NANOS2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NANOS2
Entrez Gene ID	339345
Full Name	nanos C2HC-type zinc finger 2
Synonyms	NOS2,ZC2HC12B
General protein information	Preferred Names nanos C2HC-type zinc finger 2 Names nanos homolog 2 NOS-2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.32
Disorder MIM:	608228

mRNA and Protein(s)

mRNA	Protein	Name
NM_001029861.2	NP_001025032.1	nanos homolog 2
NM_001029861.3	NP_001025032.1	nanos homolog 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	NANOS2	NP_001025032.1
	NANOS2	XP_001111556.1
Canis lupus familiaris (dog)	NANOS2	XP_541547.1
Bos taurus (cattle)	NANOS2	XP_002695145.1
Mus musculus (house mouse)	Nanos2	NP_918953.2
Pan troglodytes (chimpanzee)	NANOS2	XP_001167172.1
Rattus norvegicus (Norway rat)	Nanos2	NP_001102378.1

Gene Ontology
Bibliography

Related articles in PubMed

Pluripotency induction in HEK293T cells by concurrent expression of STELLA, OCT4 and NANOS2.
Farshchian M, Matin MM, Bahrami M, Geerts D, Dastpak M, Shahriyari M, Tajeran M, Mirahmadi M, Bahrami AR
Biochemical and biophysical research communications480(4)635-640(2016 Nov)

Structural basis for the Nanos-mediated recruitment of the CCR4-NOT complex and translational repression.
Bhandari D, Raisch T, Weichenrieder O, Jonas S, Izaurralde E
Genes & development28(8)888-901(2014 Apr)

Analysis of meiosis regulators in human gonads: a sexually dimorphic spatio-temporal expression pattern suggests involvement of DMRT1 in meiotic entry.
J?rgensen A, Nielsen JE, Blomberg Jensen M, Gr?m N, Rajpert-De Meyts E
Molecular human reproduction18(11)523-34(2012 Nov)

The highly conserved NANOS2 protein: testis-specific expression and significance for the human male reproduction.
Kusz KM, Tomczyk L, Sajek M, Spik A, Latos-Bielenska A, Jedrzejczak P, Pawelczyk L, Jaruzelska J
Molecular human reproduction15(3)165-71(2009 Mar)

Expression of nitric oxide synthases in keratinocytes after UVB irradiation.
Chang HR, Tsao DA, Wang SR, Yu HS
Archives of dermatological research295(7)293-6(2003 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We conclude that heterogeneous population of the HEK293T cells might be easily shifted towards expression of the pluripotency markers by ectopic expression of the SON factors or by growth in serum depleted media.
Title: Pluripotency induction in HEK293T cells by concurrent expression of STELLA, OCT4 and NANOS2.

Data describe for the first time the expression pattern of the NANOS2 gene in human tissues and show that it is testis specific. We found that NANOS2 protein is present in prenatal germ cells and at later stages in spermatogenesis.
Title: The highly conserved NANOS2 protein: testis-specific expression and significance for the human male reproduction.

The increased NO production with increased expression of iNOS and ncNOS may contribute to the pathological and physiological features of UVB-induced erythema and skin inflammation.
Title: Expression of nitric oxide synthases in keratinocytes after UVB irradiation.

The following NANOS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NANOS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu30380	NM_001029861.3 Latest version!	Homo sapiens nanos C2HC-type zinc finger 2 (NANOS2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu30380	NM_001029861.2	Homo sapiens nanos C2HC-type zinc finger 2 (NANOS2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NANOS2 ( NM_001029861.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001025032.1 Homo sapiens nanos C2HC-type zinc finger 2 (NANOS2), mRNA.

Price & Availability↑

CloneID	OHu30380
Clone ID Related Accession (Same CDS sequence)	NM_001029861.2 , NM_001029861.3
Accession Version	NM_001029861.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-22
Organism	Homo sapiens(human)
Product	nanos homolog 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC042883.1, BC117484.1 and AC008623.4. On Aug 18, 2006 this sequence version replaced NM_001029861.1. ##Evidence-Data-START## Transcript is intronless :: BC042883.1 [ECO:0000345] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGCTGC CACCCTTCGA CATGTGGAAG GACTACTTCA ACCTGAGCCA GGTGGTGTGG 
GCGCTGATCG CAAGTCGGGG TCAAAGGCTG GAGACCCAAG AGATTGAGGA GCCAAGTCCC 
GGGCCTCCGC TGGGGCAGGA TCAGGGGCTG GGGGCGCCAG GGGCCAACGG GGGCCTGGGG 
ACCCTGTGCA ACTTCTGCAA GCACAACGGG GAGTCCCGCC ACGTCTACTC CTCACACCAG 
CTGAAGACAC CGGATGGCGT GGTGGTGTGT CCCATCCTGA GGCACTACGT GTGTCCCGTG 
TGCGGGGCCA CCGGTGACCA GGCCCATACG CTCAAGTACT GCCCGCTTAA CGGTGGCCAG 
CAGTCCCTCT ACCGCCGCAG CGGGCGCAAC TCGGCCGGAC GCAGGGTCAA GCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001025032.1
CDS	86..502
Translation	MQLPPFDMWKDYFNLSQVVWALIASRGQRLETQEIEEPSPGPPLGQDQGLGAPGANGGLGTLCNFCKHNGESRHVYSSHQLKTPDGVVVCPILRHYVCPVCGATGDQAHTLKYCPLNGGQQSLYRRSGRNSAGRRVKR

Target ORF information:

RefSeq Version	NM_001029861.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nanos C2HC-type zinc finger 2 (NANOS2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001029861.2

ORF Insert Sequence:

ATGCAGCTGC CACCCTTCGA CATGTGGAAG GACTACTTCA ACCTGAGCCA GGTGGTGTGG 
GCGCTGATCG CAAGTCGGGG TCAAAGGCTG GAGACCCAAG AGATTGAGGA GCCAAGTCCC 
GGGCCTCCGC TGGGGCAGGA TCAGGGGCTG GGGGCGCCAG GGGCCAACGG GGGCCTGGGG 
ACCCTGTGCA ACTTCTGCAA GCACAACGGG GAGTCCCGCC ACGTCTACTC CTCACACCAG 
CTGAAGACAC CGGATGGCGT GGTGGTGTGT CCCATCCTGA GGCACTACGT GTGTCCCGTG 
TGCGGGGCCA CCGGTGACCA GGCCCATACG CTCAAGTACT GCCCGCTTAA CGGTGGCCAG 
CAGTCCCTCT ACCGCCGCAG CGGGCGCAAC TCGGCCGGAC GCAGGGTCAA GCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NANOS2 ( NM_001029861.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001025032.1 Homo sapiens nanos C2HC-type zinc finger 2 (NANOS2), mRNA.

Price & Availability↑

CloneID	OHu30380
Clone ID Related Accession (Same CDS sequence)	NM_001029861.2 , NM_001029861.3
Accession Version	NM_001029861.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	nanos homolog 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC042883.1, BC117484.1 and AC008623.4. On May 17, 2019 this sequence version replaced NM_001029861.2. ##Evidence-Data-START## Transcript is intronless :: BC042883.1, BI463423.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000341294.4/ ENSP00000341021.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCAGCTGC CACCCTTCGA CATGTGGAAG GACTACTTCA ACCTGAGCCA GGTGGTGTGG 
GCGCTGATCG CAAGTCGGGG TCAAAGGCTG GAGACCCAAG AGATTGAGGA GCCAAGTCCC 
GGGCCTCCGC TGGGGCAGGA TCAGGGGCTG GGGGCGCCAG GGGCCAACGG GGGCCTGGGG 
ACCCTGTGCA ACTTCTGCAA GCACAACGGG GAGTCCCGCC ACGTCTACTC CTCACACCAG 
CTGAAGACAC CGGATGGCGT GGTGGTGTGT CCCATCCTGA GGCACTACGT GTGTCCCGTG 
TGCGGGGCCA CCGGTGACCA GGCCCATACG CTCAAGTACT GCCCGCTTAA CGGTGGCCAG 
CAGTCCCTCT ACCGCCGCAG CGGGCGCAAC TCGGCCGGAC GCAGGGTCAA GCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001025032.1
CDS	86..502
Translation	MQLPPFDMWKDYFNLSQVVWALIASRGQRLETQEIEEPSPGPPLGQDQGLGAPGANGGLGTLCNFCKHNGESRHVYSSHQLKTPDGVVVCPILRHYVCPVCGATGDQAHTLKYCPLNGGQQSLYRRSGRNSAGRRVKR

Target ORF information:

RefSeq Version	NM_001029861.3
Organism	Homo sapiens(human)
Definition	Homo sapiens nanos C2HC-type zinc finger 2 (NANOS2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001029861.3

ORF Insert Sequence:

ATGCAGCTGC CACCCTTCGA CATGTGGAAG GACTACTTCA ACCTGAGCCA GGTGGTGTGG 
GCGCTGATCG CAAGTCGGGG TCAAAGGCTG GAGACCCAAG AGATTGAGGA GCCAAGTCCC 
GGGCCTCCGC TGGGGCAGGA TCAGGGGCTG GGGGCGCCAG GGGCCAACGG GGGCCTGGGG 
ACCCTGTGCA ACTTCTGCAA GCACAACGGG GAGTCCCGCC ACGTCTACTC CTCACACCAG 
CTGAAGACAC CGGATGGCGT GGTGGTGTGT CCCATCCTGA GGCACTACGT GTGTCCCGTG 
TGCGGGGCCA CCGGTGACCA GGCCCATACG CTCAAGTACT GCCCGCTTAA CGGTGGCCAG 
CAGTCCCTCT ACCGCCGCAG CGGGCGCAAC TCGGCCGGAC GCAGGGTCAA GCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pluripotency induction in HEK293T cells by concurrent expression of STELLA, OCT4 and NANOS2.
Biochemical and biophysical research communications480(4)635-640(2016 Nov)
Farshchian M,Matin MM,Bahrami M,Geerts D,Dastpak M,Shahriyari M,Tajeran M,Mirahmadi M,Bahrami AR

Structural basis for the Nanos-mediated recruitment of the CCR4-NOT complex and translational repression.
Genes & development28(8)888-901(2014 Apr)
Bhandari D,Raisch T,Weichenrieder O,Jonas S,Izaurralde E

Analysis of meiosis regulators in human gonads: a sexually dimorphic spatio-temporal expression pattern suggests involvement of DMRT1 in meiotic entry.
Molecular human reproduction18(11)523-34(2012 Nov)
J?rgensen A,Nielsen JE,Blomberg Jensen M,Gr?m N,Rajpert-De Meyts E

The highly conserved NANOS2 protein: testis-specific expression and significance for the human male reproduction.
Molecular human reproduction15(3)165-71(2009 Mar)
Kusz KM,Tomczyk L,Sajek M,Spik A,Latos-Bielenska A,Jedrzejczak P,Pawelczyk L,Jaruzelska J

Expression of nitric oxide synthases in keratinocytes after UVB irradiation.
Archives of dermatological research295(7)293-6(2003 Dec)
Chang HR,Tsao DA,Wang SR,Yu HS