DEFB105A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DEFB105A
Entrez Gene ID	245908
Full Name	defensin beta 105A
Synonyms	BD-5,DEFB-5,DEFB105
General protein information	Preferred Names defensin beta 105A Names beta-defensin 105 beta defensin 5 defensin, beta 5
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 8 Map Location: 8p23.1
Summary	Defensins form a family of antimicrobial and cytotoxic peptides made by neutrophils. Defensins are short, processed peptide molecules that are classified by structure into three groups: alpha-defensins, beta-defensins and theta-defensins. All beta-defensin genes are densely clustered in four to five syntenic chromosomal regions. Chromosome 8p23 contains at least two copies of the duplicated beta-defensin cluster. This duplication results in two identical copies of defensin, beta 105, DEFB105A and DEFB105B, in tail-to-tail orientation. This gene, DEFB105A, represents the more centromeric copy. [provided by RefSeq, Oct 2014].

mRNA and Protein(s)

mRNA	Protein	Name
NM_152250.2	NP_689463.1	beta-defensin 105 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Defb12	NP_690015.1
Canis lupus familiaris (dog)	DEFB105A	NP_001158731.1
Bos taurus (cattle)	LOC100296624	XP_002698668.1
Rattus norvegicus (Norway rat)	Defb12	NP_001032603.1
Homo sapiens (human)	DEFB105A	NP_689463.1
Homo sapiens (human)	DEFB105B	NP_001035793.1
Pan troglodytes (chimpanzee)	DEFB105A	NP_001123225.1
	LOC694797	XP_001083455.1

Gene Ontology
Bibliography

Related articles in PubMed

Human defensin 5 disulfide array mutants: disulfide bond deletion attenuates antibacterial activity against Staphylococcus aureus.
Wanniarachchi YA, Kaczmarek P, Wan A, Nolan EM
Biochemistry50(37)8005-17(2011 Sep)

Copy number variation of defensin genes and HIV infection in Brazilian children.
Milanese M, Segat L, Arraes LC, Garzino-Demo A, Crovella S
Journal of acquired immune deficiency syndromes (1999)50(3)331-3(2009 Mar)

Cross-species analysis of the mammalian beta-defensin gene family: presence of syntenic gene clusters and preferential expression in the male reproductive tract.
Patil AA, Cai Y, Sang Y, Blecha F, Zhang G
Physiological genomics23(1)5-17(2005 Sep)

Polymorphic segmental duplications at 8p23.1 challenge the determination of individual defensin gene repertoires and the assembly of a contiguous human reference sequence.
Taudien S, Galgoczy P, Huse K, Reichwald K, Schilhabel M, Szafranski K, Shimizu A, Asakawa S, Frankish A, Loncarevic IF, Shimizu N, Siddiqui R, Platzer M
BMC genomics5(1)92(2004 Dec)

Evidence for duplication of the human defensin gene DEFB4 in chromosomal region 8p22-23 and implications for the analysis of SNP allele distribution.
Boniotto M, Ventura M, Eskdale J, Crovella S, Gallagher G
Genetic testing8(3)325-7(2004)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Defensin-beta 105 displays antimicrobial activity against S. aureus and E. coli, among other bacteria.
Title: Human defensin 5 disulfide array mutants: disulfide bond deletion attenuates antibacterial activity against Staphylococcus aureus.

Observational study of gene-disease association. (HuGE Navigator)
Title: Copy number variation of defensin genes and HIV infection in Brazilian children.

The epididymis-specific expression pattern of the beta-defensin isoform BD-5 has been characterized.
Title: Identification of multiple novel epididymis-specific beta-defensin isoforms in humans and mice.

The following DEFB105A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DEFB105A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu15518	NM_152250.2 Latest version!	Homo sapiens defensin beta 105A (DEFB105A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DEFB105A ( NM_152250.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_689463.1 Homo sapiens defensin beta 105A (DEFB105A), mRNA.

Price & Availability↑

CloneID	OHu15518
Clone ID Related Accession (Same CDS sequence)	NM_152250.2
Accession Version	NM_152250.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-30
Organism	Homo sapiens(human)
Product	beta-defensin 105 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC084121.13 and AB089180.1. On Apr 12, 2014 this sequence version replaced NM_152250.1. ##Evidence-Data-START## Transcript exon combination :: SRR5189667.141211.1, SRR5189667.178242.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2162841 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## Protein has antimicrobial activity :: PMID: 21861459 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##

ATGGCCCTGA TCAGGAAGAC ATTTTATTTT CTATTTGCTA TGTTCTTCAT TTTGGTTCAA 
CTGCCATCAG GGTGCCAGGC AGGACTTGAT TTTTCCCAAC CATTTCCATC AGGTGAGTTT 
GCTGTCTGTG AGTCGTGCAA GCTTGGTCGG GGAAAATGCA GGAAGGAGTG CTTGGAGAAT 
GAGAAGCCCG ATGGAAATTG CAGGCTGAAC TTTCTCTGCT GCAGACAGAG GATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_689463.1
CDS	53..289
Translation	MALIRKTFYFLFAMFFILVQLPSGCQAGLDFSQPFPSGEFAVCESCKLGRGKCRKECLENEKPDGNCRLNFLCCRQRI

Target ORF information:

RefSeq Version	NM_152250.2
Organism	Homo sapiens(human)
Definition	Homo sapiens defensin beta 105A (DEFB105A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152250.2

ORF Insert Sequence:

ATGGCCCTGA TCAGGAAGAC ATTTTATTTT CTATTTGCTA TGTTCTTCAT TTTGGTTCAA 
CTGCCATCAG GGTGCCAGGC AGGACTTGAT TTTTCCCAAC CATTTCCATC AGGTGAGTTT 
GCTGTCTGTG AGTCGTGCAA GCTTGGTCGG GGAAAATGCA GGAAGGAGTG CTTGGAGAAT 
GAGAAGCCCG ATGGAAATTG CAGGCTGAAC TTTCTCTGCT GCAGACAGAG GATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Human defensin 5 disulfide array mutants: disulfide bond deletion attenuates antibacterial activity against Staphylococcus aureus.
Biochemistry50(37)8005-17(2011 Sep)
Wanniarachchi YA,Kaczmarek P,Wan A,Nolan EM

Copy number variation of defensin genes and HIV infection in Brazilian children.
Journal of acquired immune deficiency syndromes (1999)50(3)331-3(2009 Mar)
Milanese M,Segat L,Arraes LC,Garzino-Demo A,Crovella S

Cross-species analysis of the mammalian beta-defensin gene family: presence of syntenic gene clusters and preferential expression in the male reproductive tract.
Physiological genomics23(1)5-17(2005 Sep)
Patil AA,Cai Y,Sang Y,Blecha F,Zhang G

Polymorphic segmental duplications at 8p23.1 challenge the determination of individual defensin gene repertoires and the assembly of a contiguous human reference sequence.
BMC genomics5(1)92(2004 Dec)
Taudien S,Galgoczy P,Huse K,Reichwald K,Schilhabel M,Szafranski K,Shimizu A,Asakawa S,Frankish A,Loncarevic IF,Shimizu N,Siddiqui R,Platzer M

Evidence for duplication of the human defensin gene DEFB4 in chromosomal region 8p22-23 and implications for the analysis of SNP allele distribution.
Genetic testing8(3)325-7(2004)
Boniotto M,Ventura M,Eskdale J,Crovella S,Gallagher G