NPB cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NPB
Entrez Gene ID	256933
Full Name	neuropeptide B
Synonyms	L7,PPL7,PPNPB
General protein information	Preferred Names neuropeptide B Names neuropeptide B prepro-NPB preproneuropeptide B preproprotein L7
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q25.3
Summary	This gene encodes a member of the neuropeptide B/W family of proteins and preproprotein that is proteolytically processed to generate multiple protein products. The encoded products include neuropeptide B-23 and a C-terminally extended form, neuropeptide B-29, which are characterized by an N-terminal brominated tryptophan amino acid. Both of the encoded peptides bind with higher affinity to neuropeptide B/W (NPB/W) receptor 1 compared to the related NPB/W receptor 2. These peptides may regulate feeding, pain perception, and stress in rodents. [provided by RefSeq, Jul 2015].
Disorder MIM:	607996

mRNA and Protein(s)

mRNA	Protein	Name
NM_148896.5	NP_683694.1	neuropeptide B preproprotein
NM_148896.4	NP_683694.1	neuropeptide B preproprotein

Pathways from BioSystems

Homologies

	NPB	XP_001112472.1
Bos taurus (cattle)	NPB	NP_776369.1
Mus musculus (house mouse)	Npb	NP_695020.1
Rattus norvegicus (Norway rat)	Npb	NP_695205.1
Homo sapiens (human)	NPB	NP_683694.1
Canis lupus familiaris (dog)	NPB	XP_003639281.1

Gene Ontology
Bibliography

Related articles in PubMed

Molecular dynamics simulation of neuropeptide B and neuropeptide W in the dipalmitoylphosphatidylcholine membrane bilayer.
Patra MC, Maharana J, Pradhan SK, Rath SN
Journal of biomolecular structure & dynamics32(7)1118-31(2014)

NPBWR1 and NPBWR2: Implications in Energy Homeostasis, Pain, and Emotion.
Sakurai T
Frontiers in endocrinology423(2013)

Development of a potent and selective GPR7 (NPBW1) agonist: a systematic structure-activity study of neuropeptide B.
Kanesaka M, Matsuda M, Hirano A, Tanaka K, Kanatani A, Tokita S
Journal of peptide science : an official publication of the European Peptide Society13(6)379-85(2007 Jun)

Neuropeptide B and W: neurotransmitters in an emerging G-protein-coupled receptor system.
Singh G, Davenport AP
British journal of pharmacology148(8)1033-41(2006 Aug)

Neuropeptides B and W enhance the growth of human adrenocortical carcinoma-derived NCI-H295 cells by exerting MAPK p42/p44-mediated proliferogenic and antiapoptotic effects.
Andreis PG, Rucinski M, Neri G, Conconi MT, Petrelli L, Parnigotto PP, Malendowicz LK, Nussdorfer GG
International journal of molecular medicine16(6)1021-8(2005 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

identified a neuropeptide modified with bromine; an endogenous ligand for GPR7
Title: Identification of a neuropeptide modified with bromine as an endogenous ligand for GPR7.

The following NPB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NPB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31411	NM_148896.5 Latest version!	Homo sapiens neuropeptide B (NPB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
View Old Accession Versions >>
OHu31411	NM_148896.4	Homo sapiens neuropeptide B (NPB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NPB ( NM_148896.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_683694.1 Homo sapiens neuropeptide B (NPB), mRNA.

Price & Availability↑

CloneID	OHu31411
Clone ID Related Accession (Same CDS sequence)	NM_148896.5 , NM_148896.4
Accession Version	NM_148896.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	neuropeptide B preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC126128.1 and BM978256.1. On Nov 22, 2018 this sequence version replaced NM_148896.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BM978256.1, SRR1803612.301958.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000333383.8/ ENSP00000332766.7 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCCGGT CCGCGACACT GGCGGCCGCC GCCCTGGCGC TGTGCCTGCT GCTGGCGCCG 
CCTGGCCTCG CGTGGTACAA GCCAGCGGCG GGGCACAGCT CCTACTCGGT GGGCCGCGCC 
GCGGGGCTGC TGTCCGGCCT CCGCAGGTCC CCGTACGCGC GGCGCTCCCA GCCCTACAGA 
GGGGCGGAAC CCCCGGGCGG GGCCGGCGCC TCCCCGGAGC TGCAACTGCA CCCCAGGCTG 
CGGAGCCTCG CTGTGTGCGT CCAGGACGTC GCCCCAAACC TGCAGAGGTG CGAGCGGCTC 
CCCGACGGCC GCGGGACCTA CCAGTGCAAG GCGAACGTCT TCCTGTCCCT GCGCGCAGCC 
GACTGCCTCG CCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_683694.1
CDS	41..418
Translation	MARSATLAAAALALCLLLAPPGLAWYKPAAGHSSYSVGRAAGLLSGLRRSPYARRSQPYRGAEPPGGAGASPELQLHPRLRSLAVCVQDVAPNLQRCERLPDGRGTYQCKANVFLSLRAADCLAA

Target ORF information:

RefSeq Version	NM_148896.5
Organism	Homo sapiens(human)
Definition	Homo sapiens neuropeptide B (NPB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_148896.5

ORF Insert Sequence:

ATGGCCCGGT CCGCGACACT GGCGGCCGCC GCCCTGGCGC TGTGCCTGCT GCTGGCGCCG 
CCTGGCCTCG CGTGGTACAA GCCAGCGGCG GGGCACAGCT CCTACTCGGT GGGCCGCGCC 
GCGGGGCTGC TGTCCGGCCT CCGCAGGTCC CCGTACGCGC GGCGCTCCCA GCCCTACAGA 
GGGGCGGAAC CCCCGGGCGG GGCCGGCGCC TCCCCGGAGC TGCAACTGCA CCCCAGGCTG 
CGGAGCCTCG CTGTGTGCGT CCAGGACGTC GCCCCAAACC TGCAGAGGTG CGAGCGGCTC 
CCCGACGGCC GCGGGACCTA CCAGTGCAAG GCGAACGTCT TCCTGTCCCT GCGCGCAGCC 
GACTGCCTCG CCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPB ( NM_148896.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_683694.1 Homo sapiens neuropeptide B (NPB), mRNA.

Price & Availability↑

CloneID	OHu31411
Clone ID Related Accession (Same CDS sequence)	NM_148896.5 , NM_148896.4
Accession Version	NM_148896.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	neuropeptide B preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM978256.1, AC145207.2 and BC126128.1. On Aug 1, 2015 this sequence version replaced NM_148896.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCGGT CCGCGACACT GGCGGCCGCC GCCCTGGCGC TGTGCCTGCT GCTGGCGCCG 
CCTGGCCTCG CGTGGTACAA GCCAGCGGCG GGGCACAGCT CCTACTCGGT GGGCCGCGCC 
GCGGGGCTGC TGTCCGGCCT CCGCAGGTCC CCGTACGCGC GGCGCTCCCA GCCCTACAGA 
GGGGCGGAAC CCCCGGGCGG GGCCGGCGCC TCCCCGGAGC TGCAACTGCA CCCCAGGCTG 
CGGAGCCTCG CTGTGTGCGT CCAGGACGTC GCCCCAAACC TGCAGAGGTG CGAGCGGCTC 
CCCGACGGCC GCGGGACCTA CCAGTGCAAG GCGAACGTCT TCCTGTCCCT GCGCGCAGCC 
GACTGCCTCG CCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_683694.1
CDS	170..547
Translation	MARSATLAAAALALCLLLAPPGLAWYKPAAGHSSYSVGRAAGLLSGLRRSPYARRSQPYRGAEPPGGAGASPELQLHPRLRSLAVCVQDVAPNLQRCERLPDGRGTYQCKANVFLSLRAADCLAA

Target ORF information:

RefSeq Version	NM_148896.4
Organism	Homo sapiens(human)
Definition	Homo sapiens neuropeptide B (NPB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_148896.4

ORF Insert Sequence:

ATGGCCCGGT CCGCGACACT GGCGGCCGCC GCCCTGGCGC TGTGCCTGCT GCTGGCGCCG 
CCTGGCCTCG CGTGGTACAA GCCAGCGGCG GGGCACAGCT CCTACTCGGT GGGCCGCGCC 
GCGGGGCTGC TGTCCGGCCT CCGCAGGTCC CCGTACGCGC GGCGCTCCCA GCCCTACAGA 
GGGGCGGAAC CCCCGGGCGG GGCCGGCGCC TCCCCGGAGC TGCAACTGCA CCCCAGGCTG 
CGGAGCCTCG CTGTGTGCGT CCAGGACGTC GCCCCAAACC TGCAGAGGTG CGAGCGGCTC 
CCCGACGGCC GCGGGACCTA CCAGTGCAAG GCGAACGTCT TCCTGTCCCT GCGCGCAGCC 
GACTGCCTCG CCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Molecular dynamics simulation of neuropeptide B and neuropeptide W in the dipalmitoylphosphatidylcholine membrane bilayer.
Journal of biomolecular structure & dynamics32(7)1118-31(2014)
Patra MC,Maharana J,Pradhan SK,Rath SN

NPBWR1 and NPBWR2: Implications in Energy Homeostasis, Pain, and Emotion.
Frontiers in endocrinology423(2013)
Sakurai T

Development of a potent and selective GPR7 (NPBW1) agonist: a systematic structure-activity study of neuropeptide B.
Journal of peptide science : an official publication of the European Peptide Society13(6)379-85(2007 Jun)
Kanesaka M,Matsuda M,Hirano A,Tanaka K,Kanatani A,Tokita S

Neuropeptide B and W: neurotransmitters in an emerging G-protein-coupled receptor system.
British journal of pharmacology148(8)1033-41(2006 Aug)
Singh G,Davenport AP

Neuropeptides B and W enhance the growth of human adrenocortical carcinoma-derived NCI-H295 cells by exerting MAPK p42/p44-mediated proliferogenic and antiapoptotic effects.
International journal of molecular medicine16(6)1021-8(2005 Dec)
Andreis PG,Rucinski M,Neri G,Conconi MT,Petrelli L,Parnigotto PP,Malendowicz LK,Nussdorfer GG