAAAAT AAAGGCTATT 
CATGAAGATG AAGATGAAAA TATTGAAATT TGTTCAAAAA TAGTTCAAAA TATTTTGGGA 
AATGAACATC AGCATCTTTA TGCCAAGATT CTTCATTTAG TCATGGCAGA TGGAGCCTAC 
CAAGAAGATA ATGATGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( NM_001374418.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001361347.1 Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 6, mRNA.

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CloneID	OHu21522
Clone ID Related Accession (Same CDS sequence)	NM_001199397.3 , NM_001374418.1 , NM_001199397.1 , NM_001199397.2
Accession Version	NM_001374418.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC084724.6, AC116621.8 and AC116615.5. Transcript Variant: This variant (6), as well as variant 1, encodes isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.23437.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTGGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG AGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT TCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGGAAT CCTGCAAAAC 
CTGGCAGCTA TGTATGGAGG CAGGCCCAGC TCTTCAAGAG GAGGGAAGCC AAGAAACAAA 
GAGGAAGAGG TTTATCTGGC AAGACTGAGG CAAATAAGAC TACAGAATTT CAATGAGCGC 
CAACAGATTA AAGCCAAACT TCGTGGTGAA AAGAAAGAAG CTAATCATTC TGAAGGACAA 
GAAGGAAGTG AAGAGGCTGA CATGAGGCGC AAAAAAATCG AATCACTGAA GGCCCATGCA 
AATGCACGTG CTGCTGTACT AAAAGAACAA CTAGAACGAA AGAGAAAGGA GGCTTATGAG 
AGAGAAAAAA AAGTGTGGGA AGAGCATTTG GTGGCTAAAG GAGTTAAGAG TTCTGATGTT 
TCTCCACCTT TGGGACAGCA TGAAACAGGT GGCTCTCCAT CAAAGCAACA GATGAGATCT 
GTTATTTCTG TAACTTCAGC TTTGAAAGAA GTTGGCGTGG ACAGTAGTTT AACTGATACC 
CGGGAAACTT CAGAAGAGAT GCAAAAGACC AACAATGCTA TTTCAAGTAA GCGAGAAATA 
CTTCGTAGAT TAAATGAAAA TCTTAAAGCT CAAGAAGATG AAAAAGGAAA GCAGAATCTC 
TCTGATACTT TTGAGATAAA TGTTCATGAA GATGCCAAAG AGCATGAAAA AGAAAAATCA 
GTTTCATCTG ATCGCAAGAA GTGGGAGGCA GGAGGTCAAC TTGTGATTCC TCTGGATGAG 
TTAACACTAG ATACATCCTT CTCTACAACT GAAAGACATA CAGTGGGAGA AGTTATTAAA 
TTAGGTCCTA ATGGATCTCC AAGAAGAGCC TGGGGGAAAA GTCCGACAGA TTCTGTTCTA 
AAGATACTTG GAGAAGCTGA ACTACAACTT CAGACAGAAC TATTAGAAAA TACAACTATT 
AGAAGTGAGA TTTCTCCCGA AGGGGAAAAG TACAAACCCT TAATTACTGG AGAAAAAAAA 
GTACAATGTA TTTCACATGA AATAAACCCA TCAGCTATTG TTGATTCTCC TGTTGAGACA 
AAAAGTCCCG AGTTCAGTGA GGCATCTCCA CAGATGTCAT TGAAACTGGA AGGAAATTTA 
GAAGAACCTG ATGATTTGGA AACAGAAATT CTACAAGAGC CAAGTGGAAC AAACAAAGAT 
GAGAGCTTGC CATGCACTAT TACTGATGTG TGGATTAGTG AGGAAAAAGA AACAAAGGAA 
ACTCAGTCGG CAGATAGGAT CACCATTCAG GAAAATGAAG TTTCTGAAGA TGGAGTCTCG 
AGTACTGTGG ACCAACTTAG TGACATTCAT ATAGAGCCTG GAACCAATGA TTCTCAGCAC 
TCTAAATGTG ATGTAGATAA GTCTGTGCAA CCGGAACCAT TTTTCCATAA GGTGGTTCAT 
TCTGAACACT TGAACTTAGT CCCTCAAGTT CAATCAGTTC AGTGTTCACC AGAAGAATCC 
TTTGCATTTC GATCTCACTC GCATTTACCA CCAAAAAATA AAAACAAGAA TTCCTTGCTG 
ATTGGACTTT CAACTGGTCT GTTTGATGCA AACAACCCAA AGATGTTAAG GACATGTTCA 
CTTCCAGATC TCTCAAAGCT GTTCAGAACC CTTATGGATG TTCCCACCGT AGGAGATGTT 
CGTCAAGACA ATCTTGAAAT AGATGAAATT GAAGATGAAA ACATTAAAGA AGGACCTTCT 
GATTCTGAAG ACATTGTGTT TGAAGAAACT GACACAGATT TACAAGAGCT GCAGGCCTCG 
ATGGAACAGT TACTTAGGGA ACAACCTGGT GAAGAATACA GTGAAGAAGA AGAGTCAGTC 
TTGAAGAACA GTGATGTGGA GCCAACTGCA AATGGGACAG ATGTGGCAGA TGAAGATGAC 
AATCCCAGCA GTGAAAGTGC CCTGAACGAA GAATGGCACT CAGATAACAG TGATGGTGAA 
ATTGCTAGTG AATGTGAATG CGATAGTGTC TTTAACCATT TAGAGGAACT GAGACTTCAT 
CTGGAGCAGG AAATGGGCTT TGAAAAATTC TTTGAGGTTT ATGAGAAAAT AAAGGCTATT 
CATGAAGATG AAGATGAAAA TATTGAAATT TGTTCAAAAA TAGTTCAAAA TATTTTGGGA 
AATGAACATC AGCATCTTTA TGCCAAGATT CTTCATTTAG TCATGGCAGA TGGAGCCTAC 
CAAGAAGATA ATGATGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001361347.1
CDS	613..4473
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKPLQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERGILPGVRPGFPYGAAGHHHFPDADDIRKTLKRLKAVSKQANANRQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMGILQNLAAMYGGRPSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIKAKLRGEKKEANHSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQNLSDTFEINVHEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLGPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSEISPEGEKYKPLITGEKKVQCISHEINPSAIVDSPVETKSPEFSEASPQMSLKLEGNLEEPDDLETEILQEPSGTNKDESLPCTITDVWISEEKETKETQSADRITIQENEVSEDGVSSTVDQLSDIHIEPGTNDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGEIASECECDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	NM_001374418.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001374418.1

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTGGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG AGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT TCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGGAAT CCTGCAAAAC 
CTGGCAGCTA TGTATGGAGG CAGGCCCAGC TCTTCAAGAG GAGGGAAGCC AAGAAACAAA 
GAGGAAGAGG TTTATCTGGC AAGACTGAGG CAAATAAGAC TACAGAATTT CAATGAGCGC 
CAACAGATTA AAGCCAAACT TCGTGGTGAA AAGAAAGAAG CTAATCATTC TGAAGGACAA 
GAAGGAAGTG AAGAGGCTGA CATGAGGCGC AAAAAAATCG AATCACTGAA GGCCCATGCA 
AATGCACGTG CTGCTGTACT AAAAGAACAA CTAGAACGAA AGAGAAAGGA GGCTTATGAG 
AGAGAAAAAA AAGTGTGGGA AGAGCATTTG GTGGCTAAAG GAGTTAAGAG TTCTGATGTT 
TCTCCACCTT TGGGACAGCA TGAAACAGGT GGCTCTCCAT CAAAGCAACA GATGAGATCT 
GTTATTTCTG TAACTTCAGC TTTGAAAGAA GTTGGCGTGG ACAGTAGTTT AACTGATACC 
CGGGAAACTT CAGAAGAGAT GCAAAAGACC AACAATGCTA TTTCAAGTAA GCGAGAAATA 
CTTCGTAGAT TAAATGAAAA TCTTAAAGCT CAAGAAGATG AAAAAGGAAA GCAGAATCTC 
TCTGATACTT TTGAGATAAA TGTTCATGAA GATGCCAAAG AGCATGAAAA AGAAAAATCA 
GTTTCATCTG ATCGCAAGAA GTGGGAGGCA GGAGGTCAAC TTGTGATTCC TCTGGATGAG 
TTAACACTAG ATACATCCTT CTCTACAACT GAAAGACATA CAGTGGGAGA AGTTATTAAA 
TTAGGTCCTA ATGGATCTCC AAGAAGAGCC TGGGGGAAAA GTCCGACAGA TTCTGTTCTA 
AAGATACTTG GAGAAGCTGA ACTACAACTT CAGACAGAAC TATTAGAAAA TACAACTATT 
AGAAGTGAGA TTTCTCCCGA AGGGGAAAAG TACAAACCCT TAATTACTGG AGAAAAAAAA 
GTACAATGTA TTTCACATGA AATAAACCCA TCAGCTATTG TTGATTCTCC TGTTGAGACA 
AAAAGTCCCG AGTTCAGTGA GGCATCTCCA CAGATGTCAT TGAAACTGGA AGGAAATTTA 
GAAGAACCTG ATGATTTGGA AACAGAAATT CTACAAGAGC CAAGTGGAAC AAACAAAGAT 
GAGAGCTTGC CATGCACTAT TACTGATGTG TGGATTAGTG AGGAAAAAGA AACAAAGGAA 
ACTCAGTCGG CAGATAGGAT CACCATTCAG GAAAATGAAG TTTCTGAAGA TGGAGTCTCG 
AGTACTGTGG ACCAACTTAG TGACATTCAT ATAGAGCCTG GAACCAATGA TTCTCAGCAC 
TCTAAATGTG ATGTAGATAA GTCTGTGCAA CCGGAACCAT TTTTCCATAA GGTGGTTCAT 
TCTGAACACT TGAACTTAGT CCCTCAAGTT CAATCAGTTC AGTGTTCACC AGAAGAATCC 
TTTGCATTTC GATCTCACTC GCATTTACCA CCAAAAAATA AAAACAAGAA TTCCTTGCTG 
ATTGGACTTT CAACTGGTCT GTTTGATGCA AACAACCCAA AGATGTTAAG GACATGTTCA 
CTTCCAGATC TCTCAAAGCT GTTCAGAACC CTTATGGATG TTCCCACCGT AGGAGATGTT 
CGTCAAGACA ATCTTGAAAT AGATGAAATT GAAGATGAAA ACATTAAAGA AGGACCTTCT 
GATTCTGAAG ACATTGTGTT TGAAGAAACT GACACAGATT TACAAGAGCT GCAGGCCTCG 
ATGGAACAGT TACTTAGGGA ACAACCTGGT GAAGAATACA GTGAAGAAGA AGAGTCAGTC 
TTGAAGAACA GTGATGTGGA GCCAACTGCA AATGGGACAG ATGTGGCAGA TGAAGATGAC 
AATCCCAGCA GTGAAAGTGC CCTGAACGAA GAATGGCACT CAGATAACAG TGATGGTGAA 
ATTGCTAGTG AATGTGAATG CGATAGTGTC TTTAACCATT TAGAGGAACT GAGACTTCAT 
CTGGAGCAGG AAATGGGCTT TGAAAAATTC TTTGAGGTTT ATGAGAAAAT AAAGGCTATT 
CATGAAGATG AAGATGAAAA TATTGAAATT TGTTCAAAAA TAGTTCAAAA TATTTTGGGA 
AATGAACATC AGCATCTTTA TGCCAAGATT CTTCATTTAG TCATGGCAGA TGGAGCCTAC 
CAAGAAGATA ATGATGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( NM_001199397.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001186326.1 Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21522
Clone ID Related Accession (Same CDS sequence)	NM_001199397.3 , NM_001374418.1 , NM_001199397.1 , NM_001199397.2
Accession Version	NM_001199397.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA697159.1, AC084724.6, AB067488.1, AC116621.8, AC116615.5, AK025658.1 and AI984346.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.111804.1, CR933642.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTGGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG AGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT TCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGGAAT CCTGCAAAAC 
CTGGCAGCTA TGTATGGAGG CAGGCCCAGC TCTTCAAGAG GAGGGAAGCC AAGAAACAAA 
GAGGAAGAGG TTTATCTGGC AAGACTGAGG CAAATAAGAC TACAGAATTT CAATGAGCGC 
CAACAGATTA AAGCCAAACT TCGTGGTGAA AAGAAAGAAG CTAATCATTC TGAAGGACAA 
GAAGGAAGTG AAGAGGCTGA CATGAGGCGC AAAAAAATCG AATCACTGAA GGCCCATGCA 
AATGCACGTG CTGCTGTACT AAAAGAACAA CTAGAACGAA AGAGAAAGGA GGCTTATGAG 
AGAGAAAAAA AAGTGTGGGA AGAGCATTTG GTGGCTAAAG GAGTTAAGAG TTCTGATGTT 
TCTCCACCTT TGGGACAGCA TGAAACAGGT GGCTCTCCAT CAAAGCAACA GATGAGATCT 
GTTATTTCTG TAACTTCAGC TTTGAAAGAA GTTGGCGTGG ACAGTAGTTT AACTGATACC 
CGGGAAACTT CAGAAGAGAT GCAAAAGACC AACAATGCTA TTTCAAGTAA GCGAGAAATA 
CTTCGTAGAT TAAATGAAAA TCTTAAAGCT CAAGAAGATG AAAAAGGAAA GCAGAATCTC 
TCTGATACTT TTGAGATAAA TGTTCATGAA GATGCCAAAG AGCATGAAAA AGAAAAATCA 
GTTTCATCTG ATCGCAAGAA GTGGGAGGCA GGAGGTCAAC TTGTGATTCC TCTGGATGAG 
TTAACACTAG ATACATCCTT CTCTACAACT GAAAGACATA CAGTGGGAGA AGTTATTAAA 
TTAGGTCCTA ATGGATCTCC AAGAAGAGCC TGGGGGAAAA GTCCGACAGA TTCTGTTCTA 
AAGATACTTG GAGAAGCTGA ACTACAACTT CAGACAGAAC TATTAGAAAA TACAACTATT 
AGAAGTGAGA TTTCTCCCGA AGGGGAAAAG TACAAACCCT TAATTACTGG AGAAAAAAAA 
GTACAATGTA TTTCACATGA AATAAACCCA TCAGCTATTG TTGATTCTCC TGTTGAGACA 
AAAAGTCCCG AGTTCAGTGA GGCATCTCCA CAGATGTCAT TGAAACTGGA AGGAAATTTA 
GAAGAACCTG ATGATTTGGA AACAGAAATT CTACAAGAGC CAAGTGGAAC AAACAAAGAT 
GAGAGCTTGC CATGCACTAT TACTGATGTG TGGATTAGTG AGGAAAAAGA AACAAAGGAA 
ACTCAGTCGG CAGATAGGAT CACCATTCAG GAAAATGAAG TTTCTGAAGA TGGAGTCTCG 
AGTACTGTGG ACCAACTTAG TGACATTCAT ATAGAGCCTG GAACCAATGA TTCTCAGCAC 
TCTAAATGTG ATGTAGATAA GTCTGTGCAA CCGGAACCAT TTTTCCATAA GGTGGTTCAT 
TCTGAACACT TGAACTTAGT CCCTCAAGTT CAATCAGTTC AGTGTTCACC AGAAGAATCC 
TTTGCATTTC GATCTCACTC GCATTTACCA CCAAAAAATA AAAACAAGAA TTCCTTGCTG 
ATTGGACTTT CAACTGGTCT GTTTGATGCA AACAACCCAA AGATGTTAAG GACATGTTCA 
CTTCCAGATC TCTCAAAGCT GTTCAGAACC CTTATGGATG TTCCCACCGT AGGAGATGTT 
CGTCAAGACA ATCTTGAAAT AGATGAAATT GAAGATGAAA ACATTAAAGA AGGACCTTCT 
GATTCTGAAG ACATTGTGTT TGAAGAAACT GACACAGATT TACAAGAGCT GCAGGCCTCG 
ATGGAACAGT TACTTAGGGA ACAACCTGGT GAAGAATACA GTGAAGAAGA AGAGTCAGTC 
TTGAAGAACA GTGATGTGGA GCCAACTGCA AATGGGACAG ATGTGGCAGA TGAAGATGAC 
AATCCCAGCA GTGAAAGTGC CCTGAACGAA GAATGGCACT CAGATAACAG TGATGGTGAA 
ATTGCTAGTG AATGTGAATG CGATAGTGTC TTTAACCATT TAGAGGAACT GAGACTTCAT 
CTGGAGCAGG AAATGGGCTT TGAAAAATTC TTTGAGGTTT ATGAGAAAAT AAAGGCTATT 
CATGAAGATG AAGATGAAAA TATTGAAATT TGTTCAAAAA TAGTTCAAAA TATTTTGGGA 
AATGAACATC AGCATCTTTA TGCCAAGATT CTTCATTTAG TCATGGCAGA TGGAGCCTAC 
CAAGAAGATA ATGATGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186326.1
CDS	579..4439
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKPLQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERGILPGVRPGFPYGAAGHHHFPDADDIRKTLKRLKAVSKQANANRQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMGILQNLAAMYGGRPSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIKAKLRGEKKEANHSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQNLSDTFEINVHEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLGPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSEISPEGEKYKPLITGEKKVQCISHEINPSAIVDSPVETKSPEFSEASPQMSLKLEGNLEEPDDLETEILQEPSGTNKDESLPCTITDVWISEEKETKETQSADRITIQENEVSEDGVSSTVDQLSDIHIEPGTNDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGEIASECECDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	NM_001199397.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199397.1

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTGGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG AGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT TCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGGAAT CCTGCAAAAC 
CTGGCAGCTA TGTATGGAGG CAGGCCCAGC TCTTCAAGAG GAGGGAAGCC AAGAAACAAA 
GAGGAAGAGG TTTATCTGGC AAGACTGAGG CAAATAAGAC TACAGAATTT CAATGAGCGC 
CAACAGATTA AAGCCAAACT TCGTGGTGAA AAGAAAGAAG CTAATCATTC TGAAGGACAA 
GAAGGAAGTG AAGAGGCTGA CATGAGGCGC AAAAAAATCG AATCACTGAA GGCCCATGCA 
AATGCACGTG CTGCTGTACT AAAAGAACAA CTAGAACGAA AGAGAAAGGA GGCTTATGAG 
AGAGAAAAAA AAGTGTGGGA AGAGCATTTG GTGGCTAAAG GAGTTAAGAG TTCTGATGTT 
TCTCCACCTT TGGGACAGCA TGAAACAGGT GGCTCTCCAT CAAAGCAACA GATGAGATCT 
GTTATTTCTG TAACTTCAGC TTTGAAAGAA GTTGGCGTGG ACAGTAGTTT AACTGATACC 
CGGGAAACTT CAGAAGAGAT GCAAAAGACC AACAATGCTA TTTCAAGTAA GCGAGAAATA 
CTTCGTAGAT TAAATGAAAA TCTTAAAGCT CAAGAAGATG AAAAAGGAAA GCAGAATCTC 
TCTGATACTT TTGAGATAAA TGTTCATGAA GATGCCAAAG AGCATGAAAA AGAAAAATCA 
GTTTCATCTG ATCGCAAGAA GTGGGAGGCA GGAGGTCAAC TTGTGATTCC TCTGGATGAG 
TTAACACTAG ATACATCCTT CTCTACAACT GAAAGACATA CAGTGGGAGA AGTTATTAAA 
TTAGGTCCTA ATGGATCTCC AAGAAGAGCC TGGGGGAAAA GTCCGACAGA TTCTGTTCTA 
AAGATACTTG GAGAAGCTGA ACTACAACTT CAGACAGAAC TATTAGAAAA TACAACTATT 
AGAAGTGAGA TTTCTCCCGA AGGGGAAAAG TACAAACCCT TAATTACTGG AGAAAAAAAA 
GTACAATGTA TTTCACATGA AATAAACCCA TCAGCTATTG TTGATTCTCC TGTTGAGACA 
AAAAGTCCCG AGTTCAGTGA GGCATCTCCA CAGATGTCAT TGAAACTGGA AGGAAATTTA 
GAAGAACCTG ATGATTTGGA AACAGAAATT CTACAAGAGC CAAGTGGAAC AAACAAAGAT 
GAGAGCTTGC CATGCACTAT TACTGATGTG TGGATTAGTG AGGAAAAAGA AACAAAGGAA 
ACTCAGTCGG CAGATAGGAT CACCATTCAG GAAAATGAAG TTTCTGAAGA TGGAGTCTCG 
AGTACTGTGG ACCAACTTAG TGACATTCAT ATAGAGCCTG GAACCAATGA TTCTCAGCAC 
TCTAAATGTG ATGTAGATAA GTCTGTGCAA CCGGAACCAT TTTTCCATAA GGTGGTTCAT 
TCTGAACACT TGAACTTAGT CCCTCAAGTT CAATCAGTTC AGTGTTCACC AGAAGAATCC 
TTTGCATTTC GATCTCACTC GCATTTACCA CCAAAAAATA AAAACAAGAA TTCCTTGCTG 
ATTGGACTTT CAACTGGTCT GTTTGATGCA AACAACCCAA AGATGTTAAG GACATGTTCA 
CTTCCAGATC TCTCAAAGCT GTTCAGAACC CTTATGGATG TTCCCACCGT AGGAGATGTT 
CGTCAAGACA ATCTTGAAAT AGATGAAATT GAAGATGAAA ACATTAAAGA AGGACCTTCT 
GATTCTGAAG ACATTGTGTT TGAAGAAACT GACACAGATT TACAAGAGCT GCAGGCCTCG 
ATGGAACAGT TACTTAGGGA ACAACCTGGT GAAGAATACA GTGAAGAAGA AGAGTCAGTC 
TTGAAGAACA GTGATGTGGA GCCAACTGCA AATGGGACAG ATGTGGCAGA TGAAGATGAC 
AATCCCAGCA GTGAAAGTGC CCTGAACGAA GAATGGCACT CAGATAACAG TGATGGTGAA 
ATTGCTAGTG AATGTGAATG CGATAGTGTC TTTAACCATT TAGAGGAACT GAGACTTCAT 
CTGGAGCAGG AAATGGGCTT TGAAAAATTC TTTGAGGTTT ATGAGAAAAT AAAGGCTATT 
CATGAAGATG AAGATGAAAA TATTGAAATT TGTTCAAAAA TAGTTCAAAA TATTTTGGGA 
AATGAACATC AGCATCTTTA TGCCAAGATT CTTCATTTAG TCATGGCAGA TGGAGCCTAC 
CAAGAAGATA ATGATGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( NM_001199397.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001186326.1 Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21522
Clone ID Related Accession (Same CDS sequence)	NM_001199397.3 , NM_001374418.1 , NM_001199397.1 , NM_001199397.2
Accession Version	NM_001199397.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-01
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA697159.1, AC084724.6, AB067488.1, AC116621.8, AC116615.5, AK025658.1 and AI984346.1. On Jun 2, 2019 this sequence version replaced NM_001199397.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.111804.1, CR933642.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTGGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG AGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT TCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGGAAT CCTGCAAAAC 
CTGGCAGCTA TGTATGGAGG CAGGCCCAGC TCTTCAAGAG GAGGGAAGCC AAGAAACAAA 
GAGGAAGAGG TTTATCTGGC AAGACTGAGG CAAATAAGAC TACAGAATTT CAATGAGCGC 
CAACAGATTA AAGCCAAACT TCGTGGTGAA AAGAAAGAAG CTAATCATTC TGAAGGACAA 
GAAGGAAGTG AAGAGGCTGA CATGAGGCGC AAAAAAATCG AATCACTGAA GGCCCATGCA 
AATGCACGTG CTGCTGTACT AAAAGAACAA CTAGAACGAA AGAGAAAGGA GGCTTATGAG 
AGAGAAAAAA AAGTGTGGGA AGAGCATTTG GTGGCTAAAG GAGTTAAGAG TTCTGATGTT 
TCTCCACCTT TGGGACAGCA TGAAACAGGT GGCTCTCCAT CAAAGCAACA GATGAGATCT 
GTTATTTCTG TAACTTCAGC TTTGAAAGAA GTTGGCGTGG ACAGTAGTTT AACTGATACC 
CGGGAAACTT CAGAAGAGAT GCAAAAGACC AACAATGCTA TTTCAAGTAA GCGAGAAATA 
CTTCGTAGAT TAAATGAAAA TCTTAAAGCT CAAGAAGATG AAAAAGGAAA GCAGAATCTC 
TCTGATACTT TTGAGATAAA TGTTCATGAA GATGCCAAAG AGCATGAAAA AGAAAAATCA 
GTTTCATCTG ATCGCAAGAA GTGGGAGGCA GGAGGTCAAC TTGTGATTCC TCTGGATGAG 
TTAACACTAG ATACATCCTT CTCTACAACT GAAAGACATA CAGTGGGAGA AGTTATTAAA 
TTAGGTCCTA ATGGATCTCC AAGAAGAGCC TGGGGGAAAA GTCCGACAGA TTCTGTTCTA 
AAGATACTTG GAGAAGCTGA ACTACAACTT CAGACAGAAC TATTAGAAAA TACAACTATT 
AGAAGTGAGA TTTCTCCCGA AGGGGAAAAG TACAAACCCT TAATTACTGG AGAAAAAAAA 
GTACAATGTA TTTCACATGA AATAAACCCA TCAGCTATTG TTGATTCTCC TGTTGAGACA 
AAAAGTCCCG AGTTCAGTGA GGCATCTCCA CAGATGTCAT TGAAACTGGA AGGAAATTTA 
GAAGAACCTG ATGATTTGGA AACAGAAATT CTACAAGAGC CAAGTGGAAC AAACAAAGAT 
GAGAGCTTGC CATGCACTAT TACTGATGTG TGGATTAGTG AGGAAAAAGA AACAAAGGAA 
ACTCAGTCGG CAGATAGGAT CACCATTCAG GAAAATGAAG TTTCTGAAGA TGGAGTCTCG 
AGTACTGTGG ACCAACTTAG TGACATTCAT ATAGAGCCTG GAACCAATGA TTCTCAGCAC 
TCTAAATGTG ATGTAGATAA GTCTGTGCAA CCGGAACCAT TTTTCCATAA GGTGGTTCAT 
TCTGAACACT TGAACTTAGT CCCTCAAGTT CAATCAGTTC AGTGTTCACC AGAAGAATCC 
TTTGCATTTC GATCTCACTC GCATTTACCA CCAAAAAATA AAAACAAGAA TTCCTTGCTG 
ATTGGACTTT CAACTGGTCT GTTTGATGCA AACAACCCAA AGATGTTAAG GACATGTTCA 
CTTCCAGATC TCTCAAAGCT GTTCAGAACC CTTATGGATG TTCCCACCGT AGGAGATGTT 
CGTCAAGACA ATCTTGAAAT AGATGAAATT GAAGATGAAA ACATTAAAGA AGGACCTTCT 
GATTCTGAAG ACATTGTGTT TGAAGAAACT GACACAGATT TACAAGAGCT GCAGGCCTCG 
ATGGAACAGT TACTTAGGGA ACAACCTGGT GAAGAATACA GTGAAGAAGA AGAGTCAGTC 
TTGAAGAACA GTGATGTGGA GCCAACTGCA AATGGGACAG ATGTGGCAGA TGAAGATGAC 
AATCCCAGCA GTGAAAGTGC CCTGAACGAA GAATGGCACT CAGATAACAG TGATGGTGAA 
ATTGCTAGTG AATGTGAATG CGATAGTGTC TTTAACCATT TAGAGGAACT GAGACTTCAT 
CTGGAGCAGG AAATGGGCTT TGAAAAATTC TTTGAGGTTT ATGAGAAAAT AAAGGCTATT 
CATGAAGATG AAGATGAAAA TATTGAAATT TGTTCAAAAA TAGTTCAAAA TATTTTGGGA 
AATGAACATC AGCATCTTTA TGCCAAGATT CTTCATTTAG TCATGGCAGA TGGAGCCTAC 
CAAGAAGATA ATGATGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186326.1
CDS	535..4395
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKPLQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERGILPGVRPGFPYGAAGHHHFPDADDIRKTLKRLKAVSKQANANRQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMGILQNLAAMYGGRPSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIKAKLRGEKKEANHSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQNLSDTFEINVHEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLGPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSEISPEGEKYKPLITGEKKVQCISHEINPSAIVDSPVETKSPEFSEASPQMSLKLEGNLEEPDDLETEILQEPSGTNKDESLPCTITDVWISEEKETKETQSADRITIQENEVSEDGVSSTVDQLSDIHIEPGTNDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGEIASECECDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	NM_001199397.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199397.2

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTGGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG AGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT TCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGGAAT CCTGCAAAAC 
CTGGCAGCTA TGTATGGAGG CAGGCCCAGC TCTTCAAGAG GAGGGAAGCC AAGAAACAAA 
GAGGAAGAGG TTTATCTGGC AAGACTGAGG CAAATAAGAC TACAGAATTT CAATGAGCGC 
CAACAGATTA AAGCCAAACT TCGTGGTGAA AAGAAAGAAG CTAATCATTC TGAAGGACAA 
GAAGGAAGTG AAGAGGCTGA CATGAGGCGC AAAAAAATCG AATCACTGAA GGCCCATGCA 
AATGCACGTG CTGCTGTACT AAAAGAACAA CTAGAACGAA AGAGAAAGGA GGCTTATGAG 
AGAGAAAAAA AAGTGTGGGA AGAGCATTTG GTGGCTAAAG GAGTTAAGAG TTCTGATGTT 
TCTCCACCTT TGGGACAGCA TGAAACAGGT GGCTCTCCAT CAAAGCAACA GATGAGATCT 
GTTATTTCTG TAACTTCAGC TTTGAAAGAA GTTGGCGTGG ACAGTAGTTT AACTGATACC 
CGGGAAACTT CAGAAGAGAT GCAAAAGACC AACAATGCTA TTTCAAGTAA GCGAGAAATA 
CTTCGTAGAT TAAATGAAAA TCTTAAAGCT CAAGAAGATG AAAAAGGAAA GCAGAATCTC 
TCTGATACTT TTGAGATAAA TGTTCATGAA GATGCCAAAG AGCATGAAAA AGAAAAATCA 
GTTTCATCTG ATCGCAAGAA GTGGGAGGCA GGAGGTCAAC TTGTGATTCC TCTGGATGAG 
TTAACACTAG ATACATCCTT CTCTACAACT GAAAGACATA CAGTGGGAGA AGTTATTAAA 
TTAGGTCCTA ATGGATCTCC AAGAAGAGCC TGGGGGAAAA GTCCGACAGA TTCTGTTCTA 
AAGATACTTG GAGAAGCTGA ACTACAACTT CAGACAGAAC TATTAGAAAA TACAACTATT 
AGAAGTGAGA TTTCTCCCGA AGGGGAAAAG TACAAACCCT TAATTACTGG AGAAAAAAAA 
GTACAATGTA TTTCACATGA AATAAACCCA TCAGCTATTG TTGATTCTCC TGTTGAGACA 
AAAAGTCCCG AGTTCAGTGA GGCATCTCCA CAGATGTCAT TGAAACTGGA AGGAAATTTA 
GAAGAACCTG ATGATTTGGA AACAGAAATT CTACAAGAGC CAAGTGGAAC AAACAAAGAT 
GAGAGCTTGC CATGCACTAT TACTGATGTG TGGATTAGTG AGGAAAAAGA AACAAAGGAA 
ACTCAGTCGG CAGATAGGAT CACCATTCAG GAAAATGAAG TTTCTGAAGA TGGAGTCTCG 
AGTACTGTGG ACCAACTTAG TGACATTCAT ATAGAGCCTG GAACCAATGA TTCTCAGCAC 
TCTAAATGTG ATGTAGATAA GTCTGTGCAA CCGGAACCAT TTTTCCATAA GGTGGTTCAT 
TCTGAACACT TGAACTTAGT CCCTCAAGTT CAATCAGTTC AGTGTTCACC AGAAGAATCC 
TTTGCATTTC GATCTCACTC GCATTTACCA CCAAAAAATA AAAACAAGAA TTCCTTGCTG 
ATTGGACTTT CAACTGGTCT GTTTGATGCA AACAACCCAA AGATGTTAAG GACATGTTCA 
CTTCCAGATC TCTCAAAGCT GTTCAGAACC CTTATGGATG TTCCCACCGT AGGAGATGTT 
CGTCAAGACA ATCTTGAAAT AGATGAAATT GAAGATGAAA ACATTAAAGA AGGACCTTCT 
GATTCTGAAG ACATTGTGTT TGAAGAAACT GACACAGATT TACAAGAGCT GCAGGCCTCG 
ATGGAACAGT TACTTAGGGA ACAACCTGGT GAAGAATACA GTGAAGAAGA AGAGTCAGTC 
TTGAAGAACA GTGATGTGGA GCCAACTGCA AATGGGACAG ATGTGGCAGA TGAAGATGAC 
AATCCCAGCA GTGAAAGTGC CCTGAACGAA GAATGGCACT CAGATAACAG TGATGGTGAA 
ATTGCTAGTG AATGTGAATG CGATAGTGTC TTTAACCATT TAGAGGAACT GAGACTTCAT 
CTGGAGCAGG AAATGGGCTT TGAAAAATTC TTTGAGGTTT ATGAGAAAAT AAAGGCTATT 
CATGAAGATG AAGATGAAAA TATTGAAATT TGTTCAAAAA TAGTTCAAAA TATTTTGGGA 
AATGAACATC AGCATCTTTA TGCCAAGATT CTTCATTTAG TCATGGCAGA TGGAGCCTAC 
CAAGAAGATA ATGATGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_011532003.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530305.1 Homo sapiens NIMA related kinase 1 (NEK1), transcript variant X1, mRNA.

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CloneID	OHu10748
Clone ID Related Accession (Same CDS sequence)	NM_012224.2 , XM_011532003.1 , NM_001374419.1 , NM_012224.3
Accession Version	XM_011532003.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTGGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG AGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT TCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGTTTA TCTGGCAAGA 
CTGAGGCAAA TAAGACTACA GAATTTCAAT GAGCGCCAAC AGATTAAAGC CAAACTTCGT 
GGTGAAAAGA AAGAAGCTAA TCATTCTGAA GGACAAGAAG GAAGTGAAGA GGCTGACATG 
AGGCGCAAAA AAATCGAATC ACTGAAGGCC CATGCAAATG CACGTGCTGC TGTACTAAAA 
GAACAACTAG AACGAAAGAG AAAGGAGGCT TATGAGAGAG AAAAAAAAGT GTGGGAAGAG 
CATTTGGTGG CTAAAGGAGT TAAGAGTTCT GATGTTTCTC CACCTTTGGG ACAGCATGAA 
ACAGGTGGCT CTCCATCAAA GCAACAGATG AGATCTGTTA TTTCTGTAAC TTCAGCTTTG 
AAAGAAGTTG GCGTGGACAG TAGTTTAACT GATACCCGGG AAACTTCAGA AGAGATGCAA 
AAGACCAACA ATGCTATTTC AAGTAAGCGA GAAATACTTC GTAGATTAAA TGAAAATCTT 
AAAGCTCAAG AAGATGAAAA AGGAAAGCAG AATCTCTCTG ATACTTTTGA GATAAATGTT 
CATGAAGATG CCAAAGAGCA TGAAAAAGAA AAATCAGTTT CATCTGATCG CAAGAAGTGG 
GAGGCAGGAG GTCAACTTGT GATTCCTCTG GATGAGTTAA CACTAGATAC ATCCTTCTCT 
ACAACTGAAA GACATACAGT GGGAGAAGTT ATTAAATTAG GTCCTAATGG ATCTCCAAGA 
AGAGCCTGGG GGAAAAGTCC GACAGATTCT GTTCTAAAGA TACTTGGAGA AGCTGAACTA 
CAACTTCAGA CAGAACTATT AGAAAATACA ACTATTAGAA GTGAGATTTC TCCCGAAGGG 
GAAAAGTACA AACCCTTAAT TACTGGAGAA AAAAAAGTAC AATGTATTTC ACATGAAATA 
AACCCATCAG CTATTGTTGA TTCTCCTGTT GAGACAAAAA GTCCCGAGTT CAGTGAGGCA 
TCTCCACAGA TGTCATTGAA ACTGGAAGGA AATTTAGAAG AACCTGATGA TTTGGAAACA 
GAAATTCTAC AAGAGCCAAG TGGAACAAAC AAAGATGAGA GCTTGCCATG CACTATTACT 
GATGTGTGGA TTAGTGAGGA AAAAGAAACA AAGGAAACTC AGTCGGCAGA TAGGATCACC 
ATTCAGGAAA ATGAAGTTTC TGAAGATGGA GTCTCGAGTA CTGTGGACCA ACTTAGTGAC 
ATTCATATAG AGCCTGGAAC CAATGATTCT CAGCACTCTA AATGTGATGT AGATAAGTCT 
GTGCAACCGG AACCATTTTT CCATAAGGTG GTTCATTCTG AACACTTGAA CTTAGTCCCT 
CAAGTTCAAT CAGTTCAGTG TTCACCAGAA GAATCCTTTG CATTTCGATC TCACTCGCAT 
TTACCACCAA AAAATAAAAA CAAGAATTCC TTGCTGATTG GACTTTCAAC TGGTCTGTTT 
GATGCAAACA ACCCAAAGAT GTTAAGGACA TGTTCACTTC CAGATCTCTC AAAGCTGTTC 
AGAACCCTTA TGGATGTTCC CACCGTAGGA GATGTTCGTC AAGACAATCT TGAAATAGAT 
GAAATTGAAG ATGAAAACAT TAAAGAAGGA CCTTCTGATT CTGAAGACAT TGTGTTTGAA 
GAAACTGACA CAGATTTACA AGAGCTGCAG GCCTCGATGG AACAGTTACT TAGGGAACAA 
CCTGGTGAAG AATACAGTGA AGAAGAAGAG TCAGTCTTGA AGAACAGTGA TGTGGAGCCA 
ACTGCAAATG GGACAGATGT GGCAGATGAA GATGACAATC CCAGCAGTGA AAGTGCCCTG 
AACGAAGAAT GGCACTCAGA TAACAGTGAT GGTGAAATTG CTAGTGAATG TGAATGCGAT 
AGTGTCTTTA ACCATTTAGA GGAACTGAGA CTTCATCTGG AGCAGGAAAT GGGCTTTGAA 
AAATTCTTTG AGGTTTATGA GAAAATAAAG GCTATTCATG AAGATGAAGA TGAAAATATT 
GAAATTTGTT CAAAAATAGT TCAAAATATT TTGGGAAATG AACATCAGCA TCTTTATGCC 
AAGATTCTTC ATTTAGTCAT GGCAGATGGA GCCTACCAAG AAGATAATGA TGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530305.1
CDS	581..4357
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKPLQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERGILPGVRPGFPYGAAGHHHFPDADDIRKTLKRLKAVSKQANANRQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMVYLARLRQIRLQNFNERQQIKAKLRGEKKEANHSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQNLSDTFEINVHEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLGPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSEISPEGEKYKPLITGEKKVQCISHEINPSAIVDSPVETKSPEFSEASPQMSLKLEGNLEEPDDLETEILQEPSGTNKDESLPCTITDVWISEEKETKETQSADRITIQENEVSEDGVSSTVDQLSDIHIEPGTNDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGEIASECECDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_011532003.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532003.1

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTGGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG AGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT TCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGTTTA TCTGGCAAGA 
CTGAGGCAAA TAAGACTACA GAATTTCAAT GAGCGCCAAC AGATTAAAGC CAAACTTCGT 
GGTGAAAAGA AAGAAGCTAA TCATTCTGAA GGACAAGAAG GAAGTGAAGA GGCTGACATG 
AGGCGCAAAA AAATCGAATC ACTGAAGGCC CATGCAAATG CACGTGCTGC TGTACTAAAA 
GAACAACTAG AACGAAAGAG AAAGGAGGCT TATGAGAGAG AAAAAAAAGT GTGGGAAGAG 
CATTTGGTGG CTAAAGGAGT TAAGAGTTCT GATGTTTCTC CACCTTTGGG ACAGCATGAA 
ACAGGTGGCT CTCCATCAAA GCAACAGATG AGATCTGTTA TTTCTGTAAC TTCAGCTTTG 
AAAGAAGTTG GCGTGGACAG TAGTTTAACT GATACCCGGG AAACTTCAGA AGAGATGCAA 
AAGACCAACA ATGCTATTTC AAGTAAGCGA GAAATACTTC GTAGATTAAA TGAAAATCTT 
AAAGCTCAAG AAGATGAAAA AGGAAAGCAG AATCTCTCTG ATACTTTTGA GATAAATGTT 
CATGAAGATG CCAAAGAGCA TGAAAAAGAA AAATCAGTTT CATCTGATCG CAAGAAGTGG 
GAGGCAGGAG GTCAACTTGT GATTCCTCTG GATGAGTTAA CACTAGATAC ATCCTTCTCT 
ACAACTGAAA GACATACAGT GGGAGAAGTT ATTAAATTAG GTCCTAATGG ATCTCCAAGA 
AGAGCCTGGG GGAAAAGTCC GACAGATTCT GTTCTAAAGA TACTTGGAGA AGCTGAACTA 
CAACTTCAGA CAGAACTATT AGAAAATACA ACTATTAGAA GTGAGATTTC TCCCGAAGGG 
GAAAAGTACA AACCCTTAAT TACTGGAGAA AAAAAAGTAC AATGTATTTC ACATGAAATA 
AACCCATCAG CTATTGTTGA TTCTCCTGTT GAGACAAAAA GTCCCGAGTT CAGTGAGGCA 
TCTCCACAGA TGTCATTGAA ACTGGAAGGA AATTTAGAAG AACCTGATGA TTTGGAAACA 
GAAATTCTAC AAGAGCCAAG TGGAACAAAC AAAGATGAGA GCTTGCCATG CACTATTACT 
GATGTGTGGA TTAGTGAGGA AAAAGAAACA AAGGAAACTC AGTCGGCAGA TAGGATCACC 
ATTCAGGAAA ATGAAGTTTC TGAAGATGGA GTCTCGAGTA CTGTGGACCA ACTTAGTGAC 
ATTCATATAG AGCCTGGAAC CAATGATTCT CAGCACTCTA AATGTGATGT AGATAAGTCT 
GTGCAACCGG AACCATTTTT CCATAAGGTG GTTCATTCTG AACACTTGAA CTTAGTCCCT 
CAAGTTCAAT CAGTTCAGTG TTCACCAGAA GAATCCTTTG CATTTCGATC TCACTCGCAT 
TTACCACCAA AAAATAAAAA CAAGAATTCC TTGCTGATTG GACTTTCAAC TGGTCTGTTT 
GATGCAAACA ACCCAAAGAT GTTAAGGACA TGTTCACTTC CAGATCTCTC AAAGCTGTTC 
AGAACCCTTA TGGATGTTCC CACCGTAGGA GATGTTCGTC AAGACAATCT TGAAATAGAT 
GAAATTGAAG ATGAAAACAT TAAAGAAGGA CCTTCTGATT CTGAAGACAT TGTGTTTGAA 
GAAACTGACA CAGATTTACA AGAGCTGCAG GCCTCGATGG AACAGTTACT TAGGGAACAA 
CCTGGTGAAG AATACAGTGA AGAAGAAGAG TCAGTCTTGA AGAACAGTGA TGTGGAGCCA 
ACTGCAAATG GGACAGATGT GGCAGATGAA GATGACAATC CCAGCAGTGA AAGTGCCCTG 
AACGAAGAAT GGCACTCAGA TAACAGTGAT GGTGAAATTG CTAGTGAATG TGAATGCGAT 
AGTGTCTTTA ACCATTTAGA GGAACTGAGA CTTCATCTGG AGCAGGAAAT GGGCTTTGAA 
AAATTCTTTG AGGTTTATGA GAAAATAAAG GCTATTCATG AAGATGAAGA TGAAAATATT 
GAAATTTGTT CAAAAATAGT TCAAAATATT TTGGGAAATG AACATCAGCA TCTTTATGCC 
AAGATTCTTC ATTTAGTCAT GGCAGATGGA GCCTACCAAG AAGATAATGA TGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( NM_001374423.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001361352.1 Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 11, mRNA.

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CloneID	OHu109294
Clone ID Related Accession (Same CDS sequence)	NM_001374423.1 , NM_001374422.1
Accession Version	NM_001374423.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek1 isoform 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC084724.6. Transcript Variant: This variant (11), as well as variant 10, encodes isoform 8. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.378.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGCCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001361352.1
CDS	613..1884
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKPLQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKP

Target ORF information:

RefSeq Version	NM_001374423.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001374423.1

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGCCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( NM_001374422.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001361351.1 Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 10, mRNA.

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CloneID	OHu109294
Clone ID Related Accession (Same CDS sequence)	NM_001374423.1 , NM_001374422.1
Accession Version	NM_001374422.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek1 isoform 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC084724.6. Transcript Variant: This variant (10), as well as variant 11, encodes isoform 8. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.10612.1, SRR1660803.112243.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGCCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001361351.1
CDS	535..1806
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKPLQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKP

Target ORF information:

RefSeq Version	NM_001374422.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001374422.1

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGCCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_017008253.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863742.1 Homo sapiens NIMA related kinase 1 (NEK1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu95207
Clone ID Related Accession (Same CDS sequence)	XM_017008253.1
Accession Version	XM_017008253.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2709bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCTG AACAAATGAA AAGGCAAGAA AAGGAAAGGT TGGAAAGAAT AAATAGGGCC 
AGGGAACAAG GATGGAGAAA TGTGCTAAGT GCTGGTGGAA GTGGTGAAGT AAAGGCTCCT 
TTTCTGGGCA GTGGAGGGAC TATAGCTCCA TCATCTTTTT CTTCTCGAGG ACAGTATGAA 
CATTACCATG CCATTTTTGA CCAAATGCAG CAACAAAGAG CAGAAGATAA TGAAGCTAAA 
TGGAAAAGAG AAATATATGG TCGAGGTCTT CCAGAAAGAG GAATTCTGCC TGGAGTTCGT 
CCAGGATTTC CTTATGGGGC TGCAGGTCAT CACCATTTTC CTGATGCTGA TGATATTAGA 
AAAACTTTGA AAAGATTGAA GGCGGTGTCT AAACAAGCCA ATGCAAACAG GCAAAAAGGG 
CAGCTAGCTG TAGAAAGAGC TAAACAAGTA GAAGAGTTCC TGCAGCGAAA ACGGGAAGCT 
ATGCAGAATA AAGCTCGAGC CGAAGGACAT ATGGGAATCC TGCAAAACCT GGCAGCTATG 
TATGGAGGCA GGCCCAGCTC TTCAAGAGGA GGGAAGCCAA GAAACAAAGA GGAAGAGGTT 
TATCTGGCAA GACTGAGGCA AATAAGACTA CAGAATTTCA ATGAGCGCCA ACAGATTAAA 
GCCAAACTTC GTGGTGAAAA GAAAGAAGCT AATCATTCTG AAGGACAAGA AGGAAGTGAA 
GAGGCTGACA TGAGGCGCAA AAAAATCGAA TCACTGAAGG CCCATGCAAA TGCACGTGCT 
GCTGTACTAA AAGAACAACT AGAACGAAAG AGAAAGGAGG CTTATGAGAG AGAAAAAAAA 
GTGTGGGAAG AGCATTTGGT GGCTAAAGGA GTTAAGAGTT CTGATGTTTC TCCACCTTTG 
GGACAGCATG AAACAGGTGG CTCTCCATCA AAGCAACAGA TGAGATCTGT TATTTCTGTA 
ACTTCAGCTT TGAAAGAAGT TGGCGTGGAC AGTAGTTTAA CTGATACCCG GGAAACTTCA 
GAAGAGATGC AAAAGACCAA CAATGCTATT TCAAGTAAGC GAGAAATACT TCGTAGATTA 
AATGAAAATC TTAAAGCTCA AGAAGATGAA AAAGGAAAGC AGAATCTCTC TGATACTTTT 
GAGATAAATG TTCATGAAGA TGCCAAAGAG CATGAAAAAG AAAAATCAGT TTCATCTGAT 
CGCAAGAAGT GGGAGGCAGG AGGTCAACTT GTGATTCCTC TGGATGAGTT AACACTAGAT 
ACATCCTTCT CTACAACTGA AAGACATACA GTGGGAGAAG TTATTAAATT AGGTCCTAAT 
GGATCTCCAA GAAGAGCCTG GGGGAAAAGT CCGACAGATT CTGTTCTAAA GATACTTGGA 
GAAGCTGAAC TACAACTTCA GACAGAACTA TTAGAAAATA CAACTATTAG AAGTGAGATT 
TCTCCCGAAG GGGAAAAGTA CAAACCCTTA ATTACTGGAG AAAAAAAAGT ACAATGTATT 
TCACATGAAA TAAACCCATC AGCTATTGTT GATTCTCCTG TTGAGACAAA AAGTCCCGAG 
TTCAGTGAGG CATCTCCACA GATGTCATTG AAACTGGAAG GAAATTTAGA AGAACCTGAT 
GATTTGGAAA CAGAAATTCT ACAAGAGCCA AGTGGAACAA ACAAAGATGA GAGCTTGCCA 
TGCACTATTA CTGATGTGTG GATTAGTGAG GAAAAAGAAA CAAAGGAAAC TCAGTCGGCA 
GATAGGATCA CCATTCAGGA AAATGAAGTT TCTGAAGATG GAGTCTCGAG TACTGTGGAC 
CAACTTAGTG ACATTCATAT AGAGCCTGGA ACCAATGATT CTCAGCACTC TAAATGTGAT 
GTAGATAAGT CTGTGCAACC GGAACCATTT TTCCATAAGG TGGTTCATTC TGAACACTTG 
AACTTAGTCC CTCAAGTTCA ATCAGTTCAG TGTTCACCAG AAGAATCCTT TGCATTTCGA 
TCTCACTCGC ATTTACCACC AAAAAATAAA AACAAGAATT CCTTGCTGAT TGGACTTTCA 
ACTGGTCTGT TTGATGCAAA CAACCCAAAG ATGTTAAGGA CATGTTCACT TCCAGATCTC 
TCAAAGCTGT TCAGAACCCT TATGGATGTT CCCACCGTAG GAGATGTTCG TCAAGACAAT 
CTTGAAATAG ATGAAATTGA AGATGAAAAC ATTAAAGAAG GACCTTCTGA TTCTGAAGAC 
ATTGTGTTTG AAGAAACTGA CACAGATTTA CAAGAGCTGC AGGCCTCGAT GGAACAGTTA 
CTTAGGGAAC AACCTGGTGA AGAATACAGT GAAGAAGAAG AGTCAGTCTT GAAGAACAGT 
GATGTGGAGC CAACTGCAAA TGGGACAGAT GTGGCAGATG AAGATGACAA TCCCAGCAGT 
GAAAGTGCCC TGAACGAAGA ATGGCACTCA GATAACAGTG ATGGTGAAAT TGCTAGTGAA 
TGTGAATGCG ATAGTGTCTT TAACCATTTA GAGGAACTGA GACTTCATCT GGAGCAGGAA 
ATGGGCTTTG AAAAATTCTT TGAGGTTTAT GAGAAAATAA AGGCTATTCA TGAAGATGAA 
GATGAAAATA TTGAAATTTG TTCAAAAATA GTTCAAAATA TTTTGGGAAA TGAACATCAG 
CATCTTTATG CCAAGATTCT TCATTTAGTC ATGGCAGATG GAGCCTACCA AGAAGATAAT 
GATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863742.1
CDS	1581..4289
Translation	MKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERGILPGVRPGFPYGAAGHHHFPDADDIRKTLKRLKAVSKQANANRQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMGILQNLAAMYGGRPSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIKAKLRGEKKEANHSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQNLSDTFEINVHEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLGPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSEISPEGEKYKPLITGEKKVQCISHEINPSAIVDSPVETKSPEFSEASPQMSLKLEGNLEEPDDLETEILQEPSGTNKDESLPCTITDVWISEEKETKETQSADRITIQENEVSEDGVSSTVDQLSDIHIEPGTNDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGEIASECECDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_017008253.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008253.1

ORF Insert Sequence:

ATGAAGGCTG AACAAATGAA AAGGCAAGAA AAGGAAAGGT TGGAAAGAAT AAATAGGGCC 
AGGGAACAAG GATGGAGAAA TGTGCTAAGT GCTGGTGGAA GTGGTGAAGT AAAGGCTCCT 
TTTCTGGGCA GTGGAGGGAC TATAGCTCCA TCATCTTTTT CTTCTCGAGG ACAGTATGAA 
CATTACCATG CCATTTTTGA CCAAATGCAG CAACAAAGAG CAGAAGATAA TGAAGCTAAA 
TGGAAAAGAG AAATATATGG TCGAGGTCTT CCAGAAAGAG GAATTCTGCC TGGAGTTCGT 
CCAGGATTTC CTTATGGGGC TGCAGGTCAT CACCATTTTC CTGATGCTGA TGATATTAGA 
AAAACTTTGA AAAGATTGAA GGCGGTGTCT AAACAAGCCA ATGCAAACAG GCAAAAAGGG 
CAGCTAGCTG TAGAAAGAGC TAAACAAGTA GAAGAGTTCC TGCAGCGAAA ACGGGAAGCT 
ATGCAGAATA AAGCTCGAGC CGAAGGACAT ATGGGAATCC TGCAAAACCT GGCAGCTATG 
TATGGAGGCA GGCCCAGCTC TTCAAGAGGA GGGAAGCCAA GAAACAAAGA GGAAGAGGTT 
TATCTGGCAA GACTGAGGCA AATAAGACTA CAGAATTTCA ATGAGCGCCA ACAGATTAAA 
GCCAAACTTC GTGGTGAAAA GAAAGAAGCT AATCATTCTG AAGGACAAGA AGGAAGTGAA 
GAGGCTGACA TGAGGCGCAA AAAAATCGAA TCACTGAAGG CCCATGCAAA TGCACGTGCT 
GCTGTACTAA AAGAACAACT AGAACGAAAG AGAAAGGAGG CTTATGAGAG AGAAAAAAAA 
GTGTGGGAAG AGCATTTGGT GGCTAAAGGA GTTAAGAGTT CTGATGTTTC TCCACCTTTG 
GGACAGCATG AAACAGGTGG CTCTCCATCA AAGCAACAGA TGAGATCTGT TATTTCTGTA 
ACTTCAGCTT TGAAAGAAGT TGGCGTGGAC AGTAGTTTAA CTGATACCCG GGAAACTTCA 
GAAGAGATGC AAAAGACCAA CAATGCTATT TCAAGTAAGC GAGAAATACT TCGTAGATTA 
AATGAAAATC TTAAAGCTCA AGAAGATGAA AAAGGAAAGC AGAATCTCTC TGATACTTTT 
GAGATAAATG TTCATGAAGA TGCCAAAGAG CATGAAAAAG AAAAATCAGT TTCATCTGAT 
CGCAAGAAGT GGGAGGCAGG AGGTCAACTT GTGATTCCTC TGGATGAGTT AACACTAGAT 
ACATCCTTCT CTACAACTGA AAGACATACA GTGGGAGAAG TTATTAAATT AGGTCCTAAT 
GGATCTCCAA GAAGAGCCTG GGGGAAAAGT CCGACAGATT CTGTTCTAAA GATACTTGGA 
GAAGCTGAAC TACAACTTCA GACAGAACTA TTAGAAAATA CAACTATTAG AAGTGAGATT 
TCTCCCGAAG GGGAAAAGTA CAAACCCTTA ATTACTGGAG AAAAAAAAGT ACAATGTATT 
TCACATGAAA TAAACCCATC AGCTATTGTT GATTCTCCTG TTGAGACAAA AAGTCCCGAG 
TTCAGTGAGG CATCTCCACA GATGTCATTG AAACTGGAAG GAAATTTAGA AGAACCTGAT 
GATTTGGAAA CAGAAATTCT ACAAGAGCCA AGTGGAACAA ACAAAGATGA GAGCTTGCCA 
TGCACTATTA CTGATGTGTG GATTAGTGAG GAAAAAGAAA CAAAGGAAAC TCAGTCGGCA 
GATAGGATCA CCATTCAGGA AAATGAAGTT TCTGAAGATG GAGTCTCGAG TACTGTGGAC 
CAACTTAGTG ACATTCATAT AGAGCCTGGA ACCAATGATT CTCAGCACTC TAAATGTGAT 
GTAGATAAGT CTGTGCAACC GGAACCATTT TTCCATAAGG TGGTTCATTC TGAACACTTG 
AACTTAGTCC CTCAAGTTCA ATCAGTTCAG TGTTCACCAG AAGAATCCTT TGCATTTCGA 
TCTCACTCGC ATTTACCACC AAAAAATAAA AACAAGAATT CCTTGCTGAT TGGACTTTCA 
ACTGGTCTGT TTGATGCAAA CAACCCAAAG ATGTTAAGGA CATGTTCACT TCCAGATCTC 
TCAAAGCTGT TCAGAACCCT TATGGATGTT CCCACCGTAG GAGATGTTCG TCAAGACAAT 
CTTGAAATAG ATGAAATTGA AGATGAAAAC ATTAAAGAAG GACCTTCTGA TTCTGAAGAC 
ATTGTGTTTG AAGAAACTGA CACAGATTTA CAAGAGCTGC AGGCCTCGAT GGAACAGTTA 
CTTAGGGAAC AACCTGGTGA AGAATACAGT GAAGAAGAAG AGTCAGTCTT GAAGAACAGT 
GATGTGGAGC CAACTGCAAA TGGGACAGAT GTGGCAGATG AAGATGACAA TCCCAGCAGT 
GAAAGTGCCC TGAACGAAGA ATGGCACTCA GATAACAGTG ATGGTGAAAT TGCTAGTGAA 
TGTGAATGCG ATAGTGTCTT TAACCATTTA GAGGAACTGA GACTTCATCT GGAGCAGGAA 
ATGGGCTTTG AAAAATTCTT TGAGGTTTAT GAGAAAATAA AGGCTATTCA TGAAGATGAA 
GATGAAAATA TTGAAATTTG TTCAAAAATA GTTCAAAATA TTTTGGGAAA TGAACATCAG 
CATCTTTATG CCAAGATTCT TCATTTAGTC ATGGCAGATG GAGCCTACCA AGAAGATAAT 
GATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_017008254.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863743.1 Homo sapiens NIMA related kinase 1 (NEK1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu95208
Clone ID Related Accession (Same CDS sequence)	XM_017008254.1
Accession Version	XM_017008254.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCAAC AAAGAGCAGA AGATAATGAA GCTAAATGGA AAAGAGAAAT ATATGGTCGA 
GGTCTTCCAG AAAGAGGAAT TCTGCCTGGA GTTCGTCCAG GATTTCCTTA TGGGGCTGCA 
GGTCATCACC ATTTTCCTGA TGCTGATGAT ATTAGAAAAA CTTTGAAAAG ATTGAAGGCG 
GTGTCTAAAC AAGCCAATGC AAACAGGCAA AAAGGGCAGC TAGCTGTAGA AAGAGCTAAA 
CAAGTAGAAG AGTTCCTGCA GCGAAAACGG GAAGCTATGC AGAATAAAGC TCGAGCCGAA 
GGACATATGG GAATCCTGCA AAACCTGGCA GCTATGTATG GAGGCAGGCC CAGCTCTTCA 
AGAGGAGGGA AGCCAAGAAA CAAAGAGGAA GAGGTTTATC TGGCAAGACT GAGGCAAATA 
AGACTACAGA ATTTCAATGA GCGCCAACAG ATTAAAGCCA AACTTCGTGG TGAAAAGAAA 
GAAGCTAATC ATTCTGAAGG ACAAGAAGGA AGTGAAGAGG CTGACATGAG GCGCAAAAAA 
ATCGAATCAC TGAAGGCCCA TGCAAATGCA CGTGCTGCTG TACTAAAAGA ACAACTAGAA 
CGAAAGAGAA AGGAGGCTTA TGAGAGAGAA AAAAAAGTGT GGGAAGAGCA TTTGGTGGCT 
AAAGGAGTTA AGAGTTCTGA TGTTTCTCCA CCTTTGGGAC AGCATGAAAC AGGTGGCTCT 
CCATCAAAGC AACAGATGAG ATCTGTTATT TCTGTAACTT CAGCTTTGAA AGAAGTTGGC 
GTGGACAGTA GTTTAACTGA TACCCGGGAA ACTTCAGAAG AGATGCAAAA GACCAACAAT 
GCTATTTCAA GTAAGCGAGA AATACTTCGT AGATTAAATG AAAATCTTAA AGCTCAAGAA 
GATGAAAAAG GAAAGCAGAA TCTCTCTGAT ACTTTTGAGA TAAATGTTCA TGAAGATGCC 
AAAGAGCATG AAAAAGAAAA ATCAGTTTCA TCTGATCGCA AGAAGTGGGA GGCAGGAGGT 
CAACTTGTGA TTCCTCTGGA TGAGTTAACA CTAGATACAT CCTTCTCTAC AACTGAAAGA 
CATACAGTGG GAGAAGTTAT TAAATTAGGT CCTAATGGAT CTCCAAGAAG AGCCTGGGGG 
AAAAGTCCGA CAGATTCTGT TCTAAAGATA CTTGGAGAAG CTGAACTACA ACTTCAGACA 
GAACTATTAG AAAATACAAC TATTAGAAGT GAGATTTCTC CCGAAGGGGA AAAGTACAAA 
CCCTTAATTA CTGGAGAAAA AAAAGTACAA TGTATTTCAC ATGAAATAAA CCCATCAGCT 
ATTGTTGATT CTCCTGTTGA GACAAAAAGT CCCGAGTTCA GTGAGGCATC TCCACAGATG 
TCATTGAAAC TGGAAGGAAA TTTAGAAGAA CCTGATGATT TGGAAACAGA AATTCTACAA 
GAGCCAAGTG GAACAAACAA AGATGAGAGC TTGCCATGCA CTATTACTGA TGTGTGGATT 
AGTGAGGAAA AAGAAACAAA GGAAACTCAG TCGGCAGATA GGATCACCAT TCAGGAAAAT 
GAAGTTTCTG AAGATGGAGT CTCGAGTACT GTGGACCAAC TTAGTGACAT TCATATAGAG 
CCTGGAACCA ATGATTCTCA GCACTCTAAA TGTGATGTAG ATAAGTCTGT GCAACCGGAA 
CCATTTTTCC ATAAGGTGGT TCATTCTGAA CACTTGAACT TAGTCCCTCA AGTTCAATCA 
GTTCAGTGTT CACCAGAAGA ATCCTTTGCA TTTCGATCTC ACTCGCATTT ACCACCAAAA 
AATAAAAACA AGAATTCCTT GCTGATTGGA CTTTCAACTG GTCTGTTTGA TGCAAACAAC 
CCAAAGATGT TAAGGACATG TTCACTTCCA GATCTCTCAA AGCTGTTCAG AACCCTTATG 
GATGTTCCCA CCGTAGGAGA TGTTCGTCAA GACAATCTTG AAATAGATGA AATTGAAGAT 
GAAAACATTA AAGAAGGACC TTCTGATTCT GAAGACATTG TGTTTGAAGA AACTGACACA 
GATTTACAAG AGCTGCAGGC CTCGATGGAA CAGTTACTTA GGGAACAACC TGGTGAAGAA 
TACAGTGAAG AAGAAGAGTC AGTCTTGAAG AACAGTGATG TGGAGCCAAC TGCAAATGGG 
ACAGATGTGG CAGATGAAGA TGACAATCCC AGCAGTGAAA GTGCCCTGAA CGAAGAATGG 
CACTCAGATA ACAGTGATGG TGAAATTGCT AGTGAATGTG AATGCGATAG TGTCTTTAAC 
CATTTAGAGG AACTGAGACT TCATCTGGAG CAGGAAATGG GCTTTGAAAA ATTCTTTGAG 
GTTTATGAGA AAATAAAGGC TATTCATGAA GATGAAGATG AAAATATTGA AATTTGTTCA 
AAAATAGTTC AAAATATTTT GGGAAATGAA CATCAGCATC TTTATGCCAA GATTCTTCAT 
TTAGTCATGG CAGATGGAGC CTACCAAGAA GATAATGATG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863743.1
CDS	895..3399
Translation	MQQQRAEDNEAKWKREIYGRGLPERGILPGVRPGFPYGAAGHHHFPDADDIRKTLKRLKAVSKQANANRQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMGILQNLAAMYGGRPSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIKAKLRGEKKEANHSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQNLSDTFEINVHEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLGPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSEISPEGEKYKPLITGEKKVQCISHEINPSAIVDSPVETKSPEFSEASPQMSLKLEGNLEEPDDLETEILQEPSGTNKDESLPCTITDVWISEEKETKETQSADRITIQENEVSEDGVSSTVDQLSDIHIEPGTNDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGEIASECECDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_017008254.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008254.1

ORF Insert Sequence:

ATGCAGCAAC AAAGAGCAGA AGATAATGAA GCTAAATGGA AAAGAGAAAT ATATGGTCGA 
GGTCTTCCAG AAAGAGGAAT TCTGCCTGGA GTTCGTCCAG GATTTCCTTA TGGGGCTGCA 
GGTCATCACC ATTTTCCTGA TGCTGATGAT ATTAGAAAAA CTTTGAAAAG ATTGAAGGCG 
GTGTCTAAAC AAGCCAATGC AAACAGGCAA AAAGGGCAGC TAGCTGTAGA AAGAGCTAAA 
CAAGTAGAAG AGTTCCTGCA GCGAAAACGG GAAGCTATGC AGAATAAAGC TCGAGCCGAA 
GGACATATGG GAATCCTGCA AAACCTGGCA GCTATGTATG GAGGCAGGCC CAGCTCTTCA 
AGAGGAGGGA AGCCAAGAAA CAAAGAGGAA GAGGTTTATC TGGCAAGACT GAGGCAAATA 
AGACTACAGA ATTTCAATGA GCGCCAACAG ATTAAAGCCA AACTTCGTGG TGAAAAGAAA 
GAAGCTAATC ATTCTGAAGG ACAAGAAGGA AGTGAAGAGG CTGACATGAG GCGCAAAAAA 
ATCGAATCAC TGAAGGCCCA TGCAAATGCA CGTGCTGCTG TACTAAAAGA ACAACTAGAA 
CGAAAGAGAA AGGAGGCTTA TGAGAGAGAA AAAAAAGTGT GGGAAGAGCA TTTGGTGGCT 
AAAGGAGTTA AGAGTTCTGA TGTTTCTCCA CCTTTGGGAC AGCATGAAAC AGGTGGCTCT 
CCATCAAAGC AACAGATGAG ATCTGTTATT TCTGTAACTT CAGCTTTGAA AGAAGTTGGC 
GTGGACAGTA GTTTAACTGA TACCCGGGAA ACTTCAGAAG AGATGCAAAA GACCAACAAT 
GCTATTTCAA GTAAGCGAGA AATACTTCGT AGATTAAATG AAAATCTTAA AGCTCAAGAA 
GATGAAAAAG GAAAGCAGAA TCTCTCTGAT ACTTTTGAGA TAAATGTTCA TGAAGATGCC 
AAAGAGCATG AAAAAGAAAA ATCAGTTTCA TCTGATCGCA AGAAGTGGGA GGCAGGAGGT 
CAACTTGTGA TTCCTCTGGA TGAGTTAACA CTAGATACAT CCTTCTCTAC AACTGAAAGA 
CATACAGTGG GAGAAGTTAT TAAATTAGGT CCTAATGGAT CTCCAAGAAG AGCCTGGGGG 
AAAAGTCCGA CAGATTCTGT TCTAAAGATA CTTGGAGAAG CTGAACTACA ACTTCAGACA 
GAACTATTAG AAAATACAAC TATTAGAAGT GAGATTTCTC CCGAAGGGGA AAAGTACAAA 
CCCTTAATTA CTGGAGAAAA AAAAGTACAA TGTATTTCAC ATGAAATAAA CCCATCAGCT 
ATTGTTGATT CTCCTGTTGA GACAAAAAGT CCCGAGTTCA GTGAGGCATC TCCACAGATG 
TCATTGAAAC TGGAAGGAAA TTTAGAAGAA CCTGATGATT TGGAAACAGA AATTCTACAA 
GAGCCAAGTG GAACAAACAA AGATGAGAGC TTGCCATGCA CTATTACTGA TGTGTGGATT 
AGTGAGGAAA AAGAAACAAA GGAAACTCAG TCGGCAGATA GGATCACCAT TCAGGAAAAT 
GAAGTTTCTG AAGATGGAGT CTCGAGTACT GTGGACCAAC TTAGTGACAT TCATATAGAG 
CCTGGAACCA ATGATTCTCA GCACTCTAAA TGTGATGTAG ATAAGTCTGT GCAACCGGAA 
CCATTTTTCC ATAAGGTGGT TCATTCTGAA CACTTGAACT TAGTCCCTCA AGTTCAATCA 
GTTCAGTGTT CACCAGAAGA ATCCTTTGCA TTTCGATCTC ACTCGCATTT ACCACCAAAA 
AATAAAAACA AGAATTCCTT GCTGATTGGA CTTTCAACTG GTCTGTTTGA TGCAAACAAC 
CCAAAGATGT TAAGGACATG TTCACTTCCA GATCTCTCAA AGCTGTTCAG AACCCTTATG 
GATGTTCCCA CCGTAGGAGA TGTTCGTCAA GACAATCTTG AAATAGATGA AATTGAAGAT 
GAAAACATTA AAGAAGGACC TTCTGATTCT GAAGACATTG TGTTTGAAGA AACTGACACA 
GATTTACAAG AGCTGCAGGC CTCGATGGAA CAGTTACTTA GGGAACAACC TGGTGAAGAA 
TACAGTGAAG AAGAAGAGTC AGTCTTGAAG AACAGTGATG TGGAGCCAAC TGCAAATGGG 
ACAGATGTGG CAGATGAAGA TGACAATCCC AGCAGTGAAA GTGCCCTGAA CGAAGAATGG 
CACTCAGATA ACAGTGATGG TGAAATTGCT AGTGAATGTG AATGCGATAG TGTCTTTAAC 
CATTTAGAGG AACTGAGACT TCATCTGGAG CAGGAAATGG GCTTTGAAAA ATTCTTTGAG 
GTTTATGAGA AAATAAAGGC TATTCATGAA GATGAAGATG AAAATATTGA AATTTGTTCA 
AAAATAGTTC AAAATATTTT GGGAAATGAA CATCAGCATC TTTATGCCAA GATTCTTCAT 
TTAGTCATGG CAGATGGAGC CTACCAAGAA GATAATGATG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_011532005.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530307.1 Homo sapiens NIMA related kinase 1 (NEK1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu59243
Clone ID Related Accession (Same CDS sequence)	XM_011532005.2
Accession Version	XM_011532005.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011532005.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTGGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG AGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT TCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGGAAT CCTGCAAAAC 
CTGGCAGCTA TGTATGGAGG CAGGCCCAGC TCTTCAAGAG GAGGGAAGCC AAGAAACAAA 
GAGGAAGAGG TTTATCTGGC AAGACTGAGG CAAATAAGAC TACAGAATTT CAATGAGCGC 
CAACAGATTA AAGCCAAACT TCGTGGTGAA AAGAAAGAAG CTAATCATTC TGAAGGACAA 
GAAGGAAGTG AAGAGGCTGA CATGAGGCGC AAAAAAATCG AATCACTGAA GGCCCATGCA 
AATGCACGTG CTGCTGTACT AAAAGAACAA CTAGAACGAA AGAGAAAGGA GGCTTATGAG 
AGAGAAAAAA AAGTGTGGGA AGAGCATTTG GTGGCTAAAG GAGTTAAGAG TTCTGATGTT 
TCTCCACCTT TGGGACAGCA TGAAACAGGT GGCTCTCCAT CAAAGCAACA GATGAGATCT 
GTTATTTCTG TAACTTCAGC TTTGAAAGAA GTTGGCGTGG ACTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530307.1
CDS	581..2728
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKPLQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERGILPGVRPGFPYGAAGHHHFPDADDIRKTLKRLKAVSKQANANRQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMGILQNLAAMYGGRPSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIKAKLRGEKKEANHSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDC

Target ORF information:

RefSeq Version	XM_011532005.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532005.2

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTGGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG AGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT TCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGGAAT CCTGCAAAAC 
CTGGCAGCTA TGTATGGAGG CAGGCCCAGC TCTTCAAGAG GAGGGAAGCC AAGAAACAAA 
GAGGAAGAGG TTTATCTGGC AAGACTGAGG CAAATAAGAC TACAGAATTT CAATGAGCGC 
CAACAGATTA AAGCCAAACT TCGTGGTGAA AAGAAAGAAG CTAATCATTC TGAAGGACAA 
GAAGGAAGTG AAGAGGCTGA CATGAGGCGC AAAAAAATCG AATCACTGAA GGCCCATGCA 
AATGCACGTG CTGCTGTACT AAAAGAACAA CTAGAACGAA AGAGAAAGGA GGCTTATGAG 
AGAGAAAAAA AAGTGTGGGA AGAGCATTTG GTGGCTAAAG GAGTTAAGAG TTCTGATGTT 
TCTCCACCTT TGGGACAGCA TGAAACAGGT GGCTCTCCAT CAAAGCAACA GATGAGATCT 
GTTATTTCTG TAACTTCAGC TTTGAAAGAA GTTGGCGTGG ACTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_017008250.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863739.1 Homo sapiens NIMA related kinase 1 (NEK1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu95205
Clone ID Related Accession (Same CDS sequence)	XM_017008250.1 , XM_017008249.1 , XM_024454065.1
Accession Version	XM_017008250.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAACC TGGTACTGAA GATAATATCT GGATCTTTTC CACCTGTGTC TTTGCATTAT 
TCCTATGATC TCCGCAGTTT GGTGTCTCAG TTATTTAAAA GAAATCCTAG GGATAGACCA 
TCAGTCAACT CCATATTGGA GAAAGGTTTT ATAGCCAAAC GCATTGAAAA GTTTCTCTCT 
CCTCAGCTTA TTGCAGAAGA ATTTTGTCTA AAAACATTTT CGAAGTTTGG ATCACAGCCT 
ATACCAGCTA AAAGACCAGC TTCAGGACAA AACTCGATTT CTGTTATGCC TGCTCAGAAA 
ATTACAAAGC CTGCCGCTAA ATATGGAATA CCTTTAGCAT ATAAGAAATA TGGAGATAAA 
AAATTACACG AAAAGAAACC ACTGCAAAAA CATAAACAGG CCCATCAAAC TCCAGAGAAG 
AGAGTGAATA CTGGAGAAGA AAGGAGGAAA ATATCTGAGG AAGCAGCAAG AAAGAGAAGG 
CTGGAATTTA TTGAAAAAGA AAAGAAACAA AAGGATCAGA TTATTAGTTT AATGAAGGCT 
GAACAAATGA AAAGGCAAGA AAAGGAAAGG TTGGAAAGAA TAAATAGGGC CAGGGAACAA 
GGATGGAGAA ATGTGCTAAG TGCTGGTGGA AGTGGTGAAG TAAAGGCTCC TTTTCTGGGC 
AGTGGAGGGA CTATAGCTCC ATCATCTTTT TCTTCTCGAG GACAGTATGA ACATTACCAT 
GCCATTTTTG ACCAAATGCA GCAACAAAGA GCAGAAGATA ATGAAGCTAA ATGGAAAAGA 
GAAATATATG GTCGAGGTCT TCCAGAAAGA GGAATTCTGC CTGGAGTTCG TCCAGGATTT 
CCTTATGGGG CTGCAGGTCA TCACCATTTT CCTGATGCTG ATGATATTAG AAAAACTTTG 
AAAAGATTGA AGGCGGTGTC TAAACAAGCC AATGCAAACA GGCAAAAAGG GCAGCTAGCT 
GTAGAAAGAG CTAAACAAGT AGAAGAGTTC CTGCAGCGAA AACGGGAAGC TATGCAGAAT 
AAAGCTCGAG CCGAAGGACA TATGGGAATC CTGCAAAACC TGGCAGCTAT GTATGGAGGC 
AGGCCCAGCT CTTCAAGAGG AGGGAAGCCA AGAAACAAAG AGGAAGAGGT TTATCTGGCA 
AGACTGAGGC AAATAAGACT ACAGAATTTC AATGAGCGCC AACAGATTAA AGCCAAACTT 
CGTGGTGAAA AGAAAGAAGC TAATCATTCT GAAGGACAAG AAGGAAGTGA AGAGGCTGAC 
ATGAGGCGCA AAAAAATCGA ATCACTGAAG GCCCATGCAA ATGCACGTGC TGCTGTACTA 
AAAGAACAAC TAGAACGAAA GAGAAAGGAG GCTTATGAGA GAGAAAAAAA AGTGTGGGAA 
GAGCATTTGG TGGCTAAAGG AGTTAAGAGT TCTGATGTTT CTCCACCTTT GGGACAGCAT 
GAAACAGGTG GCTCTCCATC AAAGCAACAG ATGAGATCTG TTATTTCTGT AACTTCAGCT 
TTGAAAGAAG TTGGCGTGGA CAGTAGTTTA ACTGATACCC GGGAAACTTC AGAAGAGATG 
CAAAAGACCA ACAATGCTAT TTCAAGTAAG CGAGAAATAC TTCGTAGATT AAATGAAAAT 
CTTAAAGCTC AAGAAGATGA AAAAGGAAAG CAGAATCTCT CTGATACTTT TGAGATAAAT 
GTTCATGAAG ATGCCAAAGA GCATGAAAAA GAAAAATCAG TTTCATCTGA TCGCAAGAAG 
TGGGAGGCAG GAGGTCAACT TGTGATTCCT CTGGATGAGT TAACACTAGA TACATCCTTC 
TCTACAACTG AAAGACATAC AGTGGGAGAA GTTATTAAAT TAGGTCCTAA TGGATCTCCA 
AGAAGAGCCT GGGGGAAAAG TCCGACAGAT TCTGTTCTAA AGATACTTGG AGAAGCTGAA 
CTACAACTTC AGACAGAACT ATTAGAAAAT ACAACTATTA GAAGTGAGAT TTCTCCCGAA 
GGGGAAAAGT ACAAACCCTT AATTACTGGA GAAAAAAAAG TACAATGTAT TTCACATGAA 
ATAAACCCAT CAGCTATTGT TGATTCTCCT GTTGAGACAA AAAGTCCCGA GTTCAGTGAG 
GCATCTCCAC AGATGTCATT GAAACTGGAA GGAAATTTAG AAGAACCTGA TGATTTGGAA 
ACAGAAATTC TACAAGAGCC AAGTGGAACA AACAAAGATG AGAGCTTGCC ATGCACTATT 
ACTGATGTGT GGATTAGTGA GGAAAAAGAA ACAAAGGAAA CTCAGTCGGC AGATAGGATC 
ACCATTCAGG AAAATGAAGT TTCTGAAGAT GGAGTCTCGA GTACTGTGGA CCAACTTAGT 
GACATTCATA TAGAGCCTGG AACCAATGAT TCTCAGCACT CTAAATGTGA TGTAGATAAG 
TCTGTGCAAC CGGAACCATT TTTCCATAAG GTGGTTCATT CTGAACACTT GAACTTAGTC 
CCTCAAGTTC AATCAGTTCA GTGTTCACCA GAAGAATCCT TTGCATTTCG ATCTCACTCG 
CATTTACCAC CAAAAAATAA AAACAAGAAT TCCTTGCTGA TTGGACTTTC AACTGGTCTG 
TTTGATGCAA ACAACCCAAA GATGTTAAGG ACATGTTCAC TTCCAGATCT CTCAAAGCTG 
TTCAGAACCC TTATGGATGT TCCCACCGTA GGAGATGTTC GTCAAGACAA TCTTGAAATA 
GATGAAATTG AAGATGAAAA CATTAAAGAA GGACCTTCTG ATTCTGAAGA CATTGTGTTT 
GAAGAAACTG ACACAGATTT ACAAGAGCTG CAGGCCTCGA TGGAACAGTT ACTTAGGGAA 
CAACCTGGTG AAGAATACAG TGAAGAAGAA GAGTCAGTCT TGAAGAACAG TGATGTGGAG 
CCAACTGCAA ATGGGACAGA TGTGGCAGAT GAAGATGACA ATCCCAGCAG TGAAAGTGCC 
CTGAACGAAG AATGGCACTC AGATAACAGT GATGGTGAAA TTGCTAGTGA ATGTGAATGC 
GATAGTGTCT TTAACCATTT AGAGGAACTG AGACTTCATC TGGAGCAGGA AATGGGCTTT 
GAAAAATTCT TTGAGGTTTA TGAGAAAATA AAGGCTATTC ATGAAGATGA AGATGAAAAT 
ATTGAAATTT GTTCAAAAAT AGTTCAAAAT ATTTTGGGAA ATGAACATCA GCATCTTTAT 
GCCAAGATTC TTCATTTAGT CATGGCAGAT GGAGCCTACC AAGAAGATAA TGATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863739.1
CDS	1222..4461
Translation	MKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKPLQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERGILPGVRPGFPYGAAGHHHFPDADDIRKTLKRLKAVSKQANANRQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMGILQNLAAMYGGRPSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIKAKLRGEKKEANHSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQNLSDTFEINVHEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLGPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSEISPEGEKYKPLITGEKKVQCISHEINPSAIVDSPVETKSPEFSEASPQMSLKLEGNLEEPDDLETEILQEPSGTNKDESLPCTITDVWISEEKETKETQSADRITIQENEVSEDGVSSTVDQLSDIHIEPGTNDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGEIASECECDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_017008250.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008250.1

ORF Insert Sequence:

ATGAAAAACC TGGTACTGAA GATAATATCT GGATCTTTTC CACCTGTGTC TTTGCATTAT 
TCCTATGATC TCCGCAGTTT GGTGTCTCAG TTATTTAAAA GAAATCCTAG GGATAGACCA 
TCAGTCAACT CCATATTGGA GAAAGGTTTT ATAGCCAAAC GCATTGAAAA GTTTCTCTCT 
CCTCAGCTTA TTGCAGAAGA ATTTTGTCTA AAAACATTTT CGAAGTTTGG ATCACAGCCT 
ATACCAGCTA AAAGACCAGC TTCAGGACAA AACTCGATTT CTGTTATGCC TGCTCAGAAA 
ATTACAAAGC CTGCCGCTAA ATATGGAATA CCTTTAGCAT ATAAGAAATA TGGAGATAAA 
AAATTACACG AAAAGAAACC ACTGCAAAAA CATAAACAGG CCCATCAAAC TCCAGAGAAG 
AGAGTGAATA CTGGAGAAGA AAGGAGGAAA ATATCTGAGG AAGCAGCAAG AAAGAGAAGG 
CTGGAATTTA TTGAAAAAGA AAAGAAACAA AAGGATCAGA TTATTAGTTT AATGAAGGCT 
GAACAAATGA AAAGGCAAGA AAAGGAAAGG TTGGAAAGAA TAAATAGGGC CAGGGAACAA 
GGATGGAGAA ATGTGCTAAG TGCTGGTGGA AGTGGTGAAG TAAAGGCTCC TTTTCTGGGC 
AGTGGAGGGA CTATAGCTCC ATCATCTTTT TCTTCTCGAG GACAGTATGA ACATTACCAT 
GCCATTTTTG ACCAAATGCA GCAACAAAGA GCAGAAGATA ATGAAGCTAA ATGGAAAAGA 
GAAATATATG GTCGAGGTCT TCCAGAAAGA GGAATTCTGC CTGGAGTTCG TCCAGGATTT 
CCTTATGGGG CTGCAGGTCA TCACCATTTT CCTGATGCTG ATGATATTAG AAAAACTTTG 
AAAAGATTGA AGGCGGTGTC TAAACAAGCC AATGCAAACA GGCAAAAAGG GCAGCTAGCT 
GTAGAAAGAG CTAAACAAGT AGAAGAGTTC CTGCAGCGAA AACGGGAAGC TATGCAGAAT 
AAAGCTCGAG CCGAAGGACA TATGGGAATC CTGCAAAACC TGGCAGCTAT GTATGGAGGC 
AGGCCCAGCT CTTCAAGAGG AGGGAAGCCA AGAAACAAAG AGGAAGAGGT TTATCTGGCA 
AGACTGAGGC AAATAAGACT ACAGAATTTC AATGAGCGCC AACAGATTAA AGCCAAACTT 
CGTGGTGAAA AGAAAGAAGC TAATCATTCT GAAGGACAAG AAGGAAGTGA AGAGGCTGAC 
ATGAGGCGCA AAAAAATCGA ATCACTGAAG GCCCATGCAA ATGCACGTGC TGCTGTACTA 
AAAGAACAAC TAGAACGAAA GAGAAAGGAG GCTTATGAGA GAGAAAAAAA AGTGTGGGAA 
GAGCATTTGG TGGCTAAAGG AGTTAAGAGT TCTGATGTTT CTCCACCTTT GGGACAGCAT 
GAAACAGGTG GCTCTCCATC AAAGCAACAG ATGAGATCTG TTATTTCTGT AACTTCAGCT 
TTGAAAGAAG TTGGCGTGGA CAGTAGTTTA ACTGATACCC GGGAAACTTC AGAAGAGATG 
CAAAAGACCA ACAATGCTAT TTCAAGTAAG CGAGAAATAC TTCGTAGATT AAATGAAAAT 
CTTAAAGCTC AAGAAGATGA AAAAGGAAAG CAGAATCTCT CTGATACTTT TGAGATAAAT 
GTTCATGAAG ATGCCAAAGA GCATGAAAAA GAAAAATCAG TTTCATCTGA TCGCAAGAAG 
TGGGAGGCAG GAGGTCAACT TGTGATTCCT CTGGATGAGT TAACACTAGA TACATCCTTC 
TCTACAACTG AAAGACATAC AGTGGGAGAA GTTATTAAAT TAGGTCCTAA TGGATCTCCA 
AGAAGAGCCT GGGGGAAAAG TCCGACAGAT TCTGTTCTAA AGATACTTGG AGAAGCTGAA 
CTACAACTTC AGACAGAACT ATTAGAAAAT ACAACTATTA GAAGTGAGAT TTCTCCCGAA 
GGGGAAAAGT ACAAACCCTT AATTACTGGA GAAAAAAAAG TACAATGTAT TTCACATGAA 
ATAAACCCAT CAGCTATTGT TGATTCTCCT GTTGAGACAA AAAGTCCCGA GTTCAGTGAG 
GCATCTCCAC AGATGTCATT GAAACTGGAA GGAAATTTAG AAGAACCTGA TGATTTGGAA 
ACAGAAATTC TACAAGAGCC AAGTGGAACA AACAAAGATG AGAGCTTGCC ATGCACTATT 
ACTGATGTGT GGATTAGTGA GGAAAAAGAA ACAAAGGAAA CTCAGTCGGC AGATAGGATC 
ACCATTCAGG AAAATGAAGT TTCTGAAGAT GGAGTCTCGA GTACTGTGGA CCAACTTAGT 
GACATTCATA TAGAGCCTGG AACCAATGAT TCTCAGCACT CTAAATGTGA TGTAGATAAG 
TCTGTGCAAC CGGAACCATT TTTCCATAAG GTGGTTCATT CTGAACACTT GAACTTAGTC 
CCTCAAGTTC AATCAGTTCA GTGTTCACCA GAAGAATCCT TTGCATTTCG ATCTCACTCG 
CATTTACCAC CAAAAAATAA AAACAAGAAT TCCTTGCTGA TTGGACTTTC AACTGGTCTG 
TTTGATGCAA ACAACCCAAA GATGTTAAGG ACATGTTCAC TTCCAGATCT CTCAAAGCTG 
TTCAGAACCC TTATGGATGT TCCCACCGTA GGAGATGTTC GTCAAGACAA TCTTGAAATA 
GATGAAATTG AAGATGAAAA CATTAAAGAA GGACCTTCTG ATTCTGAAGA CATTGTGTTT 
GAAGAAACTG ACACAGATTT ACAAGAGCTG CAGGCCTCGA TGGAACAGTT ACTTAGGGAA 
CAACCTGGTG AAGAATACAG TGAAGAAGAA GAGTCAGTCT TGAAGAACAG TGATGTGGAG 
CCAACTGCAA ATGGGACAGA TGTGGCAGAT GAAGATGACA ATCCCAGCAG TGAAAGTGCC 
CTGAACGAAG AATGGCACTC AGATAACAGT GATGGTGAAA TTGCTAGTGA ATGTGAATGC 
GATAGTGTCT TTAACCATTT AGAGGAACTG AGACTTCATC TGGAGCAGGA AATGGGCTTT 
GAAAAATTCT TTGAGGTTTA TGAGAAAATA AAGGCTATTC ATGAAGATGA AGATGAAAAT 
ATTGAAATTT GTTCAAAAAT AGTTCAAAAT ATTTTGGGAA ATGAACATCA GCATCTTTAT 
GCCAAGATTC TTCATTTAGT CATGGCAGAT GGAGCCTACC AAGAAGATAA TGATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_017008249.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863738.1 Homo sapiens NIMA related kinase 1 (NEK1), transcript variant X4, mRNA.

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CloneID	OHu95205
Clone ID Related Accession (Same CDS sequence)	XM_017008250.1 , XM_017008249.1 , XM_024454065.1
Accession Version	XM_017008249.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAACC TGGTACTGAA GATAATATCT GGATCTTTTC CACCTGTGTC TTTGCATTAT 
TCCTATGATC TCCGCAGTTT GGTGTCTCAG TTATTTAAAA GAAATCCTAG GGATAGACCA 
TCAGTCAACT CCATATTGGA GAAAGGTTTT ATAGCCAAAC GCATTGAAAA GTTTCTCTCT 
CCTCAGCTTA TTGCAGAAGA ATTTTGTCTA AAAACATTTT CGAAGTTTGG ATCACAGCCT 
ATACCAGCTA AAAGACCAGC TTCAGGACAA AACTCGATTT CTGTTATGCC TGCTCAGAAA 
ATTACAAAGC CTGCCGCTAA ATATGGAATA CCTTTAGCAT ATAAGAAATA TGGAGATAAA 
AAATTACACG AAAAGAAACC ACTGCAAAAA CATAAACAGG CCCATCAAAC TCCAGAGAAG 
AGAGTGAATA CTGGAGAAGA AAGGAGGAAA ATATCTGAGG AAGCAGCAAG AAAGAGAAGG 
CTGGAATTTA TTGAAAAAGA AAAGAAACAA AAGGATCAGA TTATTAGTTT AATGAAGGCT 
GAACAAATGA AAAGGCAAGA AAAGGAAAGG TTGGAAAGAA TAAATAGGGC CAGGGAACAA 
GGATGGAGAA ATGTGCTAAG TGCTGGTGGA AGTGGTGAAG TAAAGGCTCC TTTTCTGGGC 
AGTGGAGGGA CTATAGCTCC ATCATCTTTT TCTTCTCGAG GACAGTATGA ACATTACCAT 
GCCATTTTTG ACCAAATGCA GCAACAAAGA GCAGAAGATA ATGAAGCTAA ATGGAAAAGA 
GAAATATATG GTCGAGGTCT TCCAGAAAGA GGAATTCTGC CTGGAGTTCG TCCAGGATTT 
CCTTATGGGG CTGCAGGTCA TCACCATTTT CCTGATGCTG ATGATATTAG AAAAACTTTG 
AAAAGATTGA AGGCGGTGTC TAAACAAGCC AATGCAAACA GGCAAAAAGG GCAGCTAGCT 
GTAGAAAGAG CTAAACAAGT AGAAGAGTTC CTGCAGCGAA AACGGGAAGC TATGCAGAAT 
AAAGCTCGAG CCGAAGGACA TATGGGAATC CTGCAAAACC TGGCAGCTAT GTATGGAGGC 
AGGCCCAGCT CTTCAAGAGG AGGGAAGCCA AGAAACAAAG AGGAAGAGGT TTATCTGGCA 
AGACTGAGGC AAATAAGACT ACAGAATTTC AATGAGCGCC AACAGATTAA AGCCAAACTT 
CGTGGTGAAA AGAAAGAAGC TAATCATTCT GAAGGACAAG AAGGAAGTGA AGAGGCTGAC 
ATGAGGCGCA AAAAAATCGA ATCACTGAAG GCCCATGCAA ATGCACGTGC TGCTGTACTA 
AAAGAACAAC TAGAACGAAA GAGAAAGGAG GCTTATGAGA GAGAAAAAAA AGTGTGGGAA 
GAGCATTTGG TGGCTAAAGG AGTTAAGAGT TCTGATGTTT CTCCACCTTT GGGACAGCAT 
GAAACAGGTG GCTCTCCATC AAAGCAACAG ATGAGATCTG TTATTTCTGT AACTTCAGCT 
TTGAAAGAAG TTGGCGTGGA CAGTAGTTTA ACTGATACCC GGGAAACTTC AGAAGAGATG 
CAAAAGACCA ACAATGCTAT TTCAAGTAAG CGAGAAATAC TTCGTAGATT AAATGAAAAT 
CTTAAAGCTC AAGAAGATGA AAAAGGAAAG CAGAATCTCT CTGATACTTT TGAGATAAAT 
GTTCATGAAG ATGCCAAAGA GCATGAAAAA GAAAAATCAG TTTCATCTGA TCGCAAGAAG 
TGGGAGGCAG GAGGTCAACT TGTGATTCCT CTGGATGAGT TAACACTAGA TACATCCTTC 
TCTACAACTG AAAGACATAC AGTGGGAGAA GTTATTAAAT TAGGTCCTAA TGGATCTCCA 
AGAAGAGCCT GGGGGAAAAG TCCGACAGAT TCTGTTCTAA AGATACTTGG AGAAGCTGAA 
CTACAACTTC AGACAGAACT ATTAGAAAAT ACAACTATTA GAAGTGAGAT TTCTCCCGAA 
GGGGAAAAGT ACAAACCCTT AATTACTGGA GAAAAAAAAG TACAATGTAT TTCACATGAA 
ATAAACCCAT CAGCTATTGT TGATTCTCCT GTTGAGACAA AAAGTCCCGA GTTCAGTGAG 
GCATCTCCAC AGATGTCATT GAAACTGGAA GGAAATTTAG AAGAACCTGA TGATTTGGAA 
ACAGAAATTC TACAAGAGCC AAGTGGAACA AACAAAGATG AGAGCTTGCC ATGCACTATT 
ACTGATGTGT GGATTAGTGA GGAAAAAGAA ACAAAGGAAA CTCAGTCGGC AGATAGGATC 
ACCATTCAGG AAAATGAAGT TTCTGAAGAT GGAGTCTCGA GTACTGTGGA CCAACTTAGT 
GACATTCATA TAGAGCCTGG AACCAATGAT TCTCAGCACT CTAAATGTGA TGTAGATAAG 
TCTGTGCAAC CGGAACCATT TTTCCATAAG GTGGTTCATT CTGAACACTT GAACTTAGTC 
CCTCAAGTTC AATCAGTTCA GTGTTCACCA GAAGAATCCT TTGCATTTCG ATCTCACTCG 
CATTTACCAC CAAAAAATAA AAACAAGAAT TCCTTGCTGA TTGGACTTTC AACTGGTCTG 
TTTGATGCAA ACAACCCAAA GATGTTAAGG ACATGTTCAC TTCCAGATCT CTCAAAGCTG 
TTCAGAACCC TTATGGATGT TCCCACCGTA GGAGATGTTC GTCAAGACAA TCTTGAAATA 
GATGAAATTG AAGATGAAAA CATTAAAGAA GGACCTTCTG ATTCTGAAGA CATTGTGTTT 
GAAGAAACTG ACACAGATTT ACAAGAGCTG CAGGCCTCGA TGGAACAGTT ACTTAGGGAA 
CAACCTGGTG AAGAATACAG TGAAGAAGAA GAGTCAGTCT TGAAGAACAG TGATGTGGAG 
CCAACTGCAA ATGGGACAGA TGTGGCAGAT GAAGATGACA ATCCCAGCAG TGAAAGTGCC 
CTGAACGAAG AATGGCACTC AGATAACAGT GATGGTGAAA TTGCTAGTGA ATGTGAATGC 
GATAGTGTCT TTAACCATTT AGAGGAACTG AGACTTCATC TGGAGCAGGA AATGGGCTTT 
GAAAAATTCT TTGAGGTTTA TGAGAAAATA AAGGCTATTC ATGAAGATGA AGATGAAAAT 
ATTGAAATTT GTTCAAAAAT AGTTCAAAAT ATTTTGGGAA ATGAACATCA GCATCTTTAT 
GCCAAGATTC TTCATTTAGT CATGGCAGAT GGAGCCTACC AAGAAGATAA TGATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863738.1
CDS	1104..4343
Translation	MKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKPLQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERGILPGVRPGFPYGAAGHHHFPDADDIRKTLKRLKAVSKQANANRQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMGILQNLAAMYGGRPSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIKAKLRGEKKEANHSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQNLSDTFEINVHEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLGPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSEISPEGEKYKPLITGEKKVQCISHEINPSAIVDSPVETKSPEFSEASPQMSLKLEGNLEEPDDLETEILQEPSGTNKDESLPCTITDVWISEEKETKETQSADRITIQENEVSEDGVSSTVDQLSDIHIEPGTNDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGEIASECECDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_017008249.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008249.1

ORF Insert Sequence:

ATGAAAAACC TGGTACTGAA GATAATATCT GGATCTTTTC CACCTGTGTC TTTGCATTAT 
TCCTATGATC TCCGCAGTTT GGTGTCTCAG TTATTTAAAA GAAATCCTAG GGATAGACCA 
TCAGTCAACT CCATATTGGA GAAAGGTTTT ATAGCCAAAC GCATTGAAAA GTTTCTCTCT 
CCTCAGCTTA TTGCAGAAGA ATTTTGTCTA AAAACATTTT CGAAGTTTGG ATCACAGCCT 
ATACCAGCTA AAAGACCAGC TTCAGGACAA AACTCGATTT CTGTTATGCC TGCTCAGAAA 
ATTACAAAGC CTGCCGCTAA ATATGGAATA CCTTTAGCAT ATAAGAAATA TGGAGATAAA 
AAATTACACG AAAAGAAACC ACTGCAAAAA CATAAACAGG CCCATCAAAC TCCAGAGAAG 
AGAGTGAATA CTGGAGAAGA AAGGAGGAAA ATATCTGAGG AAGCAGCAAG AAAGAGAAGG 
CTGGAATTTA TTGAAAAAGA AAAGAAACAA AAGGATCAGA TTATTAGTTT AATGAAGGCT 
GAACAAATGA AAAGGCAAGA AAAGGAAAGG TTGGAAAGAA TAAATAGGGC CAGGGAACAA 
GGATGGAGAA ATGTGCTAAG TGCTGGTGGA AGTGGTGAAG TAAAGGCTCC TTTTCTGGGC 
AGTGGAGGGA CTATAGCTCC ATCATCTTTT TCTTCTCGAG GACAGTATGA ACATTACCAT 
GCCATTTTTG ACCAAATGCA GCAACAAAGA GCAGAAGATA ATGAAGCTAA ATGGAAAAGA 
GAAATATATG GTCGAGGTCT TCCAGAAAGA GGAATTCTGC CTGGAGTTCG TCCAGGATTT 
CCTTATGGGG CTGCAGGTCA TCACCATTTT CCTGATGCTG ATGATATTAG AAAAACTTTG 
AAAAGATTGA AGGCGGTGTC TAAACAAGCC AATGCAAACA GGCAAAAAGG GCAGCTAGCT 
GTAGAAAGAG CTAAACAAGT AGAAGAGTTC CTGCAGCGAA AACGGGAAGC TATGCAGAAT 
AAAGCTCGAG CCGAAGGACA TATGGGAATC CTGCAAAACC TGGCAGCTAT GTATGGAGGC 
AGGCCCAGCT CTTCAAGAGG AGGGAAGCCA AGAAACAAAG AGGAAGAGGT TTATCTGGCA 
AGACTGAGGC AAATAAGACT ACAGAATTTC AATGAGCGCC AACAGATTAA AGCCAAACTT 
CGTGGTGAAA AGAAAGAAGC TAATCATTCT GAAGGACAAG AAGGAAGTGA AGAGGCTGAC 
ATGAGGCGCA AAAAAATCGA ATCACTGAAG GCCCATGCAA ATGCACGTGC TGCTGTACTA 
AAAGAACAAC TAGAACGAAA GAGAAAGGAG GCTTATGAGA GAGAAAAAAA AGTGTGGGAA 
GAGCATTTGG TGGCTAAAGG AGTTAAGAGT TCTGATGTTT CTCCACCTTT GGGACAGCAT 
GAAACAGGTG GCTCTCCATC AAAGCAACAG ATGAGATCTG TTATTTCTGT AACTTCAGCT 
TTGAAAGAAG TTGGCGTGGA CAGTAGTTTA ACTGATACCC GGGAAACTTC AGAAGAGATG 
CAAAAGACCA ACAATGCTAT TTCAAGTAAG CGAGAAATAC TTCGTAGATT AAATGAAAAT 
CTTAAAGCTC AAGAAGATGA AAAAGGAAAG CAGAATCTCT CTGATACTTT TGAGATAAAT 
GTTCATGAAG ATGCCAAAGA GCATGAAAAA GAAAAATCAG TTTCATCTGA TCGCAAGAAG 
TGGGAGGCAG GAGGTCAACT TGTGATTCCT CTGGATGAGT TAACACTAGA TACATCCTTC 
TCTACAACTG AAAGACATAC AGTGGGAGAA GTTATTAAAT TAGGTCCTAA TGGATCTCCA 
AGAAGAGCCT GGGGGAAAAG TCCGACAGAT TCTGTTCTAA AGATACTTGG AGAAGCTGAA 
CTACAACTTC AGACAGAACT ATTAGAAAAT ACAACTATTA GAAGTGAGAT TTCTCCCGAA 
GGGGAAAAGT ACAAACCCTT AATTACTGGA GAAAAAAAAG TACAATGTAT TTCACATGAA 
ATAAACCCAT CAGCTATTGT TGATTCTCCT GTTGAGACAA AAAGTCCCGA GTTCAGTGAG 
GCATCTCCAC AGATGTCATT GAAACTGGAA GGAAATTTAG AAGAACCTGA TGATTTGGAA 
ACAGAAATTC TACAAGAGCC AAGTGGAACA AACAAAGATG AGAGCTTGCC ATGCACTATT 
ACTGATGTGT GGATTAGTGA GGAAAAAGAA ACAAAGGAAA CTCAGTCGGC AGATAGGATC 
ACCATTCAGG AAAATGAAGT TTCTGAAGAT GGAGTCTCGA GTACTGTGGA CCAACTTAGT 
GACATTCATA TAGAGCCTGG AACCAATGAT TCTCAGCACT CTAAATGTGA TGTAGATAAG 
TCTGTGCAAC CGGAACCATT TTTCCATAAG GTGGTTCATT CTGAACACTT GAACTTAGTC 
CCTCAAGTTC AATCAGTTCA GTGTTCACCA GAAGAATCCT TTGCATTTCG ATCTCACTCG 
CATTTACCAC CAAAAAATAA AAACAAGAAT TCCTTGCTGA TTGGACTTTC AACTGGTCTG 
TTTGATGCAA ACAACCCAAA GATGTTAAGG ACATGTTCAC TTCCAGATCT CTCAAAGCTG 
TTCAGAACCC TTATGGATGT TCCCACCGTA GGAGATGTTC GTCAAGACAA TCTTGAAATA 
GATGAAATTG AAGATGAAAA CATTAAAGAA GGACCTTCTG ATTCTGAAGA CATTGTGTTT 
GAAGAAACTG ACACAGATTT ACAAGAGCTG CAGGCCTCGA TGGAACAGTT ACTTAGGGAA 
CAACCTGGTG AAGAATACAG TGAAGAAGAA GAGTCAGTCT TGAAGAACAG TGATGTGGAG 
CCAACTGCAA ATGGGACAGA TGTGGCAGAT GAAGATGACA ATCCCAGCAG TGAAAGTGCC 
CTGAACGAAG AATGGCACTC AGATAACAGT GATGGTGAAA TTGCTAGTGA ATGTGAATGC 
GATAGTGTCT TTAACCATTT AGAGGAACTG AGACTTCATC TGGAGCAGGA AATGGGCTTT 
GAAAAATTCT TTGAGGTTTA TGAGAAAATA AAGGCTATTC ATGAAGATGA AGATGAAAAT 
ATTGAAATTT GTTCAAAAAT AGTTCAAAAT ATTTTGGGAA ATGAACATCA GCATCTTTAT 
GCCAAGATTC TTCATTTAGT CATGGCAGAT GGAGCCTACC AAGAAGATAA TGATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_024454065.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024309833.1 Homo sapiens NIMA related kinase 1 (NEK1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu95205
Clone ID Related Accession (Same CDS sequence)	XM_017008250.1 , XM_017008249.1 , XM_024454065.1
Accession Version	XM_024454065.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAACC TGGTACTGAA GATAATATCT GGATCTTTTC CACCTGTGTC TTTGCATTAT 
TCCTATGATC TCCGCAGTTT GGTGTCTCAG TTATTTAAAA GAAATCCTAG GGATAGACCA 
TCAGTCAACT CCATATTGGA GAAAGGTTTT ATAGCCAAAC GCATTGAAAA GTTTCTCTCT 
CCTCAGCTTA TTGCAGAAGA ATTTTGTCTA AAAACATTTT CGAAGTTTGG ATCACAGCCT 
ATACCAGCTA AAAGACCAGC TTCAGGACAA AACTCGATTT CTGTTATGCC TGCTCAGAAA 
ATTACAAAGC CTGCCGCTAA ATATGGAATA CCTTTAGCAT ATAAGAAATA TGGAGATAAA 
AAATTACACG AAAAGAAACC ACTGCAAAAA CATAAACAGG CCCATCAAAC TCCAGAGAAG 
AGAGTGAATA CTGGAGAAGA AAGGAGGAAA ATATCTGAGG AAGCAGCAAG AAAGAGAAGG 
CTGGAATTTA TTGAAAAAGA AAAGAAACAA AAGGATCAGA TTATTAGTTT AATGAAGGCT 
GAACAAATGA AAAGGCAAGA AAAGGAAAGG TTGGAAAGAA TAAATAGGGC CAGGGAACAA 
GGATGGAGAA ATGTGCTAAG TGCTGGTGGA AGTGGTGAAG TAAAGGCTCC TTTTCTGGGC 
AGTGGAGGGA CTATAGCTCC ATCATCTTTT TCTTCTCGAG GACAGTATGA ACATTACCAT 
GCCATTTTTG ACCAAATGCA GCAACAAAGA GCAGAAGATA ATGAAGCTAA ATGGAAAAGA 
GAAATATATG GTCGAGGTCT TCCAGAAAGA GGAATTCTGC CTGGAGTTCG TCCAGGATTT 
CCTTATGGGG CTGCAGGTCA TCACCATTTT CCTGATGCTG ATGATATTAG AAAAACTTTG 
AAAAGATTGA AGGCGGTGTC TAAACAAGCC AATGCAAACA GGCAAAAAGG GCAGCTAGCT 
GTAGAAAGAG CTAAACAAGT AGAAGAGTTC CTGCAGCGAA AACGGGAAGC TATGCAGAAT 
AAAGCTCGAG CCGAAGGACA TATGGGAATC CTGCAAAACC TGGCAGCTAT GTATGGAGGC 
AGGCCCAGCT CTTCAAGAGG AGGGAAGCCA AGAAACAAAG AGGAAGAGGT TTATCTGGCA 
AGACTGAGGC AAATAAGACT ACAGAATTTC AATGAGCGCC AACAGATTAA AGCCAAACTT 
CGTGGTGAAA AGAAAGAAGC TAATCATTCT GAAGGACAAG AAGGAAGTGA AGAGGCTGAC 
ATGAGGCGCA AAAAAATCGA ATCACTGAAG GCCCATGCAA ATGCACGTGC TGCTGTACTA 
AAAGAACAAC TAGAACGAAA GAGAAAGGAG GCTTATGAGA GAGAAAAAAA AGTGTGGGAA 
GAGCATTTGG TGGCTAAAGG AGTTAAGAGT TCTGATGTTT CTCCACCTTT GGGACAGCAT 
GAAACAGGTG GCTCTCCATC AAAGCAACAG ATGAGATCTG TTATTTCTGT AACTTCAGCT 
TTGAAAGAAG TTGGCGTGGA CAGTAGTTTA ACTGATACCC GGGAAACTTC AGAAGAGATG 
CAAAAGACCA ACAATGCTAT TTCAAGTAAG CGAGAAATAC TTCGTAGATT AAATGAAAAT 
CTTAAAGCTC AAGAAGATGA AAAAGGAAAG CAGAATCTCT CTGATACTTT TGAGATAAAT 
GTTCATGAAG ATGCCAAAGA GCATGAAAAA GAAAAATCAG TTTCATCTGA TCGCAAGAAG 
TGGGAGGCAG GAGGTCAACT TGTGATTCCT CTGGATGAGT TAACACTAGA TACATCCTTC 
TCTACAACTG AAAGACATAC AGTGGGAGAA GTTATTAAAT TAGGTCCTAA TGGATCTCCA 
AGAAGAGCCT GGGGGAAAAG TCCGACAGAT TCTGTTCTAA AGATACTTGG AGAAGCTGAA 
CTACAACTTC AGACAGAACT ATTAGAAAAT ACAACTATTA GAAGTGAGAT TTCTCCCGAA 
GGGGAAAAGT ACAAACCCTT AATTACTGGA GAAAAAAAAG TACAATGTAT TTCACATGAA 
ATAAACCCAT CAGCTATTGT TGATTCTCCT GTTGAGACAA AAAGTCCCGA GTTCAGTGAG 
GCATCTCCAC AGATGTCATT GAAACTGGAA GGAAATTTAG AAGAACCTGA TGATTTGGAA 
ACAGAAATTC TACAAGAGCC AAGTGGAACA AACAAAGATG AGAGCTTGCC ATGCACTATT 
ACTGATGTGT GGATTAGTGA GGAAAAAGAA ACAAAGGAAA CTCAGTCGGC AGATAGGATC 
ACCATTCAGG AAAATGAAGT TTCTGAAGAT GGAGTCTCGA GTACTGTGGA CCAACTTAGT 
GACATTCATA TAGAGCCTGG AACCAATGAT TCTCAGCACT CTAAATGTGA TGTAGATAAG 
TCTGTGCAAC CGGAACCATT TTTCCATAAG GTGGTTCATT CTGAACACTT GAACTTAGTC 
CCTCAAGTTC AATCAGTTCA GTGTTCACCA GAAGAATCCT TTGCATTTCG ATCTCACTCG 
CATTTACCAC CAAAAAATAA AAACAAGAAT TCCTTGCTGA TTGGACTTTC AACTGGTCTG 
TTTGATGCAA ACAACCCAAA GATGTTAAGG ACATGTTCAC TTCCAGATCT CTCAAAGCTG 
TTCAGAACCC TTATGGATGT TCCCACCGTA GGAGATGTTC GTCAAGACAA TCTTGAAATA 
GATGAAATTG AAGATGAAAA CATTAAAGAA GGACCTTCTG ATTCTGAAGA CATTGTGTTT 
GAAGAAACTG ACACAGATTT ACAAGAGCTG CAGGCCTCGA TGGAACAGTT ACTTAGGGAA 
CAACCTGGTG AAGAATACAG TGAAGAAGAA GAGTCAGTCT TGAAGAACAG TGATGTGGAG 
CCAACTGCAA ATGGGACAGA TGTGGCAGAT GAAGATGACA ATCCCAGCAG TGAAAGTGCC 
CTGAACGAAG AATGGCACTC AGATAACAGT GATGGTGAAA TTGCTAGTGA ATGTGAATGC 
GATAGTGTCT TTAACCATTT AGAGGAACTG AGACTTCATC TGGAGCAGGA AATGGGCTTT 
GAAAAATTCT TTGAGGTTTA TGAGAAAATA AAGGCTATTC ATGAAGATGA AGATGAAAAT 
ATTGAAATTT GTTCAAAAAT AGTTCAAAAT ATTTTGGGAA ATGAACATCA GCATCTTTAT 
GCCAAGATTC TTCATTTAGT CATGGCAGAT GGAGCCTACC AAGAAGATAA TGATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024309833.1
CDS	1147..4386
Translation	MKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKPLQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERGILPGVRPGFPYGAAGHHHFPDADDIRKTLKRLKAVSKQANANRQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMGILQNLAAMYGGRPSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIKAKLRGEKKEANHSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQNLSDTFEINVHEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLGPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSEISPEGEKYKPLITGEKKVQCISHEINPSAIVDSPVETKSPEFSEASPQMSLKLEGNLEEPDDLETEILQEPSGTNKDESLPCTITDVWISEEKETKETQSADRITIQENEVSEDGVSSTVDQLSDIHIEPGTNDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGEIASECECDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_024454065.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024454065.1

ORF Insert Sequence:

ATGAAAAACC TGGTACTGAA GATAATATCT GGATCTTTTC CACCTGTGTC TTTGCATTAT 
TCCTATGATC TCCGCAGTTT GGTGTCTCAG TTATTTAAAA GAAATCCTAG GGATAGACCA 
TCAGTCAACT CCATATTGGA GAAAGGTTTT ATAGCCAAAC GCATTGAAAA GTTTCTCTCT 
CCTCAGCTTA TTGCAGAAGA ATTTTGTCTA AAAACATTTT CGAAGTTTGG ATCACAGCCT 
ATACCAGCTA AAAGACCAGC TTCAGGACAA AACTCGATTT CTGTTATGCC TGCTCAGAAA 
ATTACAAAGC CTGCCGCTAA ATATGGAATA CCTTTAGCAT ATAAGAAATA TGGAGATAAA 
AAATTACACG AAAAGAAACC ACTGCAAAAA CATAAACAGG CCCATCAAAC TCCAGAGAAG 
AGAGTGAATA CTGGAGAAGA AAGGAGGAAA ATATCTGAGG AAGCAGCAAG AAAGAGAAGG 
CTGGAATTTA TTGAAAAAGA AAAGAAACAA AAGGATCAGA TTATTAGTTT AATGAAGGCT 
GAACAAATGA AAAGGCAAGA AAAGGAAAGG TTGGAAAGAA TAAATAGGGC CAGGGAACAA 
GGATGGAGAA ATGTGCTAAG TGCTGGTGGA AGTGGTGAAG TAAAGGCTCC TTTTCTGGGC 
AGTGGAGGGA CTATAGCTCC ATCATCTTTT TCTTCTCGAG GACAGTATGA ACATTACCAT 
GCCATTTTTG ACCAAATGCA GCAACAAAGA GCAGAAGATA ATGAAGCTAA ATGGAAAAGA 
GAAATATATG GTCGAGGTCT TCCAGAAAGA GGAATTCTGC CTGGAGTTCG TCCAGGATTT 
CCTTATGGGG CTGCAGGTCA TCACCATTTT CCTGATGCTG ATGATATTAG AAAAACTTTG 
AAAAGATTGA AGGCGGTGTC TAAACAAGCC AATGCAAACA GGCAAAAAGG GCAGCTAGCT 
GTAGAAAGAG CTAAACAAGT AGAAGAGTTC CTGCAGCGAA AACGGGAAGC TATGCAGAAT 
AAAGCTCGAG CCGAAGGACA TATGGGAATC CTGCAAAACC TGGCAGCTAT GTATGGAGGC 
AGGCCCAGCT CTTCAAGAGG AGGGAAGCCA AGAAACAAAG AGGAAGAGGT TTATCTGGCA 
AGACTGAGGC AAATAAGACT ACAGAATTTC AATGAGCGCC AACAGATTAA AGCCAAACTT 
CGTGGTGAAA AGAAAGAAGC TAATCATTCT GAAGGACAAG AAGGAAGTGA AGAGGCTGAC 
ATGAGGCGCA AAAAAATCGA ATCACTGAAG GCCCATGCAA ATGCACGTGC TGCTGTACTA 
AAAGAACAAC TAGAACGAAA GAGAAAGGAG GCTTATGAGA GAGAAAAAAA AGTGTGGGAA 
GAGCATTTGG TGGCTAAAGG AGTTAAGAGT TCTGATGTTT CTCCACCTTT GGGACAGCAT 
GAAACAGGTG GCTCTCCATC AAAGCAACAG ATGAGATCTG TTATTTCTGT AACTTCAGCT 
TTGAAAGAAG TTGGCGTGGA CAGTAGTTTA ACTGATACCC GGGAAACTTC AGAAGAGATG 
CAAAAGACCA ACAATGCTAT TTCAAGTAAG CGAGAAATAC TTCGTAGATT AAATGAAAAT 
CTTAAAGCTC AAGAAGATGA AAAAGGAAAG CAGAATCTCT CTGATACTTT TGAGATAAAT 
GTTCATGAAG ATGCCAAAGA GCATGAAAAA GAAAAATCAG TTTCATCTGA TCGCAAGAAG 
TGGGAGGCAG GAGGTCAACT TGTGATTCCT CTGGATGAGT TAACACTAGA TACATCCTTC 
TCTACAACTG AAAGACATAC AGTGGGAGAA GTTATTAAAT TAGGTCCTAA TGGATCTCCA 
AGAAGAGCCT GGGGGAAAAG TCCGACAGAT TCTGTTCTAA AGATACTTGG AGAAGCTGAA 
CTACAACTTC AGACAGAACT ATTAGAAAAT ACAACTATTA GAAGTGAGAT TTCTCCCGAA 
GGGGAAAAGT ACAAACCCTT AATTACTGGA GAAAAAAAAG TACAATGTAT TTCACATGAA 
ATAAACCCAT CAGCTATTGT TGATTCTCCT GTTGAGACAA AAAGTCCCGA GTTCAGTGAG 
GCATCTCCAC AGATGTCATT GAAACTGGAA GGAAATTTAG AAGAACCTGA TGATTTGGAA 
ACAGAAATTC TACAAGAGCC AAGTGGAACA AACAAAGATG AGAGCTTGCC ATGCACTATT 
ACTGATGTGT GGATTAGTGA GGAAAAAGAA ACAAAGGAAA CTCAGTCGGC AGATAGGATC 
ACCATTCAGG AAAATGAAGT TTCTGAAGAT GGAGTCTCGA GTACTGTGGA CCAACTTAGT 
GACATTCATA TAGAGCCTGG AACCAATGAT TCTCAGCACT CTAAATGTGA TGTAGATAAG 
TCTGTGCAAC CGGAACCATT TTTCCATAAG GTGGTTCATT CTGAACACTT GAACTTAGTC 
CCTCAAGTTC AATCAGTTCA GTGTTCACCA GAAGAATCCT TTGCATTTCG ATCTCACTCG 
CATTTACCAC CAAAAAATAA AAACAAGAAT TCCTTGCTGA TTGGACTTTC AACTGGTCTG 
TTTGATGCAA ACAACCCAAA GATGTTAAGG ACATGTTCAC TTCCAGATCT CTCAAAGCTG 
TTCAGAACCC TTATGGATGT TCCCACCGTA GGAGATGTTC GTCAAGACAA TCTTGAAATA 
GATGAAATTG AAGATGAAAA CATTAAAGAA GGACCTTCTG ATTCTGAAGA CATTGTGTTT 
GAAGAAACTG ACACAGATTT ACAAGAGCTG CAGGCCTCGA TGGAACAGTT ACTTAGGGAA 
CAACCTGGTG AAGAATACAG TGAAGAAGAA GAGTCAGTCT TGAAGAACAG TGATGTGGAG 
CCAACTGCAA ATGGGACAGA TGTGGCAGAT GAAGATGACA ATCCCAGCAG TGAAAGTGCC 
CTGAACGAAG AATGGCACTC AGATAACAGT GATGGTGAAA TTGCTAGTGA ATGTGAATGC 
GATAGTGTCT TTAACCATTT AGAGGAACTG AGACTTCATC TGGAGCAGGA AATGGGCTTT 
GAAAAATTCT TTGAGGTTTA TGAGAAAATA AAGGCTATTC ATGAAGATGA AGATGAAAAT 
ATTGAAATTT GTTCAAAAAT AGTTCAAAAT ATTTTGGGAA ATGAACATCA GCATCTTTAT 
GCCAAGATTC TTCATTTAGT CATGGCAGAT GGAGCCTACC AAGAAGATAA TGATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_017008252.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863741.1 Homo sapiens NIMA related kinase 1 (NEK1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu95206
Clone ID Related Accession (Same CDS sequence)	XM_017008252.2 , XM_017008251.1 , XM_017008252.1
Accession Version	XM_017008252.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3156bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017008252.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAACC TGGTACTGAA GATAATATCT GGATCTTTTC CACCTGTGTC TTTGCATTAT 
TCCTATGATC TCCGCAGTTT GGTGTCTCAG TTATTTAAAA GAAATCCTAG GGATAGACCA 
TCAGTCAACT CCATATTGGA GAAAGGTTTT ATAGCCAAAC GCATTGAAAA GTTTCTCTCT 
CCTCAGCTTA TTGCAGAAGA ATTTTGTCTA AAAACATTTT CGAAGTTTGG ATCACAGCCT 
ATACCAGCTA AAAGACCAGC TTCAGGACAA AACTCGATTT CTGTTATGCC TGCTCAGAAA 
ATTACAAAGC CTGCCGCTAA ATATGGAATA CCTTTAGCAT ATAAGAAATA TGGAGATAAA 
AAATTACACG AAAAGAAACC ACTGCAAAAA CATAAACAGG CCCATCAAAC TCCAGAGAAG 
AGAGTGAATA CTGGAGAAGA AAGGAGGAAA ATATCTGAGG AAGCAGCAAG AAAGAGAAGG 
CTGGAATTTA TTGAAAAAGA AAAGAAACAA AAGGATCAGA TTATTAGTTT AATGAAGGCT 
GAACAAATGA AAAGGCAAGA AAAGGAAAGG TTGGAAAGAA TAAATAGGGC CAGGGAACAA 
GGATGGAGAA ATGTGCTAAG TGCTGGTGGA AGTGGTGAAG TAAAGGCTCC TTTTCTGGGC 
AGTGGAGGGA CTATAGCTCC ATCATCTTTT TCTTCTCGAG GACAGTATGA ACATTACCAT 
GCCATTTTTG ACCAAATGCA GCAACAAAGA GCAGAAGATA ATGAAGCTAA ATGGAAAAGA 
GAAATATATG GTCGAGGTCT TCCAGAAAGA GGAATTCTGC CTGGAGTTCG TCCAGGATTT 
CCTTATGGGG CTGCAGGTCA TCACCATTTT CCTGATGCTG ATGATATTAG AAAAACTTTG 
AAAAGATTGA AGGCGGTGTC TAAACAAGCC AATGCAAACA GGCAAAAAGG GCAGCTAGCT 
GTAGAAAGAG CTAAACAAGT AGAAGAGTTC CTGCAGCGAA AACGGGAAGC TATGCAGAAT 
AAAGCTCGAG CCGAAGGACA TATGGTTTAT CTGGCAAGAC TGAGGCAAAT AAGACTACAG 
AATTTCAATG AGCGCCAACA GATTAAAGCC AAACTTCGTG GTGAAAAGAA AGAAGCTAAT 
CATTCTGAAG GACAAGAAGG AAGTGAAGAG GCTGACATGA GGCGCAAAAA AATCGAATCA 
CTGAAGGCCC ATGCAAATGC ACGTGCTGCT GTACTAAAAG AACAACTAGA ACGAAAGAGA 
AAGGAGGCTT ATGAGAGAGA AAAAAAAGTG TGGGAAGAGC ATTTGGTGGC TAAAGGAGTT 
AAGAGTTCTG ATGTTTCTCC ACCTTTGGGA CAGCATGAAA CAGGTGGCTC TCCATCAAAG 
CAACAGATGA GATCTGTTAT TTCTGTAACT TCAGCTTTGA AAGAAGTTGG CGTGGACAGT 
AGTTTAACTG ATACCCGGGA AACTTCAGAA GAGATGCAAA AGACCAACAA TGCTATTTCA 
AGTAAGCGAG AAATACTTCG TAGATTAAAT GAAAATCTTA AAGCTCAAGA AGATGAAAAA 
GGAAAGCAGA ATCTCTCTGA TACTTTTGAG ATAAATGTTC ATGAAGATGC CAAAGAGCAT 
GAAAAAGAAA AATCAGTTTC ATCTGATCGC AAGAAGTGGG AGGCAGGAGG TCAACTTGTG 
ATTCCTCTGG ATGAGTTAAC ACTAGATACA TCCTTCTCTA CAACTGAAAG ACATACAGTG 
GGAGAAGTTA TTAAATTAGG TCCTAATGGA TCTCCAAGAA GAGCCTGGGG GAAAAGTCCG 
ACAGATTCTG TTCTAAAGAT ACTTGGAGAA GCTGAACTAC AACTTCAGAC AGAACTATTA 
GAAAATACAA CTATTAGAAG TGAGATTTCT CCCGAAGGGG AAAAGTACAA ACCCTTAATT 
ACTGGAGAAA AAAAAGTACA ATGTATTTCA CATGAAATAA ACCCATCAGC TATTGTTGAT 
TCTCCTGTTG AGACAAAAAG TCCCGAGTTC AGTGAGGCAT CTCCACAGAT GTCATTGAAA 
CTGGAAGGAA ATTTAGAAGA ACCTGATGAT TTGGAAACAG AAATTCTACA AGAGCCAAGT 
GGAACAAACA AAGATGAGAG CTTGCCATGC ACTATTACTG ATGTGTGGAT TAGTGAGGAA 
AAAGAAACAA AGGAAACTCA GTCGGCAGAT AGGATCACCA TTCAGGAAAA TGAAGTTTCT 
GAAGATGGAG TCTCGAGTAC TGTGGACCAA CTTAGTGACA TTCATATAGA GCCTGGAACC 
AATGATTCTC AGCACTCTAA ATGTGATGTA GATAAGTCTG TGCAACCGGA ACCATTTTTC 
CATAAGGTGG TTCATTCTGA ACACTTGAAC TTAGTCCCTC AAGTTCAATC AGTTCAGTGT 
TCACCAGAAG AATCCTTTGC ATTTCGATCT CACTCGCATT TACCACCAAA AAATAAAAAC 
AAGAATTCCT TGCTGATTGG ACTTTCAACT GGTCTGTTTG ATGCAAACAA CCCAAAGATG 
TTAAGGACAT GTTCACTTCC AGATCTCTCA AAGCTGTTCA GAACCCTTAT GGATGTTCCC 
ACCGTAGGAG ATGTTCGTCA AGACAATCTT GAAATAGATG AAATTGAAGA TGAAAACATT 
AAAGAAGGAC CTTCTGATTC TGAAGACATT GTGTTTGAAG AAACTGACAC AGATTTACAA 
GAGCTGCAGG CCTCGATGGA ACAGTTACTT AGGGAACAAC CTGGTGAAGA ATACAGTGAA 
GAAGAAGAGT CAGTCTTGAA GAACAGTGAT GTGGAGCCAA CTGCAAATGG GACAGATGTG 
GCAGATGAAG ATGACAATCC CAGCAGTGAA AGTGCCCTGA ACGAAGAATG GCACTCAGAT 
AACAGTGATG GTGAAATTGC TAGTGAATGT GAATGCGATA GTGTCTTTAA CCATTTAGAG 
GAACTGAGAC TTCATCTGGA GCAGGAAATG GGCTTTGAAA AATTCTTTGA GGTTTATGAG 
AAAATAAAGG CTATTCATGA AGATGAAGAT GAAAATATTG AAATTTGTTC AAAAATAGTT 
CAAAATATTT TGGGAAATGA ACATCAGCAT CTTTATGCCA AGATTCTTCA TTTAGTCATG 
GCAGATGGAG CCTACCAAGA AGATAATGAT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863741.1
CDS	994..4149
Translation	MKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKPLQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERGILPGVRPGFPYGAAGHHHFPDADDIRKTLKRLKAVSKQANANRQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMVYLARLRQIRLQNFNERQQIKAKLRGEKKEANHSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQNLSDTFEINVHEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLGPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSEISPEGEKYKPLITGEKKVQCISHEINPSAIVDSPVETKSPEFSEASPQMSLKLEGNLEEPDDLETEILQEPSGTNKDESLPCTITDVWISEEKETKETQSADRITIQENEVSEDGVSSTVDQLSDIHIEPGTNDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGEIASECECDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_017008252.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008252.2

ORF Insert Sequence:

ATGAAAAACC TGGTACTGAA GATAATATCT GGATCTTTTC CACCTGTGTC TTTGCATTAT 
TCCTATGATC TCCGCAGTTT GGTGTCTCAG TTATTTAAAA GAAATCCTAG GGATAGACCA 
TCAGTCAACT CCATATTGGA GAAAGGTTTT ATAGCCAAAC GCATTGAAAA GTTTCTCTCT 
CCTCAGCTTA TTGCAGAAGA ATTTTGTCTA AAAACATTTT CGAAGTTTGG ATCACAGCCT 
ATACCAGCTA AAAGACCAGC TTCAGGACAA AACTCGATTT CTGTTATGCC TGCTCAGAAA 
ATTACAAAGC CTGCCGCTAA ATATGGAATA CCTTTAGCAT ATAAGAAATA TGGAGATAAA 
AAATTACACG AAAAGAAACC ACTGCAAAAA CATAAACAGG CCCATCAAAC TCCAGAGAAG 
AGAGTGAATA CTGGAGAAGA AAGGAGGAAA ATATCTGAGG AAGCAGCAAG AAAGAGAAGG 
CTGGAATTTA TTGAAAAAGA AAAGAAACAA AAGGATCAGA TTATTAGTTT AATGAAGGCT 
GAACAAATGA AAAGGCAAGA AAAGGAAAGG TTGGAAAGAA TAAATAGGGC CAGGGAACAA 
GGATGGAGAA ATGTGCTAAG TGCTGGTGGA AGTGGTGAAG TAAAGGCTCC TTTTCTGGGC 
AGTGGAGGGA CTATAGCTCC ATCATCTTTT TCTTCTCGAG GACAGTATGA ACATTACCAT 
GCCATTTTTG ACCAAATGCA GCAACAAAGA GCAGAAGATA ATGAAGCTAA ATGGAAAAGA 
GAAATATATG GTCGAGGTCT TCCAGAAAGA GGAATTCTGC CTGGAGTTCG TCCAGGATTT 
CCTTATGGGG CTGCAGGTCA TCACCATTTT CCTGATGCTG ATGATATTAG AAAAACTTTG 
AAAAGATTGA AGGCGGTGTC TAAACAAGCC AATGCAAACA GGCAAAAAGG GCAGCTAGCT 
GTAGAAAGAG CTAAACAAGT AGAAGAGTTC CTGCAGCGAA AACGGGAAGC TATGCAGAAT 
AAAGCTCGAG CCGAAGGACA TATGGTTTAT CTGGCAAGAC TGAGGCAAAT AAGACTACAG 
AATTTCAATG AGCGCCAACA GATTAAAGCC AAACTTCGTG GTGAAAAGAA AGAAGCTAAT 
CATTCTGAAG GACAAGAAGG AAGTGAAGAG GCTGACATGA GGCGCAAAAA AATCGAATCA 
CTGAAGGCCC ATGCAAATGC ACGTGCTGCT GTACTAAAAG AACAACTAGA ACGAAAGAGA 
AAGGAGGCTT ATGAGAGAGA AAAAAAAGTG TGGGAAGAGC ATTTGGTGGC TAAAGGAGTT 
AAGAGTTCTG ATGTTTCTCC ACCTTTGGGA CAGCATGAAA CAGGTGGCTC TCCATCAAAG 
CAACAGATGA GATCTGTTAT TTCTGTAACT TCAGCTTTGA AAGAAGTTGG CGTGGACAGT 
AGTTTAACTG ATACCCGGGA AACTTCAGAA GAGATGCAAA AGACCAACAA TGCTATTTCA 
AGTAAGCGAG AAATACTTCG TAGATTAAAT GAAAATCTTA AAGCTCAAGA AGATGAAAAA 
GGAAAGCAGA ATCTCTCTGA TACTTTTGAG ATAAATGTTC ATGAAGATGC CAAAGAGCAT 
GAAAAAGAAA AATCAGTTTC ATCTGATCGC AAGAAGTGGG AGGCAGGAGG TCAACTTGTG 
ATTCCTCTGG ATGAGTTAAC ACTAGATACA TCCTTCTCTA CAACTGAAAG ACATACAGTG 
GGAGAAGTTA TTAAATTAGG TCCTAATGGA TCTCCAAGAA GAGCCTGGGG GAAAAGTCCG 
ACAGATTCTG TTCTAAAGAT ACTTGGAGAA GCTGAACTAC AACTTCAGAC AGAACTATTA 
GAAAATACAA CTATTAGAAG TGAGATTTCT CCCGAAGGGG AAAAGTACAA ACCCTTAATT 
ACTGGAGAAA AAAAAGTACA ATGTATTTCA CATGAAATAA ACCCATCAGC TATTGTTGAT 
TCTCCTGTTG AGACAAAAAG TCCCGAGTTC AGTGAGGCAT CTCCACAGAT GTCATTGAAA 
CTGGAAGGAA ATTTAGAAGA ACCTGATGAT TTGGAAACAG AAATTCTACA AGAGCCAAGT 
GGAACAAACA AAGATGAGAG CTTGCCATGC ACTATTACTG ATGTGTGGAT TAGTGAGGAA 
AAAGAAACAA AGGAAACTCA GTCGGCAGAT AGGATCACCA TTCAGGAAAA TGAAGTTTCT 
GAAGATGGAG TCTCGAGTAC TGTGGACCAA CTTAGTGACA TTCATATAGA GCCTGGAACC 
AATGATTCTC AGCACTCTAA ATGTGATGTA GATAAGTCTG TGCAACCGGA ACCATTTTTC 
CATAAGGTGG TTCATTCTGA ACACTTGAAC TTAGTCCCTC AAGTTCAATC AGTTCAGTGT 
TCACCAGAAG AATCCTTTGC ATTTCGATCT CACTCGCATT TACCACCAAA AAATAAAAAC 
AAGAATTCCT TGCTGATTGG ACTTTCAACT GGTCTGTTTG ATGCAAACAA CCCAAAGATG 
TTAAGGACAT GTTCACTTCC AGATCTCTCA AAGCTGTTCA GAACCCTTAT GGATGTTCCC 
ACCGTAGGAG ATGTTCGTCA AGACAATCTT GAAATAGATG AAATTGAAGA TGAAAACATT 
AAAGAAGGAC CTTCTGATTC TGAAGACATT GTGTTTGAAG AAACTGACAC AGATTTACAA 
GAGCTGCAGG CCTCGATGGA ACAGTTACTT AGGGAACAAC CTGGTGAAGA ATACAGTGAA 
GAAGAAGAGT CAGTCTTGAA GAACAGTGAT GTGGAGCCAA CTGCAAATGG GACAGATGTG 
GCAGATGAAG ATGACAATCC CAGCAGTGAA AGTGCCCTGA ACGAAGAATG GCACTCAGAT 
AACAGTGATG GTGAAATTGC TAGTGAATGT GAATGCGATA GTGTCTTTAA CCATTTAGAG 
GAACTGAGAC TTCATCTGGA GCAGGAAATG GGCTTTGAAA AATTCTTTGA GGTTTATGAG 
AAAATAAAGG CTATTCATGA AGATGAAGAT GAAAATATTG AAATTTGTTC AAAAATAGTT 
CAAAATATTT TGGGAAATGA ACATCAGCAT CTTTATGCCA AGATTCTTCA TTTAGTCATG 
GCAGATGGAG CCTACCAAGA AGATAATGAT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_017008251.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863740.1 Homo sapiens NIMA related kinase 1 (NEK1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu95206
Clone ID Related Accession (Same CDS sequence)	XM_017008252.2 , XM_017008251.1 , XM_017008252.1
Accession Version	XM_017008251.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3156bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAACC TGGTACTGAA GATAATATCT GGATCTTTTC CACCTGTGTC TTTGCATTAT 
TCCTATGATC TCCGCAGTTT GGTGTCTCAG TTATTTAAAA GAAATCCTAG GGATAGACCA 
TCAGTCAACT CCATATTGGA GAAAGGTTTT ATAGCCAAAC GCATTGAAAA GTTTCTCTCT 
CCTCAGCTTA TTGCAGAAGA ATTTTGTCTA AAAACATTTT CGAAGTTTGG ATCACAGCCT 
ATACCAGCTA AAAGACCAGC TTCAGGACAA AACTCGATTT CTGTTATGCC TGCTCAGAAA 
ATTACAAAGC CTGCCGCTAA ATATGGAATA CCTTTAGCAT ATAAGAAATA TGGAGATAAA 
AAATTACACG AAAAGAAACC ACTGCAAAAA CATAAACAGG CCCATCAAAC TCCAGAGAAG 
AGAGTGAATA CTGGAGAAGA AAGGAGGAAA ATATCTGAGG AAGCAGCAAG AAAGAGAAGG 
CTGGAATTTA TTGAAAAAGA AAAGAAACAA AAGGATCAGA TTATTAGTTT AATGAAGGCT 
GAACAAATGA AAAGGCAAGA AAAGGAAAGG TTGGAAAGAA TAAATAGGGC CAGGGAACAA 
GGATGGAGAA ATGTGCTAAG TGCTGGTGGA AGTGGTGAAG TAAAGGCTCC TTTTCTGGGC 
AGTGGAGGGA CTATAGCTCC ATCATCTTTT TCTTCTCGAG GACAGTATGA ACATTACCAT 
GCCATTTTTG ACCAAATGCA GCAACAAAGA GCAGAAGATA ATGAAGCTAA ATGGAAAAGA 
GAAATATATG GTCGAGGTCT TCCAGAAAGA GGAATTCTGC CTGGAGTTCG TCCAGGATTT 
CCTTATGGGG CTGCAGGTCA TCACCATTTT CCTGATGCTG ATGATATTAG AAAAACTTTG 
AAAAGATTGA AGGCGGTGTC TAAACAAGCC AATGCAAACA GGCAAAAAGG GCAGCTAGCT 
GTAGAAAGAG CTAAACAAGT AGAAGAGTTC CTGCAGCGAA AACGGGAAGC TATGCAGAAT 
AAAGCTCGAG CCGAAGGACA TATGGTTTAT CTGGCAAGAC TGAGGCAAAT AAGACTACAG 
AATTTCAATG AGCGCCAACA GATTAAAGCC AAACTTCGTG GTGAAAAGAA AGAAGCTAAT 
CATTCTGAAG GACAAGAAGG AAGTGAAGAG GCTGACATGA GGCGCAAAAA AATCGAATCA 
CTGAAGGCCC ATGCAAATGC ACGTGCTGCT GTACTAAAAG AACAACTAGA ACGAAAGAGA 
AAGGAGGCTT ATGAGAGAGA AAAAAAAGTG TGGGAAGAGC ATTTGGTGGC TAAAGGAGTT 
AAGAGTTCTG ATGTTTCTCC ACCTTTGGGA CAGCATGAAA CAGGTGGCTC TCCATCAAAG 
CAACAGATGA GATCTGTTAT TTCTGTAACT TCAGCTTTGA AAGAAGTTGG CGTGGACAGT 
AGTTTAACTG ATACCCGGGA AACTTCAGAA GAGATGCAAA AGACCAACAA TGCTATTTCA 
AGTAAGCGAG AAATACTTCG TAGATTAAAT GAAAATCTTA AAGCTCAAGA AGATGAAAAA 
GGAAAGCAGA ATCTCTCTGA TACTTTTGAG ATAAATGTTC ATGAAGATGC CAAAGAGCAT 
GAAAAAGAAA AATCAGTTTC ATCTGATCGC AAGAAGTGGG AGGCAGGAGG TCAACTTGTG 
ATTCCTCTGG ATGAGTTAAC ACTAGATACA TCCTTCTCTA CAACTGAAAG ACATACAGTG 
GGAGAAGTTA TTAAATTAGG TCCTAATGGA TCTCCAAGAA GAGCCTGGGG GAAAAGTCCG 
ACAGATTCTG TTCTAAAGAT ACTTGGAGAA GCTGAACTAC AACTTCAGAC AGAACTATTA 
GAAAATACAA CTATTAGAAG TGAGATTTCT CCCGAAGGGG AAAAGTACAA ACCCTTAATT 
ACTGGAGAAA AAAAAGTACA ATGTATTTCA CATGAAATAA ACCCATCAGC TATTGTTGAT 
TCTCCTGTTG AGACAAAAAG TCCCGAGTTC AGTGAGGCAT CTCCACAGAT GTCATTGAAA 
CTGGAAGGAA ATTTAGAAGA ACCTGATGAT TTGGAAACAG AAATTCTACA AGAGCCAAGT 
GGAACAAACA AAGATGAGAG CTTGCCATGC ACTATTACTG ATGTGTGGAT TAGTGAGGAA 
AAAGAAACAA AGGAAACTCA GTCGGCAGAT AGGATCACCA TTCAGGAAAA TGAAGTTTCT 
GAAGATGGAG TCTCGAGTAC TGTGGACCAA CTTAGTGACA TTCATATAGA GCCTGGAACC 
AATGATTCTC AGCACTCTAA ATGTGATGTA GATAAGTCTG TGCAACCGGA ACCATTTTTC 
CATAAGGTGG TTCATTCTGA ACACTTGAAC TTAGTCCCTC AAGTTCAATC AGTTCAGTGT 
TCACCAGAAG AATCCTTTGC ATTTCGATCT CACTCGCATT TACCACCAAA AAATAAAAAC 
AAGAATTCCT TGCTGATTGG ACTTTCAACT GGTCTGTTTG ATGCAAACAA CCCAAAGATG 
TTAAGGACAT GTTCACTTCC AGATCTCTCA AAGCTGTTCA GAACCCTTAT GGATGTTCCC 
ACCGTAGGAG ATGTTCGTCA AGACAATCTT GAAATAGATG AAATTGAAGA TGAAAACATT 
AAAGAAGGAC CTTCTGATTC TGAAGACATT GTGTTTGAAG AAACTGACAC AGATTTACAA 
GAGCTGCAGG CCTCGATGGA ACAGTTACTT AGGGAACAAC CTGGTGAAGA ATACAGTGAA 
GAAGAAGAGT CAGTCTTGAA GAACAGTGAT GTGGAGCCAA CTGCAAATGG GACAGATGTG 
GCAGATGAAG ATGACAATCC CAGCAGTGAA AGTGCCCTGA ACGAAGAATG GCACTCAGAT 
AACAGTGATG GTGAAATTGC TAGTGAATGT GAATGCGATA GTGTCTTTAA CCATTTAGAG 
GAACTGAGAC TTCATCTGGA GCAGGAAATG GGCTTTGAAA AATTCTTTGA GGTTTATGAG 
AAAATAAAGG CTATTCATGA AGATGAAGAT GAAAATATTG AAATTTGTTC AAAAATAGTT 
CAAAATATTT TGGGAAATGA ACATCAGCAT CTTTATGCCA AGATTCTTCA TTTAGTCATG 
GCAGATGGAG CCTACCAAGA AGATAATGAT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863740.1
CDS	1104..4259
Translation	MKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKPLQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERGILPGVRPGFPYGAAGHHHFPDADDIRKTLKRLKAVSKQANANRQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMVYLARLRQIRLQNFNERQQIKAKLRGEKKEANHSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQNLSDTFEINVHEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLGPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSEISPEGEKYKPLITGEKKVQCISHEINPSAIVDSPVETKSPEFSEASPQMSLKLEGNLEEPDDLETEILQEPSGTNKDESLPCTITDVWISEEKETKETQSADRITIQENEVSEDGVSSTVDQLSDIHIEPGTNDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGEIASECECDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_017008251.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008251.1

ORF Insert Sequence:

ATGAAAAACC TGGTACTGAA GATAATATCT GGATCTTTTC CACCTGTGTC TTTGCATTAT 
TCCTATGATC TCCGCAGTTT GGTGTCTCAG TTATTTAAAA GAAATCCTAG GGATAGACCA 
TCAGTCAACT CCATATTGGA GAAAGGTTTT ATAGCCAAAC GCATTGAAAA GTTTCTCTCT 
CCTCAGCTTA TTGCAGAAGA ATTTTGTCTA AAAACATTTT CGAAGTTTGG ATCACAGCCT 
ATACCAGCTA AAAGACCAGC TTCAGGACAA AACTCGATTT CTGTTATGCC TGCTCAGAAA 
ATTACAAAGC CTGCCGCTAA ATATGGAATA CCTTTAGCAT ATAAGAAATA TGGAGATAAA 
AAATTACACG AAAAGAAACC ACTGCAAAAA CATAAACAGG CCCATCAAAC TCCAGAGAAG 
AGAGTGAATA CTGGAGAAGA AAGGAGGAAA ATATCTGAGG AAGCAGCAAG AAAGAGAAGG 
CTGGAATTTA TTGAAAAAGA AAAGAAACAA AAGGATCAGA TTATTAGTTT AATGAAGGCT 
GAACAAATGA AAAGGCAAGA AAAGGAAAGG TTGGAAAGAA TAAATAGGGC CAGGGAACAA 
GGATGGAGAA ATGTGCTAAG TGCTGGTGGA AGTGGTGAAG TAAAGGCTCC TTTTCTGGGC 
AGTGGAGGGA CTATAGCTCC ATCATCTTTT TCTTCTCGAG GACAGTATGA ACATTACCAT 
GCCATTTTTG ACCAAATGCA GCAACAAAGA GCAGAAGATA ATGAAGCTAA ATGGAAAAGA 
GAAATATATG GTCGAGGTCT TCCAGAAAGA GGAATTCTGC CTGGAGTTCG TCCAGGATTT 
CCTTATGGGG CTGCAGGTCA TCACCATTTT CCTGATGCTG ATGATATTAG AAAAACTTTG 
AAAAGATTGA AGGCGGTGTC TAAACAAGCC AATGCAAACA GGCAAAAAGG GCAGCTAGCT 
GTAGAAAGAG CTAAACAAGT AGAAGAGTTC CTGCAGCGAA AACGGGAAGC TATGCAGAAT 
AAAGCTCGAG CCGAAGGACA TATGGTTTAT CTGGCAAGAC TGAGGCAAAT AAGACTACAG 
AATTTCAATG AGCGCCAACA GATTAAAGCC AAACTTCGTG GTGAAAAGAA AGAAGCTAAT 
CATTCTGAAG GACAAGAAGG AAGTGAAGAG GCTGACATGA GGCGCAAAAA AATCGAATCA 
CTGAAGGCCC ATGCAAATGC ACGTGCTGCT GTACTAAAAG AACAACTAGA ACGAAAGAGA 
AAGGAGGCTT ATGAGAGAGA AAAAAAAGTG TGGGAAGAGC ATTTGGTGGC TAAAGGAGTT 
AAGAGTTCTG ATGTTTCTCC ACCTTTGGGA CAGCATGAAA CAGGTGGCTC TCCATCAAAG 
CAACAGATGA GATCTGTTAT TTCTGTAACT TCAGCTTTGA AAGAAGTTGG CGTGGACAGT 
AGTTTAACTG ATACCCGGGA AACTTCAGAA GAGATGCAAA AGACCAACAA TGCTATTTCA 
AGTAAGCGAG AAATACTTCG TAGATTAAAT GAAAATCTTA AAGCTCAAGA AGATGAAAAA 
GGAAAGCAGA ATCTCTCTGA TACTTTTGAG ATAAATGTTC ATGAAGATGC CAAAGAGCAT 
GAAAAAGAAA AATCAGTTTC ATCTGATCGC AAGAAGTGGG AGGCAGGAGG TCAACTTGTG 
ATTCCTCTGG ATGAGTTAAC ACTAGATACA TCCTTCTCTA CAACTGAAAG ACATACAGTG 
GGAGAAGTTA TTAAATTAGG TCCTAATGGA TCTCCAAGAA GAGCCTGGGG GAAAAGTCCG 
ACAGATTCTG TTCTAAAGAT ACTTGGAGAA GCTGAACTAC AACTTCAGAC AGAACTATTA 
GAAAATACAA CTATTAGAAG TGAGATTTCT CCCGAAGGGG AAAAGTACAA ACCCTTAATT 
ACTGGAGAAA AAAAAGTACA ATGTATTTCA CATGAAATAA ACCCATCAGC TATTGTTGAT 
TCTCCTGTTG AGACAAAAAG TCCCGAGTTC AGTGAGGCAT CTCCACAGAT GTCATTGAAA 
CTGGAAGGAA ATTTAGAAGA ACCTGATGAT TTGGAAACAG AAATTCTACA AGAGCCAAGT 
GGAACAAACA AAGATGAGAG CTTGCCATGC ACTATTACTG ATGTGTGGAT TAGTGAGGAA 
AAAGAAACAA AGGAAACTCA GTCGGCAGAT AGGATCACCA TTCAGGAAAA TGAAGTTTCT 
GAAGATGGAG TCTCGAGTAC TGTGGACCAA CTTAGTGACA TTCATATAGA GCCTGGAACC 
AATGATTCTC AGCACTCTAA ATGTGATGTA GATAAGTCTG TGCAACCGGA ACCATTTTTC 
CATAAGGTGG TTCATTCTGA ACACTTGAAC TTAGTCCCTC AAGTTCAATC AGTTCAGTGT 
TCACCAGAAG AATCCTTTGC ATTTCGATCT CACTCGCATT TACCACCAAA AAATAAAAAC 
AAGAATTCCT TGCTGATTGG ACTTTCAACT GGTCTGTTTG ATGCAAACAA CCCAAAGATG 
TTAAGGACAT GTTCACTTCC AGATCTCTCA AAGCTGTTCA GAACCCTTAT GGATGTTCCC 
ACCGTAGGAG ATGTTCGTCA AGACAATCTT GAAATAGATG AAATTGAAGA TGAAAACATT 
AAAGAAGGAC CTTCTGATTC TGAAGACATT GTGTTTGAAG AAACTGACAC AGATTTACAA 
GAGCTGCAGG CCTCGATGGA ACAGTTACTT AGGGAACAAC CTGGTGAAGA ATACAGTGAA 
GAAGAAGAGT CAGTCTTGAA GAACAGTGAT GTGGAGCCAA CTGCAAATGG GACAGATGTG 
GCAGATGAAG ATGACAATCC CAGCAGTGAA AGTGCCCTGA ACGAAGAATG GCACTCAGAT 
AACAGTGATG GTGAAATTGC TAGTGAATGT GAATGCGATA GTGTCTTTAA CCATTTAGAG 
GAACTGAGAC TTCATCTGGA GCAGGAAATG GGCTTTGAAA AATTCTTTGA GGTTTATGAG 
AAAATAAAGG CTATTCATGA AGATGAAGAT GAAAATATTG AAATTTGTTC AAAAATAGTT 
CAAAATATTT TGGGAAATGA ACATCAGCAT CTTTATGCCA AGATTCTTCA TTTAGTCATG 
GCAGATGGAG CCTACCAAGA AGATAATGAT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_017008252.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863741.1 Homo sapiens NIMA related kinase 1 (NEK1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu95206
Clone ID Related Accession (Same CDS sequence)	XM_017008252.2 , XM_017008251.1 , XM_017008252.1
Accession Version	XM_017008252.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3156bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAACC TGGTACTGAA GATAATATCT GGATCTTTTC CACCTGTGTC TTTGCATTAT 
TCCTATGATC TCCGCAGTTT GGTGTCTCAG TTATTTAAAA GAAATCCTAG GGATAGACCA 
TCAGTCAACT CCATATTGGA GAAAGGTTTT ATAGCCAAAC GCATTGAAAA GTTTCTCTCT 
CCTCAGCTTA TTGCAGAAGA ATTTTGTCTA AAAACATTTT CGAAGTTTGG ATCACAGCCT 
ATACCAGCTA AAAGACCAGC TTCAGGACAA AACTCGATTT CTGTTATGCC TGCTCAGAAA 
ATTACAAAGC CTGCCGCTAA ATATGGAATA CCTTTAGCAT ATAAGAAATA TGGAGATAAA 
AAATTACACG AAAAGAAACC ACTGCAAAAA CATAAACAGG CCCATCAAAC TCCAGAGAAG 
AGAGTGAATA CTGGAGAAGA AAGGAGGAAA ATATCTGAGG AAGCAGCAAG AAAGAGAAGG 
CTGGAATTTA TTGAAAAAGA AAAGAAACAA AAGGATCAGA TTATTAGTTT AATGAAGGCT 
GAACAAATGA AAAGGCAAGA AAAGGAAAGG TTGGAAAGAA TAAATAGGGC CAGGGAACAA 
GGATGGAGAA ATGTGCTAAG TGCTGGTGGA AGTGGTGAAG TAAAGGCTCC TTTTCTGGGC 
AGTGGAGGGA CTATAGCTCC ATCATCTTTT TCTTCTCGAG GACAGTATGA ACATTACCAT 
GCCATTTTTG ACCAAATGCA GCAACAAAGA GCAGAAGATA ATGAAGCTAA ATGGAAAAGA 
GAAATATATG GTCGAGGTCT TCCAGAAAGA GGAATTCTGC CTGGAGTTCG TCCAGGATTT 
CCTTATGGGG CTGCAGGTCA TCACCATTTT CCTGATGCTG ATGATATTAG AAAAACTTTG 
AAAAGATTGA AGGCGGTGTC TAAACAAGCC AATGCAAACA GGCAAAAAGG GCAGCTAGCT 
GTAGAAAGAG CTAAACAAGT AGAAGAGTTC CTGCAGCGAA AACGGGAAGC TATGCAGAAT 
AAAGCTCGAG CCGAAGGACA TATGGTTTAT CTGGCAAGAC TGAGGCAAAT AAGACTACAG 
AATTTCAATG AGCGCCAACA GATTAAAGCC AAACTTCGTG GTGAAAAGAA AGAAGCTAAT 
CATTCTGAAG GACAAGAAGG AAGTGAAGAG GCTGACATGA GGCGCAAAAA AATCGAATCA 
CTGAAGGCCC ATGCAAATGC ACGTGCTGCT GTACTAAAAG AACAACTAGA ACGAAAGAGA 
AAGGAGGCTT ATGAGAGAGA AAAAAAAGTG TGGGAAGAGC ATTTGGTGGC TAAAGGAGTT 
AAGAGTTCTG ATGTTTCTCC ACCTTTGGGA CAGCATGAAA CAGGTGGCTC TCCATCAAAG 
CAACAGATGA GATCTGTTAT TTCTGTAACT TCAGCTTTGA AAGAAGTTGG CGTGGACAGT 
AGTTTAACTG ATACCCGGGA AACTTCAGAA GAGATGCAAA AGACCAACAA TGCTATTTCA 
AGTAAGCGAG AAATACTTCG TAGATTAAAT GAAAATCTTA AAGCTCAAGA AGATGAAAAA 
GGAAAGCAGA ATCTCTCTGA TACTTTTGAG ATAAATGTTC ATGAAGATGC CAAAGAGCAT 
GAAAAAGAAA AATCAGTTTC ATCTGATCGC AAGAAGTGGG AGGCAGGAGG TCAACTTGTG 
ATTCCTCTGG ATGAGTTAAC ACTAGATACA TCCTTCTCTA CAACTGAAAG ACATACAGTG 
GGAGAAGTTA TTAAATTAGG TCCTAATGGA TCTCCAAGAA GAGCCTGGGG GAAAAGTCCG 
ACAGATTCTG TTCTAAAGAT ACTTGGAGAA GCTGAACTAC AACTTCAGAC AGAACTATTA 
GAAAATACAA CTATTAGAAG TGAGATTTCT CCCGAAGGGG AAAAGTACAA ACCCTTAATT 
ACTGGAGAAA AAAAAGTACA ATGTATTTCA CATGAAATAA ACCCATCAGC TATTGTTGAT 
TCTCCTGTTG AGACAAAAAG TCCCGAGTTC AGTGAGGCAT CTCCACAGAT GTCATTGAAA 
CTGGAAGGAA ATTTAGAAGA ACCTGATGAT TTGGAAACAG AAATTCTACA AGAGCCAAGT 
GGAACAAACA AAGATGAGAG CTTGCCATGC ACTATTACTG ATGTGTGGAT TAGTGAGGAA 
AAAGAAACAA AGGAAACTCA GTCGGCAGAT AGGATCACCA TTCAGGAAAA TGAAGTTTCT 
GAAGATGGAG TCTCGAGTAC TGTGGACCAA CTTAGTGACA TTCATATAGA GCCTGGAACC 
AATGATTCTC AGCACTCTAA ATGTGATGTA GATAAGTCTG TGCAACCGGA ACCATTTTTC 
CATAAGGTGG TTCATTCTGA ACACTTGAAC TTAGTCCCTC AAGTTCAATC AGTTCAGTGT 
TCACCAGAAG AATCCTTTGC ATTTCGATCT CACTCGCATT TACCACCAAA AAATAAAAAC 
AAGAATTCCT TGCTGATTGG ACTTTCAACT GGTCTGTTTG ATGCAAACAA CCCAAAGATG 
TTAAGGACAT GTTCACTTCC AGATCTCTCA AAGCTGTTCA GAACCCTTAT GGATGTTCCC 
ACCGTAGGAG ATGTTCGTCA AGACAATCTT GAAATAGATG AAATTGAAGA TGAAAACATT 
AAAGAAGGAC CTTCTGATTC TGAAGACATT GTGTTTGAAG AAACTGACAC AGATTTACAA 
GAGCTGCAGG CCTCGATGGA ACAGTTACTT AGGGAACAAC CTGGTGAAGA ATACAGTGAA 
GAAGAAGAGT CAGTCTTGAA GAACAGTGAT GTGGAGCCAA CTGCAAATGG GACAGATGTG 
GCAGATGAAG ATGACAATCC CAGCAGTGAA AGTGCCCTGA ACGAAGAATG GCACTCAGAT 
AACAGTGATG GTGAAATTGC TAGTGAATGT GAATGCGATA GTGTCTTTAA CCATTTAGAG 
GAACTGAGAC TTCATCTGGA GCAGGAAATG GGCTTTGAAA AATTCTTTGA GGTTTATGAG 
AAAATAAAGG CTATTCATGA AGATGAAGAT GAAAATATTG AAATTTGTTC AAAAATAGTT 
CAAAATATTT TGGGAAATGA ACATCAGCAT CTTTATGCCA AGATTCTTCA TTTAGTCATG 
GCAGATGGAG CCTACCAAGA AGATAATGAT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863741.1
CDS	1124..4279
Translation	MKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKPLQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERGILPGVRPGFPYGAAGHHHFPDADDIRKTLKRLKAVSKQANANRQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMVYLARLRQIRLQNFNERQQIKAKLRGEKKEANHSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQNLSDTFEINVHEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLGPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSEISPEGEKYKPLITGEKKVQCISHEINPSAIVDSPVETKSPEFSEASPQMSLKLEGNLEEPDDLETEILQEPSGTNKDESLPCTITDVWISEEKETKETQSADRITIQENEVSEDGVSSTVDQLSDIHIEPGTNDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGEIASECECDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_017008252.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008252.1

ORF Insert Sequence:

ATGAAAAACC TGGTACTGAA GATAATATCT GGATCTTTTC CACCTGTGTC TTTGCATTAT 
TCCTATGATC TCCGCAGTTT GGTGTCTCAG TTATTTAAAA GAAATCCTAG GGATAGACCA 
TCAGTCAACT CCATATTGGA GAAAGGTTTT ATAGCCAAAC GCATTGAAAA GTTTCTCTCT 
CCTCAGCTTA TTGCAGAAGA ATTTTGTCTA AAAACATTTT CGAAGTTTGG ATCACAGCCT 
ATACCAGCTA AAAGACCAGC TTCAGGACAA AACTCGATTT CTGTTATGCC TGCTCAGAAA 
ATTACAAAGC CTGCCGCTAA ATATGGAATA CCTTTAGCAT ATAAGAAATA TGGAGATAAA 
AAATTACACG AAAAGAAACC ACTGCAAAAA CATAAACAGG CCCATCAAAC TCCAGAGAAG 
AGAGTGAATA CTGGAGAAGA AAGGAGGAAA ATATCTGAGG AAGCAGCAAG AAAGAGAAGG 
CTGGAATTTA TTGAAAAAGA AAAGAAACAA AAGGATCAGA TTATTAGTTT AATGAAGGCT 
GAACAAATGA AAAGGCAAGA AAAGGAAAGG TTGGAAAGAA TAAATAGGGC CAGGGAACAA 
GGATGGAGAA ATGTGCTAAG TGCTGGTGGA AGTGGTGAAG TAAAGGCTCC TTTTCTGGGC 
AGTGGAGGGA CTATAGCTCC ATCATCTTTT TCTTCTCGAG GACAGTATGA ACATTACCAT 
GCCATTTTTG ACCAAATGCA GCAACAAAGA GCAGAAGATA ATGAAGCTAA ATGGAAAAGA 
GAAATATATG GTCGAGGTCT TCCAGAAAGA GGAATTCTGC CTGGAGTTCG TCCAGGATTT 
CCTTATGGGG CTGCAGGTCA TCACCATTTT CCTGATGCTG ATGATATTAG AAAAACTTTG 
AAAAGATTGA AGGCGGTGTC TAAACAAGCC AATGCAAACA GGCAAAAAGG GCAGCTAGCT 
GTAGAAAGAG CTAAACAAGT AGAAGAGTTC CTGCAGCGAA AACGGGAAGC TATGCAGAAT 
AAAGCTCGAG CCGAAGGACA TATGGTTTAT CTGGCAAGAC TGAGGCAAAT AAGACTACAG 
AATTTCAATG AGCGCCAACA GATTAAAGCC AAACTTCGTG GTGAAAAGAA AGAAGCTAAT 
CATTCTGAAG GACAAGAAGG AAGTGAAGAG GCTGACATGA GGCGCAAAAA AATCGAATCA 
CTGAAGGCCC ATGCAAATGC ACGTGCTGCT GTACTAAAAG AACAACTAGA ACGAAAGAGA 
AAGGAGGCTT ATGAGAGAGA AAAAAAAGTG TGGGAAGAGC ATTTGGTGGC TAAAGGAGTT 
AAGAGTTCTG ATGTTTCTCC ACCTTTGGGA CAGCATGAAA CAGGTGGCTC TCCATCAAAG 
CAACAGATGA GATCTGTTAT TTCTGTAACT TCAGCTTTGA AAGAAGTTGG CGTGGACAGT 
AGTTTAACTG ATACCCGGGA AACTTCAGAA GAGATGCAAA AGACCAACAA TGCTATTTCA 
AGTAAGCGAG AAATACTTCG TAGATTAAAT GAAAATCTTA AAGCTCAAGA AGATGAAAAA 
GGAAAGCAGA ATCTCTCTGA TACTTTTGAG ATAAATGTTC ATGAAGATGC CAAAGAGCAT 
GAAAAAGAAA AATCAGTTTC ATCTGATCGC AAGAAGTGGG AGGCAGGAGG TCAACTTGTG 
ATTCCTCTGG ATGAGTTAAC ACTAGATACA TCCTTCTCTA CAACTGAAAG ACATACAGTG 
GGAGAAGTTA TTAAATTAGG TCCTAATGGA TCTCCAAGAA GAGCCTGGGG GAAAAGTCCG 
ACAGATTCTG TTCTAAAGAT ACTTGGAGAA GCTGAACTAC AACTTCAGAC AGAACTATTA 
GAAAATACAA CTATTAGAAG TGAGATTTCT CCCGAAGGGG AAAAGTACAA ACCCTTAATT 
ACTGGAGAAA AAAAAGTACA ATGTATTTCA CATGAAATAA ACCCATCAGC TATTGTTGAT 
TCTCCTGTTG AGACAAAAAG TCCCGAGTTC AGTGAGGCAT CTCCACAGAT GTCATTGAAA 
CTGGAAGGAA ATTTAGAAGA ACCTGATGAT TTGGAAACAG AAATTCTACA AGAGCCAAGT 
GGAACAAACA AAGATGAGAG CTTGCCATGC ACTATTACTG ATGTGTGGAT TAGTGAGGAA 
AAAGAAACAA AGGAAACTCA GTCGGCAGAT AGGATCACCA TTCAGGAAAA TGAAGTTTCT 
GAAGATGGAG TCTCGAGTAC TGTGGACCAA CTTAGTGACA TTCATATAGA GCCTGGAACC 
AATGATTCTC AGCACTCTAA ATGTGATGTA GATAAGTCTG TGCAACCGGA ACCATTTTTC 
CATAAGGTGG TTCATTCTGA ACACTTGAAC TTAGTCCCTC AAGTTCAATC AGTTCAGTGT 
TCACCAGAAG AATCCTTTGC ATTTCGATCT CACTCGCATT TACCACCAAA AAATAAAAAC 
AAGAATTCCT TGCTGATTGG ACTTTCAACT GGTCTGTTTG ATGCAAACAA CCCAAAGATG 
TTAAGGACAT GTTCACTTCC AGATCTCTCA AAGCTGTTCA GAACCCTTAT GGATGTTCCC 
ACCGTAGGAG ATGTTCGTCA AGACAATCTT GAAATAGATG AAATTGAAGA TGAAAACATT 
AAAGAAGGAC CTTCTGATTC TGAAGACATT GTGTTTGAAG AAACTGACAC AGATTTACAA 
GAGCTGCAGG CCTCGATGGA ACAGTTACTT AGGGAACAAC CTGGTGAAGA ATACAGTGAA 
GAAGAAGAGT CAGTCTTGAA GAACAGTGAT GTGGAGCCAA CTGCAAATGG GACAGATGTG 
GCAGATGAAG ATGACAATCC CAGCAGTGAA AGTGCCCTGA ACGAAGAATG GCACTCAGAT 
AACAGTGATG GTGAAATTGC TAGTGAATGT GAATGCGATA GTGTCTTTAA CCATTTAGAG 
GAACTGAGAC TTCATCTGGA GCAGGAAATG GGCTTTGAAA AATTCTTTGA GGTTTATGAG 
AAAATAAAGG CTATTCATGA AGATGAAGAT GAAAATATTG AAATTTGTTC AAAAATAGTT 
CAAAATATTT TGGGAAATGA ACATCAGCAT CTTTATGCCA AGATTCTTCA TTTAGTCATG 
GCAGATGGAG CCTACCAAGA AGATAATGAT GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( NM_001374421.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001361350.1 Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 9, mRNA.

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CloneID	OHu109305
Clone ID Related Accession (Same CDS sequence)	NM_001374421.1
Accession Version	NM_001374421.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek1 isoform 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC084724.6, AC116621.8 and AC116615.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.229564.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
GCTCCTTTTC TGGGCAGTGG AGGGACTATA GCTCCATCAT CTTTTTCTTC TCGAGGACAG 
TATGAACATT ACCATGCCAT TTTTGACCAA ATGCAGCAAC AAAGAGCAGA AGATAATGAA 
GCTAAATGGA AAAGAGAAAT ATATGGTCGA GGTCTTCCAG AAAGAGGAAT TCTGCCTGGA 
GTTCGTCCAG GATTTCCTTA TGGGGCTGCA GGTCATCACC ATTTTCCTGA TGCTGATGAT 
ATTAGAAAAA CTTTGAAAAG ATTGAAGGCG GTGTCTAAAC AAGCCAATGC AAACAGGCAA 
AAAGGGCAGC TAGCTGTAGA AAGAGCTAAA CAAGTAGAAG AGTTCCTGCA GCGAAAACGG 
GAAGCTATGC AGAATAAAGC TCGAGCCGAA GGACATATGG TTTATCTGGC AAGACTGAGG 
CAAATAAGAC TACAGAATTT CAATGAGCGC CAACAGATTA AAGCCAAACT TCGTGGTGAA 
AAGAAAGAAG CTAATCATTC TGAAGGACAA GAAGGAAGTG AAGAGGCTGA CATGAGGCGC 
AAAAAAATCG AATCACTGAA GTTGGTGGCT AAAGGAGTTA AGAGTTCTGA TGTTTCTCCA 
CCTTTGGGAC AGCATGAAAC AGGTGGCTCT CCATCAAAGC AACAGATGAG ATCTGTTATT 
TCTGTAACTT CAGCTTTGAA AGAAGTTGGC GTGGACAGTA GTTTAACTGA TACCCGGGAA 
ACTTCAGAAG AGATGCAAAA GACCAACAAT GCTATTTCAA GTAAGCGAGA AATACTTCGT 
AGATTAAATG AAAATCTTAA AGCTCAAGAA GATGAAAAAG GAAAGCAGAA TCTCTCTGAT 
ACTTTTGAGA TAAATGTTCA TGAAGATGCC AAAGAGCATG AAAAAGAAAA ATCAGTTTCA 
TCTGATCGCA AGAAGTGGGA GGCAGGAGGT CAACTTGTGA TTCCTCTGGA TGAGTTAACA 
CTAGATACAT CCTTCTCTAC AACTGAAAGA CATACAGTGG GAGAAGTTAT TAAATTAGGT 
CCTAATGGAT CTCCAAGAAG AGCCTGGGGG AAAAGTCCGA CAGATTCTGT TCTAAAGATA 
CTTGGAGAAG CTGAACTACA ACTTCAGACA GAACTATTAG AAAATACAAC TATTAGAAGT 
GAACCTGATG ATTTGGAAAC AGAAATTCTA CAAGAGCCAA GTGGAACAAA CAAAGATGAG 
AGCTTGCCAT GCACTATTAC TGATGTGTGG ATTAGTGAGG AAAAAGAAAC AAAGGAAACT 
CAGTCGGCAG ATAGGATCAC CATTCAGGAA AATGAAGTTT CTGAAGATGG AGTCTCGAGT 
ACTGTGGACC AACTTAGTGA CATTCATATA GAGCCTGGAA CCAATGATTC TCAGCACTCT 
AAATGTGATG TAGATAAGTC TGTGCAACCG GAACCATTTT TCCATAAGGT GGTTCATTCT 
GAACACTTGA ACTTAGTCCC TCAAGTTCAA TCAGTTCAGT GTTCACCAGA AGAATCCTTT 
GCATTTCGAT CTCACTCGCA TTTACCACCA AAAAATAAAA ACAAGAATTC CTTGCTGATT 
GGACTTTCAA CTGGTCTGTT TGATGCAAAC AACCCAAAGA TGTTAAGGAC ATGTTCACTT 
CCAGATCTCT CAAAGCTGTT CAGAACCCTT ATGGATGTTC CCACCGTAGG AGATGTTCGT 
CAAGACAATC TTGAAATAGA TGAAATTGAA GATGAAAACA TTAAAGAAGG ACCTTCTGAT 
TCTGAAGACA TTGTGTTTGA AGAAACTGAC ACAGATTTAC AAGAGCTGCA GGCCTCGATG 
GAACAGTTAC TTAGGGAACA ACCTGGTGAA GAATACAGTG AAGAAGAAGA GTCAGTCTTG 
AAGAACAGTG ATGTGGAGCC AACTGCAAAT GGGACAGATG TGGCAGATGA AGATGACAAT 
CCCAGCAGTG AAAGTGCCCT GAACGAAGAA TGGCACTCAG ATAACAGTGA TGGTGAAATT 
GCTAGTGAAT GTGAATGCGA TAGTGTCTTT AACCATTTAG AGGAACTGAG ACTTCATCTG 
GAGCAGGAAA TGGGCTTTGA AAAATTCTTT GAGGTTTATG AGAAAATAAA GGCTATTCAT 
GAAGATGAAG ATGAAAATAT TGAAATTTGT TCAAAAATAG TTCAAAATAT TTTGGGAAAT 
GAACATCAGC ATCTTTATGC CAAGATTCTT CATTTAGTCA TGGCAGATGG AGCCTACCAA 
GAAGATAATG ATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001361350.1
CDS	535..3912
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKPLQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERGILPGVRPGFPYGAAGHHHFPDADDIRKTLKRLKAVSKQANANRQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMVYLARLRQIRLQNFNERQQIKAKLRGEKKEANHSEGQEGSEEADMRRKKIESLKLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQNLSDTFEINVHEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLGPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSEPDDLETEILQEPSGTNKDESLPCTITDVWISEEKETKETQSADRITIQENEVSEDGVSSTVDQLSDIHIEPGTNDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGEIASECECDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	NM_001374421.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001374421.1

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
GCTCCTTTTC TGGGCAGTGG AGGGACTATA GCTCCATCAT CTTTTTCTTC TCGAGGACAG 
TATGAACATT ACCATGCCAT TTTTGACCAA ATGCAGCAAC AAAGAGCAGA AGATAATGAA 
GCTAAATGGA AAAGAGAAAT ATATGGTCGA GGTCTTCCAG AAAGAGGAAT TCTGCCTGGA 
GTTCGTCCAG GATTTCCTTA TGGGGCTGCA GGTCATCACC ATTTTCCTGA TGCTGATGAT 
ATTAGAAAAA CTTTGAAAAG ATTGAAGGCG GTGTCTAAAC AAGCCAATGC AAACAGGCAA 
AAAGGGCAGC TAGCTGTAGA AAGAGCTAAA CAAGTAGAAG AGTTCCTGCA GCGAAAACGG 
GAAGCTATGC AGAATAAAGC TCGAGCCGAA GGACATATGG TTTATCTGGC AAGACTGAGG 
CAAATAAGAC TACAGAATTT CAATGAGCGC CAACAGATTA AAGCCAAACT TCGTGGTGAA 
AAGAAAGAAG CTAATCATTC TGAAGGACAA GAAGGAAGTG AAGAGGCTGA CATGAGGCGC 
AAAAAAATCG AATCACTGAA GTTGGTGGCT AAAGGAGTTA AGAGTTCTGA TGTTTCTCCA 
CCTTTGGGAC AGCATGAAAC AGGTGGCTCT CCATCAAAGC AACAGATGAG ATCTGTTATT 
TCTGTAACTT CAGCTTTGAA AGAAGTTGGC GTGGACAGTA GTTTAACTGA TACCCGGGAA 
ACTTCAGAAG AGATGCAAAA GACCAACAAT GCTATTTCAA GTAAGCGAGA AATACTTCGT 
AGATTAAATG AAAATCTTAA AGCTCAAGAA GATGAAAAAG GAAAGCAGAA TCTCTCTGAT 
ACTTTTGAGA TAAATGTTCA TGAAGATGCC AAAGAGCATG AAAAAGAAAA ATCAGTTTCA 
TCTGATCGCA AGAAGTGGGA GGCAGGAGGT CAACTTGTGA TTCCTCTGGA TGAGTTAACA 
CTAGATACAT CCTTCTCTAC AACTGAAAGA CATACAGTGG GAGAAGTTAT TAAATTAGGT 
CCTAATGGAT CTCCAAGAAG AGCCTGGGGG AAAAGTCCGA CAGATTCTGT TCTAAAGATA 
CTTGGAGAAG CTGAACTACA ACTTCAGACA GAACTATTAG AAAATACAAC TATTAGAAGT 
GAACCTGATG ATTTGGAAAC AGAAATTCTA CAAGAGCCAA GTGGAACAAA CAAAGATGAG 
AGCTTGCCAT GCACTATTAC TGATGTGTGG ATTAGTGAGG AAAAAGAAAC AAAGGAAACT 
CAGTCGGCAG ATAGGATCAC CATTCAGGAA AATGAAGTTT CTGAAGATGG AGTCTCGAGT 
ACTGTGGACC AACTTAGTGA CATTCATATA GAGCCTGGAA CCAATGATTC TCAGCACTCT 
AAATGTGATG TAGATAAGTC TGTGCAACCG GAACCATTTT TCCATAAGGT GGTTCATTCT 
GAACACTTGA ACTTAGTCCC TCAAGTTCAA TCAGTTCAGT GTTCACCAGA AGAATCCTTT 
GCATTTCGAT CTCACTCGCA TTTACCACCA AAAAATAAAA ACAAGAATTC CTTGCTGATT 
GGACTTTCAA CTGGTCTGTT TGATGCAAAC AACCCAAAGA TGTTAAGGAC ATGTTCACTT 
CCAGATCTCT CAAAGCTGTT CAGAACCCTT ATGGATGTTC CCACCGTAGG AGATGTTCGT 
CAAGACAATC TTGAAATAGA TGAAATTGAA GATGAAAACA TTAAAGAAGG ACCTTCTGAT 
TCTGAAGACA TTGTGTTTGA AGAAACTGAC ACAGATTTAC AAGAGCTGCA GGCCTCGATG 
GAACAGTTAC TTAGGGAACA ACCTGGTGAA GAATACAGTG AAGAAGAAGA GTCAGTCTTG 
AAGAACAGTG ATGTGGAGCC AACTGCAAAT GGGACAGATG TGGCAGATGA AGATGACAAT 
CCCAGCAGTG AAAGTGCCCT GAACGAAGAA TGGCACTCAG ATAACAGTGA TGGTGAAATT 
GCTAGTGAAT GTGAATGCGA TAGTGTCTTT AACCATTTAG AGGAACTGAG ACTTCATCTG 
GAGCAGGAAA TGGGCTTTGA AAAATTCTTT GAGGTTTATG AGAAAATAAA GGCTATTCAT 
GAAGATGAAG ATGAAAATAT TGAAATTTGT TCAAAAATAG TTCAAAATAT TTTGGGAAAT 
GAACATCAGC ATCTTTATGC CAAGATTCTT CATTTAGTCA TGGCAGATGG AGCCTACCAA 
GAAGATAATG ATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( NM_001374420.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001361349.1 Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OHu109306
Clone ID Related Accession (Same CDS sequence)	NM_001374420.1
Accession Version	NM_001374420.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek1 isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC084724.6, AC116621.8 and AC116615.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.61794.1, SRR1660805.147357.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
TTGGAAAGAA TAAATAGGGC CAGGGAACAA GGATGGAGAA ATGTGCTAAG TGCTGGTGGA 
AGTGGTGAAG TAAAGGCTCC TTTTCTGGGC AGTGGAGGGA CTATAGCTCC ATCATCTTTT 
TCTTCTCGAG GACAGTATGA ACATTACCAT GCCATTTTTG ACCAAATGCA GCAACAAAGA 
GCAGAAGATA ATGAAGCTAA ATGGAAAAGA GAAATATATG GTCGAGGTCT TCCAGAAAGA 
GGAATTCTGC CTGGAGTTCG TCCAGGATTT CCTTATGGGG CTGCAGGTCA TCACCATTTT 
CCTGATGCTG ATGATATTAG AAAAACTTTG AAAAGATTGA AGGCGGTGTC TAAACAAGCC 
AATGCAAACA GGCAAAAAGG GCAGCTAGCT GTAGAAAGAG CTAAACAAGT AGAAGAGTTC 
CTGCAGCGAA AACGGGAAGC TATGCAGAAT AAAGCTCGAG CCGAAGGACA TATGGTTTAT 
CTGGCAAGAC TGAGGCAAAT AAGACTACAG AATTTCAATG AGCGCCAACA GATTAAAGCC 
AAACTTCGTG GTGAAAAGAA AGAAGCTAAT CATTCTGAAG GACAAGAAGG AAGTGAAGAG 
GCTGACATGA GGCGCAAAAA AATCGAATCA CTGAAGGCCC ATGCAAATGC ACGTGCTGCT 
GTACTAAAAG AACAACTAGA ACGAAAGAGA AAGGAGGCTT ATGAGAGAGA AAAAAAAGTG 
TGGGAAGAGC ATTTGGTGGC TAAAGGAGTT AAGAGTTCTG ATGTTTCTCC ACCTTTGGGA 
CAGCATGAAA CAGGTGGCTC TCCATCAAAG CAACAGATGA GATCTGTTAT TTCTGTAACT 
TCAGCTTTGA AAGAAGTTGG CGTGGACAGT AGTTTAACTG ATACCCGGGA AACTTCAGAA 
GAGATGCAAA AGACCAACAA TGCTATTTCA AGTAAGCGAG AAATACTTCG TAGATTAAAT 
GAAAATCTTA AAGCTCAAGA AGATGAAAAA GGAAAGCAGA ATCTCTCTGA TACTTTTGAG 
ATAAATGTTC ATGAAGATGC CAAAGAGCAT GAAAAAGAAA AATCAGTTTC ATCTGATCGC 
AAGAAGTGGG AGGCAGGAGG TCAACTTGTG ATTCCTCTGG ATGAGTTAAC ACTAGATACA 
TCCTTCTCTA CAACTGAAAG ACATACAGTG GGAGAAGTTA TTAAATTAGG TCCTAATGGA 
TCTCCAAGAA GAGCCTGGGG GAAAAGTCCG ACAGATTCTG TTCTAAAGAT ACTTGGAGAA 
GCTGAACTAC AACTTCAGAC AGAACTATTA GAAAATACAA CTATTAGAAG TGAGATTTCT 
CCCGAAGGGG AAAAGTACAA ACCCTTAATT ACTGGAGAAA AAAAAGTACA ATGTATTTCA 
CATGAAATAA ACCCATCAGC TATTGTTGAT TCTCCTGTTG AGACAAAAAG TCCCGAGTTC 
AGTGAGGCAT CTCCACAGAT GTCATTGAAA CTGGAAGGAA ATTTAGAAGA ACCTGATGAT 
TTGGAAACAG AAATTCTACA AGAGCCAAGT GGAACAAACA AAGATGAGAG CTTGCCATGC 
ACTATTACTG ATGTGTGGAT TAGTGAGGAA AAAGAAACAA AGGAAACTCA GTCGGCAGAT 
AGGATCACCA TTCAGGAAAA TGAAGTTTCT GAAGATGGAG TCTCGAGTAC TGTGGACCAA 
CTTAGTGACA TTCATATAGA GCCTGGAACC AATGATTCTC AGCACTCTAA ATGTGATGTA 
GATAAGTCTG TGCAACCGGA ACCATTTTTC CATAAGGTGG TTCATTCTGA ACACTTGAAC 
TTAGTCCCTC AAGTTCAATC AGTTCAGTGT TCACCAGAAG AATCCTTTGC ATTTCGATCT 
CACTCGCATT TACCACCAAA AAATAAAAAC AAGAATTCCT TGCTGATTGG ACTTTCAACT 
GGTCTGTTTG ATGCAAACAA CCCAAAGATG TTAAGGACAT GTTCACTTCC AGATCTCTCA 
AAGCTGTTCA GAACCCTTAT GGATGTTCCC ACCGTAGGAG ATGTTCGTCA AGACAATCTT 
GAAATAGATG AAATTGAAGA TGAAAACATT AAAGAAGGAC CTTCTGATTC TGAAGACATT 
GTGTTTGAAG AAACTGACAC AGATTTACAA GAGCTGCAGG CCTCGATGGA ACAGTTACTT 
AGGGAACAAC CTGGTGAAGA ATACAGTGAA GAAGAAGAGT CAGTCTTGAA GAACAGTGAT 
GTGGAGCCAA CTGCAAATGG GACAGATGTG GCAGATGAAG ATGACAATCC CAGCAGTGAA 
AGTGCCCTGA ACGAAGAATG GCACTCAGAT AACAGTGATG GTGAAATTGC TAGTGAATGT 
GAATGCGATA GTGTCTTTAA CCATTTAGAG GAACTGAGAC TTCATCTGGA GCAGGAAATG 
GGCTTTGAAA AATTCTTTGA GGTTTATGAG AAAATAAAGG CTATTCATGA AGATGAAGAT 
GAAAATATTG AAATTTGTTC AAAAATAGTT CAAAATATTT TGGGAAATGA ACATCAGCAT 
CTTTATGCCA AGATTCTTCA TTTAGTCATG GCAGATGGAG CCTACCAAGA AGATAATGAT 
GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001361349.1
CDS	535..4260
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKPLQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQLERINRAREQGWRNVLSAGGSGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERGILPGVRPGFPYGAAGHHHFPDADDIRKTLKRLKAVSKQANANRQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMVYLARLRQIRLQNFNERQQIKAKLRGEKKEANHSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQNLSDTFEINVHEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLGPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSEISPEGEKYKPLITGEKKVQCISHEINPSAIVDSPVETKSPEFSEASPQMSLKLEGNLEEPDDLETEILQEPSGTNKDESLPCTITDVWISEEKETKETQSADRITIQENEVSEDGVSSTVDQLSDIHIEPGTNDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGEIASECECDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	NM_001374420.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001374420.1

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
TTGGAAAGAA TAAATAGGGC CAGGGAACAA GGATGGAGAA ATGTGCTAAG TGCTGGTGGA 
AGTGGTGAAG TAAAGGCTCC TTTTCTGGGC AGTGGAGGGA CTATAGCTCC ATCATCTTTT 
TCTTCTCGAG GACAGTATGA ACATTACCAT GCCATTTTTG ACCAAATGCA GCAACAAAGA 
GCAGAAGATA ATGAAGCTAA ATGGAAAAGA GAAATATATG GTCGAGGTCT TCCAGAAAGA 
GGAATTCTGC CTGGAGTTCG TCCAGGATTT CCTTATGGGG CTGCAGGTCA TCACCATTTT 
CCTGATGCTG ATGATATTAG AAAAACTTTG AAAAGATTGA AGGCGGTGTC TAAACAAGCC 
AATGCAAACA GGCAAAAAGG GCAGCTAGCT GTAGAAAGAG CTAAACAAGT AGAAGAGTTC 
CTGCAGCGAA AACGGGAAGC TATGCAGAAT AAAGCTCGAG CCGAAGGACA TATGGTTTAT 
CTGGCAAGAC TGAGGCAAAT AAGACTACAG AATTTCAATG AGCGCCAACA GATTAAAGCC 
AAACTTCGTG GTGAAAAGAA AGAAGCTAAT CATTCTGAAG GACAAGAAGG AAGTGAAGAG 
GCTGACATGA GGCGCAAAAA AATCGAATCA CTGAAGGCCC ATGCAAATGC ACGTGCTGCT 
GTACTAAAAG AACAACTAGA ACGAAAGAGA AAGGAGGCTT ATGAGAGAGA AAAAAAAGTG 
TGGGAAGAGC ATTTGGTGGC TAAAGGAGTT AAGAGTTCTG ATGTTTCTCC ACCTTTGGGA 
CAGCATGAAA CAGGTGGCTC TCCATCAAAG CAACAGATGA GATCTGTTAT TTCTGTAACT 
TCAGCTTTGA AAGAAGTTGG CGTGGACAGT AGTTTAACTG ATACCCGGGA AACTTCAGAA 
GAGATGCAAA AGACCAACAA TGCTATTTCA AGTAAGCGAG AAATACTTCG TAGATTAAAT 
GAAAATCTTA AAGCTCAAGA AGATGAAAAA GGAAAGCAGA ATCTCTCTGA TACTTTTGAG 
ATAAATGTTC ATGAAGATGC CAAAGAGCAT GAAAAAGAAA AATCAGTTTC ATCTGATCGC 
AAGAAGTGGG AGGCAGGAGG TCAACTTGTG ATTCCTCTGG ATGAGTTAAC ACTAGATACA 
TCCTTCTCTA CAACTGAAAG ACATACAGTG GGAGAAGTTA TTAAATTAGG TCCTAATGGA 
TCTCCAAGAA GAGCCTGGGG GAAAAGTCCG ACAGATTCTG TTCTAAAGAT ACTTGGAGAA 
GCTGAACTAC AACTTCAGAC AGAACTATTA GAAAATACAA CTATTAGAAG TGAGATTTCT 
CCCGAAGGGG AAAAGTACAA ACCCTTAATT ACTGGAGAAA AAAAAGTACA ATGTATTTCA 
CATGAAATAA ACCCATCAGC TATTGTTGAT TCTCCTGTTG AGACAAAAAG TCCCGAGTTC 
AGTGAGGCAT CTCCACAGAT GTCATTGAAA CTGGAAGGAA ATTTAGAAGA ACCTGATGAT 
TTGGAAACAG AAATTCTACA AGAGCCAAGT GGAACAAACA AAGATGAGAG CTTGCCATGC 
ACTATTACTG ATGTGTGGAT TAGTGAGGAA AAAGAAACAA AGGAAACTCA GTCGGCAGAT 
AGGATCACCA TTCAGGAAAA TGAAGTTTCT GAAGATGGAG TCTCGAGTAC TGTGGACCAA 
CTTAGTGACA TTCATATAGA GCCTGGAACC AATGATTCTC AGCACTCTAA ATGTGATGTA 
GATAAGTCTG TGCAACCGGA ACCATTTTTC CATAAGGTGG TTCATTCTGA ACACTTGAAC 
TTAGTCCCTC AAGTTCAATC AGTTCAGTGT TCACCAGAAG AATCCTTTGC ATTTCGATCT 
CACTCGCATT TACCACCAAA AAATAAAAAC AAGAATTCCT TGCTGATTGG ACTTTCAACT 
GGTCTGTTTG ATGCAAACAA CCCAAAGATG TTAAGGACAT GTTCACTTCC AGATCTCTCA 
AAGCTGTTCA GAACCCTTAT GGATGTTCCC ACCGTAGGAG ATGTTCGTCA AGACAATCTT 
GAAATAGATG AAATTGAAGA TGAAAACATT AAAGAAGGAC CTTCTGATTC TGAAGACATT 
GTGTTTGAAG AAACTGACAC AGATTTACAA GAGCTGCAGG CCTCGATGGA ACAGTTACTT 
AGGGAACAAC CTGGTGAAGA ATACAGTGAA GAAGAAGAGT CAGTCTTGAA GAACAGTGAT 
GTGGAGCCAA CTGCAAATGG GACAGATGTG GCAGATGAAG ATGACAATCC CAGCAGTGAA 
AGTGCCCTGA ACGAAGAATG GCACTCAGAT AACAGTGATG GTGAAATTGC TAGTGAATGT 
GAATGCGATA GTGTCTTTAA CCATTTAGAG GAACTGAGAC TTCATCTGGA GCAGGAAATG 
GGCTTTGAAA AATTCTTTGA GGTTTATGAG AAAATAAAGG CTATTCATGA AGATGAAGAT 
GAAAATATTG AAATTTGTTC AAAAATAGTT CAAAATATTT TGGGAAATGA ACATCAGCAT 
CTTTATGCCA AGATTCTTCA TTTAGTCATG GCAGATGGAG CCTACCAAGA AGATAATGAT 
GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( NM_001199398.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001186327.1 Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu11596
Clone ID Related Accession (Same CDS sequence)	NM_001199398.1 , NM_001199398.2
Accession Version	NM_001199398.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA697159.1, AL050385.1, AC084724.6, AC116621.8, AC116615.5, AK025658.1 and AI984346.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL050385.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTGGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG GCAAAAAGGG 
CAGCTAGCTG TAGAAAGAGC TAAACAAGTA GAAGAGTTCC TGCAGCGAAA ACGGGAAGCT 
ATGCAGAATA AAGCTCGAGC CGAAGGACAT ATGGGAATCC TGCAAAACCT GGCAGCTATG 
TATGGAGGCA GGCCCAGCTC TTCAAGAGGA GGGAAGCCAA GAAACAAAGA GGAAGAGGTT 
TATCTGGCAA GACTGAGGCA AATAAGACTA CAGAATTTCA ATGAGCGCCA ACAGATTAAA 
GCCAAACTTC GTGGTGAAAA GAAAGAAGCT AATCATTCTG AAGGACAAGA AGGAAGTGAA 
GAGGCTGACA TGAGGCGCAA AAAAATCGAA TCACTGAAGG CCCATGCAAA TGCACGTGCT 
GCTGTACTAA AAGAACAACT AGAACGAAAG AGAAAGGAGG CTTATGAGAG AGAAAAAAAA 
GTGTGGGAAG AGCATTTGGT GGCTAAAGGA GTTAAGAGTT CTGATGTTTC TCCACCTTTG 
GGACAGCATG AAACAGGTGG CTCTCCATCA AAGCAACAGA TGAGATCTGT TATTTCTGTA 
ACTTCAGCTT TGAAAGAAGT TGGCGTGGAC AGTAGTTTAA CTGATACCCG GGAAACTTCA 
GAAGAGATGC AAAAGACCAA CAATGCTATT TCAAGTAAGC GAGAAATACT TCGTAGATTA 
AATGAAAATC TTAAAGCTCA AGAAGATGAA AAAGGAAAGC AGAATCTCTC TGATACTTTT 
GAGATAAATG TTCATGAAGA TGCCAAAGAG CATGAAAAAG AAAAATCAGT TTCATCTGAT 
CGCAAGAAGT GGGAGGCAGG AGGTCAACTT GTGATTCCTC TGGATGAGTT AACACTAGAT 
ACATCCTTCT CTACAACTGA AAGACATACA GTGGGAGAAG TTATTAAATT AGGTCCTAAT 
GGATCTCCAA GAAGAGCCTG GGGGAAAAGT CCGACAGATT CTGTTCTAAA GATACTTGGA 
GAAGCTGAAC TACAACTTCA GACAGAACTA TTAGAAAATA CAACTATTAG AAGTGAGATT 
TCTCCCGAAG GGGAAAAGTA CAAACCCTTA ATTACTGGAG AAAAAAAAGT ACAATGTATT 
TCACATGAAA TAAACCCATC AGCTATTGTT GATTCTCCTG TTGAGACAAA AAGTCCCGAG 
TTCAGTGAGG CATCTCCACA GATGTCATTG AAACTGGAAG GAAATTTAGA AGAACCTGAT 
GATTTGGAAA CAGAAATTCT ACAAGAGCCA AGTGGAACAA ACAAAGATGA GAGCTTGCCA 
TGCACTATTA CTGATGTGTG GATTAGTGAG GAAAAAGAAA CAAAGGAAAC TCAGTCGGCA 
GATAGGATCA CCATTCAGGA AAATGAAGTT TCTGAAGATG GAGTCTCGAG TACTGTGGAC 
CAACTTAGTG ACATTCATAT AGAGCCTGGA ACCAATGATT CTCAGCACTC TAAATGTGAT 
GTAGATAAGT CTGTGCAACC GGAACCATTT TTCCATAAGG TGGTTCATTC TGAACACTTG 
AACTTAGTCC CTCAAGTTCA ATCAGTTCAG TGTTCACCAG AAGAATCCTT TGCATTTCGA 
TCTCACTCGC ATTTACCACC AAAAAATAAA AACAAGAATT CCTTGCTGAT TGGACTTTCA 
ACTGGTCTGT TTGATGCAAA CAACCCAAAG ATGTTAAGGA CATGTTCACT TCCAGATCTC 
TCAAAGCTGT TCAGAACCCT TATGGATGTT CCCACCGTAG GAGATGTTCG TCAAGACAAT 
CTTGAAATAG ATGAAATTGA AGATGAAAAC ATTAAAGAAG GACCTTCTGA TTCTGAAGAC 
ATTGTGTTTG AAGAAACTGA CACAGATTTA CAAGAGCTGC AGGCCTCGAT GGAACAGTTA 
CTTAGGGAAC AACCTGGTGA AGAATACAGT GAAGAAGAAG AGTCAGTCTT GAAGAACAGT 
GATGTGGAGC CAACTGCAAA TGGGACAGAT GTGGCAGATG AAGATGACAA TCCCAGCAGT 
GAAAGTGCCC TGAACGAAGA ATGGCACTCA GATAACAGTG ATGGTGAAAT TGCTAGTGAA 
TGTGAATGCG ATAGTGTCTT TAACCATTTA GAGGAACTGA GACTTCATCT GGAGCAGGAA 
ATGGGCTTTG AAAAATTCTT TGAGGTTTAT GAGAAAATAA AGGCTATTCA TGAAGATGAA 
GATGAAAATA TTGAAATTTG TTCAAAAATA GTTCAAAATA TTTTGGGAAA TGAACATCAG 
CATCTTTATG CCAAGATTCT TCATTTAGTC ATGGCAGATG GAGCCTACCA AGAAGATAAT 
GATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186327.1
CDS	579..4307
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKPLQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMGILQNLAAMYGGRPSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIKAKLRGEKKEANHSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQNLSDTFEINVHEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLGPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSEISPEGEKYKPLITGEKKVQCISHEINPSAIVDSPVETKSPEFSEASPQMSLKLEGNLEEPDDLETEILQEPSGTNKDESLPCTITDVWISEEKETKETQSADRITIQENEVSEDGVSSTVDQLSDIHIEPGTNDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGEIASECECDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	NM_001199398.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199398.1

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTGGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG GCAAAAAGGG 
CAGCTAGCTG TAGAAAGAGC TAAACAAGTA GAAGAGTTCC TGCAGCGAAA ACGGGAAGCT 
ATGCAGAATA AAGCTCGAGC CGAAGGACAT ATGGGAATCC TGCAAAACCT GGCAGCTATG 
TATGGAGGCA GGCCCAGCTC TTCAAGAGGA GGGAAGCCAA GAAACAAAGA GGAAGAGGTT 
TATCTGGCAA GACTGAGGCA AATAAGACTA CAGAATTTCA ATGAGCGCCA ACAGATTAAA 
GCCAAACTTC GTGGTGAAAA GAAAGAAGCT AATCATTCTG AAGGACAAGA AGGAAGTGAA 
GAGGCTGACA TGAGGCGCAA AAAAATCGAA TCACTGAAGG CCCATGCAAA TGCACGTGCT 
GCTGTACTAA AAGAACAACT AGAACGAAAG AGAAAGGAGG CTTATGAGAG AGAAAAAAAA 
GTGTGGGAAG AGCATTTGGT GGCTAAAGGA GTTAAGAGTT CTGATGTTTC TCCACCTTTG 
GGACAGCATG AAACAGGTGG CTCTCCATCA AAGCAACAGA TGAGATCTGT TATTTCTGTA 
ACTTCAGCTT TGAAAGAAGT TGGCGTGGAC AGTAGTTTAA CTGATACCCG GGAAACTTCA 
GAAGAGATGC AAAAGACCAA CAATGCTATT TCAAGTAAGC GAGAAATACT TCGTAGATTA 
AATGAAAATC TTAAAGCTCA AGAAGATGAA AAAGGAAAGC AGAATCTCTC TGATACTTTT 
GAGATAAATG TTCATGAAGA TGCCAAAGAG CATGAAAAAG AAAAATCAGT TTCATCTGAT 
CGCAAGAAGT GGGAGGCAGG AGGTCAACTT GTGATTCCTC TGGATGAGTT AACACTAGAT 
ACATCCTTCT CTACAACTGA AAGACATACA GTGGGAGAAG TTATTAAATT AGGTCCTAAT 
GGATCTCCAA GAAGAGCCTG GGGGAAAAGT CCGACAGATT CTGTTCTAAA GATACTTGGA 
GAAGCTGAAC TACAACTTCA GACAGAACTA TTAGAAAATA CAACTATTAG AAGTGAGATT 
TCTCCCGAAG GGGAAAAGTA CAAACCCTTA ATTACTGGAG AAAAAAAAGT ACAATGTATT 
TCACATGAAA TAAACCCATC AGCTATTGTT GATTCTCCTG TTGAGACAAA AAGTCCCGAG 
TTCAGTGAGG CATCTCCACA GATGTCATTG AAACTGGAAG GAAATTTAGA AGAACCTGAT 
GATTTGGAAA CAGAAATTCT ACAAGAGCCA AGTGGAACAA ACAAAGATGA GAGCTTGCCA 
TGCACTATTA CTGATGTGTG GATTAGTGAG GAAAAAGAAA CAAAGGAAAC TCAGTCGGCA 
GATAGGATCA CCATTCAGGA AAATGAAGTT TCTGAAGATG GAGTCTCGAG TACTGTGGAC 
CAACTTAGTG ACATTCATAT AGAGCCTGGA ACCAATGATT CTCAGCACTC TAAATGTGAT 
GTAGATAAGT CTGTGCAACC GGAACCATTT TTCCATAAGG TGGTTCATTC TGAACACTTG 
AACTTAGTCC CTCAAGTTCA ATCAGTTCAG TGTTCACCAG AAGAATCCTT TGCATTTCGA 
TCTCACTCGC ATTTACCACC AAAAAATAAA AACAAGAATT CCTTGCTGAT TGGACTTTCA 
ACTGGTCTGT TTGATGCAAA CAACCCAAAG ATGTTAAGGA CATGTTCACT TCCAGATCTC 
TCAAAGCTGT TCAGAACCCT TATGGATGTT CCCACCGTAG GAGATGTTCG TCAAGACAAT 
CTTGAAATAG ATGAAATTGA AGATGAAAAC ATTAAAGAAG GACCTTCTGA TTCTGAAGAC 
ATTGTGTTTG AAGAAACTGA CACAGATTTA CAAGAGCTGC AGGCCTCGAT GGAACAGTTA 
CTTAGGGAAC AACCTGGTGA AGAATACAGT GAAGAAGAAG AGTCAGTCTT GAAGAACAGT 
GATGTGGAGC CAACTGCAAA TGGGACAGAT GTGGCAGATG AAGATGACAA TCCCAGCAGT 
GAAAGTGCCC TGAACGAAGA ATGGCACTCA GATAACAGTG ATGGTGAAAT TGCTAGTGAA 
TGTGAATGCG ATAGTGTCTT TAACCATTTA GAGGAACTGA GACTTCATCT GGAGCAGGAA 
ATGGGCTTTG AAAAATTCTT TGAGGTTTAT GAGAAAATAA AGGCTATTCA TGAAGATGAA 
GATGAAAATA TTGAAATTTG TTCAAAAATA GTTCAAAATA TTTTGGGAAA TGAACATCAG 
CATCTTTATG CCAAGATTCT TCATTTAGTC ATGGCAGATG GAGCCTACCA AGAAGATAAT 
GATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( NM_001199398.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001186327.1 Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 3, mRNA.

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CloneID	OHu11596
Clone ID Related Accession (Same CDS sequence)	NM_001199398.1 , NM_001199398.2
Accession Version	NM_001199398.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA697159.1, AL050385.1, AC084724.6, AC116621.8, AC116615.5, AK025658.1 and AI984346.1. On Jun 2, 2019 this sequence version replaced NM_001199398.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL050385.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTGGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG GCAAAAAGGG 
CAGCTAGCTG TAGAAAGAGC TAAACAAGTA GAAGAGTTCC TGCAGCGAAA ACGGGAAGCT 
ATGCAGAATA AAGCTCGAGC CGAAGGACAT ATGGGAATCC TGCAAAACCT GGCAGCTATG 
TATGGAGGCA GGCCCAGCTC TTCAAGAGGA GGGAAGCCAA GAAACAAAGA GGAAGAGGTT 
TATCTGGCAA GACTGAGGCA AATAAGACTA CAGAATTTCA ATGAGCGCCA ACAGATTAAA 
GCCAAACTTC GTGGTGAAAA GAAAGAAGCT AATCATTCTG AAGGACAAGA AGGAAGTGAA 
GAGGCTGACA TGAGGCGCAA AAAAATCGAA TCACTGAAGG CCCATGCAAA TGCACGTGCT 
GCTGTACTAA AAGAACAACT AGAACGAAAG AGAAAGGAGG CTTATGAGAG AGAAAAAAAA 
GTGTGGGAAG AGCATTTGGT GGCTAAAGGA GTTAAGAGTT CTGATGTTTC TCCACCTTTG 
GGACAGCATG AAACAGGTGG CTCTCCATCA AAGCAACAGA TGAGATCTGT TATTTCTGTA 
ACTTCAGCTT TGAAAGAAGT TGGCGTGGAC AGTAGTTTAA CTGATACCCG GGAAACTTCA 
GAAGAGATGC AAAAGACCAA CAATGCTATT TCAAGTAAGC GAGAAATACT TCGTAGATTA 
AATGAAAATC TTAAAGCTCA AGAAGATGAA AAAGGAAAGC AGAATCTCTC TGATACTTTT 
GAGATAAATG TTCATGAAGA TGCCAAAGAG CATGAAAAAG AAAAATCAGT TTCATCTGAT 
CGCAAGAAGT GGGAGGCAGG AGGTCAACTT GTGATTCCTC TGGATGAGTT AACACTAGAT 
ACATCCTTCT CTACAACTGA AAGACATACA GTGGGAGAAG TTATTAAATT AGGTCCTAAT 
GGATCTCCAA GAAGAGCCTG GGGGAAAAGT CCGACAGATT CTGTTCTAAA GATACTTGGA 
GAAGCTGAAC TACAACTTCA GACAGAACTA TTAGAAAATA CAACTATTAG AAGTGAGATT 
TCTCCCGAAG GGGAAAAGTA CAAACCCTTA ATTACTGGAG AAAAAAAAGT ACAATGTATT 
TCACATGAAA TAAACCCATC AGCTATTGTT GATTCTCCTG TTGAGACAAA AAGTCCCGAG 
TTCAGTGAGG CATCTCCACA GATGTCATTG AAACTGGAAG GAAATTTAGA AGAACCTGAT 
GATTTGGAAA CAGAAATTCT ACAAGAGCCA AGTGGAACAA ACAAAGATGA GAGCTTGCCA 
TGCACTATTA CTGATGTGTG GATTAGTGAG GAAAAAGAAA CAAAGGAAAC TCAGTCGGCA 
GATAGGATCA CCATTCAGGA AAATGAAGTT TCTGAAGATG GAGTCTCGAG TACTGTGGAC 
CAACTTAGTG ACATTCATAT AGAGCCTGGA ACCAATGATT CTCAGCACTC TAAATGTGAT 
GTAGATAAGT CTGTGCAACC GGAACCATTT TTCCATAAGG TGGTTCATTC TGAACACTTG 
AACTTAGTCC CTCAAGTTCA ATCAGTTCAG TGTTCACCAG AAGAATCCTT TGCATTTCGA 
TCTCACTCGC ATTTACCACC AAAAAATAAA AACAAGAATT CCTTGCTGAT TGGACTTTCA 
ACTGGTCTGT TTGATGCAAA CAACCCAAAG ATGTTAAGGA CATGTTCACT TCCAGATCTC 
TCAAAGCTGT TCAGAACCCT TATGGATGTT CCCACCGTAG GAGATGTTCG TCAAGACAAT 
CTTGAAATAG ATGAAATTGA AGATGAAAAC ATTAAAGAAG GACCTTCTGA TTCTGAAGAC 
ATTGTGTTTG AAGAAACTGA CACAGATTTA CAAGAGCTGC AGGCCTCGAT GGAACAGTTA 
CTTAGGGAAC AACCTGGTGA AGAATACAGT GAAGAAGAAG AGTCAGTCTT GAAGAACAGT 
GATGTGGAGC CAACTGCAAA TGGGACAGAT GTGGCAGATG AAGATGACAA TCCCAGCAGT 
GAAAGTGCCC TGAACGAAGA ATGGCACTCA GATAACAGTG ATGGTGAAAT TGCTAGTGAA 
TGTGAATGCG ATAGTGTCTT TAACCATTTA GAGGAACTGA GACTTCATCT GGAGCAGGAA 
ATGGGCTTTG AAAAATTCTT TGAGGTTTAT GAGAAAATAA AGGCTATTCA TGAAGATGAA 
GATGAAAATA TTGAAATTTG TTCAAAAATA GTTCAAAATA TTTTGGGAAA TGAACATCAG 
CATCTTTATG CCAAGATTCT TCATTTAGTC ATGGCAGATG GAGCCTACCA AGAAGATAAT 
GATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186327.1
CDS	535..4263
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKPLQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMGILQNLAAMYGGRPSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIKAKLRGEKKEANHSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQNLSDTFEINVHEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLGPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSEISPEGEKYKPLITGEKKVQCISHEINPSAIVDSPVETKSPEFSEASPQMSLKLEGNLEEPDDLETEILQEPSGTNKDESLPCTITDVWISEEKETKETQSADRITIQENEVSEDGVSSTVDQLSDIHIEPGTNDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGEIASECECDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	NM_001199398.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199398.2

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTGGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG GCAAAAAGGG 
CAGCTAGCTG TAGAAAGAGC TAAACAAGTA GAAGAGTTCC TGCAGCGAAA ACGGGAAGCT 
ATGCAGAATA AAGCTCGAGC CGAAGGACAT ATGGGAATCC TGCAAAACCT GGCAGCTATG 
TATGGAGGCA GGCCCAGCTC TTCAAGAGGA GGGAAGCCAA GAAACAAAGA GGAAGAGGTT 
TATCTGGCAA GACTGAGGCA AATAAGACTA CAGAATTTCA ATGAGCGCCA ACAGATTAAA 
GCCAAACTTC GTGGTGAAAA GAAAGAAGCT AATCATTCTG AAGGACAAGA AGGAAGTGAA 
GAGGCTGACA TGAGGCGCAA AAAAATCGAA TCACTGAAGG CCCATGCAAA TGCACGTGCT 
GCTGTACTAA AAGAACAACT AGAACGAAAG AGAAAGGAGG CTTATGAGAG AGAAAAAAAA 
GTGTGGGAAG AGCATTTGGT GGCTAAAGGA GTTAAGAGTT CTGATGTTTC TCCACCTTTG 
GGACAGCATG AAACAGGTGG CTCTCCATCA AAGCAACAGA TGAGATCTGT TATTTCTGTA 
ACTTCAGCTT TGAAAGAAGT TGGCGTGGAC AGTAGTTTAA CTGATACCCG GGAAACTTCA 
GAAGAGATGC AAAAGACCAA CAATGCTATT TCAAGTAAGC GAGAAATACT TCGTAGATTA 
AATGAAAATC TTAAAGCTCA AGAAGATGAA AAAGGAAAGC AGAATCTCTC TGATACTTTT 
GAGATAAATG TTCATGAAGA TGCCAAAGAG CATGAAAAAG AAAAATCAGT TTCATCTGAT 
CGCAAGAAGT GGGAGGCAGG AGGTCAACTT GTGATTCCTC TGGATGAGTT AACACTAGAT 
ACATCCTTCT CTACAACTGA AAGACATACA GTGGGAGAAG TTATTAAATT AGGTCCTAAT 
GGATCTCCAA GAAGAGCCTG GGGGAAAAGT CCGACAGATT CTGTTCTAAA GATACTTGGA 
GAAGCTGAAC TACAACTTCA GACAGAACTA TTAGAAAATA CAACTATTAG AAGTGAGATT 
TCTCCCGAAG GGGAAAAGTA CAAACCCTTA ATTACTGGAG AAAAAAAAGT ACAATGTATT 
TCACATGAAA TAAACCCATC AGCTATTGTT GATTCTCCTG TTGAGACAAA AAGTCCCGAG 
TTCAGTGAGG CATCTCCACA GATGTCATTG AAACTGGAAG GAAATTTAGA AGAACCTGAT 
GATTTGGAAA CAGAAATTCT ACAAGAGCCA AGTGGAACAA ACAAAGATGA GAGCTTGCCA 
TGCACTATTA CTGATGTGTG GATTAGTGAG GAAAAAGAAA CAAAGGAAAC TCAGTCGGCA 
GATAGGATCA CCATTCAGGA AAATGAAGTT TCTGAAGATG GAGTCTCGAG TACTGTGGAC 
CAACTTAGTG ACATTCATAT AGAGCCTGGA ACCAATGATT CTCAGCACTC TAAATGTGAT 
GTAGATAAGT CTGTGCAACC GGAACCATTT TTCCATAAGG TGGTTCATTC TGAACACTTG 
AACTTAGTCC CTCAAGTTCA ATCAGTTCAG TGTTCACCAG AAGAATCCTT TGCATTTCGA 
TCTCACTCGC ATTTACCACC AAAAAATAAA AACAAGAATT CCTTGCTGAT TGGACTTTCA 
ACTGGTCTGT TTGATGCAAA CAACCCAAAG ATGTTAAGGA CATGTTCACT TCCAGATCTC 
TCAAAGCTGT TCAGAACCCT TATGGATGTT CCCACCGTAG GAGATGTTCG TCAAGACAAT 
CTTGAAATAG ATGAAATTGA AGATGAAAAC ATTAAAGAAG GACCTTCTGA TTCTGAAGAC 
ATTGTGTTTG AAGAAACTGA CACAGATTTA CAAGAGCTGC AGGCCTCGAT GGAACAGTTA 
CTTAGGGAAC AACCTGGTGA AGAATACAGT GAAGAAGAAG AGTCAGTCTT GAAGAACAGT 
GATGTGGAGC CAACTGCAAA TGGGACAGAT GTGGCAGATG AAGATGACAA TCCCAGCAGT 
GAAAGTGCCC TGAACGAAGA ATGGCACTCA GATAACAGTG ATGGTGAAAT TGCTAGTGAA 
TGTGAATGCG ATAGTGTCTT TAACCATTTA GAGGAACTGA GACTTCATCT GGAGCAGGAA 
ATGGGCTTTG AAAAATTCTT TGAGGTTTAT GAGAAAATAA AGGCTATTCA TGAAGATGAA 
GATGAAAATA TTGAAATTTG TTCAAAAATA GTTCAAAATA TTTTGGGAAA TGAACATCAG 
CATCTTTATG CCAAGATTCT TCATTTAGTC ATGGCAGATG GAGCCTACCA AGAAGATAAT 
GATGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( NM_001199400.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001186329.1 Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu11869
Clone ID Related Accession (Same CDS sequence)	XM_011532004.1 , NM_001199400.2 , NM_001199400.1
Accession Version	NM_001199400.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek1 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA697159.1, AB067488.1, CR936672.1, CR933642.1, AK025658.1, AI984346.1 and AC116615.5. On Jun 2, 2019 this sequence version replaced NM_001199400.1. Transcript Variant: This variant (5) differs in the 5' UTR and lacks two alternate in-frame exons compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CR936672.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTGGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG GCAAAAAGGG 
CAGCTAGCTG TAGAAAGAGC TAAACAAGTA GAAGAGTTCC TGCAGCGAAA ACGGGAAGCT 
ATGCAGAATA AAGCTCGAGC CGAAGGACAT ATGGTTTATC TGGCAAGACT GAGGCAAATA 
AGACTACAGA ATTTCAATGA GCGCCAACAG ATTAAAGCCA AACTTCGTGG TGAAAAGAAA 
GAAGCTAATC ATTCTGAAGG ACAAGAAGGA AGTGAAGAGG CTGACATGAG GCGCAAAAAA 
ATCGAATCAC TGAAGGCCCA TGCAAATGCA CGTGCTGCTG TACTAAAAGA ACAACTAGAA 
CGAAAGAGAA AGGAGGCTTA TGAGAGAGAA AAAAAAGTGT GGGAAGAGCA TTTGGTGGCT 
AAAGGAGTTA AGAGTTCTGA TGTTTCTCCA CCTTTGGGAC AGCATGAAAC AGGTGGCTCT 
CCATCAAAGC AACAGATGAG ATCTGTTATT TCTGTAACTT CAGCTTTGAA AGAAGTTGGC 
GTGGACAGTA GTTTAACTGA TACCCGGGAA ACTTCAGAAG AGATGCAAAA GACCAACAAT 
GCTATTTCAA GTAAGCGAGA AATACTTCGT AGATTAAATG AAAATCTTAA AGCTCAAGAA 
GATGAAAAAG GAAAGCAGAA TCTCTCTGAT ACTTTTGAGA TAAATGTTCA TGAAGATGCC 
AAAGAGCATG AAAAAGAAAA ATCAGTTTCA TCTGATCGCA AGAAGTGGGA GGCAGGAGGT 
CAACTTGTGA TTCCTCTGGA TGAGTTAACA CTAGATACAT CCTTCTCTAC AACTGAAAGA 
CATACAGTGG GAGAAGTTAT TAAATTAGGT CCTAATGGAT CTCCAAGAAG AGCCTGGGGG 
AAAAGTCCGA CAGATTCTGT TCTAAAGATA CTTGGAGAAG CTGAACTACA ACTTCAGACA 
GAACTATTAG AAAATACAAC TATTAGAAGT GAGATTTCTC CCGAAGGGGA AAAGTACAAA 
CCCTTAATTA CTGGAGAAAA AAAAGTACAA TGTATTTCAC ATGAAATAAA CCCATCAGCT 
ATTGTTGATT CTCCTGTTGA GACAAAAAGT CCCGAGTTCA GTGAGGCATC TCCACAGATG 
TCATTGAAAC TGGAAGGAAA TTTAGAAGAA CCTGATGATT TGGAAACAGA AATTCTACAA 
GAGCCAAGTG GAACAAACAA AGATGAGAGC TTGCCATGCA CTATTACTGA TGTGTGGATT 
AGTGAGGAAA AAGAAACAAA GGAAACTCAG TCGGCAGATA GGATCACCAT TCAGGAAAAT 
GAAGTTTCTG AAGATGGAGT CTCGAGTACT GTGGACCAAC TTAGTGACAT TCATATAGAG 
CCTGGAACCA ATGATTCTCA GCACTCTAAA TGTGATGTAG ATAAGTCTGT GCAACCGGAA 
CCATTTTTCC ATAAGGTGGT TCATTCTGAA CACTTGAACT TAGTCCCTCA AGTTCAATCA 
GTTCAGTGTT CACCAGAAGA ATCCTTTGCA TTTCGATCTC ACTCGCATTT ACCACCAAAA 
AATAAAAACA AGAATTCCTT GCTGATTGGA CTTTCAACTG GTCTGTTTGA TGCAAACAAC 
CCAAAGATGT TAAGGACATG TTCACTTCCA GATCTCTCAA AGCTGTTCAG AACCCTTATG 
GATGTTCCCA CCGTAGGAGA TGTTCGTCAA GACAATCTTG AAATAGATGA AATTGAAGAT 
GAAAACATTA AAGAAGGACC TTCTGATTCT GAAGACATTG TGTTTGAAGA AACTGACACA 
GATTTACAAG AGCTGCAGGC CTCGATGGAA CAGTTACTTA GGGAACAACC TGGTGAAGAA 
TACAGTGAAG AAGAAGAGTC AGTCTTGAAG AACAGTGATG TGGAGCCAAC TGCAAATGGG 
ACAGATGTGG CAGATGAAGA TGACAATCCC AGCAGTGAAA GTGCCCTGAA CGAAGAATGG 
CACTCAGATA ACAGTGATGG TGAAATTGCT AGTGAATGTG AATGCGATAG TGTCTTTAAC 
CATTTAGAGG AACTGAGACT TCATCTGGAG CAGGAAATGG GCTTTGAAAA ATTCTTTGAG 
GTTTATGAGA AAATAAAGGC TATTCATGAA GATGAAGATG AAAATATTGA AATTTGTTCA 
AAAATAGTTC AAAATATTTT GGGAAATGAA CATCAGCATC TTTATGCCAA GATTCTTCAT 
TTAGTCATGG CAGATGGAGC CTACCAAGAA GATAATGATG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186329.1
CDS	613..4257
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKPLQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMVYLARLRQIRLQNFNERQQIKAKLRGEKKEANHSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQNLSDTFEINVHEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLGPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSEISPEGEKYKPLITGEKKVQCISHEINPSAIVDSPVETKSPEFSEASPQMSLKLEGNLEEPDDLETEILQEPSGTNKDESLPCTITDVWISEEKETKETQSADRITIQENEVSEDGVSSTVDQLSDIHIEPGTNDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGEIASECECDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	NM_001199400.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199400.2

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTGGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG GCAAAAAGGG 
CAGCTAGCTG TAGAAAGAGC TAAACAAGTA GAAGAGTTCC TGCAGCGAAA ACGGGAAGCT 
ATGCAGAATA AAGCTCGAGC CGAAGGACAT ATGGTTTATC TGGCAAGACT GAGGCAAATA 
AGACTACAGA ATTTCAATGA GCGCCAACAG ATTAAAGCCA AACTTCGTGG TGAAAAGAAA 
GAAGCTAATC ATTCTGAAGG ACAAGAAGGA AGTGAAGAGG CTGACATGAG GCGCAAAAAA 
ATCGAATCAC TGAAGGCCCA TGCAAATGCA CGTGCTGCTG TACTAAAAGA ACAACTAGAA 
CGAAAGAGAA AGGAGGCTTA TGAGAGAGAA AAAAAAGTGT GGGAAGAGCA TTTGGTGGCT 
AAAGGAGTTA AGAGTTCTGA TGTTTCTCCA CCTTTGGGAC AGCATGAAAC AGGTGGCTCT 
CCATCAAAGC AACAGATGAG ATCTGTTATT TCTGTAACTT CAGCTTTGAA AGAAGTTGGC 
GTGGACAGTA GTTTAACTGA TACCCGGGAA ACTTCAGAAG AGATGCAAAA GACCAACAAT 
GCTATTTCAA GTAAGCGAGA AATACTTCGT AGATTAAATG AAAATCTTAA AGCTCAAGAA 
GATGAAAAAG GAAAGCAGAA TCTCTCTGAT ACTTTTGAGA TAAATGTTCA TGAAGATGCC 
AAAGAGCATG AAAAAGAAAA ATCAGTTTCA TCTGATCGCA AGAAGTGGGA GGCAGGAGGT 
CAACTTGTGA TTCCTCTGGA TGAGTTAACA CTAGATACAT CCTTCTCTAC AACTGAAAGA 
CATACAGTGG GAGAAGTTAT TAAATTAGGT CCTAATGGAT CTCCAAGAAG AGCCTGGGGG 
AAAAGTCCGA CAGATTCTGT TCTAAAGATA CTTGGAGAAG CTGAACTACA ACTTCAGACA 
GAACTATTAG AAAATACAAC TATTAGAAGT GAGATTTCTC CCGAAGGGGA AAAGTACAAA 
CCCTTAATTA CTGGAGAAAA AAAAGTACAA TGTATTTCAC ATGAAATAAA CCCATCAGCT 
ATTGTTGATT CTCCTGTTGA GACAAAAAGT CCCGAGTTCA GTGAGGCATC TCCACAGATG 
TCATTGAAAC TGGAAGGAAA TTTAGAAGAA CCTGATGATT TGGAAACAGA AATTCTACAA 
GAGCCAAGTG GAACAAACAA AGATGAGAGC TTGCCATGCA CTATTACTGA TGTGTGGATT 
AGTGAGGAAA AAGAAACAAA GGAAACTCAG TCGGCAGATA GGATCACCAT TCAGGAAAAT 
GAAGTTTCTG AAGATGGAGT CTCGAGTACT GTGGACCAAC TTAGTGACAT TCATATAGAG 
CCTGGAACCA ATGATTCTCA GCACTCTAAA TGTGATGTAG ATAAGTCTGT GCAACCGGAA 
CCATTTTTCC ATAAGGTGGT TCATTCTGAA CACTTGAACT TAGTCCCTCA AGTTCAATCA 
GTTCAGTGTT CACCAGAAGA ATCCTTTGCA TTTCGATCTC ACTCGCATTT ACCACCAAAA 
AATAAAAACA AGAATTCCTT GCTGATTGGA CTTTCAACTG GTCTGTTTGA TGCAAACAAC 
CCAAAGATGT TAAGGACATG TTCACTTCCA GATCTCTCAA AGCTGTTCAG AACCCTTATG 
GATGTTCCCA CCGTAGGAGA TGTTCGTCAA GACAATCTTG AAATAGATGA AATTGAAGAT 
GAAAACATTA AAGAAGGACC TTCTGATTCT GAAGACATTG TGTTTGAAGA AACTGACACA 
GATTTACAAG AGCTGCAGGC CTCGATGGAA CAGTTACTTA GGGAACAACC TGGTGAAGAA 
TACAGTGAAG AAGAAGAGTC AGTCTTGAAG AACAGTGATG TGGAGCCAAC TGCAAATGGG 
ACAGATGTGG CAGATGAAGA TGACAATCCC AGCAGTGAAA GTGCCCTGAA CGAAGAATGG 
CACTCAGATA ACAGTGATGG TGAAATTGCT AGTGAATGTG AATGCGATAG TGTCTTTAAC 
CATTTAGAGG AACTGAGACT TCATCTGGAG CAGGAAATGG GCTTTGAAAA ATTCTTTGAG 
GTTTATGAGA AAATAAAGGC TATTCATGAA GATGAAGATG AAAATATTGA AATTTGTTCA 
AAAATAGTTC AAAATATTTT GGGAAATGAA CATCAGCATC TTTATGCCAA GATTCTTCAT 
TTAGTCATGG CAGATGGAGC CTACCAAGAA GATAATGATG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( NM_001199400.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001186329.1 Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu11869
Clone ID Related Accession (Same CDS sequence)	XM_011532004.1 , NM_001199400.2 , NM_001199400.1
Accession Version	NM_001199400.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek1 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA697159.1, AB067488.1, CR936672.1, CR933642.1, AK025658.1 and AI984346.1. On Aug 31, 2013 this sequence version replaced XM_005263028.1. Transcript Variant: This variant (5) differs in the 5' UTR and lacks two alternate in-frame exons compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CR936672.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTGGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG GCAAAAAGGG 
CAGCTAGCTG TAGAAAGAGC TAAACAAGTA GAAGAGTTCC TGCAGCGAAA ACGGGAAGCT 
ATGCAGAATA AAGCTCGAGC CGAAGGACAT ATGGTTTATC TGGCAAGACT GAGGCAAATA 
AGACTACAGA ATTTCAATGA GCGCCAACAG ATTAAAGCCA AACTTCGTGG TGAAAAGAAA 
GAAGCTAATC ATTCTGAAGG ACAAGAAGGA AGTGAAGAGG CTGACATGAG GCGCAAAAAA 
ATCGAATCAC TGAAGGCCCA TGCAAATGCA CGTGCTGCTG TACTAAAAGA ACAACTAGAA 
CGAAAGAGAA AGGAGGCTTA TGAGAGAGAA AAAAAAGTGT GGGAAGAGCA TTTGGTGGCT 
AAAGGAGTTA AGAGTTCTGA TGTTTCTCCA CCTTTGGGAC AGCATGAAAC AGGTGGCTCT 
CCATCAAAGC AACAGATGAG ATCTGTTATT TCTGTAACTT CAGCTTTGAA AGAAGTTGGC 
GTGGACAGTA GTTTAACTGA TACCCGGGAA ACTTCAGAAG AGATGCAAAA GACCAACAAT 
GCTATTTCAA GTAAGCGAGA AATACTTCGT AGATTAAATG AAAATCTTAA AGCTCAAGAA 
GATGAAAAAG GAAAGCAGAA TCTCTCTGAT ACTTTTGAGA TAAATGTTCA TGAAGATGCC 
AAAGAGCATG AAAAAGAAAA ATCAGTTTCA TCTGATCGCA AGAAGTGGGA GGCAGGAGGT 
CAACTTGTGA TTCCTCTGGA TGAGTTAACA CTAGATACAT CCTTCTCTAC AACTGAAAGA 
CATACAGTGG GAGAAGTTAT TAAATTAGGT CCTAATGGAT CTCCAAGAAG AGCCTGGGGG 
AAAAGTCCGA CAGATTCTGT TCTAAAGATA CTTGGAGAAG CTGAACTACA ACTTCAGACA 
GAACTATTAG AAAATACAAC TATTAGAAGT GAGATTTCTC CCGAAGGGGA AAAGTACAAA 
CCCTTAATTA CTGGAGAAAA AAAAGTACAA TGTATTTCAC ATGAAATAAA CCCATCAGCT 
ATTGTTGATT CTCCTGTTGA GACAAAAAGT CCCGAGTTCA GTGAGGCATC TCCACAGATG 
TCATTGAAAC TGGAAGGAAA TTTAGAAGAA CCTGATGATT TGGAAACAGA AATTCTACAA 
GAGCCAAGTG GAACAAACAA AGATGAGAGC TTGCCATGCA CTATTACTGA TGTGTGGATT 
AGTGAGGAAA AAGAAACAAA GGAAACTCAG TCGGCAGATA GGATCACCAT TCAGGAAAAT 
GAAGTTTCTG AAGATGGAGT CTCGAGTACT GTGGACCAAC TTAGTGACAT TCATATAGAG 
CCTGGAACCA ATGATTCTCA GCACTCTAAA TGTGATGTAG ATAAGTCTGT GCAACCGGAA 
CCATTTTTCC ATAAGGTGGT TCATTCTGAA CACTTGAACT TAGTCCCTCA AGTTCAATCA 
GTTCAGTGTT CACCAGAAGA ATCCTTTGCA TTTCGATCTC ACTCGCATTT ACCACCAAAA 
AATAAAAACA AGAATTCCTT GCTGATTGGA CTTTCAACTG GTCTGTTTGA TGCAAACAAC 
CCAAAGATGT TAAGGACATG TTCACTTCCA GATCTCTCAA AGCTGTTCAG AACCCTTATG 
GATGTTCCCA CCGTAGGAGA TGTTCGTCAA GACAATCTTG AAATAGATGA AATTGAAGAT 
GAAAACATTA AAGAAGGACC TTCTGATTCT GAAGACATTG TGTTTGAAGA AACTGACACA 
GATTTACAAG AGCTGCAGGC CTCGATGGAA CAGTTACTTA GGGAACAACC TGGTGAAGAA 
TACAGTGAAG AAGAAGAGTC AGTCTTGAAG AACAGTGATG TGGAGCCAAC TGCAAATGGG 
ACAGATGTGG CAGATGAAGA TGACAATCCC AGCAGTGAAA GTGCCCTGAA CGAAGAATGG 
CACTCAGATA ACAGTGATGG TGAAATTGCT AGTGAATGTG AATGCGATAG TGTCTTTAAC 
CATTTAGAGG AACTGAGACT TCATCTGGAG CAGGAAATGG GCTTTGAAAA ATTCTTTGAG 
GTTTATGAGA AAATAAAGGC TATTCATGAA GATGAAGATG AAAATATTGA AATTTGTTCA 
AAAATAGTTC AAAATATTTT GGGAAATGAA CATCAGCATC TTTATGCCAA GATTCTTCAT 
TTAGTCATGG CAGATGGAGC CTACCAAGAA GATAATGATG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186329.1
CDS	657..4301
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKPLQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMVYLARLRQIRLQNFNERQQIKAKLRGEKKEANHSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQNLSDTFEINVHEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLGPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSEISPEGEKYKPLITGEKKVQCISHEINPSAIVDSPVETKSPEFSEASPQMSLKLEGNLEEPDDLETEILQEPSGTNKDESLPCTITDVWISEEKETKETQSADRITIQENEVSEDGVSSTVDQLSDIHIEPGTNDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGEIASECECDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	NM_001199400.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199400.1

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTGGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG GCAAAAAGGG 
CAGCTAGCTG TAGAAAGAGC TAAACAAGTA GAAGAGTTCC TGCAGCGAAA ACGGGAAGCT 
ATGCAGAATA AAGCTCGAGC CGAAGGACAT ATGGTTTATC TGGCAAGACT GAGGCAAATA 
AGACTACAGA ATTTCAATGA GCGCCAACAG ATTAAAGCCA AACTTCGTGG TGAAAAGAAA 
GAAGCTAATC ATTCTGAAGG ACAAGAAGGA AGTGAAGAGG CTGACATGAG GCGCAAAAAA 
ATCGAATCAC TGAAGGCCCA TGCAAATGCA CGTGCTGCTG TACTAAAAGA ACAACTAGAA 
CGAAAGAGAA AGGAGGCTTA TGAGAGAGAA AAAAAAGTGT GGGAAGAGCA TTTGGTGGCT 
AAAGGAGTTA AGAGTTCTGA TGTTTCTCCA CCTTTGGGAC AGCATGAAAC AGGTGGCTCT 
CCATCAAAGC AACAGATGAG ATCTGTTATT TCTGTAACTT CAGCTTTGAA AGAAGTTGGC 
GTGGACAGTA GTTTAACTGA TACCCGGGAA ACTTCAGAAG AGATGCAAAA GACCAACAAT 
GCTATTTCAA GTAAGCGAGA AATACTTCGT AGATTAAATG AAAATCTTAA AGCTCAAGAA 
GATGAAAAAG GAAAGCAGAA TCTCTCTGAT ACTTTTGAGA TAAATGTTCA TGAAGATGCC 
AAAGAGCATG AAAAAGAAAA ATCAGTTTCA TCTGATCGCA AGAAGTGGGA GGCAGGAGGT 
CAACTTGTGA TTCCTCTGGA TGAGTTAACA CTAGATACAT CCTTCTCTAC AACTGAAAGA 
CATACAGTGG GAGAAGTTAT TAAATTAGGT CCTAATGGAT CTCCAAGAAG AGCCTGGGGG 
AAAAGTCCGA CAGATTCTGT TCTAAAGATA CTTGGAGAAG CTGAACTACA ACTTCAGACA 
GAACTATTAG AAAATACAAC TATTAGAAGT GAGATTTCTC CCGAAGGGGA AAAGTACAAA 
CCCTTAATTA CTGGAGAAAA AAAAGTACAA TGTATTTCAC ATGAAATAAA CCCATCAGCT 
ATTGTTGATT CTCCTGTTGA GACAAAAAGT CCCGAGTTCA GTGAGGCATC TCCACAGATG 
TCATTGAAAC TGGAAGGAAA TTTAGAAGAA CCTGATGATT TGGAAACAGA AATTCTACAA 
GAGCCAAGTG GAACAAACAA AGATGAGAGC TTGCCATGCA CTATTACTGA TGTGTGGATT 
AGTGAGGAAA AAGAAACAAA GGAAACTCAG TCGGCAGATA GGATCACCAT TCAGGAAAAT 
GAAGTTTCTG AAGATGGAGT CTCGAGTACT GTGGACCAAC TTAGTGACAT TCATATAGAG 
CCTGGAACCA ATGATTCTCA GCACTCTAAA TGTGATGTAG ATAAGTCTGT GCAACCGGAA 
CCATTTTTCC ATAAGGTGGT TCATTCTGAA CACTTGAACT TAGTCCCTCA AGTTCAATCA 
GTTCAGTGTT CACCAGAAGA ATCCTTTGCA TTTCGATCTC ACTCGCATTT ACCACCAAAA 
AATAAAAACA AGAATTCCTT GCTGATTGGA CTTTCAACTG GTCTGTTTGA TGCAAACAAC 
CCAAAGATGT TAAGGACATG TTCACTTCCA GATCTCTCAA AGCTGTTCAG AACCCTTATG 
GATGTTCCCA CCGTAGGAGA TGTTCGTCAA GACAATCTTG AAATAGATGA AATTGAAGAT 
GAAAACATTA AAGAAGGACC TTCTGATTCT GAAGACATTG TGTTTGAAGA AACTGACACA 
GATTTACAAG AGCTGCAGGC CTCGATGGAA CAGTTACTTA GGGAACAACC TGGTGAAGAA 
TACAGTGAAG AAGAAGAGTC AGTCTTGAAG AACAGTGATG TGGAGCCAAC TGCAAATGGG 
ACAGATGTGG CAGATGAAGA TGACAATCCC AGCAGTGAAA GTGCCCTGAA CGAAGAATGG 
CACTCAGATA ACAGTGATGG TGAAATTGCT AGTGAATGTG AATGCGATAG TGTCTTTAAC 
CATTTAGAGG AACTGAGACT TCATCTGGAG CAGGAAATGG GCTTTGAAAA ATTCTTTGAG 
GTTTATGAGA AAATAAAGGC TATTCATGAA GATGAAGATG AAAATATTGA AATTTGTTCA 
AAAATAGTTC AAAATATTTT GGGAAATGAA CATCAGCATC TTTATGCCAA GATTCTTCAT 
TTAGTCATGG CAGATGGAGC CTACCAAGAA GATAATGATG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_011532004.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530306.1 Homo sapiens NIMA related kinase 1 (NEK1), transcript variant X3, mRNA.

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CloneID	OHu11869
Clone ID Related Accession (Same CDS sequence)	XM_011532004.1 , NM_001199400.2 , NM_001199400.1
Accession Version	XM_011532004.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTGGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG GCAAAAAGGG 
CAGCTAGCTG TAGAAAGAGC TAAACAAGTA GAAGAGTTCC TGCAGCGAAA ACGGGAAGCT 
ATGCAGAATA AAGCTCGAGC CGAAGGACAT ATGGTTTATC TGGCAAGACT GAGGCAAATA 
AGACTACAGA ATTTCAATGA GCGCCAACAG ATTAAAGCCA AACTTCGTGG TGAAAAGAAA 
GAAGCTAATC ATTCTGAAGG ACAAGAAGGA AGTGAAGAGG CTGACATGAG GCGCAAAAAA 
ATCGAATCAC TGAAGGCCCA TGCAAATGCA CGTGCTGCTG TACTAAAAGA ACAACTAGAA 
CGAAAGAGAA AGGAGGCTTA TGAGAGAGAA AAAAAAGTGT GGGAAGAGCA TTTGGTGGCT 
AAAGGAGTTA AGAGTTCTGA TGTTTCTCCA CCTTTGGGAC AGCATGAAAC AGGTGGCTCT 
CCATCAAAGC AACAGATGAG ATCTGTTATT TCTGTAACTT CAGCTTTGAA AGAAGTTGGC 
GTGGACAGTA GTTTAACTGA TACCCGGGAA ACTTCAGAAG AGATGCAAAA GACCAACAAT 
GCTATTTCAA GTAAGCGAGA AATACTTCGT AGATTAAATG AAAATCTTAA AGCTCAAGAA 
GATGAAAAAG GAAAGCAGAA TCTCTCTGAT ACTTTTGAGA TAAATGTTCA TGAAGATGCC 
AAAGAGCATG AAAAAGAAAA ATCAGTTTCA TCTGATCGCA AGAAGTGGGA GGCAGGAGGT 
CAACTTGTGA TTCCTCTGGA TGAGTTAACA CTAGATACAT CCTTCTCTAC AACTGAAAGA 
CATACAGTGG GAGAAGTTAT TAAATTAGGT CCTAATGGAT CTCCAAGAAG AGCCTGGGGG 
AAAAGTCCGA CAGATTCTGT TCTAAAGATA CTTGGAGAAG CTGAACTACA ACTTCAGACA 
GAACTATTAG AAAATACAAC TATTAGAAGT GAGATTTCTC CCGAAGGGGA AAAGTACAAA 
CCCTTAATTA CTGGAGAAAA AAAAGTACAA TGTATTTCAC ATGAAATAAA CCCATCAGCT 
ATTGTTGATT CTCCTGTTGA GACAAAAAGT CCCGAGTTCA GTGAGGCATC TCCACAGATG 
TCATTGAAAC TGGAAGGAAA TTTAGAAGAA CCTGATGATT TGGAAACAGA AATTCTACAA 
GAGCCAAGTG GAACAAACAA AGATGAGAGC TTGCCATGCA CTATTACTGA TGTGTGGATT 
AGTGAGGAAA AAGAAACAAA GGAAACTCAG TCGGCAGATA GGATCACCAT TCAGGAAAAT 
GAAGTTTCTG AAGATGGAGT CTCGAGTACT GTGGACCAAC TTAGTGACAT TCATATAGAG 
CCTGGAACCA ATGATTCTCA GCACTCTAAA TGTGATGTAG ATAAGTCTGT GCAACCGGAA 
CCATTTTTCC ATAAGGTGGT TCATTCTGAA CACTTGAACT TAGTCCCTCA AGTTCAATCA 
GTTCAGTGTT CACCAGAAGA ATCCTTTGCA TTTCGATCTC ACTCGCATTT ACCACCAAAA 
AATAAAAACA AGAATTCCTT GCTGATTGGA CTTTCAACTG GTCTGTTTGA TGCAAACAAC 
CCAAAGATGT TAAGGACATG TTCACTTCCA GATCTCTCAA AGCTGTTCAG AACCCTTATG 
GATGTTCCCA CCGTAGGAGA TGTTCGTCAA GACAATCTTG AAATAGATGA AATTGAAGAT 
GAAAACATTA AAGAAGGACC TTCTGATTCT GAAGACATTG TGTTTGAAGA AACTGACACA 
GATTTACAAG AGCTGCAGGC CTCGATGGAA CAGTTACTTA GGGAACAACC TGGTGAAGAA 
TACAGTGAAG AAGAAGAGTC AGTCTTGAAG AACAGTGATG TGGAGCCAAC TGCAAATGGG 
ACAGATGTGG CAGATGAAGA TGACAATCCC AGCAGTGAAA GTGCCCTGAA CGAAGAATGG 
CACTCAGATA ACAGTGATGG TGAAATTGCT AGTGAATGTG AATGCGATAG TGTCTTTAAC 
CATTTAGAGG AACTGAGACT TCATCTGGAG CAGGAAATGG GCTTTGAAAA ATTCTTTGAG 
GTTTATGAGA AAATAAAGGC TATTCATGAA GATGAAGATG AAAATATTGA AATTTGTTCA 
AAAATAGTTC AAAATATTTT GGGAAATGAA CATCAGCATC TTTATGCCAA GATTCTTCAT 
TTAGTCATGG CAGATGGAGC CTACCAAGAA GATAATGATG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530306.1
CDS	581..4225
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKPLQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMVYLARLRQIRLQNFNERQQIKAKLRGEKKEANHSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQNLSDTFEINVHEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLGPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSEISPEGEKYKPLITGEKKVQCISHEINPSAIVDSPVETKSPEFSEASPQMSLKLEGNLEEPDDLETEILQEPSGTNKDESLPCTITDVWISEEKETKETQSADRITIQENEVSEDGVSSTVDQLSDIHIEPGTNDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGEIASECECDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_011532004.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532004.1

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTGGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG GCAAAAAGGG 
CAGCTAGCTG TAGAAAGAGC TAAACAAGTA GAAGAGTTCC TGCAGCGAAA ACGGGAAGCT 
ATGCAGAATA AAGCTCGAGC CGAAGGACAT ATGGTTTATC TGGCAAGACT GAGGCAAATA 
AGACTACAGA ATTTCAATGA GCGCCAACAG ATTAAAGCCA AACTTCGTGG TGAAAAGAAA 
GAAGCTAATC ATTCTGAAGG ACAAGAAGGA AGTGAAGAGG CTGACATGAG GCGCAAAAAA 
ATCGAATCAC TGAAGGCCCA TGCAAATGCA CGTGCTGCTG TACTAAAAGA ACAACTAGAA 
CGAAAGAGAA AGGAGGCTTA TGAGAGAGAA AAAAAAGTGT GGGAAGAGCA TTTGGTGGCT 
AAAGGAGTTA AGAGTTCTGA TGTTTCTCCA CCTTTGGGAC AGCATGAAAC AGGTGGCTCT 
CCATCAAAGC AACAGATGAG ATCTGTTATT TCTGTAACTT CAGCTTTGAA AGAAGTTGGC 
GTGGACAGTA GTTTAACTGA TACCCGGGAA ACTTCAGAAG AGATGCAAAA GACCAACAAT 
GCTATTTCAA GTAAGCGAGA AATACTTCGT AGATTAAATG AAAATCTTAA AGCTCAAGAA 
GATGAAAAAG GAAAGCAGAA TCTCTCTGAT ACTTTTGAGA TAAATGTTCA TGAAGATGCC 
AAAGAGCATG AAAAAGAAAA ATCAGTTTCA TCTGATCGCA AGAAGTGGGA GGCAGGAGGT 
CAACTTGTGA TTCCTCTGGA TGAGTTAACA CTAGATACAT CCTTCTCTAC AACTGAAAGA 
CATACAGTGG GAGAAGTTAT TAAATTAGGT CCTAATGGAT CTCCAAGAAG AGCCTGGGGG 
AAAAGTCCGA CAGATTCTGT TCTAAAGATA CTTGGAGAAG CTGAACTACA ACTTCAGACA 
GAACTATTAG AAAATACAAC TATTAGAAGT GAGATTTCTC CCGAAGGGGA AAAGTACAAA 
CCCTTAATTA CTGGAGAAAA AAAAGTACAA TGTATTTCAC ATGAAATAAA CCCATCAGCT 
ATTGTTGATT CTCCTGTTGA GACAAAAAGT CCCGAGTTCA GTGAGGCATC TCCACAGATG 
TCATTGAAAC TGGAAGGAAA TTTAGAAGAA CCTGATGATT TGGAAACAGA AATTCTACAA 
GAGCCAAGTG GAACAAACAA AGATGAGAGC TTGCCATGCA CTATTACTGA TGTGTGGATT 
AGTGAGGAAA AAGAAACAAA GGAAACTCAG TCGGCAGATA GGATCACCAT TCAGGAAAAT 
GAAGTTTCTG AAGATGGAGT CTCGAGTACT GTGGACCAAC TTAGTGACAT TCATATAGAG 
CCTGGAACCA ATGATTCTCA GCACTCTAAA TGTGATGTAG ATAAGTCTGT GCAACCGGAA 
CCATTTTTCC ATAAGGTGGT TCATTCTGAA CACTTGAACT TAGTCCCTCA AGTTCAATCA 
GTTCAGTGTT CACCAGAAGA ATCCTTTGCA TTTCGATCTC ACTCGCATTT ACCACCAAAA 
AATAAAAACA AGAATTCCTT GCTGATTGGA CTTTCAACTG GTCTGTTTGA TGCAAACAAC 
CCAAAGATGT TAAGGACATG TTCACTTCCA GATCTCTCAA AGCTGTTCAG AACCCTTATG 
GATGTTCCCA CCGTAGGAGA TGTTCGTCAA GACAATCTTG AAATAGATGA AATTGAAGAT 
GAAAACATTA AAGAAGGACC TTCTGATTCT GAAGACATTG TGTTTGAAGA AACTGACACA 
GATTTACAAG AGCTGCAGGC CTCGATGGAA CAGTTACTTA GGGAACAACC TGGTGAAGAA 
TACAGTGAAG AAGAAGAGTC AGTCTTGAAG AACAGTGATG TGGAGCCAAC TGCAAATGGG 
ACAGATGTGG CAGATGAAGA TGACAATCCC AGCAGTGAAA GTGCCCTGAA CGAAGAATGG 
CACTCAGATA ACAGTGATGG TGAAATTGCT AGTGAATGTG AATGCGATAG TGTCTTTAAC 
CATTTAGAGG AACTGAGACT TCATCTGGAG CAGGAAATGG GCTTTGAAAA ATTCTTTGAG 
GTTTATGAGA AAATAAAGGC TATTCATGAA GATGAAGATG AAAATATTGA AATTTGTTCA 
AAAATAGTTC AAAATATTTT GGGAAATGAA CATCAGCATC TTTATGCCAA GATTCTTCAT 
TTAGTCATGG CAGATGGAGC CTACCAAGAA GATAATGATG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEK1 ( XM_006714228.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714291.1 Homo sapiens NIMA related kinase 1 (NEK1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu38950
Clone ID Related Accession (Same CDS sequence)	XM_006714228.1
Accession Version	XM_006714228.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3684bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase Nek1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTGGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG AGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT TCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGGAAT CCTGCAAAAC 
CTGGCAGCTA TGTATGGAGG CAGGCCCAGC TCTTCAAGAG GAGGGAAGCC AAGAAACAAA 
GAGGAAGAGG TTTATCTGGC AAGACTGAGG CAAATAAGAC TACAGAATTT CAATGAGCGC 
CAACAGATTA AAGCCAAACT TCGTGGTGAA AAGAAAGAAG CTAATCATTC TGAAGGACAA 
GAAGGAAGTG AAGAGGCTGA CATGAGGCGC AAAAAAATCG AATCACTGAA GGCCCATGCA 
AATGCACGTG CTGCTGTACT AAAAGAACAA CTAGAACGAA AGAGAAAGGA GGCTTATGAG 
AGAGAAAAAA AAGTGTGGGA AGAGCATTTG GTGGCTAAAG GAGTTAAGAG TTCTGATGTT 
TCTCCACCTT TGGGACAGCA TGAAACAGGT GGCTCTCCAT CAAAGCAACA GATGAGATCT 
GTTATTTCTG TAACTTCAGC TTTGAAAGAA GTTGGCGTGG ACAGTAGTTT AACTGATACC 
CGGGAAACTT CAGAAGAGAT GCAAAAGACC AACAATGCTA TTTCAAGTAA GCGAGAAATA 
CTTCGTAGAT TAAATGAAAA TCTTAAAGCT CAAGAAGATG AAAAAGGAAA GCAGAATCTC 
TCTGATACTT TTGAGATAAA TGTTCATGAA GATGCCAAAG AGCATGAAAA AGAAAAATCA 
GTTTCATCTG ATCGCAAGAA GTGGGAGGCA GGAGGTCAAC TTGTGATTCC TCTGGATGAG 
TTAACACTAG ATACATCCTT CTCTACAACT GAAAGACATA CAGTGGGAGA AGTTATTAAA 
TTAGGTCCTA ATGGATCTCC AAGAAGAGCC TGGGGGAAAA GTCCGACAGA TTCTGTTCTA 
AAGATACTTG GAGAAGCTGA ACTACAACTT CAGACAGAAC TATTAGAAAA TACAACTATT 
AGAAGTGAAC CTGATGATTT GGAAACAGAA ATTCTACAAG AGCCAAGTGG AACAAACAAA 
GATGAGAGCT TGCCATGCAC TATTACTGAT GTGTGGATTA GTGAGGAAAA AGAAACAAAG 
GAAACTCAGT CGGCAGATAG GATCACCATT CAGGAAAATG AAGTTTCTGA AGATGGAGTC 
TCGAGTACTG TGGACCAACT TAGTGACATT CATATAGAGC CTGGAACCAA TGATTCTCAG 
CACTCTAAAT GTGATGTAGA TAAGTCTGTG CAACCGGAAC CATTTTTCCA TAAGGTGGTT 
CATTCTGAAC ACTTGAACTT AGTCCCTCAA GTTCAATCAG TTCAGTGTTC ACCAGAAGAA 
TCCTTTGCAT TTCGATCTCA CTCGCATTTA CCACCAAAAA ATAAAAACAA GAATTCCTTG 
CTGATTGGAC TTTCAACTGG TCTGTTTGAT GCAAACAACC CAAAGATGTT AAGGACATGT 
TCACTTCCAG ATCTCTCAAA GCTGTTCAGA ACCCTTATGG ATGTTCCCAC CGTAGGAGAT 
GTTCGTCAAG ACAATCTTGA AATAGATGAA ATTGAAGATG AAAACATTAA AGAAGGACCT 
TCTGATTCTG AAGACATTGT GTTTGAAGAA ACTGACACAG ATTTACAAGA GCTGCAGGCC 
TCGATGGAAC AGTTACTTAG GGAACAACCT GGTGAAGAAT ACAGTGAAGA AGAAGAGTCA 
GTCTTGAAGA ACAGTGATGT GGAGCCAACT GCAAATGGGA CAGATGTGGC AGATGAAGAT 
GACAATCCCA GCAGTGAAAG TGCCCTGAAC GAAGAATGGC ACTCAGATAA CAGTGATGGT 
GAAATTGCTA GTGAATGTGA ATGCGATAGT GTCTTTAACC ATTTAGAGGA ACTGAGACTT 
CATCTGGAGC AGGAAATGGG CTTTGAAAAA TTCTTTGAGG TTTATGAGAA AATAAAGGCT 
ATTCATGAAG ATGAAGATGA AAATATTGAA ATTTGTTCAA AAATAGTTCA AAATATTTTG 
GGAAATGAAC ATCAGCATCT TTATGCCAAG ATTCTTCATT TAGTCATGGC AGATGGAGCC 
TACCAAGAAG ATAATGATGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714291.1
CDS	581..4264
Translation	MEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKPLQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERGILPGVRPGFPYGAAGHHHFPDADDIRKTLKRLKAVSKQANANRQKGQLAVERAKQVEEFLQRKREAMQNKARAEGHMGILQNLAAMYGGRPSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIKAKLRGEKKEANHSEGQEGSEEADMRRKKIESLKAHANARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDVSPPLGQHETGGSPSKQQMRSVISVTSALKEVGVDSSLTDTRETSEEMQKTNNAISSKREILRRLNENLKAQEDEKGKQNLSDTFEINVHEDAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSTTERHTVGEVIKLGPNGSPRRAWGKSPTDSVLKILGEAELQLQTELLENTTIRSEPDDLETEILQEPSGTNKDESLPCTITDVWISEEKETKETQSADRITIQENEVSEDGVSSTVDQLSDIHIEPGTNDSQHSKCDVDKSVQPEPFFHKVVHSEHLNLVPQVQSVQCSPEESFAFRSHSHLPPKNKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFRTLMDVPTVGDVRQDNLEIDEIEDENIKEGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEYSEEEESVLKNSDVEPTANGTDVADEDDNPSSESALNEEWHSDNSDGEIASECECDSVFNHLEELRLHLEQEMGFEKFFEVYEKIKAIHEDEDENIEICSKIVQNILGNEHQHLYAKILHLVMADGAYQEDNDE

Target ORF information:

RefSeq Version	XM_006714228.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NIMA related kinase 1 (NEK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714228.1

ORF Insert Sequence:

ATGGAGAAGT ATGTTAGACT ACAGAAGATT GGAGAAGGTT CATTTGGAAA AGCCATTCTT 
GTTAAATCTA CAGAAGATGG CAGACAGTAT GTTATCAAGG AAATTAACAT CTCAAGAATG 
TCCAGTAAAG AAAGAGAAGA ATCAAGGAGA GAAGTTGCAG TATTGGCAAA CATGAAGCAT 
CCAAATATTG TCCAGTATAG AGAATCATTT GAAGAAAATG GCTCTCTCTA CATAGTAATG 
GATTACTGTG AGGGAGGGGA TCTGTTTAAG CGAATAAATG CTCAGAAAGG CGTTTTGTTT 
CAAGAGGATC AGATTTTGGA CTGGTTTGTA CAGATATGTT TGGCCCTGAA ACATGTACAT 
GATAGAAAAA TTCTTCATCG AGACATTAAA TCTCAGAACA TATTTTTAAC TAAAGATGGA 
ACAGTACAAC TTGGAGATTT TGGAATTGCT AGAGTTCTTA ATAGTACTGT AGAGCTGGCT 
CGAACTTGCA TAGGGACCCC ATACTACTTG TCACCTGAAA TCTGTGAAAA CAAACCTTAC 
AATAATAAAA GTGACATTTG GGCTCTGGGG TGTGTCCTTT ATGAGCTGTG TACACTTAAA 
CATGCTTTTG AAGCTGGCAG TATGAAAAAC CTGGTACTGA AGATAATATC TGGATCTTTT 
CCACCTGTGT CTTTGCATTA TTCCTATGAT CTCCGCAGTT TGGTGTCTCA GTTATTTAAA 
AGAAATCCTA GGGATAGACC ATCAGTCAAC TCCATATTGG AGAAAGGTTT TATAGCCAAA 
CGCATTGAAA AGTTTCTCTC TCCTCAGCTT ATTGCAGAAG AATTTTGTCT AAAAACATTT 
TCGAAGTTTG GATCACAGCC TATACCAGCT AAAAGACCAG CTTCAGGACA AAACTCGATT 
TCTGTTATGC CTGCTCAGAA AATTACAAAG CCTGCCGCTA AATATGGAAT ACCTTTAGCA 
TATAAGAAAT ATGGAGATAA AAAATTACAC GAAAAGAAAC CACTGCAAAA ACATAAACAG 
GCCCATCAAA CTCCAGAGAA GAGAGTGAAT ACTGGAGAAG AAAGGAGGAA AATATCTGAG 
GAAGCAGCAA GAAAGAGAAG GCTGGAATTT ATTGAAAAAG AAAAGAAACA AAAGGATCAG 
ATTATTAGTT TAATGAAGGC TGAACAAATG AAAAGGCAAG AAAAGGAAAG GTTGGAAAGA 
ATAAATAGGG CCAGGGAACA AGGATGGAGA AATGTGCTAA GTGCTGGTGG AAGTGGTGAA 
GTAAAGGCTC CTTTTCTGGG CAGTGGAGGG ACTATAGCTC CATCATCTTT TTCTTCTCGA 
GGACAGTATG AACATTACCA TGCCATTTTT GACCAAATGC AGCAACAAAG AGCAGAAGAT 
AATGAAGCTA AATGGAAAAG AGAAATATAT GGTCGAGGTC TTCCAGAAAG AGGAATTCTG 
CCTGGAGTTC GTCCAGGATT TCCTTATGGG GCTGCAGGTC ATCACCATTT TCCTGATGCT 
GATGATATTA GAAAAACTTT GAAAAGATTG AAGGCGGTGT CTAAACAAGC CAATGCAAAC 
AGGCAAAAAG GGCAGCTAGC TGTAGAAAGA GCTAAACAAG TAGAAGAGTT CCTGCAGCGA 
AAACGGGAAG CTATGCAGAA TAAAGCTCGA GCCGAAGGAC ATATGGGAAT CCTGCAAAAC 
CTGGCAGCTA TGTATGGAGG CAGGCCCAGC TCTTCAAGAG GAGGGAAGCC AAGAAACAAA 
GAGGAAGAGG TTTATCTGGC AAGACTGAGG CAAATAAGAC TACAGAATTT CAATGAGCGC 
CAACAGATTA AAGCCAAACT TCGTGGTGAA AAGAAAGAAG CTAATCATTC TGAAGGACAA 
GAAGGAAGTG AAGAGGCTGA CATGAGGCGC AAAAAAATCG AATCACTGAA GGCCCATGCA 
AATGCACGTG CTGCTGTACT AAAAGAACAA CTAGAACGAA AGAGAAAGGA GGCTTATGAG 
AGAGAAAAAA AAGTGTGGGA AGAGCATTTG GTGGCTAAAG GAGTTAAGAG TTCTGATGTT 
TCTCCACCTT TGGGACAGCA TGAAACAGGT GGCTCTCCAT CAAAGCAACA GATGAGATCT 
GTTATTTCTG TAACTTCAGC TTTGAAAGAA GTTGGCGTGG ACAGTAGTTT AACTGATACC 
CGGGAAACTT CAGAAGAGAT GCAAAAGACC AACAATGCTA TTTCAAGTAA GCGAGAAATA 
CTTCGTAGAT TAAATGAAAA TCTTAAAGCT CAAGAAGATG AAAAAGGAAA GCAGAATCTC 
TCTGATACTT TTGAGATAAA TGTTCATGAA GATGCCAAAG AGCATGAAAA AGAAAAATCA 
GTTTCATCTG ATCGCAAGAA GTGGGAGGCA GGAGGTCAAC TTGTGATTCC TCTGGATGAG 
TTAACACTAG ATACATCCTT CTCTACAACT GAAAGACATA CAGTGGGAGA AGTTATTAAA 
TTAGGTCCTA ATGGATCTCC AAGAAGAGCC TGGGGGAAAA GTCCGACAGA TTCTGTTCTA 
AAGATACTTG GAGAAGCTGA ACTACAACTT CAGACAGAAC TATTAGAAAA TACAACTATT 
AGAAGTGAAC CTGATGATTT GGAAACAGAA ATTCTACAAG AGCCAAGTGG AACAAACAAA 
GATGAGAGCT TGCCATGCAC TATTACTGAT GTGTGGATTA GTGAGGAAAA AGAAACAAAG 
GAAACTCAGT CGGCAGATAG GATCACCATT CAGGAAAATG AAGTTTCTGA AGATGGAGTC 
TCGAGTACTG TGGACCAACT TAGTGACATT CATATAGAGC CTGGAACCAA TGATTCTCAG 
CACTCTAAAT GTGATGTAGA TAAGTCTGTG CAACCGGAAC CATTTTTCCA TAAGGTGGTT 
CATTCTGAAC ACTTGAACTT AGTCCCTCAA GTTCAATCAG TTCAGTGTTC ACCAGAAGAA 
TCCTTTGCAT TTCGATCTCA CTCGCATTTA CCACCAAAAA ATAAAAACAA GAATTCCTTG 
CTGATTGGAC TTTCAACTGG TCTGTTTGAT GCAAACAACC CAAAGATGTT AAGGACATGT 
TCACTTCCAG ATCTCTCAAA GCTGTTCAGA ACCCTTATGG ATGTTCCCAC CGTAGGAGAT 
GTTCGTCAAG ACAATCTTGA AATAGATGAA ATTGAAGATG AAAACATTAA AGAAGGACCT 
TCTGATTCTG AAGACATTGT GTTTGAAGAA ACTGACACAG ATTTACAAGA GCTGCAGGCC 
TCGATGGAAC AGTTACTTAG GGAACAACCT GGTGAAGAAT ACAGTGAAGA AGAAGAGTCA 
GTCTTGAAGA ACAGTGATGT GGAGCCAACT GCAAATGGGA CAGATGTGGC AGATGAAGAT 
GACAATCCCA GCAGTGAAAG TGCCCTGAAC GAAGAATGGC ACTCAGATAA CAGTGATGGT 
GAAATTGCTA GTGAATGTGA ATGCGATAGT GTCTTTAACC ATTTAGAGGA ACTGAGACTT 
CATCTGGAGC AGGAAATGGG CTTTGAAAAA TTCTTTGAGG TTTATGAGAA AATAAAGGCT 
ATTCATGAAG ATGAAGATGA AAATATTGAA ATTTGTTCAA AAATAGTTCA AAATATTTTG 
GGAAATGAAC ATCAGCATCT TTATGCCAAG ATTCTTCATT TAGTCATGGC AGATGGAGCC 
TACCAAGAAG ATAATGATGA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NEK1
Entrez Gene ID	4750
Full Name	NIMA related kinase 1
Synonyms	NY-REN-55,SRPS2,SRPS2A,SRTD6
General protein information	Preferred Names NIMA related kinase 1 Names serine/threonine-protein kinase Nek1 NIMA (never in mitosis gene a)-related kinase 1 never in mitosis A-related kinase 1 nimA-related protein kinase 1 protein-serine/threonine kinase renal carcinoma antigen NY-REN-55
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q33
Summary	The protein encoded by this gene is a serine/threonine kinase involved in cell cycle regulation. The encoded protein is found in a centrosomal complex with FEZ1, a neuronal protein that plays a role in axonal development. Defects in this gene are a cause of polycystic kidney disease (PKD). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2010].
Disorder MIM:	604588
Disorder Html:	Short-rib thoracic dysplasia 6 with or without polydactyly, 263520 (3)

Bos taurus (cattle)	NEK1	XP_003586402.2
Rattus norvegicus (Norway rat)	Nek1	NP_001099552.2
Gallus gallus (chicken)	NEK1	XP_420401.3
Homo sapiens (human)	NEK1	NP_001186326.1
	NEK1	XP_001083275.1
Canis lupus familiaris (dog)	NEK1	XP_543184.2
Mus musculus (house mouse)	Nek1	NP_780298.2
Pan troglodytes (chimpanzee)	NEK1	XP_526727.2
Danio rerio (zebrafish)	nek1	XP_005160601.1
Xenopus tropicalis (tropical clawed frog)	nek1	NP_001090680.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

NEK1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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