NEFH cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NEFH
Entrez Gene ID	4744
Full Name	neurofilament heavy
Synonyms	CMT2CC,NFH
General protein information	Preferred Names neurofilament heavy Names neurofilament heavy polypeptide 200 kDa neurofilament protein NF-H neurofilament triplet H protein neurofilament, heavy polypeptide 200kDa
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 22 Map Location: 22q12.2
Summary	Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene. [provided by RefSeq, Oct 2008].
Disorder MIM:	162230
Disorder Html:	?{Amyotrophic lateral sclerosis, susceptibility to}, 105400 (3); Charcot-Marie-Tooth disease, axonal, type 2CC, 616924 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011530200.2	XP_011528502.1	neurofilament heavy polypeptide isoform X1
NM_021076.4	NP_066554.2	neurofilament heavy polypeptide
NM_021076.3	NP_066554.2	neurofilament heavy polypeptide

Pathways from BioSystems

Homologies

Homo sapiens (human)	NEFH	NP_066554.2
Mus musculus (house mouse)	Nefh	NP_035034.2
Rattus norvegicus (Norway rat)	Nefh	NP_036739.2
Pan troglodytes (chimpanzee)	NEFH	XP_515058.3
Canis lupus familiaris (dog)	NEFH	NP_001003352.1
Bos taurus (cattle)	NEFH	XP_005218170.1
Gallus gallus (chicken)	NEFH	XP_415310.4

Gene Ontology
Bibliography

Related articles in PubMed

Comparison of elevated phosphorylated neurofilament heavy chains in serum and cerebrospinal fluid of patients with amyotrophic lateral sclerosis.
De Schaepdryver M, Jeromin A, Gille B, Claeys KG, Herbst V, Brix B, Van Damme P, Poesen K
Journal of neurology, neurosurgery, and psychiatry(2017 Oct)

Cryptic amyloidogenic elements in mutant NEFH causing Charcot-Marie-Tooth 2 trigger aggresome formation and neuronal death.
Jacquier A, Delorme C, Belotti E, Juntas-Morales R, Sol? G, Dubourg O, Giroux M, Maurage CA, Castellani V, Rebelo A, Abrams A, Z?chner S, Stojkovic T, Schaeffer L, Latour P
Acta neuropathologica communications5(1)55(2017 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

data support the use of Cebrospinal fluid phosphorylated NFH as a prognostic biomarker for amyotrophic lateral sclerosis.
Title: Phosphorylated neurofilament heavy chain: A biomarker of survival for C9ORF72-associated amyotrophic lateral sclerosis.

Unique deletions of two nucleotides causing frameshifts near the end of the NEFH coding sequence were identified in two Charcot-Marie-Tooth disease families. Using electroporation of chick embryo spinal cord, confirmed that NEFH mutants form aggregates in vivo and trigger apoptosis of spinal cord neurons.
Title: Cryptic amyloidogenic elements in mutant NEFH causing Charcot-Marie-Tooth 2 trigger aggresome formation and neuronal death.

Results provide evidence that in particular pNfH can be used as a good diagnostic biomarker of ALS at the diagnostic stage. Moreover, results indicate that NfL may be useful in monitoring disease progression in a subset of patients.
Title: Neurofilament markers for ALS correlate with extent of upper and lower motor neuron disease.

Study found a significant increase of pNF-H levels in both plasma and CSF in amyotrophic lateral sclerosis patients
Title: Phosphorylated neurofilament heavy chain levels in paired plasma and CSF of amyotrophic lateral sclerosis.

Level of neurofilament heavy chain and light chains were significantly elevated in the cerebrospinal fluid of Amyotrophic Lateral Sclerosis (ALS) patients compared to healthy controls/controls without parenchymal central nervous system involvement and ALS mimic disease patients.
Title: Neurofilaments as Biomarkers for Amyotrophic Lateral Sclerosis: A Systematic Review and Meta-Analysis.

The following NEFH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEFH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18204	NM_021076.4 Latest version!	Homo sapiens neurofilament heavy (NEFH), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OHu59241	XM_011530200.2 Latest version!	Homo sapiens neurofilament heavy (NEFH), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
View Old Accession Versions >>
OHu18204	NM_021076.3	Homo sapiens neurofilament heavy (NEFH), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NEFH ( NM_021076.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_066554.2 Homo sapiens neurofilament heavy (NEFH), mRNA.

Price & Availability↑

CloneID	OHu18204
Clone ID Related Accession (Same CDS sequence)	NM_021076.4 , NM_021076.3
Accession Version	NM_021076.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3063bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	neurofilament heavy polypeptide
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC000035.2 and AF203032.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_021076.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF203032.1, BC073969.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000310624.7/ ENSP00000311997.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END##

ATGATGAGCT TCGGCGGCGC GGACGCGCTG CTGGGCGCCC CGTTCGCGCC GCTGCATGGC 
GGCGGCAGCC TCCACTACGC GCTAGCCCGA AAGGGTGGCG CAGGCGGGAC GCGCTCCGCC 
GCTGGCTCCT CCAGCGGCTT CCACTCGTGG ACACGGACGT CCGTGAGCTC CGTGTCCGCC 
TCGCCCAGCC GCTTCCGTGG CGCAGGCGCC GCCTCAAGCA CCGACTCGCT GGACACGCTG 
AGCAACGGGC CGGAGGGCTG CATGGTGGCG GTGGCCACCT CACGCAGTGA GAAGGAGCAG 
CTGCAGGCGC TGAACGACCG CTTCGCCGGG TACATCGACA AGGTGCGGCA GCTGGAGGCG 
CACAACCGCA GCCTGGAGGG CGAGGCTGCG GCGCTGCGGC AGCAGCAGGC GGGCCGCTCC 
GCTATGGGCG AGCTGTACGA GCGCGAGGTC CGCGAGATGC GCGGCGCGGT GCTGCGCCTG 
GGCGCGGCGC GCGGTCAGCT ACGCCTGGAG CAGGAGCACC TGCTCGAGGA CATCGCGCAC 
GTGCGCCAGC GCCTAGACGA CGAGGCCCGG CAGCGAGAGG AGGCCGAGGC GGCGGCCCGC 
GCGCTGGCGC GCTTCGCGCA GGAGGCCGAG GCGGCGCGCG TGGACCTGCA GAAGAAGGCG 
CAGGCGCTGC AGGAGGAGTG CGGCTACCTG CGGCGCCACC ACCAGGAAGA GGTGGGCGAG 
CTGCTCGGCC AGATCCAGGG CTCCGGCGCC GCGCAGGCGC AGATGCAGGC CGAGACGCGC 
GACGCCCTGA AGTGCGACGT GACGTCGGCG CTGCGCGAGA TTCGCGCGCA GCTTGAAGGC 
CACGCGGTGC AGAGCACGCT GCAGTCCGAG GAGTGGTTCC GAGTGAGGCT GGACCGACTG 
TCGGAGGCAG CCAAGGTGAA CACAGACGCT ATGCGCTCAG CGCAGGAGGA GATAACTGAG 
TACCGGCGTC AGCTGCAGGC CAGGACCACA GAGCTGGAGG CACTGAAAAG CACCAAGGAC 
TCACTGGAGA GGCAGCGCTC TGAGCTGGAG GACCGTCATC AGGCCGACAT TGCCTCCTAC 
CAGGAAGCCA TTCAGCAGCT GGACGCTGAG CTGAGGAACA CCAAGTGGGA GATGGCCGCC 
CAGCTGCGAG AATACCAGGA CCTGCTCAAT GTCAAGATGG CTCTGGATAT AGAGATAGCC 
GCTTACAGAA AACTCCTGGA AGGTGAAGAG TGTCGGATTG GCTTTGGCCC AATTCCTTTC 
TCGCTTCCAG AAGGACTCCC CAAAATTCCC TCTGTGTCCA CTCACATAAA GGTGAAAAGC 
GAAGAGAAGA TCAAAGTGGT GGAGAAGTCT GAGAAAGAAA CTGTGATTGT GGAGGAACAG 
ACAGAGGAGA CCCAAGTGAC TGAAGAAGTG ACTGAAGAAG AGGAGAAAGA GGCCAAAGAG 
GAGGAGGGCA AGGAGGAAGA AGGGGGTGAA GAAGAGGAGG CAGAAGGGGG AGAAGAAGAA 
ACAAAGTCTC CCCCAGCAGA AGAGGCTGCA TCCCCAGAGA AGGAAGCCAA GTCACCAGTA 
AAGGAAGAGG CAAAGTCACC GGCTGAGGCC AAGTCCCCAG AGAAGGAGGA AGCAAAATCC 
CCAGCCGAAG TCAAGTCCCC TGAGAAGGCC AAGTCTCCAG CAAAGGAAGA GGCAAAGTCA 
CCGCCTGAGG CCAAGTCCCC AGAGAAGGAG GAAGCAAAAT CTCCAGCTGA GGTCAAGTCC 
CCCGAGAAGG CCAAGTCCCC AGCAAAGGAA GAGGCAAAGT CACCGGCTGA GGCCAAGTCT 
CCAGAGAAGG CCAAGTCCCC AGTGAAGGAA GAAGCAAAGT CACCGGCTGA GGCCAAGTCC 
CCAGTGAAGG AAGAAGCAAA ATCTCCAGCT GAGGTCAAGT CCCCGGAAAA GGCCAAGTCT 
CCAACGAAGG AGGAAGCAAA GTCCCCTGAG AAGGCCAAGT CCCCAGAGAA GGAAGAGGCC 
AAGTCCCCTG AGAAGGCCAA GTCCCCAGTG AAGGCAGAAG CAAAGTCCCC TGAGAAGGCC 
AAGTCCCCAG TGAAGGCAGA AGCAAAGTCC CCTGAGAAGG CCAAGTCCCC AGTGAAGGAA 
GAAGCAAAGT CCCCTGAGAA GGCCAAGTCC CCAGTGAAGG AAGAAGCAAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAAGAAGCA AAGACCCCCG AGAAGGCCAA GTCCCCAGTG 
AAGGAAGAAG CTAAGTCCCC AGAGAAGGCC AAGTCCCCAG AGAAGGCCAA GACTCTTGAT 
GTGAAGTCTC CAGAAGCCAA GACTCCAGCG AAGGAGGAAG CAAGGTCCCC TGCAGACAAA 
TTCCCTGAAA AGGCCAAAAG CCCTGTCAAG GAGGAGGTCA AGTCCCCAGA GAAGGCGAAA 
TCTCCCCTGA AGGAGGATGC CAAGGCCCCT GAGAAGGAGA TCCCAAAAAA GGAAGAGGTG 
AAGTCCCCAG TGAAGGAGGA GGAGAAGCCC CAGGAGGTGA AAGTCAAAGA GCCCCCAAAG 
AAGGCAGAGG AAGAGAAAGC CCCTGCCACA CCAAAAACAG AGGAGAAGAA GGACAGCAAG 
AAAGAGGAGG CACCCAAGAA GGAGGCTCCA AAGCCCAAGG TGGAGGAGAA GAAGGAACCT 
GCTGTCGAAA AGCCCAAAGA ATCCAAAGTT GAAGCCAAGA AGGAAGAGGC TGAAGATAAG 
AAAAAAGTCC CCACCCCAGA GAAGGAGGCT CCTGCCAAGG TGGAGGTGAA GGAAGACGCT 
AAACCCAAAG AAAAGACAGA GGTAGCCAAG AAGGAACCAG ATGATGCCAA GGCCAAGGAA 
CCCAGCAAAC CAGCAGAGAA GAAGGAGGCA GCACCGGAGA AAAAAGACAC CAAGGAGGAG 
AAGGCCAAGA AGCCTGAGGA GAAACCCAAG ACAGAGGCCA AAGCCAAGGA AGATGACAAG 
ACCCTCTCAA AAGAGCCTAG CAAGCCTAAG GCAGAAAAGG CTGAAAAATC CTCCAGCACA 
GACCAAAAAG ACAGCAAGCC TCCAGAGAAG GCCACAGAAG ACAAGGCCGC CAAGGGGAAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066554.2
CDS	46..3108
Translation	MMSFGGADALLGAPFAPLHGGGSLHYALARKGGAGGTRSAAGSSSGFHSWTRTSVSSVSASPSRFRGAGAASSTDSLDTLSNGPEGCMVAVATSRSEKEQLQALNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRSAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAAARALARFAQEAEAARVDLQKKAQALQEECGYLRRHHQEEVGELLGQIQGSGAAQAQMQAETRDALKCDVTSALREIRAQLEGHAVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEITEYRRQLQARTTELEALKSTKDSLERQRSELEDRHQADIASYQEAIQQLDAELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPIPFSLPEGLPKIPSVSTHIKVKSEEKIKVVEKSEKETVIVEEQTEETQVTEEVTEEEEKEAKEEEGKEEEGGEEEEAEGGEEETKSPPAEEAASPEKEAKSPVKEEAKSPAEAKSPEKEEAKSPAEVKSPEKAKSPAKEEAKSPPEAKSPEKEEAKSPAEVKSPEKAKSPAKEEAKSPAEAKSPEKAKSPVKEEAKSPAEAKSPVKEEAKSPAEVKSPEKAKSPTKEEAKSPEKAKSPEKEEAKSPEKAKSPVKAEAKSPEKAKSPVKAEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKTPEKAKSPVKEEAKSPEKAKSPEKAKTLDVKSPEAKTPAKEEARSPADKFPEKAKSPVKEEVKSPEKAKSPLKEDAKAPEKEIPKKEEVKSPVKEEEKPQEVKVKEPPKKAEEEKAPATPKTEEKKDSKKEEAPKKEAPKPKVEEKKEPAVEKPKESKVEAKKEEAEDKKKVPTPEKEAPAKVEVKEDAKPKEKTEVAKKEPDDAKAKEPSKPAEKKEAAPEKKDTKEEKAKKPEEKPKTEAKAKEDDKTLSKEPSKPKAEKAEKSSSTDQKDSKPPEKATEDKAAKGK

Target ORF information:

RefSeq Version	NM_021076.4
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofilament heavy (NEFH), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021076.4

ORF Insert Sequence:

ATGATGAGCT TCGGCGGCGC GGACGCGCTG CTGGGCGCCC CGTTCGCGCC GCTGCATGGC 
GGCGGCAGCC TCCACTACGC GCTAGCCCGA AAGGGTGGCG CAGGCGGGAC GCGCTCCGCC 
GCTGGCTCCT CCAGCGGCTT CCACTCGTGG ACACGGACGT CCGTGAGCTC CGTGTCCGCC 
TCGCCCAGCC GCTTCCGTGG CGCAGGCGCC GCCTCAAGCA CCGACTCGCT GGACACGCTG 
AGCAACGGGC CGGAGGGCTG CATGGTGGCG GTGGCCACCT CACGCAGTGA GAAGGAGCAG 
CTGCAGGCGC TGAACGACCG CTTCGCCGGG TACATCGACA AGGTGCGGCA GCTGGAGGCG 
CACAACCGCA GCCTGGAGGG CGAGGCTGCG GCGCTGCGGC AGCAGCAGGC GGGCCGCTCC 
GCTATGGGCG AGCTGTACGA GCGCGAGGTC CGCGAGATGC GCGGCGCGGT GCTGCGCCTG 
GGCGCGGCGC GCGGTCAGCT ACGCCTGGAG CAGGAGCACC TGCTCGAGGA CATCGCGCAC 
GTGCGCCAGC GCCTAGACGA CGAGGCCCGG CAGCGAGAGG AGGCCGAGGC GGCGGCCCGC 
GCGCTGGCGC GCTTCGCGCA GGAGGCCGAG GCGGCGCGCG TGGACCTGCA GAAGAAGGCG 
CAGGCGCTGC AGGAGGAGTG CGGCTACCTG CGGCGCCACC ACCAGGAAGA GGTGGGCGAG 
CTGCTCGGCC AGATCCAGGG CTCCGGCGCC GCGCAGGCGC AGATGCAGGC CGAGACGCGC 
GACGCCCTGA AGTGCGACGT GACGTCGGCG CTGCGCGAGA TTCGCGCGCA GCTTGAAGGC 
CACGCGGTGC AGAGCACGCT GCAGTCCGAG GAGTGGTTCC GAGTGAGGCT GGACCGACTG 
TCGGAGGCAG CCAAGGTGAA CACAGACGCT ATGCGCTCAG CGCAGGAGGA GATAACTGAG 
TACCGGCGTC AGCTGCAGGC CAGGACCACA GAGCTGGAGG CACTGAAAAG CACCAAGGAC 
TCACTGGAGA GGCAGCGCTC TGAGCTGGAG GACCGTCATC AGGCCGACAT TGCCTCCTAC 
CAGGAAGCCA TTCAGCAGCT GGACGCTGAG CTGAGGAACA CCAAGTGGGA GATGGCCGCC 
CAGCTGCGAG AATACCAGGA CCTGCTCAAT GTCAAGATGG CTCTGGATAT AGAGATAGCC 
GCTTACAGAA AACTCCTGGA AGGTGAAGAG TGTCGGATTG GCTTTGGCCC AATTCCTTTC 
TCGCTTCCAG AAGGACTCCC CAAAATTCCC TCTGTGTCCA CTCACATAAA GGTGAAAAGC 
GAAGAGAAGA TCAAAGTGGT GGAGAAGTCT GAGAAAGAAA CTGTGATTGT GGAGGAACAG 
ACAGAGGAGA CCCAAGTGAC TGAAGAAGTG ACTGAAGAAG AGGAGAAAGA GGCCAAAGAG 
GAGGAGGGCA AGGAGGAAGA AGGGGGTGAA GAAGAGGAGG CAGAAGGGGG AGAAGAAGAA 
ACAAAGTCTC CCCCAGCAGA AGAGGCTGCA TCCCCAGAGA AGGAAGCCAA GTCACCAGTA 
AAGGAAGAGG CAAAGTCACC GGCTGAGGCC AAGTCCCCAG AGAAGGAGGA AGCAAAATCC 
CCAGCCGAAG TCAAGTCCCC TGAGAAGGCC AAGTCTCCAG CAAAGGAAGA GGCAAAGTCA 
CCGCCTGAGG CCAAGTCCCC AGAGAAGGAG GAAGCAAAAT CTCCAGCTGA GGTCAAGTCC 
CCCGAGAAGG CCAAGTCCCC AGCAAAGGAA GAGGCAAAGT CACCGGCTGA GGCCAAGTCT 
CCAGAGAAGG CCAAGTCCCC AGTGAAGGAA GAAGCAAAGT CACCGGCTGA GGCCAAGTCC 
CCAGTGAAGG AAGAAGCAAA ATCTCCAGCT GAGGTCAAGT CCCCGGAAAA GGCCAAGTCT 
CCAACGAAGG AGGAAGCAAA GTCCCCTGAG AAGGCCAAGT CCCCAGAGAA GGAAGAGGCC 
AAGTCCCCTG AGAAGGCCAA GTCCCCAGTG AAGGCAGAAG CAAAGTCCCC TGAGAAGGCC 
AAGTCCCCAG TGAAGGCAGA AGCAAAGTCC CCTGAGAAGG CCAAGTCCCC AGTGAAGGAA 
GAAGCAAAGT CCCCTGAGAA GGCCAAGTCC CCAGTGAAGG AAGAAGCAAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAAGAAGCA AAGACCCCCG AGAAGGCCAA GTCCCCAGTG 
AAGGAAGAAG CTAAGTCCCC AGAGAAGGCC AAGTCCCCAG AGAAGGCCAA GACTCTTGAT 
GTGAAGTCTC CAGAAGCCAA GACTCCAGCG AAGGAGGAAG CAAGGTCCCC TGCAGACAAA 
TTCCCTGAAA AGGCCAAAAG CCCTGTCAAG GAGGAGGTCA AGTCCCCAGA GAAGGCGAAA 
TCTCCCCTGA AGGAGGATGC CAAGGCCCCT GAGAAGGAGA TCCCAAAAAA GGAAGAGGTG 
AAGTCCCCAG TGAAGGAGGA GGAGAAGCCC CAGGAGGTGA AAGTCAAAGA GCCCCCAAAG 
AAGGCAGAGG AAGAGAAAGC CCCTGCCACA CCAAAAACAG AGGAGAAGAA GGACAGCAAG 
AAAGAGGAGG CACCCAAGAA GGAGGCTCCA AAGCCCAAGG TGGAGGAGAA GAAGGAACCT 
GCTGTCGAAA AGCCCAAAGA ATCCAAAGTT GAAGCCAAGA AGGAAGAGGC TGAAGATAAG 
AAAAAAGTCC CCACCCCAGA GAAGGAGGCT CCTGCCAAGG TGGAGGTGAA GGAAGACGCT 
AAACCCAAAG AAAAGACAGA GGTAGCCAAG AAGGAACCAG ATGATGCCAA GGCCAAGGAA 
CCCAGCAAAC CAGCAGAGAA GAAGGAGGCA GCACCGGAGA AAAAAGACAC CAAGGAGGAG 
AAGGCCAAGA AGCCTGAGGA GAAACCCAAG ACAGAGGCCA AAGCCAAGGA AGATGACAAG 
ACCCTCTCAA AAGAGCCTAG CAAGCCTAAG GCAGAAAAGG CTGAAAAATC CTCCAGCACA 
GACCAAAAAG ACAGCAAGCC TCCAGAGAAG GCCACAGAAG ACAAGGCCGC CAAGGGGAAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEFH ( NM_021076.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_066554.2 Homo sapiens neurofilament heavy (NEFH), mRNA.

Price & Availability↑

CloneID	OHu18204
Clone ID Related Accession (Same CDS sequence)	NM_021076.4 , NM_021076.3
Accession Version	NM_021076.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3063bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-17
Organism	Homo sapiens(human)
Product	neurofilament heavy polypeptide
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC000035.2 and AF203032.1. This sequence is a reference standard in the RefSeqGene project. On Aug 15, 2008 this sequence version replaced NM_021076.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302660.1, AF203032.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

ATGATGAGCT TCGGCGGCGC GGACGCGCTG CTGGGCGCCC CGTTCGCGCC GCTGCATGGC 
GGCGGCAGCC TCCACTACGC GCTAGCCCGA AAGGGTGGCG CAGGCGGGAC GCGCTCCGCC 
GCTGGCTCCT CCAGCGGCTT CCACTCGTGG ACACGGACGT CCGTGAGCTC CGTGTCCGCC 
TCGCCCAGCC GCTTCCGTGG CGCAGGCGCC GCCTCAAGCA CCGACTCGCT GGACACGCTG 
AGCAACGGGC CGGAGGGCTG CATGGTGGCG GTGGCCACCT CACGCAGTGA GAAGGAGCAG 
CTGCAGGCGC TGAACGACCG CTTCGCCGGG TACATCGACA AGGTGCGGCA GCTGGAGGCG 
CACAACCGCA GCCTGGAGGG CGAGGCTGCG GCGCTGCGGC AGCAGCAGGC GGGCCGCTCC 
GCTATGGGCG AGCTGTACGA GCGCGAGGTC CGCGAGATGC GCGGCGCGGT GCTGCGCCTG 
GGCGCGGCGC GCGGTCAGCT ACGCCTGGAG CAGGAGCACC TGCTCGAGGA CATCGCGCAC 
GTGCGCCAGC GCCTAGACGA CGAGGCCCGG CAGCGAGAGG AGGCCGAGGC GGCGGCCCGC 
GCGCTGGCGC GCTTCGCGCA GGAGGCCGAG GCGGCGCGCG TGGACCTGCA GAAGAAGGCG 
CAGGCGCTGC AGGAGGAGTG CGGCTACCTG CGGCGCCACC ACCAGGAAGA GGTGGGCGAG 
CTGCTCGGCC AGATCCAGGG CTCCGGCGCC GCGCAGGCGC AGATGCAGGC CGAGACGCGC 
GACGCCCTGA AGTGCGACGT GACGTCGGCG CTGCGCGAGA TTCGCGCGCA GCTTGAAGGC 
CACGCGGTGC AGAGCACGCT GCAGTCCGAG GAGTGGTTCC GAGTGAGGCT GGACCGACTG 
TCGGAGGCAG CCAAGGTGAA CACAGACGCT ATGCGCTCAG CGCAGGAGGA GATAACTGAG 
TACCGGCGTC AGCTGCAGGC CAGGACCACA GAGCTGGAGG CACTGAAAAG CACCAAGGAC 
TCACTGGAGA GGCAGCGCTC TGAGCTGGAG GACCGTCATC AGGCCGACAT TGCCTCCTAC 
CAGGAAGCCA TTCAGCAGCT GGACGCTGAG CTGAGGAACA CCAAGTGGGA GATGGCCGCC 
CAGCTGCGAG AATACCAGGA CCTGCTCAAT GTCAAGATGG CTCTGGATAT AGAGATAGCC 
GCTTACAGAA AACTCCTGGA AGGTGAAGAG TGTCGGATTG GCTTTGGCCC AATTCCTTTC 
TCGCTTCCAG AAGGACTCCC CAAAATTCCC TCTGTGTCCA CTCACATAAA GGTGAAAAGC 
GAAGAGAAGA TCAAAGTGGT GGAGAAGTCT GAGAAAGAAA CTGTGATTGT GGAGGAACAG 
ACAGAGGAGA CCCAAGTGAC TGAAGAAGTG ACTGAAGAAG AGGAGAAAGA GGCCAAAGAG 
GAGGAGGGCA AGGAGGAAGA AGGGGGTGAA GAAGAGGAGG CAGAAGGGGG AGAAGAAGAA 
ACAAAGTCTC CCCCAGCAGA AGAGGCTGCA TCCCCAGAGA AGGAAGCCAA GTCACCAGTA 
AAGGAAGAGG CAAAGTCACC GGCTGAGGCC AAGTCCCCAG AGAAGGAGGA AGCAAAATCC 
CCAGCCGAAG TCAAGTCCCC TGAGAAGGCC AAGTCTCCAG CAAAGGAAGA GGCAAAGTCA 
CCGCCTGAGG CCAAGTCCCC AGAGAAGGAG GAAGCAAAAT CTCCAGCTGA GGTCAAGTCC 
CCCGAGAAGG CCAAGTCCCC AGCAAAGGAA GAGGCAAAGT CACCGGCTGA GGCCAAGTCT 
CCAGAGAAGG CCAAGTCCCC AGTGAAGGAA GAAGCAAAGT CACCGGCTGA GGCCAAGTCC 
CCAGTGAAGG AAGAAGCAAA ATCTCCAGCT GAGGTCAAGT CCCCGGAAAA GGCCAAGTCT 
CCAACGAAGG AGGAAGCAAA GTCCCCTGAG AAGGCCAAGT CCCCAGAGAA GGAAGAGGCC 
AAGTCCCCTG AGAAGGCCAA GTCCCCAGTG AAGGCAGAAG CAAAGTCCCC TGAGAAGGCC 
AAGTCCCCAG TGAAGGCAGA AGCAAAGTCC CCTGAGAAGG CCAAGTCCCC AGTGAAGGAA 
GAAGCAAAGT CCCCTGAGAA GGCCAAGTCC CCAGTGAAGG AAGAAGCAAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAAGAAGCA AAGACCCCCG AGAAGGCCAA GTCCCCAGTG 
AAGGAAGAAG CTAAGTCCCC AGAGAAGGCC AAGTCCCCAG AGAAGGCCAA GACTCTTGAT 
GTGAAGTCTC CAGAAGCCAA GACTCCAGCG AAGGAGGAAG CAAGGTCCCC TGCAGACAAA 
TTCCCTGAAA AGGCCAAAAG CCCTGTCAAG GAGGAGGTCA AGTCCCCAGA GAAGGCGAAA 
TCTCCCCTGA AGGAGGATGC CAAGGCCCCT GAGAAGGAGA TCCCAAAAAA GGAAGAGGTG 
AAGTCCCCAG TGAAGGAGGA GGAGAAGCCC CAGGAGGTGA AAGTCAAAGA GCCCCCAAAG 
AAGGCAGAGG AAGAGAAAGC CCCTGCCACA CCAAAAACAG AGGAGAAGAA GGACAGCAAG 
AAAGAGGAGG CACCCAAGAA GGAGGCTCCA AAGCCCAAGG TGGAGGAGAA GAAGGAACCT 
GCTGTCGAAA AGCCCAAAGA ATCCAAAGTT GAAGCCAAGA AGGAAGAGGC TGAAGATAAG 
AAAAAAGTCC CCACCCCAGA GAAGGAGGCT CCTGCCAAGG TGGAGGTGAA GGAAGACGCT 
AAACCCAAAG AAAAGACAGA GGTAGCCAAG AAGGAACCAG ATGATGCCAA GGCCAAGGAA 
CCCAGCAAAC CAGCAGAGAA GAAGGAGGCA GCACCGGAGA AAAAAGACAC CAAGGAGGAG 
AAGGCCAAGA AGCCTGAGGA GAAACCCAAG ACAGAGGCCA AAGCCAAGGA AGATGACAAG 
ACCCTCTCAA AAGAGCCTAG CAAGCCTAAG GCAGAAAAGG CTGAAAAATC CTCCAGCACA 
GACCAAAAAG ACAGCAAGCC TCCAGAGAAG GCCACAGAAG ACAAGGCCGC CAAGGGGAAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066554.2
CDS	72..3134
Translation	MMSFGGADALLGAPFAPLHGGGSLHYALARKGGAGGTRSAAGSSSGFHSWTRTSVSSVSASPSRFRGAGAASSTDSLDTLSNGPEGCMVAVATSRSEKEQLQALNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRSAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAAARALARFAQEAEAARVDLQKKAQALQEECGYLRRHHQEEVGELLGQIQGSGAAQAQMQAETRDALKCDVTSALREIRAQLEGHAVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEITEYRRQLQARTTELEALKSTKDSLERQRSELEDRHQADIASYQEAIQQLDAELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPIPFSLPEGLPKIPSVSTHIKVKSEEKIKVVEKSEKETVIVEEQTEETQVTEEVTEEEEKEAKEEEGKEEEGGEEEEAEGGEEETKSPPAEEAASPEKEAKSPVKEEAKSPAEAKSPEKEEAKSPAEVKSPEKAKSPAKEEAKSPPEAKSPEKEEAKSPAEVKSPEKAKSPAKEEAKSPAEAKSPEKAKSPVKEEAKSPAEAKSPVKEEAKSPAEVKSPEKAKSPTKEEAKSPEKAKSPEKEEAKSPEKAKSPVKAEAKSPEKAKSPVKAEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKTPEKAKSPVKEEAKSPEKAKSPEKAKTLDVKSPEAKTPAKEEARSPADKFPEKAKSPVKEEVKSPEKAKSPLKEDAKAPEKEIPKKEEVKSPVKEEEKPQEVKVKEPPKKAEEEKAPATPKTEEKKDSKKEEAPKKEAPKPKVEEKKEPAVEKPKESKVEAKKEEAEDKKKVPTPEKEAPAKVEVKEDAKPKEKTEVAKKEPDDAKAKEPSKPAEKKEAAPEKKDTKEEKAKKPEEKPKTEAKAKEDDKTLSKEPSKPKAEKAEKSSSTDQKDSKPPEKATEDKAAKGK

Target ORF information:

RefSeq Version	NM_021076.3
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofilament heavy (NEFH), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021076.3

ORF Insert Sequence:

ATGATGAGCT TCGGCGGCGC GGACGCGCTG CTGGGCGCCC CGTTCGCGCC GCTGCATGGC 
GGCGGCAGCC TCCACTACGC GCTAGCCCGA AAGGGTGGCG CAGGCGGGAC GCGCTCCGCC 
GCTGGCTCCT CCAGCGGCTT CCACTCGTGG ACACGGACGT CCGTGAGCTC CGTGTCCGCC 
TCGCCCAGCC GCTTCCGTGG CGCAGGCGCC GCCTCAAGCA CCGACTCGCT GGACACGCTG 
AGCAACGGGC CGGAGGGCTG CATGGTGGCG GTGGCCACCT CACGCAGTGA GAAGGAGCAG 
CTGCAGGCGC TGAACGACCG CTTCGCCGGG TACATCGACA AGGTGCGGCA GCTGGAGGCG 
CACAACCGCA GCCTGGAGGG CGAGGCTGCG GCGCTGCGGC AGCAGCAGGC GGGCCGCTCC 
GCTATGGGCG AGCTGTACGA GCGCGAGGTC CGCGAGATGC GCGGCGCGGT GCTGCGCCTG 
GGCGCGGCGC GCGGTCAGCT ACGCCTGGAG CAGGAGCACC TGCTCGAGGA CATCGCGCAC 
GTGCGCCAGC GCCTAGACGA CGAGGCCCGG CAGCGAGAGG AGGCCGAGGC GGCGGCCCGC 
GCGCTGGCGC GCTTCGCGCA GGAGGCCGAG GCGGCGCGCG TGGACCTGCA GAAGAAGGCG 
CAGGCGCTGC AGGAGGAGTG CGGCTACCTG CGGCGCCACC ACCAGGAAGA GGTGGGCGAG 
CTGCTCGGCC AGATCCAGGG CTCCGGCGCC GCGCAGGCGC AGATGCAGGC CGAGACGCGC 
GACGCCCTGA AGTGCGACGT GACGTCGGCG CTGCGCGAGA TTCGCGCGCA GCTTGAAGGC 
CACGCGGTGC AGAGCACGCT GCAGTCCGAG GAGTGGTTCC GAGTGAGGCT GGACCGACTG 
TCGGAGGCAG CCAAGGTGAA CACAGACGCT ATGCGCTCAG CGCAGGAGGA GATAACTGAG 
TACCGGCGTC AGCTGCAGGC CAGGACCACA GAGCTGGAGG CACTGAAAAG CACCAAGGAC 
TCACTGGAGA GGCAGCGCTC TGAGCTGGAG GACCGTCATC AGGCCGACAT TGCCTCCTAC 
CAGGAAGCCA TTCAGCAGCT GGACGCTGAG CTGAGGAACA CCAAGTGGGA GATGGCCGCC 
CAGCTGCGAG AATACCAGGA CCTGCTCAAT GTCAAGATGG CTCTGGATAT AGAGATAGCC 
GCTTACAGAA AACTCCTGGA AGGTGAAGAG TGTCGGATTG GCTTTGGCCC AATTCCTTTC 
TCGCTTCCAG AAGGACTCCC CAAAATTCCC TCTGTGTCCA CTCACATAAA GGTGAAAAGC 
GAAGAGAAGA TCAAAGTGGT GGAGAAGTCT GAGAAAGAAA CTGTGATTGT GGAGGAACAG 
ACAGAGGAGA CCCAAGTGAC TGAAGAAGTG ACTGAAGAAG AGGAGAAAGA GGCCAAAGAG 
GAGGAGGGCA AGGAGGAAGA AGGGGGTGAA GAAGAGGAGG CAGAAGGGGG AGAAGAAGAA 
ACAAAGTCTC CCCCAGCAGA AGAGGCTGCA TCCCCAGAGA AGGAAGCCAA GTCACCAGTA 
AAGGAAGAGG CAAAGTCACC GGCTGAGGCC AAGTCCCCAG AGAAGGAGGA AGCAAAATCC 
CCAGCCGAAG TCAAGTCCCC TGAGAAGGCC AAGTCTCCAG CAAAGGAAGA GGCAAAGTCA 
CCGCCTGAGG CCAAGTCCCC AGAGAAGGAG GAAGCAAAAT CTCCAGCTGA GGTCAAGTCC 
CCCGAGAAGG CCAAGTCCCC AGCAAAGGAA GAGGCAAAGT CACCGGCTGA GGCCAAGTCT 
CCAGAGAAGG CCAAGTCCCC AGTGAAGGAA GAAGCAAAGT CACCGGCTGA GGCCAAGTCC 
CCAGTGAAGG AAGAAGCAAA ATCTCCAGCT GAGGTCAAGT CCCCGGAAAA GGCCAAGTCT 
CCAACGAAGG AGGAAGCAAA GTCCCCTGAG AAGGCCAAGT CCCCAGAGAA GGAAGAGGCC 
AAGTCCCCTG AGAAGGCCAA GTCCCCAGTG AAGGCAGAAG CAAAGTCCCC TGAGAAGGCC 
AAGTCCCCAG TGAAGGCAGA AGCAAAGTCC CCTGAGAAGG CCAAGTCCCC AGTGAAGGAA 
GAAGCAAAGT CCCCTGAGAA GGCCAAGTCC CCAGTGAAGG AAGAAGCAAA GTCCCCTGAG 
AAGGCCAAGT CCCCAGTGAA GGAAGAAGCA AAGACCCCCG AGAAGGCCAA GTCCCCAGTG 
AAGGAAGAAG CTAAGTCCCC AGAGAAGGCC AAGTCCCCAG AGAAGGCCAA GACTCTTGAT 
GTGAAGTCTC CAGAAGCCAA GACTCCAGCG AAGGAGGAAG CAAGGTCCCC TGCAGACAAA 
TTCCCTGAAA AGGCCAAAAG CCCTGTCAAG GAGGAGGTCA AGTCCCCAGA GAAGGCGAAA 
TCTCCCCTGA AGGAGGATGC CAAGGCCCCT GAGAAGGAGA TCCCAAAAAA GGAAGAGGTG 
AAGTCCCCAG TGAAGGAGGA GGAGAAGCCC CAGGAGGTGA AAGTCAAAGA GCCCCCAAAG 
AAGGCAGAGG AAGAGAAAGC CCCTGCCACA CCAAAAACAG AGGAGAAGAA GGACAGCAAG 
AAAGAGGAGG CACCCAAGAA GGAGGCTCCA AAGCCCAAGG TGGAGGAGAA GAAGGAACCT 
GCTGTCGAAA AGCCCAAAGA ATCCAAAGTT GAAGCCAAGA AGGAAGAGGC TGAAGATAAG 
AAAAAAGTCC CCACCCCAGA GAAGGAGGCT CCTGCCAAGG TGGAGGTGAA GGAAGACGCT 
AAACCCAAAG AAAAGACAGA GGTAGCCAAG AAGGAACCAG ATGATGCCAA GGCCAAGGAA 
CCCAGCAAAC CAGCAGAGAA GAAGGAGGCA GCACCGGAGA AAAAAGACAC CAAGGAGGAG 
AAGGCCAAGA AGCCTGAGGA GAAACCCAAG ACAGAGGCCA AAGCCAAGGA AGATGACAAG 
ACCCTCTCAA AAGAGCCTAG CAAGCCTAAG GCAGAAAAGG CTGAAAAATC CTCCAGCACA 
GACCAAAAAG ACAGCAAGCC TCCAGAGAAG GCCACAGAAG ACAAGGCCGC CAAGGGGAAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NEFH ( XM_011530200.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011528502.1 Homo sapiens neurofilament heavy (NEFH), transcript variant X1, mRNA.

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CloneID	OHu59241
Clone ID Related Accession (Same CDS sequence)	XM_011530200.2
Accession Version	XM_011530200.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neurofilament heavy polypeptide isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530200.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAGCT TCGGCGGCGC GGACGCGCTG CTGGGCGCCC CGTTCGCGCC GCTGCATGGC 
GGCGGCAGCC TCCACTACGC GCTAGCCCGA AAGGGTGGCG CAGGCGGGAC GCGCTCCGCC 
GCTGGCTCCT CCAGCGGCTT CCACTCGTGG ACACGGACGT CCGTGAGCTC CGTGTCCGCC 
TCGCCCAGCC GCTTCCGTGG CGCAGGCGCC GCCTCAAGCA CCGACTCGCT GGACACGCTG 
AGCAACGGGC CGGAGGGCTG CATGGTGGCG GTGGCCACCT CACGCAGTGA GAAGGAGCAG 
CTGCAGGCGC TGAACGACCG CTTCGCCGGG TACATCGACA AGGTGCGGCA GCTGGAGGCG 
CACAACCGCA GCCTGGAGGG CGAGGCTGCG GCGCTGCGGC AGCAGCAGGC GGGCCGCTCC 
GCTATGGGCG AGCTGTACGA GCGCGAGGTC CGCGAGATGC GCGGCGCGGT GCTGCGCCTG 
GGCGCGGCGC GCGGTCAGCT ACGCCTGGAG CAGGAGCACC TGCTCGAGGA CATCGCGCAC 
GTGCGCCAGC GCCTAGACGA CGAGGCCCGG CAGCGAGAGG AGGCCGAGGC GGCGGCCCGC 
GCGCTGGCGC GCTTCGCGCA GGAGGCCGAG GCGGCGCGCG TGGACCTGCA GAAGAAGGCG 
CAGGCGCTGC AGGAGGAGTG CGGCTACCTG CGGCGCCACC ACCAGGAAGA GGTGGGCGAG 
CTGCTCGGCC AGATCCAGGG CTCCGGCGCC GCGCAGGCGC AGATGCAGGC CGAGACGCGC 
GACGCCCTGA AGTGCGACGT GACGTCGGCG CTGCGCGAGA TTCGCGCGCA GCTTGAAGGC 
CACGCGGTGC AGAGCACGCT GCAGTCCGAG GAGTGGTTCC GAGTGAGGCT GGACCGACTG 
TCGGAGGCAG CCAAGGTGAA CACAGACGCT ATGCGCTCAG CGCAGGAGGA GATAACTGAG 
TACCGGCGTC AGCTGCAGGC CAGGACCACA GAGCTGGAGG CACTGAAAAG CACCAAGGAC 
TCACTGGAGA GGCAGCGCTC TGAGCTGGAG GACCGTCATC AGGCCGACAT TGCCTCCTAC 
CAGGAAGCCA TTCAGCAGCT GGACGCTGAG CTGAGGAACA CCAAGTGGGA GATGGCCGCC 
CAGCTGCGAG AATACCAGGA CCTGCTCAAT GTCAAGATGG CTCTGGATAT AGAGATAGCC 
GCTTACAGAA AACTCCTGGA AGGTGAAGAG TGTCGGATTG GCTTTGGCCC AATTCCTTTC 
TCGCTTCCAG AAGGACTCCC CAAAATTCCC TCTGTGTCCA CTCACATAAA GGTGAAAAGC 
GAAGAGAAGA TCAAAGTGGT GGAGAAGTCT GAGAAAGAAA CTGTGATTGT GGAGGAACAG 
ACAGAGGAGA CCCAAGTGAC TGAAGAAGTG ACTGAAGAAG AGGAGAAAGA GGCCAAAGAG 
GAGGAGGGCA AGGAGGAAGA AGGGGGTGAA GAAGAGGAGG CAGAAGGGGG AGAAGAAGAA 
ACAAAGTCTC CCCCAGCAGA AGAGGCTGCA TCCCCAGAGA AGGAAGCCAA GTCACCAGTA 
AAGGAAGAGG CAAAGTCACC GGCTGAGGCC AAGTCCCCAG AGAAGGAGGA AGCAAAATCC 
CCAGCCGAAG TCAAGTCCCC TGAGAAGGCC AAGTCCCCTG AGAAGGCCAA GTCCCCAGAG 
AAGGAAGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCAG TGAAGGCAGA AGCAAAGTCC 
CCTGAGAAGG CCAAGTCCCC AGTGAAGGCA GAAGCAAAGT CCCCTGAGAA GGCCAAGTCC 
CCAGTGAAGG AAGAAGCAAA GTCCCCTGAG AAGGCCAAGT CCCCAGTGAA GGAAGAAGCA 
AAGTCCCCTG AGAAGGCCAA GTCCCCAGTG AAGGAAGAAG CAAAGACCCC CGAGAAGGCC 
AAGTCCCCAG TGAAGGAAGA AGCTAAGTCC CCAGAGAAGG CCAAGTCCCC AGAGAAGGCC 
AAGACTCTTG ATGTGAAGTC TCCAGAAGCC AAGACTCCAG CGAAGGAGGA AGCAAGGTCC 
CCTGCAGACA AATTCCCTGA AAAGGCCAAA AGCCCTGTCA AGGAGGAGGT CAAGTCCCCA 
GAGAAGGCGA AATCTCCCCT GAAGGAGGAT GCCAAGGCCC CTGAGAAGGA GATCCCAAAA 
AAGGAAGAGG TGAAGTCCCC AGTGAAGGAG GAGGAGAAGC CCCAGGAGGT GAAAGTCAAA 
GAGCCCCCAA AGAAGGCAGA GGAAGAGAAA GCCCCTGCCA CACCAAAAAC AGAGGAGAAG 
AAGGACAGCA AGAAAGAGGA GGCACCCAAG AAGGAGGCTC CAAAGCCCAA GGTGGAGGAG 
AAGAAGGAAC CTGCTGTCGA AAAGCCCAAA GAATCCAAAG TTGAAGCCAA GAAGGAAGAG 
GCTGAAGATA AGAAAAAAGT CCCCACCCCA GAGAAGGAGG CTCCTGCCAA GGTGGAGGTG 
AAGGAAGACG CTAAACCCAA AGAAAAGACA GAGGTAGCCA AGAAGGAACC AGATGATGCC 
AAGGCCAAGG AACCCAGCAA ACCAGCAGAG AAGAAGGAGG CAGCACCGGA GAAAAAAGAC 
ACCAAGGAGG AGAAGGCCAA GAAGCCTGAG GAGAAACCCA AGACAGAGGC CAAAGCCAAG 
GAAGATGACA AGACCCTCTC AAAAGAGCCT AGCAAGCCTA AGGCAGAAAA GGCTGAAAAA 
TCCTCCAGCA CAGACCAAAA AGACAGCAAG CCTCCAGAGA AGGCCACAGA AGACAAGGCC 
GCCAAGGGGA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011528502.1
CDS	38..2812
Translation	MMSFGGADALLGAPFAPLHGGGSLHYALARKGGAGGTRSAAGSSSGFHSWTRTSVSSVSASPSRFRGAGAASSTDSLDTLSNGPEGCMVAVATSRSEKEQLQALNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRSAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAAARALARFAQEAEAARVDLQKKAQALQEECGYLRRHHQEEVGELLGQIQGSGAAQAQMQAETRDALKCDVTSALREIRAQLEGHAVQSTLQSEEWFRVRLDRLSEAAKVNTDAMRSAQEEITEYRRQLQARTTELEALKSTKDSLERQRSELEDRHQADIASYQEAIQQLDAELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPIPFSLPEGLPKIPSVSTHIKVKSEEKIKVVEKSEKETVIVEEQTEETQVTEEVTEEEEKEAKEEEGKEEEGGEEEEAEGGEEETKSPPAEEAASPEKEAKSPVKEEAKSPAEAKSPEKEEAKSPAEVKSPEKAKSPEKAKSPEKEEAKSPEKAKSPVKAEAKSPEKAKSPVKAEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKTPEKAKSPVKEEAKSPEKAKSPEKAKTLDVKSPEAKTPAKEEARSPADKFPEKAKSPVKEEVKSPEKAKSPLKEDAKAPEKEIPKKEEVKSPVKEEEKPQEVKVKEPPKKAEEEKAPATPKTEEKKDSKKEEAPKKEAPKPKVEEKKEPAVEKPKESKVEAKKEEAEDKKKVPTPEKEAPAKVEVKEDAKPKEKTEVAKKEPDDAKAKEPSKPAEKKEAAPEKKDTKEEKAKKPEEKPKTEAKAKEDDKTLSKEPSKPKAEKAEKSSSTDQKDSKPPEKATEDKAAKGK

Target ORF information:

RefSeq Version	XM_011530200.2
Organism	Homo sapiens(human)
Definition	Homo sapiens neurofilament heavy (NEFH), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011530200.2

ORF Insert Sequence:

ATGATGAGCT TCGGCGGCGC GGACGCGCTG CTGGGCGCCC CGTTCGCGCC GCTGCATGGC 
GGCGGCAGCC TCCACTACGC GCTAGCCCGA AAGGGTGGCG CAGGCGGGAC GCGCTCCGCC 
GCTGGCTCCT CCAGCGGCTT CCACTCGTGG ACACGGACGT CCGTGAGCTC CGTGTCCGCC 
TCGCCCAGCC GCTTCCGTGG CGCAGGCGCC GCCTCAAGCA CCGACTCGCT GGACACGCTG 
AGCAACGGGC CGGAGGGCTG CATGGTGGCG GTGGCCACCT CACGCAGTGA GAAGGAGCAG 
CTGCAGGCGC TGAACGACCG CTTCGCCGGG TACATCGACA AGGTGCGGCA GCTGGAGGCG 
CACAACCGCA GCCTGGAGGG CGAGGCTGCG GCGCTGCGGC AGCAGCAGGC GGGCCGCTCC 
GCTATGGGCG AGCTGTACGA GCGCGAGGTC CGCGAGATGC GCGGCGCGGT GCTGCGCCTG 
GGCGCGGCGC GCGGTCAGCT ACGCCTGGAG CAGGAGCACC TGCTCGAGGA CATCGCGCAC 
GTGCGCCAGC GCCTAGACGA CGAGGCCCGG CAGCGAGAGG AGGCCGAGGC GGCGGCCCGC 
GCGCTGGCGC GCTTCGCGCA GGAGGCCGAG GCGGCGCGCG TGGACCTGCA GAAGAAGGCG 
CAGGCGCTGC AGGAGGAGTG CGGCTACCTG CGGCGCCACC ACCAGGAAGA GGTGGGCGAG 
CTGCTCGGCC AGATCCAGGG CTCCGGCGCC GCGCAGGCGC AGATGCAGGC CGAGACGCGC 
GACGCCCTGA AGTGCGACGT GACGTCGGCG CTGCGCGAGA TTCGCGCGCA GCTTGAAGGC 
CACGCGGTGC AGAGCACGCT GCAGTCCGAG GAGTGGTTCC GAGTGAGGCT GGACCGACTG 
TCGGAGGCAG CCAAGGTGAA CACAGACGCT ATGCGCTCAG CGCAGGAGGA GATAACTGAG 
TACCGGCGTC AGCTGCAGGC CAGGACCACA GAGCTGGAGG CACTGAAAAG CACCAAGGAC 
TCACTGGAGA GGCAGCGCTC TGAGCTGGAG GACCGTCATC AGGCCGACAT TGCCTCCTAC 
CAGGAAGCCA TTCAGCAGCT GGACGCTGAG CTGAGGAACA CCAAGTGGGA GATGGCCGCC 
CAGCTGCGAG AATACCAGGA CCTGCTCAAT GTCAAGATGG CTCTGGATAT AGAGATAGCC 
GCTTACAGAA AACTCCTGGA AGGTGAAGAG TGTCGGATTG GCTTTGGCCC AATTCCTTTC 
TCGCTTCCAG AAGGACTCCC CAAAATTCCC TCTGTGTCCA CTCACATAAA GGTGAAAAGC 
GAAGAGAAGA TCAAAGTGGT GGAGAAGTCT GAGAAAGAAA CTGTGATTGT GGAGGAACAG 
ACAGAGGAGA CCCAAGTGAC TGAAGAAGTG ACTGAAGAAG AGGAGAAAGA GGCCAAAGAG 
GAGGAGGGCA AGGAGGAAGA AGGGGGTGAA GAAGAGGAGG CAGAAGGGGG AGAAGAAGAA 
ACAAAGTCTC CCCCAGCAGA AGAGGCTGCA TCCCCAGAGA AGGAAGCCAA GTCACCAGTA 
AAGGAAGAGG CAAAGTCACC GGCTGAGGCC AAGTCCCCAG AGAAGGAGGA AGCAAAATCC 
CCAGCCGAAG TCAAGTCCCC TGAGAAGGCC AAGTCCCCTG AGAAGGCCAA GTCCCCAGAG 
AAGGAAGAGG CCAAGTCCCC TGAGAAGGCC AAGTCCCCAG TGAAGGCAGA AGCAAAGTCC 
CCTGAGAAGG CCAAGTCCCC AGTGAAGGCA GAAGCAAAGT CCCCTGAGAA GGCCAAGTCC 
CCAGTGAAGG AAGAAGCAAA GTCCCCTGAG AAGGCCAAGT CCCCAGTGAA GGAAGAAGCA 
AAGTCCCCTG AGAAGGCCAA GTCCCCAGTG AAGGAAGAAG CAAAGACCCC CGAGAAGGCC 
AAGTCCCCAG TGAAGGAAGA AGCTAAGTCC CCAGAGAAGG CCAAGTCCCC AGAGAAGGCC 
AAGACTCTTG ATGTGAAGTC TCCAGAAGCC AAGACTCCAG CGAAGGAGGA AGCAAGGTCC 
CCTGCAGACA AATTCCCTGA AAAGGCCAAA AGCCCTGTCA AGGAGGAGGT CAAGTCCCCA 
GAGAAGGCGA AATCTCCCCT GAAGGAGGAT GCCAAGGCCC CTGAGAAGGA GATCCCAAAA 
AAGGAAGAGG TGAAGTCCCC AGTGAAGGAG GAGGAGAAGC CCCAGGAGGT GAAAGTCAAA 
GAGCCCCCAA AGAAGGCAGA GGAAGAGAAA GCCCCTGCCA CACCAAAAAC AGAGGAGAAG 
AAGGACAGCA AGAAAGAGGA GGCACCCAAG AAGGAGGCTC CAAAGCCCAA GGTGGAGGAG 
AAGAAGGAAC CTGCTGTCGA AAAGCCCAAA GAATCCAAAG TTGAAGCCAA GAAGGAAGAG 
GCTGAAGATA AGAAAAAAGT CCCCACCCCA GAGAAGGAGG CTCCTGCCAA GGTGGAGGTG 
AAGGAAGACG CTAAACCCAA AGAAAAGACA GAGGTAGCCA AGAAGGAACC AGATGATGCC 
AAGGCCAAGG AACCCAGCAA ACCAGCAGAG AAGAAGGAGG CAGCACCGGA GAAAAAAGAC 
ACCAAGGAGG AGAAGGCCAA GAAGCCTGAG GAGAAACCCA AGACAGAGGC CAAAGCCAAG 
GAAGATGACA AGACCCTCTC AAAAGAGCCT AGCAAGCCTA AGGCAGAAAA GGCTGAAAAA 
TCCTCCAGCA CAGACCAAAA AGACAGCAAG CCTCCAGAGA AGGCCACAGA AGACAAGGCC 
GCCAAGGGGA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Comparison of elevated phosphorylated neurofilament heavy chains in serum and cerebrospinal fluid of patients with amyotrophic lateral sclerosis.
Journal of neurology, neurosurgery, and psychiatry(2017 Oct)
De Schaepdryver M,Jeromin A,Gille B,Claeys KG,Herbst V,Brix B,Van Damme P,Poesen K

Cryptic amyloidogenic elements in mutant NEFH causing Charcot-Marie-Tooth 2 trigger aggresome formation and neuronal death.
Acta neuropathologica communications5(1)55(2017 Jul)
Jacquier A,Delorme C,Belotti E,Juntas-Morales R,Sol? G,Dubourg O,Giroux M,Maurage CA,Castellani V,Rebelo A,Abrams A,Z?chner S,Stojkovic T,Schaeffer L,Latour P

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NEFH cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

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