SH3RF3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SH3RF3
Entrez Gene ID	344558
Full Name	SH3 domain containing ring finger 3
Synonyms	POSH2,SH3MD4
General protein information	Preferred Names SH3 domain containing ring finger 3 Names SH3 domain-containing RING finger protein 3 SH3 multiple domains 4 plenty of SH3s-2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q13

mRNA and Protein(s)

mRNA	Protein	Name
NM_001099289.3	NP_001092759.1	E3 ubiquitin-protein ligase SH3RF3 precursor
NM_001099289.2	NP_001092759.1	E3 ubiquitin-protein ligase SH3RF3 precursor
XM_011511110.2	XP_011509412.1	E3 ubiquitin-protein ligase SH3RF3 isoform X2
XM_011511109.2	XP_011509411.1	E3 ubiquitin-protein ligase SH3RF3 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	SH3RF3	NP_001092759.1
	SH3RF3	XP_001084626.2
Canis lupus familiaris (dog)	SH3RF3	XP_005626027.1
Xenopus tropicalis (tropical clawed frog)	sh3rf3	XP_002937706.1
Bos taurus (cattle)	SH3RF3	XP_002691322.1
Rattus norvegicus (Norway rat)	Sh3rf3	XP_006256492.1
Gallus gallus (chicken)	SH3RF3	XP_416930.4
Pan troglodytes (chimpanzee)	SH3RF3	XP_525875.3
Mus musculus (house mouse)	Sh3rf3	NP_766376.2

Gene Ontology
Bibliography

Related articles in PubMed

POSH2 is a RING finger E3 ligase with Rac1 binding activity through a partial CRIB domain.
K?rkk?inen S, van der Linden M, Renkema GH
FEBS letters584(18)3867-72(2010 Sep)

Identification of preferred protein interactions by phage-display of the human Src homology-3 proteome.
K?rkk?inen S, Hiipakka M, Wang JH, Kleino I, V?h?-Jaakkola M, Renkema GH, Liss M, Wagner R, Saksela K
EMBO reports7(2)186-91(2006 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

POSH2 was found to have a conserved N-terminal extension not present in POSH1 and POSH3. POSH2 has GTP-loaded Rac1 binding activity and this activity was mapped to a partial Cdc42/Rac-interactive binding (CRIB) domain.
Title: POSH2 is a RING finger E3 ligase with Rac1 binding activity through a partial CRIB domain.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

The following SH3RF3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SH3RF3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu79444	XM_011511110.2 Latest version!	Homo sapiens SH3 domain containing ring finger 3 (SH3RF3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu14456	NM_001099289.3 Latest version!	Homo sapiens SH3 domain containing ring finger 3 (SH3RF3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OHu79443	XM_011511109.2 Latest version!	Homo sapiens SH3 domain containing ring finger 3 (SH3RF3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
View Old Accession Versions >>
OHu14456	NM_001099289.2	Homo sapiens SH3 domain containing ring finger 3 (SH3RF3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SH3RF3 ( XM_011511110.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509412.1 Homo sapiens SH3 domain containing ring finger 3 (SH3RF3), transcript variant X2, mRNA.

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CloneID	OHu79444
Clone ID Related Accession (Same CDS sequence)	XM_011511110.2
Accession Version	XM_011511110.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase SH3RF3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011511110.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAAGC CATGCCCAGC CGCTGCATCC AGCTGCAATG CCTCCCTGCC CTCTGACTCC 
GGCGCTGTGG CCAGCGTGGC CCCAAGTCCC ACTTTAAGCA GCTCAGGGGC GGTCAGTGCC 
TTTCAGCGGC GTGTGGATGG CAAGAAGAAC ACCAAGAAAC GCCACTCCTT CACCGCGCTC 
AGTGTGACGC ACAGATCCTC CCAGGCTGCC AGCCACAGGC ATTCCATGGA AATTAGTGCT 
CCAGTGTTGA TCAGCTCCAG CGATCCCCGA GCCGCGGCCA GGATTGGAGA CCTTGCTCAT 
CTGTCGTGCG CTGCTCCCAC CCAGGATGTC TCCTCCTCGG CGGGATCTAC CCCCACGGCT 
GTCCCACGGG CTGCCTCGGT GTCTGGAGAG CAGGGCACGC CTCCCAAGGT CCAGCTGCCC 
CTCAACGTGT ACCTGGCGCT CTACGCCTAC AAGCCCCAGA AGAGTGACGA GCTGGAGCTG 
CACAAGGGAG AGATGTACCG GGTCCTCGAG AAGTGTCAGG ATGGCTGGTT CAAGGGGGCG 
TCTCTGAGGA CCGGGGTCTC TGGGGTGTTC CCCGGAAACT ACGTGACACC CGTTTCCAGG 
GTGCCTGCAG GAGGGGCAGG GCCGCCCCGG AATAATGTAG TCGGAGGGTC TCCACTGGCC 
AAAGGGATAA CCACAACCAT GCACCCAGGC AGTGGGAGTC TGAGCAGCCT GGCCACTGCC 
ACCAGGCCCG CCCTGCCCAT CACCACTCCC CAGGCCCACG CCCAGCACCC CACAGCCTCG 
CCCCCAACAG GCAGCTGTCT ACGGCACTCA GCCCAGCCAA CGGCCAGCCA AGCCCGGAGC 
ACCATTTCAA CAGCTGCCCA CTCTGCAGCC CAGGCTCAGG ACCGGCCAAC TGCCACCGTG 
TCACCCCTGC GCACCCAGAA CTCTCCATCC CGCCTGCCTG CCACCAGCCT CAGGCCCCAC 
TCGGTGGTGT CCCCGCAGCA CAGCCACCAG CCCCCGGTGC AGATGTGCCC ACGGCCGGCC 
ATCCCCCTCA CATCAGCAGC ATCAGCCATC ACACCTCCCA ACGTCAGTGC CGCAAACCTC 
AACGGGGAGG CTGGAGGGGG GCCCATCGGT GTTCTGTCCA CATCCAGCCC CACCAACACG 
GGATGCAAAC TAGACGAGAA GAAAAGTGAA AAGAAAGAGA AGAAGAGTGG GCTCCTGAAG 
CTTCTAGCCG GAGCATCCAC CAAGAAGAAG TCACGCTCCC CGCCATCTGT GTCTCCAACC 
CACGACCCCC AGGTGGCCGT GGACGCCCTG CTCCAAGGTG CAGTGGGCCC CGAAGTGTCC 
TCACTGTCCA TCCACGGCAG GGCAGGGTCC TGCCCCATAG AGAGCGAGAT GCAGGGTGCC 
ATGGGGATGG AGCCTCTGCA CAGGAAGGCA GGCTCCTTGG ATCTAAACTT CACATCTCCT 
TCCCGGCAAG CTCCGCTGTC CATGGCTGCC ATCCGCCCCG AGCCCAAGCT GTTGCCCAGA 
GAGAGGTACC GCGTGGTGGT CTCGTACCCA CCCCAGAGTG AGGCGGAGAT CGAGCTGAAG 
GAAGGCGACA TCGTCTTTGT GCACAAGAAG CGTGAGGACG GCTGGTACAA GGGGACCCTG 
CAGCGGAACG GCCGCACAGG CCTCTTCCCG GGCAGCTTCG TCGAGAGCTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509412.1
CDS	778..2451
Translation	MDKPCPAAASSCNASLPSDSGAVASVAPSPTLSSSGAVSAFQRRVDGKKNTKKRHSFTALSVTHRSSQAASHRHSMEISAPVLISSSDPRAAARIGDLAHLSCAAPTQDVSSSAGSTPTAVPRAASVSGEQGTPPKVQLPLNVYLALYAYKPQKSDELELHKGEMYRVLEKCQDGWFKGASLRTGVSGVFPGNYVTPVSRVPAGGAGPPRNNVVGGSPLAKGITTTMHPGSGSLSSLATATRPALPITTPQAHAQHPTASPPTGSCLRHSAQPTASQARSTISTAAHSAAQAQDRPTATVSPLRTQNSPSRLPATSLRPHSVVSPQHSHQPPVQMCPRPAIPLTSAASAITPPNVSAANLNGEAGGGPIGVLSTSSPTNTGCKLDEKKSEKKEKKSGLLKLLAGASTKKKSRSPPSVSPTHDPQVAVDALLQGAVGPEVSSLSIHGRAGSCPIESEMQGAMGMEPLHRKAGSLDLNFTSPSRQAPLSMAAIRPEPKLLPRERYRVVVSYPPQSEAEIELKEGDIVFVHKKREDGWYKGTLQRNGRTGLFPGSFVESF

Target ORF information:

RefSeq Version	XM_011511110.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SH3 domain containing ring finger 3 (SH3RF3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511110.2

ORF Insert Sequence:

ATGGACAAGC CATGCCCAGC CGCTGCATCC AGCTGCAATG CCTCCCTGCC CTCTGACTCC 
GGCGCTGTGG CCAGCGTGGC CCCAAGTCCC ACTTTAAGCA GCTCAGGGGC GGTCAGTGCC 
TTTCAGCGGC GTGTGGATGG CAAGAAGAAC ACCAAGAAAC GCCACTCCTT CACCGCGCTC 
AGTGTGACGC ACAGATCCTC CCAGGCTGCC AGCCACAGGC ATTCCATGGA AATTAGTGCT 
CCAGTGTTGA TCAGCTCCAG CGATCCCCGA GCCGCGGCCA GGATTGGAGA CCTTGCTCAT 
CTGTCGTGCG CTGCTCCCAC CCAGGATGTC TCCTCCTCGG CGGGATCTAC CCCCACGGCT 
GTCCCACGGG CTGCCTCGGT GTCTGGAGAG CAGGGCACGC CTCCCAAGGT CCAGCTGCCC 
CTCAACGTGT ACCTGGCGCT CTACGCCTAC AAGCCCCAGA AGAGTGACGA GCTGGAGCTG 
CACAAGGGAG AGATGTACCG GGTCCTCGAG AAGTGTCAGG ATGGCTGGTT CAAGGGGGCG 
TCTCTGAGGA CCGGGGTCTC TGGGGTGTTC CCCGGAAACT ACGTGACACC CGTTTCCAGG 
GTGCCTGCAG GAGGGGCAGG GCCGCCCCGG AATAATGTAG TCGGAGGGTC TCCACTGGCC 
AAAGGGATAA CCACAACCAT GCACCCAGGC AGTGGGAGTC TGAGCAGCCT GGCCACTGCC 
ACCAGGCCCG CCCTGCCCAT CACCACTCCC CAGGCCCACG CCCAGCACCC CACAGCCTCG 
CCCCCAACAG GCAGCTGTCT ACGGCACTCA GCCCAGCCAA CGGCCAGCCA AGCCCGGAGC 
ACCATTTCAA CAGCTGCCCA CTCTGCAGCC CAGGCTCAGG ACCGGCCAAC TGCCACCGTG 
TCACCCCTGC GCACCCAGAA CTCTCCATCC CGCCTGCCTG CCACCAGCCT CAGGCCCCAC 
TCGGTGGTGT CCCCGCAGCA CAGCCACCAG CCCCCGGTGC AGATGTGCCC ACGGCCGGCC 
ATCCCCCTCA CATCAGCAGC ATCAGCCATC ACACCTCCCA ACGTCAGTGC CGCAAACCTC 
AACGGGGAGG CTGGAGGGGG GCCCATCGGT GTTCTGTCCA CATCCAGCCC CACCAACACG 
GGATGCAAAC TAGACGAGAA GAAAAGTGAA AAGAAAGAGA AGAAGAGTGG GCTCCTGAAG 
CTTCTAGCCG GAGCATCCAC CAAGAAGAAG TCACGCTCCC CGCCATCTGT GTCTCCAACC 
CACGACCCCC AGGTGGCCGT GGACGCCCTG CTCCAAGGTG CAGTGGGCCC CGAAGTGTCC 
TCACTGTCCA TCCACGGCAG GGCAGGGTCC TGCCCCATAG AGAGCGAGAT GCAGGGTGCC 
ATGGGGATGG AGCCTCTGCA CAGGAAGGCA GGCTCCTTGG ATCTAAACTT CACATCTCCT 
TCCCGGCAAG CTCCGCTGTC CATGGCTGCC ATCCGCCCCG AGCCCAAGCT GTTGCCCAGA 
GAGAGGTACC GCGTGGTGGT CTCGTACCCA CCCCAGAGTG AGGCGGAGAT CGAGCTGAAG 
GAAGGCGACA TCGTCTTTGT GCACAAGAAG CGTGAGGACG GCTGGTACAA GGGGACCCTG 
CAGCGGAACG GCCGCACAGG CCTCTTCCCG GGCAGCTTCG TCGAGAGCTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SH3RF3 ( NM_001099289.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001092759.1 Homo sapiens SH3 domain containing ring finger 3 (SH3RF3), mRNA.

Price & Availability↑

CloneID	OHu14456
Clone ID Related Accession (Same CDS sequence)	NM_001099289.3 , NM_001099289.2
Accession Version	NM_001099289.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2649bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase SH3RF3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC133109.3, DQ307286.1, DV080665.1, AK074131.1, HY224642.1 and AA916456.1. On May 17, 2019 this sequence version replaced NM_001099289.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology MANE Ensembl match :: ENST00000309415.8/ ENSP00000309186.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGCTCG GAGCGTCCTG GCTGTGCGCA TCCAAGGCGG CCGCCGCTGC TGCGCAGAGC 
GAGGGCGACG AGGACAGGCC AGGCGAGCGA CGGCGGCGTC GGGCGGCGGC CACCGCCGCG 
GGGGCGGGCG AGGACATGGA CGAGTCGTCG CTGCTGGACC TGCTGGAGTG CTCCGTGTGT 
CTGGAGCGCC TGGACACCAC GGCCAAGGTG CTGCCATGCC AACACACTTT CTGCCGCCGC 
TGCCTGGAGA GCATCGTGTG CTCGCGCCAC GAGCTGCGCT GCCCCGAGTG CCGCATCCTG 
GTGGGCTGCG GCGTGGACGA ACTGCCCGCC AACATCTTGC TGGTGCGACT GCTGGACGGC 
ATCCGTCAGC GGCCCCGCGC GGGCACCAGC CCCGGCGGCA GCCCGCCCGC GCGTCCCATC 
CCAGGCCAGA GTGCGGCCCC CACGCTCGCG GGCGGCGGGG GCGGCGCGGC AGGCAGCACC 
CCGGGTTCCC CGGTTTTCCT CTCCGCGGCC GCGGGCAGCA CCGCCGGCAG TCTGCGGGAG 
CTGGCGACCA GCAGGACCGC GCCGGCGGCA AAGAATCCCT GCCTGCTTCC CTATGGCAAG 
GCCCTCTACA GCTACGAGGG GAAGGAACCT GGTGACCTCA AGTTCAACAA GGGGGACATC 
ATCGTCCTGC GGCGCAAGGT GGATGAACAG TGGTACCACG GCGAGCTGCA CGGCACACAG 
GGCTTCCTCC CAGCCAGCTA TATCCAGTGC ATCCAGCCCT TGCCACACGC CCCGCCCCAG 
GGAAAAGCAC TTTATGATTT CGAGATGAAG GACAAAGACC AAGACAAGGA CTGTCTGACC 
TTCACCAAGG ACGAGATTCT GACGGTGCTC AGGAGAGTGG ATGAGAACTG GGCGGAAGGC 
ATGCTGGGAG ACAAGATCGG GATCTTCCCG CTCCTGTACG TGGAGCTCAA TGACTCCGCC 
AAGCAGCTCA TTGAGATGGA CAAGCCATGC CCAGCCGCTG CATCCAGCTG CAATGCCTCC 
CTGCCCTCTG ACTCCGGCGC TGTGGCCAGC GTGGCCCCAA GTCCCACTTT AAGCAGCTCA 
GGGGCGGTCA GTGCCTTTCA GCGGCGTGTG GATGGCAAGA AGAACACCAA GAAACGCCAC 
TCCTTCACCG CGCTCAGTGT GACGCACAGA TCCTCCCAGG CTGCCAGCCA CAGGCATTCC 
ATGGAAATTA GTGCTCCAGT GTTGATCAGC TCCAGCGATC CCCGAGCCGC GGCCAGGATT 
GGAGACCTTG CTCATCTGTC GTGCGCTGCT CCCACCCAGG ATGTCTCCTC CTCGGCGGGA 
TCTACCCCCA CGGCTGTCCC ACGGGCTGCC TCGGTGTCTG GAGAGCAGGG CACGCCTCCC 
AAGGTCCAGC TGCCCCTCAA CGTGTACCTG GCGCTCTACG CCTACAAGCC CCAGAAGAGT 
GACGAGCTGG AGCTGCACAA GGGAGAGATG TACCGGGTCC TCGAGAAGTG TCAGGATGGC 
TGGTTCAAGG GGGCGTCTCT GAGGACCGGG GTCTCTGGGG TGTTCCCCGG AAACTACGTG 
ACACCCGTTT CCAGGGTGCC TGCAGGAGGG GCAGGGCCGC CCCGGAATAA TGTAGTCGGA 
GGGTCTCCAC TGGCCAAAGG GATAACCACA ACCATGCACC CAGGCAGTGG GAGTCTGAGC 
AGCCTGGCCA CTGCCACCAG GCCCGCCCTG CCCATCACCA CTCCCCAGGC CCACGCCCAG 
CACCCCACAG CCTCGCCCCC AACAGGCAGC TGTCTACGGC ACTCAGCCCA GCCAACGGCC 
AGCCAAGCCC GGAGCACCAT TTCAACAGCT GCCCACTCTG CAGCCCAGGC TCAGGACCGG 
CCAACTGCCA CCGTGTCACC CCTGCGCACC CAGAACTCTC CATCCCGCCT GCCTGCCACC 
AGCCTCAGGC CCCACTCGGT GGTGTCCCCG CAGCACAGCC ACCAGCCCCC GGTGCAGATG 
TGCCCACGGC CGGCCATCCC CCTCACATCA GCAGCATCAG CCATCACACC TCCCAACGTC 
AGTGCCGCAA ACCTCAACGG GGAGGCTGGA GGGGGGCCCA TCGGTGTTCT GTCCACATCC 
AGCCCCACCA ACACGGGATG CAAACTAGAC GAGAAGAAAA GTGAAAAGAA AGAGAAGAAG 
AGTGGGCTCC TGAAGCTTCT AGCCGGAGCA TCCACCAAGA AGAAGTCACG CTCCCCGCCA 
TCTGTGTCTC CAACCCACGA CCCCCAGGTG GCCGTGGACG CCCTGCTCCA AGGTGCAGTG 
GGCCCCGAAG TGTCCTCACT GTCCATCCAC GGCAGGGCAG GGTCCTGCCC CATAGAGAGC 
GAGATGCAGG GTGCCATGGG GATGGAGCCT CTGCACAGGA AGGCAGGCTC CTTGGATCTA 
AACTTCACAT CTCCTTCCCG GCAAGCTCCG CTGTCCATGG CTGCCATCCG CCCCGAGCCC 
AAGCTGTTGC CCAGAGAGAG GTACCGCGTG GTGGTCTCGT ACCCACCCCA GAGTGAGGCG 
GAGATCGAGC TGAAGGAAGG CGACATCGTC TTTGTGCACA AGAAGCGTGA GGACGGCTGG 
TACAAGGGGA CCCTGCAGCG GAACGGCCGC ACAGGCCTCT TCCCGGGCAG CTTCGTCGAG 
AGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001092759.1
CDS	337..2985
Translation	MLLGASWLCASKAAAAAAQSEGDEDRPGERRRRRAAATAAGAGEDMDESSLLDLLECSVCLERLDTTAKVLPCQHTFCRRCLESIVCSRHELRCPECRILVGCGVDELPANILLVRLLDGIRQRPRAGTSPGGSPPARPIPGQSAAPTLAGGGGGAAGSTPGSPVFLSAAAGSTAGSLRELATSRTAPAAKNPCLLPYGKALYSYEGKEPGDLKFNKGDIIVLRRKVDEQWYHGELHGTQGFLPASYIQCIQPLPHAPPQGKALYDFEMKDKDQDKDCLTFTKDEILTVLRRVDENWAEGMLGDKIGIFPLLYVELNDSAKQLIEMDKPCPAAASSCNASLPSDSGAVASVAPSPTLSSSGAVSAFQRRVDGKKNTKKRHSFTALSVTHRSSQAASHRHSMEISAPVLISSSDPRAAARIGDLAHLSCAAPTQDVSSSAGSTPTAVPRAASVSGEQGTPPKVQLPLNVYLALYAYKPQKSDELELHKGEMYRVLEKCQDGWFKGASLRTGVSGVFPGNYVTPVSRVPAGGAGPPRNNVVGGSPLAKGITTTMHPGSGSLSSLATATRPALPITTPQAHAQHPTASPPTGSCLRHSAQPTASQARSTISTAAHSAAQAQDRPTATVSPLRTQNSPSRLPATSLRPHSVVSPQHSHQPPVQMCPRPAIPLTSAASAITPPNVSAANLNGEAGGGPIGVLSTSSPTNTGCKLDEKKSEKKEKKSGLLKLLAGASTKKKSRSPPSVSPTHDPQVAVDALLQGAVGPEVSSLSIHGRAGSCPIESEMQGAMGMEPLHRKAGSLDLNFTSPSRQAPLSMAAIRPEPKLLPRERYRVVVSYPPQSEAEIELKEGDIVFVHKKREDGWYKGTLQRNGRTGLFPGSFVESF

Target ORF information:

RefSeq Version	NM_001099289.3
Organism	Homo sapiens(human)
Definition	Homo sapiens SH3 domain containing ring finger 3 (SH3RF3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001099289.3

ORF Insert Sequence:

ATGCTGCTCG GAGCGTCCTG GCTGTGCGCA TCCAAGGCGG CCGCCGCTGC TGCGCAGAGC 
GAGGGCGACG AGGACAGGCC AGGCGAGCGA CGGCGGCGTC GGGCGGCGGC CACCGCCGCG 
GGGGCGGGCG AGGACATGGA CGAGTCGTCG CTGCTGGACC TGCTGGAGTG CTCCGTGTGT 
CTGGAGCGCC TGGACACCAC GGCCAAGGTG CTGCCATGCC AACACACTTT CTGCCGCCGC 
TGCCTGGAGA GCATCGTGTG CTCGCGCCAC GAGCTGCGCT GCCCCGAGTG CCGCATCCTG 
GTGGGCTGCG GCGTGGACGA ACTGCCCGCC AACATCTTGC TGGTGCGACT GCTGGACGGC 
ATCCGTCAGC GGCCCCGCGC GGGCACCAGC CCCGGCGGCA GCCCGCCCGC GCGTCCCATC 
CCAGGCCAGA GTGCGGCCCC CACGCTCGCG GGCGGCGGGG GCGGCGCGGC AGGCAGCACC 
CCGGGTTCCC CGGTTTTCCT CTCCGCGGCC GCGGGCAGCA CCGCCGGCAG TCTGCGGGAG 
CTGGCGACCA GCAGGACCGC GCCGGCGGCA AAGAATCCCT GCCTGCTTCC CTATGGCAAG 
GCCCTCTACA GCTACGAGGG GAAGGAACCT GGTGACCTCA AGTTCAACAA GGGGGACATC 
ATCGTCCTGC GGCGCAAGGT GGATGAACAG TGGTACCACG GCGAGCTGCA CGGCACACAG 
GGCTTCCTCC CAGCCAGCTA TATCCAGTGC ATCCAGCCCT TGCCACACGC CCCGCCCCAG 
GGAAAAGCAC TTTATGATTT CGAGATGAAG GACAAAGACC AAGACAAGGA CTGTCTGACC 
TTCACCAAGG ACGAGATTCT GACGGTGCTC AGGAGAGTGG ATGAGAACTG GGCGGAAGGC 
ATGCTGGGAG ACAAGATCGG GATCTTCCCG CTCCTGTACG TGGAGCTCAA TGACTCCGCC 
AAGCAGCTCA TTGAGATGGA CAAGCCATGC CCAGCCGCTG CATCCAGCTG CAATGCCTCC 
CTGCCCTCTG ACTCCGGCGC TGTGGCCAGC GTGGCCCCAA GTCCCACTTT AAGCAGCTCA 
GGGGCGGTCA GTGCCTTTCA GCGGCGTGTG GATGGCAAGA AGAACACCAA GAAACGCCAC 
TCCTTCACCG CGCTCAGTGT GACGCACAGA TCCTCCCAGG CTGCCAGCCA CAGGCATTCC 
ATGGAAATTA GTGCTCCAGT GTTGATCAGC TCCAGCGATC CCCGAGCCGC GGCCAGGATT 
GGAGACCTTG CTCATCTGTC GTGCGCTGCT CCCACCCAGG ATGTCTCCTC CTCGGCGGGA 
TCTACCCCCA CGGCTGTCCC ACGGGCTGCC TCGGTGTCTG GAGAGCAGGG CACGCCTCCC 
AAGGTCCAGC TGCCCCTCAA CGTGTACCTG GCGCTCTACG CCTACAAGCC CCAGAAGAGT 
GACGAGCTGG AGCTGCACAA GGGAGAGATG TACCGGGTCC TCGAGAAGTG TCAGGATGGC 
TGGTTCAAGG GGGCGTCTCT GAGGACCGGG GTCTCTGGGG TGTTCCCCGG AAACTACGTG 
ACACCCGTTT CCAGGGTGCC TGCAGGAGGG GCAGGGCCGC CCCGGAATAA TGTAGTCGGA 
GGGTCTCCAC TGGCCAAAGG GATAACCACA ACCATGCACC CAGGCAGTGG GAGTCTGAGC 
AGCCTGGCCA CTGCCACCAG GCCCGCCCTG CCCATCACCA CTCCCCAGGC CCACGCCCAG 
CACCCCACAG CCTCGCCCCC AACAGGCAGC TGTCTACGGC ACTCAGCCCA GCCAACGGCC 
AGCCAAGCCC GGAGCACCAT TTCAACAGCT GCCCACTCTG CAGCCCAGGC TCAGGACCGG 
CCAACTGCCA CCGTGTCACC CCTGCGCACC CAGAACTCTC CATCCCGCCT GCCTGCCACC 
AGCCTCAGGC CCCACTCGGT GGTGTCCCCG CAGCACAGCC ACCAGCCCCC GGTGCAGATG 
TGCCCACGGC CGGCCATCCC CCTCACATCA GCAGCATCAG CCATCACACC TCCCAACGTC 
AGTGCCGCAA ACCTCAACGG GGAGGCTGGA GGGGGGCCCA TCGGTGTTCT GTCCACATCC 
AGCCCCACCA ACACGGGATG CAAACTAGAC GAGAAGAAAA GTGAAAAGAA AGAGAAGAAG 
AGTGGGCTCC TGAAGCTTCT AGCCGGAGCA TCCACCAAGA AGAAGTCACG CTCCCCGCCA 
TCTGTGTCTC CAACCCACGA CCCCCAGGTG GCCGTGGACG CCCTGCTCCA AGGTGCAGTG 
GGCCCCGAAG TGTCCTCACT GTCCATCCAC GGCAGGGCAG GGTCCTGCCC CATAGAGAGC 
GAGATGCAGG GTGCCATGGG GATGGAGCCT CTGCACAGGA AGGCAGGCTC CTTGGATCTA 
AACTTCACAT CTCCTTCCCG GCAAGCTCCG CTGTCCATGG CTGCCATCCG CCCCGAGCCC 
AAGCTGTTGC CCAGAGAGAG GTACCGCGTG GTGGTCTCGT ACCCACCCCA GAGTGAGGCG 
GAGATCGAGC TGAAGGAAGG CGACATCGTC TTTGTGCACA AGAAGCGTGA GGACGGCTGG 
TACAAGGGGA CCCTGCAGCG GAACGGCCGC ACAGGCCTCT TCCCGGGCAG CTTCGTCGAG 
AGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SH3RF3 ( NM_001099289.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001092759.1 Homo sapiens SH3 domain containing ring finger 3 (SH3RF3), mRNA.

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CloneID	OHu14456
Clone ID Related Accession (Same CDS sequence)	NM_001099289.3 , NM_001099289.2
Accession Version	NM_001099289.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2649bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase SH3RF3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC133109.3, DQ307286.1, DV080665.1, AK074131.1, HY224642.1 and AA916456.1. On Apr 4, 2014 this sequence version replaced NM_001099289.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGCTCG GAGCGTCCTG GCTGTGCGCA TCCAAGGCGG CCGCCGCTGC TGCGCAGAGC 
GAGGGCGACG AGGACAGGCC AGGCGAGCGA CGGCGGCGTC GGGCGGCGGC CACCGCCGCG 
GGGGCGGGCG AGGACATGGA CGAGTCGTCG CTGCTGGACC TGCTGGAGTG CTCCGTGTGT 
CTGGAGCGCC TGGACACCAC GGCCAAGGTG CTGCCATGCC AACACACTTT CTGCCGCCGC 
TGCCTGGAGA GCATCGTGTG CTCGCGCCAC GAGCTGCGCT GCCCCGAGTG CCGCATCCTG 
GTGGGCTGCG GCGTGGACGA ACTGCCCGCC AACATCTTGC TGGTGCGACT GCTGGACGGC 
ATCCGTCAGC GGCCCCGCGC GGGCACCAGC CCCGGCGGCA GCCCGCCCGC GCGTCCCATC 
CCAGGCCAGA GTGCGGCCCC CACGCTCGCG GGCGGCGGGG GCGGCGCGGC AGGCAGCACC 
CCGGGTTCCC CGGTTTTCCT CTCCGCGGCC GCGGGCAGCA CCGCCGGCAG TCTGCGGGAG 
CTGGCGACCA GCAGGACCGC GCCGGCGGCA AAGAATCCCT GCCTGCTTCC CTATGGCAAG 
GCCCTCTACA GCTACGAGGG GAAGGAACCT GGTGACCTCA AGTTCAACAA GGGGGACATC 
ATCGTCCTGC GGCGCAAGGT GGATGAACAG TGGTACCACG GCGAGCTGCA CGGCACACAG 
GGCTTCCTCC CAGCCAGCTA TATCCAGTGC ATCCAGCCCT TGCCACACGC CCCGCCCCAG 
GGAAAAGCAC TTTATGATTT CGAGATGAAG GACAAAGACC AAGACAAGGA CTGTCTGACC 
TTCACCAAGG ACGAGATTCT GACGGTGCTC AGGAGAGTGG ATGAGAACTG GGCGGAAGGC 
ATGCTGGGAG ACAAGATCGG GATCTTCCCG CTCCTGTACG TGGAGCTCAA TGACTCCGCC 
AAGCAGCTCA TTGAGATGGA CAAGCCATGC CCAGCCGCTG CATCCAGCTG CAATGCCTCC 
CTGCCCTCTG ACTCCGGCGC TGTGGCCAGC GTGGCCCCAA GTCCCACTTT AAGCAGCTCA 
GGGGCGGTCA GTGCCTTTCA GCGGCGTGTG GATGGCAAGA AGAACACCAA GAAACGCCAC 
TCCTTCACCG CGCTCAGTGT GACGCACAGA TCCTCCCAGG CTGCCAGCCA CAGGCATTCC 
ATGGAAATTA GTGCTCCAGT GTTGATCAGC TCCAGCGATC CCCGAGCCGC GGCCAGGATT 
GGAGACCTTG CTCATCTGTC GTGCGCTGCT CCCACCCAGG ATGTCTCCTC CTCGGCGGGA 
TCTACCCCCA CGGCTGTCCC ACGGGCTGCC TCGGTGTCTG GAGAGCAGGG CACGCCTCCC 
AAGGTCCAGC TGCCCCTCAA CGTGTACCTG GCGCTCTACG CCTACAAGCC CCAGAAGAGT 
GACGAGCTGG AGCTGCACAA GGGAGAGATG TACCGGGTCC TCGAGAAGTG TCAGGATGGC 
TGGTTCAAGG GGGCGTCTCT GAGGACCGGG GTCTCTGGGG TGTTCCCCGG AAACTACGTG 
ACACCCGTTT CCAGGGTGCC TGCAGGAGGG GCAGGGCCGC CCCGGAATAA TGTAGTCGGA 
GGGTCTCCAC TGGCCAAAGG GATAACCACA ACCATGCACC CAGGCAGTGG GAGTCTGAGC 
AGCCTGGCCA CTGCCACCAG GCCCGCCCTG CCCATCACCA CTCCCCAGGC CCACGCCCAG 
CACCCCACAG CCTCGCCCCC AACAGGCAGC TGTCTACGGC ACTCAGCCCA GCCAACGGCC 
AGCCAAGCCC GGAGCACCAT TTCAACAGCT GCCCACTCTG CAGCCCAGGC TCAGGACCGG 
CCAACTGCCA CCGTGTCACC CCTGCGCACC CAGAACTCTC CATCCCGCCT GCCTGCCACC 
AGCCTCAGGC CCCACTCGGT GGTGTCCCCG CAGCACAGCC ACCAGCCCCC GGTGCAGATG 
TGCCCACGGC CGGCCATCCC CCTCACATCA GCAGCATCAG CCATCACACC TCCCAACGTC 
AGTGCCGCAA ACCTCAACGG GGAGGCTGGA GGGGGGCCCA TCGGTGTTCT GTCCACATCC 
AGCCCCACCA ACACGGGATG CAAACTAGAC GAGAAGAAAA GTGAAAAGAA AGAGAAGAAG 
AGTGGGCTCC TGAAGCTTCT AGCCGGAGCA TCCACCAAGA AGAAGTCACG CTCCCCGCCA 
TCTGTGTCTC CAACCCACGA CCCCCAGGTG GCCGTGGACG CCCTGCTCCA AGGTGCAGTG 
GGCCCCGAAG TGTCCTCACT GTCCATCCAC GGCAGGGCAG GGTCCTGCCC CATAGAGAGC 
GAGATGCAGG GTGCCATGGG GATGGAGCCT CTGCACAGGA AGGCAGGCTC CTTGGATCTA 
AACTTCACAT CTCCTTCCCG GCAAGCTCCG CTGTCCATGG CTGCCATCCG CCCCGAGCCC 
AAGCTGTTGC CCAGAGAGAG GTACCGCGTG GTGGTCTCGT ACCCACCCCA GAGTGAGGCG 
GAGATCGAGC TGAAGGAAGG CGACATCGTC TTTGTGCACA AGAAGCGTGA GGACGGCTGG 
TACAAGGGGA CCCTGCAGCG GAACGGCCGC ACAGGCCTCT TCCCGGGCAG CTTCGTCGAG 
AGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001092759.1
CDS	1..2649
Translation	MLLGASWLCASKAAAAAAQSEGDEDRPGERRRRRAAATAAGAGEDMDESSLLDLLECSVCLERLDTTAKVLPCQHTFCRRCLESIVCSRHELRCPECRILVGCGVDELPANILLVRLLDGIRQRPRAGTSPGGSPPARPIPGQSAAPTLAGGGGGAAGSTPGSPVFLSAAAGSTAGSLRELATSRTAPAAKNPCLLPYGKALYSYEGKEPGDLKFNKGDIIVLRRKVDEQWYHGELHGTQGFLPASYIQCIQPLPHAPPQGKALYDFEMKDKDQDKDCLTFTKDEILTVLRRVDENWAEGMLGDKIGIFPLLYVELNDSAKQLIEMDKPCPAAASSCNASLPSDSGAVASVAPSPTLSSSGAVSAFQRRVDGKKNTKKRHSFTALSVTHRSSQAASHRHSMEISAPVLISSSDPRAAARIGDLAHLSCAAPTQDVSSSAGSTPTAVPRAASVSGEQGTPPKVQLPLNVYLALYAYKPQKSDELELHKGEMYRVLEKCQDGWFKGASLRTGVSGVFPGNYVTPVSRVPAGGAGPPRNNVVGGSPLAKGITTTMHPGSGSLSSLATATRPALPITTPQAHAQHPTASPPTGSCLRHSAQPTASQARSTISTAAHSAAQAQDRPTATVSPLRTQNSPSRLPATSLRPHSVVSPQHSHQPPVQMCPRPAIPLTSAASAITPPNVSAANLNGEAGGGPIGVLSTSSPTNTGCKLDEKKSEKKEKKSGLLKLLAGASTKKKSRSPPSVSPTHDPQVAVDALLQGAVGPEVSSLSIHGRAGSCPIESEMQGAMGMEPLHRKAGSLDLNFTSPSRQAPLSMAAIRPEPKLLPRERYRVVVSYPPQSEAEIELKEGDIVFVHKKREDGWYKGTLQRNGRTGLFPGSFVESF

Target ORF information:

RefSeq Version	NM_001099289.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SH3 domain containing ring finger 3 (SH3RF3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001099289.2

ORF Insert Sequence:

ATGCTGCTCG GAGCGTCCTG GCTGTGCGCA TCCAAGGCGG CCGCCGCTGC TGCGCAGAGC 
GAGGGCGACG AGGACAGGCC AGGCGAGCGA CGGCGGCGTC GGGCGGCGGC CACCGCCGCG 
GGGGCGGGCG AGGACATGGA CGAGTCGTCG CTGCTGGACC TGCTGGAGTG CTCCGTGTGT 
CTGGAGCGCC TGGACACCAC GGCCAAGGTG CTGCCATGCC AACACACTTT CTGCCGCCGC 
TGCCTGGAGA GCATCGTGTG CTCGCGCCAC GAGCTGCGCT GCCCCGAGTG CCGCATCCTG 
GTGGGCTGCG GCGTGGACGA ACTGCCCGCC AACATCTTGC TGGTGCGACT GCTGGACGGC 
ATCCGTCAGC GGCCCCGCGC GGGCACCAGC CCCGGCGGCA GCCCGCCCGC GCGTCCCATC 
CCAGGCCAGA GTGCGGCCCC CACGCTCGCG GGCGGCGGGG GCGGCGCGGC AGGCAGCACC 
CCGGGTTCCC CGGTTTTCCT CTCCGCGGCC GCGGGCAGCA CCGCCGGCAG TCTGCGGGAG 
CTGGCGACCA GCAGGACCGC GCCGGCGGCA AAGAATCCCT GCCTGCTTCC CTATGGCAAG 
GCCCTCTACA GCTACGAGGG GAAGGAACCT GGTGACCTCA AGTTCAACAA GGGGGACATC 
ATCGTCCTGC GGCGCAAGGT GGATGAACAG TGGTACCACG GCGAGCTGCA CGGCACACAG 
GGCTTCCTCC CAGCCAGCTA TATCCAGTGC ATCCAGCCCT TGCCACACGC CCCGCCCCAG 
GGAAAAGCAC TTTATGATTT CGAGATGAAG GACAAAGACC AAGACAAGGA CTGTCTGACC 
TTCACCAAGG ACGAGATTCT GACGGTGCTC AGGAGAGTGG ATGAGAACTG GGCGGAAGGC 
ATGCTGGGAG ACAAGATCGG GATCTTCCCG CTCCTGTACG TGGAGCTCAA TGACTCCGCC 
AAGCAGCTCA TTGAGATGGA CAAGCCATGC CCAGCCGCTG CATCCAGCTG CAATGCCTCC 
CTGCCCTCTG ACTCCGGCGC TGTGGCCAGC GTGGCCCCAA GTCCCACTTT AAGCAGCTCA 
GGGGCGGTCA GTGCCTTTCA GCGGCGTGTG GATGGCAAGA AGAACACCAA GAAACGCCAC 
TCCTTCACCG CGCTCAGTGT GACGCACAGA TCCTCCCAGG CTGCCAGCCA CAGGCATTCC 
ATGGAAATTA GTGCTCCAGT GTTGATCAGC TCCAGCGATC CCCGAGCCGC GGCCAGGATT 
GGAGACCTTG CTCATCTGTC GTGCGCTGCT CCCACCCAGG ATGTCTCCTC CTCGGCGGGA 
TCTACCCCCA CGGCTGTCCC ACGGGCTGCC TCGGTGTCTG GAGAGCAGGG CACGCCTCCC 
AAGGTCCAGC TGCCCCTCAA CGTGTACCTG GCGCTCTACG CCTACAAGCC CCAGAAGAGT 
GACGAGCTGG AGCTGCACAA GGGAGAGATG TACCGGGTCC TCGAGAAGTG TCAGGATGGC 
TGGTTCAAGG GGGCGTCTCT GAGGACCGGG GTCTCTGGGG TGTTCCCCGG AAACTACGTG 
ACACCCGTTT CCAGGGTGCC TGCAGGAGGG GCAGGGCCGC CCCGGAATAA TGTAGTCGGA 
GGGTCTCCAC TGGCCAAAGG GATAACCACA ACCATGCACC CAGGCAGTGG GAGTCTGAGC 
AGCCTGGCCA CTGCCACCAG GCCCGCCCTG CCCATCACCA CTCCCCAGGC CCACGCCCAG 
CACCCCACAG CCTCGCCCCC AACAGGCAGC TGTCTACGGC ACTCAGCCCA GCCAACGGCC 
AGCCAAGCCC GGAGCACCAT TTCAACAGCT GCCCACTCTG CAGCCCAGGC TCAGGACCGG 
CCAACTGCCA CCGTGTCACC CCTGCGCACC CAGAACTCTC CATCCCGCCT GCCTGCCACC 
AGCCTCAGGC CCCACTCGGT GGTGTCCCCG CAGCACAGCC ACCAGCCCCC GGTGCAGATG 
TGCCCACGGC CGGCCATCCC CCTCACATCA GCAGCATCAG CCATCACACC TCCCAACGTC 
AGTGCCGCAA ACCTCAACGG GGAGGCTGGA GGGGGGCCCA TCGGTGTTCT GTCCACATCC 
AGCCCCACCA ACACGGGATG CAAACTAGAC GAGAAGAAAA GTGAAAAGAA AGAGAAGAAG 
AGTGGGCTCC TGAAGCTTCT AGCCGGAGCA TCCACCAAGA AGAAGTCACG CTCCCCGCCA 
TCTGTGTCTC CAACCCACGA CCCCCAGGTG GCCGTGGACG CCCTGCTCCA AGGTGCAGTG 
GGCCCCGAAG TGTCCTCACT GTCCATCCAC GGCAGGGCAG GGTCCTGCCC CATAGAGAGC 
GAGATGCAGG GTGCCATGGG GATGGAGCCT CTGCACAGGA AGGCAGGCTC CTTGGATCTA 
AACTTCACAT CTCCTTCCCG GCAAGCTCCG CTGTCCATGG CTGCCATCCG CCCCGAGCCC 
AAGCTGTTGC CCAGAGAGAG GTACCGCGTG GTGGTCTCGT ACCCACCCCA GAGTGAGGCG 
GAGATCGAGC TGAAGGAAGG CGACATCGTC TTTGTGCACA AGAAGCGTGA GGACGGCTGG 
TACAAGGGGA CCCTGCAGCG GAACGGCCGC ACAGGCCTCT TCCCGGGCAG CTTCGTCGAG 
AGCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SH3RF3 ( XM_011511109.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509411.1 Homo sapiens SH3 domain containing ring finger 3 (SH3RF3), transcript variant X1, mRNA.

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CloneID	OHu79443
Clone ID Related Accession (Same CDS sequence)	XM_011511109.2
Accession Version	XM_011511109.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2442bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase SH3RF3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011511109.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTCG GAGCGTCCTG GCTGTGCGCA TCCAAGGCGG CCGCCGCTGC TGCGCAGAGC 
GAGGGCGACG AGGACAGGCC AGGCGAGCGA CGGCGGCGTC GGGCGGCGGC CACCGCCGCG 
GGGGCGGGCG AGGACATGGA CGAGTCGTCG CTGCTGGACC TGCTGGAGTG CTCCGTGTGT 
CTGGAGCGCC TGGACACCAC GGCCAAGGTG CTGCCATGCC AACACACTTT CTGCCGCCGC 
TGCCTGGAGA GCATCGTGTG CTCGCGCCAC GAGCTGCGCT GCCCCGAGTG CCGCATCCTG 
GTGGGCTGCG GCGTGGACGA ACTGCCCGCC AACATCTTGC TGGTGCGACT GCTGGACGGC 
ATCCGTCAGC GGCCCCGCGC GGGCACCAGC CCCGGCGGCA GCCCGCCCGC GCGTCCCATC 
CCAGGCCAGA GTGCGGCCCC CACGCTCGCG GGCGGCGGGG GCGGCGCGGC AGGCAGCACC 
CCGGGTTCCC CGGTTTTCCT CTCCGCGGCC GCGGGCAGCA CCGCCGGCAG TCTGCGGGAG 
CTGGCGACCA GCAGGACCGC GCCGGCGGCA AAGAATCCCT GCCTGCTTCC CTATGGCAAG 
GCCCTCTACA GCTACGAGGG GAAGGAACCT GGTGACCTCA AGTTCAACAA GGGGGACATC 
ATCGTCCTGC GGCGCAAGGT GGATGAACAG TGGTACCACG GCGAGCTGCA CGGCACACAG 
GGCTTCCTCC CAGCCAGCTA TATCCAGTGC ATCCAGCCCT TGCCACACGC CCCGCCCCAG 
GGAAAAGCAC TTTATGATTT CGAGATGAAG GACAAAGACC AAGACAAGGA CTGTCTGACC 
TTCACCAAGG ACGAGATTCT GACGGTGCTC AGGAGAGTGG ATGAGAACTG GGCGGAAGGC 
ATGCTGGGAG ACAAGATCGG GATCTTCCCG CTCCTGTACG TGGAGCTCAA TGACTCCGCC 
AAGCAGCTCA TTGAGATGGA CAAGCCATGC CCAGCCGCTG CATCCAGCTG CAATGCCTCC 
CTGCCCTCTG ACTCCGGCGC TGTGGCCAGC GTGGCCCCAA GTCCCACTTT AAGCAGCTCA 
GGGGCGGTCA GTGCCTTTCA GCGGCGTGTG GATGGCAAGA AGAACACCAA GAAACGCCAC 
TCCTTCACCG CGCTCAGTGT GACGCACAGA TCCTCCCAGG CTGCCAGCCA CAGGCATTCC 
ATGGAAATTA GTGCTCCAGT GTTGATCAGC TCCAGCGATC CCCGAGCCGC GGCCAGGATT 
GGAGACCTTG CTCATCTGTC GTGCGCTGCT CCCACCCAGG ATGTCTCCTC CTCGGCGGGA 
TCTACCCCCA CGGCTGTCCC ACGGGCTGCC TCGGTGTCTG GAGAGCAGGG CACGCCTCCC 
AAGGTCCAGC TGCCCCTCAA CGTGTACCTG GCGCTCTACG CCTACAAGCC CCAGAAGAGT 
GACGAGCTGG AGCTGCACAA GGGAGAGATG TACCGGGTCC TCGAGAAGTG TCAGGATGGC 
TGGTTCAAGG GGGCGTCTCT GAGGACCGGG GTCTCTGGGG TGTTCCCCGG AAACTACGTG 
ACACCCGTTT CCAGGGTGCC TGCAGGAGGG GCAGGGCCGC CCCGGAATAA TGTAGTCGGA 
GGGTCTCCAC TGGCCAAAGG GATAACCACA ACCATGCACC CAGGCAGTGG GAGTCTGAGC 
AGCCTGGCCA CTGCCACCAG GCCCGCCCTG CCCATCACCA CTCCCCAGGC CCACGCCCAG 
CACCCCACAG CCTCGCCCCC AACAGGCAGC TGTCTACGGC ACTCAGCCCA GCCAACGGCC 
AGCCAAGCCC GGAGCACCAT TTCAACAGCT GCCCACTCTG CAGCCCAGGC TCAGGACCGG 
CCAACTGCCA CCGTGTCACC CCTGCGCACC CAGAACTCTC CATCCCGCCT GCCTGCCACC 
AGCCTCAGGC CCCACTCGGT GGTGTCCCCG CAGCACAGCC ACCAGCCCCC GGTGCAGATG 
TGCCCACGGC CGGCCATCCC CCTCACATCA GCAGCATCAG CCATCACACC TCCCAACGTC 
AGTGCCGCAA ACCTCAACGG GGAGGCTGGA GGGGGGCCCA TCGGTGTTCT GTCCACATCC 
AGCCCCACCA ACACGGGATG CAAACTAGAC GAGAAGAAAA GTGAAAAGAC CCCCAAAACC 
ATCCACAGAC CACTGATGAA GACCCCGGGA CTCACTGCCC TCAGAGCCTT CATACGGCAG 
CACCTAACCC ACCTGCAGCA CGAGGATCTC CCTGGAGCTC TTGCTCCAGC CCAGCCCGGT 
TTTGCTGACA GCCCCTCTGC AGCCCAGTGG TATGCAGGTG ACATGTCCCC TGAGGCCACG 
CGTCATCACA GCCTGAGGGA AGCAGTGGTT TCAACCCTGC TGTGGGTTGA GGACAATTGT 
CTAGAGGAAA GGACCCCATC CTGGCACTTC TCCTTTAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509411.1
CDS	103..2544
Translation	MLLGASWLCASKAAAAAAQSEGDEDRPGERRRRRAAATAAGAGEDMDESSLLDLLECSVCLERLDTTAKVLPCQHTFCRRCLESIVCSRHELRCPECRILVGCGVDELPANILLVRLLDGIRQRPRAGTSPGGSPPARPIPGQSAAPTLAGGGGGAAGSTPGSPVFLSAAAGSTAGSLRELATSRTAPAAKNPCLLPYGKALYSYEGKEPGDLKFNKGDIIVLRRKVDEQWYHGELHGTQGFLPASYIQCIQPLPHAPPQGKALYDFEMKDKDQDKDCLTFTKDEILTVLRRVDENWAEGMLGDKIGIFPLLYVELNDSAKQLIEMDKPCPAAASSCNASLPSDSGAVASVAPSPTLSSSGAVSAFQRRVDGKKNTKKRHSFTALSVTHRSSQAASHRHSMEISAPVLISSSDPRAAARIGDLAHLSCAAPTQDVSSSAGSTPTAVPRAASVSGEQGTPPKVQLPLNVYLALYAYKPQKSDELELHKGEMYRVLEKCQDGWFKGASLRTGVSGVFPGNYVTPVSRVPAGGAGPPRNNVVGGSPLAKGITTTMHPGSGSLSSLATATRPALPITTPQAHAQHPTASPPTGSCLRHSAQPTASQARSTISTAAHSAAQAQDRPTATVSPLRTQNSPSRLPATSLRPHSVVSPQHSHQPPVQMCPRPAIPLTSAASAITPPNVSAANLNGEAGGGPIGVLSTSSPTNTGCKLDEKKSEKTPKTIHRPLMKTPGLTALRAFIRQHLTHLQHEDLPGALAPAQPGFADSPSAAQWYAGDMSPEATRHHSLREAVVSTLLWVEDNCLEERTPSWHFSFS

Target ORF information:

RefSeq Version	XM_011511109.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SH3 domain containing ring finger 3 (SH3RF3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511109.2

ORF Insert Sequence:

ATGCTGCTCG GAGCGTCCTG GCTGTGCGCA TCCAAGGCGG CCGCCGCTGC TGCGCAGAGC 
GAGGGCGACG AGGACAGGCC AGGCGAGCGA CGGCGGCGTC GGGCGGCGGC CACCGCCGCG 
GGGGCGGGCG AGGACATGGA CGAGTCGTCG CTGCTGGACC TGCTGGAGTG CTCCGTGTGT 
CTGGAGCGCC TGGACACCAC GGCCAAGGTG CTGCCATGCC AACACACTTT CTGCCGCCGC 
TGCCTGGAGA GCATCGTGTG CTCGCGCCAC GAGCTGCGCT GCCCCGAGTG CCGCATCCTG 
GTGGGCTGCG GCGTGGACGA ACTGCCCGCC AACATCTTGC TGGTGCGACT GCTGGACGGC 
ATCCGTCAGC GGCCCCGCGC GGGCACCAGC CCCGGCGGCA GCCCGCCCGC GCGTCCCATC 
CCAGGCCAGA GTGCGGCCCC CACGCTCGCG GGCGGCGGGG GCGGCGCGGC AGGCAGCACC 
CCGGGTTCCC CGGTTTTCCT CTCCGCGGCC GCGGGCAGCA CCGCCGGCAG TCTGCGGGAG 
CTGGCGACCA GCAGGACCGC GCCGGCGGCA AAGAATCCCT GCCTGCTTCC CTATGGCAAG 
GCCCTCTACA GCTACGAGGG GAAGGAACCT GGTGACCTCA AGTTCAACAA GGGGGACATC 
ATCGTCCTGC GGCGCAAGGT GGATGAACAG TGGTACCACG GCGAGCTGCA CGGCACACAG 
GGCTTCCTCC CAGCCAGCTA TATCCAGTGC ATCCAGCCCT TGCCACACGC CCCGCCCCAG 
GGAAAAGCAC TTTATGATTT CGAGATGAAG GACAAAGACC AAGACAAGGA CTGTCTGACC 
TTCACCAAGG ACGAGATTCT GACGGTGCTC AGGAGAGTGG ATGAGAACTG GGCGGAAGGC 
ATGCTGGGAG ACAAGATCGG GATCTTCCCG CTCCTGTACG TGGAGCTCAA TGACTCCGCC 
AAGCAGCTCA TTGAGATGGA CAAGCCATGC CCAGCCGCTG CATCCAGCTG CAATGCCTCC 
CTGCCCTCTG ACTCCGGCGC TGTGGCCAGC GTGGCCCCAA GTCCCACTTT AAGCAGCTCA 
GGGGCGGTCA GTGCCTTTCA GCGGCGTGTG GATGGCAAGA AGAACACCAA GAAACGCCAC 
TCCTTCACCG CGCTCAGTGT GACGCACAGA TCCTCCCAGG CTGCCAGCCA CAGGCATTCC 
ATGGAAATTA GTGCTCCAGT GTTGATCAGC TCCAGCGATC CCCGAGCCGC GGCCAGGATT 
GGAGACCTTG CTCATCTGTC GTGCGCTGCT CCCACCCAGG ATGTCTCCTC CTCGGCGGGA 
TCTACCCCCA CGGCTGTCCC ACGGGCTGCC TCGGTGTCTG GAGAGCAGGG CACGCCTCCC 
AAGGTCCAGC TGCCCCTCAA CGTGTACCTG GCGCTCTACG CCTACAAGCC CCAGAAGAGT 
GACGAGCTGG AGCTGCACAA GGGAGAGATG TACCGGGTCC TCGAGAAGTG TCAGGATGGC 
TGGTTCAAGG GGGCGTCTCT GAGGACCGGG GTCTCTGGGG TGTTCCCCGG AAACTACGTG 
ACACCCGTTT CCAGGGTGCC TGCAGGAGGG GCAGGGCCGC CCCGGAATAA TGTAGTCGGA 
GGGTCTCCAC TGGCCAAAGG GATAACCACA ACCATGCACC CAGGCAGTGG GAGTCTGAGC 
AGCCTGGCCA CTGCCACCAG GCCCGCCCTG CCCATCACCA CTCCCCAGGC CCACGCCCAG 
CACCCCACAG CCTCGCCCCC AACAGGCAGC TGTCTACGGC ACTCAGCCCA GCCAACGGCC 
AGCCAAGCCC GGAGCACCAT TTCAACAGCT GCCCACTCTG CAGCCCAGGC TCAGGACCGG 
CCAACTGCCA CCGTGTCACC CCTGCGCACC CAGAACTCTC CATCCCGCCT GCCTGCCACC 
AGCCTCAGGC CCCACTCGGT GGTGTCCCCG CAGCACAGCC ACCAGCCCCC GGTGCAGATG 
TGCCCACGGC CGGCCATCCC CCTCACATCA GCAGCATCAG CCATCACACC TCCCAACGTC 
AGTGCCGCAA ACCTCAACGG GGAGGCTGGA GGGGGGCCCA TCGGTGTTCT GTCCACATCC 
AGCCCCACCA ACACGGGATG CAAACTAGAC GAGAAGAAAA GTGAAAAGAC CCCCAAAACC 
ATCCACAGAC CACTGATGAA GACCCCGGGA CTCACTGCCC TCAGAGCCTT CATACGGCAG 
CACCTAACCC ACCTGCAGCA CGAGGATCTC CCTGGAGCTC TTGCTCCAGC CCAGCCCGGT 
TTTGCTGACA GCCCCTCTGC AGCCCAGTGG TATGCAGGTG ACATGTCCCC TGAGGCCACG 
CGTCATCACA GCCTGAGGGA AGCAGTGGTT TCAACCCTGC TGTGGGTTGA GGACAATTGT 
CTAGAGGAAA GGACCCCATC CTGGCACTTC TCCTTTAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

POSH2 is a RING finger E3 ligase with Rac1 binding activity through a partial CRIB domain.
FEBS letters584(18)3867-72(2010 Sep)
K?rkk?inen S,van der Linden M,Renkema GH

Identification of preferred protein interactions by phage-display of the human Src homology-3 proteome.
EMBO reports7(2)186-91(2006 Feb)
K?rkk?inen S,Hiipakka M,Wang JH,Kleino I,V?h?-Jaakkola M,Renkema GH,Liss M,Wagner R,Saksela K

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SH3RF3 cDNA ORF clone, Homo sapiens(human)

Gene

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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