MYOD1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MYOD1
Entrez Gene ID	4654
Full Name	myogenic differentiation 1
Synonyms	MYF3,MYOD,PUM,bHLHc1
General protein information	Preferred Names myogenic differentiation 1 Names myoblast determination protein 1 class C basic helix-loop-helix protein 1 myf-3 myogenic factor 3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11p15.1
Summary	This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. [provided by RefSeq, Jul 2008].
Disorder MIM:	159970

mRNA and Protein(s)

mRNA	Protein	Name
NM_002478.4	NP_002469.2	myoblast determination protein 1
NM_002478.5	NP_002469.2	myoblast determination protein 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MYOD1	XP_508311.2
Canis lupus familiaris (dog)	MYOD1	XP_854756.1
Gallus gallus (chicken)	MYOD1	NP_989545.1
Danio rerio (zebrafish)	myod1	NP_571337.2
Xenopus tropicalis (tropical clawed frog)	myod1	NP_988972.1
	MYOD1	XP_001088038.1
Homo sapiens (human)	MYOD1	NP_002469.2
Bos taurus (cattle)	MYOD1	NP_001035568.2
Mus musculus (house mouse)	Myod1	NP_034996.2
Rattus norvegicus (Norway rat)	Myod1	NP_788268.1

Gene Ontology
Bibliography

Related articles in PubMed

ATP Citrate Lyase Regulates Myofiber Differentiation and Increases Regeneration by Altering Histone Acetylation.
Das S, Morvan F, Morozzi G, Jourde B, Minetti GC, Kahle P, Rivet H, Brebbia P, Toussaint G, Glass DJ, Fornaro M
Cell reports21(11)3003-3011(2017 Dec)

Incomplete MyoD-induced transdifferentiation is associated with chromatin remodeling deficiencies.
Manandhar D, Song L, Kabadi A, Kwon JB, Edsall LE, Ehrlich M, Tsumagari K, Gersbach CA, Crawford GE, Gord?n R
Nucleic acids research45(20)11684-11699(2017 Nov)

miRNA-223 upregulated by MYOD inhibits myoblast proliferation by repressing IGF2 and facilitates myoblast differentiation by inhibiting ZEB1.
Li G, Luo W, Abdalla BA, Ouyang H, Yu J, Hu F, Nie Q, Zhang X
Cell death & disease8(10)e3094(2017 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Cell transdifferentiation of primary skin fibroblasts by forced expression of myogenic transcription factor MyoD was performed by quantitative analyses of gene expression and chromatin accessibility profiles.
Title: Incomplete MyoD-induced transdifferentiation is associated with chromatin remodeling deficiencies.

The results strongly suggest that the combination of MYCL plus MYOD1 may promote direct conversion of human fibroblasts into functional myoblasts that could potentially be used for regenerative therapy for muscle diseases and congenital muscle defects.
Title: Efficient direct conversion of human fibroblasts into myogenic lineage induced by co-transduction with MYCL and MYOD1.

Data show that MeCP2 promotes gastric cancer (GC) cell proliferation via FOXF1-mediated Wnt5a/beta-Catenin signaling pathway, and suppresses GC cell apoptosis through MYOD1-mediated Caspase-3 signaling pathway.
Title: MeCP2 Promotes Gastric Cancer Progression Through Regulating FOXF1/Wnt5a/?-Catenin and MYOD1/Caspase-3 Signaling Pathways.

Analysis of human rhabdomyosarcoma revealed that MYF5 and MYOD are mutually-exclusively expressed and each is required for sustained tumor growth.
Title: Myogenic regulatory transcription factors regulate growth in rhabdomyosarcoma.

Analysis of the chromatin status of Cdkn1c promoter and KvDMR1 in unresponsive compared to responsive cell types showed that their differential responsiveness to the MyoD-dependent induction of the gene does not involve just their methylation status but, rather, the differential H3 lysine 9 dimethylation at KvDMR1.
Title: A cross-talk between DNA methylation and H3 lysine 9 dimethylation at the KvDMR1 region controls the induction of Cdkn1c in muscle cells.

The following MYOD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYOD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25217	NM_002478.5 Latest version!	Homo sapiens myogenic differentiation 1 (MYOD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
View Old Accession Versions >>
OHu25217	NM_002478.4	Homo sapiens myogenic differentiation 1 (MYOD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MYOD1 ( NM_002478.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002469.2 Homo sapiens myogenic differentiation 1 (MYOD1), mRNA.

Price & Availability↑

CloneID	OHu25217
Clone ID Related Accession (Same CDS sequence)	NM_002478.4 , NM_002478.5
Accession Version	NM_002478.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Homo sapiens(human)
Product	myoblast determination protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF304059.1, BF205042.1, AC124301.6 and AI300241.1. On Oct 14, 2005 this sequence version replaced NM_002478.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X56677.1, ERR279850.3240.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153307, SAMEA2162946 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCTAC TGTCGCCACC GCTCCGCGAC GTAGACCTGA CGGCCCCCGA CGGCTCTCTC 
TGCTCCTTTG CCACAACGGA CGACTTCTAT GACGACCCGT GTTTCGACTC CCCGGACCTG 
CGCTTCTTCG AAGACCTGGA CCCGCGCCTG ATGCACGTGG GCGCGCTCCT GAAACCCGAA 
GAGCACTCGC ACTTCCCCGC GGCGGTGCAC CCGGCCCCGG GCGCACGTGA GGACGAGCAT 
GTGCGCGCGC CCAGCGGGCA CCACCAGGCG GGCCGCTGCC TACTGTGGGC CTGCAAGGCG 
TGCAAGCGCA AGACCACCAA CGCCGACCGC CGCAAGGCCG CCACCATGCG CGAGCGGCGC 
CGCCTGAGCA AAGTAAATGA GGCCTTTGAG ACACTCAAGC GCTGCACGTC GAGCAATCCA 
AACCAGCGGT TGCCCAAGGT GGAGATCCTG CGCAACGCCA TCCGCTATAT CGAGGGCCTG 
CAGGCTCTGC TGCGCGACCA GGACGCCGCG CCCCCTGGCG CCGCAGCCGC CTTCTATGCG 
CCGGGCCCGC TGCCCCCGGG CCGCGGCGGC GAGCACTACA GCGGCGACTC CGACGCGTCC 
AGCCCGCGCT CCAACTGCTC CGACGGCATG ATGGACTACA GCGGCCCCCC GAGCGGCGCC 
CGGCGGCGGA ACTGCTACGA AGGCGCCTAC TACAACGAGG CGCCCAGCGA ACCCAGGCCC 
GGGAAGAGTG CGGCGGTGTC GAGCCTAGAC TGCCTGTCCA GCATCGTGGA GCGCATCTCC 
ACCGAGAGCC CTGCGGCGCC CGCCCTCCTG CTGGCGGACG TGCCTTCTGA GTCGCCTCCG 
CGCAGGCAAG AGGCTGCCGC CCCCAGCGAG GGAGAGAGCA GCGGCGACCC CACCCAGTCA 
CCGGACGCCG CCCCGCAGTG CCCTGCGGGT GCGAACCCCA ACCCGATATA CCAGGTGCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002469.2
CDS	221..1183
Translation	MELLSPPLRDVDLTAPDGSLCSFATTDDFYDDPCFDSPDLRFFEDLDPRLMHVGALLKPEEHSHFPAAVHPAPGAREDEHVRAPSGHHQAGRCLLWACKACKRKTTNADRRKAATMRERRRLSKVNEAFETLKRCTSSNPNQRLPKVEILRNAIRYIEGLQALLRDQDAAPPGAAAAFYAPGPLPPGRGGEHYSGDSDASSPRSNCSDGMMDYSGPPSGARRRNCYEGAYYNEAPSEPRPGKSAAVSSLDCLSSIVERISTESPAAPALLLADVPSESPPRRQEAAAPSEGESSGDPTQSPDAAPQCPAGANPNPIYQVL

Target ORF information:

RefSeq Version	NM_002478.4
Organism	Homo sapiens(human)
Definition	Homo sapiens myogenic differentiation 1 (MYOD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002478.4

ORF Insert Sequence:

ATGGAGCTAC TGTCGCCACC GCTCCGCGAC GTAGACCTGA CGGCCCCCGA CGGCTCTCTC 
TGCTCCTTTG CCACAACGGA CGACTTCTAT GACGACCCGT GTTTCGACTC CCCGGACCTG 
CGCTTCTTCG AAGACCTGGA CCCGCGCCTG ATGCACGTGG GCGCGCTCCT GAAACCCGAA 
GAGCACTCGC ACTTCCCCGC GGCGGTGCAC CCGGCCCCGG GCGCACGTGA GGACGAGCAT 
GTGCGCGCGC CCAGCGGGCA CCACCAGGCG GGCCGCTGCC TACTGTGGGC CTGCAAGGCG 
TGCAAGCGCA AGACCACCAA CGCCGACCGC CGCAAGGCCG CCACCATGCG CGAGCGGCGC 
CGCCTGAGCA AAGTAAATGA GGCCTTTGAG ACACTCAAGC GCTGCACGTC GAGCAATCCA 
AACCAGCGGT TGCCCAAGGT GGAGATCCTG CGCAACGCCA TCCGCTATAT CGAGGGCCTG 
CAGGCTCTGC TGCGCGACCA GGACGCCGCG CCCCCTGGCG CCGCAGCCGC CTTCTATGCG 
CCGGGCCCGC TGCCCCCGGG CCGCGGCGGC GAGCACTACA GCGGCGACTC CGACGCGTCC 
AGCCCGCGCT CCAACTGCTC CGACGGCATG ATGGACTACA GCGGCCCCCC GAGCGGCGCC 
CGGCGGCGGA ACTGCTACGA AGGCGCCTAC TACAACGAGG CGCCCAGCGA ACCCAGGCCC 
GGGAAGAGTG CGGCGGTGTC GAGCCTAGAC TGCCTGTCCA GCATCGTGGA GCGCATCTCC 
ACCGAGAGCC CTGCGGCGCC CGCCCTCCTG CTGGCGGACG TGCCTTCTGA GTCGCCTCCG 
CGCAGGCAAG AGGCTGCCGC CCCCAGCGAG GGAGAGAGCA GCGGCGACCC CACCCAGTCA 
CCGGACGCCG CCCCGCAGTG CCCTGCGGGT GCGAACCCCA ACCCGATATA CCAGGTGCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYOD1 ( NM_002478.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_002469.2 Homo sapiens myogenic differentiation 1 (MYOD1), mRNA.

Price & Availability↑

CloneID	OHu25217
Clone ID Related Accession (Same CDS sequence)	NM_002478.4 , NM_002478.5
Accession Version	NM_002478.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	myoblast determination protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF304059.1, BF205042.1, AC124301.6 and AI300241.1. On Nov 23, 2018 this sequence version replaced NM_002478.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X56677.1, X17650.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2153307, SAMEA2162946 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000250003.4/ ENSP00000250003.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGCTAC TGTCGCCACC GCTCCGCGAC GTAGACCTGA CGGCCCCCGA CGGCTCTCTC 
TGCTCCTTTG CCACAACGGA CGACTTCTAT GACGACCCGT GTTTCGACTC CCCGGACCTG 
CGCTTCTTCG AAGACCTGGA CCCGCGCCTG ATGCACGTGG GCGCGCTCCT GAAACCCGAA 
GAGCACTCGC ACTTCCCCGC GGCGGTGCAC CCGGCCCCGG GCGCACGTGA GGACGAGCAT 
GTGCGCGCGC CCAGCGGGCA CCACCAGGCG GGCCGCTGCC TACTGTGGGC CTGCAAGGCG 
TGCAAGCGCA AGACCACCAA CGCCGACCGC CGCAAGGCCG CCACCATGCG CGAGCGGCGC 
CGCCTGAGCA AAGTAAATGA GGCCTTTGAG ACACTCAAGC GCTGCACGTC GAGCAATCCA 
AACCAGCGGT TGCCCAAGGT GGAGATCCTG CGCAACGCCA TCCGCTATAT CGAGGGCCTG 
CAGGCTCTGC TGCGCGACCA GGACGCCGCG CCCCCTGGCG CCGCAGCCGC CTTCTATGCG 
CCGGGCCCGC TGCCCCCGGG CCGCGGCGGC GAGCACTACA GCGGCGACTC CGACGCGTCC 
AGCCCGCGCT CCAACTGCTC CGACGGCATG ATGGACTACA GCGGCCCCCC GAGCGGCGCC 
CGGCGGCGGA ACTGCTACGA AGGCGCCTAC TACAACGAGG CGCCCAGCGA ACCCAGGCCC 
GGGAAGAGTG CGGCGGTGTC GAGCCTAGAC TGCCTGTCCA GCATCGTGGA GCGCATCTCC 
ACCGAGAGCC CTGCGGCGCC CGCCCTCCTG CTGGCGGACG TGCCTTCTGA GTCGCCTCCG 
CGCAGGCAAG AGGCTGCCGC CCCCAGCGAG GGAGAGAGCA GCGGCGACCC CACCCAGTCA 
CCGGACGCCG CCCCGCAGTG CCCTGCGGGT GCGAACCCCA ACCCGATATA CCAGGTGCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002469.2
CDS	213..1175
Translation	MELLSPPLRDVDLTAPDGSLCSFATTDDFYDDPCFDSPDLRFFEDLDPRLMHVGALLKPEEHSHFPAAVHPAPGAREDEHVRAPSGHHQAGRCLLWACKACKRKTTNADRRKAATMRERRRLSKVNEAFETLKRCTSSNPNQRLPKVEILRNAIRYIEGLQALLRDQDAAPPGAAAAFYAPGPLPPGRGGEHYSGDSDASSPRSNCSDGMMDYSGPPSGARRRNCYEGAYYNEAPSEPRPGKSAAVSSLDCLSSIVERISTESPAAPALLLADVPSESPPRRQEAAAPSEGESSGDPTQSPDAAPQCPAGANPNPIYQVL

Target ORF information:

RefSeq Version	NM_002478.5
Organism	Homo sapiens(human)
Definition	Homo sapiens myogenic differentiation 1 (MYOD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002478.5

ORF Insert Sequence:

ATGGAGCTAC TGTCGCCACC GCTCCGCGAC GTAGACCTGA CGGCCCCCGA CGGCTCTCTC 
TGCTCCTTTG CCACAACGGA CGACTTCTAT GACGACCCGT GTTTCGACTC CCCGGACCTG 
CGCTTCTTCG AAGACCTGGA CCCGCGCCTG ATGCACGTGG GCGCGCTCCT GAAACCCGAA 
GAGCACTCGC ACTTCCCCGC GGCGGTGCAC CCGGCCCCGG GCGCACGTGA GGACGAGCAT 
GTGCGCGCGC CCAGCGGGCA CCACCAGGCG GGCCGCTGCC TACTGTGGGC CTGCAAGGCG 
TGCAAGCGCA AGACCACCAA CGCCGACCGC CGCAAGGCCG CCACCATGCG CGAGCGGCGC 
CGCCTGAGCA AAGTAAATGA GGCCTTTGAG ACACTCAAGC GCTGCACGTC GAGCAATCCA 
AACCAGCGGT TGCCCAAGGT GGAGATCCTG CGCAACGCCA TCCGCTATAT CGAGGGCCTG 
CAGGCTCTGC TGCGCGACCA GGACGCCGCG CCCCCTGGCG CCGCAGCCGC CTTCTATGCG 
CCGGGCCCGC TGCCCCCGGG CCGCGGCGGC GAGCACTACA GCGGCGACTC CGACGCGTCC 
AGCCCGCGCT CCAACTGCTC CGACGGCATG ATGGACTACA GCGGCCCCCC GAGCGGCGCC 
CGGCGGCGGA ACTGCTACGA AGGCGCCTAC TACAACGAGG CGCCCAGCGA ACCCAGGCCC 
GGGAAGAGTG CGGCGGTGTC GAGCCTAGAC TGCCTGTCCA GCATCGTGGA GCGCATCTCC 
ACCGAGAGCC CTGCGGCGCC CGCCCTCCTG CTGGCGGACG TGCCTTCTGA GTCGCCTCCG 
CGCAGGCAAG AGGCTGCCGC CCCCAGCGAG GGAGAGAGCA GCGGCGACCC CACCCAGTCA 
CCGGACGCCG CCCCGCAGTG CCCTGCGGGT GCGAACCCCA ACCCGATATA CCAGGTGCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ATP Citrate Lyase Regulates Myofiber Differentiation and Increases Regeneration by Altering Histone Acetylation.
Cell reports21(11)3003-3011(2017 Dec)
Das S,Morvan F,Morozzi G,Jourde B,Minetti GC,Kahle P,Rivet H,Brebbia P,Toussaint G,Glass DJ,Fornaro M

Incomplete MyoD-induced transdifferentiation is associated with chromatin remodeling deficiencies.
Nucleic acids research45(20)11684-11699(2017 Nov)
Manandhar D,Song L,Kabadi A,Kwon JB,Edsall LE,Ehrlich M,Tsumagari K,Gersbach CA,Crawford GE,Gord?n R

miRNA-223 upregulated by MYOD inhibits myoblast proliferation by repressing IGF2 and facilitates myoblast differentiation by inhibiting ZEB1.
Cell death & disease8(10)e3094(2017 Oct)
Li G,Luo W,Abdalla BA,Ouyang H,Yu J,Hu F,Nie Q,Zhang X