WBP2NL cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	WBP2NL
Entrez Gene ID	164684
Full Name	WBP2 N-terminal like
Synonyms	GRAMD7,PAWP
General protein information	Preferred Names WBP2 N-terminal like Names postacrosomal sheath WW domain-binding protein WW domain-binding protein 2-like
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 22 Map Location: 22q13.2
Summary	WBP2NL is a sperm-specific WW domain-binding protein that promotes meiotic resumption and pronuclear development during oocyte fertilization (Wu et al., 2007 [PubMed 17289678]).[supplied by OMIM, Mar 2008].
Disorder MIM:	610981

mRNA and Protein(s)

mRNA	Protein	Name
NM_152613.2	NP_689826.2	postacrosomal sheath WW domain-binding protein
NM_152613.3	NP_689826.2	postacrosomal sheath WW domain-binding protein

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Wbp2nl	NP_083342.1
Rattus norvegicus (Norway rat)	Wbp2nl	XP_001067725.2
	LOC709297	XP_002798421.1
Canis lupus familiaris (dog)	WBP2NL	XP_005625874.1
Bos taurus (cattle)	WBP2NL	NP_001075910.1
Homo sapiens (human)	WBP2NL	NP_689826.2
Pan troglodytes (chimpanzee)	WBP2NL	XP_003317337.1

Gene Ontology
Bibliography

Related articles in PubMed

Is there an association between PAWP/WBP2NL sequence, expression, and distribution in sperm cells and fertilization failures in ICSI cycles?
Freour T, Barragan M, Torra-Massana M, Ferrer-Vaquer A, Vassena R
Molecular reproduction and development(2017 Dec)

Oligoasthenoteratozoospermic (OAT) men display altered phospholipase C ? (PLC?) localization and a lower percentage of sperm cells expressing PLC? and post-acrosomal sheath WW domain-binding protein (PAWP).
Azad N, Nazarian H, Ghaffari Novin M, Masteri Farahani R, Piryaei A, Heidari MH, Abdollahpour Alitappeh M
Bosnian journal of basic medical sciences(2017 Sep)

WBP2NL/PAWP mRNA and protein expression in sperm cells are not related to semen parameters, fertilization rate, or reproductive outcome.
Freour T, Barragan M, Ferrer-Vaquer A, Rodr?guez A, Vassena R
Journal of assisted reproduction and genetics34(6)803-810(2017 Jun)

Status of sperm-born oocyte activating factors (PAWP, PLC?) and sperm chromatin in uncapacitated, capacitated and acrosome-reacted conditions.
Tavalaee M, Parivar K, Shahverdi AH, Ghaedi K, Nasr-Esfahani MH
Human fertility (Cambridge, England)20(2)96-103(2017 Jun)

Expression profile of PLC?, PAWP, and TR-KIT in association with fertilization potential, embryo development, and pregnancy outcomes in globozoospermic candidates for intra-cytoplasmic sperm injection and artificial oocyte activation.
Tavalaee M, Nasr-Esfahani MH
Andrology4(5)850-6(2016 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Taken into account that WBP2NL/PAWP gene and protein levels and distribution did not correlate with fertilization rates, this study questions the interest of WBP2NL/PAWP protein and gene expression analysis in sperm cells as a prognostic factor for the outcome of ICSI cycles
Title: WBP2NL/PAWP mRNA and protein expression in sperm cells are not related to semen parameters, fertilization rate, or reproductive outcome.

High fertilization (56.06%) and pregnancy (41.7%) rates accomplished in this study following ICSI-AOA indicated that expression profiles of PLCzeta, PAWP, and TR-KIT were low in globozoospermic individuals
Title: Expression profile of PLC?, PAWP, and TR-KIT in association with fertilization potential, embryo development, and pregnancy outcomes in globozoospermic candidates for intra-cytoplasmic sperm injection and artificial oocyte activation.

In male infertility, the absence of PLCZ1 alone is sufficient to prevent oocyte activation irrespective of the presence of PAWP.
Title: Homozygous mutation of PLCZ1 leads to defective human oocyte activation and infertility that is not rescued by the WW-binding protein PAWP.

Conclude that human PAWP is neither sufficient nor necessary for the Ca(2+) oscillations that initiate mammalian oocyte activation at fertilization.
Title: Functional disparity between human PAWP and PLC? in the generation of Ca2+ oscillations for oocyte activation.

Data suggest that PAWP (WW domain-binding protein 2-like) from sperm (rather than PLCZ [phospholipase C zeta] from sperm) acts as the 'sperm factor' within the egg at fertilization and is involved in 'egg activation' and embryogenesis. [REVIEW]
Title: PLC? or PAWP: revisiting the putative mammalian sperm factor that triggers egg activation and embryogenesis.

The following WBP2NL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WBP2NL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu12615	NM_152613.3 Latest version!	Homo sapiens WBP2 N-terminal like (WBP2NL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
View Old Accession Versions >>
OHu12615	NM_152613.2	Homo sapiens WBP2 N-terminal like (WBP2NL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

WBP2NL ( NM_152613.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_689826.2 Homo sapiens WBP2 N-terminal like (WBP2NL), mRNA.

Price & Availability↑

CloneID	OHu12615
Clone ID Related Accession (Same CDS sequence)	NM_152613.2 , NM_152613.3
Accession Version	NM_152613.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-09
Organism	Homo sapiens(human)
Product	postacrosomal sheath WW domain-binding protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC022546.1, AK128138.1 and Z99716.4. On Nov 23, 2018 this sequence version replaced NM_152613.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC022546.1, SRR1660803.12835.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000328823.13/ ENSP00000332983.9 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGTGA ATCAGAGCCA CACCGAGAAC CGCCGCGGAG CCCTCATCCC TAACGGTGAA 
AGTCTCTTGA AGCGGTCTCC GAATGTGGAG CTCTCCTTCC CACAGCGATC AGAAGGCTCA 
AATGTCTTTA GTGGTAGAAA GACAGGAACA TTGTTTCTCA CTTCATACCG GGTGATTTTC 
ATAACTTCAT GCTCCATCAG TGATCCCATG TTGTCTTTTA TGATGCCATT TGATCTGATG 
ACGAACCTCA CTGTTGAACA ACCAGTATTT GCTGCAAACT TCATTAAGGG AACTATTCAG 
GCAGCTCCAT ATGGTGGCTG GGAAGGACAA GCTACTTTTA AATTAGTCTT CAGAAATGGA 
GATGCCATTG AATTTGCCCA GTTGATGGTG AAAGCTGCCT CTGCTGCTGC CCGAGGATTT 
CCACTTAGAA CCTTAAATGA CTGGTTCAGC TCTATGGGAA TTTATGTAAT TACTGGGGAA 
GGGAATATGT GCACTCCACA GATGCCTTGT TCAGTTATTG TCTATGGAGC CCCACCTGCA 
GGATATGGAG CCCCACCTCC CGGATACGGA GCCCCACCTG CAGGATATGG AGCCCAACCC 
GTAGGAAATG AAGGCCCGCC TGTGGGATAC AGAGCCTCAC CTGTGCGATA TGGAGCCCCA 
CCTCTTGGAT ACGGAGCCCC ACCTGCAGGA TATGGAGCCC CACCTCTAGG ATATGGAGCC 
CCACCTCTTG GATATGGAAC CCCACCTCTC GGATATGGAG CCCCACCTCT CGGATATGGA 
GCCCCACCTG CAGGAAATGA AGGCCCGCCT GCGGGATACA GAGCCTCACC TGCTGGATCA 
GGAGCCAGGC CTCAGGAATC TACAGCAGCC CAGGCTCCTG AAAACGAGGC TTCTCTTCCC 
TCTGCCTCCT CTTCTCAGGT CCATTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_689826.2
CDS	32..961
Translation	MAVNQSHTENRRGALIPNGESLLKRSPNVELSFPQRSEGSNVFSGRKTGTLFLTSYRVIFITSCSISDPMLSFMMPFDLMTNLTVEQPVFAANFIKGTIQAAPYGGWEGQATFKLVFRNGDAIEFAQLMVKAASAAARGFPLRTLNDWFSSMGIYVITGEGNMCTPQMPCSVIVYGAPPAGYGAPPPGYGAPPAGYGAQPVGNEGPPVGYRASPVRYGAPPLGYGAPPAGYGAPPLGYGAPPLGYGTPPLGYGAPPLGYGAPPAGNEGPPAGYRASPAGSGARPQESTAAQAPENEASLPSASSSQVHS

Target ORF information:

RefSeq Version	NM_152613.3
Organism	Homo sapiens(human)
Definition	Homo sapiens WBP2 N-terminal like (WBP2NL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152613.3

ORF Insert Sequence:

ATGGCGGTGA ATCAGAGCCA CACCGAGAAC CGCCGCGGAG CCCTCATCCC TAACGGTGAA 
AGTCTCTTGA AGCGGTCTCC GAATGTGGAG CTCTCCTTCC CACAGCGATC AGAAGGCTCA 
AATGTCTTTA GTGGTAGAAA GACAGGAACA TTGTTTCTCA CTTCATACCG GGTGATTTTC 
ATAACTTCAT GCTCCATCAG TGATCCCATG TTGTCTTTTA TGATGCCATT TGATCTGATG 
ACGAACCTCA CTGTTGAACA ACCAGTATTT GCTGCAAACT TCATTAAGGG AACTATTCAG 
GCAGCTCCAT ATGGTGGCTG GGAAGGACAA GCTACTTTTA AATTAGTCTT CAGAAATGGA 
GATGCCATTG AATTTGCCCA GTTGATGGTG AAAGCTGCCT CTGCTGCTGC CCGAGGATTT 
CCACTTAGAA CCTTAAATGA CTGGTTCAGC TCTATGGGAA TTTATGTAAT TACTGGGGAA 
GGGAATATGT GCACTCCACA GATGCCTTGT TCAGTTATTG TCTATGGAGC CCCACCTGCA 
GGATATGGAG CCCCACCTCC CGGATACGGA GCCCCACCTG CAGGATATGG AGCCCAACCC 
GTAGGAAATG AAGGCCCGCC TGTGGGATAC AGAGCCTCAC CTGTGCGATA TGGAGCCCCA 
CCTCTTGGAT ACGGAGCCCC ACCTGCAGGA TATGGAGCCC CACCTCTAGG ATATGGAGCC 
CCACCTCTTG GATATGGAAC CCCACCTCTC GGATATGGAG CCCCACCTCT CGGATATGGA 
GCCCCACCTG CAGGAAATGA AGGCCCGCCT GCGGGATACA GAGCCTCACC TGCTGGATCA 
GGAGCCAGGC CTCAGGAATC TACAGCAGCC CAGGCTCCTG AAAACGAGGC TTCTCTTCCC 
TCTGCCTCCT CTTCTCAGGT CCATTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WBP2NL ( NM_152613.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_689826.2 Homo sapiens WBP2 N-terminal like (WBP2NL), mRNA.

Price & Availability↑

CloneID	OHu12615
Clone ID Related Accession (Same CDS sequence)	NM_152613.2 , NM_152613.3
Accession Version	NM_152613.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 930bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	postacrosomal sheath WW domain-binding protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB079211.1, BC022546.1, AK128138.1 and Z99716.4. On Mar 6, 2009 this sequence version replaced NM_152613.1. ##Evidence-Data-START## Transcript exon combination :: BC022546.1, SRR1660803.12835.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGTGA ATCAGAGCCA CACCGAGAAC CGCCGCGGAG CCCTCATCCC TAACGGTGAA 
AGTCTCTTGA AGCGGTCTCC GAATGTGGAG CTCTCCTTCC CACAGCGATC AGAAGGCTCA 
AATGTCTTTA GTGGTAGAAA GACAGGAACA TTGTTTCTCA CTTCATACCG GGTGATTTTC 
ATAACTTCAT GCTCCATCAG TGATCCCATG TTGTCTTTTA TGATGCCATT TGATCTGATG 
ACGAACCTCA CTGTTGAACA ACCAGTATTT GCTGCAAACT TCATTAAGGG AACTATTCAG 
GCAGCTCCAT ATGGTGGCTG GGAAGGACAA GCTACTTTTA AATTAGTCTT CAGAAATGGA 
GATGCCATTG AATTTGCCCA GTTGATGGTG AAAGCTGCCT CTGCTGCTGC CCGAGGATTT 
CCACTTAGAA CCTTAAATGA CTGGTTCAGC TCTATGGGAA TTTATGTAAT TACTGGGGAA 
GGGAATATGT GCACTCCACA GATGCCTTGT TCAGTTATTG TCTATGGAGC CCCACCTGCA 
GGATATGGAG CCCCACCTCC CGGATACGGA GCCCCACCTG CAGGATATGG AGCCCAACCC 
GTAGGAAATG AAGGCCCGCC TGTGGGATAC AGAGCCTCAC CTGTGCGATA TGGAGCCCCA 
CCTCTTGGAT ACGGAGCCCC ACCTGCAGGA TATGGAGCCC CACCTCTAGG ATATGGAGCC 
CCACCTCTTG GATATGGAAC CCCACCTCTC GGATATGGAG CCCCACCTCT CGGATATGGA 
GCCCCACCTG CAGGAAATGA AGGCCCGCCT GCGGGATACA GAGCCTCACC TGCTGGATCA 
GGAGCCAGGC CTCAGGAATC TACAGCAGCC CAGGCTCCTG AAAACGAGGC TTCTCTTCCC 
TCTGCCTCCT CTTCTCAGGT CCATTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_689826.2
CDS	95..1024
Translation	MAVNQSHTENRRGALIPNGESLLKRSPNVELSFPQRSEGSNVFSGRKTGTLFLTSYRVIFITSCSISDPMLSFMMPFDLMTNLTVEQPVFAANFIKGTIQAAPYGGWEGQATFKLVFRNGDAIEFAQLMVKAASAAARGFPLRTLNDWFSSMGIYVITGEGNMCTPQMPCSVIVYGAPPAGYGAPPPGYGAPPAGYGAQPVGNEGPPVGYRASPVRYGAPPLGYGAPPAGYGAPPLGYGAPPLGYGTPPLGYGAPPLGYGAPPAGNEGPPAGYRASPAGSGARPQESTAAQAPENEASLPSASSSQVHS

Target ORF information:

RefSeq Version	NM_152613.2
Organism	Homo sapiens(human)
Definition	Homo sapiens WBP2 N-terminal like (WBP2NL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152613.2

ORF Insert Sequence:

ATGGCGGTGA ATCAGAGCCA CACCGAGAAC CGCCGCGGAG CCCTCATCCC TAACGGTGAA 
AGTCTCTTGA AGCGGTCTCC GAATGTGGAG CTCTCCTTCC CACAGCGATC AGAAGGCTCA 
AATGTCTTTA GTGGTAGAAA GACAGGAACA TTGTTTCTCA CTTCATACCG GGTGATTTTC 
ATAACTTCAT GCTCCATCAG TGATCCCATG TTGTCTTTTA TGATGCCATT TGATCTGATG 
ACGAACCTCA CTGTTGAACA ACCAGTATTT GCTGCAAACT TCATTAAGGG AACTATTCAG 
GCAGCTCCAT ATGGTGGCTG GGAAGGACAA GCTACTTTTA AATTAGTCTT CAGAAATGGA 
GATGCCATTG AATTTGCCCA GTTGATGGTG AAAGCTGCCT CTGCTGCTGC CCGAGGATTT 
CCACTTAGAA CCTTAAATGA CTGGTTCAGC TCTATGGGAA TTTATGTAAT TACTGGGGAA 
GGGAATATGT GCACTCCACA GATGCCTTGT TCAGTTATTG TCTATGGAGC CCCACCTGCA 
GGATATGGAG CCCCACCTCC CGGATACGGA GCCCCACCTG CAGGATATGG AGCCCAACCC 
GTAGGAAATG AAGGCCCGCC TGTGGGATAC AGAGCCTCAC CTGTGCGATA TGGAGCCCCA 
CCTCTTGGAT ACGGAGCCCC ACCTGCAGGA TATGGAGCCC CACCTCTAGG ATATGGAGCC 
CCACCTCTTG GATATGGAAC CCCACCTCTC GGATATGGAG CCCCACCTCT CGGATATGGA 
GCCCCACCTG CAGGAAATGA AGGCCCGCCT GCGGGATACA GAGCCTCACC TGCTGGATCA 
GGAGCCAGGC CTCAGGAATC TACAGCAGCC CAGGCTCCTG AAAACGAGGC TTCTCTTCCC 
TCTGCCTCCT CTTCTCAGGT CCATTCTTAA