TIPRL cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TIPRL
Entrez Gene ID	261726
Full Name	TOR signaling pathway regulator
Synonyms	TIP,TIP41,TIPRL1
General protein information	Preferred Names TOR signaling pathway regulator Names TIP41-like protein TIP41, TOR signaling pathway regulator-like putative MAPK-activating protein PM10 type 2A-interacting protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q24.2
Summary	TIPRL is an inhibitory regulator of protein phosphatase-2A (PP2A) (see PPP2CA; MIM 176915), PP4 (see PPP4C; MIM 602035), and PP6 (see PPP6C; MIM 612725) (McConnell et al., 2007 [PubMed 17384681]).[supplied by OMIM, Nov 2010].
Disorder MIM:	611807

mRNA and Protein(s)

mRNA	Protein	Name
NM_001031800.2	NP_001026970.1	TIP41-like protein isoform 2
NM_152902.5	NP_690866.1	TIP41-like protein isoform 1
NM_152902.4	NP_690866.1	TIP41-like protein isoform 1
NM_001031800.3	NP_001026970.1	TIP41-like protein isoform 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	TIPRL	XP_537207.3
Rattus norvegicus (Norway rat)	Tiprl	NP_001103137.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002657	XP_563488.3
Oryza sativa (rice)	Os03g0760600	NP_001051346.1
Gallus gallus (chicken)	TIPRL	XP_416593.1
Eremothecium gossypii	AGOS_ACR174C	NP_983576.1
Neurospora crassa	NCU00109	XP_957233.2
	TIPRL	XP_001091944.2
Danio rerio (zebrafish)	tiprl	NP_956957.1
Drosophila melanogaster (fruit fly)	CG9578	NP_608377.1
Caenorhabditis elegans (roundworm)	ZK688.9	NP_498713.3
Kluyveromyces lactis	KLLA0E06139g	XP_454223.1
Homo sapiens (human)	TIPRL	NP_690866.1
Pan troglodytes (chimpanzee)	TIPRL	XP_513978.3
Bos taurus (cattle)	TIPRL	NP_001073083.1
Mus musculus (house mouse)	Tiprl	NP_663488.1
Saccharomyces cerevisiae (baker's yeast)	TIP41	NP_015365.1
Magnaporthe oryzae (rice blast fungus)	MGG_00478	XP_003718584.1
Arabidopsis thaliana (thale cress)	AT4G34270	NP_195153.2
Xenopus tropicalis (tropical clawed frog)	tiprl	NP_001015736.1

Gene Ontology
Bibliography

Related articles in PubMed

TIPRL Inhibits Protein Phosphatase 4 Activity and Promotes H2AX Phosphorylation in the DNA Damage Response.
Rosales KR, Reid MA, Yang Y, Tran TQ, Wang WI, Lowman X, Pan M, Kong M
PloS one10(12)e0145938(2015)

Protein interaction network of the mammalian Hippo pathway reveals mechanisms of kinase-phosphatase interactions.
Couzens AL, Knight JD, Kean MJ, Teo G, Weiss A, Dunham WH, Lin ZY, Bagshaw RD, Sicheri F, Pawson T, Wrana JL, Choi H, Gingras AC
Science signaling6(302)rs15(2013 Nov)

A positive role of mammalian Tip41-like protein, TIPRL, in the amino-acid dependent mTORC1-signaling pathway through interaction with PP2A.
Nakashima A, Tanimura-Ito K, Oshiro N, Eguchi S, Miyamoto T, Momonami A, Kamada S, Yonezawa K, Kikkawa U
FEBS letters587(18)2924-9(2013 Sep)

Inhibition of MKK7-JNK by the TOR signaling pathway regulator-like protein contributes to resistance of HCC cells to TRAIL-induced apoptosis.
Song IS, Jun SY, Na HJ, Kim HT, Jung SY, Ha GH, Park YH, Long LZ, Yu DY, Kim JM, Kim JH, Ko JH, Kim CH, Kim NS
Gastroenterology143(5)1341-51(2012 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

overexpression of TIPRL promotes phosphorylation of H2AX, and increases gamma-H2AX positive foci in response to DNA damage, whereas knockdown of TIPRL inhibits gamma-H2AX phosphorylation
Title: TIPRL Inhibits Protein Phosphatase 4 Activity and Promotes H2AX Phosphorylation in the DNA Damage Response.

Unlike yeast TIP41, TIPRL has a positive effect on mTORC1 signaling through the association with PP2Ac.
Title: A positive role of mammalian Tip41-like protein, TIPRL, in the amino-acid dependent mTORC1-signaling pathway through interaction with PP2A.

Data indicate that PP2A holoenzyme biogenesis and activity are controlled by five PP2A modulators, consisting of alpha4, PTPA, LCMT1, PME-1 and TIPRL1, which serve to prevent promiscuous phosphatase activity until the holoenzyme is completely assembled.
Title: The biogenesis of active protein phosphatase 2A holoenzymes: a tightly regulated process creating phosphatase specificity.

TIPRL is highly up-regulated in human HCC samples and cell lines, compared with noncancerous liver tissues.
Title: Inhibition of MKK7-JNK by the TOR signaling pathway regulator-like protein contributes to resistance of HCC cells to TRAIL-induced apoptosis.

Observational study of gene-disease association. (HuGE Navigator)
Title: Follow-up of a major linkage peak on chromosome 1 reveals suggestive QTLs associated with essential hypertension: GenNet study.

The following TIPRL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TIPRL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu09545	NM_001031800.3 Latest version!	Homo sapiens TOR signaling pathway regulator (TIPRL), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu09534	NM_152902.5 Latest version!	Homo sapiens TOR signaling pathway regulator (TIPRL), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
View Old Accession Versions >>
OHu09534	NM_152902.4	Homo sapiens TOR signaling pathway regulator (TIPRL), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu09545	NM_001031800.2	Homo sapiens TOR signaling pathway regulator (TIPRL), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TIPRL ( NM_001031800.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001026970.1 Homo sapiens TOR signaling pathway regulator (TIPRL), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu09545
Clone ID Related Accession (Same CDS sequence)	NM_001031800.2 , NM_001031800.3
Accession Version	NM_001031800.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	TIP41-like protein isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK313520.1 and BC009506.2. On Dec 11, 2013 this sequence version replaced NM_001031800.1. Transcript Variant: This variant (2) lacks several exons, and uses an alternate 3'-terminal exon, compared to variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (2) has a shorter and distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC009506.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147975, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGATCC ACGGCTTCCA GAGCAGCCAC CGGGATTTCT GCTTCGGGCC CTGGAAGCTG 
ACGGCGTCCA AGACCCACAT CATGAAGTCG GCGGATGTGG AGAAATTAGC CGATGAATTA 
CATATGCCAT CTCTCCCTGA AATGATGTTT GGAGACAACG TTTTAAGAAT CCAGCATGGG 
TCTGGCTTTG GAATTGAGTT CAATGCTACA GATGCGTTAA GATGTGTAAA CAACTACCAA 
GGAATGCTTA AAGTGGCCTG TGCTGAAGAG TGGCAAGAAA GCAGGACGGA GGGTGAACAC 
TCCAAAGAGG TTATTAAACC ATATGATTGG ACCTATACAA CAGATTATAA GGGAACCTTA 
CTTGGAGAAT CTCTTAAGTT AAAGGTTGTA CCTACAACAG ATCATATAGA TACAGAAAAA 
TTGAAAGCCA GAGAACAGAT TAAGTTTTTT GAAGAAGTTC TCCTTTTTGA GGATGAACTT 
CATGATCATG GAGTTTCAAG CCTGAGTGTG AAGATTCCTG GTGGCGGGCA CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001026970.1
CDS	234..770
Translation	MMIHGFQSSHRDFCFGPWKLTASKTHIMKSADVEKLADELHMPSLPEMMFGDNVLRIQHGSGFGIEFNATDALRCVNNYQGMLKVACAEEWQESRTEGEHSKEVIKPYDWTYTTDYKGTLLGESLKLKVVPTTDHIDTEKLKAREQIKFFEEVLLFEDELHDHGVSSLSVKIPGGGHL

Target ORF information:

RefSeq Version	NM_001031800.2
Organism	Homo sapiens(human)
Definition	Homo sapiens TOR signaling pathway regulator (TIPRL), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001031800.2

ORF Insert Sequence:

ATGATGATCC ACGGCTTCCA GAGCAGCCAC CGGGATTTCT GCTTCGGGCC CTGGAAGCTG 
ACGGCGTCCA AGACCCACAT CATGAAGTCG GCGGATGTGG AGAAATTAGC CGATGAATTA 
CATATGCCAT CTCTCCCTGA AATGATGTTT GGAGACAACG TTTTAAGAAT CCAGCATGGG 
TCTGGCTTTG GAATTGAGTT CAATGCTACA GATGCGTTAA GATGTGTAAA CAACTACCAA 
GGAATGCTTA AAGTGGCCTG TGCTGAAGAG TGGCAAGAAA GCAGGACGGA GGGTGAACAC 
TCCAAAGAGG TTATTAAACC ATATGATTGG ACCTATACAA CAGATTATAA GGGAACCTTA 
CTTGGAGAAT CTCTTAAGTT AAAGGTTGTA CCTACAACAG ATCATATAGA TACAGAAAAA 
TTGAAAGCCA GAGAACAGAT TAAGTTTTTT GAAGAAGTTC TCCTTTTTGA GGATGAACTT 
CATGATCATG GAGTTTCAAG CCTGAGTGTG AAGATTCCTG GTGGCGGGCA CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIPRL ( NM_001031800.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001026970.1 Homo sapiens TOR signaling pathway regulator (TIPRL), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu09545
Clone ID Related Accession (Same CDS sequence)	NM_001031800.2 , NM_001031800.3
Accession Version	NM_001031800.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	TIP41-like protein isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK313520.1 and BC009506.2. On May 31, 2019 this sequence version replaced NM_001031800.2. Transcript Variant: This variant (2) lacks several exons, and uses an alternate 3'-terminal exon, compared to variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (2) has a shorter and distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC009506.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147975, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGATCC ACGGCTTCCA GAGCAGCCAC CGGGATTTCT GCTTCGGGCC CTGGAAGCTG 
ACGGCGTCCA AGACCCACAT CATGAAGTCG GCGGATGTGG AGAAATTAGC CGATGAATTA 
CATATGCCAT CTCTCCCTGA AATGATGTTT GGAGACAACG TTTTAAGAAT CCAGCATGGG 
TCTGGCTTTG GAATTGAGTT CAATGCTACA GATGCGTTAA GATGTGTAAA CAACTACCAA 
GGAATGCTTA AAGTGGCCTG TGCTGAAGAG TGGCAAGAAA GCAGGACGGA GGGTGAACAC 
TCCAAAGAGG TTATTAAACC ATATGATTGG ACCTATACAA CAGATTATAA GGGAACCTTA 
CTTGGAGAAT CTCTTAAGTT AAAGGTTGTA CCTACAACAG ATCATATAGA TACAGAAAAA 
TTGAAAGCCA GAGAACAGAT TAAGTTTTTT GAAGAAGTTC TCCTTTTTGA GGATGAACTT 
CATGATCATG GAGTTTCAAG CCTGAGTGTG AAGATTCCTG GTGGCGGGCA CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001026970.1
CDS	117..653
Translation	MMIHGFQSSHRDFCFGPWKLTASKTHIMKSADVEKLADELHMPSLPEMMFGDNVLRIQHGSGFGIEFNATDALRCVNNYQGMLKVACAEEWQESRTEGEHSKEVIKPYDWTYTTDYKGTLLGESLKLKVVPTTDHIDTEKLKAREQIKFFEEVLLFEDELHDHGVSSLSVKIPGGGHL

Target ORF information:

RefSeq Version	NM_001031800.3
Organism	Homo sapiens(human)
Definition	Homo sapiens TOR signaling pathway regulator (TIPRL), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001031800.3

ORF Insert Sequence:

ATGATGATCC ACGGCTTCCA GAGCAGCCAC CGGGATTTCT GCTTCGGGCC CTGGAAGCTG 
ACGGCGTCCA AGACCCACAT CATGAAGTCG GCGGATGTGG AGAAATTAGC CGATGAATTA 
CATATGCCAT CTCTCCCTGA AATGATGTTT GGAGACAACG TTTTAAGAAT CCAGCATGGG 
TCTGGCTTTG GAATTGAGTT CAATGCTACA GATGCGTTAA GATGTGTAAA CAACTACCAA 
GGAATGCTTA AAGTGGCCTG TGCTGAAGAG TGGCAAGAAA GCAGGACGGA GGGTGAACAC 
TCCAAAGAGG TTATTAAACC ATATGATTGG ACCTATACAA CAGATTATAA GGGAACCTTA 
CTTGGAGAAT CTCTTAAGTT AAAGGTTGTA CCTACAACAG ATCATATAGA TACAGAAAAA 
TTGAAAGCCA GAGAACAGAT TAAGTTTTTT GAAGAAGTTC TCCTTTTTGA GGATGAACTT 
CATGATCATG GAGTTTCAAG CCTGAGTGTG AAGATTCCTG GTGGCGGGCA CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIPRL ( NM_152902.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_690866.1 Homo sapiens TOR signaling pathway regulator (TIPRL), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu09534
Clone ID Related Accession (Same CDS sequence)	NM_152902.5 , NM_152902.4
Accession Version	NM_152902.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Homo sapiens(human)
Product	TIP41-like protein isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK313520.1 and AL021397.1. On Nov 22, 2018 this sequence version replaced NM_152902.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.980507.1, SRR3476690.906064.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000367833.7/ ENSP00000356807.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGATGATCC ACGGCTTCCA GAGCAGCCAC CGGGATTTCT GCTTCGGGCC CTGGAAGCTG 
ACGGCGTCCA AGACCCACAT CATGAAGTCG GCGGATGTGG AGAAATTAGC CGATGAATTA 
CATATGCCAT CTCTCCCTGA AATGATGTTT GGAGACAACG TTTTAAGAAT CCAGCATGGG 
TCTGGCTTTG GAATTGAGTT CAATGCTACA GATGCGTTAA GATGTGTAAA CAACTACCAA 
GGAATGCTTA AAGTGGCCTG TGCTGAAGAG TGGCAAGAAA GCAGGACGGA GGGTGAACAC 
TCCAAAGAGG TTATTAAACC ATATGATTGG ACCTATACAA CAGATTATAA GGGAACCTTA 
CTTGGAGAAT CTCTTAAGTT AAAGGTTGTA CCTACAACAG ATCATATAGA TACAGAAAAA 
TTGAAAGCCA GAGAACAGAT TAAGTTTTTT GAAGAAGTTC TCCTTTTTGA GGATGAACTT 
CATGATCATG GAGTTTCAAG CCTGAGTGTG AAGATTAGAG TAATGCCTTC TAGCTTTTTC 
CTGCTGTTGC GGTTTTTCTT GAGAATTGAT GGGGTGCTTA TCAGAATGAA TGACACGAGA 
CTTTACCATG AGGCTGACAA GACCTACATG TTACGAGAAT ATACGTCACG AGAAAGCAAA 
ATTTCTAGTT TGATGCATGT TCCACCTTCC CTCTTCACGG AACCTAATGA AATATCCCAG 
TATTTACCAA TAAAGGAAGC AGTTTGTGAG AAGCTAATAT TTCCAGAAAG AATTGATCCT 
AACCCAGCAG ACTCACAAAA AAGTACACAA GTGGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_690866.1
CDS	117..935
Translation	MMIHGFQSSHRDFCFGPWKLTASKTHIMKSADVEKLADELHMPSLPEMMFGDNVLRIQHGSGFGIEFNATDALRCVNNYQGMLKVACAEEWQESRTEGEHSKEVIKPYDWTYTTDYKGTLLGESLKLKVVPTTDHIDTEKLKAREQIKFFEEVLLFEDELHDHGVSSLSVKIRVMPSSFFLLLRFFLRIDGVLIRMNDTRLYHEADKTYMLREYTSRESKISSLMHVPPSLFTEPNEISQYLPIKEAVCEKLIFPERIDPNPADSQKSTQVE

Target ORF information:

RefSeq Version	NM_152902.5
Organism	Homo sapiens(human)
Definition	Homo sapiens TOR signaling pathway regulator (TIPRL), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152902.5

ORF Insert Sequence:

ATGATGATCC ACGGCTTCCA GAGCAGCCAC CGGGATTTCT GCTTCGGGCC CTGGAAGCTG 
ACGGCGTCCA AGACCCACAT CATGAAGTCG GCGGATGTGG AGAAATTAGC CGATGAATTA 
CATATGCCAT CTCTCCCTGA AATGATGTTT GGAGACAACG TTTTAAGAAT CCAGCATGGG 
TCTGGCTTTG GAATTGAGTT CAATGCTACA GATGCGTTAA GATGTGTAAA CAACTACCAA 
GGAATGCTTA AAGTGGCCTG TGCTGAAGAG TGGCAAGAAA GCAGGACGGA GGGTGAACAC 
TCCAAAGAGG TTATTAAACC ATATGATTGG ACCTATACAA CAGATTATAA GGGAACCTTA 
CTTGGAGAAT CTCTTAAGTT AAAGGTTGTA CCTACAACAG ATCATATAGA TACAGAAAAA 
TTGAAAGCCA GAGAACAGAT TAAGTTTTTT GAAGAAGTTC TCCTTTTTGA GGATGAACTT 
CATGATCATG GAGTTTCAAG CCTGAGTGTG AAGATTAGAG TAATGCCTTC TAGCTTTTTC 
CTGCTGTTGC GGTTTTTCTT GAGAATTGAT GGGGTGCTTA TCAGAATGAA TGACACGAGA 
CTTTACCATG AGGCTGACAA GACCTACATG TTACGAGAAT ATACGTCACG AGAAAGCAAA 
ATTTCTAGTT TGATGCATGT TCCACCTTCC CTCTTCACGG AACCTAATGA AATATCCCAG 
TATTTACCAA TAAAGGAAGC AGTTTGTGAG AAGCTAATAT TTCCAGAAAG AATTGATCCT 
AACCCAGCAG ACTCACAAAA AAGTACACAA GTGGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIPRL ( NM_152902.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_690866.1 Homo sapiens TOR signaling pathway regulator (TIPRL), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu09534
Clone ID Related Accession (Same CDS sequence)	NM_152902.5 , NM_152902.4
Accession Version	NM_152902.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	TIP41-like protein isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK313520.1 and AL021397.1. On Dec 11, 2013 this sequence version replaced NM_152902.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK313520.1, SRR1803612.50197.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGATCC ACGGCTTCCA GAGCAGCCAC CGGGATTTCT GCTTCGGGCC CTGGAAGCTG 
ACGGCGTCCA AGACCCACAT CATGAAGTCG GCGGATGTGG AGAAATTAGC CGATGAATTA 
CATATGCCAT CTCTCCCTGA AATGATGTTT GGAGACAACG TTTTAAGAAT CCAGCATGGG 
TCTGGCTTTG GAATTGAGTT CAATGCTACA GATGCGTTAA GATGTGTAAA CAACTACCAA 
GGAATGCTTA AAGTGGCCTG TGCTGAAGAG TGGCAAGAAA GCAGGACGGA GGGTGAACAC 
TCCAAAGAGG TTATTAAACC ATATGATTGG ACCTATACAA CAGATTATAA GGGAACCTTA 
CTTGGAGAAT CTCTTAAGTT AAAGGTTGTA CCTACAACAG ATCATATAGA TACAGAAAAA 
TTGAAAGCCA GAGAACAGAT TAAGTTTTTT GAAGAAGTTC TCCTTTTTGA GGATGAACTT 
CATGATCATG GAGTTTCAAG CCTGAGTGTG AAGATTAGAG TAATGCCTTC TAGCTTTTTC 
CTGCTGTTGC GGTTTTTCTT GAGAATTGAT GGGGTGCTTA TCAGAATGAA TGACACGAGA 
CTTTACCATG AGGCTGACAA GACCTACATG TTACGAGAAT ATACGTCACG AGAAAGCAAA 
ATTTCTAGTT TGATGCATGT TCCACCTTCC CTCTTCACGG AACCTAATGA AATATCCCAG 
TATTTACCAA TAAAGGAAGC AGTTTGTGAG AAGCTAATAT TTCCAGAAAG AATTGATCCT 
AACCCAGCAG ACTCACAAAA AAGTACACAA GTGGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_690866.1
CDS	234..1052
Translation	MMIHGFQSSHRDFCFGPWKLTASKTHIMKSADVEKLADELHMPSLPEMMFGDNVLRIQHGSGFGIEFNATDALRCVNNYQGMLKVACAEEWQESRTEGEHSKEVIKPYDWTYTTDYKGTLLGESLKLKVVPTTDHIDTEKLKAREQIKFFEEVLLFEDELHDHGVSSLSVKIRVMPSSFFLLLRFFLRIDGVLIRMNDTRLYHEADKTYMLREYTSRESKISSLMHVPPSLFTEPNEISQYLPIKEAVCEKLIFPERIDPNPADSQKSTQVE

Target ORF information:

RefSeq Version	NM_152902.4
Organism	Homo sapiens(human)
Definition	Homo sapiens TOR signaling pathway regulator (TIPRL), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152902.4

ORF Insert Sequence:

ATGATGATCC ACGGCTTCCA GAGCAGCCAC CGGGATTTCT GCTTCGGGCC CTGGAAGCTG 
ACGGCGTCCA AGACCCACAT CATGAAGTCG GCGGATGTGG AGAAATTAGC CGATGAATTA 
CATATGCCAT CTCTCCCTGA AATGATGTTT GGAGACAACG TTTTAAGAAT CCAGCATGGG 
TCTGGCTTTG GAATTGAGTT CAATGCTACA GATGCGTTAA GATGTGTAAA CAACTACCAA 
GGAATGCTTA AAGTGGCCTG TGCTGAAGAG TGGCAAGAAA GCAGGACGGA GGGTGAACAC 
TCCAAAGAGG TTATTAAACC ATATGATTGG ACCTATACAA CAGATTATAA GGGAACCTTA 
CTTGGAGAAT CTCTTAAGTT AAAGGTTGTA CCTACAACAG ATCATATAGA TACAGAAAAA 
TTGAAAGCCA GAGAACAGAT TAAGTTTTTT GAAGAAGTTC TCCTTTTTGA GGATGAACTT 
CATGATCATG GAGTTTCAAG CCTGAGTGTG AAGATTAGAG TAATGCCTTC TAGCTTTTTC 
CTGCTGTTGC GGTTTTTCTT GAGAATTGAT GGGGTGCTTA TCAGAATGAA TGACACGAGA 
CTTTACCATG AGGCTGACAA GACCTACATG TTACGAGAAT ATACGTCACG AGAAAGCAAA 
ATTTCTAGTT TGATGCATGT TCCACCTTCC CTCTTCACGG AACCTAATGA AATATCCCAG 
TATTTACCAA TAAAGGAAGC AGTTTGTGAG AAGCTAATAT TTCCAGAAAG AATTGATCCT 
AACCCAGCAG ACTCACAAAA AAGTACACAA GTGGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TIPRL Inhibits Protein Phosphatase 4 Activity and Promotes H2AX Phosphorylation in the DNA Damage Response.
PloS one10(12)e0145938(2015)
Rosales KR,Reid MA,Yang Y,Tran TQ,Wang WI,Lowman X,Pan M,Kong M

Protein interaction network of the mammalian Hippo pathway reveals mechanisms of kinase-phosphatase interactions.
Science signaling6(302)rs15(2013 Nov)
Couzens AL,Knight JD,Kean MJ,Teo G,Weiss A,Dunham WH,Lin ZY,Bagshaw RD,Sicheri F,Pawson T,Wrana JL,Choi H,Gingras AC

A positive role of mammalian Tip41-like protein, TIPRL, in the amino-acid dependent mTORC1-signaling pathway through interaction with PP2A.
FEBS letters587(18)2924-9(2013 Sep)
Nakashima A,Tanimura-Ito K,Oshiro N,Eguchi S,Miyamoto T,Momonami A,Kamada S,Yonezawa K,Kikkawa U

Inhibition of MKK7-JNK by the TOR signaling pathway regulator-like protein contributes to resistance of HCC cells to TRAIL-induced apoptosis.
Gastroenterology143(5)1341-51(2012 Nov)
Song IS,Jun SY,Na HJ,Kim HT,Jung SY,Ha GH,Park YH,Long LZ,Yu DY,Kim JM,Kim JH,Ko JH,Kim CH,Kim NS