SERGEF cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SERGEF
Entrez Gene ID	26297
Full Name	secretion regulating guanine nucleotide exchange factor
Synonyms	DELGEF,Gnefr
General protein information	Preferred Names secretion regulating guanine nucleotide exchange factor Names secretion-regulating guanine nucleotide exchange factor deafness locus associated putative guanine nucleotide exchange factor guanine nucleotide exchange factor-related protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11p15.1
Disorder MIM:	606051

mRNA and Protein(s)

mRNA	Protein	Name
NM_012139.3	NP_036271.1	secretion-regulating guanine nucleotide exchange factor
NM_012139.4	NP_036271.1	secretion-regulating guanine nucleotide exchange factor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SERGEF	XP_003312986.1
Gallus gallus (chicken)	SERGEF	XP_004941476.1
Drosophila melanogaster (fruit fly)	CG7420	NP_608602.1
Saccharomyces cerevisiae (baker's yeast)	ATS1	NP_009382.2
Kluyveromyces lactis	KLLA0F15906g	XP_455796.1
Homo sapiens (human)	SERGEF	NP_036271.1
Bos taurus (cattle)	SERGEF	NP_001069489.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008272	XP_317198.4
Schizosaccharomyces pombe (fission yeast)	SPAC10F6.04	NP_593255.1
Mus musculus (house mouse)	Sergef	NP_038817.1
Danio rerio (zebrafish)	sergef	XP_694032.4
Magnaporthe oryzae (rice blast fungus)	MGG_05277	XP_003712836.1
Neurospora crassa	NCU00469	XP_956788.1
Canis lupus familiaris (dog)	SERGEF	XP_005633784.1
Rattus norvegicus (Norway rat)	Sergef	XP_344892.4
Xenopus tropicalis (tropical clawed frog)	MGC146459	NP_001072233.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

A Y2H-seq approach defines the human protein methyltransferase interactome.
Weimann M, Grossmann A, Woodsmith J, ?zkan Z, Birth P, Meierhofer D, Benlasfer N, Valovka T, Timmermann B, Wanker EE, Sauer S, Stelzl U
Nature methods10(4)339-42(2013 Apr)

Characterisation of an evolutionary conserved protein interacting with the putative guanine nucleotide exchange factor DelGEF and modulating secretion.
Sj?linder M, Uhlmann J, Ponstingl H
Experimental cell research294(1)68-76(2004 Mar)

DelGEF, a homologue of the Ran guanine nucleotide exchange factor RanGEF, binds to the exocyst component Sec5 and modulates secretion.
Sj?linder M, Uhlmann J, Ponstingl H
FEBS letters532(1-2)211-5(2002 Dec)

DelGEF, an RCC1-related protein encoded by a gene on chromosome 11p14 critical for two forms of hereditary deafness.
Uhlmann J, Wiemann S, Ponstingl H
FEBS letters460(1)153-60(1999 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DelGEF binds to the human homologue of Sec5 and modulates secretion.
Title: DelGEF, a homologue of the Ran guanine nucleotide exchange factor RanGEF, binds to the exocyst component Sec5 and modulates secretion.

The following SERGEF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SERGEF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu06075	NM_012139.4 Latest version!	Homo sapiens secretion regulating guanine nucleotide exchange factor (SERGEF), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
View Old Accession Versions >>
OHu06075	NM_012139.3	Homo sapiens secretion regulating guanine nucleotide exchange factor (SERGEF), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SERGEF ( NM_012139.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_036271.1 Homo sapiens secretion regulating guanine nucleotide exchange factor (SERGEF), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu06075
Clone ID Related Accession (Same CDS sequence)	NM_012139.3 , NM_012139.4
Accession Version	NM_012139.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	secretion-regulating guanine nucleotide exchange factor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK292286.1 and BC065375.1. On Aug 2, 2013 this sequence version replaced NM_012139.2. Transcript Variant: This variant (1) represents the protein-coding transcript. ##Evidence-Data-START## Transcript exon combination :: BC065375.1, SRR1163658.295660.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCGCG AGCCCAGCGC CTCGGAGGCC GCCCCCGCGG CGGCCGCGCT CTTCGCCTGG 
GGTGCAAATA GCTATGGGCA ACTTGGCCTC GGCCATAAGG AAGATGTGCT GTTGCCCCAG 
CAACTGAATG ACTTCTGTAA ACCCAGGAGT GTCAGGAGGA TCACAGGAGG AGGGGGCCAC 
TCTGCAGTTG TCACAGATGG AGGAGACCTC TTTGTTTGTG GCCTGAACAA AGATGGGCAA 
CTGGGGCTTG GTCACACAGA GGATATCCCA TATTTTACCC CCTGCAAATC CCTCTTTGGC 
TGTCCCATCC AACAGGTGGC CTGTGGCTGG GATTTTACGA TTATGCTCAC AGAAAATGGT 
CAAGTTCTAT CATGTGGATC CAACTCCTTT GGCCAGTTAG GAGTTCCTCA TGGACCTCGA 
AGATGTGTGG TTCCCCAGGC CATTGAGCTC CATAAAGAGA AGGTTGTTTG TATTGCTGCT 
GGACTGAGGC ATGCAGTAGC TGCTACAGCG AGTGGCATCG TGTTCCAGTG GGGGACTGGT 
TTGGCATCAT GTGGACGACG GTTGTGCCCT GGGCAGACTC TTCCATTGTT TTTCACAGCA 
AAGGAACCAA GCAGAGTGAC AGGTCTAGAG AATTCTAAAG CAATGTGTGT TCTTGCTGGC 
TCAGACCACT CAGCTTCATT AACAGATGCA GGAGAGGTGT ATGTTTGGGG AAGCAACAAG 
CATGGGCAAC TGGCTAATGA GGCTGCTTTC CTTCCTGTGC CCCAGAAAAT AGAAGCACAT 
TGTTTCCAGA ATGAAAAGGT CACTGCCATC TGGAGTGGAT GGACACACCT GGTTGCTCAG 
ACAGAAACTG GCAAGATGTT TACCTGGGGC CGAGCAGACT ATGGTCAGCT AGGGAGGAAG 
TTGGAGACTT ATGAAGGCTG GAAACTAGAA AAGCAAGATT CATTTCTCCC CTGTTCAAGA 
CCACCGAACA GCATGCCTTC GTCTCCGCAT TGCTTAACTG GAGCAACTGA GGTCTCTTGT 
GGCTCAGAGC ATAATTTGGC AATAATTGGT GGAGTGTGTT ACTCTTGGGG CTGGAATGAG 
CATGGCATGT GCGGAGATGG CACTGAAGCC AACGTCTGGG CCCCAAAGCC GGTGCAGGCT 
CTGCTGTCAT CGTCAGGACT CCTTGTGGGC TGTGGGGCTG GCCACTCCTT GGCCCTCTGC 
CAGCTGCCAG CTCACCCTGC ATTGGTCCAG GACCCCAAGG TCACCTACCT TTCCCCAGAT 
GCCATCGAGG ACACTGAATC TCAGAAAGCC ATGGACAAAG AGAGAAACTG GAAGGAAAGA 
CAATCAGAAA CTTCAACCCA AAGCCAATCT GACTGGTCCA GAAATGGGGG ACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036271.1
CDS	153..1529
Translation	MEREPSASEAAPAAAALFAWGANSYGQLGLGHKEDVLLPQQLNDFCKPRSVRRITGGGGHSAVVTDGGDLFVCGLNKDGQLGLGHTEDIPYFTPCKSLFGCPIQQVACGWDFTIMLTENGQVLSCGSNSFGQLGVPHGPRRCVVPQAIELHKEKVVCIAAGLRHAVAATASGIVFQWGTGLASCGRRLCPGQTLPLFFTAKEPSRVTGLENSKAMCVLAGSDHSASLTDAGEVYVWGSNKHGQLANEAAFLPVPQKIEAHCFQNEKVTAIWSGWTHLVAQTETGKMFTWGRADYGQLGRKLETYEGWKLEKQDSFLPCSRPPNSMPSSPHCLTGATEVSCGSEHNLAIIGGVCYSWGWNEHGMCGDGTEANVWAPKPVQALLSSSGLLVGCGAGHSLALCQLPAHPALVQDPKVTYLSPDAIEDTESQKAMDKERNWKERQSETSTQSQSDWSRNGGL

Target ORF information:

RefSeq Version	NM_012139.3
Organism	Homo sapiens(human)
Definition	Homo sapiens secretion regulating guanine nucleotide exchange factor (SERGEF), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012139.3

ORF Insert Sequence:

ATGGAGCGCG AGCCCAGCGC CTCGGAGGCC GCCCCCGCGG CGGCCGCGCT CTTCGCCTGG 
GGTGCAAATA GCTATGGGCA ACTTGGCCTC GGCCATAAGG AAGATGTGCT GTTGCCCCAG 
CAACTGAATG ACTTCTGTAA ACCCAGGAGT GTCAGGAGGA TCACAGGAGG AGGGGGCCAC 
TCTGCAGTTG TCACAGATGG AGGAGACCTC TTTGTTTGTG GCCTGAACAA AGATGGGCAA 
CTGGGGCTTG GTCACACAGA GGATATCCCA TATTTTACCC CCTGCAAATC CCTCTTTGGC 
TGTCCCATCC AACAGGTGGC CTGTGGCTGG GATTTTACGA TTATGCTCAC AGAAAATGGT 
CAAGTTCTAT CATGTGGATC CAACTCCTTT GGCCAGTTAG GAGTTCCTCA TGGACCTCGA 
AGATGTGTGG TTCCCCAGGC CATTGAGCTC CATAAAGAGA AGGTTGTTTG TATTGCTGCT 
GGACTGAGGC ATGCAGTAGC TGCTACAGCG AGTGGCATCG TGTTCCAGTG GGGGACTGGT 
TTGGCATCAT GTGGACGACG GTTGTGCCCT GGGCAGACTC TTCCATTGTT TTTCACAGCA 
AAGGAACCAA GCAGAGTGAC AGGTCTAGAG AATTCTAAAG CAATGTGTGT TCTTGCTGGC 
TCAGACCACT CAGCTTCATT AACAGATGCA GGAGAGGTGT ATGTTTGGGG AAGCAACAAG 
CATGGGCAAC TGGCTAATGA GGCTGCTTTC CTTCCTGTGC CCCAGAAAAT AGAAGCACAT 
TGTTTCCAGA ATGAAAAGGT CACTGCCATC TGGAGTGGAT GGACACACCT GGTTGCTCAG 
ACAGAAACTG GCAAGATGTT TACCTGGGGC CGAGCAGACT ATGGTCAGCT AGGGAGGAAG 
TTGGAGACTT ATGAAGGCTG GAAACTAGAA AAGCAAGATT CATTTCTCCC CTGTTCAAGA 
CCACCGAACA GCATGCCTTC GTCTCCGCAT TGCTTAACTG GAGCAACTGA GGTCTCTTGT 
GGCTCAGAGC ATAATTTGGC AATAATTGGT GGAGTGTGTT ACTCTTGGGG CTGGAATGAG 
CATGGCATGT GCGGAGATGG CACTGAAGCC AACGTCTGGG CCCCAAAGCC GGTGCAGGCT 
CTGCTGTCAT CGTCAGGACT CCTTGTGGGC TGTGGGGCTG GCCACTCCTT GGCCCTCTGC 
CAGCTGCCAG CTCACCCTGC ATTGGTCCAG GACCCCAAGG TCACCTACCT TTCCCCAGAT 
GCCATCGAGG ACACTGAATC TCAGAAAGCC ATGGACAAAG AGAGAAACTG GAAGGAAAGA 
CAATCAGAAA CTTCAACCCA AAGCCAATCT GACTGGTCCA GAAATGGGGG ACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SERGEF ( NM_012139.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_036271.1 Homo sapiens secretion regulating guanine nucleotide exchange factor (SERGEF), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu06075
Clone ID Related Accession (Same CDS sequence)	NM_012139.3 , NM_012139.4
Accession Version	NM_012139.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	secretion-regulating guanine nucleotide exchange factor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK292286.1 and BC065375.1. On Nov 23, 2018 this sequence version replaced NM_012139.3. Transcript Variant: This variant (1) represents the protein-coding transcript. ##Evidence-Data-START## Transcript exon combination :: BC065375.1, AK292286.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000265965.10/ ENSP00000265965.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGCGCG AGCCCAGCGC CTCGGAGGCC GCCCCCGCGG CGGCCGCGCT CTTCGCCTGG 
GGTGCAAATA GCTATGGGCA ACTTGGCCTC GGCCATAAGG AAGATGTGCT GTTGCCCCAG 
CAACTGAATG ACTTCTGTAA ACCCAGGAGT GTCAGGAGGA TCACAGGAGG AGGGGGCCAC 
TCTGCAGTTG TCACAGATGG AGGAGACCTC TTTGTTTGTG GCCTGAACAA AGATGGGCAA 
CTGGGGCTTG GTCACACAGA GGATATCCCA TATTTTACCC CCTGCAAATC CCTCTTTGGC 
TGTCCCATCC AACAGGTGGC CTGTGGCTGG GATTTTACGA TTATGCTCAC AGAAAATGGT 
CAAGTTCTAT CATGTGGATC CAACTCCTTT GGCCAGTTAG GAGTTCCTCA TGGACCTCGA 
AGATGTGTGG TTCCCCAGGC CATTGAGCTC CATAAAGAGA AGGTTGTTTG TATTGCTGCT 
GGACTGAGGC ATGCAGTAGC TGCTACAGCG AGTGGCATCG TGTTCCAGTG GGGGACTGGT 
TTGGCATCAT GTGGACGACG GTTGTGCCCT GGGCAGACTC TTCCATTGTT TTTCACAGCA 
AAGGAACCAA GCAGAGTGAC AGGTCTAGAG AATTCTAAAG CAATGTGTGT TCTTGCTGGC 
TCAGACCACT CAGCTTCATT AACAGATGCA GGAGAGGTGT ATGTTTGGGG AAGCAACAAG 
CATGGGCAAC TGGCTAATGA GGCTGCTTTC CTTCCTGTGC CCCAGAAAAT AGAAGCACAT 
TGTTTCCAGA ATGAAAAGGT CACTGCCATC TGGAGTGGAT GGACACACCT GGTTGCTCAG 
ACAGAAACTG GCAAGATGTT TACCTGGGGC CGAGCAGACT ATGGTCAGCT AGGGAGGAAG 
TTGGAGACTT ATGAAGGCTG GAAACTAGAA AAGCAAGATT CATTTCTCCC CTGTTCAAGA 
CCACCGAACA GCATGCCTTC GTCTCCGCAT TGCTTAACTG GAGCAACTGA GGTCTCTTGT 
GGCTCAGAGC ATAATTTGGC AATAATTGGT GGAGTGTGTT ACTCTTGGGG CTGGAATGAG 
CATGGCATGT GCGGAGATGG CACTGAAGCC AACGTCTGGG CCCCAAAGCC GGTGCAGGCT 
CTGCTGTCAT CGTCAGGACT CCTTGTGGGC TGTGGGGCTG GCCACTCCTT GGCCCTCTGC 
CAGCTGCCAG CTCACCCTGC ATTGGTCCAG GACCCCAAGG TCACCTACCT TTCCCCAGAT 
GCCATCGAGG ACACTGAATC TCAGAAAGCC ATGGACAAAG AGAGAAACTG GAAGGAAAGA 
CAATCAGAAA CTTCAACCCA AAGCCAATCT GACTGGTCCA GAAATGGGGG ACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036271.1
CDS	38..1414
Translation	MEREPSASEAAPAAAALFAWGANSYGQLGLGHKEDVLLPQQLNDFCKPRSVRRITGGGGHSAVVTDGGDLFVCGLNKDGQLGLGHTEDIPYFTPCKSLFGCPIQQVACGWDFTIMLTENGQVLSCGSNSFGQLGVPHGPRRCVVPQAIELHKEKVVCIAAGLRHAVAATASGIVFQWGTGLASCGRRLCPGQTLPLFFTAKEPSRVTGLENSKAMCVLAGSDHSASLTDAGEVYVWGSNKHGQLANEAAFLPVPQKIEAHCFQNEKVTAIWSGWTHLVAQTETGKMFTWGRADYGQLGRKLETYEGWKLEKQDSFLPCSRPPNSMPSSPHCLTGATEVSCGSEHNLAIIGGVCYSWGWNEHGMCGDGTEANVWAPKPVQALLSSSGLLVGCGAGHSLALCQLPAHPALVQDPKVTYLSPDAIEDTESQKAMDKERNWKERQSETSTQSQSDWSRNGGL

Target ORF information:

RefSeq Version	NM_012139.4
Organism	Homo sapiens(human)
Definition	Homo sapiens secretion regulating guanine nucleotide exchange factor (SERGEF), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012139.4

ORF Insert Sequence:

ATGGAGCGCG AGCCCAGCGC CTCGGAGGCC GCCCCCGCGG CGGCCGCGCT CTTCGCCTGG 
GGTGCAAATA GCTATGGGCA ACTTGGCCTC GGCCATAAGG AAGATGTGCT GTTGCCCCAG 
CAACTGAATG ACTTCTGTAA ACCCAGGAGT GTCAGGAGGA TCACAGGAGG AGGGGGCCAC 
TCTGCAGTTG TCACAGATGG AGGAGACCTC TTTGTTTGTG GCCTGAACAA AGATGGGCAA 
CTGGGGCTTG GTCACACAGA GGATATCCCA TATTTTACCC CCTGCAAATC CCTCTTTGGC 
TGTCCCATCC AACAGGTGGC CTGTGGCTGG GATTTTACGA TTATGCTCAC AGAAAATGGT 
CAAGTTCTAT CATGTGGATC CAACTCCTTT GGCCAGTTAG GAGTTCCTCA TGGACCTCGA 
AGATGTGTGG TTCCCCAGGC CATTGAGCTC CATAAAGAGA AGGTTGTTTG TATTGCTGCT 
GGACTGAGGC ATGCAGTAGC TGCTACAGCG AGTGGCATCG TGTTCCAGTG GGGGACTGGT 
TTGGCATCAT GTGGACGACG GTTGTGCCCT GGGCAGACTC TTCCATTGTT TTTCACAGCA 
AAGGAACCAA GCAGAGTGAC AGGTCTAGAG AATTCTAAAG CAATGTGTGT TCTTGCTGGC 
TCAGACCACT CAGCTTCATT AACAGATGCA GGAGAGGTGT ATGTTTGGGG AAGCAACAAG 
CATGGGCAAC TGGCTAATGA GGCTGCTTTC CTTCCTGTGC CCCAGAAAAT AGAAGCACAT 
TGTTTCCAGA ATGAAAAGGT CACTGCCATC TGGAGTGGAT GGACACACCT GGTTGCTCAG 
ACAGAAACTG GCAAGATGTT TACCTGGGGC CGAGCAGACT ATGGTCAGCT AGGGAGGAAG 
TTGGAGACTT ATGAAGGCTG GAAACTAGAA AAGCAAGATT CATTTCTCCC CTGTTCAAGA 
CCACCGAACA GCATGCCTTC GTCTCCGCAT TGCTTAACTG GAGCAACTGA GGTCTCTTGT 
GGCTCAGAGC ATAATTTGGC AATAATTGGT GGAGTGTGTT ACTCTTGGGG CTGGAATGAG 
CATGGCATGT GCGGAGATGG CACTGAAGCC AACGTCTGGG CCCCAAAGCC GGTGCAGGCT 
CTGCTGTCAT CGTCAGGACT CCTTGTGGGC TGTGGGGCTG GCCACTCCTT GGCCCTCTGC 
CAGCTGCCAG CTCACCCTGC ATTGGTCCAG GACCCCAAGG TCACCTACCT TTCCCCAGAT 
GCCATCGAGG ACACTGAATC TCAGAAAGCC ATGGACAAAG AGAGAAACTG GAAGGAAAGA 
CAATCAGAAA CTTCAACCCA AAGCCAATCT GACTGGTCCA GAAATGGGGG ACTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

A Y2H-seq approach defines the human protein methyltransferase interactome.
Nature methods10(4)339-42(2013 Apr)
Weimann M,Grossmann A,Woodsmith J,?zkan Z,Birth P,Meierhofer D,Benlasfer N,Valovka T,Timmermann B,Wanker EE,Sauer S,Stelzl U

Characterisation of an evolutionary conserved protein interacting with the putative guanine nucleotide exchange factor DelGEF and modulating secretion.
Experimental cell research294(1)68-76(2004 Mar)
Sj?linder M,Uhlmann J,Ponstingl H

DelGEF, a homologue of the Ran guanine nucleotide exchange factor RanGEF, binds to the exocyst component Sec5 and modulates secretion.
FEBS letters532(1-2)211-5(2002 Dec)
Sj?linder M,Uhlmann J,Ponstingl H

DelGEF, an RCC1-related protein encoded by a gene on chromosome 11p14 critical for two forms of hereditary deafness.
FEBS letters460(1)153-60(1999 Oct)
Uhlmann J,Wiemann S,Ponstingl H