AWAT2 cDNA ORF clone, Homo sapiens(Human)

Gene
Clones
gRNAs

Summary

Gene Symbol	AWAT2
Entrez Gene ID	158835
Full Name	acyl-CoA wax alcohol acyltransferase 2
Synonyms	ARAT,DC4,DGAT2L4,MFAT,WS
General protein information	Preferred Names acyl-CoA wax alcohol acyltransferase 2 Names acyl-CoA wax alcohol acyltransferase 2 acyl-CoA retinol O-fatty-acyltransferase diacylglycerol O-acyltransferase 2-like protein 4 diacylglycerol O-acyltransferase candidate 4 long-chain-alcohol O-fatty-acyltransferase 2 multifunctional O-acyltransferase retinol O-fatty-acyltransferase
Gene Type	protein-coding
Organism	Homo sapiens(Human)
Genome	Chromosome: X Map Location: Xq13.1
Summary	This gene encodes an enzyme belonging to the diacylglycerol acyltransferase family. This enzyme produces wax esters by the esterification of long chain (or wax) alcohols with acyl-CoA-derived fatty acids. It functions in lipid metabolism in the skin, mostly in undifferentiated peripheral sebocytes. This enzyme may also have acyl-CoA:retinol acyltransferase activities, where it catalyzes the synthesis of diacylglycerols and retinyl esters. [provided by RefSeq, May 2010].
Disorder MIM:	300925

mRNA and Protein(s)

mRNA	Protein	Name
XM_011530876.2	XP_011529178.1	acyl-CoA wax alcohol acyltransferase 2 isoform X1
NM_001002254.1	NP_001002254.1	acyl-CoA wax alcohol acyltransferase 2
XM_011530876.1	XP_011529178.1	acyl-CoA wax alcohol acyltransferase 2 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	AWAT2	NP_001002254.1
Pan troglodytes (chimpanzee)	AWAT2	XP_529027.1
Bos taurus (cattle)	AWAT2	XP_002700092.3
Mus musculus (house mouse)	Awat2	NP_808414.2
Rattus norvegicus (Norway rat)	Awat2	NP_001178653.1
	AWAT2	XP_001085075.1
Canis lupus familiaris (dog)	AWAT2	XP_549056.2

Gene Ontology
Bibliography

Related articles in PubMed

Identification of the 11-cis-specific retinyl-ester synthase in retinal M?ller cells as multifunctional O-acyltransferase (MFAT).
Kaylor JJ, Cook JD, Makshanoff J, Bischoff N, Yong J, Travis GH
Proceedings of the National Academy of Sciences of the United States of America111(20)7302-7(2014 May)

Comparative genomics and proteomics of vertebrate diacylglycerol acyltransferase (DGAT), acyl CoA wax alcohol acyltransferase (AWAT) and monoacylglycerol acyltransferase (MGAT).
Holmes RS
Comparative biochemistry and physiology. Part D, Genomics & proteomics5(1)45-54(2010 Mar)

A human skin multifunctional O-acyltransferase that catalyzes the synthesis of acylglycerols, waxes, and retinyl esters.
Yen CL, Brown CH 4th, Monetti M, Farese RV Jr
Journal of lipid research46(11)2388-97(2005 Nov)

Identification of two novel human acyl-CoA wax alcohol acyltransferases: members of the diacylglycerol acyltransferase 2 (DGAT2) gene superfamily.
Turkish AR, Henneberry AL, Cromley D, Padamsee M, Oelkers P, Bazzi H, Christiano AM, Billheimer JT, Sturley SL
The Journal of biological chemistry280(15)14755-64(2005 Apr)

Mammalian wax biosynthesis. II. Expression cloning of wax synthase cDNAs encoding a member of the acyltransferase enzyme family.
Cheng JB, Russell DW
The Journal of biological chemistry279(36)37798-807(2004 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

MFAT is a multifunctional acyltransferase that likely plays an important role in lipid metabolism in human skin.
Title: A human skin multifunctional O-acyltransferase that catalyzes the synthesis of acylglycerols, waxes, and retinyl esters.

analysis of AWAT1 and AWAT2 substrate specificity and differentiation-specific expression pattern
Title: Identification of two novel human acyl-CoA wax alcohol acyltransferases: members of the diacylglycerol acyltransferase 2 (DGAT2) gene superfamily.

The following AWAT2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AWAT2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu28300	NM_001002254.1 Latest version!	Homo sapiens acyl-CoA wax alcohol acyltransferase 2 (AWAT2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$139.50-$195.30 $279.00
OHu28300	XM_011530876.2 Latest version!	Homo sapiens acyl-CoA wax alcohol acyltransferase 2 (AWAT2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$139.50-$195.30 $279.00
View Old Accession Versions >>
OHu28300	XM_011530876.1	Homo sapiens acyl-CoA wax alcohol acyltransferase 2 (AWAT2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$139.50-$195.30 $279.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AWAT2 ( NM_001002254.1 ) cDNA ORF clone, Homo sapiens(Human) -> NP_001002254.1 Homo sapiens acyl-CoA wax alcohol acyltransferase 2 (AWAT2), mRNA.

Price & Availability↑

CloneID	OHu28300
Clone ID Related Accession (Same CDS sequence)	XM_011530876.2 , NM_001002254.1 , XM_011530876.1
Accession Version	NM_001002254.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Homo sapiens(human)
Product	acyl-CoA wax alcohol acyltransferase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY605053.1 and BC063698.1. This sequence is a reference standard in the RefSeqGene project. On Aug 30, 2004 this sequence version replaced XM_088683.3. ##Evidence-Data-START## CDS exon combination :: AY605053.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2163623 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000276101.7/ ENSP00000421172.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTCTTGC CCTCTAAGAA GGACCTCAAG ACTGCCCTGG ATGTCTTTGC TGTTTTCCAG 
TGGTCCTTCA GTGCCTTGCT TATCACAACC ACTGTGATTG CTGTCAACCT CTACCTGGTG 
GTGTTCACAC CATACTGGCC TGTCACTGTG CTTATTCTTA CCTGGCTGGC TTTTGACTGG 
AAGACCCCTC AGCGAGGCGG CCGCCGGTTT ACCTGTGTGA GGCACTGGCG CCTGTGGAAA 
CACTACAGCG ATTATTTCCC TCTCAAGCTT CTGAAGACTC ATGACATCTG CCCCAGCCGC 
AACTACATCC TCGTCTGCCA CCCTCATGGG CTCTTTGCCC ATGGATGGTT TGGCCACTTT 
GCCACAGAGG CCTCAGGCTT CTCCAAGATA TTTCCTGGCA TCACCCCTTA CATACTCACA 
CTGGGAGCCT TTTTCTGGAT GCCTTTCCTC AGAGAATATG TAATGTCTAC AGGGGCCTGC 
TCTGTGAGTC GATCCTCCAT TGACTTTCTG CTGACTCATA AAGGCACAGG CAACATGGTC 
ATTGTGGTGA TTGGTGGACT GGCTGAGTGC AGATACAGCC TGCCAGGTTC TTCTACCCTG 
GTGTTGAAGA ACCGGTCTGG CTTTGTGCGC ATGGCCCTTC AGCATGGGGT GCCTCTAATA 
CCTGCCTATG CCTTTGGGGA GACGGACCTC TATGATCAGC ACATTTTCAC TCCTGGTGGC 
TTTGTCAACC GCTTCCAGAA GTGGTTCCAG AGCATGGTAC ACATCTACCC TTGTGCTTTC 
TATGGACGTG GCTTCACCAA GAACTCCTGG GGCCTTCTGC CCTATAGTCG GCCTGTAACC 
ACCATCGTCG GGGAGCCTCT ACCAATGCCC AAGATTGAGA ATCCAAGCCA GGAGATCGTG 
GCTAAATATC ACACACTCTA TATTGATGCC CTACGTAAAC TGTTTGACCA GCATAAGACC 
AAGTTTGGTA TCTCAGAGAC CCAGGAGCTG GAGATAATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001002254.1
CDS	7..1008
Translation	MLLPSKKDLKTALDVFAVFQWSFSALLITTTVIAVNLYLVVFTPYWPVTVLILTWLAFDWKTPQRGGRRFTCVRHWRLWKHYSDYFPLKLLKTHDICPSRNYILVCHPHGLFAHGWFGHFATEASGFSKIFPGITPYILTLGAFFWMPFLREYVMSTGACSVSRSSIDFLLTHKGTGNMVIVVIGGLAECRYSLPGSSTLVLKNRSGFVRMALQHGVPLIPAYAFGETDLYDQHIFTPGGFVNRFQKWFQSMVHIYPCAFYGRGFTKNSWGLLPYSRPVTTIVGEPLPMPKIENPSQEIVAKYHTLYIDALRKLFDQHKTKFGISETQELEII

Target ORF information:

RefSeq Version	NM_001002254.1
Organism	Homo sapiens(human)
Definition	Homo sapiens acyl-CoA wax alcohol acyltransferase 2 (AWAT2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001002254.1

ORF Insert Sequence:

ATGCTCTTGC CCTCTAAGAA GGACCTCAAG ACTGCCCTGG ATGTCTTTGC TGTTTTCCAG 
TGGTCCTTCA GTGCCTTGCT TATCACAACC ACTGTGATTG CTGTCAACCT CTACCTGGTG 
GTGTTCACAC CATACTGGCC TGTCACTGTG CTTATTCTTA CCTGGCTGGC TTTTGACTGG 
AAGACCCCTC AGCGAGGCGG CCGCCGGTTT ACCTGTGTGA GGCACTGGCG CCTGTGGAAA 
CACTACAGCG ATTATTTCCC TCTCAAGCTT CTGAAGACTC ATGACATCTG CCCCAGCCGC 
AACTACATCC TCGTCTGCCA CCCTCATGGG CTCTTTGCCC ATGGATGGTT TGGCCACTTT 
GCCACAGAGG CCTCAGGCTT CTCCAAGATA TTTCCTGGCA TCACCCCTTA CATACTCACA 
CTGGGAGCCT TTTTCTGGAT GCCTTTCCTC AGAGAATATG TAATGTCTAC AGGGGCCTGC 
TCTGTGAGTC GATCCTCCAT TGACTTTCTG CTGACTCATA AAGGCACAGG CAACATGGTC 
ATTGTGGTGA TTGGTGGACT GGCTGAGTGC AGATACAGCC TGCCAGGTTC TTCTACCCTG 
GTGTTGAAGA ACCGGTCTGG CTTTGTGCGC ATGGCCCTTC AGCATGGGGT GCCTCTAATA 
CCTGCCTATG CCTTTGGGGA GACGGACCTC TATGATCAGC ACATTTTCAC TCCTGGTGGC 
TTTGTCAACC GCTTCCAGAA GTGGTTCCAG AGCATGGTAC ACATCTACCC TTGTGCTTTC 
TATGGACGTG GCTTCACCAA GAACTCCTGG GGCCTTCTGC CCTATAGTCG GCCTGTAACC 
ACCATCGTCG GGGAGCCTCT ACCAATGCCC AAGATTGAGA ATCCAAGCCA GGAGATCGTG 
GCTAAATATC ACACACTCTA TATTGATGCC CTACGTAAAC TGTTTGACCA GCATAAGACC 
AAGTTTGGTA TCTCAGAGAC CCAGGAGCTG GAGATAATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AWAT2 ( XM_011530876.2 ) cDNA ORF clone, Homo sapiens(Human) -> XP_011529178.1 Homo sapiens acyl-CoA wax alcohol acyltransferase 2 (AWAT2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu28300
Clone ID Related Accession (Same CDS sequence)	XM_011530876.2 , NM_001002254.1 , XM_011530876.1
Accession Version	XM_011530876.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	acyl-CoA wax alcohol acyltransferase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530876.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTTGC CCTCTAAGAA GGACCTCAAG ACTGCCCTGG ATGTCTTTGC TGTTTTCCAG 
TGGTCCTTCA GTGCCTTGCT TATCACAACC ACTGTGATTG CTGTCAACCT CTACCTGGTG 
GTGTTCACAC CATACTGGCC TGTCACTGTG CTTATTCTTA CCTGGCTGGC TTTTGACTGG 
AAGACCCCTC AGCGAGGCGG CCGCCGGTTT ACCTGTGTGA GGCACTGGCG CCTGTGGAAA 
CACTACAGCG ATTATTTCCC TCTCAAGCTT CTGAAGACTC ATGACATCTG CCCCAGCCGC 
AACTACATCC TCGTCTGCCA CCCTCATGGG CTCTTTGCCC ATGGATGGTT TGGCCACTTT 
GCCACAGAGG CCTCAGGCTT CTCCAAGATA TTTCCTGGCA TCACCCCTTA CATACTCACA 
CTGGGAGCCT TTTTCTGGAT GCCTTTCCTC AGAGAATATG TAATGTCTAC AGGGGCCTGC 
TCTGTGAGTC GATCCTCCAT TGACTTTCTG CTGACTCATA AAGGCACAGG CAACATGGTC 
ATTGTGGTGA TTGGTGGACT GGCTGAGTGC AGATACAGCC TGCCAGGTTC TTCTACCCTG 
GTGTTGAAGA ACCGGTCTGG CTTTGTGCGC ATGGCCCTTC AGCATGGGGT GCCTCTAATA 
CCTGCCTATG CCTTTGGGGA GACGGACCTC TATGATCAGC ACATTTTCAC TCCTGGTGGC 
TTTGTCAACC GCTTCCAGAA GTGGTTCCAG AGCATGGTAC ACATCTACCC TTGTGCTTTC 
TATGGACGTG GCTTCACCAA GAACTCCTGG GGCCTTCTGC CCTATAGTCG GCCTGTAACC 
ACCATCGTCG GGGAGCCTCT ACCAATGCCC AAGATTGAGA ATCCAAGCCA GGAGATCGTG 
GCTAAATATC ACACACTCTA TATTGATGCC CTACGTAAAC TGTTTGACCA GCATAAGACC 
AAGTTTGGTA TCTCAGAGAC CCAGGAGCTG GAGATAATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011529178.1
CDS	39..1040
Translation	MLLPSKKDLKTALDVFAVFQWSFSALLITTTVIAVNLYLVVFTPYWPVTVLILTWLAFDWKTPQRGGRRFTCVRHWRLWKHYSDYFPLKLLKTHDICPSRNYILVCHPHGLFAHGWFGHFATEASGFSKIFPGITPYILTLGAFFWMPFLREYVMSTGACSVSRSSIDFLLTHKGTGNMVIVVIGGLAECRYSLPGSSTLVLKNRSGFVRMALQHGVPLIPAYAFGETDLYDQHIFTPGGFVNRFQKWFQSMVHIYPCAFYGRGFTKNSWGLLPYSRPVTTIVGEPLPMPKIENPSQEIVAKYHTLYIDALRKLFDQHKTKFGISETQELEII

Target ORF information:

RefSeq Version	XM_011530876.2
Organism	Homo sapiens(human)
Definition	Homo sapiens acyl-CoA wax alcohol acyltransferase 2 (AWAT2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011530876.2

ORF Insert Sequence:

ATGCTCTTGC CCTCTAAGAA GGACCTCAAG ACTGCCCTGG ATGTCTTTGC TGTTTTCCAG 
TGGTCCTTCA GTGCCTTGCT TATCACAACC ACTGTGATTG CTGTCAACCT CTACCTGGTG 
GTGTTCACAC CATACTGGCC TGTCACTGTG CTTATTCTTA CCTGGCTGGC TTTTGACTGG 
AAGACCCCTC AGCGAGGCGG CCGCCGGTTT ACCTGTGTGA GGCACTGGCG CCTGTGGAAA 
CACTACAGCG ATTATTTCCC TCTCAAGCTT CTGAAGACTC ATGACATCTG CCCCAGCCGC 
AACTACATCC TCGTCTGCCA CCCTCATGGG CTCTTTGCCC ATGGATGGTT TGGCCACTTT 
GCCACAGAGG CCTCAGGCTT CTCCAAGATA TTTCCTGGCA TCACCCCTTA CATACTCACA 
CTGGGAGCCT TTTTCTGGAT GCCTTTCCTC AGAGAATATG TAATGTCTAC AGGGGCCTGC 
TCTGTGAGTC GATCCTCCAT TGACTTTCTG CTGACTCATA AAGGCACAGG CAACATGGTC 
ATTGTGGTGA TTGGTGGACT GGCTGAGTGC AGATACAGCC TGCCAGGTTC TTCTACCCTG 
GTGTTGAAGA ACCGGTCTGG CTTTGTGCGC ATGGCCCTTC AGCATGGGGT GCCTCTAATA 
CCTGCCTATG CCTTTGGGGA GACGGACCTC TATGATCAGC ACATTTTCAC TCCTGGTGGC 
TTTGTCAACC GCTTCCAGAA GTGGTTCCAG AGCATGGTAC ACATCTACCC TTGTGCTTTC 
TATGGACGTG GCTTCACCAA GAACTCCTGG GGCCTTCTGC CCTATAGTCG GCCTGTAACC 
ACCATCGTCG GGGAGCCTCT ACCAATGCCC AAGATTGAGA ATCCAAGCCA GGAGATCGTG 
GCTAAATATC ACACACTCTA TATTGATGCC CTACGTAAAC TGTTTGACCA GCATAAGACC 
AAGTTTGGTA TCTCAGAGAC CCAGGAGCTG GAGATAATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AWAT2 ( XM_011530876.1 ) cDNA ORF clone, Homo sapiens(Human) -> XP_011529178.1 Homo sapiens acyl-CoA wax alcohol acyltransferase 2 (AWAT2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu28300
Clone ID Related Accession (Same CDS sequence)	XM_011530876.2 , NM_001002254.1 , XM_011530876.1
Accession Version	XM_011530876.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	acyl-CoA wax alcohol acyltransferase 2 isoform X1
Comment

ATGCTCTTGC CCTCTAAGAA GGACCTCAAG ACTGCCCTGG ATGTCTTTGC TGTTTTCCAG 
TGGTCCTTCA GTGCCTTGCT TATCACAACC ACTGTGATTG CTGTCAACCT CTACCTGGTG 
GTGTTCACAC CATACTGGCC TGTCACTGTG CTTATTCTTA CCTGGCTGGC TTTTGACTGG 
AAGACCCCTC AGCGAGGCGG CCGCCGGTTT ACCTGTGTGA GGCACTGGCG CCTGTGGAAA 
CACTACAGCG ATTATTTCCC TCTCAAGCTT CTGAAGACTC ATGACATCTG CCCCAGCCGC 
AACTACATCC TCGTCTGCCA CCCTCATGGG CTCTTTGCCC ATGGATGGTT TGGCCACTTT 
GCCACAGAGG CCTCAGGCTT CTCCAAGATA TTTCCTGGCA TCACCCCTTA CATACTCACA 
CTGGGAGCCT TTTTCTGGAT GCCTTTCCTC AGAGAATATG TAATGTCTAC AGGGGCCTGC 
TCTGTGAGTC GATCCTCCAT TGACTTTCTG CTGACTCATA AAGGCACAGG CAACATGGTC 
ATTGTGGTGA TTGGTGGACT GGCTGAGTGC AGATACAGCC TGCCAGGTTC TTCTACCCTG 
GTGTTGAAGA ACCGGTCTGG CTTTGTGCGC ATGGCCCTTC AGCATGGGGT GCCTCTAATA 
CCTGCCTATG CCTTTGGGGA GACGGACCTC TATGATCAGC ACATTTTCAC TCCTGGTGGC 
TTTGTCAACC GCTTCCAGAA GTGGTTCCAG AGCATGGTAC ACATCTACCC TTGTGCTTTC 
TATGGACGTG GCTTCACCAA GAACTCCTGG GGCCTTCTGC CCTATAGTCG GCCTGTAACC 
ACCATCGTCG GGGAGCCTCT ACCAATGCCC AAGATTGAGA ATCCAAGCCA GGAGATCGTG 
GCTAAATATC ACACACTCTA TATTGATGCC CTACGTAAAC TGTTTGACCA GCATAAGACC 
AAGTTTGGTA TCTCAGAGAC CCAGGAGCTG GAGATAATTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011529178.1
Translation	MLLPSKKDLKTALDVFAVFQWSFSALLITTTVIAVNLYLVVFTPYWPVTVLILTWLAFDWKTPQRGGRRFTCVRHWRLWKHYSDYFPLKLLKTHDICPSRNYILVCHPHGLFAHGWFGHFATEASGFSKIFPGITPYILTLGAFFWMPFLREYVMSTGACSVSRSSIDFLLTHKGTGNMVIVVIGGLAECRYSLPGSSTLVLKNRSGFVRMALQHGVPLIPAYAFGETDLYDQHIFTPGGFVNRFQKWFQSMVHIYPCAFYGRGFTKNSWGLLPYSRPVTTIVGEPLPMPKIENPSQEIVAKYHTLYIDALRKLFDQHKTKFGISETQELEII

Target ORF information:

RefSeq Version	XM_011530876.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens acyl-CoA wax alcohol acyltransferase 2 (AWAT2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011530876.1

ORF Insert Sequence:

ATGCTCTTGC CCTCTAAGAA GGACCTCAAG ACTGCCCTGG ATGTCTTTGC TGTTTTCCAG 
TGGTCCTTCA GTGCCTTGCT TATCACAACC ACTGTGATTG CTGTCAACCT CTACCTGGTG 
GTGTTCACAC CATACTGGCC TGTCACTGTG CTTATTCTTA CCTGGCTGGC TTTTGACTGG 
AAGACCCCTC AGCGAGGCGG CCGCCGGTTT ACCTGTGTGA GGCACTGGCG CCTGTGGAAA 
CACTACAGCG ATTATTTCCC TCTCAAGCTT CTGAAGACTC ATGACATCTG CCCCAGCCGC 
AACTACATCC TCGTCTGCCA CCCTCATGGG CTCTTTGCCC ATGGATGGTT TGGCCACTTT 
GCCACAGAGG CCTCAGGCTT CTCCAAGATA TTTCCTGGCA TCACCCCTTA CATACTCACA 
CTGGGAGCCT TTTTCTGGAT GCCTTTCCTC AGAGAATATG TAATGTCTAC AGGGGCCTGC 
TCTGTGAGTC GATCCTCCAT TGACTTTCTG CTGACTCATA AAGGCACAGG CAACATGGTC 
ATTGTGGTGA TTGGTGGACT GGCTGAGTGC AGATACAGCC TGCCAGGTTC TTCTACCCTG 
GTGTTGAAGA ACCGGTCTGG CTTTGTGCGC ATGGCCCTTC AGCATGGGGT GCCTCTAATA 
CCTGCCTATG CCTTTGGGGA GACGGACCTC TATGATCAGC ACATTTTCAC TCCTGGTGGC 
TTTGTCAACC GCTTCCAGAA GTGGTTCCAG AGCATGGTAC ACATCTACCC TTGTGCTTTC 
TATGGACGTG GCTTCACCAA GAACTCCTGG GGCCTTCTGC CCTATAGTCG GCCTGTAACC 
ACCATCGTCG GGGAGCCTCT ACCAATGCCC AAGATTGAGA ATCCAAGCCA GGAGATCGTG 
GCTAAATATC ACACACTCTA TATTGATGCC CTACGTAAAC TGTTTGACCA GCATAAGACC 
AAGTTTGGTA TCTCAGAGAC CCAGGAGCTG GAGATAATTT GA