EMC1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_001271428.2	NP_001258357.1	ER membrane protein complex subunit 1 isoform 3 precursor
NM_001375821.1	NP_001362750.1	ER membrane protein complex subunit 1 isoform 6 precursor
XM_005245787.1	XP_005245844.1	ER membrane protein complex subunit 1 isoform X1
NM_001271429.1	NP_001258358.1	ER membrane protein complex subunit 1 isoform 4 precursor
NM_001271429.2	NP_001258358.1	ER membrane protein complex subunit 1 isoform 4 precursor
NM_001375820.1	NP_001362749.1	ER membrane protein complex subunit 1 isoform 5 precursor
XM_005245788.1	XP_005245845.1	ER membrane protein complex subunit 1 isoform X2
NM_015047.2	NP_055862.1	ER membrane protein complex subunit 1 isoform 1 precursor
NM_001271427.2	NP_001258356.1	ER membrane protein complex subunit 1 isoform 2 precursor
NM_015047.3	NP_055862.1	ER membrane protein complex subunit 1 isoform 1 precursor
XM_005245787.2	XP_005245844.1	ER membrane protein complex subunit 1 isoform X1
XM_005245788.2	XP_005245845.1	ER membrane protein complex subunit 1 isoform X2
NM_001271427.1	NP_001258356.1	ER membrane protein complex subunit 1 isoform 2 precursor
NM_001271428.1	NP_001258357.1	ER membrane protein complex subunit 1 isoform 3 precursor

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22039	NM_015047.3 Latest version!	Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu10222	NM_001271427.2 Latest version!	Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu11738	NM_001271428.2 Latest version!	Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu12432	NM_001271429.2 Latest version!	Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu33200	XM_005245787.2 Latest version!	Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu33201	XM_005245788.2 Latest version!	Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu33200	NM_001375820.1 Latest version!	Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu33201	NM_001375821.1 Latest version!	Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OHu12432	NM_001271429.1	Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu22039	NM_015047.2	Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu33200	XM_005245787.1	Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu33201	XM_005245788.1	Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu10222	NM_001271427.1	Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu11738	NM_001271428.1	Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

EMC1 ( NM_015047.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_055862.1 Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22039
Clone ID Related Accession (Same CDS sequence)	NM_015047.2 , NM_015047.3
Accession Version	NM_015047.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2982bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-02
Organism	Homo sapiens(human)
Product	ER membrane protein complex subunit 1 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB097686.1, BC034589.1, AL357564.17 and BX648708.1. This sequence is a reference standard in the RefSeqGene project. On Sep 25, 2012 this sequence version replaced NM_015047.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.98359.1, SRR1803613.213547.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGCTG AGTGGGCTTC TCGTTTCTGG CTTTGGGCTA CGCTGCTGAT TCCTGCGGCC 
GCGGTCTACG AAGACCAAGT GGGCAAGTTT GATTGGAGAC AGCAATATGT TGGGAAGGTC 
AAGTTTGCCT CCTTGGAATT TTCCCCTGGA TCCAAGAAGT TGGTTGTAGC CACAGAGAAG 
AATGTGATTG CAGCATTAAA TTCCCGAACT GGGGAGATCT TGTGGCGCCA TGTTGACAAG 
GGCACGGCAG AAGGGGCTGT GGATGCCATG CTGCTGCACG GACAGGATGT GATCACTGTG 
TCCAATGGAG GCCGAATCAT GCGTTCCTGG GAGACTAACA TCGGGGGCCT GAACTGGGAG 
ATAACCCTGG ACAGTGGCAG TTTCCAGGCA CTTGGGCTGG TTGGCCTGCA GGAGTCTGTA 
AGGTACATCG CAGTCCTGAA GAAGACTACA CTTGCCCTCC ATCACCTCTC CAGTGGGCAC 
CTCAAGTGGG TGGAACATCT CCCAGAAAGT GACAGCATCC ACTACCAGAT GGTGTATTCT 
TACGGCTCTG GGGTGGTGTG GGCCCTCGGA GTTGTTCCCT TCAGCCATGT GAACATTGTC 
AAGTTTAATG TGGAAGATGG AGAGATTGTT CAGCAGGTTA GGGTTTCAAC TCCGTGGCTG 
CAGCACCTGT CTGGAGCCTG TGGTGTGGTG GATGAGGCTG TCCTGGTGTG TCCTGACCCG 
AGCTCACGTT CCCTCCAAAC TTTGGCTCTG GAGACGGAAT GGGAGTTGAG ACAGATCCCA 
CTGCAGTCTC TCGACTTAGA ATTTGGAAGT GGATTCCAAC CCCGGGTCCT GCCTACCCAG 
CCCAACCCAG TGGACGCTTC CCGGGCCCAG TTCTTCCTGC ACTTGTCCCC AAGCCACTAT 
GCTCTGCTGC AGTACCATTA TGGAACGCTG AGTTTGCTTA AAAACTTCCC ACAGACTGCC 
CTAGTGAGCT TTGCCACCAC TGGGGAGAAG ACGGTGGCTG CAGTCATGGC CTGTCGGAAT 
GAAGTGCAGA AAAGTAGCAG TTCTGAAGAT GGGTCAATGG GGAGCTTTTC GGAGAAGTCT 
AGTTCAAAGG ACTCTCTGGC TTGCTTCAAT CAGACCTACA CCATTAACCT ATACCTCGTG 
GAGACAGGTC GGCGGCTGCT GGACACCACG ATAACATTTA GCCTGGAACA GAGCGGCACT 
CGGCCTGAGC GGCTGTATAT CCAGGTGTTC TTGAAGAAGG ATGACTCAGT GGGCTACCGG 
GCTTTGGTGC AGACAGAGGA TCATCTGCTA CTTTTCCTGC AGCAGTTGGC AGGGAAGGTG 
GTGCTGTGGA GCCGTGAGGA GTCCCTGGCA GAAGTGGTGT GCCTAGAGAT GGTGGACCTC 
CCCCTGACTG GGGCACAGGC CGAGCTGGAA GGAGAATTTG GCAAAAAGGC AGATGGCTTG 
CTGGGGATGT TCCTGAAACG CCTCTCGTCT CAGCTTATCC TGCTGCAAGC ATGGACTTCC 
CACCTCTGGA AAATGTTTTA TGATGCTCGG AAGCCCCGGA GTCAGATTAA GAATGAGATC 
AACATTGACA CCCTGGCCAG AGATGAATTC AACCTCCAGA AGATGATGGT GATGGTAACA 
GCCTCAGGCA AGCTTTTTGG CATTGAGAGC AGCTCTGGCA CCATCCTGTG GAAACAGTAT 
CTACCCAATG TCAAGCCAGA CTCCTCCTTT AAACTGATGG TCCAGAGAAC TACTGCTCAT 
TTCCCCCATC CCCCACAGTG CACCCTGCTG GTGAAGGACA AGGAGTCGGG AATGAGTTCT 
CTGTATGTCT TCAATCCCAT TTTTGGGAAG TGGAGTCAGG TAGCTCCCCC AGTGCTGAAG 
CGCCCCATCT TGCAGTCCTT GCTTCTCCCA GTCATGGATC AAGACTACGC CAAGGTGTTG 
CTGTTGATAG ATGATGAATA CAAGGTCACA GCTTTTCCAG CCACTCGGAA TGTCTTGCGA 
CAGCTACATG AGCTTGCCCC TTCCATCTTC TTCTATTTGG TGGATGCAGA GCAGGGACGG 
CTGTGTGGAT ATCGGCTTCG AAAGGATCTC ACCACTGAGC TGAGTTGGGA GCTGACCATT 
CCCCCAGAAG TACAGCGGAT CGTCAAGGTG AAGGGGAAAC GCAGCAGTGA GCACGTTCAT 
TCCCAGGGCC GTGTGATGGG GGACCGCAGT GTGCTCTACA AGAGCCTGAA CCCCAACCTG 
CTGGCCGTGG TGACAGAGAG CACAGACGCG CACCATGAGC GCACCTTTAT TGGCATCTTC 
CTCATTGATG GCGTCACTGG GCGTATCATT CACTCCTCTG TGCAGAAGAA AGCCAAAGGC 
CCTGTCCATA TCGTGCATTC AGAGAACTGG GTGGTGTACC AGTACTGGAA CACCAAGGCT 
CGGCGCAACG AGTTTACCGT ACTGGAGCTC TATGAGGGCA CTGAGCAATA CAACGCCACC 
GCCTTCAGCT CCCTGGACCG CCCCCAGCTG CCCCAGGTCC TCCAGCAGTC CTATATCTTC 
CCGTCCTCCA TCAGTGCCAT GGAGGCCACC ATCACCGAAC GGGGCATCAC CAGCCGACAC 
CTGCTGATTG GACTACCTTC TGGAGCAATT CTTTCCCTTC CTAAGGCTTT GCTGGATCCC 
CGCCGCCCCG AGATCCCAAC AGAACAAAGC AGAGAGGAGA ACTTAATCCC GTATTCTCCA 
GATGTACAGA TACACGCAGA GCGATTCATC AACTATAACC AGACAGTTTC TCGAATGCGA 
GGTATCTACA CAGCTCCCTC GGGTCTGGAG TCCACTTGTT TGGTTGTGGC CTATGGTTTG 
GACATTTACC AAACTCGAGT CTACCCATCC AAGCAGTTTG ACGTTCTGAA GGATGACTAT 
GACTACGTGT TAATCAGCAG CGTCCTCTTT GGCCTGGTTT TTGCCACCAT GATCACTAAG 
AGACTGGCAC AGGTGAAGCT CCTGAATCGG GCCTGGCGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055862.1
CDS	51..3032
Translation	MAAEWASRFWLWATLLIPAAAVYEDQVGKFDWRQQYVGKVKFASLEFSPGSKKLVVATEKNVIAALNSRTGEILWRHVDKGTAEGAVDAMLLHGQDVITVSNGGRIMRSWETNIGGLNWEITLDSGSFQALGLVGLQESVRYIAVLKKTTLALHHLSSGHLKWVEHLPESDSIHYQMVYSYGSGVVWALGVVPFSHVNIVKFNVEDGEIVQQVRVSTPWLQHLSGACGVVDEAVLVCPDPSSRSLQTLALETEWELRQIPLQSLDLEFGSGFQPRVLPTQPNPVDASRAQFFLHLSPSHYALLQYHYGTLSLLKNFPQTALVSFATTGEKTVAAVMACRNEVQKSSSSEDGSMGSFSEKSSSKDSLACFNQTYTINLYLVETGRRLLDTTITFSLEQSGTRPERLYIQVFLKKDDSVGYRALVQTEDHLLLFLQQLAGKVVLWSREESLAEVVCLEMVDLPLTGAQAELEGEFGKKADGLLGMFLKRLSSQLILLQAWTSHLWKMFYDARKPRSQIKNEINIDTLARDEFNLQKMMVMVTASGKLFGIESSSGTILWKQYLPNVKPDSSFKLMVQRTTAHFPHPPQCTLLVKDKESGMSSLYVFNPIFGKWSQVAPPVLKRPILQSLLLPVMDQDYAKVLLLIDDEYKVTAFPATRNVLRQLHELAPSIFFYLVDAEQGRLCGYRLRKDLTTELSWELTIPPEVQRIVKVKGKRSSEHVHSQGRVMGDRSVLYKSLNPNLLAVVTESTDAHHERTFIGIFLIDGVTGRIIHSSVQKKAKGPVHIVHSENWVVYQYWNTKARRNEFTVLELYEGTEQYNATAFSSLDRPQLPQVLQQSYIFPSSISAMEATITERGITSRHLLIGLPSGAILSLPKALLDPRRPEIPTEQSREENLIPYSPDVQIHAERFINYNQTVSRMRGIYTAPSGLESTCLVVAYGLDIYQTRVYPSKQFDVLKDDYDYVLISSVLFGLVFATMITKRLAQVKLLNRAWR

Target ORF information:

RefSeq Version	NM_015047.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015047.2

ORF Insert Sequence:

ATGGCGGCTG AGTGGGCTTC TCGTTTCTGG CTTTGGGCTA CGCTGCTGAT TCCTGCGGCC 
GCGGTCTACG AAGACCAAGT GGGCAAGTTT GATTGGAGAC AGCAATATGT TGGGAAGGTC 
AAGTTTGCCT CCTTGGAATT TTCCCCTGGA TCCAAGAAGT TGGTTGTAGC CACAGAGAAG 
AATGTGATTG CAGCATTAAA TTCCCGAACT GGGGAGATCT TGTGGCGCCA TGTTGACAAG 
GGCACGGCAG AAGGGGCTGT GGATGCCATG CTGCTGCACG GACAGGATGT GATCACTGTG 
TCCAATGGAG GCCGAATCAT GCGTTCCTGG GAGACTAACA TCGGGGGCCT GAACTGGGAG 
ATAACCCTGG ACAGTGGCAG TTTCCAGGCA CTTGGGCTGG TTGGCCTGCA GGAGTCTGTA 
AGGTACATCG CAGTCCTGAA GAAGACTACA CTTGCCCTCC ATCACCTCTC CAGTGGGCAC 
CTCAAGTGGG TGGAACATCT CCCAGAAAGT GACAGCATCC ACTACCAGAT GGTGTATTCT 
TACGGCTCTG GGGTGGTGTG GGCCCTCGGA GTTGTTCCCT TCAGCCATGT GAACATTGTC 
AAGTTTAATG TGGAAGATGG AGAGATTGTT CAGCAGGTTA GGGTTTCAAC TCCGTGGCTG 
CAGCACCTGT CTGGAGCCTG TGGTGTGGTG GATGAGGCTG TCCTGGTGTG TCCTGACCCG 
AGCTCACGTT CCCTCCAAAC TTTGGCTCTG GAGACGGAAT GGGAGTTGAG ACAGATCCCA 
CTGCAGTCTC TCGACTTAGA ATTTGGAAGT GGATTCCAAC CCCGGGTCCT GCCTACCCAG 
CCCAACCCAG TGGACGCTTC CCGGGCCCAG TTCTTCCTGC ACTTGTCCCC AAGCCACTAT 
GCTCTGCTGC AGTACCATTA TGGAACGCTG AGTTTGCTTA AAAACTTCCC ACAGACTGCC 
CTAGTGAGCT TTGCCACCAC TGGGGAGAAG ACGGTGGCTG CAGTCATGGC CTGTCGGAAT 
GAAGTGCAGA AAAGTAGCAG TTCTGAAGAT GGGTCAATGG GGAGCTTTTC GGAGAAGTCT 
AGTTCAAAGG ACTCTCTGGC TTGCTTCAAT CAGACCTACA CCATTAACCT ATACCTCGTG 
GAGACAGGTC GGCGGCTGCT GGACACCACG ATAACATTTA GCCTGGAACA GAGCGGCACT 
CGGCCTGAGC GGCTGTATAT CCAGGTGTTC TTGAAGAAGG ATGACTCAGT GGGCTACCGG 
GCTTTGGTGC AGACAGAGGA TCATCTGCTA CTTTTCCTGC AGCAGTTGGC AGGGAAGGTG 
GTGCTGTGGA GCCGTGAGGA GTCCCTGGCA GAAGTGGTGT GCCTAGAGAT GGTGGACCTC 
CCCCTGACTG GGGCACAGGC CGAGCTGGAA GGAGAATTTG GCAAAAAGGC AGATGGCTTG 
CTGGGGATGT TCCTGAAACG CCTCTCGTCT CAGCTTATCC TGCTGCAAGC ATGGACTTCC 
CACCTCTGGA AAATGTTTTA TGATGCTCGG AAGCCCCGGA GTCAGATTAA GAATGAGATC 
AACATTGACA CCCTGGCCAG AGATGAATTC AACCTCCAGA AGATGATGGT GATGGTAACA 
GCCTCAGGCA AGCTTTTTGG CATTGAGAGC AGCTCTGGCA CCATCCTGTG GAAACAGTAT 
CTACCCAATG TCAAGCCAGA CTCCTCCTTT AAACTGATGG TCCAGAGAAC TACTGCTCAT 
TTCCCCCATC CCCCACAGTG CACCCTGCTG GTGAAGGACA AGGAGTCGGG AATGAGTTCT 
CTGTATGTCT TCAATCCCAT TTTTGGGAAG TGGAGTCAGG TAGCTCCCCC AGTGCTGAAG 
CGCCCCATCT TGCAGTCCTT GCTTCTCCCA GTCATGGATC AAGACTACGC CAAGGTGTTG 
CTGTTGATAG ATGATGAATA CAAGGTCACA GCTTTTCCAG CCACTCGGAA TGTCTTGCGA 
CAGCTACATG AGCTTGCCCC TTCCATCTTC TTCTATTTGG TGGATGCAGA GCAGGGACGG 
CTGTGTGGAT ATCGGCTTCG AAAGGATCTC ACCACTGAGC TGAGTTGGGA GCTGACCATT 
CCCCCAGAAG TACAGCGGAT CGTCAAGGTG AAGGGGAAAC GCAGCAGTGA GCACGTTCAT 
TCCCAGGGCC GTGTGATGGG GGACCGCAGT GTGCTCTACA AGAGCCTGAA CCCCAACCTG 
CTGGCCGTGG TGACAGAGAG CACAGACGCG CACCATGAGC GCACCTTTAT TGGCATCTTC 
CTCATTGATG GCGTCACTGG GCGTATCATT CACTCCTCTG TGCAGAAGAA AGCCAAAGGC 
CCTGTCCATA TCGTGCATTC AGAGAACTGG GTGGTGTACC AGTACTGGAA CACCAAGGCT 
CGGCGCAACG AGTTTACCGT ACTGGAGCTC TATGAGGGCA CTGAGCAATA CAACGCCACC 
GCCTTCAGCT CCCTGGACCG CCCCCAGCTG CCCCAGGTCC TCCAGCAGTC CTATATCTTC 
CCGTCCTCCA TCAGTGCCAT GGAGGCCACC ATCACCGAAC GGGGCATCAC CAGCCGACAC 
CTGCTGATTG GACTACCTTC TGGAGCAATT CTTTCCCTTC CTAAGGCTTT GCTGGATCCC 
CGCCGCCCCG AGATCCCAAC AGAACAAAGC AGAGAGGAGA ACTTAATCCC GTATTCTCCA 
GATGTACAGA TACACGCAGA GCGATTCATC AACTATAACC AGACAGTTTC TCGAATGCGA 
GGTATCTACA CAGCTCCCTC GGGTCTGGAG TCCACTTGTT TGGTTGTGGC CTATGGTTTG 
GACATTTACC AAACTCGAGT CTACCCATCC AAGCAGTTTG ACGTTCTGAA GGATGACTAT 
GACTACGTGT TAATCAGCAG CGTCCTCTTT GGCCTGGTTT TTGCCACCAT GATCACTAAG 
AGACTGGCAC AGGTGAAGCT CCTGAATCGG GCCTGGCGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMC1 ( NM_015047.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055862.1 Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22039
Clone ID Related Accession (Same CDS sequence)	NM_015047.2 , NM_015047.3
Accession Version	NM_015047.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2982bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	ER membrane protein complex subunit 1 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC034589.1, AL357564.17 and BX648708.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_015047.2. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.98359.1, SRR1803613.213547.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000477853.6/ ENSP00000420608.1 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGCTG AGTGGGCTTC TCGTTTCTGG CTTTGGGCTA CGCTGCTGAT TCCTGCGGCC 
GCGGTCTACG AAGACCAAGT GGGCAAGTTT GATTGGAGAC AGCAATATGT TGGGAAGGTC 
AAGTTTGCCT CCTTGGAATT TTCCCCTGGA TCCAAGAAGT TGGTTGTAGC CACAGAGAAG 
AATGTGATTG CAGCATTAAA TTCCCGAACT GGGGAGATCT TGTGGCGCCA TGTTGACAAG 
GGCACGGCAG AAGGGGCTGT GGATGCCATG CTGCTGCACG GACAGGATGT GATCACTGTG 
TCCAATGGAG GCCGAATCAT GCGTTCCTGG GAGACTAACA TCGGGGGCCT GAACTGGGAG 
ATAACCCTGG ACAGTGGCAG TTTCCAGGCA CTTGGGCTGG TTGGCCTGCA GGAGTCTGTA 
AGGTACATCG CAGTCCTGAA GAAGACTACA CTTGCCCTCC ATCACCTCTC CAGTGGGCAC 
CTCAAGTGGG TGGAACATCT CCCAGAAAGT GACAGCATCC ACTACCAGAT GGTGTATTCT 
TACGGCTCTG GGGTGGTGTG GGCCCTCGGA GTTGTTCCCT TCAGCCATGT GAACATTGTC 
AAGTTTAATG TGGAAGATGG AGAGATTGTT CAGCAGGTTA GGGTTTCAAC TCCGTGGCTG 
CAGCACCTGT CTGGAGCCTG TGGTGTGGTG GATGAGGCTG TCCTGGTGTG TCCTGACCCG 
AGCTCACGTT CCCTCCAAAC TTTGGCTCTG GAGACGGAAT GGGAGTTGAG ACAGATCCCA 
CTGCAGTCTC TCGACTTAGA ATTTGGAAGT GGATTCCAAC CCCGGGTCCT GCCTACCCAG 
CCCAACCCAG TGGACGCTTC CCGGGCCCAG TTCTTCCTGC ACTTGTCCCC AAGCCACTAT 
GCTCTGCTGC AGTACCATTA TGGAACGCTG AGTTTGCTTA AAAACTTCCC ACAGACTGCC 
CTAGTGAGCT TTGCCACCAC TGGGGAGAAG ACGGTGGCTG CAGTCATGGC CTGTCGGAAT 
GAAGTGCAGA AAAGTAGCAG TTCTGAAGAT GGGTCAATGG GGAGCTTTTC GGAGAAGTCT 
AGTTCAAAGG ACTCTCTGGC TTGCTTCAAT CAGACCTACA CCATTAACCT ATACCTCGTG 
GAGACAGGTC GGCGGCTGCT GGACACCACG ATAACATTTA GCCTGGAACA GAGCGGCACT 
CGGCCTGAGC GGCTGTATAT CCAGGTGTTC TTGAAGAAGG ATGACTCAGT GGGCTACCGG 
GCTTTGGTGC AGACAGAGGA TCATCTGCTA CTTTTCCTGC AGCAGTTGGC AGGGAAGGTG 
GTGCTGTGGA GCCGTGAGGA GTCCCTGGCA GAAGTGGTGT GCCTAGAGAT GGTGGACCTC 
CCCCTGACTG GGGCACAGGC CGAGCTGGAA GGAGAATTTG GCAAAAAGGC AGATGGCTTG 
CTGGGGATGT TCCTGAAACG CCTCTCGTCT CAGCTTATCC TGCTGCAAGC ATGGACTTCC 
CACCTCTGGA AAATGTTTTA TGATGCTCGG AAGCCCCGGA GTCAGATTAA GAATGAGATC 
AACATTGACA CCCTGGCCAG AGATGAATTC AACCTCCAGA AGATGATGGT GATGGTAACA 
GCCTCAGGCA AGCTTTTTGG CATTGAGAGC AGCTCTGGCA CCATCCTGTG GAAACAGTAT 
CTACCCAATG TCAAGCCAGA CTCCTCCTTT AAACTGATGG TCCAGAGAAC TACTGCTCAT 
TTCCCCCATC CCCCACAGTG CACCCTGCTG GTGAAGGACA AGGAGTCGGG AATGAGTTCT 
CTGTATGTCT TCAATCCCAT TTTTGGGAAG TGGAGTCAGG TAGCTCCCCC AGTGCTGAAG 
CGCCCCATCT TGCAGTCCTT GCTTCTCCCA GTCATGGATC AAGACTACGC CAAGGTGTTG 
CTGTTGATAG ATGATGAATA CAAGGTCACA GCTTTTCCAG CCACTCGGAA TGTCTTGCGA 
CAGCTACATG AGCTTGCCCC TTCCATCTTC TTCTATTTGG TGGATGCAGA GCAGGGACGG 
CTGTGTGGAT ATCGGCTTCG AAAGGATCTC ACCACTGAGC TGAGTTGGGA GCTGACCATT 
CCCCCAGAAG TACAGCGGAT CGTCAAGGTG AAGGGGAAAC GCAGCAGTGA GCACGTTCAT 
TCCCAGGGCC GTGTGATGGG GGACCGCAGT GTGCTCTACA AGAGCCTGAA CCCCAACCTG 
CTGGCCGTGG TGACAGAGAG CACAGACGCG CACCATGAGC GCACCTTTAT TGGCATCTTC 
CTCATTGATG GCGTCACTGG GCGTATCATT CACTCCTCTG TGCAGAAGAA AGCCAAAGGC 
CCTGTCCATA TCGTGCATTC AGAGAACTGG GTGGTGTACC AGTACTGGAA CACCAAGGCT 
CGGCGCAACG AGTTTACCGT ACTGGAGCTC TATGAGGGCA CTGAGCAATA CAACGCCACC 
GCCTTCAGCT CCCTGGACCG CCCCCAGCTG CCCCAGGTCC TCCAGCAGTC CTATATCTTC 
CCGTCCTCCA TCAGTGCCAT GGAGGCCACC ATCACCGAAC GGGGCATCAC CAGCCGACAC 
CTGCTGATTG GACTACCTTC TGGAGCAATT CTTTCCCTTC CTAAGGCTTT GCTGGATCCC 
CGCCGCCCCG AGATCCCAAC AGAACAAAGC AGAGAGGAGA ACTTAATCCC GTATTCTCCA 
GATGTACAGA TACACGCAGA GCGATTCATC AACTATAACC AGACAGTTTC TCGAATGCGA 
GGTATCTACA CAGCTCCCTC GGGTCTGGAG TCCACTTGTT TGGTTGTGGC CTATGGTTTG 
GACATTTACC AAACTCGAGT CTACCCATCC AAGCAGTTTG ACGTTCTGAA GGATGACTAT 
GACTACGTGT TAATCAGCAG CGTCCTCTTT GGCCTGGTTT TTGCCACCAT GATCACTAAG 
AGACTGGCAC AGGTGAAGCT CCTGAATCGG GCCTGGCGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055862.1
CDS	16..2997
Translation	MAAEWASRFWLWATLLIPAAAVYEDQVGKFDWRQQYVGKVKFASLEFSPGSKKLVVATEKNVIAALNSRTGEILWRHVDKGTAEGAVDAMLLHGQDVITVSNGGRIMRSWETNIGGLNWEITLDSGSFQALGLVGLQESVRYIAVLKKTTLALHHLSSGHLKWVEHLPESDSIHYQMVYSYGSGVVWALGVVPFSHVNIVKFNVEDGEIVQQVRVSTPWLQHLSGACGVVDEAVLVCPDPSSRSLQTLALETEWELRQIPLQSLDLEFGSGFQPRVLPTQPNPVDASRAQFFLHLSPSHYALLQYHYGTLSLLKNFPQTALVSFATTGEKTVAAVMACRNEVQKSSSSEDGSMGSFSEKSSSKDSLACFNQTYTINLYLVETGRRLLDTTITFSLEQSGTRPERLYIQVFLKKDDSVGYRALVQTEDHLLLFLQQLAGKVVLWSREESLAEVVCLEMVDLPLTGAQAELEGEFGKKADGLLGMFLKRLSSQLILLQAWTSHLWKMFYDARKPRSQIKNEINIDTLARDEFNLQKMMVMVTASGKLFGIESSSGTILWKQYLPNVKPDSSFKLMVQRTTAHFPHPPQCTLLVKDKESGMSSLYVFNPIFGKWSQVAPPVLKRPILQSLLLPVMDQDYAKVLLLIDDEYKVTAFPATRNVLRQLHELAPSIFFYLVDAEQGRLCGYRLRKDLTTELSWELTIPPEVQRIVKVKGKRSSEHVHSQGRVMGDRSVLYKSLNPNLLAVVTESTDAHHERTFIGIFLIDGVTGRIIHSSVQKKAKGPVHIVHSENWVVYQYWNTKARRNEFTVLELYEGTEQYNATAFSSLDRPQLPQVLQQSYIFPSSISAMEATITERGITSRHLLIGLPSGAILSLPKALLDPRRPEIPTEQSREENLIPYSPDVQIHAERFINYNQTVSRMRGIYTAPSGLESTCLVVAYGLDIYQTRVYPSKQFDVLKDDYDYVLISSVLFGLVFATMITKRLAQVKLLNRAWR

Target ORF information:

RefSeq Version	NM_015047.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015047.3

ORF Insert Sequence:

ATGGCGGCTG AGTGGGCTTC TCGTTTCTGG CTTTGGGCTA CGCTGCTGAT TCCTGCGGCC 
GCGGTCTACG AAGACCAAGT GGGCAAGTTT GATTGGAGAC AGCAATATGT TGGGAAGGTC 
AAGTTTGCCT CCTTGGAATT TTCCCCTGGA TCCAAGAAGT TGGTTGTAGC CACAGAGAAG 
AATGTGATTG CAGCATTAAA TTCCCGAACT GGGGAGATCT TGTGGCGCCA TGTTGACAAG 
GGCACGGCAG AAGGGGCTGT GGATGCCATG CTGCTGCACG GACAGGATGT GATCACTGTG 
TCCAATGGAG GCCGAATCAT GCGTTCCTGG GAGACTAACA TCGGGGGCCT GAACTGGGAG 
ATAACCCTGG ACAGTGGCAG TTTCCAGGCA CTTGGGCTGG TTGGCCTGCA GGAGTCTGTA 
AGGTACATCG CAGTCCTGAA GAAGACTACA CTTGCCCTCC ATCACCTCTC CAGTGGGCAC 
CTCAAGTGGG TGGAACATCT CCCAGAAAGT GACAGCATCC ACTACCAGAT GGTGTATTCT 
TACGGCTCTG GGGTGGTGTG GGCCCTCGGA GTTGTTCCCT TCAGCCATGT GAACATTGTC 
AAGTTTAATG TGGAAGATGG AGAGATTGTT CAGCAGGTTA GGGTTTCAAC TCCGTGGCTG 
CAGCACCTGT CTGGAGCCTG TGGTGTGGTG GATGAGGCTG TCCTGGTGTG TCCTGACCCG 
AGCTCACGTT CCCTCCAAAC TTTGGCTCTG GAGACGGAAT GGGAGTTGAG ACAGATCCCA 
CTGCAGTCTC TCGACTTAGA ATTTGGAAGT GGATTCCAAC CCCGGGTCCT GCCTACCCAG 
CCCAACCCAG TGGACGCTTC CCGGGCCCAG TTCTTCCTGC ACTTGTCCCC AAGCCACTAT 
GCTCTGCTGC AGTACCATTA TGGAACGCTG AGTTTGCTTA AAAACTTCCC ACAGACTGCC 
CTAGTGAGCT TTGCCACCAC TGGGGAGAAG ACGGTGGCTG CAGTCATGGC CTGTCGGAAT 
GAAGTGCAGA AAAGTAGCAG TTCTGAAGAT GGGTCAATGG GGAGCTTTTC GGAGAAGTCT 
AGTTCAAAGG ACTCTCTGGC TTGCTTCAAT CAGACCTACA CCATTAACCT ATACCTCGTG 
GAGACAGGTC GGCGGCTGCT GGACACCACG ATAACATTTA GCCTGGAACA GAGCGGCACT 
CGGCCTGAGC GGCTGTATAT CCAGGTGTTC TTGAAGAAGG ATGACTCAGT GGGCTACCGG 
GCTTTGGTGC AGACAGAGGA TCATCTGCTA CTTTTCCTGC AGCAGTTGGC AGGGAAGGTG 
GTGCTGTGGA GCCGTGAGGA GTCCCTGGCA GAAGTGGTGT GCCTAGAGAT GGTGGACCTC 
CCCCTGACTG GGGCACAGGC CGAGCTGGAA GGAGAATTTG GCAAAAAGGC AGATGGCTTG 
CTGGGGATGT TCCTGAAACG CCTCTCGTCT CAGCTTATCC TGCTGCAAGC ATGGACTTCC 
CACCTCTGGA AAATGTTTTA TGATGCTCGG AAGCCCCGGA GTCAGATTAA GAATGAGATC 
AACATTGACA CCCTGGCCAG AGATGAATTC AACCTCCAGA AGATGATGGT GATGGTAACA 
GCCTCAGGCA AGCTTTTTGG CATTGAGAGC AGCTCTGGCA CCATCCTGTG GAAACAGTAT 
CTACCCAATG TCAAGCCAGA CTCCTCCTTT AAACTGATGG TCCAGAGAAC TACTGCTCAT 
TTCCCCCATC CCCCACAGTG CACCCTGCTG GTGAAGGACA AGGAGTCGGG AATGAGTTCT 
CTGTATGTCT TCAATCCCAT TTTTGGGAAG TGGAGTCAGG TAGCTCCCCC AGTGCTGAAG 
CGCCCCATCT TGCAGTCCTT GCTTCTCCCA GTCATGGATC AAGACTACGC CAAGGTGTTG 
CTGTTGATAG ATGATGAATA CAAGGTCACA GCTTTTCCAG CCACTCGGAA TGTCTTGCGA 
CAGCTACATG AGCTTGCCCC TTCCATCTTC TTCTATTTGG TGGATGCAGA GCAGGGACGG 
CTGTGTGGAT ATCGGCTTCG AAAGGATCTC ACCACTGAGC TGAGTTGGGA GCTGACCATT 
CCCCCAGAAG TACAGCGGAT CGTCAAGGTG AAGGGGAAAC GCAGCAGTGA GCACGTTCAT 
TCCCAGGGCC GTGTGATGGG GGACCGCAGT GTGCTCTACA AGAGCCTGAA CCCCAACCTG 
CTGGCCGTGG TGACAGAGAG CACAGACGCG CACCATGAGC GCACCTTTAT TGGCATCTTC 
CTCATTGATG GCGTCACTGG GCGTATCATT CACTCCTCTG TGCAGAAGAA AGCCAAAGGC 
CCTGTCCATA TCGTGCATTC AGAGAACTGG GTGGTGTACC AGTACTGGAA CACCAAGGCT 
CGGCGCAACG AGTTTACCGT ACTGGAGCTC TATGAGGGCA CTGAGCAATA CAACGCCACC 
GCCTTCAGCT CCCTGGACCG CCCCCAGCTG CCCCAGGTCC TCCAGCAGTC CTATATCTTC 
CCGTCCTCCA TCAGTGCCAT GGAGGCCACC ATCACCGAAC GGGGCATCAC CAGCCGACAC 
CTGCTGATTG GACTACCTTC TGGAGCAATT CTTTCCCTTC CTAAGGCTTT GCTGGATCCC 
CGCCGCCCCG AGATCCCAAC AGAACAAAGC AGAGAGGAGA ACTTAATCCC GTATTCTCCA 
GATGTACAGA TACACGCAGA GCGATTCATC AACTATAACC AGACAGTTTC TCGAATGCGA 
GGTATCTACA CAGCTCCCTC GGGTCTGGAG TCCACTTGTT TGGTTGTGGC CTATGGTTTG 
GACATTTACC AAACTCGAGT CTACCCATCC AAGCAGTTTG ACGTTCTGAA GGATGACTAT 
GACTACGTGT TAATCAGCAG CGTCCTCTTT GGCCTGGTTT TTGCCACCAT GATCACTAAG 
AGACTGGCAC AGGTGAAGCT CCTGAATCGG GCCTGGCGAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMC1 ( NM_001271427.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258356.1 Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu10222
Clone ID Related Accession (Same CDS sequence)	NM_001271427.2 , NM_001271427.1
Accession Version	NM_001271427.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2979bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	ER membrane protein complex subunit 1 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB097686.1, D42044.2, AL357564.17 and BX648708.1. On May 31, 2019 this sequence version replaced NM_001271427.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. The resulting isoform (2) lacks an internal aa, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.157006.1, SRR1660805.258511.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGCTG AGTGGGCTTC TCGTTTCTGG CTTTGGGCTA CGCTGCTGAT TCCTGCGGCC 
GCGGTCTACG AAGACCAAGT GGGCAAGTTT GATTGGAGAC AGCAATATGT TGGGAAGGTC 
AAGTTTGCCT CCTTGGAATT TTCCCCTGGA TCCAAGAAGT TGGTTGTAGC CACAGAGAAG 
AATGTGATTG CAGCATTAAA TTCCCGAACT GGGGAGATCT TGTGGCGCCA TGTTGACAAG 
GGCACGGCAG AAGGGGCTGT GGATGCCATG CTGCTGCACG GACAGGATGT GATCACTGTG 
TCCAATGGAG GCCGAATCAT GCGTTCCTGG GAGACTAACA TCGGGGGCCT GAACTGGGAG 
ATAACCCTGG ACAGTGGCAG TTTCCAGGCA CTTGGGCTGG TTGGCCTGCA GGAGTCTGTA 
AGGTACATCG CAGTCCTGAA GAAGACTACA CTTGCCCTCC ATCACCTCTC CAGTGGGCAC 
CTCAAGTGGG TGGAACATCT CCCAGAAAGT GACAGCATCC ACTACCAGAT GGTGTATTCT 
TACGGCTCTG GGGTGGTGTG GGCCCTCGGA GTTGTTCCCT TCAGCCATGT GAACATTGTC 
AAGTTTAATG TGGAAGATGG AGAGATTGTT CAGCAGGTTA GGGTTTCAAC TCCGTGGCTG 
CAGCACCTGT CTGGAGCCTG TGGTGTGGTG GATGAGGCTG TCCTGGTGTG TCCTGACCCG 
AGCTCACGTT CCCTCCAAAC TTTGGCTCTG GAGACGGAAT GGGAGTTGAG ACAGATCCCA 
CTGCAGTCTC TCGACTTAGA ATTTGGAAGT GGATTCCAAC CCCGGGTCCT GCCTACCCAG 
CCCAACCCAG TGGACGCTTC CCGGGCCCAG TTCTTCCTGC ACTTGTCCCC AAGCCACTAT 
GCTCTGCTGC AGTACCATTA TGGAACGCTG AGTTTGCTTA AAAACTTCCC ACAGACTGCC 
CTAGTGAGCT TTGCCACCAC TGGGGAGAAG ACGGTGGCTG CAGTCATGGC CTGTCGGAAT 
GAAGTGAAAA GTAGCAGTTC TGAAGATGGG TCAATGGGGA GCTTTTCGGA GAAGTCTAGT 
TCAAAGGACT CTCTGGCTTG CTTCAATCAG ACCTACACCA TTAACCTATA CCTCGTGGAG 
ACAGGTCGGC GGCTGCTGGA CACCACGATA ACATTTAGCC TGGAACAGAG CGGCACTCGG 
CCTGAGCGGC TGTATATCCA GGTGTTCTTG AAGAAGGATG ACTCAGTGGG CTACCGGGCT 
TTGGTGCAGA CAGAGGATCA TCTGCTACTT TTCCTGCAGC AGTTGGCAGG GAAGGTGGTG 
CTGTGGAGCC GTGAGGAGTC CCTGGCAGAA GTGGTGTGCC TAGAGATGGT GGACCTCCCC 
CTGACTGGGG CACAGGCCGA GCTGGAAGGA GAATTTGGCA AAAAGGCAGA TGGCTTGCTG 
GGGATGTTCC TGAAACGCCT CTCGTCTCAG CTTATCCTGC TGCAAGCATG GACTTCCCAC 
CTCTGGAAAA TGTTTTATGA TGCTCGGAAG CCCCGGAGTC AGATTAAGAA TGAGATCAAC 
ATTGACACCC TGGCCAGAGA TGAATTCAAC CTCCAGAAGA TGATGGTGAT GGTAACAGCC 
TCAGGCAAGC TTTTTGGCAT TGAGAGCAGC TCTGGCACCA TCCTGTGGAA ACAGTATCTA 
CCCAATGTCA AGCCAGACTC CTCCTTTAAA CTGATGGTCC AGAGAACTAC TGCTCATTTC 
CCCCATCCCC CACAGTGCAC CCTGCTGGTG AAGGACAAGG AGTCGGGAAT GAGTTCTCTG 
TATGTCTTCA ATCCCATTTT TGGGAAGTGG AGTCAGGTAG CTCCCCCAGT GCTGAAGCGC 
CCCATCTTGC AGTCCTTGCT TCTCCCAGTC ATGGATCAAG ACTACGCCAA GGTGTTGCTG 
TTGATAGATG ATGAATACAA GGTCACAGCT TTTCCAGCCA CTCGGAATGT CTTGCGACAG 
CTACATGAGC TTGCCCCTTC CATCTTCTTC TATTTGGTGG ATGCAGAGCA GGGACGGCTG 
TGTGGATATC GGCTTCGAAA GGATCTCACC ACTGAGCTGA GTTGGGAGCT GACCATTCCC 
CCAGAAGTAC AGCGGATCGT CAAGGTGAAG GGGAAACGCA GCAGTGAGCA CGTTCATTCC 
CAGGGCCGTG TGATGGGGGA CCGCAGTGTG CTCTACAAGA GCCTGAACCC CAACCTGCTG 
GCCGTGGTGA CAGAGAGCAC AGACGCGCAC CATGAGCGCA CCTTTATTGG CATCTTCCTC 
ATTGATGGCG TCACTGGGCG TATCATTCAC TCCTCTGTGC AGAAGAAAGC CAAAGGCCCT 
GTCCATATCG TGCATTCAGA GAACTGGGTG GTGTACCAGT ACTGGAACAC CAAGGCTCGG 
CGCAACGAGT TTACCGTACT GGAGCTCTAT GAGGGCACTG AGCAATACAA CGCCACCGCC 
TTCAGCTCCC TGGACCGCCC CCAGCTGCCC CAGGTCCTCC AGCAGTCCTA TATCTTCCCG 
TCCTCCATCA GTGCCATGGA GGCCACCATC ACCGAACGGG GCATCACCAG CCGACACCTG 
CTGATTGGAC TACCTTCTGG AGCAATTCTT TCCCTTCCTA AGGCTTTGCT GGATCCCCGC 
CGCCCCGAGA TCCCAACAGA ACAAAGCAGA GAGGAGAACT TAATCCCGTA TTCTCCAGAT 
GTACAGATAC ACGCAGAGCG ATTCATCAAC TATAACCAGA CAGTTTCTCG AATGCGAGGT 
ATCTACACAG CTCCCTCGGG TCTGGAGTCC ACTTGTTTGG TTGTGGCCTA TGGTTTGGAC 
ATTTACCAAA CTCGAGTCTA CCCATCCAAG CAGTTTGACG TTCTGAAGGA TGACTATGAC 
TACGTGTTAA TCAGCAGCGT CCTCTTTGGC CTGGTTTTTG CCACCATGAT CACTAAGAGA 
CTGGCACAGG TGAAGCTCCT GAATCGGGCC TGGCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258356.1
CDS	16..2994
Translation	MAAEWASRFWLWATLLIPAAAVYEDQVGKFDWRQQYVGKVKFASLEFSPGSKKLVVATEKNVIAALNSRTGEILWRHVDKGTAEGAVDAMLLHGQDVITVSNGGRIMRSWETNIGGLNWEITLDSGSFQALGLVGLQESVRYIAVLKKTTLALHHLSSGHLKWVEHLPESDSIHYQMVYSYGSGVVWALGVVPFSHVNIVKFNVEDGEIVQQVRVSTPWLQHLSGACGVVDEAVLVCPDPSSRSLQTLALETEWELRQIPLQSLDLEFGSGFQPRVLPTQPNPVDASRAQFFLHLSPSHYALLQYHYGTLSLLKNFPQTALVSFATTGEKTVAAVMACRNEVKSSSSEDGSMGSFSEKSSSKDSLACFNQTYTINLYLVETGRRLLDTTITFSLEQSGTRPERLYIQVFLKKDDSVGYRALVQTEDHLLLFLQQLAGKVVLWSREESLAEVVCLEMVDLPLTGAQAELEGEFGKKADGLLGMFLKRLSSQLILLQAWTSHLWKMFYDARKPRSQIKNEINIDTLARDEFNLQKMMVMVTASGKLFGIESSSGTILWKQYLPNVKPDSSFKLMVQRTTAHFPHPPQCTLLVKDKESGMSSLYVFNPIFGKWSQVAPPVLKRPILQSLLLPVMDQDYAKVLLLIDDEYKVTAFPATRNVLRQLHELAPSIFFYLVDAEQGRLCGYRLRKDLTTELSWELTIPPEVQRIVKVKGKRSSEHVHSQGRVMGDRSVLYKSLNPNLLAVVTESTDAHHERTFIGIFLIDGVTGRIIHSSVQKKAKGPVHIVHSENWVVYQYWNTKARRNEFTVLELYEGTEQYNATAFSSLDRPQLPQVLQQSYIFPSSISAMEATITERGITSRHLLIGLPSGAILSLPKALLDPRRPEIPTEQSREENLIPYSPDVQIHAERFINYNQTVSRMRGIYTAPSGLESTCLVVAYGLDIYQTRVYPSKQFDVLKDDYDYVLISSVLFGLVFATMITKRLAQVKLLNRAWR

Target ORF information:

RefSeq Version	NM_001271427.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271427.2

ORF Insert Sequence:

ATGGCGGCTG AGTGGGCTTC TCGTTTCTGG CTTTGGGCTA CGCTGCTGAT TCCTGCGGCC 
GCGGTCTACG AAGACCAAGT GGGCAAGTTT GATTGGAGAC AGCAATATGT TGGGAAGGTC 
AAGTTTGCCT CCTTGGAATT TTCCCCTGGA TCCAAGAAGT TGGTTGTAGC CACAGAGAAG 
AATGTGATTG CAGCATTAAA TTCCCGAACT GGGGAGATCT TGTGGCGCCA TGTTGACAAG 
GGCACGGCAG AAGGGGCTGT GGATGCCATG CTGCTGCACG GACAGGATGT GATCACTGTG 
TCCAATGGAG GCCGAATCAT GCGTTCCTGG GAGACTAACA TCGGGGGCCT GAACTGGGAG 
ATAACCCTGG ACAGTGGCAG TTTCCAGGCA CTTGGGCTGG TTGGCCTGCA GGAGTCTGTA 
AGGTACATCG CAGTCCTGAA GAAGACTACA CTTGCCCTCC ATCACCTCTC CAGTGGGCAC 
CTCAAGTGGG TGGAACATCT CCCAGAAAGT GACAGCATCC ACTACCAGAT GGTGTATTCT 
TACGGCTCTG GGGTGGTGTG GGCCCTCGGA GTTGTTCCCT TCAGCCATGT GAACATTGTC 
AAGTTTAATG TGGAAGATGG AGAGATTGTT CAGCAGGTTA GGGTTTCAAC TCCGTGGCTG 
CAGCACCTGT CTGGAGCCTG TGGTGTGGTG GATGAGGCTG TCCTGGTGTG TCCTGACCCG 
AGCTCACGTT CCCTCCAAAC TTTGGCTCTG GAGACGGAAT GGGAGTTGAG ACAGATCCCA 
CTGCAGTCTC TCGACTTAGA ATTTGGAAGT GGATTCCAAC CCCGGGTCCT GCCTACCCAG 
CCCAACCCAG TGGACGCTTC CCGGGCCCAG TTCTTCCTGC ACTTGTCCCC AAGCCACTAT 
GCTCTGCTGC AGTACCATTA TGGAACGCTG AGTTTGCTTA AAAACTTCCC ACAGACTGCC 
CTAGTGAGCT TTGCCACCAC TGGGGAGAAG ACGGTGGCTG CAGTCATGGC CTGTCGGAAT 
GAAGTGAAAA GTAGCAGTTC TGAAGATGGG TCAATGGGGA GCTTTTCGGA GAAGTCTAGT 
TCAAAGGACT CTCTGGCTTG CTTCAATCAG ACCTACACCA TTAACCTATA CCTCGTGGAG 
ACAGGTCGGC GGCTGCTGGA CACCACGATA ACATTTAGCC TGGAACAGAG CGGCACTCGG 
CCTGAGCGGC TGTATATCCA GGTGTTCTTG AAGAAGGATG ACTCAGTGGG CTACCGGGCT 
TTGGTGCAGA CAGAGGATCA TCTGCTACTT TTCCTGCAGC AGTTGGCAGG GAAGGTGGTG 
CTGTGGAGCC GTGAGGAGTC CCTGGCAGAA GTGGTGTGCC TAGAGATGGT GGACCTCCCC 
CTGACTGGGG CACAGGCCGA GCTGGAAGGA GAATTTGGCA AAAAGGCAGA TGGCTTGCTG 
GGGATGTTCC TGAAACGCCT CTCGTCTCAG CTTATCCTGC TGCAAGCATG GACTTCCCAC 
CTCTGGAAAA TGTTTTATGA TGCTCGGAAG CCCCGGAGTC AGATTAAGAA TGAGATCAAC 
ATTGACACCC TGGCCAGAGA TGAATTCAAC CTCCAGAAGA TGATGGTGAT GGTAACAGCC 
TCAGGCAAGC TTTTTGGCAT TGAGAGCAGC TCTGGCACCA TCCTGTGGAA ACAGTATCTA 
CCCAATGTCA AGCCAGACTC CTCCTTTAAA CTGATGGTCC AGAGAACTAC TGCTCATTTC 
CCCCATCCCC CACAGTGCAC CCTGCTGGTG AAGGACAAGG AGTCGGGAAT GAGTTCTCTG 
TATGTCTTCA ATCCCATTTT TGGGAAGTGG AGTCAGGTAG CTCCCCCAGT GCTGAAGCGC 
CCCATCTTGC AGTCCTTGCT TCTCCCAGTC ATGGATCAAG ACTACGCCAA GGTGTTGCTG 
TTGATAGATG ATGAATACAA GGTCACAGCT TTTCCAGCCA CTCGGAATGT CTTGCGACAG 
CTACATGAGC TTGCCCCTTC CATCTTCTTC TATTTGGTGG ATGCAGAGCA GGGACGGCTG 
TGTGGATATC GGCTTCGAAA GGATCTCACC ACTGAGCTGA GTTGGGAGCT GACCATTCCC 
CCAGAAGTAC AGCGGATCGT CAAGGTGAAG GGGAAACGCA GCAGTGAGCA CGTTCATTCC 
CAGGGCCGTG TGATGGGGGA CCGCAGTGTG CTCTACAAGA GCCTGAACCC CAACCTGCTG 
GCCGTGGTGA CAGAGAGCAC AGACGCGCAC CATGAGCGCA CCTTTATTGG CATCTTCCTC 
ATTGATGGCG TCACTGGGCG TATCATTCAC TCCTCTGTGC AGAAGAAAGC CAAAGGCCCT 
GTCCATATCG TGCATTCAGA GAACTGGGTG GTGTACCAGT ACTGGAACAC CAAGGCTCGG 
CGCAACGAGT TTACCGTACT GGAGCTCTAT GAGGGCACTG AGCAATACAA CGCCACCGCC 
TTCAGCTCCC TGGACCGCCC CCAGCTGCCC CAGGTCCTCC AGCAGTCCTA TATCTTCCCG 
TCCTCCATCA GTGCCATGGA GGCCACCATC ACCGAACGGG GCATCACCAG CCGACACCTG 
CTGATTGGAC TACCTTCTGG AGCAATTCTT TCCCTTCCTA AGGCTTTGCT GGATCCCCGC 
CGCCCCGAGA TCCCAACAGA ACAAAGCAGA GAGGAGAACT TAATCCCGTA TTCTCCAGAT 
GTACAGATAC ACGCAGAGCG ATTCATCAAC TATAACCAGA CAGTTTCTCG AATGCGAGGT 
ATCTACACAG CTCCCTCGGG TCTGGAGTCC ACTTGTTTGG TTGTGGCCTA TGGTTTGGAC 
ATTTACCAAA CTCGAGTCTA CCCATCCAAG CAGTTTGACG TTCTGAAGGA TGACTATGAC 
TACGTGTTAA TCAGCAGCGT CCTCTTTGGC CTGGTTTTTG CCACCATGAT CACTAAGAGA 
CTGGCACAGG TGAAGCTCCT GAATCGGGCC TGGCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMC1 ( NM_001271427.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258356.1 Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu10222
Clone ID Related Accession (Same CDS sequence)	NM_001271427.2 , NM_001271427.1
Accession Version	NM_001271427.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2979bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	ER membrane protein complex subunit 1 isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB097686.1, D42044.2, AL357564.17 and BX648708.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. The resulting isoform (2) lacks an internal aa, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: D42044.2, SRR1660803.89995.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGCTG AGTGGGCTTC TCGTTTCTGG CTTTGGGCTA CGCTGCTGAT TCCTGCGGCC 
GCGGTCTACG AAGACCAAGT GGGCAAGTTT GATTGGAGAC AGCAATATGT TGGGAAGGTC 
AAGTTTGCCT CCTTGGAATT TTCCCCTGGA TCCAAGAAGT TGGTTGTAGC CACAGAGAAG 
AATGTGATTG CAGCATTAAA TTCCCGAACT GGGGAGATCT TGTGGCGCCA TGTTGACAAG 
GGCACGGCAG AAGGGGCTGT GGATGCCATG CTGCTGCACG GACAGGATGT GATCACTGTG 
TCCAATGGAG GCCGAATCAT GCGTTCCTGG GAGACTAACA TCGGGGGCCT GAACTGGGAG 
ATAACCCTGG ACAGTGGCAG TTTCCAGGCA CTTGGGCTGG TTGGCCTGCA GGAGTCTGTA 
AGGTACATCG CAGTCCTGAA GAAGACTACA CTTGCCCTCC ATCACCTCTC CAGTGGGCAC 
CTCAAGTGGG TGGAACATCT CCCAGAAAGT GACAGCATCC ACTACCAGAT GGTGTATTCT 
TACGGCTCTG GGGTGGTGTG GGCCCTCGGA GTTGTTCCCT TCAGCCATGT GAACATTGTC 
AAGTTTAATG TGGAAGATGG AGAGATTGTT CAGCAGGTTA GGGTTTCAAC TCCGTGGCTG 
CAGCACCTGT CTGGAGCCTG TGGTGTGGTG GATGAGGCTG TCCTGGTGTG TCCTGACCCG 
AGCTCACGTT CCCTCCAAAC TTTGGCTCTG GAGACGGAAT GGGAGTTGAG ACAGATCCCA 
CTGCAGTCTC TCGACTTAGA ATTTGGAAGT GGATTCCAAC CCCGGGTCCT GCCTACCCAG 
CCCAACCCAG TGGACGCTTC CCGGGCCCAG TTCTTCCTGC ACTTGTCCCC AAGCCACTAT 
GCTCTGCTGC AGTACCATTA TGGAACGCTG AGTTTGCTTA AAAACTTCCC ACAGACTGCC 
CTAGTGAGCT TTGCCACCAC TGGGGAGAAG ACGGTGGCTG CAGTCATGGC CTGTCGGAAT 
GAAGTGAAAA GTAGCAGTTC TGAAGATGGG TCAATGGGGA GCTTTTCGGA GAAGTCTAGT 
TCAAAGGACT CTCTGGCTTG CTTCAATCAG ACCTACACCA TTAACCTATA CCTCGTGGAG 
ACAGGTCGGC GGCTGCTGGA CACCACGATA ACATTTAGCC TGGAACAGAG CGGCACTCGG 
CCTGAGCGGC TGTATATCCA GGTGTTCTTG AAGAAGGATG ACTCAGTGGG CTACCGGGCT 
TTGGTGCAGA CAGAGGATCA TCTGCTACTT TTCCTGCAGC AGTTGGCAGG GAAGGTGGTG 
CTGTGGAGCC GTGAGGAGTC CCTGGCAGAA GTGGTGTGCC TAGAGATGGT GGACCTCCCC 
CTGACTGGGG CACAGGCCGA GCTGGAAGGA GAATTTGGCA AAAAGGCAGA TGGCTTGCTG 
GGGATGTTCC TGAAACGCCT CTCGTCTCAG CTTATCCTGC TGCAAGCATG GACTTCCCAC 
CTCTGGAAAA TGTTTTATGA TGCTCGGAAG CCCCGGAGTC AGATTAAGAA TGAGATCAAC 
ATTGACACCC TGGCCAGAGA TGAATTCAAC CTCCAGAAGA TGATGGTGAT GGTAACAGCC 
TCAGGCAAGC TTTTTGGCAT TGAGAGCAGC TCTGGCACCA TCCTGTGGAA ACAGTATCTA 
CCCAATGTCA AGCCAGACTC CTCCTTTAAA CTGATGGTCC AGAGAACTAC TGCTCATTTC 
CCCCATCCCC CACAGTGCAC CCTGCTGGTG AAGGACAAGG AGTCGGGAAT GAGTTCTCTG 
TATGTCTTCA ATCCCATTTT TGGGAAGTGG AGTCAGGTAG CTCCCCCAGT GCTGAAGCGC 
CCCATCTTGC AGTCCTTGCT TCTCCCAGTC ATGGATCAAG ACTACGCCAA GGTGTTGCTG 
TTGATAGATG ATGAATACAA GGTCACAGCT TTTCCAGCCA CTCGGAATGT CTTGCGACAG 
CTACATGAGC TTGCCCCTTC CATCTTCTTC TATTTGGTGG ATGCAGAGCA GGGACGGCTG 
TGTGGATATC GGCTTCGAAA GGATCTCACC ACTGAGCTGA GTTGGGAGCT GACCATTCCC 
CCAGAAGTAC AGCGGATCGT CAAGGTGAAG GGGAAACGCA GCAGTGAGCA CGTTCATTCC 
CAGGGCCGTG TGATGGGGGA CCGCAGTGTG CTCTACAAGA GCCTGAACCC CAACCTGCTG 
GCCGTGGTGA CAGAGAGCAC AGACGCGCAC CATGAGCGCA CCTTTATTGG CATCTTCCTC 
ATTGATGGCG TCACTGGGCG TATCATTCAC TCCTCTGTGC AGAAGAAAGC CAAAGGCCCT 
GTCCATATCG TGCATTCAGA GAACTGGGTG GTGTACCAGT ACTGGAACAC CAAGGCTCGG 
CGCAACGAGT TTACCGTACT GGAGCTCTAT GAGGGCACTG AGCAATACAA CGCCACCGCC 
TTCAGCTCCC TGGACCGCCC CCAGCTGCCC CAGGTCCTCC AGCAGTCCTA TATCTTCCCG 
TCCTCCATCA GTGCCATGGA GGCCACCATC ACCGAACGGG GCATCACCAG CCGACACCTG 
CTGATTGGAC TACCTTCTGG AGCAATTCTT TCCCTTCCTA AGGCTTTGCT GGATCCCCGC 
CGCCCCGAGA TCCCAACAGA ACAAAGCAGA GAGGAGAACT TAATCCCGTA TTCTCCAGAT 
GTACAGATAC ACGCAGAGCG ATTCATCAAC TATAACCAGA CAGTTTCTCG AATGCGAGGT 
ATCTACACAG CTCCCTCGGG TCTGGAGTCC ACTTGTTTGG TTGTGGCCTA TGGTTTGGAC 
ATTTACCAAA CTCGAGTCTA CCCATCCAAG CAGTTTGACG TTCTGAAGGA TGACTATGAC 
TACGTGTTAA TCAGCAGCGT CCTCTTTGGC CTGGTTTTTG CCACCATGAT CACTAAGAGA 
CTGGCACAGG TGAAGCTCCT GAATCGGGCC TGGCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258356.1
CDS	51..3029
Translation	MAAEWASRFWLWATLLIPAAAVYEDQVGKFDWRQQYVGKVKFASLEFSPGSKKLVVATEKNVIAALNSRTGEILWRHVDKGTAEGAVDAMLLHGQDVITVSNGGRIMRSWETNIGGLNWEITLDSGSFQALGLVGLQESVRYIAVLKKTTLALHHLSSGHLKWVEHLPESDSIHYQMVYSYGSGVVWALGVVPFSHVNIVKFNVEDGEIVQQVRVSTPWLQHLSGACGVVDEAVLVCPDPSSRSLQTLALETEWELRQIPLQSLDLEFGSGFQPRVLPTQPNPVDASRAQFFLHLSPSHYALLQYHYGTLSLLKNFPQTALVSFATTGEKTVAAVMACRNEVKSSSSEDGSMGSFSEKSSSKDSLACFNQTYTINLYLVETGRRLLDTTITFSLEQSGTRPERLYIQVFLKKDDSVGYRALVQTEDHLLLFLQQLAGKVVLWSREESLAEVVCLEMVDLPLTGAQAELEGEFGKKADGLLGMFLKRLSSQLILLQAWTSHLWKMFYDARKPRSQIKNEINIDTLARDEFNLQKMMVMVTASGKLFGIESSSGTILWKQYLPNVKPDSSFKLMVQRTTAHFPHPPQCTLLVKDKESGMSSLYVFNPIFGKWSQVAPPVLKRPILQSLLLPVMDQDYAKVLLLIDDEYKVTAFPATRNVLRQLHELAPSIFFYLVDAEQGRLCGYRLRKDLTTELSWELTIPPEVQRIVKVKGKRSSEHVHSQGRVMGDRSVLYKSLNPNLLAVVTESTDAHHERTFIGIFLIDGVTGRIIHSSVQKKAKGPVHIVHSENWVVYQYWNTKARRNEFTVLELYEGTEQYNATAFSSLDRPQLPQVLQQSYIFPSSISAMEATITERGITSRHLLIGLPSGAILSLPKALLDPRRPEIPTEQSREENLIPYSPDVQIHAERFINYNQTVSRMRGIYTAPSGLESTCLVVAYGLDIYQTRVYPSKQFDVLKDDYDYVLISSVLFGLVFATMITKRLAQVKLLNRAWR

Target ORF information:

RefSeq Version	NM_001271427.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271427.1

ORF Insert Sequence:

ATGGCGGCTG AGTGGGCTTC TCGTTTCTGG CTTTGGGCTA CGCTGCTGAT TCCTGCGGCC 
GCGGTCTACG AAGACCAAGT GGGCAAGTTT GATTGGAGAC AGCAATATGT TGGGAAGGTC 
AAGTTTGCCT CCTTGGAATT TTCCCCTGGA TCCAAGAAGT TGGTTGTAGC CACAGAGAAG 
AATGTGATTG CAGCATTAAA TTCCCGAACT GGGGAGATCT TGTGGCGCCA TGTTGACAAG 
GGCACGGCAG AAGGGGCTGT GGATGCCATG CTGCTGCACG GACAGGATGT GATCACTGTG 
TCCAATGGAG GCCGAATCAT GCGTTCCTGG GAGACTAACA TCGGGGGCCT GAACTGGGAG 
ATAACCCTGG ACAGTGGCAG TTTCCAGGCA CTTGGGCTGG TTGGCCTGCA GGAGTCTGTA 
AGGTACATCG CAGTCCTGAA GAAGACTACA CTTGCCCTCC ATCACCTCTC CAGTGGGCAC 
CTCAAGTGGG TGGAACATCT CCCAGAAAGT GACAGCATCC ACTACCAGAT GGTGTATTCT 
TACGGCTCTG GGGTGGTGTG GGCCCTCGGA GTTGTTCCCT TCAGCCATGT GAACATTGTC 
AAGTTTAATG TGGAAGATGG AGAGATTGTT CAGCAGGTTA GGGTTTCAAC TCCGTGGCTG 
CAGCACCTGT CTGGAGCCTG TGGTGTGGTG GATGAGGCTG TCCTGGTGTG TCCTGACCCG 
AGCTCACGTT CCCTCCAAAC TTTGGCTCTG GAGACGGAAT GGGAGTTGAG ACAGATCCCA 
CTGCAGTCTC TCGACTTAGA ATTTGGAAGT GGATTCCAAC CCCGGGTCCT GCCTACCCAG 
CCCAACCCAG TGGACGCTTC CCGGGCCCAG TTCTTCCTGC ACTTGTCCCC AAGCCACTAT 
GCTCTGCTGC AGTACCATTA TGGAACGCTG AGTTTGCTTA AAAACTTCCC ACAGACTGCC 
CTAGTGAGCT TTGCCACCAC TGGGGAGAAG ACGGTGGCTG CAGTCATGGC CTGTCGGAAT 
GAAGTGAAAA GTAGCAGTTC TGAAGATGGG TCAATGGGGA GCTTTTCGGA GAAGTCTAGT 
TCAAAGGACT CTCTGGCTTG CTTCAATCAG ACCTACACCA TTAACCTATA CCTCGTGGAG 
ACAGGTCGGC GGCTGCTGGA CACCACGATA ACATTTAGCC TGGAACAGAG CGGCACTCGG 
CCTGAGCGGC TGTATATCCA GGTGTTCTTG AAGAAGGATG ACTCAGTGGG CTACCGGGCT 
TTGGTGCAGA CAGAGGATCA TCTGCTACTT TTCCTGCAGC AGTTGGCAGG GAAGGTGGTG 
CTGTGGAGCC GTGAGGAGTC CCTGGCAGAA GTGGTGTGCC TAGAGATGGT GGACCTCCCC 
CTGACTGGGG CACAGGCCGA GCTGGAAGGA GAATTTGGCA AAAAGGCAGA TGGCTTGCTG 
GGGATGTTCC TGAAACGCCT CTCGTCTCAG CTTATCCTGC TGCAAGCATG GACTTCCCAC 
CTCTGGAAAA TGTTTTATGA TGCTCGGAAG CCCCGGAGTC AGATTAAGAA TGAGATCAAC 
ATTGACACCC TGGCCAGAGA TGAATTCAAC CTCCAGAAGA TGATGGTGAT GGTAACAGCC 
TCAGGCAAGC TTTTTGGCAT TGAGAGCAGC TCTGGCACCA TCCTGTGGAA ACAGTATCTA 
CCCAATGTCA AGCCAGACTC CTCCTTTAAA CTGATGGTCC AGAGAACTAC TGCTCATTTC 
CCCCATCCCC CACAGTGCAC CCTGCTGGTG AAGGACAAGG AGTCGGGAAT GAGTTCTCTG 
TATGTCTTCA ATCCCATTTT TGGGAAGTGG AGTCAGGTAG CTCCCCCAGT GCTGAAGCGC 
CCCATCTTGC AGTCCTTGCT TCTCCCAGTC ATGGATCAAG ACTACGCCAA GGTGTTGCTG 
TTGATAGATG ATGAATACAA GGTCACAGCT TTTCCAGCCA CTCGGAATGT CTTGCGACAG 
CTACATGAGC TTGCCCCTTC CATCTTCTTC TATTTGGTGG ATGCAGAGCA GGGACGGCTG 
TGTGGATATC GGCTTCGAAA GGATCTCACC ACTGAGCTGA GTTGGGAGCT GACCATTCCC 
CCAGAAGTAC AGCGGATCGT CAAGGTGAAG GGGAAACGCA GCAGTGAGCA CGTTCATTCC 
CAGGGCCGTG TGATGGGGGA CCGCAGTGTG CTCTACAAGA GCCTGAACCC CAACCTGCTG 
GCCGTGGTGA CAGAGAGCAC AGACGCGCAC CATGAGCGCA CCTTTATTGG CATCTTCCTC 
ATTGATGGCG TCACTGGGCG TATCATTCAC TCCTCTGTGC AGAAGAAAGC CAAAGGCCCT 
GTCCATATCG TGCATTCAGA GAACTGGGTG GTGTACCAGT ACTGGAACAC CAAGGCTCGG 
CGCAACGAGT TTACCGTACT GGAGCTCTAT GAGGGCACTG AGCAATACAA CGCCACCGCC 
TTCAGCTCCC TGGACCGCCC CCAGCTGCCC CAGGTCCTCC AGCAGTCCTA TATCTTCCCG 
TCCTCCATCA GTGCCATGGA GGCCACCATC ACCGAACGGG GCATCACCAG CCGACACCTG 
CTGATTGGAC TACCTTCTGG AGCAATTCTT TCCCTTCCTA AGGCTTTGCT GGATCCCCGC 
CGCCCCGAGA TCCCAACAGA ACAAAGCAGA GAGGAGAACT TAATCCCGTA TTCTCCAGAT 
GTACAGATAC ACGCAGAGCG ATTCATCAAC TATAACCAGA CAGTTTCTCG AATGCGAGGT 
ATCTACACAG CTCCCTCGGG TCTGGAGTCC ACTTGTTTGG TTGTGGCCTA TGGTTTGGAC 
ATTTACCAAA CTCGAGTCTA CCCATCCAAG CAGTTTGACG TTCTGAAGGA TGACTATGAC 
TACGTGTTAA TCAGCAGCGT CCTCTTTGGC CTGGTTTTTG CCACCATGAT CACTAAGAGA 
CTGGCACAGG TGAAGCTCCT GAATCGGGCC TGGCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMC1 ( NM_001271428.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258357.1 Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu11738
Clone ID Related Accession (Same CDS sequence)	NM_001271428.2 , NM_001271428.1
Accession Version	NM_001271428.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2979bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	ER membrane protein complex subunit 1 isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB097686.1, BX648708.1, BC034589.1 and AL357564.17. On May 31, 2019 this sequence version replaced NM_001271428.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the central region, compared to variant 1. The resulting isoform (2) lacks an internal aa, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BX648708.1, SRR1803617.70306.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGCTG AGTGGGCTTC TCGTTTCTGG CTTTGGGCTA CGCTGCTGAT TCCTGCGGCC 
GCGGTCTACG AAGACCAAGT GGGCAAGTTT GATTGGAGAC AGCAATATGT TGGGAAGGTC 
AAGTTTGCCT CCTTGGAATT TTCCCCTGGA TCCAAGAAGT TGGTTGTAGC CACAGAGAAG 
AATGTGATTG CAGCATTAAA TTCCCGAACT GGGGAGATCT TGTGGCGCCA TGTTGACAAG 
GGCACGGCAG AAGGGGCTGT GGATGCCATG CTGCTGCACG GACAGGATGT GATCACTGTG 
TCCAATGGAG GCCGAATCAT GCGTTCCTGG GAGACTAACA TCGGGGGCCT GAACTGGGAG 
ATAACCCTGG ACAGTGGCAG TTTCCAGGCA CTTGGGCTGG TTGGCCTGCA GGAGTCTGTA 
AGGTACATCG CAGTCCTGAA GAAGACTACA CTTGCCCTCC ATCACCTCTC CAGTGGGCAC 
CTCAAGTGGG TGGAACATCT CCCAGAAAGT GACAGCATCC ACTACCAGAT GGTGTATTCT 
TACGGCTCTG GGGTGGTGTG GGCCCTCGGA GTTGTTCCCT TCAGCCATGT GAACATTGTC 
AAGTTTAATG TGGAAGATGG AGAGATTGTT CAGCAGGTTA GGGTTTCAAC TCCGTGGCTG 
CAGCACCTGT CTGGAGCCTG TGGTGTGGTG GATGAGGCTG TCCTGGTGTG TCCTGACCCG 
AGCTCACGTT CCCTCCAAAC TTTGGCTCTG GAGACGGAAT GGGAGTTGAG ACAGATCCCA 
CTGCAGTCTC TCGACTTAGA ATTTGGAAGT GGATTCCAAC CCCGGGTCCT GCCTACCCAG 
CCCAACCCAG TGGACGCTTC CCGGGCCCAG TTCTTCCTGC ACTTGTCCCC AAGCCACTAT 
GCTCTGCTGC AGTACCATTA TGGAACGCTG AGTTTGCTTA AAAACTTCCC ACAGACTGCC 
CTAGTGAGCT TTGCCACCAC TGGGGAGAAG ACGGTGGCTG CAGTCATGGC CTGTCGGAAT 
GAAGTGCAGA AAAGTAGCAG TTCTGAAGAT GGGTCAATGG GGAGCTTTTC GGAGAAGTCT 
AGTTCAAAGG ACTCTCTGGC TTGCTTCAAT CAGACCTACA CCATTAACCT ATACCTCGTG 
GAGACAGGTC GGCGGCTGCT GGACACCACG ATAACATTTA GCCTGGAACA GAGCGGCACT 
CGGCCTGAGC GGCTGTATAT CCAGGTGTTC TTGAAGAAGG ATGACTCAGT GGGCTACCGG 
GCTTTGGTGC AGACAGAGGA TCATCTGCTA CTTTTCCTGC AGCAGTTGGG GAAGGTGGTG 
CTGTGGAGCC GTGAGGAGTC CCTGGCAGAA GTGGTGTGCC TAGAGATGGT GGACCTCCCC 
CTGACTGGGG CACAGGCCGA GCTGGAAGGA GAATTTGGCA AAAAGGCAGA TGGCTTGCTG 
GGGATGTTCC TGAAACGCCT CTCGTCTCAG CTTATCCTGC TGCAAGCATG GACTTCCCAC 
CTCTGGAAAA TGTTTTATGA TGCTCGGAAG CCCCGGAGTC AGATTAAGAA TGAGATCAAC 
ATTGACACCC TGGCCAGAGA TGAATTCAAC CTCCAGAAGA TGATGGTGAT GGTAACAGCC 
TCAGGCAAGC TTTTTGGCAT TGAGAGCAGC TCTGGCACCA TCCTGTGGAA ACAGTATCTA 
CCCAATGTCA AGCCAGACTC CTCCTTTAAA CTGATGGTCC AGAGAACTAC TGCTCATTTC 
CCCCATCCCC CACAGTGCAC CCTGCTGGTG AAGGACAAGG AGTCGGGAAT GAGTTCTCTG 
TATGTCTTCA ATCCCATTTT TGGGAAGTGG AGTCAGGTAG CTCCCCCAGT GCTGAAGCGC 
CCCATCTTGC AGTCCTTGCT TCTCCCAGTC ATGGATCAAG ACTACGCCAA GGTGTTGCTG 
TTGATAGATG ATGAATACAA GGTCACAGCT TTTCCAGCCA CTCGGAATGT CTTGCGACAG 
CTACATGAGC TTGCCCCTTC CATCTTCTTC TATTTGGTGG ATGCAGAGCA GGGACGGCTG 
TGTGGATATC GGCTTCGAAA GGATCTCACC ACTGAGCTGA GTTGGGAGCT GACCATTCCC 
CCAGAAGTAC AGCGGATCGT CAAGGTGAAG GGGAAACGCA GCAGTGAGCA CGTTCATTCC 
CAGGGCCGTG TGATGGGGGA CCGCAGTGTG CTCTACAAGA GCCTGAACCC CAACCTGCTG 
GCCGTGGTGA CAGAGAGCAC AGACGCGCAC CATGAGCGCA CCTTTATTGG CATCTTCCTC 
ATTGATGGCG TCACTGGGCG TATCATTCAC TCCTCTGTGC AGAAGAAAGC CAAAGGCCCT 
GTCCATATCG TGCATTCAGA GAACTGGGTG GTGTACCAGT ACTGGAACAC CAAGGCTCGG 
CGCAACGAGT TTACCGTACT GGAGCTCTAT GAGGGCACTG AGCAATACAA CGCCACCGCC 
TTCAGCTCCC TGGACCGCCC CCAGCTGCCC CAGGTCCTCC AGCAGTCCTA TATCTTCCCG 
TCCTCCATCA GTGCCATGGA GGCCACCATC ACCGAACGGG GCATCACCAG CCGACACCTG 
CTGATTGGAC TACCTTCTGG AGCAATTCTT TCCCTTCCTA AGGCTTTGCT GGATCCCCGC 
CGCCCCGAGA TCCCAACAGA ACAAAGCAGA GAGGAGAACT TAATCCCGTA TTCTCCAGAT 
GTACAGATAC ACGCAGAGCG ATTCATCAAC TATAACCAGA CAGTTTCTCG AATGCGAGGT 
ATCTACACAG CTCCCTCGGG TCTGGAGTCC ACTTGTTTGG TTGTGGCCTA TGGTTTGGAC 
ATTTACCAAA CTCGAGTCTA CCCATCCAAG CAGTTTGACG TTCTGAAGGA TGACTATGAC 
TACGTGTTAA TCAGCAGCGT CCTCTTTGGC CTGGTTTTTG CCACCATGAT CACTAAGAGA 
CTGGCACAGG TGAAGCTCCT GAATCGGGCC TGGCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258357.1
CDS	16..2994
Translation	MAAEWASRFWLWATLLIPAAAVYEDQVGKFDWRQQYVGKVKFASLEFSPGSKKLVVATEKNVIAALNSRTGEILWRHVDKGTAEGAVDAMLLHGQDVITVSNGGRIMRSWETNIGGLNWEITLDSGSFQALGLVGLQESVRYIAVLKKTTLALHHLSSGHLKWVEHLPESDSIHYQMVYSYGSGVVWALGVVPFSHVNIVKFNVEDGEIVQQVRVSTPWLQHLSGACGVVDEAVLVCPDPSSRSLQTLALETEWELRQIPLQSLDLEFGSGFQPRVLPTQPNPVDASRAQFFLHLSPSHYALLQYHYGTLSLLKNFPQTALVSFATTGEKTVAAVMACRNEVQKSSSSEDGSMGSFSEKSSSKDSLACFNQTYTINLYLVETGRRLLDTTITFSLEQSGTRPERLYIQVFLKKDDSVGYRALVQTEDHLLLFLQQLGKVVLWSREESLAEVVCLEMVDLPLTGAQAELEGEFGKKADGLLGMFLKRLSSQLILLQAWTSHLWKMFYDARKPRSQIKNEINIDTLARDEFNLQKMMVMVTASGKLFGIESSSGTILWKQYLPNVKPDSSFKLMVQRTTAHFPHPPQCTLLVKDKESGMSSLYVFNPIFGKWSQVAPPVLKRPILQSLLLPVMDQDYAKVLLLIDDEYKVTAFPATRNVLRQLHELAPSIFFYLVDAEQGRLCGYRLRKDLTTELSWELTIPPEVQRIVKVKGKRSSEHVHSQGRVMGDRSVLYKSLNPNLLAVVTESTDAHHERTFIGIFLIDGVTGRIIHSSVQKKAKGPVHIVHSENWVVYQYWNTKARRNEFTVLELYEGTEQYNATAFSSLDRPQLPQVLQQSYIFPSSISAMEATITERGITSRHLLIGLPSGAILSLPKALLDPRRPEIPTEQSREENLIPYSPDVQIHAERFINYNQTVSRMRGIYTAPSGLESTCLVVAYGLDIYQTRVYPSKQFDVLKDDYDYVLISSVLFGLVFATMITKRLAQVKLLNRAWR

Target ORF information:

RefSeq Version	NM_001271428.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271428.2

ORF Insert Sequence:

ATGGCGGCTG AGTGGGCTTC TCGTTTCTGG CTTTGGGCTA CGCTGCTGAT TCCTGCGGCC 
GCGGTCTACG AAGACCAAGT GGGCAAGTTT GATTGGAGAC AGCAATATGT TGGGAAGGTC 
AAGTTTGCCT CCTTGGAATT TTCCCCTGGA TCCAAGAAGT TGGTTGTAGC CACAGAGAAG 
AATGTGATTG CAGCATTAAA TTCCCGAACT GGGGAGATCT TGTGGCGCCA TGTTGACAAG 
GGCACGGCAG AAGGGGCTGT GGATGCCATG CTGCTGCACG GACAGGATGT GATCACTGTG 
TCCAATGGAG GCCGAATCAT GCGTTCCTGG GAGACTAACA TCGGGGGCCT GAACTGGGAG 
ATAACCCTGG ACAGTGGCAG TTTCCAGGCA CTTGGGCTGG TTGGCCTGCA GGAGTCTGTA 
AGGTACATCG CAGTCCTGAA GAAGACTACA CTTGCCCTCC ATCACCTCTC CAGTGGGCAC 
CTCAAGTGGG TGGAACATCT CCCAGAAAGT GACAGCATCC ACTACCAGAT GGTGTATTCT 
TACGGCTCTG GGGTGGTGTG GGCCCTCGGA GTTGTTCCCT TCAGCCATGT GAACATTGTC 
AAGTTTAATG TGGAAGATGG AGAGATTGTT CAGCAGGTTA GGGTTTCAAC TCCGTGGCTG 
CAGCACCTGT CTGGAGCCTG TGGTGTGGTG GATGAGGCTG TCCTGGTGTG TCCTGACCCG 
AGCTCACGTT CCCTCCAAAC TTTGGCTCTG GAGACGGAAT GGGAGTTGAG ACAGATCCCA 
CTGCAGTCTC TCGACTTAGA ATTTGGAAGT GGATTCCAAC CCCGGGTCCT GCCTACCCAG 
CCCAACCCAG TGGACGCTTC CCGGGCCCAG TTCTTCCTGC ACTTGTCCCC AAGCCACTAT 
GCTCTGCTGC AGTACCATTA TGGAACGCTG AGTTTGCTTA AAAACTTCCC ACAGACTGCC 
CTAGTGAGCT TTGCCACCAC TGGGGAGAAG ACGGTGGCTG CAGTCATGGC CTGTCGGAAT 
GAAGTGCAGA AAAGTAGCAG TTCTGAAGAT GGGTCAATGG GGAGCTTTTC GGAGAAGTCT 
AGTTCAAAGG ACTCTCTGGC TTGCTTCAAT CAGACCTACA CCATTAACCT ATACCTCGTG 
GAGACAGGTC GGCGGCTGCT GGACACCACG ATAACATTTA GCCTGGAACA GAGCGGCACT 
CGGCCTGAGC GGCTGTATAT CCAGGTGTTC TTGAAGAAGG ATGACTCAGT GGGCTACCGG 
GCTTTGGTGC AGACAGAGGA TCATCTGCTA CTTTTCCTGC AGCAGTTGGG GAAGGTGGTG 
CTGTGGAGCC GTGAGGAGTC CCTGGCAGAA GTGGTGTGCC TAGAGATGGT GGACCTCCCC 
CTGACTGGGG CACAGGCCGA GCTGGAAGGA GAATTTGGCA AAAAGGCAGA TGGCTTGCTG 
GGGATGTTCC TGAAACGCCT CTCGTCTCAG CTTATCCTGC TGCAAGCATG GACTTCCCAC 
CTCTGGAAAA TGTTTTATGA TGCTCGGAAG CCCCGGAGTC AGATTAAGAA TGAGATCAAC 
ATTGACACCC TGGCCAGAGA TGAATTCAAC CTCCAGAAGA TGATGGTGAT GGTAACAGCC 
TCAGGCAAGC TTTTTGGCAT TGAGAGCAGC TCTGGCACCA TCCTGTGGAA ACAGTATCTA 
CCCAATGTCA AGCCAGACTC CTCCTTTAAA CTGATGGTCC AGAGAACTAC TGCTCATTTC 
CCCCATCCCC CACAGTGCAC CCTGCTGGTG AAGGACAAGG AGTCGGGAAT GAGTTCTCTG 
TATGTCTTCA ATCCCATTTT TGGGAAGTGG AGTCAGGTAG CTCCCCCAGT GCTGAAGCGC 
CCCATCTTGC AGTCCTTGCT TCTCCCAGTC ATGGATCAAG ACTACGCCAA GGTGTTGCTG 
TTGATAGATG ATGAATACAA GGTCACAGCT TTTCCAGCCA CTCGGAATGT CTTGCGACAG 
CTACATGAGC TTGCCCCTTC CATCTTCTTC TATTTGGTGG ATGCAGAGCA GGGACGGCTG 
TGTGGATATC GGCTTCGAAA GGATCTCACC ACTGAGCTGA GTTGGGAGCT GACCATTCCC 
CCAGAAGTAC AGCGGATCGT CAAGGTGAAG GGGAAACGCA GCAGTGAGCA CGTTCATTCC 
CAGGGCCGTG TGATGGGGGA CCGCAGTGTG CTCTACAAGA GCCTGAACCC CAACCTGCTG 
GCCGTGGTGA CAGAGAGCAC AGACGCGCAC CATGAGCGCA CCTTTATTGG CATCTTCCTC 
ATTGATGGCG TCACTGGGCG TATCATTCAC TCCTCTGTGC AGAAGAAAGC CAAAGGCCCT 
GTCCATATCG TGCATTCAGA GAACTGGGTG GTGTACCAGT ACTGGAACAC CAAGGCTCGG 
CGCAACGAGT TTACCGTACT GGAGCTCTAT GAGGGCACTG AGCAATACAA CGCCACCGCC 
TTCAGCTCCC TGGACCGCCC CCAGCTGCCC CAGGTCCTCC AGCAGTCCTA TATCTTCCCG 
TCCTCCATCA GTGCCATGGA GGCCACCATC ACCGAACGGG GCATCACCAG CCGACACCTG 
CTGATTGGAC TACCTTCTGG AGCAATTCTT TCCCTTCCTA AGGCTTTGCT GGATCCCCGC 
CGCCCCGAGA TCCCAACAGA ACAAAGCAGA GAGGAGAACT TAATCCCGTA TTCTCCAGAT 
GTACAGATAC ACGCAGAGCG ATTCATCAAC TATAACCAGA CAGTTTCTCG AATGCGAGGT 
ATCTACACAG CTCCCTCGGG TCTGGAGTCC ACTTGTTTGG TTGTGGCCTA TGGTTTGGAC 
ATTTACCAAA CTCGAGTCTA CCCATCCAAG CAGTTTGACG TTCTGAAGGA TGACTATGAC 
TACGTGTTAA TCAGCAGCGT CCTCTTTGGC CTGGTTTTTG CCACCATGAT CACTAAGAGA 
CTGGCACAGG TGAAGCTCCT GAATCGGGCC TGGCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMC1 ( NM_001271428.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258357.1 Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu11738
Clone ID Related Accession (Same CDS sequence)	NM_001271428.2 , NM_001271428.1
Accession Version	NM_001271428.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2979bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	ER membrane protein complex subunit 1 isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB097686.1, BX648708.1, BC034589.1 and AL357564.17. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the central region, compared to variant 1. The resulting isoform (2) lacks an internal aa, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BX648708.1, SRR1803617.70306.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGCTG AGTGGGCTTC TCGTTTCTGG CTTTGGGCTA CGCTGCTGAT TCCTGCGGCC 
GCGGTCTACG AAGACCAAGT GGGCAAGTTT GATTGGAGAC AGCAATATGT TGGGAAGGTC 
AAGTTTGCCT CCTTGGAATT TTCCCCTGGA TCCAAGAAGT TGGTTGTAGC CACAGAGAAG 
AATGTGATTG CAGCATTAAA TTCCCGAACT GGGGAGATCT TGTGGCGCCA TGTTGACAAG 
GGCACGGCAG AAGGGGCTGT GGATGCCATG CTGCTGCACG GACAGGATGT GATCACTGTG 
TCCAATGGAG GCCGAATCAT GCGTTCCTGG GAGACTAACA TCGGGGGCCT GAACTGGGAG 
ATAACCCTGG ACAGTGGCAG TTTCCAGGCA CTTGGGCTGG TTGGCCTGCA GGAGTCTGTA 
AGGTACATCG CAGTCCTGAA GAAGACTACA CTTGCCCTCC ATCACCTCTC CAGTGGGCAC 
CTCAAGTGGG TGGAACATCT CCCAGAAAGT GACAGCATCC ACTACCAGAT GGTGTATTCT 
TACGGCTCTG GGGTGGTGTG GGCCCTCGGA GTTGTTCCCT TCAGCCATGT GAACATTGTC 
AAGTTTAATG TGGAAGATGG AGAGATTGTT CAGCAGGTTA GGGTTTCAAC TCCGTGGCTG 
CAGCACCTGT CTGGAGCCTG TGGTGTGGTG GATGAGGCTG TCCTGGTGTG TCCTGACCCG 
AGCTCACGTT CCCTCCAAAC TTTGGCTCTG GAGACGGAAT GGGAGTTGAG ACAGATCCCA 
CTGCAGTCTC TCGACTTAGA ATTTGGAAGT GGATTCCAAC CCCGGGTCCT GCCTACCCAG 
CCCAACCCAG TGGACGCTTC CCGGGCCCAG TTCTTCCTGC ACTTGTCCCC AAGCCACTAT 
GCTCTGCTGC AGTACCATTA TGGAACGCTG AGTTTGCTTA AAAACTTCCC ACAGACTGCC 
CTAGTGAGCT TTGCCACCAC TGGGGAGAAG ACGGTGGCTG CAGTCATGGC CTGTCGGAAT 
GAAGTGCAGA AAAGTAGCAG TTCTGAAGAT GGGTCAATGG GGAGCTTTTC GGAGAAGTCT 
AGTTCAAAGG ACTCTCTGGC TTGCTTCAAT CAGACCTACA CCATTAACCT ATACCTCGTG 
GAGACAGGTC GGCGGCTGCT GGACACCACG ATAACATTTA GCCTGGAACA GAGCGGCACT 
CGGCCTGAGC GGCTGTATAT CCAGGTGTTC TTGAAGAAGG ATGACTCAGT GGGCTACCGG 
GCTTTGGTGC AGACAGAGGA TCATCTGCTA CTTTTCCTGC AGCAGTTGGG GAAGGTGGTG 
CTGTGGAGCC GTGAGGAGTC CCTGGCAGAA GTGGTGTGCC TAGAGATGGT GGACCTCCCC 
CTGACTGGGG CACAGGCCGA GCTGGAAGGA GAATTTGGCA AAAAGGCAGA TGGCTTGCTG 
GGGATGTTCC TGAAACGCCT CTCGTCTCAG CTTATCCTGC TGCAAGCATG GACTTCCCAC 
CTCTGGAAAA TGTTTTATGA TGCTCGGAAG CCCCGGAGTC AGATTAAGAA TGAGATCAAC 
ATTGACACCC TGGCCAGAGA TGAATTCAAC CTCCAGAAGA TGATGGTGAT GGTAACAGCC 
TCAGGCAAGC TTTTTGGCAT TGAGAGCAGC TCTGGCACCA TCCTGTGGAA ACAGTATCTA 
CCCAATGTCA AGCCAGACTC CTCCTTTAAA CTGATGGTCC AGAGAACTAC TGCTCATTTC 
CCCCATCCCC CACAGTGCAC CCTGCTGGTG AAGGACAAGG AGTCGGGAAT GAGTTCTCTG 
TATGTCTTCA ATCCCATTTT TGGGAAGTGG AGTCAGGTAG CTCCCCCAGT GCTGAAGCGC 
CCCATCTTGC AGTCCTTGCT TCTCCCAGTC ATGGATCAAG ACTACGCCAA GGTGTTGCTG 
TTGATAGATG ATGAATACAA GGTCACAGCT TTTCCAGCCA CTCGGAATGT CTTGCGACAG 
CTACATGAGC TTGCCCCTTC CATCTTCTTC TATTTGGTGG ATGCAGAGCA GGGACGGCTG 
TGTGGATATC GGCTTCGAAA GGATCTCACC ACTGAGCTGA GTTGGGAGCT GACCATTCCC 
CCAGAAGTAC AGCGGATCGT CAAGGTGAAG GGGAAACGCA GCAGTGAGCA CGTTCATTCC 
CAGGGCCGTG TGATGGGGGA CCGCAGTGTG CTCTACAAGA GCCTGAACCC CAACCTGCTG 
GCCGTGGTGA CAGAGAGCAC AGACGCGCAC CATGAGCGCA CCTTTATTGG CATCTTCCTC 
ATTGATGGCG TCACTGGGCG TATCATTCAC TCCTCTGTGC AGAAGAAAGC CAAAGGCCCT 
GTCCATATCG TGCATTCAGA GAACTGGGTG GTGTACCAGT ACTGGAACAC CAAGGCTCGG 
CGCAACGAGT TTACCGTACT GGAGCTCTAT GAGGGCACTG AGCAATACAA CGCCACCGCC 
TTCAGCTCCC TGGACCGCCC CCAGCTGCCC CAGGTCCTCC AGCAGTCCTA TATCTTCCCG 
TCCTCCATCA GTGCCATGGA GGCCACCATC ACCGAACGGG GCATCACCAG CCGACACCTG 
CTGATTGGAC TACCTTCTGG AGCAATTCTT TCCCTTCCTA AGGCTTTGCT GGATCCCCGC 
CGCCCCGAGA TCCCAACAGA ACAAAGCAGA GAGGAGAACT TAATCCCGTA TTCTCCAGAT 
GTACAGATAC ACGCAGAGCG ATTCATCAAC TATAACCAGA CAGTTTCTCG AATGCGAGGT 
ATCTACACAG CTCCCTCGGG TCTGGAGTCC ACTTGTTTGG TTGTGGCCTA TGGTTTGGAC 
ATTTACCAAA CTCGAGTCTA CCCATCCAAG CAGTTTGACG TTCTGAAGGA TGACTATGAC 
TACGTGTTAA TCAGCAGCGT CCTCTTTGGC CTGGTTTTTG CCACCATGAT CACTAAGAGA 
CTGGCACAGG TGAAGCTCCT GAATCGGGCC TGGCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258357.1
CDS	51..3029
Translation	MAAEWASRFWLWATLLIPAAAVYEDQVGKFDWRQQYVGKVKFASLEFSPGSKKLVVATEKNVIAALNSRTGEILWRHVDKGTAEGAVDAMLLHGQDVITVSNGGRIMRSWETNIGGLNWEITLDSGSFQALGLVGLQESVRYIAVLKKTTLALHHLSSGHLKWVEHLPESDSIHYQMVYSYGSGVVWALGVVPFSHVNIVKFNVEDGEIVQQVRVSTPWLQHLSGACGVVDEAVLVCPDPSSRSLQTLALETEWELRQIPLQSLDLEFGSGFQPRVLPTQPNPVDASRAQFFLHLSPSHYALLQYHYGTLSLLKNFPQTALVSFATTGEKTVAAVMACRNEVQKSSSSEDGSMGSFSEKSSSKDSLACFNQTYTINLYLVETGRRLLDTTITFSLEQSGTRPERLYIQVFLKKDDSVGYRALVQTEDHLLLFLQQLGKVVLWSREESLAEVVCLEMVDLPLTGAQAELEGEFGKKADGLLGMFLKRLSSQLILLQAWTSHLWKMFYDARKPRSQIKNEINIDTLARDEFNLQKMMVMVTASGKLFGIESSSGTILWKQYLPNVKPDSSFKLMVQRTTAHFPHPPQCTLLVKDKESGMSSLYVFNPIFGKWSQVAPPVLKRPILQSLLLPVMDQDYAKVLLLIDDEYKVTAFPATRNVLRQLHELAPSIFFYLVDAEQGRLCGYRLRKDLTTELSWELTIPPEVQRIVKVKGKRSSEHVHSQGRVMGDRSVLYKSLNPNLLAVVTESTDAHHERTFIGIFLIDGVTGRIIHSSVQKKAKGPVHIVHSENWVVYQYWNTKARRNEFTVLELYEGTEQYNATAFSSLDRPQLPQVLQQSYIFPSSISAMEATITERGITSRHLLIGLPSGAILSLPKALLDPRRPEIPTEQSREENLIPYSPDVQIHAERFINYNQTVSRMRGIYTAPSGLESTCLVVAYGLDIYQTRVYPSKQFDVLKDDYDYVLISSVLFGLVFATMITKRLAQVKLLNRAWR

Target ORF information:

RefSeq Version	NM_001271428.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271428.1

ORF Insert Sequence:

ATGGCGGCTG AGTGGGCTTC TCGTTTCTGG CTTTGGGCTA CGCTGCTGAT TCCTGCGGCC 
GCGGTCTACG AAGACCAAGT GGGCAAGTTT GATTGGAGAC AGCAATATGT TGGGAAGGTC 
AAGTTTGCCT CCTTGGAATT TTCCCCTGGA TCCAAGAAGT TGGTTGTAGC CACAGAGAAG 
AATGTGATTG CAGCATTAAA TTCCCGAACT GGGGAGATCT TGTGGCGCCA TGTTGACAAG 
GGCACGGCAG AAGGGGCTGT GGATGCCATG CTGCTGCACG GACAGGATGT GATCACTGTG 
TCCAATGGAG GCCGAATCAT GCGTTCCTGG GAGACTAACA TCGGGGGCCT GAACTGGGAG 
ATAACCCTGG ACAGTGGCAG TTTCCAGGCA CTTGGGCTGG TTGGCCTGCA GGAGTCTGTA 
AGGTACATCG CAGTCCTGAA GAAGACTACA CTTGCCCTCC ATCACCTCTC CAGTGGGCAC 
CTCAAGTGGG TGGAACATCT CCCAGAAAGT GACAGCATCC ACTACCAGAT GGTGTATTCT 
TACGGCTCTG GGGTGGTGTG GGCCCTCGGA GTTGTTCCCT TCAGCCATGT GAACATTGTC 
AAGTTTAATG TGGAAGATGG AGAGATTGTT CAGCAGGTTA GGGTTTCAAC TCCGTGGCTG 
CAGCACCTGT CTGGAGCCTG TGGTGTGGTG GATGAGGCTG TCCTGGTGTG TCCTGACCCG 
AGCTCACGTT CCCTCCAAAC TTTGGCTCTG GAGACGGAAT GGGAGTTGAG ACAGATCCCA 
CTGCAGTCTC TCGACTTAGA ATTTGGAAGT GGATTCCAAC CCCGGGTCCT GCCTACCCAG 
CCCAACCCAG TGGACGCTTC CCGGGCCCAG TTCTTCCTGC ACTTGTCCCC AAGCCACTAT 
GCTCTGCTGC AGTACCATTA TGGAACGCTG AGTTTGCTTA AAAACTTCCC ACAGACTGCC 
CTAGTGAGCT TTGCCACCAC TGGGGAGAAG ACGGTGGCTG CAGTCATGGC CTGTCGGAAT 
GAAGTGCAGA AAAGTAGCAG TTCTGAAGAT GGGTCAATGG GGAGCTTTTC GGAGAAGTCT 
AGTTCAAAGG ACTCTCTGGC TTGCTTCAAT CAGACCTACA CCATTAACCT ATACCTCGTG 
GAGACAGGTC GGCGGCTGCT GGACACCACG ATAACATTTA GCCTGGAACA GAGCGGCACT 
CGGCCTGAGC GGCTGTATAT CCAGGTGTTC TTGAAGAAGG ATGACTCAGT GGGCTACCGG 
GCTTTGGTGC AGACAGAGGA TCATCTGCTA CTTTTCCTGC AGCAGTTGGG GAAGGTGGTG 
CTGTGGAGCC GTGAGGAGTC CCTGGCAGAA GTGGTGTGCC TAGAGATGGT GGACCTCCCC 
CTGACTGGGG CACAGGCCGA GCTGGAAGGA GAATTTGGCA AAAAGGCAGA TGGCTTGCTG 
GGGATGTTCC TGAAACGCCT CTCGTCTCAG CTTATCCTGC TGCAAGCATG GACTTCCCAC 
CTCTGGAAAA TGTTTTATGA TGCTCGGAAG CCCCGGAGTC AGATTAAGAA TGAGATCAAC 
ATTGACACCC TGGCCAGAGA TGAATTCAAC CTCCAGAAGA TGATGGTGAT GGTAACAGCC 
TCAGGCAAGC TTTTTGGCAT TGAGAGCAGC TCTGGCACCA TCCTGTGGAA ACAGTATCTA 
CCCAATGTCA AGCCAGACTC CTCCTTTAAA CTGATGGTCC AGAGAACTAC TGCTCATTTC 
CCCCATCCCC CACAGTGCAC CCTGCTGGTG AAGGACAAGG AGTCGGGAAT GAGTTCTCTG 
TATGTCTTCA ATCCCATTTT TGGGAAGTGG AGTCAGGTAG CTCCCCCAGT GCTGAAGCGC 
CCCATCTTGC AGTCCTTGCT TCTCCCAGTC ATGGATCAAG ACTACGCCAA GGTGTTGCTG 
TTGATAGATG ATGAATACAA GGTCACAGCT TTTCCAGCCA CTCGGAATGT CTTGCGACAG 
CTACATGAGC TTGCCCCTTC CATCTTCTTC TATTTGGTGG ATGCAGAGCA GGGACGGCTG 
TGTGGATATC GGCTTCGAAA GGATCTCACC ACTGAGCTGA GTTGGGAGCT GACCATTCCC 
CCAGAAGTAC AGCGGATCGT CAAGGTGAAG GGGAAACGCA GCAGTGAGCA CGTTCATTCC 
CAGGGCCGTG TGATGGGGGA CCGCAGTGTG CTCTACAAGA GCCTGAACCC CAACCTGCTG 
GCCGTGGTGA CAGAGAGCAC AGACGCGCAC CATGAGCGCA CCTTTATTGG CATCTTCCTC 
ATTGATGGCG TCACTGGGCG TATCATTCAC TCCTCTGTGC AGAAGAAAGC CAAAGGCCCT 
GTCCATATCG TGCATTCAGA GAACTGGGTG GTGTACCAGT ACTGGAACAC CAAGGCTCGG 
CGCAACGAGT TTACCGTACT GGAGCTCTAT GAGGGCACTG AGCAATACAA CGCCACCGCC 
TTCAGCTCCC TGGACCGCCC CCAGCTGCCC CAGGTCCTCC AGCAGTCCTA TATCTTCCCG 
TCCTCCATCA GTGCCATGGA GGCCACCATC ACCGAACGGG GCATCACCAG CCGACACCTG 
CTGATTGGAC TACCTTCTGG AGCAATTCTT TCCCTTCCTA AGGCTTTGCT GGATCCCCGC 
CGCCCCGAGA TCCCAACAGA ACAAAGCAGA GAGGAGAACT TAATCCCGTA TTCTCCAGAT 
GTACAGATAC ACGCAGAGCG ATTCATCAAC TATAACCAGA CAGTTTCTCG AATGCGAGGT 
ATCTACACAG CTCCCTCGGG TCTGGAGTCC ACTTGTTTGG TTGTGGCCTA TGGTTTGGAC 
ATTTACCAAA CTCGAGTCTA CCCATCCAAG CAGTTTGACG TTCTGAAGGA TGACTATGAC 
TACGTGTTAA TCAGCAGCGT CCTCTTTGGC CTGGTTTTTG CCACCATGAT CACTAAGAGA 
CTGGCACAGG TGAAGCTCCT GAATCGGGCC TGGCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMC1 ( NM_001271429.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258358.1 Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant 4, mRNA.

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CloneID	OHu12432
Clone ID Related Accession (Same CDS sequence)	NM_001271429.1 , NM_001271429.2
Accession Version	NM_001271429.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-07
Organism	Homo sapiens(human)
Product	ER membrane protein complex subunit 1 isoform 4 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB097686.1, AK075563.1, BC034589.1, AL357564.17 and BX648708.1. Transcript Variant: This variant (4) lacks an in-frame exon in the 5' coding region, compared to variant 1. The resulting isoform (4) lacks an internal segment, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK075563.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGCTG AGTGGGCTTC TCGTTTCTGG CTTTGGGCTA CGCTGCTGAT TCCTGCGGCC 
GCGGTCTACG AAGACCAAGT GGGCAAGTTT GATTGGAGAC AGCAATATGT TGGGAAGGTC 
AAGTTTGCCT CCTTGGAATT TTCCCCTGGA TCCAAGAAGT TGGTTGTAGC CACAGAGAAG 
AATGTGATTG CAGCATTAAA TTCCCGAACT GGGGAGATCT ATGTGATCAC TGTGTCCAAT 
GGAGGCCGAA TCATGCGTTC CTGGGAGACT AACATCGGGG GCCTGAACTG GGAGATAACC 
CTGGACAGTG GCAGTTTCCA GGCACTTGGG CTGGTTGGCC TGCAGGAGTC TGTAAGGTAC 
ATCGCAGTCC TGAAGAAGAC TACACTTGCC CTCCATCACC TCTCCAGTGG GCACCTCAAG 
TGGGTGGAAC ATCTCCCAGA AAGTGACAGC ATCCACTACC AGATGGTGTA TTCTTACGGC 
TCTGGGGTGG TGTGGGCCCT CGGAGTTGTT CCCTTCAGCC ATGTGAACAT TGTCAAGTTT 
AATGTGGAAG ATGGAGAGAT TGTTCAGCAG GTTAGGGTTT CAACTCCGTG GCTGCAGCAC 
CTGTCTGGAG CCTGTGGTGT GGTGGATGAG GCTGTCCTGG TGTGTCCTGA CCCGAGCTCA 
CGTTCCCTCC AAACTTTGGC TCTGGAGACG GAATGGGAGT TGAGACAGAT CCCACTGCAG 
TCTCTCGACT TAGAATTTGG AAGTGGATTC CAACCCCGGG TCCTGCCTAC CCAGCCCAAC 
CCAGTGGACG CTTCCCGGGC CCAGTTCTTC CTGCACTTGT CCCCAAGCCA CTATGCTCTG 
CTGCAGTACC ATTATGGAAC GCTGAGTTTG CTTAAAAACT TCCCACAGAC TGCCCTAGTG 
AGCTTTGCCA CCACTGGGGA GAAGACGGTG GCTGCAGTCA TGGCCTGTCG GAATGAAGTG 
CAGAAAAGTA GCAGTTCTGA AGATGGGTCA ATGGGGAGCT TTTCGGAGAA GTCTAGTTCA 
AAGGACTCTC TGGCTTGCTT CAATCAGACC TACACCATTA ACCTATACCT CGTGGAGACA 
GGTCGGCGGC TGCTGGACAC CACGATAACA TTTAGCCTGG AACAGAGCGG CACTCGGCCT 
GAGCGGCTGT ATATCCAGGT GTTCTTGAAG AAGGATGACT CAGTGGGCTA CCGGGCTTTG 
GTGCAGACAG AGGATCATCT GCTACTTTTC CTGCAGCAGT TGGCAGGGAA GGTGGTGCTG 
TGGAGCCGTG AGGAGTCCCT GGCAGAAGTG GTGTGCCTAG AGATGGTGGA CCTCCCCCTG 
ACTGGGGCAC AGGCCGAGCT GGAAGGAGAA TTTGGCAAAA AGGCAGATGG CTTGCTGGGG 
ATGTTCCTGA AACGCCTCTC GTCTCAGCTT ATCCTGCTGC AAGCATGGAC TTCCCACCTC 
TGGAAAATGT TTTATGATGC TCGGAAGCCC CGGAGTCAGA TTAAGAATGA GATCAACATT 
GACACCCTGG CCAGAGATGA ATTCAACCTC CAGAAGATGA TGGTGATGGT AACAGCCTCA 
GGCAAGCTTT TTGGCATTGA GAGCAGCTCT GGCACCATCC TGTGGAAACA GTATCTACCC 
AATGTCAAGC CAGACTCCTC CTTTAAACTG ATGGTCCAGA GAACTACTGC TCATTTCCCC 
CATCCCCCAC AGTGCACCCT GCTGGTGAAG GACAAGGAGT CGGGAATGAG TTCTCTGTAT 
GTCTTCAATC CCATTTTTGG GAAGTGGAGT CAGGTAGCTC CCCCAGTGCT GAAGCGCCCC 
ATCTTGCAGT CCTTGCTTCT CCCAGTCATG GATCAAGACT ACGCCAAGGT GTTGCTGTTG 
ATAGATGATG AATACAAGGT CACAGCTTTT CCAGCCACTC GGAATGTCTT GCGACAGCTA 
CATGAGCTTG CCCCTTCCAT CTTCTTCTAT TTGGTGGATG CAGAGCAGGG ACGGCTGTGT 
GGATATCGGC TTCGAAAGGA TCTCACCACT GAGCTGAGTT GGGAGCTGAC CATTCCCCCA 
GAAGTACAGC GGATCGTCAA GGTGAAGGGG AAACGCAGCA GTGAGCACGT TCATTCCCAG 
GGCCGTGTGA TGGGGGACCG CAGTGTGCTC TACAAGAGCC TGAACCCCAA CCTGCTGGCC 
GTGGTGACAG AGAGCACAGA CGCGCACCAT GAGCGCACCT TTATTGGCAT CTTCCTCATT 
GATGGCGTCA CTGGGCGTAT CATTCACTCC TCTGTGCAGA AGAAAGCCAA AGGCCCTGTC 
CATATCGTGC ATTCAGAGAA CTGGGTGGTG TACCAGTACT GGAACACCAA GGCTCGGCGC 
AACGAGTTTA CCGTACTGGA GCTCTATGAG GGCACTGAGC AATACAACGC CACCGCCTTC 
AGCTCCCTGG ACCGCCCCCA GCTGCCCCAG GTCCTCCAGC AGTCCTATAT CTTCCCGTCC 
TCCATCAGTG CCATGGAGGC CACCATCACC GAACGGGGCA TCACCAGCCG ACACCTGCTG 
ATTGGACTAC CTTCTGGAGC AATTCTTTCC CTTCCTAAGG CTTTGCTGGA TCCCCGCCGC 
CCCGAGATCC CAACAGAACA AAGCAGAGAG GAGAACTTAA TCCCGTATTC TCCAGATGTA 
CAGATACACG CAGAGCGATT CATCAACTAT AACCAGACAG TTTCTCGAAT GCGAGGTATC 
TACACAGCTC CCTCGGGTCT GGAGTCCACT TGTTTGGTTG TGGCCTATGG TTTGGACATT 
TACCAAACTC GAGTCTACCC ATCCAAGCAG TTTGACGTTC TGAAGGATGA CTATGACTAC 
GTGTTAATCA GCAGCGTCCT CTTTGGCCTG GTTTTTGCCA CCATGATCAC TAAGAGACTG 
GCACAGGTGA AGCTCCTGAA TCGGGCCTGG CGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258358.1
CDS	51..2966
Translation	MAAEWASRFWLWATLLIPAAAVYEDQVGKFDWRQQYVGKVKFASLEFSPGSKKLVVATEKNVIAALNSRTGEIYVITVSNGGRIMRSWETNIGGLNWEITLDSGSFQALGLVGLQESVRYIAVLKKTTLALHHLSSGHLKWVEHLPESDSIHYQMVYSYGSGVVWALGVVPFSHVNIVKFNVEDGEIVQQVRVSTPWLQHLSGACGVVDEAVLVCPDPSSRSLQTLALETEWELRQIPLQSLDLEFGSGFQPRVLPTQPNPVDASRAQFFLHLSPSHYALLQYHYGTLSLLKNFPQTALVSFATTGEKTVAAVMACRNEVQKSSSSEDGSMGSFSEKSSSKDSLACFNQTYTINLYLVETGRRLLDTTITFSLEQSGTRPERLYIQVFLKKDDSVGYRALVQTEDHLLLFLQQLAGKVVLWSREESLAEVVCLEMVDLPLTGAQAELEGEFGKKADGLLGMFLKRLSSQLILLQAWTSHLWKMFYDARKPRSQIKNEINIDTLARDEFNLQKMMVMVTASGKLFGIESSSGTILWKQYLPNVKPDSSFKLMVQRTTAHFPHPPQCTLLVKDKESGMSSLYVFNPIFGKWSQVAPPVLKRPILQSLLLPVMDQDYAKVLLLIDDEYKVTAFPATRNVLRQLHELAPSIFFYLVDAEQGRLCGYRLRKDLTTELSWELTIPPEVQRIVKVKGKRSSEHVHSQGRVMGDRSVLYKSLNPNLLAVVTESTDAHHERTFIGIFLIDGVTGRIIHSSVQKKAKGPVHIVHSENWVVYQYWNTKARRNEFTVLELYEGTEQYNATAFSSLDRPQLPQVLQQSYIFPSSISAMEATITERGITSRHLLIGLPSGAILSLPKALLDPRRPEIPTEQSREENLIPYSPDVQIHAERFINYNQTVSRMRGIYTAPSGLESTCLVVAYGLDIYQTRVYPSKQFDVLKDDYDYVLISSVLFGLVFATMITKRLAQVKLLNRAWR

Target ORF information:

RefSeq Version	NM_001271429.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271429.1

ORF Insert Sequence:

ATGGCGGCTG AGTGGGCTTC TCGTTTCTGG CTTTGGGCTA CGCTGCTGAT TCCTGCGGCC 
GCGGTCTACG AAGACCAAGT GGGCAAGTTT GATTGGAGAC AGCAATATGT TGGGAAGGTC 
AAGTTTGCCT CCTTGGAATT TTCCCCTGGA TCCAAGAAGT TGGTTGTAGC CACAGAGAAG 
AATGTGATTG CAGCATTAAA TTCCCGAACT GGGGAGATCT ATGTGATCAC TGTGTCCAAT 
GGAGGCCGAA TCATGCGTTC CTGGGAGACT AACATCGGGG GCCTGAACTG GGAGATAACC 
CTGGACAGTG GCAGTTTCCA GGCACTTGGG CTGGTTGGCC TGCAGGAGTC TGTAAGGTAC 
ATCGCAGTCC TGAAGAAGAC TACACTTGCC CTCCATCACC TCTCCAGTGG GCACCTCAAG 
TGGGTGGAAC ATCTCCCAGA AAGTGACAGC ATCCACTACC AGATGGTGTA TTCTTACGGC 
TCTGGGGTGG TGTGGGCCCT CGGAGTTGTT CCCTTCAGCC ATGTGAACAT TGTCAAGTTT 
AATGTGGAAG ATGGAGAGAT TGTTCAGCAG GTTAGGGTTT CAACTCCGTG GCTGCAGCAC 
CTGTCTGGAG CCTGTGGTGT GGTGGATGAG GCTGTCCTGG TGTGTCCTGA CCCGAGCTCA 
CGTTCCCTCC AAACTTTGGC TCTGGAGACG GAATGGGAGT TGAGACAGAT CCCACTGCAG 
TCTCTCGACT TAGAATTTGG AAGTGGATTC CAACCCCGGG TCCTGCCTAC CCAGCCCAAC 
CCAGTGGACG CTTCCCGGGC CCAGTTCTTC CTGCACTTGT CCCCAAGCCA CTATGCTCTG 
CTGCAGTACC ATTATGGAAC GCTGAGTTTG CTTAAAAACT TCCCACAGAC TGCCCTAGTG 
AGCTTTGCCA CCACTGGGGA GAAGACGGTG GCTGCAGTCA TGGCCTGTCG GAATGAAGTG 
CAGAAAAGTA GCAGTTCTGA AGATGGGTCA ATGGGGAGCT TTTCGGAGAA GTCTAGTTCA 
AAGGACTCTC TGGCTTGCTT CAATCAGACC TACACCATTA ACCTATACCT CGTGGAGACA 
GGTCGGCGGC TGCTGGACAC CACGATAACA TTTAGCCTGG AACAGAGCGG CACTCGGCCT 
GAGCGGCTGT ATATCCAGGT GTTCTTGAAG AAGGATGACT CAGTGGGCTA CCGGGCTTTG 
GTGCAGACAG AGGATCATCT GCTACTTTTC CTGCAGCAGT TGGCAGGGAA GGTGGTGCTG 
TGGAGCCGTG AGGAGTCCCT GGCAGAAGTG GTGTGCCTAG AGATGGTGGA CCTCCCCCTG 
ACTGGGGCAC AGGCCGAGCT GGAAGGAGAA TTTGGCAAAA AGGCAGATGG CTTGCTGGGG 
ATGTTCCTGA AACGCCTCTC GTCTCAGCTT ATCCTGCTGC AAGCATGGAC TTCCCACCTC 
TGGAAAATGT TTTATGATGC TCGGAAGCCC CGGAGTCAGA TTAAGAATGA GATCAACATT 
GACACCCTGG CCAGAGATGA ATTCAACCTC CAGAAGATGA TGGTGATGGT AACAGCCTCA 
GGCAAGCTTT TTGGCATTGA GAGCAGCTCT GGCACCATCC TGTGGAAACA GTATCTACCC 
AATGTCAAGC CAGACTCCTC CTTTAAACTG ATGGTCCAGA GAACTACTGC TCATTTCCCC 
CATCCCCCAC AGTGCACCCT GCTGGTGAAG GACAAGGAGT CGGGAATGAG TTCTCTGTAT 
GTCTTCAATC CCATTTTTGG GAAGTGGAGT CAGGTAGCTC CCCCAGTGCT GAAGCGCCCC 
ATCTTGCAGT CCTTGCTTCT CCCAGTCATG GATCAAGACT ACGCCAAGGT GTTGCTGTTG 
ATAGATGATG AATACAAGGT CACAGCTTTT CCAGCCACTC GGAATGTCTT GCGACAGCTA 
CATGAGCTTG CCCCTTCCAT CTTCTTCTAT TTGGTGGATG CAGAGCAGGG ACGGCTGTGT 
GGATATCGGC TTCGAAAGGA TCTCACCACT GAGCTGAGTT GGGAGCTGAC CATTCCCCCA 
GAAGTACAGC GGATCGTCAA GGTGAAGGGG AAACGCAGCA GTGAGCACGT TCATTCCCAG 
GGCCGTGTGA TGGGGGACCG CAGTGTGCTC TACAAGAGCC TGAACCCCAA CCTGCTGGCC 
GTGGTGACAG AGAGCACAGA CGCGCACCAT GAGCGCACCT TTATTGGCAT CTTCCTCATT 
GATGGCGTCA CTGGGCGTAT CATTCACTCC TCTGTGCAGA AGAAAGCCAA AGGCCCTGTC 
CATATCGTGC ATTCAGAGAA CTGGGTGGTG TACCAGTACT GGAACACCAA GGCTCGGCGC 
AACGAGTTTA CCGTACTGGA GCTCTATGAG GGCACTGAGC AATACAACGC CACCGCCTTC 
AGCTCCCTGG ACCGCCCCCA GCTGCCCCAG GTCCTCCAGC AGTCCTATAT CTTCCCGTCC 
TCCATCAGTG CCATGGAGGC CACCATCACC GAACGGGGCA TCACCAGCCG ACACCTGCTG 
ATTGGACTAC CTTCTGGAGC AATTCTTTCC CTTCCTAAGG CTTTGCTGGA TCCCCGCCGC 
CCCGAGATCC CAACAGAACA AAGCAGAGAG GAGAACTTAA TCCCGTATTC TCCAGATGTA 
CAGATACACG CAGAGCGATT CATCAACTAT AACCAGACAG TTTCTCGAAT GCGAGGTATC 
TACACAGCTC CCTCGGGTCT GGAGTCCACT TGTTTGGTTG TGGCCTATGG TTTGGACATT 
TACCAAACTC GAGTCTACCC ATCCAAGCAG TTTGACGTTC TGAAGGATGA CTATGACTAC 
GTGTTAATCA GCAGCGTCCT CTTTGGCCTG GTTTTTGCCA CCATGATCAC TAAGAGACTG 
GCACAGGTGA AGCTCCTGAA TCGGGCCTGG CGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMC1 ( NM_001271429.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258358.1 Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu12432
Clone ID Related Accession (Same CDS sequence)	NM_001271429.1 , NM_001271429.2
Accession Version	NM_001271429.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	ER membrane protein complex subunit 1 isoform 4 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB097686.1, AK075563.1, BC034589.1, AL357564.17 and BX648708.1. On May 31, 2019 this sequence version replaced NM_001271429.1. Transcript Variant: This variant (4) lacks an in-frame exon in the 5' coding region, compared to variant 1. The resulting isoform (4) lacks an internal segment, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK075563.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGCTG AGTGGGCTTC TCGTTTCTGG CTTTGGGCTA CGCTGCTGAT TCCTGCGGCC 
GCGGTCTACG AAGACCAAGT GGGCAAGTTT GATTGGAGAC AGCAATATGT TGGGAAGGTC 
AAGTTTGCCT CCTTGGAATT TTCCCCTGGA TCCAAGAAGT TGGTTGTAGC CACAGAGAAG 
AATGTGATTG CAGCATTAAA TTCCCGAACT GGGGAGATCT ATGTGATCAC TGTGTCCAAT 
GGAGGCCGAA TCATGCGTTC CTGGGAGACT AACATCGGGG GCCTGAACTG GGAGATAACC 
CTGGACAGTG GCAGTTTCCA GGCACTTGGG CTGGTTGGCC TGCAGGAGTC TGTAAGGTAC 
ATCGCAGTCC TGAAGAAGAC TACACTTGCC CTCCATCACC TCTCCAGTGG GCACCTCAAG 
TGGGTGGAAC ATCTCCCAGA AAGTGACAGC ATCCACTACC AGATGGTGTA TTCTTACGGC 
TCTGGGGTGG TGTGGGCCCT CGGAGTTGTT CCCTTCAGCC ATGTGAACAT TGTCAAGTTT 
AATGTGGAAG ATGGAGAGAT TGTTCAGCAG GTTAGGGTTT CAACTCCGTG GCTGCAGCAC 
CTGTCTGGAG CCTGTGGTGT GGTGGATGAG GCTGTCCTGG TGTGTCCTGA CCCGAGCTCA 
CGTTCCCTCC AAACTTTGGC TCTGGAGACG GAATGGGAGT TGAGACAGAT CCCACTGCAG 
TCTCTCGACT TAGAATTTGG AAGTGGATTC CAACCCCGGG TCCTGCCTAC CCAGCCCAAC 
CCAGTGGACG CTTCCCGGGC CCAGTTCTTC CTGCACTTGT CCCCAAGCCA CTATGCTCTG 
CTGCAGTACC ATTATGGAAC GCTGAGTTTG CTTAAAAACT TCCCACAGAC TGCCCTAGTG 
AGCTTTGCCA CCACTGGGGA GAAGACGGTG GCTGCAGTCA TGGCCTGTCG GAATGAAGTG 
CAGAAAAGTA GCAGTTCTGA AGATGGGTCA ATGGGGAGCT TTTCGGAGAA GTCTAGTTCA 
AAGGACTCTC TGGCTTGCTT CAATCAGACC TACACCATTA ACCTATACCT CGTGGAGACA 
GGTCGGCGGC TGCTGGACAC CACGATAACA TTTAGCCTGG AACAGAGCGG CACTCGGCCT 
GAGCGGCTGT ATATCCAGGT GTTCTTGAAG AAGGATGACT CAGTGGGCTA CCGGGCTTTG 
GTGCAGACAG AGGATCATCT GCTACTTTTC CTGCAGCAGT TGGCAGGGAA GGTGGTGCTG 
TGGAGCCGTG AGGAGTCCCT GGCAGAAGTG GTGTGCCTAG AGATGGTGGA CCTCCCCCTG 
ACTGGGGCAC AGGCCGAGCT GGAAGGAGAA TTTGGCAAAA AGGCAGATGG CTTGCTGGGG 
ATGTTCCTGA AACGCCTCTC GTCTCAGCTT ATCCTGCTGC AAGCATGGAC TTCCCACCTC 
TGGAAAATGT TTTATGATGC TCGGAAGCCC CGGAGTCAGA TTAAGAATGA GATCAACATT 
GACACCCTGG CCAGAGATGA ATTCAACCTC CAGAAGATGA TGGTGATGGT AACAGCCTCA 
GGCAAGCTTT TTGGCATTGA GAGCAGCTCT GGCACCATCC TGTGGAAACA GTATCTACCC 
AATGTCAAGC CAGACTCCTC CTTTAAACTG ATGGTCCAGA GAACTACTGC TCATTTCCCC 
CATCCCCCAC AGTGCACCCT GCTGGTGAAG GACAAGGAGT CGGGAATGAG TTCTCTGTAT 
GTCTTCAATC CCATTTTTGG GAAGTGGAGT CAGGTAGCTC CCCCAGTGCT GAAGCGCCCC 
ATCTTGCAGT CCTTGCTTCT CCCAGTCATG GATCAAGACT ACGCCAAGGT GTTGCTGTTG 
ATAGATGATG AATACAAGGT CACAGCTTTT CCAGCCACTC GGAATGTCTT GCGACAGCTA 
CATGAGCTTG CCCCTTCCAT CTTCTTCTAT TTGGTGGATG CAGAGCAGGG ACGGCTGTGT 
GGATATCGGC TTCGAAAGGA TCTCACCACT GAGCTGAGTT GGGAGCTGAC CATTCCCCCA 
GAAGTACAGC GGATCGTCAA GGTGAAGGGG AAACGCAGCA GTGAGCACGT TCATTCCCAG 
GGCCGTGTGA TGGGGGACCG CAGTGTGCTC TACAAGAGCC TGAACCCCAA CCTGCTGGCC 
GTGGTGACAG AGAGCACAGA CGCGCACCAT GAGCGCACCT TTATTGGCAT CTTCCTCATT 
GATGGCGTCA CTGGGCGTAT CATTCACTCC TCTGTGCAGA AGAAAGCCAA AGGCCCTGTC 
CATATCGTGC ATTCAGAGAA CTGGGTGGTG TACCAGTACT GGAACACCAA GGCTCGGCGC 
AACGAGTTTA CCGTACTGGA GCTCTATGAG GGCACTGAGC AATACAACGC CACCGCCTTC 
AGCTCCCTGG ACCGCCCCCA GCTGCCCCAG GTCCTCCAGC AGTCCTATAT CTTCCCGTCC 
TCCATCAGTG CCATGGAGGC CACCATCACC GAACGGGGCA TCACCAGCCG ACACCTGCTG 
ATTGGACTAC CTTCTGGAGC AATTCTTTCC CTTCCTAAGG CTTTGCTGGA TCCCCGCCGC 
CCCGAGATCC CAACAGAACA AAGCAGAGAG GAGAACTTAA TCCCGTATTC TCCAGATGTA 
CAGATACACG CAGAGCGATT CATCAACTAT AACCAGACAG TTTCTCGAAT GCGAGGTATC 
TACACAGCTC CCTCGGGTCT GGAGTCCACT TGTTTGGTTG TGGCCTATGG TTTGGACATT 
TACCAAACTC GAGTCTACCC ATCCAAGCAG TTTGACGTTC TGAAGGATGA CTATGACTAC 
GTGTTAATCA GCAGCGTCCT CTTTGGCCTG GTTTTTGCCA CCATGATCAC TAAGAGACTG 
GCACAGGTGA AGCTCCTGAA TCGGGCCTGG CGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258358.1
CDS	16..2931
Translation	MAAEWASRFWLWATLLIPAAAVYEDQVGKFDWRQQYVGKVKFASLEFSPGSKKLVVATEKNVIAALNSRTGEIYVITVSNGGRIMRSWETNIGGLNWEITLDSGSFQALGLVGLQESVRYIAVLKKTTLALHHLSSGHLKWVEHLPESDSIHYQMVYSYGSGVVWALGVVPFSHVNIVKFNVEDGEIVQQVRVSTPWLQHLSGACGVVDEAVLVCPDPSSRSLQTLALETEWELRQIPLQSLDLEFGSGFQPRVLPTQPNPVDASRAQFFLHLSPSHYALLQYHYGTLSLLKNFPQTALVSFATTGEKTVAAVMACRNEVQKSSSSEDGSMGSFSEKSSSKDSLACFNQTYTINLYLVETGRRLLDTTITFSLEQSGTRPERLYIQVFLKKDDSVGYRALVQTEDHLLLFLQQLAGKVVLWSREESLAEVVCLEMVDLPLTGAQAELEGEFGKKADGLLGMFLKRLSSQLILLQAWTSHLWKMFYDARKPRSQIKNEINIDTLARDEFNLQKMMVMVTASGKLFGIESSSGTILWKQYLPNVKPDSSFKLMVQRTTAHFPHPPQCTLLVKDKESGMSSLYVFNPIFGKWSQVAPPVLKRPILQSLLLPVMDQDYAKVLLLIDDEYKVTAFPATRNVLRQLHELAPSIFFYLVDAEQGRLCGYRLRKDLTTELSWELTIPPEVQRIVKVKGKRSSEHVHSQGRVMGDRSVLYKSLNPNLLAVVTESTDAHHERTFIGIFLIDGVTGRIIHSSVQKKAKGPVHIVHSENWVVYQYWNTKARRNEFTVLELYEGTEQYNATAFSSLDRPQLPQVLQQSYIFPSSISAMEATITERGITSRHLLIGLPSGAILSLPKALLDPRRPEIPTEQSREENLIPYSPDVQIHAERFINYNQTVSRMRGIYTAPSGLESTCLVVAYGLDIYQTRVYPSKQFDVLKDDYDYVLISSVLFGLVFATMITKRLAQVKLLNRAWR

Target ORF information:

RefSeq Version	NM_001271429.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271429.2

ORF Insert Sequence:

ATGGCGGCTG AGTGGGCTTC TCGTTTCTGG CTTTGGGCTA CGCTGCTGAT TCCTGCGGCC 
GCGGTCTACG AAGACCAAGT GGGCAAGTTT GATTGGAGAC AGCAATATGT TGGGAAGGTC 
AAGTTTGCCT CCTTGGAATT TTCCCCTGGA TCCAAGAAGT TGGTTGTAGC CACAGAGAAG 
AATGTGATTG CAGCATTAAA TTCCCGAACT GGGGAGATCT ATGTGATCAC TGTGTCCAAT 
GGAGGCCGAA TCATGCGTTC CTGGGAGACT AACATCGGGG GCCTGAACTG GGAGATAACC 
CTGGACAGTG GCAGTTTCCA GGCACTTGGG CTGGTTGGCC TGCAGGAGTC TGTAAGGTAC 
ATCGCAGTCC TGAAGAAGAC TACACTTGCC CTCCATCACC TCTCCAGTGG GCACCTCAAG 
TGGGTGGAAC ATCTCCCAGA AAGTGACAGC ATCCACTACC AGATGGTGTA TTCTTACGGC 
TCTGGGGTGG TGTGGGCCCT CGGAGTTGTT CCCTTCAGCC ATGTGAACAT TGTCAAGTTT 
AATGTGGAAG ATGGAGAGAT TGTTCAGCAG GTTAGGGTTT CAACTCCGTG GCTGCAGCAC 
CTGTCTGGAG CCTGTGGTGT GGTGGATGAG GCTGTCCTGG TGTGTCCTGA CCCGAGCTCA 
CGTTCCCTCC AAACTTTGGC TCTGGAGACG GAATGGGAGT TGAGACAGAT CCCACTGCAG 
TCTCTCGACT TAGAATTTGG AAGTGGATTC CAACCCCGGG TCCTGCCTAC CCAGCCCAAC 
CCAGTGGACG CTTCCCGGGC CCAGTTCTTC CTGCACTTGT CCCCAAGCCA CTATGCTCTG 
CTGCAGTACC ATTATGGAAC GCTGAGTTTG CTTAAAAACT TCCCACAGAC TGCCCTAGTG 
AGCTTTGCCA CCACTGGGGA GAAGACGGTG GCTGCAGTCA TGGCCTGTCG GAATGAAGTG 
CAGAAAAGTA GCAGTTCTGA AGATGGGTCA ATGGGGAGCT TTTCGGAGAA GTCTAGTTCA 
AAGGACTCTC TGGCTTGCTT CAATCAGACC TACACCATTA ACCTATACCT CGTGGAGACA 
GGTCGGCGGC TGCTGGACAC CACGATAACA TTTAGCCTGG AACAGAGCGG CACTCGGCCT 
GAGCGGCTGT ATATCCAGGT GTTCTTGAAG AAGGATGACT CAGTGGGCTA CCGGGCTTTG 
GTGCAGACAG AGGATCATCT GCTACTTTTC CTGCAGCAGT TGGCAGGGAA GGTGGTGCTG 
TGGAGCCGTG AGGAGTCCCT GGCAGAAGTG GTGTGCCTAG AGATGGTGGA CCTCCCCCTG 
ACTGGGGCAC AGGCCGAGCT GGAAGGAGAA TTTGGCAAAA AGGCAGATGG CTTGCTGGGG 
ATGTTCCTGA AACGCCTCTC GTCTCAGCTT ATCCTGCTGC AAGCATGGAC TTCCCACCTC 
TGGAAAATGT TTTATGATGC TCGGAAGCCC CGGAGTCAGA TTAAGAATGA GATCAACATT 
GACACCCTGG CCAGAGATGA ATTCAACCTC CAGAAGATGA TGGTGATGGT AACAGCCTCA 
GGCAAGCTTT TTGGCATTGA GAGCAGCTCT GGCACCATCC TGTGGAAACA GTATCTACCC 
AATGTCAAGC CAGACTCCTC CTTTAAACTG ATGGTCCAGA GAACTACTGC TCATTTCCCC 
CATCCCCCAC AGTGCACCCT GCTGGTGAAG GACAAGGAGT CGGGAATGAG TTCTCTGTAT 
GTCTTCAATC CCATTTTTGG GAAGTGGAGT CAGGTAGCTC CCCCAGTGCT GAAGCGCCCC 
ATCTTGCAGT CCTTGCTTCT CCCAGTCATG GATCAAGACT ACGCCAAGGT GTTGCTGTTG 
ATAGATGATG AATACAAGGT CACAGCTTTT CCAGCCACTC GGAATGTCTT GCGACAGCTA 
CATGAGCTTG CCCCTTCCAT CTTCTTCTAT TTGGTGGATG CAGAGCAGGG ACGGCTGTGT 
GGATATCGGC TTCGAAAGGA TCTCACCACT GAGCTGAGTT GGGAGCTGAC CATTCCCCCA 
GAAGTACAGC GGATCGTCAA GGTGAAGGGG AAACGCAGCA GTGAGCACGT TCATTCCCAG 
GGCCGTGTGA TGGGGGACCG CAGTGTGCTC TACAAGAGCC TGAACCCCAA CCTGCTGGCC 
GTGGTGACAG AGAGCACAGA CGCGCACCAT GAGCGCACCT TTATTGGCAT CTTCCTCATT 
GATGGCGTCA CTGGGCGTAT CATTCACTCC TCTGTGCAGA AGAAAGCCAA AGGCCCTGTC 
CATATCGTGC ATTCAGAGAA CTGGGTGGTG TACCAGTACT GGAACACCAA GGCTCGGCGC 
AACGAGTTTA CCGTACTGGA GCTCTATGAG GGCACTGAGC AATACAACGC CACCGCCTTC 
AGCTCCCTGG ACCGCCCCCA GCTGCCCCAG GTCCTCCAGC AGTCCTATAT CTTCCCGTCC 
TCCATCAGTG CCATGGAGGC CACCATCACC GAACGGGGCA TCACCAGCCG ACACCTGCTG 
ATTGGACTAC CTTCTGGAGC AATTCTTTCC CTTCCTAAGG CTTTGCTGGA TCCCCGCCGC 
CCCGAGATCC CAACAGAACA AAGCAGAGAG GAGAACTTAA TCCCGTATTC TCCAGATGTA 
CAGATACACG CAGAGCGATT CATCAACTAT AACCAGACAG TTTCTCGAAT GCGAGGTATC 
TACACAGCTC CCTCGGGTCT GGAGTCCACT TGTTTGGTTG TGGCCTATGG TTTGGACATT 
TACCAAACTC GAGTCTACCC ATCCAAGCAG TTTGACGTTC TGAAGGATGA CTATGACTAC 
GTGTTAATCA GCAGCGTCCT CTTTGGCCTG GTTTTTGCCA CCATGATCAC TAAGAGACTG 
GCACAGGTGA AGCTCCTGAA TCGGGCCTGG CGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMC1 ( XM_005245787.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005245844.1 Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant X1, mRNA.

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CloneID	OHu33200
Clone ID Related Accession (Same CDS sequence)	XM_005245787.1 , NM_001375820.1 , XM_005245787.2
Accession Version	XM_005245787.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2991bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ER membrane protein complex subunit 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG AGTGGGCTTC TCGTTTCTGG CTTTGGGCTA CGCTGCTGAT TCCTGCGGCC 
GCGGTCTACG AAGACCAAGT GGGCAAGTTT GATTGGAGAC AGCAATATGT TGGGAAGGTC 
AAGTTTGCCT CCTTGGAATT TTCCCCTGGA TCCAAGAAGT TGGTTGTAGC CACAGAGAAG 
AATGTGATTG CAGCATTAAA TTCCCGAACT GGGGAGATCT TGTGGCGCCA TGTTGACAAG 
GGCACGGCAG AAGGGGCTGT GGATGCCATG CTGCTGCACG GACAGGATGT GATCACTGTG 
TCCAATGGAG GCCGAATCAT GCGTTCCTGG GAGACTAACA TCGGGGGCCT GAACTGGGAG 
ATAACCCTGG ACAGTGGCAG TTTCCAGGCA CTTGGGCTGG TTGGCCTGCA GGAGTCTGTA 
AGGTACATCG CAGTCCTGAA GAAGACTACA CTTGCCCTCC ATCACCTCTC CAGTGGGCAC 
CTCAAGTGGG TGGAACATCT CCCAGAAAGT GACAGCATCC ACTACCAGAT GGTGTATTCT 
TACGGCTCTG GGGTGGTGTG GGCCCTCGGA GTTGTTCCCT TCAGCCATGT GAACATTGTC 
AAGTTTAATG TGGAAGATGG AGAGATTGTT CAGCAGGTTA GGGTTTCAAC TCCGTGGCTG 
CAGCACCTGT CTGGAGCCTG TGGTGTGGTG GATGAGGCTG TCCTGGTGTG TCCTGACCCG 
AGCTCACGTT CCCTCCAAAC TTTGGCTCTG GAGACGGAAT GGGAGTTGAG ACAGATCCCA 
CTGCAGTCTC TCGACTTAGA ATTTGGAAGT GGATTCCAAC CCCGGGTCCT GCCTACCCAG 
CCCAACCCAG TGGACGCTTC CCGGGCCCAG TTCTTCCTGC ACTTGTCCCC AAGCCACTAT 
GCTCTGCTGC AGTACCATTA TGGAACGCTG AGTTTGCTTA AAAACTTCCC ACAGACTGCC 
CTAGTGAGCT TTGCCACCAC TGGGGAGAAG ACGGTGGCTG CAGTCATGGC CTGTCGGAAT 
GAAGTGCAGA AAAGTAGCAG TTCTGAAGAT GGGTCAATGG GGAGCTTTTC GGAGAAGTCT 
AGTTCAAAGG ACTCTCTGGC TTGCTTCAAT CAGACCTACA CCATTAACCT ATACCTCGTG 
GAGACAGGTC GGCGGCTGCT GGACACCACG ATAACATTTA GCCTGGAACA GAGCGGCACT 
CGGCCTGAGC GGCTGTATAT CCAGGTGTTC TTGAAGAAGG ATGACTCAGT GGGCTACCGG 
GCTTTGGTGC AGACAGAGGA TCATCTGCTA CTTTTCCTGC AGCAGTTGGC AGGGAAGGTG 
GTGCTGTGGA GCCGTGAGGA GTCCCTGGCA GAAGTGGTGT GCCTAGAGAT GGTGGACCTC 
CCCCTGACTG GGGCACAGGC CGAGCTGGAA GGAGAATTTG GCAAAAAGGC AGCAATTCAA 
GATGGCTTGC TGGGGATGTT CCTGAAACGC CTCTCGTCTC AGCTTATCCT GCTGCAAGCA 
TGGACTTCCC ACCTCTGGAA AATGTTTTAT GATGCTCGGA AGCCCCGGAG TCAGATTAAG 
AATGAGATCA ACATTGACAC CCTGGCCAGA GATGAATTCA ACCTCCAGAA GATGATGGTG 
ATGGTAACAG CCTCAGGCAA GCTTTTTGGC ATTGAGAGCA GCTCTGGCAC CATCCTGTGG 
AAACAGTATC TACCCAATGT CAAGCCAGAC TCCTCCTTTA AACTGATGGT CCAGAGAACT 
ACTGCTCATT TCCCCCATCC CCCACAGTGC ACCCTGCTGG TGAAGGACAA GGAGTCGGGA 
ATGAGTTCTC TGTATGTCTT CAATCCCATT TTTGGGAAGT GGAGTCAGGT AGCTCCCCCA 
GTGCTGAAGC GCCCCATCTT GCAGTCCTTG CTTCTCCCAG TCATGGATCA AGACTACGCC 
AAGGTGTTGC TGTTGATAGA TGATGAATAC AAGGTCACAG CTTTTCCAGC CACTCGGAAT 
GTCTTGCGAC AGCTACATGA GCTTGCCCCT TCCATCTTCT TCTATTTGGT GGATGCAGAG 
CAGGGACGGC TGTGTGGATA TCGGCTTCGA AAGGATCTCA CCACTGAGCT GAGTTGGGAG 
CTGACCATTC CCCCAGAAGT ACAGCGGATC GTCAAGGTGA AGGGGAAACG CAGCAGTGAG 
CACGTTCATT CCCAGGGCCG TGTGATGGGG GACCGCAGTG TGCTCTACAA GAGCCTGAAC 
CCCAACCTGC TGGCCGTGGT GACAGAGAGC ACAGACGCGC ACCATGAGCG CACCTTTATT 
GGCATCTTCC TCATTGATGG CGTCACTGGG CGTATCATTC ACTCCTCTGT GCAGAAGAAA 
GCCAAAGGCC CTGTCCATAT CGTGCATTCA GAGAACTGGG TGGTGTACCA GTACTGGAAC 
ACCAAGGCTC GGCGCAACGA GTTTACCGTA CTGGAGCTCT ATGAGGGCAC TGAGCAATAC 
AACGCCACCG CCTTCAGCTC CCTGGACCGC CCCCAGCTGC CCCAGGTCCT CCAGCAGTCC 
TATATCTTCC CGTCCTCCAT CAGTGCCATG GAGGCCACCA TCACCGAACG GGGCATCACC 
AGCCGACACC TGCTGATTGG ACTACCTTCT GGAGCAATTC TTTCCCTTCC TAAGGCTTTG 
CTGGATCCCC GCCGCCCCGA GATCCCAACA GAACAAAGCA GAGAGGAGAA CTTAATCCCG 
TATTCTCCAG ATGTACAGAT ACACGCAGAG CGATTCATCA ACTATAACCA GACAGTTTCT 
CGAATGCGAG GTATCTACAC AGCTCCCTCG GGTCTGGAGT CCACTTGTTT GGTTGTGGCC 
TATGGTTTGG ACATTTACCA AACTCGAGTC TACCCATCCA AGCAGTTTGA CGTTCTGAAG 
GATGACTATG ACTACGTGTT AATCAGCAGC GTCCTCTTTG GCCTGGTTTT TGCCACCATG 
ATCACTAAGA GACTGGCACA GGTGAAGCTC CTGAATCGGG CCTGGCGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005245844.1
CDS	46..3036
Translation	MAAEWASRFWLWATLLIPAAAVYEDQVGKFDWRQQYVGKVKFASLEFSPGSKKLVVATEKNVIAALNSRTGEILWRHVDKGTAEGAVDAMLLHGQDVITVSNGGRIMRSWETNIGGLNWEITLDSGSFQALGLVGLQESVRYIAVLKKTTLALHHLSSGHLKWVEHLPESDSIHYQMVYSYGSGVVWALGVVPFSHVNIVKFNVEDGEIVQQVRVSTPWLQHLSGACGVVDEAVLVCPDPSSRSLQTLALETEWELRQIPLQSLDLEFGSGFQPRVLPTQPNPVDASRAQFFLHLSPSHYALLQYHYGTLSLLKNFPQTALVSFATTGEKTVAAVMACRNEVQKSSSSEDGSMGSFSEKSSSKDSLACFNQTYTINLYLVETGRRLLDTTITFSLEQSGTRPERLYIQVFLKKDDSVGYRALVQTEDHLLLFLQQLAGKVVLWSREESLAEVVCLEMVDLPLTGAQAELEGEFGKKAAIQDGLLGMFLKRLSSQLILLQAWTSHLWKMFYDARKPRSQIKNEINIDTLARDEFNLQKMMVMVTASGKLFGIESSSGTILWKQYLPNVKPDSSFKLMVQRTTAHFPHPPQCTLLVKDKESGMSSLYVFNPIFGKWSQVAPPVLKRPILQSLLLPVMDQDYAKVLLLIDDEYKVTAFPATRNVLRQLHELAPSIFFYLVDAEQGRLCGYRLRKDLTTELSWELTIPPEVQRIVKVKGKRSSEHVHSQGRVMGDRSVLYKSLNPNLLAVVTESTDAHHERTFIGIFLIDGVTGRIIHSSVQKKAKGPVHIVHSENWVVYQYWNTKARRNEFTVLELYEGTEQYNATAFSSLDRPQLPQVLQQSYIFPSSISAMEATITERGITSRHLLIGLPSGAILSLPKALLDPRRPEIPTEQSREENLIPYSPDVQIHAERFINYNQTVSRMRGIYTAPSGLESTCLVVAYGLDIYQTRVYPSKQFDVLKDDYDYVLISSVLFGLVFATMITKRLAQVKLLNRAWR

Target ORF information:

RefSeq Version	XM_005245787.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005245787.1

ORF Insert Sequence:

ATGGCGGCTG AGTGGGCTTC TCGTTTCTGG CTTTGGGCTA CGCTGCTGAT TCCTGCGGCC 
GCGGTCTACG AAGACCAAGT GGGCAAGTTT GATTGGAGAC AGCAATATGT TGGGAAGGTC 
AAGTTTGCCT CCTTGGAATT TTCCCCTGGA TCCAAGAAGT TGGTTGTAGC CACAGAGAAG 
AATGTGATTG CAGCATTAAA TTCCCGAACT GGGGAGATCT TGTGGCGCCA TGTTGACAAG 
GGCACGGCAG AAGGGGCTGT GGATGCCATG CTGCTGCACG GACAGGATGT GATCACTGTG 
TCCAATGGAG GCCGAATCAT GCGTTCCTGG GAGACTAACA TCGGGGGCCT GAACTGGGAG 
ATAACCCTGG ACAGTGGCAG TTTCCAGGCA CTTGGGCTGG TTGGCCTGCA GGAGTCTGTA 
AGGTACATCG CAGTCCTGAA GAAGACTACA CTTGCCCTCC ATCACCTCTC CAGTGGGCAC 
CTCAAGTGGG TGGAACATCT CCCAGAAAGT GACAGCATCC ACTACCAGAT GGTGTATTCT 
TACGGCTCTG GGGTGGTGTG GGCCCTCGGA GTTGTTCCCT TCAGCCATGT GAACATTGTC 
AAGTTTAATG TGGAAGATGG AGAGATTGTT CAGCAGGTTA GGGTTTCAAC TCCGTGGCTG 
CAGCACCTGT CTGGAGCCTG TGGTGTGGTG GATGAGGCTG TCCTGGTGTG TCCTGACCCG 
AGCTCACGTT CCCTCCAAAC TTTGGCTCTG GAGACGGAAT GGGAGTTGAG ACAGATCCCA 
CTGCAGTCTC TCGACTTAGA ATTTGGAAGT GGATTCCAAC CCCGGGTCCT GCCTACCCAG 
CCCAACCCAG TGGACGCTTC CCGGGCCCAG TTCTTCCTGC ACTTGTCCCC AAGCCACTAT 
GCTCTGCTGC AGTACCATTA TGGAACGCTG AGTTTGCTTA AAAACTTCCC ACAGACTGCC 
CTAGTGAGCT TTGCCACCAC TGGGGAGAAG ACGGTGGCTG CAGTCATGGC CTGTCGGAAT 
GAAGTGCAGA AAAGTAGCAG TTCTGAAGAT GGGTCAATGG GGAGCTTTTC GGAGAAGTCT 
AGTTCAAAGG ACTCTCTGGC TTGCTTCAAT CAGACCTACA CCATTAACCT ATACCTCGTG 
GAGACAGGTC GGCGGCTGCT GGACACCACG ATAACATTTA GCCTGGAACA GAGCGGCACT 
CGGCCTGAGC GGCTGTATAT CCAGGTGTTC TTGAAGAAGG ATGACTCAGT GGGCTACCGG 
GCTTTGGTGC AGACAGAGGA TCATCTGCTA CTTTTCCTGC AGCAGTTGGC AGGGAAGGTG 
GTGCTGTGGA GCCGTGAGGA GTCCCTGGCA GAAGTGGTGT GCCTAGAGAT GGTGGACCTC 
CCCCTGACTG GGGCACAGGC CGAGCTGGAA GGAGAATTTG GCAAAAAGGC AGCAATTCAA 
GATGGCTTGC TGGGGATGTT CCTGAAACGC CTCTCGTCTC AGCTTATCCT GCTGCAAGCA 
TGGACTTCCC ACCTCTGGAA AATGTTTTAT GATGCTCGGA AGCCCCGGAG TCAGATTAAG 
AATGAGATCA ACATTGACAC CCTGGCCAGA GATGAATTCA ACCTCCAGAA GATGATGGTG 
ATGGTAACAG CCTCAGGCAA GCTTTTTGGC ATTGAGAGCA GCTCTGGCAC CATCCTGTGG 
AAACAGTATC TACCCAATGT CAAGCCAGAC TCCTCCTTTA AACTGATGGT CCAGAGAACT 
ACTGCTCATT TCCCCCATCC CCCACAGTGC ACCCTGCTGG TGAAGGACAA GGAGTCGGGA 
ATGAGTTCTC TGTATGTCTT CAATCCCATT TTTGGGAAGT GGAGTCAGGT AGCTCCCCCA 
GTGCTGAAGC GCCCCATCTT GCAGTCCTTG CTTCTCCCAG TCATGGATCA AGACTACGCC 
AAGGTGTTGC TGTTGATAGA TGATGAATAC AAGGTCACAG CTTTTCCAGC CACTCGGAAT 
GTCTTGCGAC AGCTACATGA GCTTGCCCCT TCCATCTTCT TCTATTTGGT GGATGCAGAG 
CAGGGACGGC TGTGTGGATA TCGGCTTCGA AAGGATCTCA CCACTGAGCT GAGTTGGGAG 
CTGACCATTC CCCCAGAAGT ACAGCGGATC GTCAAGGTGA AGGGGAAACG CAGCAGTGAG 
CACGTTCATT CCCAGGGCCG TGTGATGGGG GACCGCAGTG TGCTCTACAA GAGCCTGAAC 
CCCAACCTGC TGGCCGTGGT GACAGAGAGC ACAGACGCGC ACCATGAGCG CACCTTTATT 
GGCATCTTCC TCATTGATGG CGTCACTGGG CGTATCATTC ACTCCTCTGT GCAGAAGAAA 
GCCAAAGGCC CTGTCCATAT CGTGCATTCA GAGAACTGGG TGGTGTACCA GTACTGGAAC 
ACCAAGGCTC GGCGCAACGA GTTTACCGTA CTGGAGCTCT ATGAGGGCAC TGAGCAATAC 
AACGCCACCG CCTTCAGCTC CCTGGACCGC CCCCAGCTGC CCCAGGTCCT CCAGCAGTCC 
TATATCTTCC CGTCCTCCAT CAGTGCCATG GAGGCCACCA TCACCGAACG GGGCATCACC 
AGCCGACACC TGCTGATTGG ACTACCTTCT GGAGCAATTC TTTCCCTTCC TAAGGCTTTG 
CTGGATCCCC GCCGCCCCGA GATCCCAACA GAACAAAGCA GAGAGGAGAA CTTAATCCCG 
TATTCTCCAG ATGTACAGAT ACACGCAGAG CGATTCATCA ACTATAACCA GACAGTTTCT 
CGAATGCGAG GTATCTACAC AGCTCCCTCG GGTCTGGAGT CCACTTGTTT GGTTGTGGCC 
TATGGTTTGG ACATTTACCA AACTCGAGTC TACCCATCCA AGCAGTTTGA CGTTCTGAAG 
GATGACTATG ACTACGTGTT AATCAGCAGC GTCCTCTTTG GCCTGGTTTT TGCCACCATG 
ATCACTAAGA GACTGGCACA GGTGAAGCTC CTGAATCGGG CCTGGCGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMC1 ( XM_005245787.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005245844.1 Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu33200
Clone ID Related Accession (Same CDS sequence)	XM_005245787.1 , NM_001375820.1 , XM_005245787.2
Accession Version	XM_005245787.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2991bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	ER membrane protein complex subunit 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005245787.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGGCGGCTG AGTGGGCTTC TCGTTTCTGG CTTTGGGCTA CGCTGCTGAT TCCTGCGGCC 
GCGGTCTACG AAGACCAAGT GGGCAAGTTT GATTGGAGAC AGCAATATGT TGGGAAGGTC 
AAGTTTGCCT CCTTGGAATT TTCCCCTGGA TCCAAGAAGT TGGTTGTAGC CACAGAGAAG 
AATGTGATTG CAGCATTAAA TTCCCGAACT GGGGAGATCT TGTGGCGCCA TGTTGACAAG 
GGCACGGCAG AAGGGGCTGT GGATGCCATG CTGCTGCACG GACAGGATGT GATCACTGTG 
TCCAATGGAG GCCGAATCAT GCGTTCCTGG GAGACTAACA TCGGGGGCCT GAACTGGGAG 
ATAACCCTGG ACAGTGGCAG TTTCCAGGCA CTTGGGCTGG TTGGCCTGCA GGAGTCTGTA 
AGGTACATCG CAGTCCTGAA GAAGACTACA CTTGCCCTCC ATCACCTCTC CAGTGGGCAC 
CTCAAGTGGG TGGAACATCT CCCAGAAAGT GACAGCATCC ACTACCAGAT GGTGTATTCT 
TACGGCTCTG GGGTGGTGTG GGCCCTCGGA GTTGTTCCCT TCAGCCATGT GAACATTGTC 
AAGTTTAATG TGGAAGATGG AGAGATTGTT CAGCAGGTTA GGGTTTCAAC TCCGTGGCTG 
CAGCACCTGT CTGGAGCCTG TGGTGTGGTG GATGAGGCTG TCCTGGTGTG TCCTGACCCG 
AGCTCACGTT CCCTCCAAAC TTTGGCTCTG GAGACGGAAT GGGAGTTGAG ACAGATCCCA 
CTGCAGTCTC TCGACTTAGA ATTTGGAAGT GGATTCCAAC CCCGGGTCCT GCCTACCCAG 
CCCAACCCAG TGGACGCTTC CCGGGCCCAG TTCTTCCTGC ACTTGTCCCC AAGCCACTAT 
GCTCTGCTGC AGTACCATTA TGGAACGCTG AGTTTGCTTA AAAACTTCCC ACAGACTGCC 
CTAGTGAGCT TTGCCACCAC TGGGGAGAAG ACGGTGGCTG CAGTCATGGC CTGTCGGAAT 
GAAGTGCAGA AAAGTAGCAG TTCTGAAGAT GGGTCAATGG GGAGCTTTTC GGAGAAGTCT 
AGTTCAAAGG ACTCTCTGGC TTGCTTCAAT CAGACCTACA CCATTAACCT ATACCTCGTG 
GAGACAGGTC GGCGGCTGCT GGACACCACG ATAACATTTA GCCTGGAACA GAGCGGCACT 
CGGCCTGAGC GGCTGTATAT CCAGGTGTTC TTGAAGAAGG ATGACTCAGT GGGCTACCGG 
GCTTTGGTGC AGACAGAGGA TCATCTGCTA CTTTTCCTGC AGCAGTTGGC AGGGAAGGTG 
GTGCTGTGGA GCCGTGAGGA GTCCCTGGCA GAAGTGGTGT GCCTAGAGAT GGTGGACCTC 
CCCCTGACTG GGGCACAGGC CGAGCTGGAA GGAGAATTTG GCAAAAAGGC AGCAATTCAA 
GATGGCTTGC TGGGGATGTT CCTGAAACGC CTCTCGTCTC AGCTTATCCT GCTGCAAGCA 
TGGACTTCCC ACCTCTGGAA AATGTTTTAT GATGCTCGGA AGCCCCGGAG TCAGATTAAG 
AATGAGATCA ACATTGACAC CCTGGCCAGA GATGAATTCA ACCTCCAGAA GATGATGGTG 
ATGGTAACAG CCTCAGGCAA GCTTTTTGGC ATTGAGAGCA GCTCTGGCAC CATCCTGTGG 
AAACAGTATC TACCCAATGT CAAGCCAGAC TCCTCCTTTA AACTGATGGT CCAGAGAACT 
ACTGCTCATT TCCCCCATCC CCCACAGTGC ACCCTGCTGG TGAAGGACAA GGAGTCGGGA 
ATGAGTTCTC TGTATGTCTT CAATCCCATT TTTGGGAAGT GGAGTCAGGT AGCTCCCCCA 
GTGCTGAAGC GCCCCATCTT GCAGTCCTTG CTTCTCCCAG TCATGGATCA AGACTACGCC 
AAGGTGTTGC TGTTGATAGA TGATGAATAC AAGGTCACAG CTTTTCCAGC CACTCGGAAT 
GTCTTGCGAC AGCTACATGA GCTTGCCCCT TCCATCTTCT TCTATTTGGT GGATGCAGAG 
CAGGGACGGC TGTGTGGATA TCGGCTTCGA AAGGATCTCA CCACTGAGCT GAGTTGGGAG 
CTGACCATTC CCCCAGAAGT ACAGCGGATC GTCAAGGTGA AGGGGAAACG CAGCAGTGAG 
CACGTTCATT CCCAGGGCCG TGTGATGGGG GACCGCAGTG TGCTCTACAA GAGCCTGAAC 
CCCAACCTGC TGGCCGTGGT GACAGAGAGC ACAGACGCGC ACCATGAGCG CACCTTTATT 
GGCATCTTCC TCATTGATGG CGTCACTGGG CGTATCATTC ACTCCTCTGT GCAGAAGAAA 
GCCAAAGGCC CTGTCCATAT CGTGCATTCA GAGAACTGGG TGGTGTACCA GTACTGGAAC 
ACCAAGGCTC GGCGCAACGA GTTTACCGTA CTGGAGCTCT ATGAGGGCAC TGAGCAATAC 
AACGCCACCG CCTTCAGCTC CCTGGACCGC CCCCAGCTGC CCCAGGTCCT CCAGCAGTCC 
TATATCTTCC CGTCCTCCAT CAGTGCCATG GAGGCCACCA TCACCGAACG GGGCATCACC 
AGCCGACACC TGCTGATTGG ACTACCTTCT GGAGCAATTC TTTCCCTTCC TAAGGCTTTG 
CTGGATCCCC GCCGCCCCGA GATCCCAACA GAACAAAGCA GAGAGGAGAA CTTAATCCCG 
TATTCTCCAG ATGTACAGAT ACACGCAGAG CGATTCATCA ACTATAACCA GACAGTTTCT 
CGAATGCGAG GTATCTACAC AGCTCCCTCG GGTCTGGAGT CCACTTGTTT GGTTGTGGCC 
TATGGTTTGG ACATTTACCA AACTCGAGTC TACCCATCCA AGCAGTTTGA CGTTCTGAAG 
GATGACTATG ACTACGTGTT AATCAGCAGC GTCCTCTTTG GCCTGGTTTT TGCCACCATG 
ATCACTAAGA GACTGGCACA GGTGAAGCTC CTGAATCGGG CCTGGCGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005245844.1
CDS	39..3029
Translation	MAAEWASRFWLWATLLIPAAAVYEDQVGKFDWRQQYVGKVKFASLEFSPGSKKLVVATEKNVIAALNSRTGEILWRHVDKGTAEGAVDAMLLHGQDVITVSNGGRIMRSWETNIGGLNWEITLDSGSFQALGLVGLQESVRYIAVLKKTTLALHHLSSGHLKWVEHLPESDSIHYQMVYSYGSGVVWALGVVPFSHVNIVKFNVEDGEIVQQVRVSTPWLQHLSGACGVVDEAVLVCPDPSSRSLQTLALETEWELRQIPLQSLDLEFGSGFQPRVLPTQPNPVDASRAQFFLHLSPSHYALLQYHYGTLSLLKNFPQTALVSFATTGEKTVAAVMACRNEVQKSSSSEDGSMGSFSEKSSSKDSLACFNQTYTINLYLVETGRRLLDTTITFSLEQSGTRPERLYIQVFLKKDDSVGYRALVQTEDHLLLFLQQLAGKVVLWSREESLAEVVCLEMVDLPLTGAQAELEGEFGKKAAIQDGLLGMFLKRLSSQLILLQAWTSHLWKMFYDARKPRSQIKNEINIDTLARDEFNLQKMMVMVTASGKLFGIESSSGTILWKQYLPNVKPDSSFKLMVQRTTAHFPHPPQCTLLVKDKESGMSSLYVFNPIFGKWSQVAPPVLKRPILQSLLLPVMDQDYAKVLLLIDDEYKVTAFPATRNVLRQLHELAPSIFFYLVDAEQGRLCGYRLRKDLTTELSWELTIPPEVQRIVKVKGKRSSEHVHSQGRVMGDRSVLYKSLNPNLLAVVTESTDAHHERTFIGIFLIDGVTGRIIHSSVQKKAKGPVHIVHSENWVVYQYWNTKARRNEFTVLELYEGTEQYNATAFSSLDRPQLPQVLQQSYIFPSSISAMEATITERGITSRHLLIGLPSGAILSLPKALLDPRRPEIPTEQSREENLIPYSPDVQIHAERFINYNQTVSRMRGIYTAPSGLESTCLVVAYGLDIYQTRVYPSKQFDVLKDDYDYVLISSVLFGLVFATMITKRLAQVKLLNRAWR

Target ORF information:

RefSeq Version	XM_005245787.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005245787.2

ORF Insert Sequence:

ATGGCGGCTG AGTGGGCTTC TCGTTTCTGG CTTTGGGCTA CGCTGCTGAT TCCTGCGGCC 
GCGGTCTACG AAGACCAAGT GGGCAAGTTT GATTGGAGAC AGCAATATGT TGGGAAGGTC 
AAGTTTGCCT CCTTGGAATT TTCCCCTGGA TCCAAGAAGT TGGTTGTAGC CACAGAGAAG 
AATGTGATTG CAGCATTAAA TTCCCGAACT GGGGAGATCT TGTGGCGCCA TGTTGACAAG 
GGCACGGCAG AAGGGGCTGT GGATGCCATG CTGCTGCACG GACAGGATGT GATCACTGTG 
TCCAATGGAG GCCGAATCAT GCGTTCCTGG GAGACTAACA TCGGGGGCCT GAACTGGGAG 
ATAACCCTGG ACAGTGGCAG TTTCCAGGCA CTTGGGCTGG TTGGCCTGCA GGAGTCTGTA 
AGGTACATCG CAGTCCTGAA GAAGACTACA CTTGCCCTCC ATCACCTCTC CAGTGGGCAC 
CTCAAGTGGG TGGAACATCT CCCAGAAAGT GACAGCATCC ACTACCAGAT GGTGTATTCT 
TACGGCTCTG GGGTGGTGTG GGCCCTCGGA GTTGTTCCCT TCAGCCATGT GAACATTGTC 
AAGTTTAATG TGGAAGATGG AGAGATTGTT CAGCAGGTTA GGGTTTCAAC TCCGTGGCTG 
CAGCACCTGT CTGGAGCCTG TGGTGTGGTG GATGAGGCTG TCCTGGTGTG TCCTGACCCG 
AGCTCACGTT CCCTCCAAAC TTTGGCTCTG GAGACGGAAT GGGAGTTGAG ACAGATCCCA 
CTGCAGTCTC TCGACTTAGA ATTTGGAAGT GGATTCCAAC CCCGGGTCCT GCCTACCCAG 
CCCAACCCAG TGGACGCTTC CCGGGCCCAG TTCTTCCTGC ACTTGTCCCC AAGCCACTAT 
GCTCTGCTGC AGTACCATTA TGGAACGCTG AGTTTGCTTA AAAACTTCCC ACAGACTGCC 
CTAGTGAGCT TTGCCACCAC TGGGGAGAAG ACGGTGGCTG CAGTCATGGC CTGTCGGAAT 
GAAGTGCAGA AAAGTAGCAG TTCTGAAGAT GGGTCAATGG GGAGCTTTTC GGAGAAGTCT 
AGTTCAAAGG ACTCTCTGGC TTGCTTCAAT CAGACCTACA CCATTAACCT ATACCTCGTG 
GAGACAGGTC GGCGGCTGCT GGACACCACG ATAACATTTA GCCTGGAACA GAGCGGCACT 
CGGCCTGAGC GGCTGTATAT CCAGGTGTTC TTGAAGAAGG ATGACTCAGT GGGCTACCGG 
GCTTTGGTGC AGACAGAGGA TCATCTGCTA CTTTTCCTGC AGCAGTTGGC AGGGAAGGTG 
GTGCTGTGGA GCCGTGAGGA GTCCCTGGCA GAAGTGGTGT GCCTAGAGAT GGTGGACCTC 
CCCCTGACTG GGGCACAGGC CGAGCTGGAA GGAGAATTTG GCAAAAAGGC AGCAATTCAA 
GATGGCTTGC TGGGGATGTT CCTGAAACGC CTCTCGTCTC AGCTTATCCT GCTGCAAGCA 
TGGACTTCCC ACCTCTGGAA AATGTTTTAT GATGCTCGGA AGCCCCGGAG TCAGATTAAG 
AATGAGATCA ACATTGACAC CCTGGCCAGA GATGAATTCA ACCTCCAGAA GATGATGGTG 
ATGGTAACAG CCTCAGGCAA GCTTTTTGGC ATTGAGAGCA GCTCTGGCAC CATCCTGTGG 
AAACAGTATC TACCCAATGT CAAGCCAGAC TCCTCCTTTA AACTGATGGT CCAGAGAACT 
ACTGCTCATT TCCCCCATCC CCCACAGTGC ACCCTGCTGG TGAAGGACAA GGAGTCGGGA 
ATGAGTTCTC TGTATGTCTT CAATCCCATT TTTGGGAAGT GGAGTCAGGT AGCTCCCCCA 
GTGCTGAAGC GCCCCATCTT GCAGTCCTTG CTTCTCCCAG TCATGGATCA AGACTACGCC 
AAGGTGTTGC TGTTGATAGA TGATGAATAC AAGGTCACAG CTTTTCCAGC CACTCGGAAT 
GTCTTGCGAC AGCTACATGA GCTTGCCCCT TCCATCTTCT TCTATTTGGT GGATGCAGAG 
CAGGGACGGC TGTGTGGATA TCGGCTTCGA AAGGATCTCA CCACTGAGCT GAGTTGGGAG 
CTGACCATTC CCCCAGAAGT ACAGCGGATC GTCAAGGTGA AGGGGAAACG CAGCAGTGAG 
CACGTTCATT CCCAGGGCCG TGTGATGGGG GACCGCAGTG TGCTCTACAA GAGCCTGAAC 
CCCAACCTGC TGGCCGTGGT GACAGAGAGC ACAGACGCGC ACCATGAGCG CACCTTTATT 
GGCATCTTCC TCATTGATGG CGTCACTGGG CGTATCATTC ACTCCTCTGT GCAGAAGAAA 
GCCAAAGGCC CTGTCCATAT CGTGCATTCA GAGAACTGGG TGGTGTACCA GTACTGGAAC 
ACCAAGGCTC GGCGCAACGA GTTTACCGTA CTGGAGCTCT ATGAGGGCAC TGAGCAATAC 
AACGCCACCG CCTTCAGCTC CCTGGACCGC CCCCAGCTGC CCCAGGTCCT CCAGCAGTCC 
TATATCTTCC CGTCCTCCAT CAGTGCCATG GAGGCCACCA TCACCGAACG GGGCATCACC 
AGCCGACACC TGCTGATTGG ACTACCTTCT GGAGCAATTC TTTCCCTTCC TAAGGCTTTG 
CTGGATCCCC GCCGCCCCGA GATCCCAACA GAACAAAGCA GAGAGGAGAA CTTAATCCCG 
TATTCTCCAG ATGTACAGAT ACACGCAGAG CGATTCATCA ACTATAACCA GACAGTTTCT 
CGAATGCGAG GTATCTACAC AGCTCCCTCG GGTCTGGAGT CCACTTGTTT GGTTGTGGCC 
TATGGTTTGG ACATTTACCA AACTCGAGTC TACCCATCCA AGCAGTTTGA CGTTCTGAAG 
GATGACTATG ACTACGTGTT AATCAGCAGC GTCCTCTTTG GCCTGGTTTT TGCCACCATG 
ATCACTAAGA GACTGGCACA GGTGAAGCTC CTGAATCGGG CCTGGCGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMC1 ( XM_005245788.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005245845.1 Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu33201
Clone ID Related Accession (Same CDS sequence)	NM_001375821.1 , XM_005245788.1 , XM_005245788.2
Accession Version	XM_005245788.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2988bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ER membrane protein complex subunit 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCTG AGTGGGCTTC TCGTTTCTGG CTTTGGGCTA CGCTGCTGAT TCCTGCGGCC 
GCGGTCTACG AAGACCAAGT GGGCAAGTTT GATTGGAGAC AGCAATATGT TGGGAAGGTC 
AAGTTTGCCT CCTTGGAATT TTCCCCTGGA TCCAAGAAGT TGGTTGTAGC CACAGAGAAG 
AATGTGATTG CAGCATTAAA TTCCCGAACT GGGGAGATCT TGTGGCGCCA TGTTGACAAG 
GGCACGGCAG AAGGGGCTGT GGATGCCATG CTGCTGCACG GACAGGATGT GATCACTGTG 
TCCAATGGAG GCCGAATCAT GCGTTCCTGG GAGACTAACA TCGGGGGCCT GAACTGGGAG 
ATAACCCTGG ACAGTGGCAG TTTCCAGGCA CTTGGGCTGG TTGGCCTGCA GGAGTCTGTA 
AGGTACATCG CAGTCCTGAA GAAGACTACA CTTGCCCTCC ATCACCTCTC CAGTGGGCAC 
CTCAAGTGGG TGGAACATCT CCCAGAAAGT GACAGCATCC ACTACCAGAT GGTGTATTCT 
TACGGCTCTG GGGTGGTGTG GGCCCTCGGA GTTGTTCCCT TCAGCCATGT GAACATTGTC 
AAGTTTAATG TGGAAGATGG AGAGATTGTT CAGCAGGTTA GGGTTTCAAC TCCGTGGCTG 
CAGCACCTGT CTGGAGCCTG TGGTGTGGTG GATGAGGCTG TCCTGGTGTG TCCTGACCCG 
AGCTCACGTT CCCTCCAAAC TTTGGCTCTG GAGACGGAAT GGGAGTTGAG ACAGATCCCA 
CTGCAGTCTC TCGACTTAGA ATTTGGAAGT GGATTCCAAC CCCGGGTCCT GCCTACCCAG 
CCCAACCCAG TGGACGCTTC CCGGGCCCAG TTCTTCCTGC ACTTGTCCCC AAGCCACTAT 
GCTCTGCTGC AGTACCATTA TGGAACGCTG AGTTTGCTTA AAAACTTCCC ACAGACTGCC 
CTAGTGAGCT TTGCCACCAC TGGGGAGAAG ACGGTGGCTG CAGTCATGGC CTGTCGGAAT 
GAAGTGAAAA GTAGCAGTTC TGAAGATGGG TCAATGGGGA GCTTTTCGGA GAAGTCTAGT 
TCAAAGGACT CTCTGGCTTG CTTCAATCAG ACCTACACCA TTAACCTATA CCTCGTGGAG 
ACAGGTCGGC GGCTGCTGGA CACCACGATA ACATTTAGCC TGGAACAGAG CGGCACTCGG 
CCTGAGCGGC TGTATATCCA GGTGTTCTTG AAGAAGGATG ACTCAGTGGG CTACCGGGCT 
TTGGTGCAGA CAGAGGATCA TCTGCTACTT TTCCTGCAGC AGTTGGCAGG GAAGGTGGTG 
CTGTGGAGCC GTGAGGAGTC CCTGGCAGAA GTGGTGTGCC TAGAGATGGT GGACCTCCCC 
CTGACTGGGG CACAGGCCGA GCTGGAAGGA GAATTTGGCA AAAAGGCAGC AATTCAAGAT 
GGCTTGCTGG GGATGTTCCT GAAACGCCTC TCGTCTCAGC TTATCCTGCT GCAAGCATGG 
ACTTCCCACC TCTGGAAAAT GTTTTATGAT GCTCGGAAGC CCCGGAGTCA GATTAAGAAT 
GAGATCAACA TTGACACCCT GGCCAGAGAT GAATTCAACC TCCAGAAGAT GATGGTGATG 
GTAACAGCCT CAGGCAAGCT TTTTGGCATT GAGAGCAGCT CTGGCACCAT CCTGTGGAAA 
CAGTATCTAC CCAATGTCAA GCCAGACTCC TCCTTTAAAC TGATGGTCCA GAGAACTACT 
GCTCATTTCC CCCATCCCCC ACAGTGCACC CTGCTGGTGA AGGACAAGGA GTCGGGAATG 
AGTTCTCTGT ATGTCTTCAA TCCCATTTTT GGGAAGTGGA GTCAGGTAGC TCCCCCAGTG 
CTGAAGCGCC CCATCTTGCA GTCCTTGCTT CTCCCAGTCA TGGATCAAGA CTACGCCAAG 
GTGTTGCTGT TGATAGATGA TGAATACAAG GTCACAGCTT TTCCAGCCAC TCGGAATGTC 
TTGCGACAGC TACATGAGCT TGCCCCTTCC ATCTTCTTCT ATTTGGTGGA TGCAGAGCAG 
GGACGGCTGT GTGGATATCG GCTTCGAAAG GATCTCACCA CTGAGCTGAG TTGGGAGCTG 
ACCATTCCCC CAGAAGTACA GCGGATCGTC AAGGTGAAGG GGAAACGCAG CAGTGAGCAC 
GTTCATTCCC AGGGCCGTGT GATGGGGGAC CGCAGTGTGC TCTACAAGAG CCTGAACCCC 
AACCTGCTGG CCGTGGTGAC AGAGAGCACA GACGCGCACC ATGAGCGCAC CTTTATTGGC 
ATCTTCCTCA TTGATGGCGT CACTGGGCGT ATCATTCACT CCTCTGTGCA GAAGAAAGCC 
AAAGGCCCTG TCCATATCGT GCATTCAGAG AACTGGGTGG TGTACCAGTA CTGGAACACC 
AAGGCTCGGC GCAACGAGTT TACCGTACTG GAGCTCTATG AGGGCACTGA GCAATACAAC 
GCCACCGCCT TCAGCTCCCT GGACCGCCCC CAGCTGCCCC AGGTCCTCCA GCAGTCCTAT 
ATCTTCCCGT CCTCCATCAG TGCCATGGAG GCCACCATCA CCGAACGGGG CATCACCAGC 
CGACACCTGC TGATTGGACT ACCTTCTGGA GCAATTCTTT CCCTTCCTAA GGCTTTGCTG 
GATCCCCGCC GCCCCGAGAT CCCAACAGAA CAAAGCAGAG AGGAGAACTT AATCCCGTAT 
TCTCCAGATG TACAGATACA CGCAGAGCGA TTCATCAACT ATAACCAGAC AGTTTCTCGA 
ATGCGAGGTA TCTACACAGC TCCCTCGGGT CTGGAGTCCA CTTGTTTGGT TGTGGCCTAT 
GGTTTGGACA TTTACCAAAC TCGAGTCTAC CCATCCAAGC AGTTTGACGT TCTGAAGGAT 
GACTATGACT ACGTGTTAAT CAGCAGCGTC CTCTTTGGCC TGGTTTTTGC CACCATGATC 
ACTAAGAGAC TGGCACAGGT GAAGCTCCTG AATCGGGCCT GGCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005245845.1
CDS	46..3033
Translation	MAAEWASRFWLWATLLIPAAAVYEDQVGKFDWRQQYVGKVKFASLEFSPGSKKLVVATEKNVIAALNSRTGEILWRHVDKGTAEGAVDAMLLHGQDVITVSNGGRIMRSWETNIGGLNWEITLDSGSFQALGLVGLQESVRYIAVLKKTTLALHHLSSGHLKWVEHLPESDSIHYQMVYSYGSGVVWALGVVPFSHVNIVKFNVEDGEIVQQVRVSTPWLQHLSGACGVVDEAVLVCPDPSSRSLQTLALETEWELRQIPLQSLDLEFGSGFQPRVLPTQPNPVDASRAQFFLHLSPSHYALLQYHYGTLSLLKNFPQTALVSFATTGEKTVAAVMACRNEVKSSSSEDGSMGSFSEKSSSKDSLACFNQTYTINLYLVETGRRLLDTTITFSLEQSGTRPERLYIQVFLKKDDSVGYRALVQTEDHLLLFLQQLAGKVVLWSREESLAEVVCLEMVDLPLTGAQAELEGEFGKKAAIQDGLLGMFLKRLSSQLILLQAWTSHLWKMFYDARKPRSQIKNEINIDTLARDEFNLQKMMVMVTASGKLFGIESSSGTILWKQYLPNVKPDSSFKLMVQRTTAHFPHPPQCTLLVKDKESGMSSLYVFNPIFGKWSQVAPPVLKRPILQSLLLPVMDQDYAKVLLLIDDEYKVTAFPATRNVLRQLHELAPSIFFYLVDAEQGRLCGYRLRKDLTTELSWELTIPPEVQRIVKVKGKRSSEHVHSQGRVMGDRSVLYKSLNPNLLAVVTESTDAHHERTFIGIFLIDGVTGRIIHSSVQKKAKGPVHIVHSENWVVYQYWNTKARRNEFTVLELYEGTEQYNATAFSSLDRPQLPQVLQQSYIFPSSISAMEATITERGITSRHLLIGLPSGAILSLPKALLDPRRPEIPTEQSREENLIPYSPDVQIHAERFINYNQTVSRMRGIYTAPSGLESTCLVVAYGLDIYQTRVYPSKQFDVLKDDYDYVLISSVLFGLVFATMITKRLAQVKLLNRAWR

Target ORF information:

RefSeq Version	XM_005245788.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005245788.1

ORF Insert Sequence:

ATGGCGGCTG AGTGGGCTTC TCGTTTCTGG CTTTGGGCTA CGCTGCTGAT TCCTGCGGCC 
GCGGTCTACG AAGACCAAGT GGGCAAGTTT GATTGGAGAC AGCAATATGT TGGGAAGGTC 
AAGTTTGCCT CCTTGGAATT TTCCCCTGGA TCCAAGAAGT TGGTTGTAGC CACAGAGAAG 
AATGTGATTG CAGCATTAAA TTCCCGAACT GGGGAGATCT TGTGGCGCCA TGTTGACAAG 
GGCACGGCAG AAGGGGCTGT GGATGCCATG CTGCTGCACG GACAGGATGT GATCACTGTG 
TCCAATGGAG GCCGAATCAT GCGTTCCTGG GAGACTAACA TCGGGGGCCT GAACTGGGAG 
ATAACCCTGG ACAGTGGCAG TTTCCAGGCA CTTGGGCTGG TTGGCCTGCA GGAGTCTGTA 
AGGTACATCG CAGTCCTGAA GAAGACTACA CTTGCCCTCC ATCACCTCTC CAGTGGGCAC 
CTCAAGTGGG TGGAACATCT CCCAGAAAGT GACAGCATCC ACTACCAGAT GGTGTATTCT 
TACGGCTCTG GGGTGGTGTG GGCCCTCGGA GTTGTTCCCT TCAGCCATGT GAACATTGTC 
AAGTTTAATG TGGAAGATGG AGAGATTGTT CAGCAGGTTA GGGTTTCAAC TCCGTGGCTG 
CAGCACCTGT CTGGAGCCTG TGGTGTGGTG GATGAGGCTG TCCTGGTGTG TCCTGACCCG 
AGCTCACGTT CCCTCCAAAC TTTGGCTCTG GAGACGGAAT GGGAGTTGAG ACAGATCCCA 
CTGCAGTCTC TCGACTTAGA ATTTGGAAGT GGATTCCAAC CCCGGGTCCT GCCTACCCAG 
CCCAACCCAG TGGACGCTTC CCGGGCCCAG TTCTTCCTGC ACTTGTCCCC AAGCCACTAT 
GCTCTGCTGC AGTACCATTA TGGAACGCTG AGTTTGCTTA AAAACTTCCC ACAGACTGCC 
CTAGTGAGCT TTGCCACCAC TGGGGAGAAG ACGGTGGCTG CAGTCATGGC CTGTCGGAAT 
GAAGTGAAAA GTAGCAGTTC TGAAGATGGG TCAATGGGGA GCTTTTCGGA GAAGTCTAGT 
TCAAAGGACT CTCTGGCTTG CTTCAATCAG ACCTACACCA TTAACCTATA CCTCGTGGAG 
ACAGGTCGGC GGCTGCTGGA CACCACGATA ACATTTAGCC TGGAACAGAG CGGCACTCGG 
CCTGAGCGGC TGTATATCCA GGTGTTCTTG AAGAAGGATG ACTCAGTGGG CTACCGGGCT 
TTGGTGCAGA CAGAGGATCA TCTGCTACTT TTCCTGCAGC AGTTGGCAGG GAAGGTGGTG 
CTGTGGAGCC GTGAGGAGTC CCTGGCAGAA GTGGTGTGCC TAGAGATGGT GGACCTCCCC 
CTGACTGGGG CACAGGCCGA GCTGGAAGGA GAATTTGGCA AAAAGGCAGC AATTCAAGAT 
GGCTTGCTGG GGATGTTCCT GAAACGCCTC TCGTCTCAGC TTATCCTGCT GCAAGCATGG 
ACTTCCCACC TCTGGAAAAT GTTTTATGAT GCTCGGAAGC CCCGGAGTCA GATTAAGAAT 
GAGATCAACA TTGACACCCT GGCCAGAGAT GAATTCAACC TCCAGAAGAT GATGGTGATG 
GTAACAGCCT CAGGCAAGCT TTTTGGCATT GAGAGCAGCT CTGGCACCAT CCTGTGGAAA 
CAGTATCTAC CCAATGTCAA GCCAGACTCC TCCTTTAAAC TGATGGTCCA GAGAACTACT 
GCTCATTTCC CCCATCCCCC ACAGTGCACC CTGCTGGTGA AGGACAAGGA GTCGGGAATG 
AGTTCTCTGT ATGTCTTCAA TCCCATTTTT GGGAAGTGGA GTCAGGTAGC TCCCCCAGTG 
CTGAAGCGCC CCATCTTGCA GTCCTTGCTT CTCCCAGTCA TGGATCAAGA CTACGCCAAG 
GTGTTGCTGT TGATAGATGA TGAATACAAG GTCACAGCTT TTCCAGCCAC TCGGAATGTC 
TTGCGACAGC TACATGAGCT TGCCCCTTCC ATCTTCTTCT ATTTGGTGGA TGCAGAGCAG 
GGACGGCTGT GTGGATATCG GCTTCGAAAG GATCTCACCA CTGAGCTGAG TTGGGAGCTG 
ACCATTCCCC CAGAAGTACA GCGGATCGTC AAGGTGAAGG GGAAACGCAG CAGTGAGCAC 
GTTCATTCCC AGGGCCGTGT GATGGGGGAC CGCAGTGTGC TCTACAAGAG CCTGAACCCC 
AACCTGCTGG CCGTGGTGAC AGAGAGCACA GACGCGCACC ATGAGCGCAC CTTTATTGGC 
ATCTTCCTCA TTGATGGCGT CACTGGGCGT ATCATTCACT CCTCTGTGCA GAAGAAAGCC 
AAAGGCCCTG TCCATATCGT GCATTCAGAG AACTGGGTGG TGTACCAGTA CTGGAACACC 
AAGGCTCGGC GCAACGAGTT TACCGTACTG GAGCTCTATG AGGGCACTGA GCAATACAAC 
GCCACCGCCT TCAGCTCCCT GGACCGCCCC CAGCTGCCCC AGGTCCTCCA GCAGTCCTAT 
ATCTTCCCGT CCTCCATCAG TGCCATGGAG GCCACCATCA CCGAACGGGG CATCACCAGC 
CGACACCTGC TGATTGGACT ACCTTCTGGA GCAATTCTTT CCCTTCCTAA GGCTTTGCTG 
GATCCCCGCC GCCCCGAGAT CCCAACAGAA CAAAGCAGAG AGGAGAACTT AATCCCGTAT 
TCTCCAGATG TACAGATACA CGCAGAGCGA TTCATCAACT ATAACCAGAC AGTTTCTCGA 
ATGCGAGGTA TCTACACAGC TCCCTCGGGT CTGGAGTCCA CTTGTTTGGT TGTGGCCTAT 
GGTTTGGACA TTTACCAAAC TCGAGTCTAC CCATCCAAGC AGTTTGACGT TCTGAAGGAT 
GACTATGACT ACGTGTTAAT CAGCAGCGTC CTCTTTGGCC TGGTTTTTGC CACCATGATC 
ACTAAGAGAC TGGCACAGGT GAAGCTCCTG AATCGGGCCT GGCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMC1 ( XM_005245788.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005245845.1 Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant X2, mRNA.

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CloneID	OHu33201
Clone ID Related Accession (Same CDS sequence)	NM_001375821.1 , XM_005245788.1 , XM_005245788.2
Accession Version	XM_005245788.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2988bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	ER membrane protein complex subunit 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005245788.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGGCGGCTG AGTGGGCTTC TCGTTTCTGG CTTTGGGCTA CGCTGCTGAT TCCTGCGGCC 
GCGGTCTACG AAGACCAAGT GGGCAAGTTT GATTGGAGAC AGCAATATGT TGGGAAGGTC 
AAGTTTGCCT CCTTGGAATT TTCCCCTGGA TCCAAGAAGT TGGTTGTAGC CACAGAGAAG 
AATGTGATTG CAGCATTAAA TTCCCGAACT GGGGAGATCT TGTGGCGCCA TGTTGACAAG 
GGCACGGCAG AAGGGGCTGT GGATGCCATG CTGCTGCACG GACAGGATGT GATCACTGTG 
TCCAATGGAG GCCGAATCAT GCGTTCCTGG GAGACTAACA TCGGGGGCCT GAACTGGGAG 
ATAACCCTGG ACAGTGGCAG TTTCCAGGCA CTTGGGCTGG TTGGCCTGCA GGAGTCTGTA 
AGGTACATCG CAGTCCTGAA GAAGACTACA CTTGCCCTCC ATCACCTCTC CAGTGGGCAC 
CTCAAGTGGG TGGAACATCT CCCAGAAAGT GACAGCATCC ACTACCAGAT GGTGTATTCT 
TACGGCTCTG GGGTGGTGTG GGCCCTCGGA GTTGTTCCCT TCAGCCATGT GAACATTGTC 
AAGTTTAATG TGGAAGATGG AGAGATTGTT CAGCAGGTTA GGGTTTCAAC TCCGTGGCTG 
CAGCACCTGT CTGGAGCCTG TGGTGTGGTG GATGAGGCTG TCCTGGTGTG TCCTGACCCG 
AGCTCACGTT CCCTCCAAAC TTTGGCTCTG GAGACGGAAT GGGAGTTGAG ACAGATCCCA 
CTGCAGTCTC TCGACTTAGA ATTTGGAAGT GGATTCCAAC CCCGGGTCCT GCCTACCCAG 
CCCAACCCAG TGGACGCTTC CCGGGCCCAG TTCTTCCTGC ACTTGTCCCC AAGCCACTAT 
GCTCTGCTGC AGTACCATTA TGGAACGCTG AGTTTGCTTA AAAACTTCCC ACAGACTGCC 
CTAGTGAGCT TTGCCACCAC TGGGGAGAAG ACGGTGGCTG CAGTCATGGC CTGTCGGAAT 
GAAGTGAAAA GTAGCAGTTC TGAAGATGGG TCAATGGGGA GCTTTTCGGA GAAGTCTAGT 
TCAAAGGACT CTCTGGCTTG CTTCAATCAG ACCTACACCA TTAACCTATA CCTCGTGGAG 
ACAGGTCGGC GGCTGCTGGA CACCACGATA ACATTTAGCC TGGAACAGAG CGGCACTCGG 
CCTGAGCGGC TGTATATCCA GGTGTTCTTG AAGAAGGATG ACTCAGTGGG CTACCGGGCT 
TTGGTGCAGA CAGAGGATCA TCTGCTACTT TTCCTGCAGC AGTTGGCAGG GAAGGTGGTG 
CTGTGGAGCC GTGAGGAGTC CCTGGCAGAA GTGGTGTGCC TAGAGATGGT GGACCTCCCC 
CTGACTGGGG CACAGGCCGA GCTGGAAGGA GAATTTGGCA AAAAGGCAGC AATTCAAGAT 
GGCTTGCTGG GGATGTTCCT GAAACGCCTC TCGTCTCAGC TTATCCTGCT GCAAGCATGG 
ACTTCCCACC TCTGGAAAAT GTTTTATGAT GCTCGGAAGC CCCGGAGTCA GATTAAGAAT 
GAGATCAACA TTGACACCCT GGCCAGAGAT GAATTCAACC TCCAGAAGAT GATGGTGATG 
GTAACAGCCT CAGGCAAGCT TTTTGGCATT GAGAGCAGCT CTGGCACCAT CCTGTGGAAA 
CAGTATCTAC CCAATGTCAA GCCAGACTCC TCCTTTAAAC TGATGGTCCA GAGAACTACT 
GCTCATTTCC CCCATCCCCC ACAGTGCACC CTGCTGGTGA AGGACAAGGA GTCGGGAATG 
AGTTCTCTGT ATGTCTTCAA TCCCATTTTT GGGAAGTGGA GTCAGGTAGC TCCCCCAGTG 
CTGAAGCGCC CCATCTTGCA GTCCTTGCTT CTCCCAGTCA TGGATCAAGA CTACGCCAAG 
GTGTTGCTGT TGATAGATGA TGAATACAAG GTCACAGCTT TTCCAGCCAC TCGGAATGTC 
TTGCGACAGC TACATGAGCT TGCCCCTTCC ATCTTCTTCT ATTTGGTGGA TGCAGAGCAG 
GGACGGCTGT GTGGATATCG GCTTCGAAAG GATCTCACCA CTGAGCTGAG TTGGGAGCTG 
ACCATTCCCC CAGAAGTACA GCGGATCGTC AAGGTGAAGG GGAAACGCAG CAGTGAGCAC 
GTTCATTCCC AGGGCCGTGT GATGGGGGAC CGCAGTGTGC TCTACAAGAG CCTGAACCCC 
AACCTGCTGG CCGTGGTGAC AGAGAGCACA GACGCGCACC ATGAGCGCAC CTTTATTGGC 
ATCTTCCTCA TTGATGGCGT CACTGGGCGT ATCATTCACT CCTCTGTGCA GAAGAAAGCC 
AAAGGCCCTG TCCATATCGT GCATTCAGAG AACTGGGTGG TGTACCAGTA CTGGAACACC 
AAGGCTCGGC GCAACGAGTT TACCGTACTG GAGCTCTATG AGGGCACTGA GCAATACAAC 
GCCACCGCCT TCAGCTCCCT GGACCGCCCC CAGCTGCCCC AGGTCCTCCA GCAGTCCTAT 
ATCTTCCCGT CCTCCATCAG TGCCATGGAG GCCACCATCA CCGAACGGGG CATCACCAGC 
CGACACCTGC TGATTGGACT ACCTTCTGGA GCAATTCTTT CCCTTCCTAA GGCTTTGCTG 
GATCCCCGCC GCCCCGAGAT CCCAACAGAA CAAAGCAGAG AGGAGAACTT AATCCCGTAT 
TCTCCAGATG TACAGATACA CGCAGAGCGA TTCATCAACT ATAACCAGAC AGTTTCTCGA 
ATGCGAGGTA TCTACACAGC TCCCTCGGGT CTGGAGTCCA CTTGTTTGGT TGTGGCCTAT 
GGTTTGGACA TTTACCAAAC TCGAGTCTAC CCATCCAAGC AGTTTGACGT TCTGAAGGAT 
GACTATGACT ACGTGTTAAT CAGCAGCGTC CTCTTTGGCC TGGTTTTTGC CACCATGATC 
ACTAAGAGAC TGGCACAGGT GAAGCTCCTG AATCGGGCCT GGCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005245845.1
CDS	39..3026
Translation	MAAEWASRFWLWATLLIPAAAVYEDQVGKFDWRQQYVGKVKFASLEFSPGSKKLVVATEKNVIAALNSRTGEILWRHVDKGTAEGAVDAMLLHGQDVITVSNGGRIMRSWETNIGGLNWEITLDSGSFQALGLVGLQESVRYIAVLKKTTLALHHLSSGHLKWVEHLPESDSIHYQMVYSYGSGVVWALGVVPFSHVNIVKFNVEDGEIVQQVRVSTPWLQHLSGACGVVDEAVLVCPDPSSRSLQTLALETEWELRQIPLQSLDLEFGSGFQPRVLPTQPNPVDASRAQFFLHLSPSHYALLQYHYGTLSLLKNFPQTALVSFATTGEKTVAAVMACRNEVKSSSSEDGSMGSFSEKSSSKDSLACFNQTYTINLYLVETGRRLLDTTITFSLEQSGTRPERLYIQVFLKKDDSVGYRALVQTEDHLLLFLQQLAGKVVLWSREESLAEVVCLEMVDLPLTGAQAELEGEFGKKAAIQDGLLGMFLKRLSSQLILLQAWTSHLWKMFYDARKPRSQIKNEINIDTLARDEFNLQKMMVMVTASGKLFGIESSSGTILWKQYLPNVKPDSSFKLMVQRTTAHFPHPPQCTLLVKDKESGMSSLYVFNPIFGKWSQVAPPVLKRPILQSLLLPVMDQDYAKVLLLIDDEYKVTAFPATRNVLRQLHELAPSIFFYLVDAEQGRLCGYRLRKDLTTELSWELTIPPEVQRIVKVKGKRSSEHVHSQGRVMGDRSVLYKSLNPNLLAVVTESTDAHHERTFIGIFLIDGVTGRIIHSSVQKKAKGPVHIVHSENWVVYQYWNTKARRNEFTVLELYEGTEQYNATAFSSLDRPQLPQVLQQSYIFPSSISAMEATITERGITSRHLLIGLPSGAILSLPKALLDPRRPEIPTEQSREENLIPYSPDVQIHAERFINYNQTVSRMRGIYTAPSGLESTCLVVAYGLDIYQTRVYPSKQFDVLKDDYDYVLISSVLFGLVFATMITKRLAQVKLLNRAWR

Target ORF information:

RefSeq Version	XM_005245788.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005245788.2

ORF Insert Sequence:

ATGGCGGCTG AGTGGGCTTC TCGTTTCTGG CTTTGGGCTA CGCTGCTGAT TCCTGCGGCC 
GCGGTCTACG AAGACCAAGT GGGCAAGTTT GATTGGAGAC AGCAATATGT TGGGAAGGTC 
AAGTTTGCCT CCTTGGAATT TTCCCCTGGA TCCAAGAAGT TGGTTGTAGC CACAGAGAAG 
AATGTGATTG CAGCATTAAA TTCCCGAACT GGGGAGATCT TGTGGCGCCA TGTTGACAAG 
GGCACGGCAG AAGGGGCTGT GGATGCCATG CTGCTGCACG GACAGGATGT GATCACTGTG 
TCCAATGGAG GCCGAATCAT GCGTTCCTGG GAGACTAACA TCGGGGGCCT GAACTGGGAG 
ATAACCCTGG ACAGTGGCAG TTTCCAGGCA CTTGGGCTGG TTGGCCTGCA GGAGTCTGTA 
AGGTACATCG CAGTCCTGAA GAAGACTACA CTTGCCCTCC ATCACCTCTC CAGTGGGCAC 
CTCAAGTGGG TGGAACATCT CCCAGAAAGT GACAGCATCC ACTACCAGAT GGTGTATTCT 
TACGGCTCTG GGGTGGTGTG GGCCCTCGGA GTTGTTCCCT TCAGCCATGT GAACATTGTC 
AAGTTTAATG TGGAAGATGG AGAGATTGTT CAGCAGGTTA GGGTTTCAAC TCCGTGGCTG 
CAGCACCTGT CTGGAGCCTG TGGTGTGGTG GATGAGGCTG TCCTGGTGTG TCCTGACCCG 
AGCTCACGTT CCCTCCAAAC TTTGGCTCTG GAGACGGAAT GGGAGTTGAG ACAGATCCCA 
CTGCAGTCTC TCGACTTAGA ATTTGGAAGT GGATTCCAAC CCCGGGTCCT GCCTACCCAG 
CCCAACCCAG TGGACGCTTC CCGGGCCCAG TTCTTCCTGC ACTTGTCCCC AAGCCACTAT 
GCTCTGCTGC AGTACCATTA TGGAACGCTG AGTTTGCTTA AAAACTTCCC ACAGACTGCC 
CTAGTGAGCT TTGCCACCAC TGGGGAGAAG ACGGTGGCTG CAGTCATGGC CTGTCGGAAT 
GAAGTGAAAA GTAGCAGTTC TGAAGATGGG TCAATGGGGA GCTTTTCGGA GAAGTCTAGT 
TCAAAGGACT CTCTGGCTTG CTTCAATCAG ACCTACACCA TTAACCTATA CCTCGTGGAG 
ACAGGTCGGC GGCTGCTGGA CACCACGATA ACATTTAGCC TGGAACAGAG CGGCACTCGG 
CCTGAGCGGC TGTATATCCA GGTGTTCTTG AAGAAGGATG ACTCAGTGGG CTACCGGGCT 
TTGGTGCAGA CAGAGGATCA TCTGCTACTT TTCCTGCAGC AGTTGGCAGG GAAGGTGGTG 
CTGTGGAGCC GTGAGGAGTC CCTGGCAGAA GTGGTGTGCC TAGAGATGGT GGACCTCCCC 
CTGACTGGGG CACAGGCCGA GCTGGAAGGA GAATTTGGCA AAAAGGCAGC AATTCAAGAT 
GGCTTGCTGG GGATGTTCCT GAAACGCCTC TCGTCTCAGC TTATCCTGCT GCAAGCATGG 
ACTTCCCACC TCTGGAAAAT GTTTTATGAT GCTCGGAAGC CCCGGAGTCA GATTAAGAAT 
GAGATCAACA TTGACACCCT GGCCAGAGAT GAATTCAACC TCCAGAAGAT GATGGTGATG 
GTAACAGCCT CAGGCAAGCT TTTTGGCATT GAGAGCAGCT CTGGCACCAT CCTGTGGAAA 
CAGTATCTAC CCAATGTCAA GCCAGACTCC TCCTTTAAAC TGATGGTCCA GAGAACTACT 
GCTCATTTCC CCCATCCCCC ACAGTGCACC CTGCTGGTGA AGGACAAGGA GTCGGGAATG 
AGTTCTCTGT ATGTCTTCAA TCCCATTTTT GGGAAGTGGA GTCAGGTAGC TCCCCCAGTG 
CTGAAGCGCC CCATCTTGCA GTCCTTGCTT CTCCCAGTCA TGGATCAAGA CTACGCCAAG 
GTGTTGCTGT TGATAGATGA TGAATACAAG GTCACAGCTT TTCCAGCCAC TCGGAATGTC 
TTGCGACAGC TACATGAGCT TGCCCCTTCC ATCTTCTTCT ATTTGGTGGA TGCAGAGCAG 
GGACGGCTGT GTGGATATCG GCTTCGAAAG GATCTCACCA CTGAGCTGAG TTGGGAGCTG 
ACCATTCCCC CAGAAGTACA GCGGATCGTC AAGGTGAAGG GGAAACGCAG CAGTGAGCAC 
GTTCATTCCC AGGGCCGTGT GATGGGGGAC CGCAGTGTGC TCTACAAGAG CCTGAACCCC 
AACCTGCTGG CCGTGGTGAC AGAGAGCACA GACGCGCACC ATGAGCGCAC CTTTATTGGC 
ATCTTCCTCA TTGATGGCGT CACTGGGCGT ATCATTCACT CCTCTGTGCA GAAGAAAGCC 
AAAGGCCCTG TCCATATCGT GCATTCAGAG AACTGGGTGG TGTACCAGTA CTGGAACACC 
AAGGCTCGGC GCAACGAGTT TACCGTACTG GAGCTCTATG AGGGCACTGA GCAATACAAC 
GCCACCGCCT TCAGCTCCCT GGACCGCCCC CAGCTGCCCC AGGTCCTCCA GCAGTCCTAT 
ATCTTCCCGT CCTCCATCAG TGCCATGGAG GCCACCATCA CCGAACGGGG CATCACCAGC 
CGACACCTGC TGATTGGACT ACCTTCTGGA GCAATTCTTT CCCTTCCTAA GGCTTTGCTG 
GATCCCCGCC GCCCCGAGAT CCCAACAGAA CAAAGCAGAG AGGAGAACTT AATCCCGTAT 
TCTCCAGATG TACAGATACA CGCAGAGCGA TTCATCAACT ATAACCAGAC AGTTTCTCGA 
ATGCGAGGTA TCTACACAGC TCCCTCGGGT CTGGAGTCCA CTTGTTTGGT TGTGGCCTAT 
GGTTTGGACA TTTACCAAAC TCGAGTCTAC CCATCCAAGC AGTTTGACGT TCTGAAGGAT 
GACTATGACT ACGTGTTAAT CAGCAGCGTC CTCTTTGGCC TGGTTTTTGC CACCATGATC 
ACTAAGAGAC TGGCACAGGT GAAGCTCCTG AATCGGGCCT GGCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMC1 ( NM_001375820.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001362749.1 Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu33200
Clone ID Related Accession (Same CDS sequence)	XM_005245787.1 , NM_001375820.1 , XM_005245787.2
Accession Version	NM_001375820.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2991bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	ER membrane protein complex subunit 1 isoform 5 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL035413.19 and AL357564.17. On Nov 5, 2019 this sequence version replaced XM_005245787.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.195330.1, SRR1803614.84516.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCGGCTG AGTGGGCTTC TCGTTTCTGG CTTTGGGCTA CGCTGCTGAT TCCTGCGGCC 
GCGGTCTACG AAGACCAAGT GGGCAAGTTT GATTGGAGAC AGCAATATGT TGGGAAGGTC 
AAGTTTGCCT CCTTGGAATT TTCCCCTGGA TCCAAGAAGT TGGTTGTAGC CACAGAGAAG 
AATGTGATTG CAGCATTAAA TTCCCGAACT GGGGAGATCT TGTGGCGCCA TGTTGACAAG 
GGCACGGCAG AAGGGGCTGT GGATGCCATG CTGCTGCACG GACAGGATGT GATCACTGTG 
TCCAATGGAG GCCGAATCAT GCGTTCCTGG GAGACTAACA TCGGGGGCCT GAACTGGGAG 
ATAACCCTGG ACAGTGGCAG TTTCCAGGCA CTTGGGCTGG TTGGCCTGCA GGAGTCTGTA 
AGGTACATCG CAGTCCTGAA GAAGACTACA CTTGCCCTCC ATCACCTCTC CAGTGGGCAC 
CTCAAGTGGG TGGAACATCT CCCAGAAAGT GACAGCATCC ACTACCAGAT GGTGTATTCT 
TACGGCTCTG GGGTGGTGTG GGCCCTCGGA GTTGTTCCCT TCAGCCATGT GAACATTGTC 
AAGTTTAATG TGGAAGATGG AGAGATTGTT CAGCAGGTTA GGGTTTCAAC TCCGTGGCTG 
CAGCACCTGT CTGGAGCCTG TGGTGTGGTG GATGAGGCTG TCCTGGTGTG TCCTGACCCG 
AGCTCACGTT CCCTCCAAAC TTTGGCTCTG GAGACGGAAT GGGAGTTGAG ACAGATCCCA 
CTGCAGTCTC TCGACTTAGA ATTTGGAAGT GGATTCCAAC CCCGGGTCCT GCCTACCCAG 
CCCAACCCAG TGGACGCTTC CCGGGCCCAG TTCTTCCTGC ACTTGTCCCC AAGCCACTAT 
GCTCTGCTGC AGTACCATTA TGGAACGCTG AGTTTGCTTA AAAACTTCCC ACAGACTGCC 
CTAGTGAGCT TTGCCACCAC TGGGGAGAAG ACGGTGGCTG CAGTCATGGC CTGTCGGAAT 
GAAGTGCAGA AAAGTAGCAG TTCTGAAGAT GGGTCAATGG GGAGCTTTTC GGAGAAGTCT 
AGTTCAAAGG ACTCTCTGGC TTGCTTCAAT CAGACCTACA CCATTAACCT ATACCTCGTG 
GAGACAGGTC GGCGGCTGCT GGACACCACG ATAACATTTA GCCTGGAACA GAGCGGCACT 
CGGCCTGAGC GGCTGTATAT CCAGGTGTTC TTGAAGAAGG ATGACTCAGT GGGCTACCGG 
GCTTTGGTGC AGACAGAGGA TCATCTGCTA CTTTTCCTGC AGCAGTTGGC AGGGAAGGTG 
GTGCTGTGGA GCCGTGAGGA GTCCCTGGCA GAAGTGGTGT GCCTAGAGAT GGTGGACCTC 
CCCCTGACTG GGGCACAGGC CGAGCTGGAA GGAGAATTTG GCAAAAAGGC AGCAATTCAA 
GATGGCTTGC TGGGGATGTT CCTGAAACGC CTCTCGTCTC AGCTTATCCT GCTGCAAGCA 
TGGACTTCCC ACCTCTGGAA AATGTTTTAT GATGCTCGGA AGCCCCGGAG TCAGATTAAG 
AATGAGATCA ACATTGACAC CCTGGCCAGA GATGAATTCA ACCTCCAGAA GATGATGGTG 
ATGGTAACAG CCTCAGGCAA GCTTTTTGGC ATTGAGAGCA GCTCTGGCAC CATCCTGTGG 
AAACAGTATC TACCCAATGT CAAGCCAGAC TCCTCCTTTA AACTGATGGT CCAGAGAACT 
ACTGCTCATT TCCCCCATCC CCCACAGTGC ACCCTGCTGG TGAAGGACAA GGAGTCGGGA 
ATGAGTTCTC TGTATGTCTT CAATCCCATT TTTGGGAAGT GGAGTCAGGT AGCTCCCCCA 
GTGCTGAAGC GCCCCATCTT GCAGTCCTTG CTTCTCCCAG TCATGGATCA AGACTACGCC 
AAGGTGTTGC TGTTGATAGA TGATGAATAC AAGGTCACAG CTTTTCCAGC CACTCGGAAT 
GTCTTGCGAC AGCTACATGA GCTTGCCCCT TCCATCTTCT TCTATTTGGT GGATGCAGAG 
CAGGGACGGC TGTGTGGATA TCGGCTTCGA AAGGATCTCA CCACTGAGCT GAGTTGGGAG 
CTGACCATTC CCCCAGAAGT ACAGCGGATC GTCAAGGTGA AGGGGAAACG CAGCAGTGAG 
CACGTTCATT CCCAGGGCCG TGTGATGGGG GACCGCAGTG TGCTCTACAA GAGCCTGAAC 
CCCAACCTGC TGGCCGTGGT GACAGAGAGC ACAGACGCGC ACCATGAGCG CACCTTTATT 
GGCATCTTCC TCATTGATGG CGTCACTGGG CGTATCATTC ACTCCTCTGT GCAGAAGAAA 
GCCAAAGGCC CTGTCCATAT CGTGCATTCA GAGAACTGGG TGGTGTACCA GTACTGGAAC 
ACCAAGGCTC GGCGCAACGA GTTTACCGTA CTGGAGCTCT ATGAGGGCAC TGAGCAATAC 
AACGCCACCG CCTTCAGCTC CCTGGACCGC CCCCAGCTGC CCCAGGTCCT CCAGCAGTCC 
TATATCTTCC CGTCCTCCAT CAGTGCCATG GAGGCCACCA TCACCGAACG GGGCATCACC 
AGCCGACACC TGCTGATTGG ACTACCTTCT GGAGCAATTC TTTCCCTTCC TAAGGCTTTG 
CTGGATCCCC GCCGCCCCGA GATCCCAACA GAACAAAGCA GAGAGGAGAA CTTAATCCCG 
TATTCTCCAG ATGTACAGAT ACACGCAGAG CGATTCATCA ACTATAACCA GACAGTTTCT 
CGAATGCGAG GTATCTACAC AGCTCCCTCG GGTCTGGAGT CCACTTGTTT GGTTGTGGCC 
TATGGTTTGG ACATTTACCA AACTCGAGTC TACCCATCCA AGCAGTTTGA CGTTCTGAAG 
GATGACTATG ACTACGTGTT AATCAGCAGC GTCCTCTTTG GCCTGGTTTT TGCCACCATG 
ATCACTAAGA GACTGGCACA GGTGAAGCTC CTGAATCGGG CCTGGCGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001362749.1
CDS	16..3006
Translation	MAAEWASRFWLWATLLIPAAAVYEDQVGKFDWRQQYVGKVKFASLEFSPGSKKLVVATEKNVIAALNSRTGEILWRHVDKGTAEGAVDAMLLHGQDVITVSNGGRIMRSWETNIGGLNWEITLDSGSFQALGLVGLQESVRYIAVLKKTTLALHHLSSGHLKWVEHLPESDSIHYQMVYSYGSGVVWALGVVPFSHVNIVKFNVEDGEIVQQVRVSTPWLQHLSGACGVVDEAVLVCPDPSSRSLQTLALETEWELRQIPLQSLDLEFGSGFQPRVLPTQPNPVDASRAQFFLHLSPSHYALLQYHYGTLSLLKNFPQTALVSFATTGEKTVAAVMACRNEVQKSSSSEDGSMGSFSEKSSSKDSLACFNQTYTINLYLVETGRRLLDTTITFSLEQSGTRPERLYIQVFLKKDDSVGYRALVQTEDHLLLFLQQLAGKVVLWSREESLAEVVCLEMVDLPLTGAQAELEGEFGKKAAIQDGLLGMFLKRLSSQLILLQAWTSHLWKMFYDARKPRSQIKNEINIDTLARDEFNLQKMMVMVTASGKLFGIESSSGTILWKQYLPNVKPDSSFKLMVQRTTAHFPHPPQCTLLVKDKESGMSSLYVFNPIFGKWSQVAPPVLKRPILQSLLLPVMDQDYAKVLLLIDDEYKVTAFPATRNVLRQLHELAPSIFFYLVDAEQGRLCGYRLRKDLTTELSWELTIPPEVQRIVKVKGKRSSEHVHSQGRVMGDRSVLYKSLNPNLLAVVTESTDAHHERTFIGIFLIDGVTGRIIHSSVQKKAKGPVHIVHSENWVVYQYWNTKARRNEFTVLELYEGTEQYNATAFSSLDRPQLPQVLQQSYIFPSSISAMEATITERGITSRHLLIGLPSGAILSLPKALLDPRRPEIPTEQSREENLIPYSPDVQIHAERFINYNQTVSRMRGIYTAPSGLESTCLVVAYGLDIYQTRVYPSKQFDVLKDDYDYVLISSVLFGLVFATMITKRLAQVKLLNRAWR

Target ORF information:

RefSeq Version	NM_001375820.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001375820.1

ORF Insert Sequence:

ATGGCGGCTG AGTGGGCTTC TCGTTTCTGG CTTTGGGCTA CGCTGCTGAT TCCTGCGGCC 
GCGGTCTACG AAGACCAAGT GGGCAAGTTT GATTGGAGAC AGCAATATGT TGGGAAGGTC 
AAGTTTGCCT CCTTGGAATT TTCCCCTGGA TCCAAGAAGT TGGTTGTAGC CACAGAGAAG 
AATGTGATTG CAGCATTAAA TTCCCGAACT GGGGAGATCT TGTGGCGCCA TGTTGACAAG 
GGCACGGCAG AAGGGGCTGT GGATGCCATG CTGCTGCACG GACAGGATGT GATCACTGTG 
TCCAATGGAG GCCGAATCAT GCGTTCCTGG GAGACTAACA TCGGGGGCCT GAACTGGGAG 
ATAACCCTGG ACAGTGGCAG TTTCCAGGCA CTTGGGCTGG TTGGCCTGCA GGAGTCTGTA 
AGGTACATCG CAGTCCTGAA GAAGACTACA CTTGCCCTCC ATCACCTCTC CAGTGGGCAC 
CTCAAGTGGG TGGAACATCT CCCAGAAAGT GACAGCATCC ACTACCAGAT GGTGTATTCT 
TACGGCTCTG GGGTGGTGTG GGCCCTCGGA GTTGTTCCCT TCAGCCATGT GAACATTGTC 
AAGTTTAATG TGGAAGATGG AGAGATTGTT CAGCAGGTTA GGGTTTCAAC TCCGTGGCTG 
CAGCACCTGT CTGGAGCCTG TGGTGTGGTG GATGAGGCTG TCCTGGTGTG TCCTGACCCG 
AGCTCACGTT CCCTCCAAAC TTTGGCTCTG GAGACGGAAT GGGAGTTGAG ACAGATCCCA 
CTGCAGTCTC TCGACTTAGA ATTTGGAAGT GGATTCCAAC CCCGGGTCCT GCCTACCCAG 
CCCAACCCAG TGGACGCTTC CCGGGCCCAG TTCTTCCTGC ACTTGTCCCC AAGCCACTAT 
GCTCTGCTGC AGTACCATTA TGGAACGCTG AGTTTGCTTA AAAACTTCCC ACAGACTGCC 
CTAGTGAGCT TTGCCACCAC TGGGGAGAAG ACGGTGGCTG CAGTCATGGC CTGTCGGAAT 
GAAGTGCAGA AAAGTAGCAG TTCTGAAGAT GGGTCAATGG GGAGCTTTTC GGAGAAGTCT 
AGTTCAAAGG ACTCTCTGGC TTGCTTCAAT CAGACCTACA CCATTAACCT ATACCTCGTG 
GAGACAGGTC GGCGGCTGCT GGACACCACG ATAACATTTA GCCTGGAACA GAGCGGCACT 
CGGCCTGAGC GGCTGTATAT CCAGGTGTTC TTGAAGAAGG ATGACTCAGT GGGCTACCGG 
GCTTTGGTGC AGACAGAGGA TCATCTGCTA CTTTTCCTGC AGCAGTTGGC AGGGAAGGTG 
GTGCTGTGGA GCCGTGAGGA GTCCCTGGCA GAAGTGGTGT GCCTAGAGAT GGTGGACCTC 
CCCCTGACTG GGGCACAGGC CGAGCTGGAA GGAGAATTTG GCAAAAAGGC AGCAATTCAA 
GATGGCTTGC TGGGGATGTT CCTGAAACGC CTCTCGTCTC AGCTTATCCT GCTGCAAGCA 
TGGACTTCCC ACCTCTGGAA AATGTTTTAT GATGCTCGGA AGCCCCGGAG TCAGATTAAG 
AATGAGATCA ACATTGACAC CCTGGCCAGA GATGAATTCA ACCTCCAGAA GATGATGGTG 
ATGGTAACAG CCTCAGGCAA GCTTTTTGGC ATTGAGAGCA GCTCTGGCAC CATCCTGTGG 
AAACAGTATC TACCCAATGT CAAGCCAGAC TCCTCCTTTA AACTGATGGT CCAGAGAACT 
ACTGCTCATT TCCCCCATCC CCCACAGTGC ACCCTGCTGG TGAAGGACAA GGAGTCGGGA 
ATGAGTTCTC TGTATGTCTT CAATCCCATT TTTGGGAAGT GGAGTCAGGT AGCTCCCCCA 
GTGCTGAAGC GCCCCATCTT GCAGTCCTTG CTTCTCCCAG TCATGGATCA AGACTACGCC 
AAGGTGTTGC TGTTGATAGA TGATGAATAC AAGGTCACAG CTTTTCCAGC CACTCGGAAT 
GTCTTGCGAC AGCTACATGA GCTTGCCCCT TCCATCTTCT TCTATTTGGT GGATGCAGAG 
CAGGGACGGC TGTGTGGATA TCGGCTTCGA AAGGATCTCA CCACTGAGCT GAGTTGGGAG 
CTGACCATTC CCCCAGAAGT ACAGCGGATC GTCAAGGTGA AGGGGAAACG CAGCAGTGAG 
CACGTTCATT CCCAGGGCCG TGTGATGGGG GACCGCAGTG TGCTCTACAA GAGCCTGAAC 
CCCAACCTGC TGGCCGTGGT GACAGAGAGC ACAGACGCGC ACCATGAGCG CACCTTTATT 
GGCATCTTCC TCATTGATGG CGTCACTGGG CGTATCATTC ACTCCTCTGT GCAGAAGAAA 
GCCAAAGGCC CTGTCCATAT CGTGCATTCA GAGAACTGGG TGGTGTACCA GTACTGGAAC 
ACCAAGGCTC GGCGCAACGA GTTTACCGTA CTGGAGCTCT ATGAGGGCAC TGAGCAATAC 
AACGCCACCG CCTTCAGCTC CCTGGACCGC CCCCAGCTGC CCCAGGTCCT CCAGCAGTCC 
TATATCTTCC CGTCCTCCAT CAGTGCCATG GAGGCCACCA TCACCGAACG GGGCATCACC 
AGCCGACACC TGCTGATTGG ACTACCTTCT GGAGCAATTC TTTCCCTTCC TAAGGCTTTG 
CTGGATCCCC GCCGCCCCGA GATCCCAACA GAACAAAGCA GAGAGGAGAA CTTAATCCCG 
TATTCTCCAG ATGTACAGAT ACACGCAGAG CGATTCATCA ACTATAACCA GACAGTTTCT 
CGAATGCGAG GTATCTACAC AGCTCCCTCG GGTCTGGAGT CCACTTGTTT GGTTGTGGCC 
TATGGTTTGG ACATTTACCA AACTCGAGTC TACCCATCCA AGCAGTTTGA CGTTCTGAAG 
GATGACTATG ACTACGTGTT AATCAGCAGC GTCCTCTTTG GCCTGGTTTT TGCCACCATG 
ATCACTAAGA GACTGGCACA GGTGAAGCTC CTGAATCGGG CCTGGCGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMC1 ( NM_001375821.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001362750.1 Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant 6, mRNA.

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CloneID	OHu33201
Clone ID Related Accession (Same CDS sequence)	NM_001375821.1 , XM_005245788.1 , XM_005245788.2
Accession Version	NM_001375821.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2988bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	ER membrane protein complex subunit 1 isoform 6 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL035413.19 and AL357564.17. On Nov 5, 2019 this sequence version replaced XM_005245788.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.27585.1, SRR1660807.70187.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCGGCTG AGTGGGCTTC TCGTTTCTGG CTTTGGGCTA CGCTGCTGAT TCCTGCGGCC 
GCGGTCTACG AAGACCAAGT GGGCAAGTTT GATTGGAGAC AGCAATATGT TGGGAAGGTC 
AAGTTTGCCT CCTTGGAATT TTCCCCTGGA TCCAAGAAGT TGGTTGTAGC CACAGAGAAG 
AATGTGATTG CAGCATTAAA TTCCCGAACT GGGGAGATCT TGTGGCGCCA TGTTGACAAG 
GGCACGGCAG AAGGGGCTGT GGATGCCATG CTGCTGCACG GACAGGATGT GATCACTGTG 
TCCAATGGAG GCCGAATCAT GCGTTCCTGG GAGACTAACA TCGGGGGCCT GAACTGGGAG 
ATAACCCTGG ACAGTGGCAG TTTCCAGGCA CTTGGGCTGG TTGGCCTGCA GGAGTCTGTA 
AGGTACATCG CAGTCCTGAA GAAGACTACA CTTGCCCTCC ATCACCTCTC CAGTGGGCAC 
CTCAAGTGGG TGGAACATCT CCCAGAAAGT GACAGCATCC ACTACCAGAT GGTGTATTCT 
TACGGCTCTG GGGTGGTGTG GGCCCTCGGA GTTGTTCCCT TCAGCCATGT GAACATTGTC 
AAGTTTAATG TGGAAGATGG AGAGATTGTT CAGCAGGTTA GGGTTTCAAC TCCGTGGCTG 
CAGCACCTGT CTGGAGCCTG TGGTGTGGTG GATGAGGCTG TCCTGGTGTG TCCTGACCCG 
AGCTCACGTT CCCTCCAAAC TTTGGCTCTG GAGACGGAAT GGGAGTTGAG ACAGATCCCA 
CTGCAGTCTC TCGACTTAGA ATTTGGAAGT GGATTCCAAC CCCGGGTCCT GCCTACCCAG 
CCCAACCCAG TGGACGCTTC CCGGGCCCAG TTCTTCCTGC ACTTGTCCCC AAGCCACTAT 
GCTCTGCTGC AGTACCATTA TGGAACGCTG AGTTTGCTTA AAAACTTCCC ACAGACTGCC 
CTAGTGAGCT TTGCCACCAC TGGGGAGAAG ACGGTGGCTG CAGTCATGGC CTGTCGGAAT 
GAAGTGAAAA GTAGCAGTTC TGAAGATGGG TCAATGGGGA GCTTTTCGGA GAAGTCTAGT 
TCAAAGGACT CTCTGGCTTG CTTCAATCAG ACCTACACCA TTAACCTATA CCTCGTGGAG 
ACAGGTCGGC GGCTGCTGGA CACCACGATA ACATTTAGCC TGGAACAGAG CGGCACTCGG 
CCTGAGCGGC TGTATATCCA GGTGTTCTTG AAGAAGGATG ACTCAGTGGG CTACCGGGCT 
TTGGTGCAGA CAGAGGATCA TCTGCTACTT TTCCTGCAGC AGTTGGCAGG GAAGGTGGTG 
CTGTGGAGCC GTGAGGAGTC CCTGGCAGAA GTGGTGTGCC TAGAGATGGT GGACCTCCCC 
CTGACTGGGG CACAGGCCGA GCTGGAAGGA GAATTTGGCA AAAAGGCAGC AATTCAAGAT 
GGCTTGCTGG GGATGTTCCT GAAACGCCTC TCGTCTCAGC TTATCCTGCT GCAAGCATGG 
ACTTCCCACC TCTGGAAAAT GTTTTATGAT GCTCGGAAGC CCCGGAGTCA GATTAAGAAT 
GAGATCAACA TTGACACCCT GGCCAGAGAT GAATTCAACC TCCAGAAGAT GATGGTGATG 
GTAACAGCCT CAGGCAAGCT TTTTGGCATT GAGAGCAGCT CTGGCACCAT CCTGTGGAAA 
CAGTATCTAC CCAATGTCAA GCCAGACTCC TCCTTTAAAC TGATGGTCCA GAGAACTACT 
GCTCATTTCC CCCATCCCCC ACAGTGCACC CTGCTGGTGA AGGACAAGGA GTCGGGAATG 
AGTTCTCTGT ATGTCTTCAA TCCCATTTTT GGGAAGTGGA GTCAGGTAGC TCCCCCAGTG 
CTGAAGCGCC CCATCTTGCA GTCCTTGCTT CTCCCAGTCA TGGATCAAGA CTACGCCAAG 
GTGTTGCTGT TGATAGATGA TGAATACAAG GTCACAGCTT TTCCAGCCAC TCGGAATGTC 
TTGCGACAGC TACATGAGCT TGCCCCTTCC ATCTTCTTCT ATTTGGTGGA TGCAGAGCAG 
GGACGGCTGT GTGGATATCG GCTTCGAAAG GATCTCACCA CTGAGCTGAG TTGGGAGCTG 
ACCATTCCCC CAGAAGTACA GCGGATCGTC AAGGTGAAGG GGAAACGCAG CAGTGAGCAC 
GTTCATTCCC AGGGCCGTGT GATGGGGGAC CGCAGTGTGC TCTACAAGAG CCTGAACCCC 
AACCTGCTGG CCGTGGTGAC AGAGAGCACA GACGCGCACC ATGAGCGCAC CTTTATTGGC 
ATCTTCCTCA TTGATGGCGT CACTGGGCGT ATCATTCACT CCTCTGTGCA GAAGAAAGCC 
AAAGGCCCTG TCCATATCGT GCATTCAGAG AACTGGGTGG TGTACCAGTA CTGGAACACC 
AAGGCTCGGC GCAACGAGTT TACCGTACTG GAGCTCTATG AGGGCACTGA GCAATACAAC 
GCCACCGCCT TCAGCTCCCT GGACCGCCCC CAGCTGCCCC AGGTCCTCCA GCAGTCCTAT 
ATCTTCCCGT CCTCCATCAG TGCCATGGAG GCCACCATCA CCGAACGGGG CATCACCAGC 
CGACACCTGC TGATTGGACT ACCTTCTGGA GCAATTCTTT CCCTTCCTAA GGCTTTGCTG 
GATCCCCGCC GCCCCGAGAT CCCAACAGAA CAAAGCAGAG AGGAGAACTT AATCCCGTAT 
TCTCCAGATG TACAGATACA CGCAGAGCGA TTCATCAACT ATAACCAGAC AGTTTCTCGA 
ATGCGAGGTA TCTACACAGC TCCCTCGGGT CTGGAGTCCA CTTGTTTGGT TGTGGCCTAT 
GGTTTGGACA TTTACCAAAC TCGAGTCTAC CCATCCAAGC AGTTTGACGT TCTGAAGGAT 
GACTATGACT ACGTGTTAAT CAGCAGCGTC CTCTTTGGCC TGGTTTTTGC CACCATGATC 
ACTAAGAGAC TGGCACAGGT GAAGCTCCTG AATCGGGCCT GGCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001362750.1
CDS	16..3003
Translation	MAAEWASRFWLWATLLIPAAAVYEDQVGKFDWRQQYVGKVKFASLEFSPGSKKLVVATEKNVIAALNSRTGEILWRHVDKGTAEGAVDAMLLHGQDVITVSNGGRIMRSWETNIGGLNWEITLDSGSFQALGLVGLQESVRYIAVLKKTTLALHHLSSGHLKWVEHLPESDSIHYQMVYSYGSGVVWALGVVPFSHVNIVKFNVEDGEIVQQVRVSTPWLQHLSGACGVVDEAVLVCPDPSSRSLQTLALETEWELRQIPLQSLDLEFGSGFQPRVLPTQPNPVDASRAQFFLHLSPSHYALLQYHYGTLSLLKNFPQTALVSFATTGEKTVAAVMACRNEVKSSSSEDGSMGSFSEKSSSKDSLACFNQTYTINLYLVETGRRLLDTTITFSLEQSGTRPERLYIQVFLKKDDSVGYRALVQTEDHLLLFLQQLAGKVVLWSREESLAEVVCLEMVDLPLTGAQAELEGEFGKKAAIQDGLLGMFLKRLSSQLILLQAWTSHLWKMFYDARKPRSQIKNEINIDTLARDEFNLQKMMVMVTASGKLFGIESSSGTILWKQYLPNVKPDSSFKLMVQRTTAHFPHPPQCTLLVKDKESGMSSLYVFNPIFGKWSQVAPPVLKRPILQSLLLPVMDQDYAKVLLLIDDEYKVTAFPATRNVLRQLHELAPSIFFYLVDAEQGRLCGYRLRKDLTTELSWELTIPPEVQRIVKVKGKRSSEHVHSQGRVMGDRSVLYKSLNPNLLAVVTESTDAHHERTFIGIFLIDGVTGRIIHSSVQKKAKGPVHIVHSENWVVYQYWNTKARRNEFTVLELYEGTEQYNATAFSSLDRPQLPQVLQQSYIFPSSISAMEATITERGITSRHLLIGLPSGAILSLPKALLDPRRPEIPTEQSREENLIPYSPDVQIHAERFINYNQTVSRMRGIYTAPSGLESTCLVVAYGLDIYQTRVYPSKQFDVLKDDYDYVLISSVLFGLVFATMITKRLAQVKLLNRAWR

Target ORF information:

RefSeq Version	NM_001375821.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ER membrane protein complex subunit 1 (EMC1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001375821.1

ORF Insert Sequence:

ATGGCGGCTG AGTGGGCTTC TCGTTTCTGG CTTTGGGCTA CGCTGCTGAT TCCTGCGGCC 
GCGGTCTACG AAGACCAAGT GGGCAAGTTT GATTGGAGAC AGCAATATGT TGGGAAGGTC 
AAGTTTGCCT CCTTGGAATT TTCCCCTGGA TCCAAGAAGT TGGTTGTAGC CACAGAGAAG 
AATGTGATTG CAGCATTAAA TTCCCGAACT GGGGAGATCT TGTGGCGCCA TGTTGACAAG 
GGCACGGCAG AAGGGGCTGT GGATGCCATG CTGCTGCACG GACAGGATGT GATCACTGTG 
TCCAATGGAG GCCGAATCAT GCGTTCCTGG GAGACTAACA TCGGGGGCCT GAACTGGGAG 
ATAACCCTGG ACAGTGGCAG TTTCCAGGCA CTTGGGCTGG TTGGCCTGCA GGAGTCTGTA 
AGGTACATCG CAGTCCTGAA GAAGACTACA CTTGCCCTCC ATCACCTCTC CAGTGGGCAC 
CTCAAGTGGG TGGAACATCT CCCAGAAAGT GACAGCATCC ACTACCAGAT GGTGTATTCT 
TACGGCTCTG GGGTGGTGTG GGCCCTCGGA GTTGTTCCCT TCAGCCATGT GAACATTGTC 
AAGTTTAATG TGGAAGATGG AGAGATTGTT CAGCAGGTTA GGGTTTCAAC TCCGTGGCTG 
CAGCACCTGT CTGGAGCCTG TGGTGTGGTG GATGAGGCTG TCCTGGTGTG TCCTGACCCG 
AGCTCACGTT CCCTCCAAAC TTTGGCTCTG GAGACGGAAT GGGAGTTGAG ACAGATCCCA 
CTGCAGTCTC TCGACTTAGA ATTTGGAAGT GGATTCCAAC CCCGGGTCCT GCCTACCCAG 
CCCAACCCAG TGGACGCTTC CCGGGCCCAG TTCTTCCTGC ACTTGTCCCC AAGCCACTAT 
GCTCTGCTGC AGTACCATTA TGGAACGCTG AGTTTGCTTA AAAACTTCCC ACAGACTGCC 
CTAGTGAGCT TTGCCACCAC TGGGGAGAAG ACGGTGGCTG CAGTCATGGC CTGTCGGAAT 
GAAGTGAAAA GTAGCAGTTC TGAAGATGGG TCAATGGGGA GCTTTTCGGA GAAGTCTAGT 
TCAAAGGACT CTCTGGCTTG CTTCAATCAG ACCTACACCA TTAACCTATA CCTCGTGGAG 
ACAGGTCGGC GGCTGCTGGA CACCACGATA ACATTTAGCC TGGAACAGAG CGGCACTCGG 
CCTGAGCGGC TGTATATCCA GGTGTTCTTG AAGAAGGATG ACTCAGTGGG CTACCGGGCT 
TTGGTGCAGA CAGAGGATCA TCTGCTACTT TTCCTGCAGC AGTTGGCAGG GAAGGTGGTG 
CTGTGGAGCC GTGAGGAGTC CCTGGCAGAA GTGGTGTGCC TAGAGATGGT GGACCTCCCC 
CTGACTGGGG CACAGGCCGA GCTGGAAGGA GAATTTGGCA AAAAGGCAGC AATTCAAGAT 
GGCTTGCTGG GGATGTTCCT GAAACGCCTC TCGTCTCAGC TTATCCTGCT GCAAGCATGG 
ACTTCCCACC TCTGGAAAAT GTTTTATGAT GCTCGGAAGC CCCGGAGTCA GATTAAGAAT 
GAGATCAACA TTGACACCCT GGCCAGAGAT GAATTCAACC TCCAGAAGAT GATGGTGATG 
GTAACAGCCT CAGGCAAGCT TTTTGGCATT GAGAGCAGCT CTGGCACCAT CCTGTGGAAA 
CAGTATCTAC CCAATGTCAA GCCAGACTCC TCCTTTAAAC TGATGGTCCA GAGAACTACT 
GCTCATTTCC CCCATCCCCC ACAGTGCACC CTGCTGGTGA AGGACAAGGA GTCGGGAATG 
AGTTCTCTGT ATGTCTTCAA TCCCATTTTT GGGAAGTGGA GTCAGGTAGC TCCCCCAGTG 
CTGAAGCGCC CCATCTTGCA GTCCTTGCTT CTCCCAGTCA TGGATCAAGA CTACGCCAAG 
GTGTTGCTGT TGATAGATGA TGAATACAAG GTCACAGCTT TTCCAGCCAC TCGGAATGTC 
TTGCGACAGC TACATGAGCT TGCCCCTTCC ATCTTCTTCT ATTTGGTGGA TGCAGAGCAG 
GGACGGCTGT GTGGATATCG GCTTCGAAAG GATCTCACCA CTGAGCTGAG TTGGGAGCTG 
ACCATTCCCC CAGAAGTACA GCGGATCGTC AAGGTGAAGG GGAAACGCAG CAGTGAGCAC 
GTTCATTCCC AGGGCCGTGT GATGGGGGAC CGCAGTGTGC TCTACAAGAG CCTGAACCCC 
AACCTGCTGG CCGTGGTGAC AGAGAGCACA GACGCGCACC ATGAGCGCAC CTTTATTGGC 
ATCTTCCTCA TTGATGGCGT CACTGGGCGT ATCATTCACT CCTCTGTGCA GAAGAAAGCC 
AAAGGCCCTG TCCATATCGT GCATTCAGAG AACTGGGTGG TGTACCAGTA CTGGAACACC 
AAGGCTCGGC GCAACGAGTT TACCGTACTG GAGCTCTATG AGGGCACTGA GCAATACAAC 
GCCACCGCCT TCAGCTCCCT GGACCGCCCC CAGCTGCCCC AGGTCCTCCA GCAGTCCTAT 
ATCTTCCCGT CCTCCATCAG TGCCATGGAG GCCACCATCA CCGAACGGGG CATCACCAGC 
CGACACCTGC TGATTGGACT ACCTTCTGGA GCAATTCTTT CCCTTCCTAA GGCTTTGCTG 
GATCCCCGCC GCCCCGAGAT CCCAACAGAA CAAAGCAGAG AGGAGAACTT AATCCCGTAT 
TCTCCAGATG TACAGATACA CGCAGAGCGA TTCATCAACT ATAACCAGAC AGTTTCTCGA 
ATGCGAGGTA TCTACACAGC TCCCTCGGGT CTGGAGTCCA CTTGTTTGGT TGTGGCCTAT 
GGTTTGGACA TTTACCAAAC TCGAGTCTAC CCATCCAAGC AGTTTGACGT TCTGAAGGAT 
GACTATGACT ACGTGTTAAT CAGCAGCGTC CTCTTTGGCC TGGTTTTTGC CACCATGATC 
ACTAAGAGAC TGGCACAGGT GAAGCTCCTG AATCGGGCCT GGCGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	EMC1
Entrez Gene ID	23065
Full Name	ER membrane protein complex subunit 1
Synonyms	CAVIPMR,KIAA0090
General protein information	Preferred Names ER membrane protein complex subunit 1 Names ER membrane protein complex subunit 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p36.13
Summary	This gene encodes a single-pass type I transmembrane protein, which is a subunit of the endoplasmic reticulum membrane protein complex (EMC). Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2012].
Disorder MIM:	616846
Disorder Html:	Cerebellar atrophy, visual impairment, and psychomotor retardation, 616875 (3)

	EMC1	XP_001091700.1
Canis lupus familiaris (dog)	EMC1	XP_005617944.1
Drosophila melanogaster (fruit fly)	CG2943	NP_649747.1
Mus musculus (house mouse)	Emc1	NP_666269.2
Arabidopsis thaliana (thale cress)	AT5G11560	NP_196717.3
Bos taurus (cattle)	EMC1	XP_005203411.1
Danio rerio (zebrafish)	emc1	XP_693770.3
Caenorhabditis elegans (roundworm)	H17B01.4	NP_493980.1
Oryza sativa (rice)	Os05g0230600	NP_001054975.1
Gallus gallus (chicken)	EMC1	NP_001012856.1
Homo sapiens (human)	EMC1	NP_055862.1
Pan troglodytes (chimpanzee)	EMC1	XP_001162981.2
Rattus norvegicus (Norway rat)	Emc1	NP_001102160.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003647	XP_562511.3
Xenopus tropicalis (tropical clawed frog)	emc1	XP_002943252.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

EMC1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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