CTBS cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CTBS
Entrez Gene ID	1486
Full Name	chitobiase
Synonyms	CTB
General protein information	Preferred Names chitobiase Names di-N-acetylchitobiase chitobiase, di-N-acetyl-
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p22.3
Summary	Chitobiase is a lysosomal glycosidase involved in degradation of asparagine-linked oligosaccharides on glycoproteins (Aronson and Kuranda, 1989 [PubMed 2531691]).[supplied by OMIM, Nov 2010].
Disorder MIM:	600873

mRNA and Protein(s)

mRNA	Protein	Name
NM_004388.3	NP_004379.1	di-N-acetylchitobiase precursor
NM_004388.2	NP_004379.1	di-N-acetylchitobiase precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CTBS	NP_001193529.1
Xenopus tropicalis (tropical clawed frog)	ctbs	NP_001011500.1
Mus musculus (house mouse)	Ctbs	NP_083112.1
Rattus norvegicus (Norway rat)	Ctbs	NP_112285.1
Gallus gallus (chicken)	CTBS	XP_422372.1
Pan troglodytes (chimpanzee)	CTBS	XP_513520.1
Homo sapiens (human)	CTBS	NP_004379.1
Canis lupus familiaris (dog)	CTBS	XP_547309.2
	CTBS	XP_001107057.1
Danio rerio (zebrafish)	ctbs	NP_001018565.1

Gene Ontology
Bibliography

Related articles in PubMed

Fibroblast-Like Synovial Cells in Rheumatoid Arthritis--the Impact of Infliximab on Hexosaminidase Activity.
Olszewski S, Olszewska E, Popko J, Poskrobko E, Sierakowski S, Zwierz K
Advances in clinical and experimental medicine : official organ Wroclaw Medical University24(5)807-13(2015 Sep-Oct)

Optimum substrate size and specific anomer requirements for the reducing-end glycoside hydrolase di-N-acetylchitobiase.
Aronson NN, Halloran BA
Bioscience, biotechnology, and biochemistry70(6)1537-41(2006 Jun)

Human colostrum: identification of minor proteins in the aqueous phase by proteomics.
Palmer DJ, Kelly VC, Smit AM, Kuy S, Knight CG, Cooper GJ
Proteomics6(7)2208-16(2006 Apr)

N-acetyl beta-D-glucosaminidase is not attached to human sperm membranes through the glycosylphosphatidyl inositol (GPI)-anchor.
Hutchinson T, Dwivedi K, Rastogi A, Prasad R, Pereira BM
Asian journal of andrology4(1)27-33(2002 Mar)

Structure of the human gene for lysosomal di-N-acetylchitobiase.
Liu B, Ahmad W, Aronson NN Jr
Glycobiology9(6)589-93(1999 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that infliximab changes the concentration of hexosaminidase (N-acetyl-beta-glucosaminidase; HEX) activity depending on the drug dose and time of administration.
Title: Fibroblast-Like Synovial Cells in Rheumatoid Arthritis--the Impact of Infliximab on Hexosaminidase Activity.

biochemical behavior of di-N-acetylchitobiase indicates it has three subsites, -2, -1, +1, in which the reducing-end trimer of any sized chitooligosaccharide is bound. The +1 site is specific for an alpha-anomer.
Title: Optimum substrate size and specific anomer requirements for the reducing-end glycoside hydrolase di-N-acetylchitobiase.

The following CTBS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CTBS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31463	NM_004388.3 Latest version!	Homo sapiens chitobiase (CTBS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
View Old Accession Versions >>
OHu31463	NM_004388.2	Homo sapiens chitobiase (CTBS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CTBS ( NM_004388.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004379.1 Homo sapiens chitobiase (CTBS), mRNA.

Price & Availability↑

CloneID	OHu31463
Clone ID Related Accession (Same CDS sequence)	NM_004388.3 , NM_004388.2
Accession Version	NM_004388.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Homo sapiens(human)
Product	di-N-acetylchitobiase precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC126335.1, BC065191.1, AI339601.1 and AL359762.24. On Nov 23, 2018 this sequence version replaced NM_004388.2. ##Evidence-Data-START## Transcript exon combination :: AK298703.1, SRR1660809.252331.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000370630.6/ ENSP00000359664.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCCCGGC CGCAGCTTCG ACGCTGGCGC CTCGTCTCTA GCCCGCCGAG CGGCGTCCCG 
GGTCTAGCGC TGCTGGCGCT GCTGGCGCTG CTGGCGCTGC GGCTCGCGGC CGGGACCGAC 
TGCCCATGCC CGGAGCCTGA GCTCTGCCGC CCGATTCGCC ACCATCCAGA TTTCGAGGTC 
TTTGTGTTTG ATGTTGGACA GAAAACTTGG AAATCTTATG ATTGGTCACA GATTACAACT 
GTGGCAACAT TTGGAAAATA TGACTCAGAA CTTATGTGCT ACGCTCATTC AAAAGGAGCC 
AGAGTAGTAC TTAAAGGAGA TGTATCCTTA AAGGATATCA TTGATCCTGC TTTCAGAGCA 
TCCTGGATAG CTCAAAAACT TAATTTGGCC AAAACACAAT ATATGGATGG AATTAATATA 
GATATAGAGC AAGAAGTTAA TTGTTTATCA CCTGAATATG ATGCATTAAC TGCTTTAGTC 
AAAGAAACTA CAGACTCTTT CCATCGTGAA ATTGAGGGAT CACAGGTAAC CTTTGATGTA 
GCTTGGTCTC CAAAGAACAT AGACAGAAGA TGCTATAATT ATACTGGAAT CGCAGATGCT 
TGTGACTTCC TCTTTGTGAT GTCTTATGAT GAACAAAGTC AGATCTGGTC AGAATGTATT 
GCAGCAGCCA ATGCTCCCTA TAATCAGACA TTAACTGGAT ATAATGACTA CATCAAGATG 
AGCATTAATC CTAAGAAACT TGTAATGGGT GTTCCTTGGT ATGGTTATGA TTATACCTGC 
CTGAATCTGT CTGAGGATCA TGTTTGTACC ATTGCAAAAG TCCCTTTCCG GGGGGCTCCT 
TGTAGTGACG CTGCAGGACG TCAGGTGCCC TACAAAACGA TCATGAAGCA AATAAATAGT 
TCTATTTCTG GAAACCTATG GGATAAAGAT CAGCGGGCTC CTTATTATAA CTATAAAGAT 
CCTGCTGGCC ACTTTCATCA AGTATGGTAT GATAACCCTC AGAGTATTTC TTTAAAGGCA 
ACATATATAC AAAACTATCG CTTACGGGGC ATTGGCATGT GGAATGCAAA CTGTCTTGAC 
TACTCTGGAG ATGCTGTAGC CAAACAGCAA ACTGAAGAAA TGTGGGAAGT CTTAAAGCCA 
AAGCTGTTAC AGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004379.1
CDS	26..1183
Translation	MSRPQLRRWRLVSSPPSGVPGLALLALLALLALRLAAGTDCPCPEPELCRPIRHHPDFEVFVFDVGQKTWKSYDWSQITTVATFGKYDSELMCYAHSKGARVVLKGDVSLKDIIDPAFRASWIAQKLNLAKTQYMDGINIDIEQEVNCLSPEYDALTALVKETTDSFHREIEGSQVTFDVAWSPKNIDRRCYNYTGIADACDFLFVMSYDEQSQIWSECIAAANAPYNQTLTGYNDYIKMSINPKKLVMGVPWYGYDYTCLNLSEDHVCTIAKVPFRGAPCSDAAGRQVPYKTIMKQINSSISGNLWDKDQRAPYYNYKDPAGHFHQVWYDNPQSISLKATYIQNYRLRGIGMWNANCLDYSGDAVAKQQTEEMWEVLKPKLLQR

Target ORF information:

RefSeq Version	NM_004388.3
Organism	Homo sapiens(human)
Definition	Homo sapiens chitobiase (CTBS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004388.3

ORF Insert Sequence:

ATGTCCCGGC CGCAGCTTCG ACGCTGGCGC CTCGTCTCTA GCCCGCCGAG CGGCGTCCCG 
GGTCTAGCGC TGCTGGCGCT GCTGGCGCTG CTGGCGCTGC GGCTCGCGGC CGGGACCGAC 
TGCCCATGCC CGGAGCCTGA GCTCTGCCGC CCGATTCGCC ACCATCCAGA TTTCGAGGTC 
TTTGTGTTTG ATGTTGGACA GAAAACTTGG AAATCTTATG ATTGGTCACA GATTACAACT 
GTGGCAACAT TTGGAAAATA TGACTCAGAA CTTATGTGCT ACGCTCATTC AAAAGGAGCC 
AGAGTAGTAC TTAAAGGAGA TGTATCCTTA AAGGATATCA TTGATCCTGC TTTCAGAGCA 
TCCTGGATAG CTCAAAAACT TAATTTGGCC AAAACACAAT ATATGGATGG AATTAATATA 
GATATAGAGC AAGAAGTTAA TTGTTTATCA CCTGAATATG ATGCATTAAC TGCTTTAGTC 
AAAGAAACTA CAGACTCTTT CCATCGTGAA ATTGAGGGAT CACAGGTAAC CTTTGATGTA 
GCTTGGTCTC CAAAGAACAT AGACAGAAGA TGCTATAATT ATACTGGAAT CGCAGATGCT 
TGTGACTTCC TCTTTGTGAT GTCTTATGAT GAACAAAGTC AGATCTGGTC AGAATGTATT 
GCAGCAGCCA ATGCTCCCTA TAATCAGACA TTAACTGGAT ATAATGACTA CATCAAGATG 
AGCATTAATC CTAAGAAACT TGTAATGGGT GTTCCTTGGT ATGGTTATGA TTATACCTGC 
CTGAATCTGT CTGAGGATCA TGTTTGTACC ATTGCAAAAG TCCCTTTCCG GGGGGCTCCT 
TGTAGTGACG CTGCAGGACG TCAGGTGCCC TACAAAACGA TCATGAAGCA AATAAATAGT 
TCTATTTCTG GAAACCTATG GGATAAAGAT CAGCGGGCTC CTTATTATAA CTATAAAGAT 
CCTGCTGGCC ACTTTCATCA AGTATGGTAT GATAACCCTC AGAGTATTTC TTTAAAGGCA 
ACATATATAC AAAACTATCG CTTACGGGGC ATTGGCATGT GGAATGCAAA CTGTCTTGAC 
TACTCTGGAG ATGCTGTAGC CAAACAGCAA ACTGAAGAAA TGTGGGAAGT CTTAAAGCCA 
AAGCTGTTAC AGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CTBS ( NM_004388.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_004379.1 Homo sapiens chitobiase (CTBS), mRNA.

Price & Availability↑

CloneID	OHu31463
Clone ID Related Accession (Same CDS sequence)	NM_004388.3 , NM_004388.2
Accession Version	NM_004388.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	di-N-acetylchitobiase precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC024007.2, BC126335.1, BC065191.1 and AI339601.1. On Sep 4, 2008 this sequence version replaced NM_004388.1. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.252331.1, SRR1660809.117336.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGTCCCGGC CGCAGCTTCG ACGCTGGCGC CTCGTCTCTA GCCCGCCGAG CGGCGTCCCG 
GGTCTAGCGC TGCTGGCGCT GCTGGCGCTG CTGGCGCTGC GGCTCGCGGC CGGGACCGAC 
TGCCCATGCC CGGAGCCTGA GCTCTGCCGC CCGATTCGCC ACCATCCAGA TTTCGAGGTC 
TTTGTGTTTG ATGTTGGACA GAAAACTTGG AAATCTTATG ATTGGTCACA GATTACAACT 
GTGGCAACAT TTGGAAAATA TGACTCAGAA CTTATGTGCT ACGCTCATTC AAAAGGAGCC 
AGAGTAGTAC TTAAAGGAGA TGTATCCTTA AAGGATATCA TTGATCCTGC TTTCAGAGCA 
TCCTGGATAG CTCAAAAACT TAATTTGGCC AAAACACAAT ATATGGATGG AATTAATATA 
GATATAGAGC AAGAAGTTAA TTGTTTATCA CCTGAATATG ATGCATTAAC TGCTTTAGTC 
AAAGAAACTA CAGACTCTTT CCATCGTGAA ATTGAGGGAT CACAGGTAAC CTTTGATGTA 
GCTTGGTCTC CAAAGAACAT AGACAGAAGA TGCTATAATT ATACTGGAAT CGCAGATGCT 
TGTGACTTCC TCTTTGTGAT GTCTTATGAT GAACAAAGTC AGATCTGGTC AGAATGTATT 
GCAGCAGCCA ATGCTCCCTA TAATCAGACA TTAACTGGAT ATAATGACTA CATCAAGATG 
AGCATTAATC CTAAGAAACT TGTAATGGGT GTTCCTTGGT ATGGTTATGA TTATACCTGC 
CTGAATCTGT CTGAGGATCA TGTTTGTACC ATTGCAAAAG TCCCTTTCCG GGGGGCTCCT 
TGTAGTGACG CTGCAGGACG TCAGGTGCCC TACAAAACGA TCATGAAGCA AATAAATAGT 
TCTATTTCTG GAAACCTATG GGATAAAGAT CAGCGGGCTC CTTATTATAA CTATAAAGAT 
CCTGCTGGCC ACTTTCATCA AGTATGGTAT GATAACCCTC AGAGTATTTC TTTAAAGGCA 
ACATATATAC AAAACTATCG CTTACGGGGC ATTGGCATGT GGAATGCAAA CTGTCTTGAC 
TACTCTGGAG ATGCTGTAGC CAAACAGCAA ACTGAAGAAA TGTGGGAAGT CTTAAAGCCA 
AAGCTGTTAC AGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004379.1
CDS	66..1223
Translation	MSRPQLRRWRLVSSPPSGVPGLALLALLALLALRLAAGTDCPCPEPELCRPIRHHPDFEVFVFDVGQKTWKSYDWSQITTVATFGKYDSELMCYAHSKGARVVLKGDVSLKDIIDPAFRASWIAQKLNLAKTQYMDGINIDIEQEVNCLSPEYDALTALVKETTDSFHREIEGSQVTFDVAWSPKNIDRRCYNYTGIADACDFLFVMSYDEQSQIWSECIAAANAPYNQTLTGYNDYIKMSINPKKLVMGVPWYGYDYTCLNLSEDHVCTIAKVPFRGAPCSDAAGRQVPYKTIMKQINSSISGNLWDKDQRAPYYNYKDPAGHFHQVWYDNPQSISLKATYIQNYRLRGIGMWNANCLDYSGDAVAKQQTEEMWEVLKPKLLQR

Target ORF information:

RefSeq Version	NM_004388.2
Organism	Homo sapiens(human)
Definition	Homo sapiens chitobiase (CTBS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004388.2

ORF Insert Sequence:

ATGTCCCGGC CGCAGCTTCG ACGCTGGCGC CTCGTCTCTA GCCCGCCGAG CGGCGTCCCG 
GGTCTAGCGC TGCTGGCGCT GCTGGCGCTG CTGGCGCTGC GGCTCGCGGC CGGGACCGAC 
TGCCCATGCC CGGAGCCTGA GCTCTGCCGC CCGATTCGCC ACCATCCAGA TTTCGAGGTC 
TTTGTGTTTG ATGTTGGACA GAAAACTTGG AAATCTTATG ATTGGTCACA GATTACAACT 
GTGGCAACAT TTGGAAAATA TGACTCAGAA CTTATGTGCT ACGCTCATTC AAAAGGAGCC 
AGAGTAGTAC TTAAAGGAGA TGTATCCTTA AAGGATATCA TTGATCCTGC TTTCAGAGCA 
TCCTGGATAG CTCAAAAACT TAATTTGGCC AAAACACAAT ATATGGATGG AATTAATATA 
GATATAGAGC AAGAAGTTAA TTGTTTATCA CCTGAATATG ATGCATTAAC TGCTTTAGTC 
AAAGAAACTA CAGACTCTTT CCATCGTGAA ATTGAGGGAT CACAGGTAAC CTTTGATGTA 
GCTTGGTCTC CAAAGAACAT AGACAGAAGA TGCTATAATT ATACTGGAAT CGCAGATGCT 
TGTGACTTCC TCTTTGTGAT GTCTTATGAT GAACAAAGTC AGATCTGGTC AGAATGTATT 
GCAGCAGCCA ATGCTCCCTA TAATCAGACA TTAACTGGAT ATAATGACTA CATCAAGATG 
AGCATTAATC CTAAGAAACT TGTAATGGGT GTTCCTTGGT ATGGTTATGA TTATACCTGC 
CTGAATCTGT CTGAGGATCA TGTTTGTACC ATTGCAAAAG TCCCTTTCCG GGGGGCTCCT 
TGTAGTGACG CTGCAGGACG TCAGGTGCCC TACAAAACGA TCATGAAGCA AATAAATAGT 
TCTATTTCTG GAAACCTATG GGATAAAGAT CAGCGGGCTC CTTATTATAA CTATAAAGAT 
CCTGCTGGCC ACTTTCATCA AGTATGGTAT GATAACCCTC AGAGTATTTC TTTAAAGGCA 
ACATATATAC AAAACTATCG CTTACGGGGC ATTGGCATGT GGAATGCAAA CTGTCTTGAC 
TACTCTGGAG ATGCTGTAGC CAAACAGCAA ACTGAAGAAA TGTGGGAAGT CTTAAAGCCA 
AAGCTGTTAC AGAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Fibroblast-Like Synovial Cells in Rheumatoid Arthritis--the Impact of Infliximab on Hexosaminidase Activity.
Advances in clinical and experimental medicine : official organ Wroclaw Medical University24(5)807-13(2015 Sep-Oct)
Olszewski S,Olszewska E,Popko J,Poskrobko E,Sierakowski S,Zwierz K

Optimum substrate size and specific anomer requirements for the reducing-end glycoside hydrolase di-N-acetylchitobiase.
Bioscience, biotechnology, and biochemistry70(6)1537-41(2006 Jun)
Aronson NN,Halloran BA

Human colostrum: identification of minor proteins in the aqueous phase by proteomics.
Proteomics6(7)2208-16(2006 Apr)
Palmer DJ,Kelly VC,Smit AM,Kuy S,Knight CG,Cooper GJ

N-acetyl beta-D-glucosaminidase is not attached to human sperm membranes through the glycosylphosphatidyl inositol (GPI)-anchor.
Asian journal of andrology4(1)27-33(2002 Mar)
Hutchinson T,Dwivedi K,Rastogi A,Prasad R,Pereira BM

Structure of the human gene for lysosomal di-N-acetylchitobiase.
Glycobiology9(6)589-93(1999 Jun)
Liu B,Ahmad W,Aronson NN Jr