ALAS2 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_005261995.3	XP_005262052.1	5-aminolevulinate synthase, erythroid-specific, mitochondrial isoform X1
XM_011530771.1	XP_011529073.1	5-aminolevulinate synthase, erythroid-specific, mitochondrial isoform X3
NM_001037967.3	NP_001033056.1	5-aminolevulinate synthase, erythroid-specific, mitochondrial isoform b precursor
NM_001037968.3	NP_001033057.1	5-aminolevulinate synthase, erythroid-specific, mitochondrial isoform c precursor
XM_017029354.1	XP_016884843.1	5-aminolevulinate synthase, erythroid-specific, mitochondrial isoform X2
NM_001037967.4	NP_001033056.1	5-aminolevulinate synthase, erythroid-specific, mitochondrial isoform b precursor
NM_001037968.4	NP_001033057.1	5-aminolevulinate synthase, erythroid-specific, mitochondrial isoform c precursor
NM_000032.4	NP_000023.2	5-aminolevulinate synthase, erythroid-specific, mitochondrial isoform a precursor
NM_000032.5	NP_000023.2	5-aminolevulinate synthase, erythroid-specific, mitochondrial isoform a precursor

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18560	NM_000032.5 Latest version!	Homo sapiens 5'-aminolevulinate synthase 2 (ALAS2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu56229	XM_011530771.1 Latest version!	Homo sapiens 5'-aminolevulinate synthase 2 (ALAS2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OHu16640	NM_001037967.4 Latest version!	Homo sapiens 5'-aminolevulinate synthase 2 (ALAS2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu21664	NM_001037968.4 Latest version!	Homo sapiens 5'-aminolevulinate synthase 2 (ALAS2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu54081	XM_005261995.3 Latest version!	Homo sapiens 5'-aminolevulinate synthase 2 (ALAS2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu99203	XM_017029354.1 Latest version!	Homo sapiens 5'-aminolevulinate synthase 2 (ALAS2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu16640	NM_001037967.3	Homo sapiens 5'-aminolevulinate synthase 2 (ALAS2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu21664	NM_001037968.3	Homo sapiens 5'-aminolevulinate synthase 2 (ALAS2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu18560	NM_000032.4	Homo sapiens 5'-aminolevulinate synthase 2 (ALAS2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

ALAS2 ( NM_000032.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_000023.2 Homo sapiens 5'-aminolevulinate synthase 2 (ALAS2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18560
Clone ID Related Accession (Same CDS sequence)	NM_000032.4 , NM_000032.5
Accession Version	NM_000032.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	5-aminolevulinate synthase, erythroid-specific, mitochondrial isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK313118.1 and BQ182291.1. This sequence is a reference standard in the RefSeqGene project. On May 8, 2018 this sequence version replaced NM_000032.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X60364.1, SRR3476690.828307.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000650242.1/ ENSP00000497236.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTGACTG CAGCCATGCT GCTACAGTGC TGCCCAGTGC TTGCCCGGGG CCCCACAAGC 
CTCCTAGGCA AGGTGGTTAA GACTCACCAG TTCCTGTTTG GTATTGGACG CTGTCCCATC 
CTGGCTACCC AAGGACCAAA CTGTTCTCAA ATCCACCTTA AGGCAACAAA GGCTGGAGGA 
GATTCTCCAT CTTGGGCGAA GGGCCACTGT CCCTTCATGC TGTCGGAACT CCAGGATGGG 
AAGAGCAAGA TTGTGCAGAA GGCAGCCCCA GAAGTCCAGG AAGATGTGAA GGCTTTCAAG 
ACAGATCTGC CTAGCTCCCT GGTCTCAGTC AGCCTAAGGA AGCCATTTTC CGGTCCCCAG 
GAGCAGGAGC AGATCTCTGG GAAGGTCACA CACCTGATTC AGAACAATAT GCCTGGAAAC 
TATGTCTTCA GTTATGACCA GTTTTTCAGG GACAAGATCA TGGAGAAGAA ACAGGATCAC 
ACCTACCGTG TGTTCAAGAC TGTGAACCGC TGGGCTGATG CATATCCCTT TGCCCAACAT 
TTCTCTGAGG CATCTGTGGC CTCAAAGGAT GTGTCCGTCT GGTGTAGTAA TGATTACCTG 
GGCATGAGCC GACACCCTCA GGTCTTGCAA GCCACACAGG AGACCCTGCA GCGTCATGGT 
GCTGGAGCTG GTGGCACCCG CAACATCTCA GGCACCAGTA AGTTTCATGT GGAGCTTGAG 
CAGGAGCTGG CTGAGCTGCA CCAGAAGGAC TCAGCCCTGC TCTTCTCCTC CTGCTTTGTT 
GCCAATGACT CTACTCTCTT CACCTTGGCC AAGATCCTGC CAGGGTGCGA GATTTACTCA 
GACGCAGGCA ACCATGCTTC CATGATCCAA GGTATCCGTA ACAGTGGAGC AGCCAAGTTT 
GTCTTCAGGC ACAATGACCC TGACCACCTA AAGAAACTTC TAGAGAAGTC TAACCCTAAG 
ATACCCAAAA TTGTGGCCTT TGAGACTGTC CACTCCATGG ATGGTGCCAT CTGTCCCCTC 
GAGGAGTTGT GTGATGTGTC CCACCAGTAT GGGGCCCTGA CCTTCGTGGA TGAGGTCCAT 
GCTGTAGGAC TGTATGGGTC CCGGGGCGCT GGGATTGGGG AGCGTGATGG AATTATGCAT 
AAGATTGACA TCATCTCTGG AACTCTTGGC AAGGCCTTTG GCTGTGTGGG CGGCTACATT 
GCCAGCACCC GTGACTTGGT GGACATGGTG CGCTCCTATG CTGCAGGCTT CATCTTTACC 
ACTTCTCTGC CCCCCATGGT GCTCTCTGGA GCTCTAGAAT CTGTGCGGCT GCTCAAGGGA 
GAGGAGGGCC AAGCCCTGAG GCGAGCCCAC CAGCGCAATG TCAAGCACAT GCGCCAGCTA 
CTCATGGACA GGGGCCTTCC TGTCATCCCC TGCCCCAGCC ACATCATCCC CATCCGGGTG 
GGCAATGCAG CACTCAACAG CAAGCTCTGT GATCTCCTGC TCTCCAAGCA TGGCATCTAT 
GTGCAGGCCA TCAACTACCC AACTGTCCCC CGGGGTGAAG AGCTCCTGCG CTTGGCACCC 
TCCCCCCACC ACAGCCCTCA GATGATGGAA GATTTTGTGG AGAAGCTGCT GCTGGCTTGG 
ACTGCGGTGG GGCTGCCCCT CCAGGATGTG TCTGTGGCTG CCTGCAATTT CTGTCGCCGT 
CCTGTACACT TTGAGCTCAT GAGTGAGTGG GAACGTTCCT ACTTCGGGAA CATGGGGCCC 
CAGTATGTCA CCACCTATGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000023.2
CDS	52..1815
Translation	MVTAAMLLQCCPVLARGPTSLLGKVVKTHQFLFGIGRCPILATQGPNCSQIHLKATKAGGDSPSWAKGHCPFMLSELQDGKSKIVQKAAPEVQEDVKAFKTDLPSSLVSVSLRKPFSGPQEQEQISGKVTHLIQNNMPGNYVFSYDQFFRDKIMEKKQDHTYRVFKTVNRWADAYPFAQHFSEASVASKDVSVWCSNDYLGMSRHPQVLQATQETLQRHGAGAGGTRNISGTSKFHVELEQELAELHQKDSALLFSSCFVANDSTLFTLAKILPGCEIYSDAGNHASMIQGIRNSGAAKFVFRHNDPDHLKKLLEKSNPKIPKIVAFETVHSMDGAICPLEELCDVSHQYGALTFVDEVHAVGLYGSRGAGIGERDGIMHKIDIISGTLGKAFGCVGGYIASTRDLVDMVRSYAAGFIFTTSLPPMVLSGALESVRLLKGEEGQALRRAHQRNVKHMRQLLMDRGLPVIPCPSHIIPIRVGNAALNSKLCDLLLSKHGIYVQAINYPTVPRGEELLRLAPSPHHSPQMMEDFVEKLLLAWTAVGLPLQDVSVAACNFCRRPVHFELMSEWERSYFGNMGPQYVTTYA

Target ORF information:

RefSeq Version	NM_000032.5
Organism	Homo sapiens(human)
Definition	Homo sapiens 5'-aminolevulinate synthase 2 (ALAS2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000032.5

ORF Insert Sequence:

ATGGTGACTG CAGCCATGCT GCTACAGTGC TGCCCAGTGC TTGCCCGGGG CCCCACAAGC 
CTCCTAGGCA AGGTGGTTAA GACTCACCAG TTCCTGTTTG GTATTGGACG CTGTCCCATC 
CTGGCTACCC AAGGACCAAA CTGTTCTCAA ATCCACCTTA AGGCAACAAA GGCTGGAGGA 
GATTCTCCAT CTTGGGCGAA GGGCCACTGT CCCTTCATGC TGTCGGAACT CCAGGATGGG 
AAGAGCAAGA TTGTGCAGAA GGCAGCCCCA GAAGTCCAGG AAGATGTGAA GGCTTTCAAG 
ACAGATCTGC CTAGCTCCCT GGTCTCAGTC AGCCTAAGGA AGCCATTTTC CGGTCCCCAG 
GAGCAGGAGC AGATCTCTGG GAAGGTCACA CACCTGATTC AGAACAATAT GCCTGGAAAC 
TATGTCTTCA GTTATGACCA GTTTTTCAGG GACAAGATCA TGGAGAAGAA ACAGGATCAC 
ACCTACCGTG TGTTCAAGAC TGTGAACCGC TGGGCTGATG CATATCCCTT TGCCCAACAT 
TTCTCTGAGG CATCTGTGGC CTCAAAGGAT GTGTCCGTCT GGTGTAGTAA TGATTACCTG 
GGCATGAGCC GACACCCTCA GGTCTTGCAA GCCACACAGG AGACCCTGCA GCGTCATGGT 
GCTGGAGCTG GTGGCACCCG CAACATCTCA GGCACCAGTA AGTTTCATGT GGAGCTTGAG 
CAGGAGCTGG CTGAGCTGCA CCAGAAGGAC TCAGCCCTGC TCTTCTCCTC CTGCTTTGTT 
GCCAATGACT CTACTCTCTT CACCTTGGCC AAGATCCTGC CAGGGTGCGA GATTTACTCA 
GACGCAGGCA ACCATGCTTC CATGATCCAA GGTATCCGTA ACAGTGGAGC AGCCAAGTTT 
GTCTTCAGGC ACAATGACCC TGACCACCTA AAGAAACTTC TAGAGAAGTC TAACCCTAAG 
ATACCCAAAA TTGTGGCCTT TGAGACTGTC CACTCCATGG ATGGTGCCAT CTGTCCCCTC 
GAGGAGTTGT GTGATGTGTC CCACCAGTAT GGGGCCCTGA CCTTCGTGGA TGAGGTCCAT 
GCTGTAGGAC TGTATGGGTC CCGGGGCGCT GGGATTGGGG AGCGTGATGG AATTATGCAT 
AAGATTGACA TCATCTCTGG AACTCTTGGC AAGGCCTTTG GCTGTGTGGG CGGCTACATT 
GCCAGCACCC GTGACTTGGT GGACATGGTG CGCTCCTATG CTGCAGGCTT CATCTTTACC 
ACTTCTCTGC CCCCCATGGT GCTCTCTGGA GCTCTAGAAT CTGTGCGGCT GCTCAAGGGA 
GAGGAGGGCC AAGCCCTGAG GCGAGCCCAC CAGCGCAATG TCAAGCACAT GCGCCAGCTA 
CTCATGGACA GGGGCCTTCC TGTCATCCCC TGCCCCAGCC ACATCATCCC CATCCGGGTG 
GGCAATGCAG CACTCAACAG CAAGCTCTGT GATCTCCTGC TCTCCAAGCA TGGCATCTAT 
GTGCAGGCCA TCAACTACCC AACTGTCCCC CGGGGTGAAG AGCTCCTGCG CTTGGCACCC 
TCCCCCCACC ACAGCCCTCA GATGATGGAA GATTTTGTGG AGAAGCTGCT GCTGGCTTGG 
ACTGCGGTGG GGCTGCCCCT CCAGGATGTG TCTGTGGCTG CCTGCAATTT CTGTCGCCGT 
CCTGTACACT TTGAGCTCAT GAGTGAGTGG GAACGTTCCT ACTTCGGGAA CATGGGGCCC 
CAGTATGTCA CCACCTATGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALAS2 ( XM_011530771.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011529073.1 Homo sapiens 5'-aminolevulinate synthase 2 (ALAS2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu56229
Clone ID Related Accession (Same CDS sequence)	XM_011530771.1
Accession Version	XM_011530771.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	5-aminolevulinate synthase, erythroid-specific, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCCAAG GTATCCGTAA CAGTGGAGCA GCCAAGTTTG TCTTCAGGCA CAATGACCCT 
GACCACCTAA AGAAACTTCT AGAGAAGTCT AACCCTAAGA TACCCAAAAT TGTGGCCTTT 
GAGACTGTCC ACTCCATGGA TGGTGCCATC TGTCCCCTCG AGGAGTTGTG TGATGTGTCC 
CACCAGTATG GGGCCCTGAC CTTCGTGGAT GAGGTCCATG CTGTAGGACT GTATGGGTCC 
CGGGGCGCTG GGATTGGGGA GCGTGATGGA ATTATGCATA AGATTGACAT CATCTCTGGA 
ACTCTTGGCA AGGCCTTTGG CTGTGTGGGC GGCTACATTG CCAGCACCCG TGACTTGGTG 
GACATGGTGC GCTCCTATGC TGCAGGCTTC ATCTTTACCA CTTCTCTGCC CCCCATGGTG 
CTCTCTGGAG CTCTAGAATC TGTGCGGCTG CTCAAGGGAG AGGAGGGCCA AGCCCTGAGG 
CGAGCCCACC AGCGCAATGT CAAGCACATG CGCCAGCTAC TCATGGACAG GGGCCTTCCT 
GTCATCCCCT GCCCCAGCCA CATCATCCCC ATCCGGGTGG GCAATGCAGC ACTCAACAGC 
AAGCTCTGTG ATCTCCTGCT CTCCAAGCAT GGCATCTATG TGCAGGCCAT CAACTACCCA 
ACTGTCCCCC GGGGTGAAGA GCTCCTGCGC TTGGCACCCT CCCCCCACCA CAGCCCTCAG 
ATGATGGAAG ATTTTGTGGA GAAGCTGCTG CTGGCTTGGA CTGCGGTGGG GCTGCCCCTC 
CAGGATGTGT CTGTGGCTGC CTGCAATTTC TGTCGCCGTC CTGTACACTT TGAGCTCATG 
AGTGAGTGGG AACGTTCCTA CTTCGGGAAC ATGGGGCCCC AGTATGTCAC CACCTATGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011529073.1
CDS	579..1481
Translation	MIQGIRNSGAAKFVFRHNDPDHLKKLLEKSNPKIPKIVAFETVHSMDGAICPLEELCDVSHQYGALTFVDEVHAVGLYGSRGAGIGERDGIMHKIDIISGTLGKAFGCVGGYIASTRDLVDMVRSYAAGFIFTTSLPPMVLSGALESVRLLKGEEGQALRRAHQRNVKHMRQLLMDRGLPVIPCPSHIIPIRVGNAALNSKLCDLLLSKHGIYVQAINYPTVPRGEELLRLAPSPHHSPQMMEDFVEKLLLAWTAVGLPLQDVSVAACNFCRRPVHFELMSEWERSYFGNMGPQYVTTYA

Target ORF information:

RefSeq Version	XM_011530771.1
Organism	Homo sapiens(human)
Definition	Homo sapiens 5'-aminolevulinate synthase 2 (ALAS2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011530771.1

ORF Insert Sequence:

ATGATCCAAG GTATCCGTAA CAGTGGAGCA GCCAAGTTTG TCTTCAGGCA CAATGACCCT 
GACCACCTAA AGAAACTTCT AGAGAAGTCT AACCCTAAGA TACCCAAAAT TGTGGCCTTT 
GAGACTGTCC ACTCCATGGA TGGTGCCATC TGTCCCCTCG AGGAGTTGTG TGATGTGTCC 
CACCAGTATG GGGCCCTGAC CTTCGTGGAT GAGGTCCATG CTGTAGGACT GTATGGGTCC 
CGGGGCGCTG GGATTGGGGA GCGTGATGGA ATTATGCATA AGATTGACAT CATCTCTGGA 
ACTCTTGGCA AGGCCTTTGG CTGTGTGGGC GGCTACATTG CCAGCACCCG TGACTTGGTG 
GACATGGTGC GCTCCTATGC TGCAGGCTTC ATCTTTACCA CTTCTCTGCC CCCCATGGTG 
CTCTCTGGAG CTCTAGAATC TGTGCGGCTG CTCAAGGGAG AGGAGGGCCA AGCCCTGAGG 
CGAGCCCACC AGCGCAATGT CAAGCACATG CGCCAGCTAC TCATGGACAG GGGCCTTCCT 
GTCATCCCCT GCCCCAGCCA CATCATCCCC ATCCGGGTGG GCAATGCAGC ACTCAACAGC 
AAGCTCTGTG ATCTCCTGCT CTCCAAGCAT GGCATCTATG TGCAGGCCAT CAACTACCCA 
ACTGTCCCCC GGGGTGAAGA GCTCCTGCGC TTGGCACCCT CCCCCCACCA CAGCCCTCAG 
ATGATGGAAG ATTTTGTGGA GAAGCTGCTG CTGGCTTGGA CTGCGGTGGG GCTGCCCCTC 
CAGGATGTGT CTGTGGCTGC CTGCAATTTC TGTCGCCGTC CTGTACACTT TGAGCTCATG 
AGTGAGTGGG AACGTTCCTA CTTCGGGAAC ATGGGGCCCC AGTATGTCAC CACCTATGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALAS2 ( NM_001037967.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001033056.1 Homo sapiens 5'-aminolevulinate synthase 2 (ALAS2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu16640
Clone ID Related Accession (Same CDS sequence)	NM_001037967.3 , NM_001037967.4
Accession Version	NM_001037967.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-27
Organism	Homo sapiens(human)
Product	5-aminolevulinate synthase, erythroid-specific, mitochondrial isoform b precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP284609.1, AK291589.1 and BQ182291.1. On Aug 2, 2008 this sequence version replaced NM_001037967.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK291589.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149004 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTGACTG CAGCCATGCT GCTACAGTGC TGCCCAGTGC TTGCCCGGGG CCCCACAAGC 
CTCCTAGGCA AGGTGGTTAA GACTCACCAG TTCCTGTTTG GTATTGGACG CTGTCCCATC 
CTGGCTACCC AAGGACCAAA CTGTTCTCAA ATCCACCTTA AGGCAACAAA GGCTGGAGGA 
GATTCTCCAT CTTGGGCGAA GGGCCACTGT CCCTTCATGC TGTCGGAACT CCAGGATGGG 
AAGAGCAAGA TTGTGCAGAA GGCAGCCCCA GAAGTCCAGG AAGATGTGAA GGCTTTCAAG 
ACAGGAAACT ATGTCTTCAG TTATGACCAG TTTTTCAGGG ACAAGATCAT GGAGAAGAAA 
CAGGATCACA CCTACCGTGT GTTCAAGACT GTGAACCGCT GGGCTGATGC ATATCCCTTT 
GCCCAACATT TCTCTGAGGC ATCTGTGGCC TCAAAGGATG TGTCCGTCTG GTGTAGTAAT 
GATTACCTGG GCATGAGCCG ACACCCTCAG GTCTTGCAAG CCACACAGGA GACCCTGCAG 
CGTCATGGTG CTGGAGCTGG TGGCACCCGC AACATCTCAG GCACCAGTAA GTTTCATGTG 
GAGCTTGAGC AGGAGCTGGC TGAGCTGCAC CAGAAGGACT CAGCCCTGCT CTTCTCCTCC 
TGCTTTGTTG CCAATGACTC TACTCTCTTC ACCTTGGCCA AGATCCTGCC AGGGTGCGAG 
ATTTACTCAG ACGCAGGCAA CCATGCTTCC ATGATCCAAG GTATCCGTAA CAGTGGAGCA 
GCCAAGTTTG TCTTCAGGCA CAATGACCCT GACCACCTAA AGAAACTTCT AGAGAAGTCT 
AACCCTAAGA TACCCAAAAT TGTGGCCTTT GAGACTGTCC ACTCCATGGA TGGTGCCATC 
TGTCCCCTCG AGGAGTTGTG TGATGTGTCC CACCAGTATG GGGCCCTGAC CTTCGTGGAT 
GAGGTCCATG CTGTAGGACT GTATGGGTCC CGGGGCGCTG GGATTGGGGA GCGTGATGGA 
ATTATGCATA AGATTGACAT CATCTCTGGA ACTCTTGGCA AGGCCTTTGG CTGTGTGGGC 
GGCTACATTG CCAGCACCCG TGACTTGGTG GACATGGTGC GCTCCTATGC TGCAGGCTTC 
ATCTTTACCA CTTCTCTGCC CCCCATGGTG CTCTCTGGAG CTCTAGAATC TGTGCGGCTG 
CTCAAGGGAG AGGAGGGCCA AGCCCTGAGG CGAGCCCACC AGCGCAATGT CAAGCACATG 
CGCCAGCTAC TCATGGACAG GGGCCTTCCT GTCATCCCCT GCCCCAGCCA CATCATCCCC 
ATCCGGGTGG GCAATGCAGC ACTCAACAGC AAGCTCTGTG ATCTCCTGCT CTCCAAGCAT 
GGCATCTATG TGCAGGCCAT CAACTACCCA ACTGTCCCCC GGGGTGAAGA GCTCCTGCGC 
TTGGCACCCT CCCCCCACCA CAGCCCTCAG ATGATGGAAG ATTTTGTGGA GAAGCTGCTG 
CTGGCTTGGA CTGCGGTGGG GCTGCCCCTC CAGGATGTGT CTGTGGCTGC CTGCAATTTC 
TGTCGCCGTC CTGTACACTT TGAGCTCATG AGTGAGTGGG AACGTTCCTA CTTCGGGAAC 
ATGGGGCCCC AGTATGTCAC CACCTATGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001033056.1
CDS	139..1791
Translation	MVTAAMLLQCCPVLARGPTSLLGKVVKTHQFLFGIGRCPILATQGPNCSQIHLKATKAGGDSPSWAKGHCPFMLSELQDGKSKIVQKAAPEVQEDVKAFKTGNYVFSYDQFFRDKIMEKKQDHTYRVFKTVNRWADAYPFAQHFSEASVASKDVSVWCSNDYLGMSRHPQVLQATQETLQRHGAGAGGTRNISGTSKFHVELEQELAELHQKDSALLFSSCFVANDSTLFTLAKILPGCEIYSDAGNHASMIQGIRNSGAAKFVFRHNDPDHLKKLLEKSNPKIPKIVAFETVHSMDGAICPLEELCDVSHQYGALTFVDEVHAVGLYGSRGAGIGERDGIMHKIDIISGTLGKAFGCVGGYIASTRDLVDMVRSYAAGFIFTTSLPPMVLSGALESVRLLKGEEGQALRRAHQRNVKHMRQLLMDRGLPVIPCPSHIIPIRVGNAALNSKLCDLLLSKHGIYVQAINYPTVPRGEELLRLAPSPHHSPQMMEDFVEKLLLAWTAVGLPLQDVSVAACNFCRRPVHFELMSEWERSYFGNMGPQYVTTYA

Target ORF information:

RefSeq Version	NM_001037967.3
Organism	Homo sapiens(human)
Definition	Homo sapiens 5'-aminolevulinate synthase 2 (ALAS2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001037967.3

ORF Insert Sequence:

ATGGTGACTG CAGCCATGCT GCTACAGTGC TGCCCAGTGC TTGCCCGGGG CCCCACAAGC 
CTCCTAGGCA AGGTGGTTAA GACTCACCAG TTCCTGTTTG GTATTGGACG CTGTCCCATC 
CTGGCTACCC AAGGACCAAA CTGTTCTCAA ATCCACCTTA AGGCAACAAA GGCTGGAGGA 
GATTCTCCAT CTTGGGCGAA GGGCCACTGT CCCTTCATGC TGTCGGAACT CCAGGATGGG 
AAGAGCAAGA TTGTGCAGAA GGCAGCCCCA GAAGTCCAGG AAGATGTGAA GGCTTTCAAG 
ACAGGAAACT ATGTCTTCAG TTATGACCAG TTTTTCAGGG ACAAGATCAT GGAGAAGAAA 
CAGGATCACA CCTACCGTGT GTTCAAGACT GTGAACCGCT GGGCTGATGC ATATCCCTTT 
GCCCAACATT TCTCTGAGGC ATCTGTGGCC TCAAAGGATG TGTCCGTCTG GTGTAGTAAT 
GATTACCTGG GCATGAGCCG ACACCCTCAG GTCTTGCAAG CCACACAGGA GACCCTGCAG 
CGTCATGGTG CTGGAGCTGG TGGCACCCGC AACATCTCAG GCACCAGTAA GTTTCATGTG 
GAGCTTGAGC AGGAGCTGGC TGAGCTGCAC CAGAAGGACT CAGCCCTGCT CTTCTCCTCC 
TGCTTTGTTG CCAATGACTC TACTCTCTTC ACCTTGGCCA AGATCCTGCC AGGGTGCGAG 
ATTTACTCAG ACGCAGGCAA CCATGCTTCC ATGATCCAAG GTATCCGTAA CAGTGGAGCA 
GCCAAGTTTG TCTTCAGGCA CAATGACCCT GACCACCTAA AGAAACTTCT AGAGAAGTCT 
AACCCTAAGA TACCCAAAAT TGTGGCCTTT GAGACTGTCC ACTCCATGGA TGGTGCCATC 
TGTCCCCTCG AGGAGTTGTG TGATGTGTCC CACCAGTATG GGGCCCTGAC CTTCGTGGAT 
GAGGTCCATG CTGTAGGACT GTATGGGTCC CGGGGCGCTG GGATTGGGGA GCGTGATGGA 
ATTATGCATA AGATTGACAT CATCTCTGGA ACTCTTGGCA AGGCCTTTGG CTGTGTGGGC 
GGCTACATTG CCAGCACCCG TGACTTGGTG GACATGGTGC GCTCCTATGC TGCAGGCTTC 
ATCTTTACCA CTTCTCTGCC CCCCATGGTG CTCTCTGGAG CTCTAGAATC TGTGCGGCTG 
CTCAAGGGAG AGGAGGGCCA AGCCCTGAGG CGAGCCCACC AGCGCAATGT CAAGCACATG 
CGCCAGCTAC TCATGGACAG GGGCCTTCCT GTCATCCCCT GCCCCAGCCA CATCATCCCC 
ATCCGGGTGG GCAATGCAGC ACTCAACAGC AAGCTCTGTG ATCTCCTGCT CTCCAAGCAT 
GGCATCTATG TGCAGGCCAT CAACTACCCA ACTGTCCCCC GGGGTGAAGA GCTCCTGCGC 
TTGGCACCCT CCCCCCACCA CAGCCCTCAG ATGATGGAAG ATTTTGTGGA GAAGCTGCTG 
CTGGCTTGGA CTGCGGTGGG GCTGCCCCTC CAGGATGTGT CTGTGGCTGC CTGCAATTTC 
TGTCGCCGTC CTGTACACTT TGAGCTCATG AGTGAGTGGG AACGTTCCTA CTTCGGGAAC 
ATGGGGCCCC AGTATGTCAC CACCTATGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALAS2 ( NM_001037967.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001033056.1 Homo sapiens 5'-aminolevulinate synthase 2 (ALAS2), transcript variant 2, mRNA.

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CloneID	OHu16640
Clone ID Related Accession (Same CDS sequence)	NM_001037967.3 , NM_001037967.4
Accession Version	NM_001037967.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	5-aminolevulinate synthase, erythroid-specific, mitochondrial isoform b precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK291589.1 and BQ182291.1. On May 31, 2019 this sequence version replaced NM_001037967.3. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK291589.1, SRR7410570.689895.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149004 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTGACTG CAGCCATGCT GCTACAGTGC TGCCCAGTGC TTGCCCGGGG CCCCACAAGC 
CTCCTAGGCA AGGTGGTTAA GACTCACCAG TTCCTGTTTG GTATTGGACG CTGTCCCATC 
CTGGCTACCC AAGGACCAAA CTGTTCTCAA ATCCACCTTA AGGCAACAAA GGCTGGAGGA 
GATTCTCCAT CTTGGGCGAA GGGCCACTGT CCCTTCATGC TGTCGGAACT CCAGGATGGG 
AAGAGCAAGA TTGTGCAGAA GGCAGCCCCA GAAGTCCAGG AAGATGTGAA GGCTTTCAAG 
ACAGGAAACT ATGTCTTCAG TTATGACCAG TTTTTCAGGG ACAAGATCAT GGAGAAGAAA 
CAGGATCACA CCTACCGTGT GTTCAAGACT GTGAACCGCT GGGCTGATGC ATATCCCTTT 
GCCCAACATT TCTCTGAGGC ATCTGTGGCC TCAAAGGATG TGTCCGTCTG GTGTAGTAAT 
GATTACCTGG GCATGAGCCG ACACCCTCAG GTCTTGCAAG CCACACAGGA GACCCTGCAG 
CGTCATGGTG CTGGAGCTGG TGGCACCCGC AACATCTCAG GCACCAGTAA GTTTCATGTG 
GAGCTTGAGC AGGAGCTGGC TGAGCTGCAC CAGAAGGACT CAGCCCTGCT CTTCTCCTCC 
TGCTTTGTTG CCAATGACTC TACTCTCTTC ACCTTGGCCA AGATCCTGCC AGGGTGCGAG 
ATTTACTCAG ACGCAGGCAA CCATGCTTCC ATGATCCAAG GTATCCGTAA CAGTGGAGCA 
GCCAAGTTTG TCTTCAGGCA CAATGACCCT GACCACCTAA AGAAACTTCT AGAGAAGTCT 
AACCCTAAGA TACCCAAAAT TGTGGCCTTT GAGACTGTCC ACTCCATGGA TGGTGCCATC 
TGTCCCCTCG AGGAGTTGTG TGATGTGTCC CACCAGTATG GGGCCCTGAC CTTCGTGGAT 
GAGGTCCATG CTGTAGGACT GTATGGGTCC CGGGGCGCTG GGATTGGGGA GCGTGATGGA 
ATTATGCATA AGATTGACAT CATCTCTGGA ACTCTTGGCA AGGCCTTTGG CTGTGTGGGC 
GGCTACATTG CCAGCACCCG TGACTTGGTG GACATGGTGC GCTCCTATGC TGCAGGCTTC 
ATCTTTACCA CTTCTCTGCC CCCCATGGTG CTCTCTGGAG CTCTAGAATC TGTGCGGCTG 
CTCAAGGGAG AGGAGGGCCA AGCCCTGAGG CGAGCCCACC AGCGCAATGT CAAGCACATG 
CGCCAGCTAC TCATGGACAG GGGCCTTCCT GTCATCCCCT GCCCCAGCCA CATCATCCCC 
ATCCGGGTGG GCAATGCAGC ACTCAACAGC AAGCTCTGTG ATCTCCTGCT CTCCAAGCAT 
GGCATCTATG TGCAGGCCAT CAACTACCCA ACTGTCCCCC GGGGTGAAGA GCTCCTGCGC 
TTGGCACCCT CCCCCCACCA CAGCCCTCAG ATGATGGAAG ATTTTGTGGA GAAGCTGCTG 
CTGGCTTGGA CTGCGGTGGG GCTGCCCCTC CAGGATGTGT CTGTGGCTGC CTGCAATTTC 
TGTCGCCGTC CTGTACACTT TGAGCTCATG AGTGAGTGGG AACGTTCCTA CTTCGGGAAC 
ATGGGGCCCC AGTATGTCAC CACCTATGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001033056.1
CDS	52..1704
Translation	MVTAAMLLQCCPVLARGPTSLLGKVVKTHQFLFGIGRCPILATQGPNCSQIHLKATKAGGDSPSWAKGHCPFMLSELQDGKSKIVQKAAPEVQEDVKAFKTGNYVFSYDQFFRDKIMEKKQDHTYRVFKTVNRWADAYPFAQHFSEASVASKDVSVWCSNDYLGMSRHPQVLQATQETLQRHGAGAGGTRNISGTSKFHVELEQELAELHQKDSALLFSSCFVANDSTLFTLAKILPGCEIYSDAGNHASMIQGIRNSGAAKFVFRHNDPDHLKKLLEKSNPKIPKIVAFETVHSMDGAICPLEELCDVSHQYGALTFVDEVHAVGLYGSRGAGIGERDGIMHKIDIISGTLGKAFGCVGGYIASTRDLVDMVRSYAAGFIFTTSLPPMVLSGALESVRLLKGEEGQALRRAHQRNVKHMRQLLMDRGLPVIPCPSHIIPIRVGNAALNSKLCDLLLSKHGIYVQAINYPTVPRGEELLRLAPSPHHSPQMMEDFVEKLLLAWTAVGLPLQDVSVAACNFCRRPVHFELMSEWERSYFGNMGPQYVTTYA

Target ORF information:

RefSeq Version	NM_001037967.4
Organism	Homo sapiens(human)
Definition	Homo sapiens 5'-aminolevulinate synthase 2 (ALAS2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001037967.4

ORF Insert Sequence:

ATGGTGACTG CAGCCATGCT GCTACAGTGC TGCCCAGTGC TTGCCCGGGG CCCCACAAGC 
CTCCTAGGCA AGGTGGTTAA GACTCACCAG TTCCTGTTTG GTATTGGACG CTGTCCCATC 
CTGGCTACCC AAGGACCAAA CTGTTCTCAA ATCCACCTTA AGGCAACAAA GGCTGGAGGA 
GATTCTCCAT CTTGGGCGAA GGGCCACTGT CCCTTCATGC TGTCGGAACT CCAGGATGGG 
AAGAGCAAGA TTGTGCAGAA GGCAGCCCCA GAAGTCCAGG AAGATGTGAA GGCTTTCAAG 
ACAGGAAACT ATGTCTTCAG TTATGACCAG TTTTTCAGGG ACAAGATCAT GGAGAAGAAA 
CAGGATCACA CCTACCGTGT GTTCAAGACT GTGAACCGCT GGGCTGATGC ATATCCCTTT 
GCCCAACATT TCTCTGAGGC ATCTGTGGCC TCAAAGGATG TGTCCGTCTG GTGTAGTAAT 
GATTACCTGG GCATGAGCCG ACACCCTCAG GTCTTGCAAG CCACACAGGA GACCCTGCAG 
CGTCATGGTG CTGGAGCTGG TGGCACCCGC AACATCTCAG GCACCAGTAA GTTTCATGTG 
GAGCTTGAGC AGGAGCTGGC TGAGCTGCAC CAGAAGGACT CAGCCCTGCT CTTCTCCTCC 
TGCTTTGTTG CCAATGACTC TACTCTCTTC ACCTTGGCCA AGATCCTGCC AGGGTGCGAG 
ATTTACTCAG ACGCAGGCAA CCATGCTTCC ATGATCCAAG GTATCCGTAA CAGTGGAGCA 
GCCAAGTTTG TCTTCAGGCA CAATGACCCT GACCACCTAA AGAAACTTCT AGAGAAGTCT 
AACCCTAAGA TACCCAAAAT TGTGGCCTTT GAGACTGTCC ACTCCATGGA TGGTGCCATC 
TGTCCCCTCG AGGAGTTGTG TGATGTGTCC CACCAGTATG GGGCCCTGAC CTTCGTGGAT 
GAGGTCCATG CTGTAGGACT GTATGGGTCC CGGGGCGCTG GGATTGGGGA GCGTGATGGA 
ATTATGCATA AGATTGACAT CATCTCTGGA ACTCTTGGCA AGGCCTTTGG CTGTGTGGGC 
GGCTACATTG CCAGCACCCG TGACTTGGTG GACATGGTGC GCTCCTATGC TGCAGGCTTC 
ATCTTTACCA CTTCTCTGCC CCCCATGGTG CTCTCTGGAG CTCTAGAATC TGTGCGGCTG 
CTCAAGGGAG AGGAGGGCCA AGCCCTGAGG CGAGCCCACC AGCGCAATGT CAAGCACATG 
CGCCAGCTAC TCATGGACAG GGGCCTTCCT GTCATCCCCT GCCCCAGCCA CATCATCCCC 
ATCCGGGTGG GCAATGCAGC ACTCAACAGC AAGCTCTGTG ATCTCCTGCT CTCCAAGCAT 
GGCATCTATG TGCAGGCCAT CAACTACCCA ACTGTCCCCC GGGGTGAAGA GCTCCTGCGC 
TTGGCACCCT CCCCCCACCA CAGCCCTCAG ATGATGGAAG ATTTTGTGGA GAAGCTGCTG 
CTGGCTTGGA CTGCGGTGGG GCTGCCCCTC CAGGATGTGT CTGTGGCTGC CTGCAATTTC 
TGTCGCCGTC CTGTACACTT TGAGCTCATG AGTGAGTGGG AACGTTCCTA CTTCGGGAAC 
ATGGGGCCCC AGTATGTCAC CACCTATGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALAS2 ( NM_000032.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000023.2 Homo sapiens 5'-aminolevulinate synthase 2 (ALAS2), transcript variant 1, mRNA.

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CloneID	OHu18560
Clone ID Related Accession (Same CDS sequence)	NM_000032.4 , NM_000032.5
Accession Version	NM_000032.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	5-aminolevulinate synthase, erythroid-specific, mitochondrial isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK313118.1 and BQ182291.1. This sequence is a reference standard in the RefSeqGene project. On Aug 2, 2008 this sequence version replaced NM_000032.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X60364.1, AK313118.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTGACTG CAGCCATGCT GCTACAGTGC TGCCCAGTGC TTGCCCGGGG CCCCACAAGC 
CTCCTAGGCA AGGTGGTTAA GACTCACCAG TTCCTGTTTG GTATTGGACG CTGTCCCATC 
CTGGCTACCC AAGGACCAAA CTGTTCTCAA ATCCACCTTA AGGCAACAAA GGCTGGAGGA 
GATTCTCCAT CTTGGGCGAA GGGCCACTGT CCCTTCATGC TGTCGGAACT CCAGGATGGG 
AAGAGCAAGA TTGTGCAGAA GGCAGCCCCA GAAGTCCAGG AAGATGTGAA GGCTTTCAAG 
ACAGATCTGC CTAGCTCCCT GGTCTCAGTC AGCCTAAGGA AGCCATTTTC CGGTCCCCAG 
GAGCAGGAGC AGATCTCTGG GAAGGTCACA CACCTGATTC AGAACAATAT GCCTGGAAAC 
TATGTCTTCA GTTATGACCA GTTTTTCAGG GACAAGATCA TGGAGAAGAA ACAGGATCAC 
ACCTACCGTG TGTTCAAGAC TGTGAACCGC TGGGCTGATG CATATCCCTT TGCCCAACAT 
TTCTCTGAGG CATCTGTGGC CTCAAAGGAT GTGTCCGTCT GGTGTAGTAA TGATTACCTG 
GGCATGAGCC GACACCCTCA GGTCTTGCAA GCCACACAGG AGACCCTGCA GCGTCATGGT 
GCTGGAGCTG GTGGCACCCG CAACATCTCA GGCACCAGTA AGTTTCATGT GGAGCTTGAG 
CAGGAGCTGG CTGAGCTGCA CCAGAAGGAC TCAGCCCTGC TCTTCTCCTC CTGCTTTGTT 
GCCAATGACT CTACTCTCTT CACCTTGGCC AAGATCCTGC CAGGGTGCGA GATTTACTCA 
GACGCAGGCA ACCATGCTTC CATGATCCAA GGTATCCGTA ACAGTGGAGC AGCCAAGTTT 
GTCTTCAGGC ACAATGACCC TGACCACCTA AAGAAACTTC TAGAGAAGTC TAACCCTAAG 
ATACCCAAAA TTGTGGCCTT TGAGACTGTC CACTCCATGG ATGGTGCCAT CTGTCCCCTC 
GAGGAGTTGT GTGATGTGTC CCACCAGTAT GGGGCCCTGA CCTTCGTGGA TGAGGTCCAT 
GCTGTAGGAC TGTATGGGTC CCGGGGCGCT GGGATTGGGG AGCGTGATGG AATTATGCAT 
AAGATTGACA TCATCTCTGG AACTCTTGGC AAGGCCTTTG GCTGTGTGGG CGGCTACATT 
GCCAGCACCC GTGACTTGGT GGACATGGTG CGCTCCTATG CTGCAGGCTT CATCTTTACC 
ACTTCTCTGC CCCCCATGGT GCTCTCTGGA GCTCTAGAAT CTGTGCGGCT GCTCAAGGGA 
GAGGAGGGCC AAGCCCTGAG GCGAGCCCAC CAGCGCAATG TCAAGCACAT GCGCCAGCTA 
CTCATGGACA GGGGCCTTCC TGTCATCCCC TGCCCCAGCC ACATCATCCC CATCCGGGTG 
GGCAATGCAG CACTCAACAG CAAGCTCTGT GATCTCCTGC TCTCCAAGCA TGGCATCTAT 
GTGCAGGCCA TCAACTACCC AACTGTCCCC CGGGGTGAAG AGCTCCTGCG CTTGGCACCC 
TCCCCCCACC ACAGCCCTCA GATGATGGAA GATTTTGTGG AGAAGCTGCT GCTGGCTTGG 
ACTGCGGTGG GGCTGCCCCT CCAGGATGTG TCTGTGGCTG CCTGCAATTT CTGTCGCCGT 
CCTGTACACT TTGAGCTCAT GAGTGAGTGG GAACGTTCCT ACTTCGGGAA CATGGGGCCC 
CAGTATGTCA CCACCTATGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000023.2
CDS	139..1902
Translation	MVTAAMLLQCCPVLARGPTSLLGKVVKTHQFLFGIGRCPILATQGPNCSQIHLKATKAGGDSPSWAKGHCPFMLSELQDGKSKIVQKAAPEVQEDVKAFKTDLPSSLVSVSLRKPFSGPQEQEQISGKVTHLIQNNMPGNYVFSYDQFFRDKIMEKKQDHTYRVFKTVNRWADAYPFAQHFSEASVASKDVSVWCSNDYLGMSRHPQVLQATQETLQRHGAGAGGTRNISGTSKFHVELEQELAELHQKDSALLFSSCFVANDSTLFTLAKILPGCEIYSDAGNHASMIQGIRNSGAAKFVFRHNDPDHLKKLLEKSNPKIPKIVAFETVHSMDGAICPLEELCDVSHQYGALTFVDEVHAVGLYGSRGAGIGERDGIMHKIDIISGTLGKAFGCVGGYIASTRDLVDMVRSYAAGFIFTTSLPPMVLSGALESVRLLKGEEGQALRRAHQRNVKHMRQLLMDRGLPVIPCPSHIIPIRVGNAALNSKLCDLLLSKHGIYVQAINYPTVPRGEELLRLAPSPHHSPQMMEDFVEKLLLAWTAVGLPLQDVSVAACNFCRRPVHFELMSEWERSYFGNMGPQYVTTYA

Target ORF information:

RefSeq Version	NM_000032.4
Organism	Homo sapiens(human)
Definition	Homo sapiens 5'-aminolevulinate synthase 2 (ALAS2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000032.4

ORF Insert Sequence:

ATGGTGACTG CAGCCATGCT GCTACAGTGC TGCCCAGTGC TTGCCCGGGG CCCCACAAGC 
CTCCTAGGCA AGGTGGTTAA GACTCACCAG TTCCTGTTTG GTATTGGACG CTGTCCCATC 
CTGGCTACCC AAGGACCAAA CTGTTCTCAA ATCCACCTTA AGGCAACAAA GGCTGGAGGA 
GATTCTCCAT CTTGGGCGAA GGGCCACTGT CCCTTCATGC TGTCGGAACT CCAGGATGGG 
AAGAGCAAGA TTGTGCAGAA GGCAGCCCCA GAAGTCCAGG AAGATGTGAA GGCTTTCAAG 
ACAGATCTGC CTAGCTCCCT GGTCTCAGTC AGCCTAAGGA AGCCATTTTC CGGTCCCCAG 
GAGCAGGAGC AGATCTCTGG GAAGGTCACA CACCTGATTC AGAACAATAT GCCTGGAAAC 
TATGTCTTCA GTTATGACCA GTTTTTCAGG GACAAGATCA TGGAGAAGAA ACAGGATCAC 
ACCTACCGTG TGTTCAAGAC TGTGAACCGC TGGGCTGATG CATATCCCTT TGCCCAACAT 
TTCTCTGAGG CATCTGTGGC CTCAAAGGAT GTGTCCGTCT GGTGTAGTAA TGATTACCTG 
GGCATGAGCC GACACCCTCA GGTCTTGCAA GCCACACAGG AGACCCTGCA GCGTCATGGT 
GCTGGAGCTG GTGGCACCCG CAACATCTCA GGCACCAGTA AGTTTCATGT GGAGCTTGAG 
CAGGAGCTGG CTGAGCTGCA CCAGAAGGAC TCAGCCCTGC TCTTCTCCTC CTGCTTTGTT 
GCCAATGACT CTACTCTCTT CACCTTGGCC AAGATCCTGC CAGGGTGCGA GATTTACTCA 
GACGCAGGCA ACCATGCTTC CATGATCCAA GGTATCCGTA ACAGTGGAGC AGCCAAGTTT 
GTCTTCAGGC ACAATGACCC TGACCACCTA AAGAAACTTC TAGAGAAGTC TAACCCTAAG 
ATACCCAAAA TTGTGGCCTT TGAGACTGTC CACTCCATGG ATGGTGCCAT CTGTCCCCTC 
GAGGAGTTGT GTGATGTGTC CCACCAGTAT GGGGCCCTGA CCTTCGTGGA TGAGGTCCAT 
GCTGTAGGAC TGTATGGGTC CCGGGGCGCT GGGATTGGGG AGCGTGATGG AATTATGCAT 
AAGATTGACA TCATCTCTGG AACTCTTGGC AAGGCCTTTG GCTGTGTGGG CGGCTACATT 
GCCAGCACCC GTGACTTGGT GGACATGGTG CGCTCCTATG CTGCAGGCTT CATCTTTACC 
ACTTCTCTGC CCCCCATGGT GCTCTCTGGA GCTCTAGAAT CTGTGCGGCT GCTCAAGGGA 
GAGGAGGGCC AAGCCCTGAG GCGAGCCCAC CAGCGCAATG TCAAGCACAT GCGCCAGCTA 
CTCATGGACA GGGGCCTTCC TGTCATCCCC TGCCCCAGCC ACATCATCCC CATCCGGGTG 
GGCAATGCAG CACTCAACAG CAAGCTCTGT GATCTCCTGC TCTCCAAGCA TGGCATCTAT 
GTGCAGGCCA TCAACTACCC AACTGTCCCC CGGGGTGAAG AGCTCCTGCG CTTGGCACCC 
TCCCCCCACC ACAGCCCTCA GATGATGGAA GATTTTGTGG AGAAGCTGCT GCTGGCTTGG 
ACTGCGGTGG GGCTGCCCCT CCAGGATGTG TCTGTGGCTG CCTGCAATTT CTGTCGCCGT 
CCTGTACACT TTGAGCTCAT GAGTGAGTGG GAACGTTCCT ACTTCGGGAA CATGGGGCCC 
CAGTATGTCA CCACCTATGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALAS2 ( NM_001037968.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001033057.1 Homo sapiens 5'-aminolevulinate synthase 2 (ALAS2), transcript variant 3, mRNA.

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CloneID	OHu21664
Clone ID Related Accession (Same CDS sequence)	NM_001037968.3 , NM_001037968.4
Accession Version	NM_001037968.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-27
Organism	Homo sapiens(human)
Product	5-aminolevulinate synthase, erythroid-specific, mitochondrial isoform c precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP284609.1, BP233279.1, AL020991.1 and BQ182291.1. On Aug 2, 2008 this sequence version replaced NM_001037968.2. Transcript Variant: This variant (3) differs in the 5' UTR, has an additional 5' exon resulting in an alternate start codon, and lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. The resulting isoform (c) is shorter but has a longer and distinct N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC030230.2 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2149004 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGAGGGG GAGAGGTTGC TCTGGGATGG AACAAGAAAA AGAGGTTGTT TTGTGAGGAC 
TTTAGGTTCA AGATGGTGAC TGCAGCCATG CTGCTACAGT GCTGCCCAGT GCTTGCCCGG 
GGCCCCACAA GCCTCCTAGG CAAGGTGGTT AAGACTCACC AGTTCCTGTT TGGTATTGGA 
CGCTGTCCCA TCCTGGCTAC CCAAGGACCA AACTGTTCTC AAATCCACCT TAAGGCAACA 
AAGGCTGGAG GAGATTCTCC ATCTTGGGCG AAGGGCCACT GTCCCTTCAT GCTGTCGGAA 
CTCCAGGATG GGAAGAGCAA GATTGTGCAG AAGGCAGCCC CAGAAGTCCA GGAAGATGTG 
AAGGCTTTCA AGACAGGAAA CTATGTCTTC AGTTATGACC AGTTTTTCAG GGACAAGATC 
ATGGAGAAGA AACAGGATCA CACCTACCGT GTGTTCAAGA CTGTGAACCG CTGGGCTGAT 
GCATATCCCT TTGCCCAACA TTTCTCTGAG GCATCTGTGG CCTCAAAGGA TGTGTCCGTC 
TGGTGTAGTA ATGATTACCT GGGCATGAGC CGACACCCTC AGGTCTTGCA AGCCACACAG 
GAGACCCTGC AGCGTCATGG TGCTGGAGCT GGTGGCACCC GCAACATCTC AGGCACCAGT 
AAGTTTCATG TGGAGCTTGA GCAGGAGCTG GCTGAGCTGC ACCAGAAGGA CTCAGCCCTG 
CTCTTCTCCT CCTGCTTTGT TGCCAATGAC TCTACTCTCT TCACCTTGGC CAAGATCCTG 
CCAGGGTGCG AGATTTACTC AGACGCAGGC AACCATGCTT CCATGATCCA AGGTATCCGT 
AACAGTGGAG CAGCCAAGTT TGTCTTCAGG CACAATGACC CTGACCACCT AAAGAAACTT 
CTAGAGAAGT CTAACCCTAA GATACCCAAA ATTGTGGCCT TTGAGACTGT CCACTCCATG 
GATGGTGCCA TCTGTCCCCT CGAGGAGTTG TGTGATGTGT CCCACCAGTA TGGGGCCCTG 
ACCTTCGTGG ATGAGGTCCA TGCTGTAGGA CTGTATGGGT CCCGGGGCGC TGGGATTGGG 
GAGCGTGATG GAATTATGCA TAAGATTGAC ATCATCTCTG GAACTCTTGG CAAGGCCTTT 
GGCTGTGTGG GCGGCTACAT TGCCAGCACC CGTGACTTGG TGGACATGGT GCGCTCCTAT 
GCTGCAGGCT TCATCTTTAC CACTTCTCTG CCCCCCATGG TGCTCTCTGG AGCTCTAGAA 
TCTGTGCGGC TGCTCAAGGG AGAGGAGGGC CAAGCCCTGA GGCGAGCCCA CCAGCGCAAT 
GTCAAGCACA TGCGCCAGCT ACTCATGGAC AGGGGCCTTC CTGTCATCCC CTGCCCCAGC 
CACATCATCC CCATCCGGGT GGGCAATGCA GCACTCAACA GCAAGCTCTG TGATCTCCTG 
CTCTCCAAGC ATGGCATCTA TGTGCAGGCC ATCAACTACC CAACTGTCCC CCGGGGTGAA 
GAGCTCCTGC GCTTGGCACC CTCCCCCCAC CACAGCCCTC AGATGATGGA AGATTTTGTG 
GAGAAGCTGC TGCTGGCTTG GACTGCGGTG GGGCTGCCCC TCCAGGATGT GTCTGTGGCT 
GCCTGCAATT TCTGTCGCCG TCCTGTACAC TTTGAGCTCA TGAGTGAGTG GGAACGTTCC 
TACTTCGGGA ACATGGGGCC CCAGTATGTC ACCACCTATG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001033057.1
CDS	174..1898
Translation	MRGGEVALGWNKKKRLFCEDFRFKMVTAAMLLQCCPVLARGPTSLLGKVVKTHQFLFGIGRCPILATQGPNCSQIHLKATKAGGDSPSWAKGHCPFMLSELQDGKSKIVQKAAPEVQEDVKAFKTGNYVFSYDQFFRDKIMEKKQDHTYRVFKTVNRWADAYPFAQHFSEASVASKDVSVWCSNDYLGMSRHPQVLQATQETLQRHGAGAGGTRNISGTSKFHVELEQELAELHQKDSALLFSSCFVANDSTLFTLAKILPGCEIYSDAGNHASMIQGIRNSGAAKFVFRHNDPDHLKKLLEKSNPKIPKIVAFETVHSMDGAICPLEELCDVSHQYGALTFVDEVHAVGLYGSRGAGIGERDGIMHKIDIISGTLGKAFGCVGGYIASTRDLVDMVRSYAAGFIFTTSLPPMVLSGALESVRLLKGEEGQALRRAHQRNVKHMRQLLMDRGLPVIPCPSHIIPIRVGNAALNSKLCDLLLSKHGIYVQAINYPTVPRGEELLRLAPSPHHSPQMMEDFVEKLLLAWTAVGLPLQDVSVAACNFCRRPVHFELMSEWERSYFGNMGPQYVTTYA

Target ORF information:

RefSeq Version	NM_001037968.3
Organism	Homo sapiens(human)
Definition	Homo sapiens 5'-aminolevulinate synthase 2 (ALAS2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001037968.3

ORF Insert Sequence:

ATGAGAGGGG GAGAGGTTGC TCTGGGATGG AACAAGAAAA AGAGGTTGTT TTGTGAGGAC 
TTTAGGTTCA AGATGGTGAC TGCAGCCATG CTGCTACAGT GCTGCCCAGT GCTTGCCCGG 
GGCCCCACAA GCCTCCTAGG CAAGGTGGTT AAGACTCACC AGTTCCTGTT TGGTATTGGA 
CGCTGTCCCA TCCTGGCTAC CCAAGGACCA AACTGTTCTC AAATCCACCT TAAGGCAACA 
AAGGCTGGAG GAGATTCTCC ATCTTGGGCG AAGGGCCACT GTCCCTTCAT GCTGTCGGAA 
CTCCAGGATG GGAAGAGCAA GATTGTGCAG AAGGCAGCCC CAGAAGTCCA GGAAGATGTG 
AAGGCTTTCA AGACAGGAAA CTATGTCTTC AGTTATGACC AGTTTTTCAG GGACAAGATC 
ATGGAGAAGA AACAGGATCA CACCTACCGT GTGTTCAAGA CTGTGAACCG CTGGGCTGAT 
GCATATCCCT TTGCCCAACA TTTCTCTGAG GCATCTGTGG CCTCAAAGGA TGTGTCCGTC 
TGGTGTAGTA ATGATTACCT GGGCATGAGC CGACACCCTC AGGTCTTGCA AGCCACACAG 
GAGACCCTGC AGCGTCATGG TGCTGGAGCT GGTGGCACCC GCAACATCTC AGGCACCAGT 
AAGTTTCATG TGGAGCTTGA GCAGGAGCTG GCTGAGCTGC ACCAGAAGGA CTCAGCCCTG 
CTCTTCTCCT CCTGCTTTGT TGCCAATGAC TCTACTCTCT TCACCTTGGC CAAGATCCTG 
CCAGGGTGCG AGATTTACTC AGACGCAGGC AACCATGCTT CCATGATCCA AGGTATCCGT 
AACAGTGGAG CAGCCAAGTT TGTCTTCAGG CACAATGACC CTGACCACCT AAAGAAACTT 
CTAGAGAAGT CTAACCCTAA GATACCCAAA ATTGTGGCCT TTGAGACTGT CCACTCCATG 
GATGGTGCCA TCTGTCCCCT CGAGGAGTTG TGTGATGTGT CCCACCAGTA TGGGGCCCTG 
ACCTTCGTGG ATGAGGTCCA TGCTGTAGGA CTGTATGGGT CCCGGGGCGC TGGGATTGGG 
GAGCGTGATG GAATTATGCA TAAGATTGAC ATCATCTCTG GAACTCTTGG CAAGGCCTTT 
GGCTGTGTGG GCGGCTACAT TGCCAGCACC CGTGACTTGG TGGACATGGT GCGCTCCTAT 
GCTGCAGGCT TCATCTTTAC CACTTCTCTG CCCCCCATGG TGCTCTCTGG AGCTCTAGAA 
TCTGTGCGGC TGCTCAAGGG AGAGGAGGGC CAAGCCCTGA GGCGAGCCCA CCAGCGCAAT 
GTCAAGCACA TGCGCCAGCT ACTCATGGAC AGGGGCCTTC CTGTCATCCC CTGCCCCAGC 
CACATCATCC CCATCCGGGT GGGCAATGCA GCACTCAACA GCAAGCTCTG TGATCTCCTG 
CTCTCCAAGC ATGGCATCTA TGTGCAGGCC ATCAACTACC CAACTGTCCC CCGGGGTGAA 
GAGCTCCTGC GCTTGGCACC CTCCCCCCAC CACAGCCCTC AGATGATGGA AGATTTTGTG 
GAGAAGCTGC TGCTGGCTTG GACTGCGGTG GGGCTGCCCC TCCAGGATGT GTCTGTGGCT 
GCCTGCAATT TCTGTCGCCG TCCTGTACAC TTTGAGCTCA TGAGTGAGTG GGAACGTTCC 
TACTTCGGGA ACATGGGGCC CCAGTATGTC ACCACCTATG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALAS2 ( NM_001037968.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001033057.1 Homo sapiens 5'-aminolevulinate synthase 2 (ALAS2), transcript variant 3, mRNA.

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CloneID	OHu21664
Clone ID Related Accession (Same CDS sequence)	NM_001037968.3 , NM_001037968.4
Accession Version	NM_001037968.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1725bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	5-aminolevulinate synthase, erythroid-specific, mitochondrial isoform c precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP233279.1, AL020991.1 and BQ182291.1. On May 31, 2019 this sequence version replaced NM_001037968.3. Transcript Variant: This variant (3) differs in the 5' UTR, has an additional 5' exon resulting in an alternate start codon, and lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. The resulting isoform (c) is shorter but has a longer and distinct N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2149004 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGAGGGG GAGAGGTTGC TCTGGGATGG AACAAGAAAA AGAGGTTGTT TTGTGAGGAC 
TTTAGGTTCA AGATGGTGAC TGCAGCCATG CTGCTACAGT GCTGCCCAGT GCTTGCCCGG 
GGCCCCACAA GCCTCCTAGG CAAGGTGGTT AAGACTCACC AGTTCCTGTT TGGTATTGGA 
CGCTGTCCCA TCCTGGCTAC CCAAGGACCA AACTGTTCTC AAATCCACCT TAAGGCAACA 
AAGGCTGGAG GAGATTCTCC ATCTTGGGCG AAGGGCCACT GTCCCTTCAT GCTGTCGGAA 
CTCCAGGATG GGAAGAGCAA GATTGTGCAG AAGGCAGCCC CAGAAGTCCA GGAAGATGTG 
AAGGCTTTCA AGACAGGAAA CTATGTCTTC AGTTATGACC AGTTTTTCAG GGACAAGATC 
ATGGAGAAGA AACAGGATCA CACCTACCGT GTGTTCAAGA CTGTGAACCG CTGGGCTGAT 
GCATATCCCT TTGCCCAACA TTTCTCTGAG GCATCTGTGG CCTCAAAGGA TGTGTCCGTC 
TGGTGTAGTA ATGATTACCT GGGCATGAGC CGACACCCTC AGGTCTTGCA AGCCACACAG 
GAGACCCTGC AGCGTCATGG TGCTGGAGCT GGTGGCACCC GCAACATCTC AGGCACCAGT 
AAGTTTCATG TGGAGCTTGA GCAGGAGCTG GCTGAGCTGC ACCAGAAGGA CTCAGCCCTG 
CTCTTCTCCT CCTGCTTTGT TGCCAATGAC TCTACTCTCT TCACCTTGGC CAAGATCCTG 
CCAGGGTGCG AGATTTACTC AGACGCAGGC AACCATGCTT CCATGATCCA AGGTATCCGT 
AACAGTGGAG CAGCCAAGTT TGTCTTCAGG CACAATGACC CTGACCACCT AAAGAAACTT 
CTAGAGAAGT CTAACCCTAA GATACCCAAA ATTGTGGCCT TTGAGACTGT CCACTCCATG 
GATGGTGCCA TCTGTCCCCT CGAGGAGTTG TGTGATGTGT CCCACCAGTA TGGGGCCCTG 
ACCTTCGTGG ATGAGGTCCA TGCTGTAGGA CTGTATGGGT CCCGGGGCGC TGGGATTGGG 
GAGCGTGATG GAATTATGCA TAAGATTGAC ATCATCTCTG GAACTCTTGG CAAGGCCTTT 
GGCTGTGTGG GCGGCTACAT TGCCAGCACC CGTGACTTGG TGGACATGGT GCGCTCCTAT 
GCTGCAGGCT TCATCTTTAC CACTTCTCTG CCCCCCATGG TGCTCTCTGG AGCTCTAGAA 
TCTGTGCGGC TGCTCAAGGG AGAGGAGGGC CAAGCCCTGA GGCGAGCCCA CCAGCGCAAT 
GTCAAGCACA TGCGCCAGCT ACTCATGGAC AGGGGCCTTC CTGTCATCCC CTGCCCCAGC 
CACATCATCC CCATCCGGGT GGGCAATGCA GCACTCAACA GCAAGCTCTG TGATCTCCTG 
CTCTCCAAGC ATGGCATCTA TGTGCAGGCC ATCAACTACC CAACTGTCCC CCGGGGTGAA 
GAGCTCCTGC GCTTGGCACC CTCCCCCCAC CACAGCCCTC AGATGATGGA AGATTTTGTG 
GAGAAGCTGC TGCTGGCTTG GACTGCGGTG GGGCTGCCCC TCCAGGATGT GTCTGTGGCT 
GCCTGCAATT TCTGTCGCCG TCCTGTACAC TTTGAGCTCA TGAGTGAGTG GGAACGTTCC 
TACTTCGGGA ACATGGGGCC CCAGTATGTC ACCACCTATG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001033057.1
CDS	87..1811
Translation	MRGGEVALGWNKKKRLFCEDFRFKMVTAAMLLQCCPVLARGPTSLLGKVVKTHQFLFGIGRCPILATQGPNCSQIHLKATKAGGDSPSWAKGHCPFMLSELQDGKSKIVQKAAPEVQEDVKAFKTGNYVFSYDQFFRDKIMEKKQDHTYRVFKTVNRWADAYPFAQHFSEASVASKDVSVWCSNDYLGMSRHPQVLQATQETLQRHGAGAGGTRNISGTSKFHVELEQELAELHQKDSALLFSSCFVANDSTLFTLAKILPGCEIYSDAGNHASMIQGIRNSGAAKFVFRHNDPDHLKKLLEKSNPKIPKIVAFETVHSMDGAICPLEELCDVSHQYGALTFVDEVHAVGLYGSRGAGIGERDGIMHKIDIISGTLGKAFGCVGGYIASTRDLVDMVRSYAAGFIFTTSLPPMVLSGALESVRLLKGEEGQALRRAHQRNVKHMRQLLMDRGLPVIPCPSHIIPIRVGNAALNSKLCDLLLSKHGIYVQAINYPTVPRGEELLRLAPSPHHSPQMMEDFVEKLLLAWTAVGLPLQDVSVAACNFCRRPVHFELMSEWERSYFGNMGPQYVTTYA

Target ORF information:

RefSeq Version	NM_001037968.4
Organism	Homo sapiens(human)
Definition	Homo sapiens 5'-aminolevulinate synthase 2 (ALAS2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001037968.4

ORF Insert Sequence:

ATGAGAGGGG GAGAGGTTGC TCTGGGATGG AACAAGAAAA AGAGGTTGTT TTGTGAGGAC 
TTTAGGTTCA AGATGGTGAC TGCAGCCATG CTGCTACAGT GCTGCCCAGT GCTTGCCCGG 
GGCCCCACAA GCCTCCTAGG CAAGGTGGTT AAGACTCACC AGTTCCTGTT TGGTATTGGA 
CGCTGTCCCA TCCTGGCTAC CCAAGGACCA AACTGTTCTC AAATCCACCT TAAGGCAACA 
AAGGCTGGAG GAGATTCTCC ATCTTGGGCG AAGGGCCACT GTCCCTTCAT GCTGTCGGAA 
CTCCAGGATG GGAAGAGCAA GATTGTGCAG AAGGCAGCCC CAGAAGTCCA GGAAGATGTG 
AAGGCTTTCA AGACAGGAAA CTATGTCTTC AGTTATGACC AGTTTTTCAG GGACAAGATC 
ATGGAGAAGA AACAGGATCA CACCTACCGT GTGTTCAAGA CTGTGAACCG CTGGGCTGAT 
GCATATCCCT TTGCCCAACA TTTCTCTGAG GCATCTGTGG CCTCAAAGGA TGTGTCCGTC 
TGGTGTAGTA ATGATTACCT GGGCATGAGC CGACACCCTC AGGTCTTGCA AGCCACACAG 
GAGACCCTGC AGCGTCATGG TGCTGGAGCT GGTGGCACCC GCAACATCTC AGGCACCAGT 
AAGTTTCATG TGGAGCTTGA GCAGGAGCTG GCTGAGCTGC ACCAGAAGGA CTCAGCCCTG 
CTCTTCTCCT CCTGCTTTGT TGCCAATGAC TCTACTCTCT TCACCTTGGC CAAGATCCTG 
CCAGGGTGCG AGATTTACTC AGACGCAGGC AACCATGCTT CCATGATCCA AGGTATCCGT 
AACAGTGGAG CAGCCAAGTT TGTCTTCAGG CACAATGACC CTGACCACCT AAAGAAACTT 
CTAGAGAAGT CTAACCCTAA GATACCCAAA ATTGTGGCCT TTGAGACTGT CCACTCCATG 
GATGGTGCCA TCTGTCCCCT CGAGGAGTTG TGTGATGTGT CCCACCAGTA TGGGGCCCTG 
ACCTTCGTGG ATGAGGTCCA TGCTGTAGGA CTGTATGGGT CCCGGGGCGC TGGGATTGGG 
GAGCGTGATG GAATTATGCA TAAGATTGAC ATCATCTCTG GAACTCTTGG CAAGGCCTTT 
GGCTGTGTGG GCGGCTACAT TGCCAGCACC CGTGACTTGG TGGACATGGT GCGCTCCTAT 
GCTGCAGGCT TCATCTTTAC CACTTCTCTG CCCCCCATGG TGCTCTCTGG AGCTCTAGAA 
TCTGTGCGGC TGCTCAAGGG AGAGGAGGGC CAAGCCCTGA GGCGAGCCCA CCAGCGCAAT 
GTCAAGCACA TGCGCCAGCT ACTCATGGAC AGGGGCCTTC CTGTCATCCC CTGCCCCAGC 
CACATCATCC CCATCCGGGT GGGCAATGCA GCACTCAACA GCAAGCTCTG TGATCTCCTG 
CTCTCCAAGC ATGGCATCTA TGTGCAGGCC ATCAACTACC CAACTGTCCC CCGGGGTGAA 
GAGCTCCTGC GCTTGGCACC CTCCCCCCAC CACAGCCCTC AGATGATGGA AGATTTTGTG 
GAGAAGCTGC TGCTGGCTTG GACTGCGGTG GGGCTGCCCC TCCAGGATGT GTCTGTGGCT 
GCCTGCAATT TCTGTCGCCG TCCTGTACAC TTTGAGCTCA TGAGTGAGTG GGAACGTTCC 
TACTTCGGGA ACATGGGGCC CCAGTATGTC ACCACCTATG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALAS2 ( XM_005261995.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005262052.1 Homo sapiens 5'-aminolevulinate synthase 2 (ALAS2), transcript variant X1, mRNA.

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CloneID	OHu54081
Clone ID Related Accession (Same CDS sequence)	XM_005261995.3
Accession Version	XM_005261995.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1836bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	5-aminolevulinate synthase, erythroid-specific, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005261995.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGGGG GAGAGGTTGC TCTGGGATGG AACAAGAAAA AGAGGTTGTT TTGTGAGGAC 
TTTAGGTTCA AGATGGTGAC TGCAGCCATG CTGCTACAGT GCTGCCCAGT GCTTGCCCGG 
GGCCCCACAA GCCTCCTAGG CAAGGTGGTT AAGACTCACC AGTTCCTGTT TGGTATTGGA 
CGCTGTCCCA TCCTGGCTAC CCAAGGACCA AACTGTTCTC AAATCCACCT TAAGGCAACA 
AAGGCTGGAG GAGATTCTCC ATCTTGGGCG AAGGGCCACT GTCCCTTCAT GCTGTCGGAA 
CTCCAGGATG GGAAGAGCAA GATTGTGCAG AAGGCAGCCC CAGAAGTCCA GGAAGATGTG 
AAGGCTTTCA AGACAGATCT GCCTAGCTCC CTGGTCTCAG TCAGCCTAAG GAAGCCATTT 
TCCGGTCCCC AGGAGCAGGA GCAGATCTCT GGGAAGGTCA CACACCTGAT TCAGAACAAT 
ATGCCTGGAA ACTATGTCTT CAGTTATGAC CAGTTTTTCA GGGACAAGAT CATGGAGAAG 
AAACAGGATC ACACCTACCG TGTGTTCAAG ACTGTGAACC GCTGGGCTGA TGCATATCCC 
TTTGCCCAAC ATTTCTCTGA GGCATCTGTG GCCTCAAAGG ATGTGTCCGT CTGGTGTAGT 
AATGATTACC TGGGCATGAG CCGACACCCT CAGGTCTTGC AAGCCACACA GGAGACCCTG 
CAGCGTCATG GTGCTGGAGC TGGTGGCACC CGCAACATCT CAGGCACCAG TAAGTTTCAT 
GTGGAGCTTG AGCAGGAGCT GGCTGAGCTG CACCAGAAGG ACTCAGCCCT GCTCTTCTCC 
TCCTGCTTTG TTGCCAATGA CTCTACTCTC TTCACCTTGG CCAAGATCCT GCCAGGGTGC 
GAGATTTACT CAGACGCAGG CAACCATGCT TCCATGATCC AAGGTATCCG TAACAGTGGA 
GCAGCCAAGT TTGTCTTCAG GCACAATGAC CCTGACCACC TAAAGAAACT TCTAGAGAAG 
TCTAACCCTA AGATACCCAA AATTGTGGCC TTTGAGACTG TCCACTCCAT GGATGGTGCC 
ATCTGTCCCC TCGAGGAGTT GTGTGATGTG TCCCACCAGT ATGGGGCCCT GACCTTCGTG 
GATGAGGTCC ATGCTGTAGG ACTGTATGGG TCCCGGGGCG CTGGGATTGG GGAGCGTGAT 
GGAATTATGC ATAAGATTGA CATCATCTCT GGAACTCTTG GCAAGGCCTT TGGCTGTGTG 
GGCGGCTACA TTGCCAGCAC CCGTGACTTG GTGGACATGG TGCGCTCCTA TGCTGCAGGC 
TTCATCTTTA CCACTTCTCT GCCCCCCATG GTGCTCTCTG GAGCTCTAGA ATCTGTGCGG 
CTGCTCAAGG GAGAGGAGGG CCAAGCCCTG AGGCGAGCCC ACCAGCGCAA TGTCAAGCAC 
ATGCGCCAGC TACTCATGGA CAGGGGCCTT CCTGTCATCC CCTGCCCCAG CCACATCATC 
CCCATCCGGG TGGGCAATGC AGCACTCAAC AGCAAGCTCT GTGATCTCCT GCTCTCCAAG 
CATGGCATCT ATGTGCAGGC CATCAACTAC CCAACTGTCC CCCGGGGTGA AGAGCTCCTG 
CGCTTGGCAC CCTCCCCCCA CCACAGCCCT CAGATGATGG AAGATTTTGT GGAGAAGCTG 
CTGCTGGCTT GGACTGCGGT GGGGCTGCCC CTCCAGGATG TGTCTGTGGC TGCCTGCAAT 
TTCTGTCGCC GTCCTGTACA CTTTGAGCTC ATGAGTGAGT GGGAACGTTC CTACTTCGGG 
AACATGGGGC CCCAGTATGT CACCACCTAT GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005262052.1
CDS	87..1922
Translation	MRGGEVALGWNKKKRLFCEDFRFKMVTAAMLLQCCPVLARGPTSLLGKVVKTHQFLFGIGRCPILATQGPNCSQIHLKATKAGGDSPSWAKGHCPFMLSELQDGKSKIVQKAAPEVQEDVKAFKTDLPSSLVSVSLRKPFSGPQEQEQISGKVTHLIQNNMPGNYVFSYDQFFRDKIMEKKQDHTYRVFKTVNRWADAYPFAQHFSEASVASKDVSVWCSNDYLGMSRHPQVLQATQETLQRHGAGAGGTRNISGTSKFHVELEQELAELHQKDSALLFSSCFVANDSTLFTLAKILPGCEIYSDAGNHASMIQGIRNSGAAKFVFRHNDPDHLKKLLEKSNPKIPKIVAFETVHSMDGAICPLEELCDVSHQYGALTFVDEVHAVGLYGSRGAGIGERDGIMHKIDIISGTLGKAFGCVGGYIASTRDLVDMVRSYAAGFIFTTSLPPMVLSGALESVRLLKGEEGQALRRAHQRNVKHMRQLLMDRGLPVIPCPSHIIPIRVGNAALNSKLCDLLLSKHGIYVQAINYPTVPRGEELLRLAPSPHHSPQMMEDFVEKLLLAWTAVGLPLQDVSVAACNFCRRPVHFELMSEWERSYFGNMGPQYVTTYA

Target ORF information:

RefSeq Version	XM_005261995.3
Organism	Homo sapiens(human)
Definition	Homo sapiens 5'-aminolevulinate synthase 2 (ALAS2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005261995.3

ORF Insert Sequence:

ATGAGAGGGG GAGAGGTTGC TCTGGGATGG AACAAGAAAA AGAGGTTGTT TTGTGAGGAC 
TTTAGGTTCA AGATGGTGAC TGCAGCCATG CTGCTACAGT GCTGCCCAGT GCTTGCCCGG 
GGCCCCACAA GCCTCCTAGG CAAGGTGGTT AAGACTCACC AGTTCCTGTT TGGTATTGGA 
CGCTGTCCCA TCCTGGCTAC CCAAGGACCA AACTGTTCTC AAATCCACCT TAAGGCAACA 
AAGGCTGGAG GAGATTCTCC ATCTTGGGCG AAGGGCCACT GTCCCTTCAT GCTGTCGGAA 
CTCCAGGATG GGAAGAGCAA GATTGTGCAG AAGGCAGCCC CAGAAGTCCA GGAAGATGTG 
AAGGCTTTCA AGACAGATCT GCCTAGCTCC CTGGTCTCAG TCAGCCTAAG GAAGCCATTT 
TCCGGTCCCC AGGAGCAGGA GCAGATCTCT GGGAAGGTCA CACACCTGAT TCAGAACAAT 
ATGCCTGGAA ACTATGTCTT CAGTTATGAC CAGTTTTTCA GGGACAAGAT CATGGAGAAG 
AAACAGGATC ACACCTACCG TGTGTTCAAG ACTGTGAACC GCTGGGCTGA TGCATATCCC 
TTTGCCCAAC ATTTCTCTGA GGCATCTGTG GCCTCAAAGG ATGTGTCCGT CTGGTGTAGT 
AATGATTACC TGGGCATGAG CCGACACCCT CAGGTCTTGC AAGCCACACA GGAGACCCTG 
CAGCGTCATG GTGCTGGAGC TGGTGGCACC CGCAACATCT CAGGCACCAG TAAGTTTCAT 
GTGGAGCTTG AGCAGGAGCT GGCTGAGCTG CACCAGAAGG ACTCAGCCCT GCTCTTCTCC 
TCCTGCTTTG TTGCCAATGA CTCTACTCTC TTCACCTTGG CCAAGATCCT GCCAGGGTGC 
GAGATTTACT CAGACGCAGG CAACCATGCT TCCATGATCC AAGGTATCCG TAACAGTGGA 
GCAGCCAAGT TTGTCTTCAG GCACAATGAC CCTGACCACC TAAAGAAACT TCTAGAGAAG 
TCTAACCCTA AGATACCCAA AATTGTGGCC TTTGAGACTG TCCACTCCAT GGATGGTGCC 
ATCTGTCCCC TCGAGGAGTT GTGTGATGTG TCCCACCAGT ATGGGGCCCT GACCTTCGTG 
GATGAGGTCC ATGCTGTAGG ACTGTATGGG TCCCGGGGCG CTGGGATTGG GGAGCGTGAT 
GGAATTATGC ATAAGATTGA CATCATCTCT GGAACTCTTG GCAAGGCCTT TGGCTGTGTG 
GGCGGCTACA TTGCCAGCAC CCGTGACTTG GTGGACATGG TGCGCTCCTA TGCTGCAGGC 
TTCATCTTTA CCACTTCTCT GCCCCCCATG GTGCTCTCTG GAGCTCTAGA ATCTGTGCGG 
CTGCTCAAGG GAGAGGAGGG CCAAGCCCTG AGGCGAGCCC ACCAGCGCAA TGTCAAGCAC 
ATGCGCCAGC TACTCATGGA CAGGGGCCTT CCTGTCATCC CCTGCCCCAG CCACATCATC 
CCCATCCGGG TGGGCAATGC AGCACTCAAC AGCAAGCTCT GTGATCTCCT GCTCTCCAAG 
CATGGCATCT ATGTGCAGGC CATCAACTAC CCAACTGTCC CCCGGGGTGA AGAGCTCCTG 
CGCTTGGCAC CCTCCCCCCA CCACAGCCCT CAGATGATGG AAGATTTTGT GGAGAAGCTG 
CTGCTGGCTT GGACTGCGGT GGGGCTGCCC CTCCAGGATG TGTCTGTGGC TGCCTGCAAT 
TTCTGTCGCC GTCCTGTACA CTTTGAGCTC ATGAGTGAGT GGGAACGTTC CTACTTCGGG 
AACATGGGGC CCCAGTATGT CACCACCTAT GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALAS2 ( XM_017029354.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884843.1 Homo sapiens 5'-aminolevulinate synthase 2 (ALAS2), transcript variant X2, mRNA.

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CloneID	OHu99203
Clone ID Related Accession (Same CDS sequence)	XM_017029354.1
Accession Version	XM_017029354.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	5-aminolevulinate synthase, erythroid-specific, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGGGG GAGAGGTTGC TCTGGGATGG AACAAGAAAA AGAGGTTGTT TTGTGAGGAC 
TTTAGGTTCA AGATGGTGAC TGCAGCCATG CTGCTACAGT GCTGCCCAGT GCTTGCCCGG 
GGCCCCACAA GCCTCCTAGG CAAGGTGGTT AAGACTCACC AGTTCCTGTT TGGTATTGGA 
CGCTGTCCCA TCCTGGCTAC CCAAGGACCA AACTGTTCTC AAATCCACCT TAAGGCAACA 
AAGGCTGGAG GAGATTCTCC ATCTTGGGCG AAGGGCCACT GTCCCTTCAT GCTGTCGGAA 
CTCCAGGATG GGAAGAGCAA GATTGTGCAG AAGGCAGCCC CAGAAGTCCA GGAAGATGTG 
AAGGCTTTCA AGACAGATCT GCCTAGCTCC CTGGTCTCAG TCAGCCTAAG GAAGCCATTT 
TCCGGTCCCC AGGAGCAGGA GCAGATCTCT GGGAAGGTCA CACACCTGAT TCAGAACAAT 
ATGCCTGGAA ACTATGTCTT CAGTTATGAC CAGTTTTTCA GGGACAAGAT CATGGAGAAG 
AAACAGGATC ACACCTACCG TGTGTTCAAG ACTGTGAACC GCTGGGCTGA TGCATATCCC 
TTTGCCCAAC ATTTCTCTGA GGCATCTGTG GCCTCAAAGG ATGTGTCCGT CTGGTGTAGT 
AATGATTACC TGGGCATGAG CCGACACCCT CAGGTCTTGC AAGCCACACA GGAGACCCTG 
CAGCGTCATG GTGCTGGAGC TGGTGGCACC CGCAACATCT CAGGCACCAG TAAGTTTCAT 
GTGGAGCTTG AGCAGGAGCT GGCTGAGCTG CACCAGAAGG ACTCAGCCCT GCTCTTCTCC 
TCCTGCTTTG TTGCCAATGA CTCTACTCTC TTCACCTTGG CCAAGATCCT GCCAGGGTGC 
GAGATTTACT CAGACGCAGG CAACCATGCT TCCATGATCC AAGGTATCCG TAACAGTGGA 
GCAGCCAAGT TTGTCTTCAG GCACAATGAC CCTGACCACC TAAAGAAACT TCTAGAGAAG 
TCTAACCCTA AGATACCCAA AATTGTGGCC TTTGAGACTG TCCACTCCAT GGATGGTGCC 
ATCTGTCCCC TCGAGGAGTT GTGTGATGTG TCCCACCAGT ATGGGGCCCT GACCTTCGTG 
GATGAGGTCC ATGCTGTAGG ACTGTATGGG TCCCGGGGCG CTGGGATTGG GGAGCGTGAT 
GGAATTATGC ATAAGATTGA CATCATCTCT GGAACTCTTG GCAAGGCCTT TGGCTGTGTG 
GGCGGCTACA TTGCCAGCAC CCGTGACTTG GTGGACATGG TGCGCTCCTA TGCTGCAGGC 
TTCATCTTTA CCACTTCTCT GCCCCCCATG GTGCTCTCTG GAGCTCTAGA ATCTGTGCGG 
CTGCTCAAGG GAGAGGAGGG CCAAGCCCTG AGGCGAGCCC ACCAGCGCAA TGTCAAGCAC 
ATGCGCCAGC TACTCATGGA CAGGGGCCTT CCTGTCATCC CCTGCCCCAG CCACATCATC 
CCCATCCGGG TGGGCAATGC AGCACTCAAC AGCAAGCTCT GTGATCTCCT GCTCTCCAAG 
CATGGCATCT ATGTGCAGGC CATCAACTAC CCAACTGTCC CCCGGGGTGA AGAGCTCCTG 
CGCTTGGCAC CCTCCCCCCA CCACAGCCCT CAGATGATGG AAGATTTTGT GGAGAAGCTG 
AGAGGACAGA GACCACTGAG TTTACAAAAT ATTTCTGGAA CATCTAATGT GTGCCAGCAC 
CTATACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884843.1
CDS	88..1836
Translation	MRGGEVALGWNKKKRLFCEDFRFKMVTAAMLLQCCPVLARGPTSLLGKVVKTHQFLFGIGRCPILATQGPNCSQIHLKATKAGGDSPSWAKGHCPFMLSELQDGKSKIVQKAAPEVQEDVKAFKTDLPSSLVSVSLRKPFSGPQEQEQISGKVTHLIQNNMPGNYVFSYDQFFRDKIMEKKQDHTYRVFKTVNRWADAYPFAQHFSEASVASKDVSVWCSNDYLGMSRHPQVLQATQETLQRHGAGAGGTRNISGTSKFHVELEQELAELHQKDSALLFSSCFVANDSTLFTLAKILPGCEIYSDAGNHASMIQGIRNSGAAKFVFRHNDPDHLKKLLEKSNPKIPKIVAFETVHSMDGAICPLEELCDVSHQYGALTFVDEVHAVGLYGSRGAGIGERDGIMHKIDIISGTLGKAFGCVGGYIASTRDLVDMVRSYAAGFIFTTSLPPMVLSGALESVRLLKGEEGQALRRAHQRNVKHMRQLLMDRGLPVIPCPSHIIPIRVGNAALNSKLCDLLLSKHGIYVQAINYPTVPRGEELLRLAPSPHHSPQMMEDFVEKLRGQRPLSLQNISGTSNVCQHLY

Target ORF information:

RefSeq Version	XM_017029354.1
Organism	Homo sapiens(human)
Definition	Homo sapiens 5'-aminolevulinate synthase 2 (ALAS2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029354.1

ORF Insert Sequence:

ATGAGAGGGG GAGAGGTTGC TCTGGGATGG AACAAGAAAA AGAGGTTGTT TTGTGAGGAC 
TTTAGGTTCA AGATGGTGAC TGCAGCCATG CTGCTACAGT GCTGCCCAGT GCTTGCCCGG 
GGCCCCACAA GCCTCCTAGG CAAGGTGGTT AAGACTCACC AGTTCCTGTT TGGTATTGGA 
CGCTGTCCCA TCCTGGCTAC CCAAGGACCA AACTGTTCTC AAATCCACCT TAAGGCAACA 
AAGGCTGGAG GAGATTCTCC ATCTTGGGCG AAGGGCCACT GTCCCTTCAT GCTGTCGGAA 
CTCCAGGATG GGAAGAGCAA GATTGTGCAG AAGGCAGCCC CAGAAGTCCA GGAAGATGTG 
AAGGCTTTCA AGACAGATCT GCCTAGCTCC CTGGTCTCAG TCAGCCTAAG GAAGCCATTT 
TCCGGTCCCC AGGAGCAGGA GCAGATCTCT GGGAAGGTCA CACACCTGAT TCAGAACAAT 
ATGCCTGGAA ACTATGTCTT CAGTTATGAC CAGTTTTTCA GGGACAAGAT CATGGAGAAG 
AAACAGGATC ACACCTACCG TGTGTTCAAG ACTGTGAACC GCTGGGCTGA TGCATATCCC 
TTTGCCCAAC ATTTCTCTGA GGCATCTGTG GCCTCAAAGG ATGTGTCCGT CTGGTGTAGT 
AATGATTACC TGGGCATGAG CCGACACCCT CAGGTCTTGC AAGCCACACA GGAGACCCTG 
CAGCGTCATG GTGCTGGAGC TGGTGGCACC CGCAACATCT CAGGCACCAG TAAGTTTCAT 
GTGGAGCTTG AGCAGGAGCT GGCTGAGCTG CACCAGAAGG ACTCAGCCCT GCTCTTCTCC 
TCCTGCTTTG TTGCCAATGA CTCTACTCTC TTCACCTTGG CCAAGATCCT GCCAGGGTGC 
GAGATTTACT CAGACGCAGG CAACCATGCT TCCATGATCC AAGGTATCCG TAACAGTGGA 
GCAGCCAAGT TTGTCTTCAG GCACAATGAC CCTGACCACC TAAAGAAACT TCTAGAGAAG 
TCTAACCCTA AGATACCCAA AATTGTGGCC TTTGAGACTG TCCACTCCAT GGATGGTGCC 
ATCTGTCCCC TCGAGGAGTT GTGTGATGTG TCCCACCAGT ATGGGGCCCT GACCTTCGTG 
GATGAGGTCC ATGCTGTAGG ACTGTATGGG TCCCGGGGCG CTGGGATTGG GGAGCGTGAT 
GGAATTATGC ATAAGATTGA CATCATCTCT GGAACTCTTG GCAAGGCCTT TGGCTGTGTG 
GGCGGCTACA TTGCCAGCAC CCGTGACTTG GTGGACATGG TGCGCTCCTA TGCTGCAGGC 
TTCATCTTTA CCACTTCTCT GCCCCCCATG GTGCTCTCTG GAGCTCTAGA ATCTGTGCGG 
CTGCTCAAGG GAGAGGAGGG CCAAGCCCTG AGGCGAGCCC ACCAGCGCAA TGTCAAGCAC 
ATGCGCCAGC TACTCATGGA CAGGGGCCTT CCTGTCATCC CCTGCCCCAG CCACATCATC 
CCCATCCGGG TGGGCAATGC AGCACTCAAC AGCAAGCTCT GTGATCTCCT GCTCTCCAAG 
CATGGCATCT ATGTGCAGGC CATCAACTAC CCAACTGTCC CCCGGGGTGA AGAGCTCCTG 
CGCTTGGCAC CCTCCCCCCA CCACAGCCCT CAGATGATGG AAGATTTTGT GGAGAAGCTG 
AGAGGACAGA GACCACTGAG TTTACAAAAT ATTTCTGGAA CATCTAATGT GTGCCAGCAC 
CTATACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ALAS2
Entrez Gene ID	212
Full Name	5'-aminolevulinate synthase 2
Synonyms	ALAS-E,ALASE,ANH1,ASB,SIDBA1,XLDPP,XLEPP,XLSA
General protein information	Preferred Names 5'-aminolevulinate synthase 2 Names 5-aminolevulinate synthase, erythroid-specific, mitochondrial 5-aminolevulinic acid synthase 2 aminolevulinate, delta-, synthase 2 delta-ALA synthase 2 delta-ALA synthetase delta-aminolevulinate synthase 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xp11.21
Summary	The product of this gene specifies an erythroid-specific mitochondrially located enzyme. The encoded protein catalyzes the first step in the heme biosynthetic pathway. Defects in this gene cause X-linked pyridoxine-responsive sideroblastic anemia. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008].
Disorder MIM:	301300
Disorder Html:	Anemia, sideroblastic, 1, 300751 (3); Protoporphyria, erythropoietic, X-linked, 300752 (3)

Drosophila melanogaster (fruit fly)	Alas	NP_477281.1
Xenopus tropicalis (tropical clawed frog)	alas2	NP_001006926.1
Canis lupus familiaris (dog)	ALAS2	XP_548619.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003184	XP_312882.5
Saccharomyces cerevisiae (baker's yeast)	HEM1	NP_010518.1
Eremothecium gossypii	AGOS_ABL104C	NP_982843.1
Danio rerio (zebrafish)	alas2	NP_571757.1
Magnaporthe oryzae (rice blast fungus)	MGG_06446	XP_003717121.1
Bos taurus (cattle)	ALAS2	NP_001030275.1
Rattus norvegicus (Norway rat)	Alas2	NP_037329.1
Schizosaccharomyces pombe (fission yeast)	hem1	NP_594388.1
Neurospora crassa	NCU06189	XP_963001.2
Homo sapiens (human)	ALAS2	NP_000023.2
Pan troglodytes (chimpanzee)	ALAS2	XP_528998.2
	ALAS2	XP_002806298.1
Mus musculus (house mouse)	Alas2	NP_033783.1
Kluyveromyces lactis	KLLA0C15059g	XP_452875.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ALAS2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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