DGKK cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DGKK
Entrez Gene ID	139189
Full Name	diacylglycerol kinase kappa
General protein information	Preferred Names diacylglycerol kinase kappa Names diacylglycerol kinase kappa 142 kDa diacylglycerol kinase DAG kinase kappa DGK-kappa diglyceride kinase kappa
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xp11.22
Summary	The protein encoded by this gene is an enzyme that phosphorylates diacylglycerol, converting it to phosphatidic acid. The encoded protein is a membrane protein and is inhibited by hydrogen peroxide. Variations in this gene have been associated with hypospadias. [provided by RefSeq, Mar 2011].
Disorder MIM:	300837

mRNA and Protein(s)

mRNA	Protein	Name
NM_001013742.3	NP_001013764.1	diacylglycerol kinase kappa
XM_017029268.1	XP_016884757.1	diacylglycerol kinase kappa isoform X1
NM_001013742.4	NP_001013764.1	diacylglycerol kinase kappa
XM_017029268.2	XP_016884757.1	diacylglycerol kinase kappa isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DGKK	XP_528979.2
Canis lupus familiaris (dog)	DGKK	XP_549004.3
Homo sapiens (human)	DGKK	NP_001013764.1
	DGKK	XP_001083870.1
Bos taurus (cattle)	DGKK	XP_002700155.2
Rattus norvegicus (Norway rat)	Dgkk	XP_346277.2
Mus musculus (house mouse)	Dgkk	NP_808582.3

Gene Ontology
Bibliography

Related articles in PubMed

Association between diacylglycerol kinase kappa variants and hypospadias susceptibility in a Han Chinese population.
Xie H, Lin XL, Zhang S, Yu L, Li XX, Huang YC, Lyu YQ, Chen HT, Xu J, Chen F
Asian journal of andrology20(1)85-89(2018 Jan-Feb)

Fragile X Mental Retardation Protein (FMRP) controls diacylglycerol kinase activity in neurons.
Tabet R, Moutin E, Becker JA, Heintz D, Fouillen L, Flatter E, Kr??el W, Alunni V, Koebel P, Demb?l? D, Tassone F, Bardoni B, Mandel JL, Vitale N, Muller D, Le Merrer J, Moine H
Proceedings of the National Academy of Sciences of the United States of America113(26)E3619-28(2016 06)

Expression Analysis of DGKK during External Genitalia Formation.
Shen J, Liu B, Sinclair A, Cunha G, Baskin LS, Choudhry S
The Journal of urology194(6)1728-36(2015 Dec)

Diacylglycerol kinase ? (DGKK) variants and hypospadias in Han Chinese: association and meta-analysis.
Ma Q, Tang Y, Lin H, Xu M, Xu G, Fang X, Chen J, Song Z, Li Z, Shi Y, Geng H
BJU international116(4)634-40(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

FMRP is mostly associated diacylglycerol kinase kappa (Dgkkappa), a master regulator that controls the switch between diacylglycerol and phosphatidic acid signaling pathways.
Title: Fragile X Mental Retardation Protein (FMRP) controls diacylglycerol kinase activity in neurons.

Dgkk appears to be a marker or mediator of squamous cell differentiation in development of mouse external genitalia. No association exists between Dgkk expression and formation of preputial cleft in the genital tubercle of diethylsilbestrol treated mice.
Title: Expression Analysis of DGKK during External Genitalia Formation.

Studied the relationship between DGKK single nucleotide polymorphisms (SNPs) and hypospadias risk in the Han Chinese population.
Title: Diacylglycerol kinase ? (DGKK) variants and hypospadias in Han Chinese: association and meta-analysis.

Common variants in DGKK are strongly associated with risk of hypospadias
Title: Common variants in DGKK are strongly associated with risk of hypospadias.

DGK kappa is a novel human type II diacylglycerol kinase
Title: Identification and characterization of a novel human type II diacylglycerol kinase, DGK kappa.

The following DGKK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DGKK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu21039	NM_001013742.4 Latest version!	Homo sapiens diacylglycerol kinase kappa (DGKK), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu99152	XM_017029268.2 Latest version!	Homo sapiens diacylglycerol kinase kappa (DGKK), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
View Old Accession Versions >>
OHu21039	NM_001013742.3	Homo sapiens diacylglycerol kinase kappa (DGKK), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu99152	XM_017029268.1	Homo sapiens diacylglycerol kinase kappa (DGKK), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DGKK ( NM_001013742.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001013764.1 Homo sapiens diacylglycerol kinase kappa (DGKK), mRNA.

Price & Availability↑

CloneID	OHu21039
Clone ID Related Accession (Same CDS sequence)	NM_001013742.3 , NM_001013742.4
Accession Version	NM_001013742.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Homo sapiens(human)
Product	diacylglycerol kinase kappa
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB183864.1, AC239396.3 and AI591038.1. This sequence is a reference standard in the RefSeqGene project. On Feb 11, 2014 this sequence version replaced NM_001013742.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AB183864.1, BC137319.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145544, SAMEA2162841 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCGCG GAGCTGCCGC AGCCCAGGGC ACTGCCCCGC CTCAGGATGG AGAGCAGCCC 
GCTGAGTCTC CAGAGCCTCC GCCGCCTTGG CCGCCGCCGC CACCACCACC GGCTCCGCCG 
CCGGCTCCGC CGCTGCTCTC CGAGGCTTCG CCAGAACCCA TACCAGAGCC CTGTCCAGAG 
CTTGCTCCAG GTCCCTGTCC AGAGGCGACC TCAGAATCAG CCACAGAACT GTACACAGAA 
CCGACCCCAG AACCAGCCAC AGAGCCGGCC TCAGAACCGG CCCCAGAACC TGCCACAGAG 
CCGGCCCCAG AACCTGCCAC AGAGCCGGCC CCAGAACCGG CCCCAGAACC TGCCACAGAG 
TCTGCCCCAG AGCCGACTCC AGAACCTGCC CTAGAGTCGG TCCCAGAGCC GGCCCCAGAG 
CTGACTCCAG AAGTTGCCCC AGAGCTGGCC CCAGAGCCGA CCCCAGAACC TGTGACAGAG 
CTGGCCCCAG AGTTCTGCCC TGAGGCGGCT CCAGAGTTCC GTCCAAGTCC AGCACCATGT 
CTATTGCAAT GTCCGGTGGA CACTCGAGAG AGAGGTCTAA AGACCTCGCC ATCGCCATCG 
CCATCGCCAT CGCCCAGAAC GCCAATGTCG TGGTCCAGAA TAAAGAAAAT ATTGAAGGAA 
GGACCTATGC TGAAGAACTG TAACTCTTTC AAGAGATGGA AGCTTAGATA TTTTCTGGTT 
CAAGGACAGA AGCTCTACTT TGCACACCAT CCCGCGTTTG CACACTTTGA AACGATTGAT 
CTGTCTCAAG CCACTGTGGC AGAAAGCAGC TGTAGAAACC TTTGCCACAG TTTTTGTGTT 
ATTACACCAC AACGAAAAAT CACTCTGGCT GCACCCAACC GGAAAGACAT GGAAGAATGG 
ATTAACATCA TAAAAACCAT CCAACAGGGA GAAATTTATA AGATACCTGC AGCAGAAAAC 
AACCCTTTTC TTGTTGGAAT GCATTGTTGG TACTCCAGTT ACAGCCACCG GACCCAGCAC 
TGCAATGTTT GTCGAGAGAG CATTCCTGCC TTATCTAGAG ATGCCATCAT CTGTGAAGTG 
TGCAAAGTGA AATCTCACAG ATTGTGTGCT TTGAGAGCAA GCAAAGACTG CAAGTGGAAT 
ACATTGTCTA TCACTGATGA CCTCCTTCTG CCTGCAGATG AAGTAAACAT GCCCCATCAA 
TGGGTAGAAG GAAACATGCC TGTCAGCTCT CAGTGTGCAG TGTGTCATGA GAGCTGTGGC 
AGTTATCAAA GACTTCAAGA CTTCCGCTGC CTGTGGTGTA ATTCTACGGT GCATGATGAC 
TGTAGGAGAC GGTTTTCCAA GGAATGTTGC TTCAGAAGCC ATCGCTCATC AGTCATTCCT 
CCCACTGCTC TAAGCGACCC CAAAGGCGAT GGCCAATTAG TAGTATCTTC AGACTTCTGG 
AATCTTGATT GGTCATCAGC CTGTTCATGT CCCTTGCTCA TCTTCATCAA CTCCAAAAGT 
GGCGATCATC AGGGGATCGT CTTCCTCCGA AAATTCAAGC AATACCTTAA CCCATCTCAA 
GTGTTCGACT TATTGAAGGG TGGACCTGAA GCAGGGCTGT CTATGTTCAA GAACTTTGCT 
CGCTTTCGCA TTCTGGTTTG TGGTGGAGAT GGCAGCGTGA GCTGGGTCTT ATCTCTGATT 
GATGCCTTTG GATTACATGA AAAGTGTCAG TTGGCAGTCA TCCCACTTGG AACCGGCAAT 
GATCTGGCTC GTGTTCTGGG CTGGGGTGCA TTCTGGAACA AAAGCAAGTC ACCTCTGGAC 
ATCCTCAACA GAGTGGAGCA GGCTAGTGTG AGGATCCTAG ACAGATGGAG TGTGATGATT 
CGTGAGACTC CCAGACAAAC CCCGCTGCTA AAAGGACAGG TTGAAATGGA TGTACCACGA 
TTTGAGGCTG CTGCCATCCA ACACTTAGAA TCTGCAGCCA CCGAGTTGAA CAAAATCCTG 
AAGGCCAAGT ACCCCACAGA GATGATCATC GCAACCAGAT TCTTGTGTTC AGCTGTGGAA 
GATTTTGTGG TTGATATTGT AAAGGCCTGG GGTCAGATAA AACAGAACAA CACTGCAATA 
GTGTCTGTGA TTTTGAAAAG TGACTTAATG TATGATAGGC TCAGTGTCCT GATCGATGTC 
CTGGCTGAGG AGGCAGCAGC TACTTCTGCT GAAAAAAGTG CTACAGAATA TGCAGACAGC 
AGCAAGGCAG ATAGGAAGCC CTTCATTCCT CAAATAGACC ACATAGCCAA GTGCAAGTTG 
GAGCTGGCTA CAAAGGCCCA GAGTCTCCAG AAATCCTTGA AACTCATCAT ATTTCAAGTT 
GAACAAGCTC TGGATGAGGA AAGCAGACAG ACAATATCTG TTAAGAACTT TAGTTCAACT 
TTCTTCCTGG AAGATGACCC AGAAGATATT AACCAGACAA GCCCACGACG CCGTTCTCGT 
CGTGGCACTT TGTCTTCTAT ATCTTCTCTC AAAAGTGAGG ACCTGGACAA CCTTAACTTG 
GATCACTTAC ATTTTACACC TGAATCTATA CGCTTCAAAG AAAAATGTGT CATGAACAAC 
TACTTCGGAA TTGGACTGGA TGCTAAAATT TCTCTGGACT TCAACACCAG AAGAGATGAA 
CACCCAGGGC AATACAATAG CCGCCTTAAG AACAAGATGT GGTATGGCCT TCTGGGAACC 
AAAGAACTTT TGCAGCGCTC TTACAGGAAA CTGGAAGAAC GAGTGCATTT GGAGTGTGAT 
GGAGAAACCA TCTCCTTGCC AAACCTGCAA GGCATTGTAG TGCTCAACAT TACCAGCTAT 
GCTGGAGGTA TCAACTTCTG GGGAAGCAAC ACAGCAACCA CGGAATATGA GGCTCCTGCA 
ATCGATGATG GGAAACTGGA GGTGGTGGCA ATCTTTGGTT CTGTGCAGAT GGCAATGTCC 
CGTATCATCA ACCTGCATCA TCATCGCATT GCCCAGTGCC ATGAGGTGAT GATAACCATT 
GATGGTGAAG AAGGTATCCC AGTGCAGGTG GATGGGGAGG CCTGGATTCA GAGACCAGGC 
CTTATCAAAA TTAGATACAA GAACGCTGCC CAGATGCTGA CAAGAGATCG GGACTTTGAG 
AACTCAATGA AAATGTGGGA ATACAAGCAT ACTGAAATTC AAGCTGCCCC TCAACCCCAG 
CTGGACTTCC AGGACTCTCA AGAGAGCCTC TCTGACGAGG AGTATGCCCA GATGCAGCAC 
TTAGCTCGGC TTGCAGAAAA CCTCATCAGC AAACTTAATG ACCTGAGCAA GATCCACCAG 
CATGTGTCTG TCCTCATGGG TTCTGTGAAT GCCAGCGCTA ACATCCTGAA TGATATATTT 
TACGGCCAAG ACAGTGGCAA TGAGATGGGT GCAGCTTCCT GTATTCCCAT TGAAACTCTA 
AGCAGAAATG ATGCCGTAGA TGTTACATTT AGTCTTAAAG GTCTCTACGA TGACACCACA 
GCTTTCCTGG ATGAAAAGCT GCTGAGAAGT GCTGAGGATG AGACTGCACT ACAAAGCGCC 
CTGGATGCCA TGAATAAGGA GTTCAAAAAG CTATCTGAGA TTGACTGGAT GAATCCAATC 
TTTGTTCCAG AGGAAAAATC TTCGGACACT GACAGTAGAA GCCTCAGGCT GAAAATTAAG 
TTCCCCAAAT TGGGAAAGAA AAAGGTAGAA GAGGAACGCA AGCCTAAATC AGGCCAGAGT 
GTCCAGAGTT TTATTGGCAA TTTATGGCAC CGCAGACATC GTGAAGATGA AGCAGAGGGT 
GATGATCCTC TAACACCATC GAGATCTCAA CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001013764.1
CDS	61..3876
Translation	MDRGAAAAQGTAPPQDGEQPAESPEPPPPWPPPPPPPAPPPAPPLLSEASPEPIPEPCPELAPGPCPEATSESATELYTEPTPEPATEPASEPAPEPATEPAPEPATEPAPEPAPEPATESAPEPTPEPALESVPEPAPELTPEVAPELAPEPTPEPVTELAPEFCPEAAPEFRPSPAPCLLQCPVDTRERGLKTSPSPSPSPSPRTPMSWSRIKKILKEGPMLKNCNSFKRWKLRYFLVQGQKLYFAHHPAFAHFETIDLSQATVAESSCRNLCHSFCVITPQRKITLAAPNRKDMEEWINIIKTIQQGEIYKIPAAENNPFLVGMHCWYSSYSHRTQHCNVCRESIPALSRDAIICEVCKVKSHRLCALRASKDCKWNTLSITDDLLLPADEVNMPHQWVEGNMPVSSQCAVCHESCGSYQRLQDFRCLWCNSTVHDDCRRRFSKECCFRSHRSSVIPPTALSDPKGDGQLVVSSDFWNLDWSSACSCPLLIFINSKSGDHQGIVFLRKFKQYLNPSQVFDLLKGGPEAGLSMFKNFARFRILVCGGDGSVSWVLSLIDAFGLHEKCQLAVIPLGTGNDLARVLGWGAFWNKSKSPLDILNRVEQASVRILDRWSVMIRETPRQTPLLKGQVEMDVPRFEAAAIQHLESAATELNKILKAKYPTEMIIATRFLCSAVEDFVVDIVKAWGQIKQNNTAIVSVILKSDLMYDRLSVLIDVLAEEAAATSAEKSATEYADSSKADRKPFIPQIDHIAKCKLELATKAQSLQKSLKLIIFQVEQALDEESRQTISVKNFSSTFFLEDDPEDINQTSPRRRSRRGTLSSISSLKSEDLDNLNLDHLHFTPESIRFKEKCVMNNYFGIGLDAKISLDFNTRRDEHPGQYNSRLKNKMWYGLLGTKELLQRSYRKLEERVHLECDGETISLPNLQGIVVLNITSYAGGINFWGSNTATTEYEAPAIDDGKLEVVAIFGSVQMAMSRIINLHHHRIAQCHEVMITIDGEEGIPVQVDGEAWIQRPGLIKIRYKNAAQMLTRDRDFENSMKMWEYKHTEIQAAPQPQLDFQDSQESLSDEEYAQMQHLARLAENLISKLNDLSKIHQHVSVLMGSVNASANILNDIFYGQDSGNEMGAASCIPIETLSRNDAVDVTFSLKGLYDDTTAFLDEKLLRSAEDETALQSALDAMNKEFKKLSEIDWMNPIFVPEEKSSDTDSRSLRLKIKFPKLGKKKVEEERKPKSGQSVQSFIGNLWHRRHREDEAEGDDPLTPSRSQL

Target ORF information:

RefSeq Version	NM_001013742.3
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase kappa (DGKK), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001013742.3

ORF Insert Sequence:

ATGGACCGCG GAGCTGCCGC AGCCCAGGGC ACTGCCCCGC CTCAGGATGG AGAGCAGCCC 
GCTGAGTCTC CAGAGCCTCC GCCGCCTTGG CCGCCGCCGC CACCACCACC GGCTCCGCCG 
CCGGCTCCGC CGCTGCTCTC CGAGGCTTCG CCAGAACCCA TACCAGAGCC CTGTCCAGAG 
CTTGCTCCAG GTCCCTGTCC AGAGGCGACC TCAGAATCAG CCACAGAACT GTACACAGAA 
CCGACCCCAG AACCAGCCAC AGAGCCGGCC TCAGAACCGG CCCCAGAACC TGCCACAGAG 
CCGGCCCCAG AACCTGCCAC AGAGCCGGCC CCAGAACCGG CCCCAGAACC TGCCACAGAG 
TCTGCCCCAG AGCCGACTCC AGAACCTGCC CTAGAGTCGG TCCCAGAGCC GGCCCCAGAG 
CTGACTCCAG AAGTTGCCCC AGAGCTGGCC CCAGAGCCGA CCCCAGAACC TGTGACAGAG 
CTGGCCCCAG AGTTCTGCCC TGAGGCGGCT CCAGAGTTCC GTCCAAGTCC AGCACCATGT 
CTATTGCAAT GTCCGGTGGA CACTCGAGAG AGAGGTCTAA AGACCTCGCC ATCGCCATCG 
CCATCGCCAT CGCCCAGAAC GCCAATGTCG TGGTCCAGAA TAAAGAAAAT ATTGAAGGAA 
GGACCTATGC TGAAGAACTG TAACTCTTTC AAGAGATGGA AGCTTAGATA TTTTCTGGTT 
CAAGGACAGA AGCTCTACTT TGCACACCAT CCCGCGTTTG CACACTTTGA AACGATTGAT 
CTGTCTCAAG CCACTGTGGC AGAAAGCAGC TGTAGAAACC TTTGCCACAG TTTTTGTGTT 
ATTACACCAC AACGAAAAAT CACTCTGGCT GCACCCAACC GGAAAGACAT GGAAGAATGG 
ATTAACATCA TAAAAACCAT CCAACAGGGA GAAATTTATA AGATACCTGC AGCAGAAAAC 
AACCCTTTTC TTGTTGGAAT GCATTGTTGG TACTCCAGTT ACAGCCACCG GACCCAGCAC 
TGCAATGTTT GTCGAGAGAG CATTCCTGCC TTATCTAGAG ATGCCATCAT CTGTGAAGTG 
TGCAAAGTGA AATCTCACAG ATTGTGTGCT TTGAGAGCAA GCAAAGACTG CAAGTGGAAT 
ACATTGTCTA TCACTGATGA CCTCCTTCTG CCTGCAGATG AAGTAAACAT GCCCCATCAA 
TGGGTAGAAG GAAACATGCC TGTCAGCTCT CAGTGTGCAG TGTGTCATGA GAGCTGTGGC 
AGTTATCAAA GACTTCAAGA CTTCCGCTGC CTGTGGTGTA ATTCTACGGT GCATGATGAC 
TGTAGGAGAC GGTTTTCCAA GGAATGTTGC TTCAGAAGCC ATCGCTCATC AGTCATTCCT 
CCCACTGCTC TAAGCGACCC CAAAGGCGAT GGCCAATTAG TAGTATCTTC AGACTTCTGG 
AATCTTGATT GGTCATCAGC CTGTTCATGT CCCTTGCTCA TCTTCATCAA CTCCAAAAGT 
GGCGATCATC AGGGGATCGT CTTCCTCCGA AAATTCAAGC AATACCTTAA CCCATCTCAA 
GTGTTCGACT TATTGAAGGG TGGACCTGAA GCAGGGCTGT CTATGTTCAA GAACTTTGCT 
CGCTTTCGCA TTCTGGTTTG TGGTGGAGAT GGCAGCGTGA GCTGGGTCTT ATCTCTGATT 
GATGCCTTTG GATTACATGA AAAGTGTCAG TTGGCAGTCA TCCCACTTGG AACCGGCAAT 
GATCTGGCTC GTGTTCTGGG CTGGGGTGCA TTCTGGAACA AAAGCAAGTC ACCTCTGGAC 
ATCCTCAACA GAGTGGAGCA GGCTAGTGTG AGGATCCTAG ACAGATGGAG TGTGATGATT 
CGTGAGACTC CCAGACAAAC CCCGCTGCTA AAAGGACAGG TTGAAATGGA TGTACCACGA 
TTTGAGGCTG CTGCCATCCA ACACTTAGAA TCTGCAGCCA CCGAGTTGAA CAAAATCCTG 
AAGGCCAAGT ACCCCACAGA GATGATCATC GCAACCAGAT TCTTGTGTTC AGCTGTGGAA 
GATTTTGTGG TTGATATTGT AAAGGCCTGG GGTCAGATAA AACAGAACAA CACTGCAATA 
GTGTCTGTGA TTTTGAAAAG TGACTTAATG TATGATAGGC TCAGTGTCCT GATCGATGTC 
CTGGCTGAGG AGGCAGCAGC TACTTCTGCT GAAAAAAGTG CTACAGAATA TGCAGACAGC 
AGCAAGGCAG ATAGGAAGCC CTTCATTCCT CAAATAGACC ACATAGCCAA GTGCAAGTTG 
GAGCTGGCTA CAAAGGCCCA GAGTCTCCAG AAATCCTTGA AACTCATCAT ATTTCAAGTT 
GAACAAGCTC TGGATGAGGA AAGCAGACAG ACAATATCTG TTAAGAACTT TAGTTCAACT 
TTCTTCCTGG AAGATGACCC AGAAGATATT AACCAGACAA GCCCACGACG CCGTTCTCGT 
CGTGGCACTT TGTCTTCTAT ATCTTCTCTC AAAAGTGAGG ACCTGGACAA CCTTAACTTG 
GATCACTTAC ATTTTACACC TGAATCTATA CGCTTCAAAG AAAAATGTGT CATGAACAAC 
TACTTCGGAA TTGGACTGGA TGCTAAAATT TCTCTGGACT TCAACACCAG AAGAGATGAA 
CACCCAGGGC AATACAATAG CCGCCTTAAG AACAAGATGT GGTATGGCCT TCTGGGAACC 
AAAGAACTTT TGCAGCGCTC TTACAGGAAA CTGGAAGAAC GAGTGCATTT GGAGTGTGAT 
GGAGAAACCA TCTCCTTGCC AAACCTGCAA GGCATTGTAG TGCTCAACAT TACCAGCTAT 
GCTGGAGGTA TCAACTTCTG GGGAAGCAAC ACAGCAACCA CGGAATATGA GGCTCCTGCA 
ATCGATGATG GGAAACTGGA GGTGGTGGCA ATCTTTGGTT CTGTGCAGAT GGCAATGTCC 
CGTATCATCA ACCTGCATCA TCATCGCATT GCCCAGTGCC ATGAGGTGAT GATAACCATT 
GATGGTGAAG AAGGTATCCC AGTGCAGGTG GATGGGGAGG CCTGGATTCA GAGACCAGGC 
CTTATCAAAA TTAGATACAA GAACGCTGCC CAGATGCTGA CAAGAGATCG GGACTTTGAG 
AACTCAATGA AAATGTGGGA ATACAAGCAT ACTGAAATTC AAGCTGCCCC TCAACCCCAG 
CTGGACTTCC AGGACTCTCA AGAGAGCCTC TCTGACGAGG AGTATGCCCA GATGCAGCAC 
TTAGCTCGGC TTGCAGAAAA CCTCATCAGC AAACTTAATG ACCTGAGCAA GATCCACCAG 
CATGTGTCTG TCCTCATGGG TTCTGTGAAT GCCAGCGCTA ACATCCTGAA TGATATATTT 
TACGGCCAAG ACAGTGGCAA TGAGATGGGT GCAGCTTCCT GTATTCCCAT TGAAACTCTA 
AGCAGAAATG ATGCCGTAGA TGTTACATTT AGTCTTAAAG GTCTCTACGA TGACACCACA 
GCTTTCCTGG ATGAAAAGCT GCTGAGAAGT GCTGAGGATG AGACTGCACT ACAAAGCGCC 
CTGGATGCCA TGAATAAGGA GTTCAAAAAG CTATCTGAGA TTGACTGGAT GAATCCAATC 
TTTGTTCCAG AGGAAAAATC TTCGGACACT GACAGTAGAA GCCTCAGGCT GAAAATTAAG 
TTCCCCAAAT TGGGAAAGAA AAAGGTAGAA GAGGAACGCA AGCCTAAATC AGGCCAGAGT 
GTCCAGAGTT TTATTGGCAA TTTATGGCAC CGCAGACATC GTGAAGATGA AGCAGAGGGT 
GATGATCCTC TAACACCATC GAGATCTCAA CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKK ( NM_001013742.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001013764.1 Homo sapiens diacylglycerol kinase kappa (DGKK), mRNA.

Price & Availability↑

CloneID	OHu21039
Clone ID Related Accession (Same CDS sequence)	NM_001013742.3 , NM_001013742.4
Accession Version	NM_001013742.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3816bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	diacylglycerol kinase kappa
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC233286.2, AB183864.1, AC239396.3 and AI591038.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_001013742.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AB183864.1, BC137319.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145544, SAMEA2162841 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000611977.2/ ENSP00000477515.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGACCGCG GAGCTGCCGC AGCCCAGGGC ACTGCCCCGC CTCAGGATGG AGAGCAGCCC 
GCTGAGTCTC CAGAGCCTCC GCCGCCTTGG CCGCCGCCGC CACCACCACC GGCTCCGCCG 
CCGGCTCCGC CGCTGCTCTC CGAGGCTTCG CCAGAACCCA TACCAGAGCC CTGTCCAGAG 
CTTGCTCCAG GTCCCTGTCC AGAGGCGACC TCAGAATCAG CCACAGAACT GTACACAGAA 
CCGACCCCAG AACCAGCCAC AGAGCCGGCC TCAGAACCGG CCCCAGAACC TGCCACAGAG 
CCGGCCCCAG AACCTGCCAC AGAGCCGGCC CCAGAACCGG CCCCAGAACC TGCCACAGAG 
TCTGCCCCAG AGCCGACTCC AGAACCTGCC CTAGAGTCGG TCCCAGAGCC GGCCCCAGAG 
CTGACTCCAG AAGTTGCCCC AGAGCTGGCC CCAGAGCCGA CCCCAGAACC TGTGACAGAG 
CTGGCCCCAG AGTTCTGCCC TGAGGCGGCT CCAGAGTTCC GTCCAAGTCC AGCACCATGT 
CTATTGCAAT GTCCGGTGGA CACTCGAGAG AGAGGTCTAA AGACCTCGCC ATCGCCATCG 
CCATCGCCAT CGCCCAGAAC GCCAATGTCG TGGTCCAGAA TAAAGAAAAT ATTGAAGGAA 
GGACCTATGC TGAAGAACTG TAACTCTTTC AAGAGATGGA AGCTTAGATA TTTTCTGGTT 
CAAGGACAGA AGCTCTACTT TGCACACCAT CCCGCGTTTG CACACTTTGA AACGATTGAT 
CTGTCTCAAG CCACTGTGGC AGAAAGCAGC TGTAGAAACC TTTGCCACAG TTTTTGTGTT 
ATTACACCAC AACGAAAAAT CACTCTGGCT GCACCCAACC GGAAAGACAT GGAAGAATGG 
ATTAACATCA TAAAAACCAT CCAACAGGGA GAAATTTATA AGATACCTGC AGCAGAAAAC 
AACCCTTTTC TTGTTGGAAT GCATTGTTGG TACTCCAGTT ACAGCCACCG GACCCAGCAC 
TGCAATGTTT GTCGAGAGAG CATTCCTGCC TTATCTAGAG ATGCCATCAT CTGTGAAGTG 
TGCAAAGTGA AATCTCACAG ATTGTGTGCT TTGAGAGCAA GCAAAGACTG CAAGTGGAAT 
ACATTGTCTA TCACTGATGA CCTCCTTCTG CCTGCAGATG AAGTAAACAT GCCCCATCAA 
TGGGTAGAAG GAAACATGCC TGTCAGCTCT CAGTGTGCAG TGTGTCATGA GAGCTGTGGC 
AGTTATCAAA GACTTCAAGA CTTCCGCTGC CTGTGGTGTA ATTCTACGGT GCATGATGAC 
TGTAGGAGAC GGTTTTCCAA GGAATGTTGC TTCAGAAGCC ATCGCTCATC AGTCATTCCT 
CCCACTGCTC TAAGCGACCC CAAAGGCGAT GGCCAATTAG TAGTATCTTC AGACTTCTGG 
AATCTTGATT GGTCATCAGC CTGTTCATGT CCCTTGCTCA TCTTCATCAA CTCCAAAAGT 
GGCGATCATC AGGGGATCGT CTTCCTCCGA AAATTCAAGC AATACCTTAA CCCATCTCAA 
GTGTTCGACT TATTGAAGGG TGGACCTGAA GCAGGGCTGT CTATGTTCAA GAACTTTGCT 
CGCTTTCGCA TTCTGGTTTG TGGTGGAGAT GGCAGCGTGA GCTGGGTCTT ATCTCTGATT 
GATGCCTTTG GATTACATGA AAAGTGTCAG TTGGCAGTCA TCCCACTTGG AACCGGCAAT 
GATCTGGCTC GTGTTCTGGG CTGGGGTGCA TTCTGGAACA AAAGCAAGTC ACCTCTGGAC 
ATCCTCAACA GAGTGGAGCA GGCTAGTGTG AGGATCCTAG ACAGATGGAG TGTGATGATT 
CGTGAGACTC CCAGACAAAC CCCGCTGCTA AAAGGACAGG TTGAAATGGA TGTACCACGA 
TTTGAGGCTG CTGCCATCCA ACACTTAGAA TCTGCAGCCA CCGAGTTGAA CAAAATCCTG 
AAGGCCAAGT ACCCCACAGA GATGATCATC GCAACCAGAT TCTTGTGTTC AGCTGTGGAA 
GATTTTGTGG TTGATATTGT AAAGGCCTGG GGTCAGATAA AACAGAACAA CACTGCAATA 
GTGTCTGTGA TTTTGAAAAG TGACTTAATG TATGATAGGC TCAGTGTCCT GATCGATGTC 
CTGGCTGAGG AGGCAGCAGC TACTTCTGCT GAAAAAAGTG CTACAGAATA TGCAGACAGC 
AGCAAGGCAG ATAGGAAGCC CTTCATTCCT CAAATAGACC ACATAGCCAA GTGCAAGTTG 
GAGCTGGCTA CAAAGGCCCA GAGTCTCCAG AAATCCTTGA AACTCATCAT ATTTCAAGTT 
GAACAAGCTC TGGATGAGGA AAGCAGACAG ACAATATCTG TTAAGAACTT TAGTTCAACT 
TTCTTCCTGG AAGATGACCC AGAAGATATT AACCAGACAA GCCCACGACG CCGTTCTCGT 
CGTGGCACTT TGTCTTCTAT ATCTTCTCTC AAAAGTGAGG ACCTGGACAA CCTTAACTTG 
GATCACTTAC ATTTTACACC TGAATCTATA CGCTTCAAAG AAAAATGTGT CATGAACAAC 
TACTTCGGAA TTGGACTGGA TGCTAAAATT TCTCTGGACT TCAACACCAG AAGAGATGAA 
CACCCAGGGC AATACAATAG CCGCCTTAAG AACAAGATGT GGTATGGCCT TCTGGGAACC 
AAAGAACTTT TGCAGCGCTC TTACAGGAAA CTGGAAGAAC GAGTGCATTT GGAGTGTGAT 
GGAGAAACCA TCTCCTTGCC AAACCTGCAA GGCATTGTAG TGCTCAACAT TACCAGCTAT 
GCTGGAGGTA TCAACTTCTG GGGAAGCAAC ACAGCAACCA CGGAATATGA GGCTCCTGCA 
ATCGATGATG GGAAACTGGA GGTGGTGGCA ATCTTTGGTT CTGTGCAGAT GGCAATGTCC 
CGTATCATCA ACCTGCATCA TCATCGCATT GCCCAGTGCC ATGAGGTGAT GATAACCATT 
GATGGTGAAG AAGGTATCCC AGTGCAGGTG GATGGGGAGG CCTGGATTCA GAGACCAGGC 
CTTATCAAAA TTAGATACAA GAACGCTGCC CAGATGCTGA CAAGAGATCG GGACTTTGAG 
AACTCAATGA AAATGTGGGA ATACAAGCAT ACTGAAATTC AAGCTGCCCC TCAACCCCAG 
CTGGACTTCC AGGACTCTCA AGAGAGCCTC TCTGACGAGG AGTATGCCCA GATGCAGCAC 
TTAGCTCGGC TTGCAGAAAA CCTCATCAGC AAACTTAATG ACCTGAGCAA GATCCACCAG 
CATGTGTCTG TCCTCATGGG TTCTGTGAAT GCCAGCGCTA ACATCCTGAA TGATATATTT 
TACGGCCAAG ACAGTGGCAA TGAGATGGGT GCAGCTTCCT GTATTCCCAT TGAAACTCTA 
AGCAGAAATG ATGCCGTAGA TGTTACATTT AGTCTTAAAG GTCTCTACGA TGACACCACA 
GCTTTCCTGG ATGAAAAGCT GCTGAGAAGT GCTGAGGATG AGACTGCACT ACAAAGCGCC 
CTGGATGCCA TGAATAAGGA GTTCAAAAAG CTATCTGAGA TTGACTGGAT GAATCCAATC 
TTTGTTCCAG AGGAAAAATC TTCGGACACT GACAGTAGAA GCCTCAGGCT GAAAATTAAG 
TTCCCCAAAT TGGGAAAGAA AAAGGTAGAA GAGGAACGCA AGCCTAAATC AGGCCAGAGT 
GTCCAGAGTT TTATTGGCAA TTTATGGCAC CGCAGACATC GTGAAGATGA AGCAGAGGGT 
GATGATCCTC TAACACCATC GAGATCTCAA CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001013764.1
CDS	148..3963
Translation	MDRGAAAAQGTAPPQDGEQPAESPEPPPPWPPPPPPPAPPPAPPLLSEASPEPIPEPCPELAPGPCPEATSESATELYTEPTPEPATEPASEPAPEPATEPAPEPATEPAPEPAPEPATESAPEPTPEPALESVPEPAPELTPEVAPELAPEPTPEPVTELAPEFCPEAAPEFRPSPAPCLLQCPVDTRERGLKTSPSPSPSPSPRTPMSWSRIKKILKEGPMLKNCNSFKRWKLRYFLVQGQKLYFAHHPAFAHFETIDLSQATVAESSCRNLCHSFCVITPQRKITLAAPNRKDMEEWINIIKTIQQGEIYKIPAAENNPFLVGMHCWYSSYSHRTQHCNVCRESIPALSRDAIICEVCKVKSHRLCALRASKDCKWNTLSITDDLLLPADEVNMPHQWVEGNMPVSSQCAVCHESCGSYQRLQDFRCLWCNSTVHDDCRRRFSKECCFRSHRSSVIPPTALSDPKGDGQLVVSSDFWNLDWSSACSCPLLIFINSKSGDHQGIVFLRKFKQYLNPSQVFDLLKGGPEAGLSMFKNFARFRILVCGGDGSVSWVLSLIDAFGLHEKCQLAVIPLGTGNDLARVLGWGAFWNKSKSPLDILNRVEQASVRILDRWSVMIRETPRQTPLLKGQVEMDVPRFEAAAIQHLESAATELNKILKAKYPTEMIIATRFLCSAVEDFVVDIVKAWGQIKQNNTAIVSVILKSDLMYDRLSVLIDVLAEEAAATSAEKSATEYADSSKADRKPFIPQIDHIAKCKLELATKAQSLQKSLKLIIFQVEQALDEESRQTISVKNFSSTFFLEDDPEDINQTSPRRRSRRGTLSSISSLKSEDLDNLNLDHLHFTPESIRFKEKCVMNNYFGIGLDAKISLDFNTRRDEHPGQYNSRLKNKMWYGLLGTKELLQRSYRKLEERVHLECDGETISLPNLQGIVVLNITSYAGGINFWGSNTATTEYEAPAIDDGKLEVVAIFGSVQMAMSRIINLHHHRIAQCHEVMITIDGEEGIPVQVDGEAWIQRPGLIKIRYKNAAQMLTRDRDFENSMKMWEYKHTEIQAAPQPQLDFQDSQESLSDEEYAQMQHLARLAENLISKLNDLSKIHQHVSVLMGSVNASANILNDIFYGQDSGNEMGAASCIPIETLSRNDAVDVTFSLKGLYDDTTAFLDEKLLRSAEDETALQSALDAMNKEFKKLSEIDWMNPIFVPEEKSSDTDSRSLRLKIKFPKLGKKKVEEERKPKSGQSVQSFIGNLWHRRHREDEAEGDDPLTPSRSQL

Target ORF information:

RefSeq Version	NM_001013742.4
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase kappa (DGKK), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001013742.4

ORF Insert Sequence:

ATGGACCGCG GAGCTGCCGC AGCCCAGGGC ACTGCCCCGC CTCAGGATGG AGAGCAGCCC 
GCTGAGTCTC CAGAGCCTCC GCCGCCTTGG CCGCCGCCGC CACCACCACC GGCTCCGCCG 
CCGGCTCCGC CGCTGCTCTC CGAGGCTTCG CCAGAACCCA TACCAGAGCC CTGTCCAGAG 
CTTGCTCCAG GTCCCTGTCC AGAGGCGACC TCAGAATCAG CCACAGAACT GTACACAGAA 
CCGACCCCAG AACCAGCCAC AGAGCCGGCC TCAGAACCGG CCCCAGAACC TGCCACAGAG 
CCGGCCCCAG AACCTGCCAC AGAGCCGGCC CCAGAACCGG CCCCAGAACC TGCCACAGAG 
TCTGCCCCAG AGCCGACTCC AGAACCTGCC CTAGAGTCGG TCCCAGAGCC GGCCCCAGAG 
CTGACTCCAG AAGTTGCCCC AGAGCTGGCC CCAGAGCCGA CCCCAGAACC TGTGACAGAG 
CTGGCCCCAG AGTTCTGCCC TGAGGCGGCT CCAGAGTTCC GTCCAAGTCC AGCACCATGT 
CTATTGCAAT GTCCGGTGGA CACTCGAGAG AGAGGTCTAA AGACCTCGCC ATCGCCATCG 
CCATCGCCAT CGCCCAGAAC GCCAATGTCG TGGTCCAGAA TAAAGAAAAT ATTGAAGGAA 
GGACCTATGC TGAAGAACTG TAACTCTTTC AAGAGATGGA AGCTTAGATA TTTTCTGGTT 
CAAGGACAGA AGCTCTACTT TGCACACCAT CCCGCGTTTG CACACTTTGA AACGATTGAT 
CTGTCTCAAG CCACTGTGGC AGAAAGCAGC TGTAGAAACC TTTGCCACAG TTTTTGTGTT 
ATTACACCAC AACGAAAAAT CACTCTGGCT GCACCCAACC GGAAAGACAT GGAAGAATGG 
ATTAACATCA TAAAAACCAT CCAACAGGGA GAAATTTATA AGATACCTGC AGCAGAAAAC 
AACCCTTTTC TTGTTGGAAT GCATTGTTGG TACTCCAGTT ACAGCCACCG GACCCAGCAC 
TGCAATGTTT GTCGAGAGAG CATTCCTGCC TTATCTAGAG ATGCCATCAT CTGTGAAGTG 
TGCAAAGTGA AATCTCACAG ATTGTGTGCT TTGAGAGCAA GCAAAGACTG CAAGTGGAAT 
ACATTGTCTA TCACTGATGA CCTCCTTCTG CCTGCAGATG AAGTAAACAT GCCCCATCAA 
TGGGTAGAAG GAAACATGCC TGTCAGCTCT CAGTGTGCAG TGTGTCATGA GAGCTGTGGC 
AGTTATCAAA GACTTCAAGA CTTCCGCTGC CTGTGGTGTA ATTCTACGGT GCATGATGAC 
TGTAGGAGAC GGTTTTCCAA GGAATGTTGC TTCAGAAGCC ATCGCTCATC AGTCATTCCT 
CCCACTGCTC TAAGCGACCC CAAAGGCGAT GGCCAATTAG TAGTATCTTC AGACTTCTGG 
AATCTTGATT GGTCATCAGC CTGTTCATGT CCCTTGCTCA TCTTCATCAA CTCCAAAAGT 
GGCGATCATC AGGGGATCGT CTTCCTCCGA AAATTCAAGC AATACCTTAA CCCATCTCAA 
GTGTTCGACT TATTGAAGGG TGGACCTGAA GCAGGGCTGT CTATGTTCAA GAACTTTGCT 
CGCTTTCGCA TTCTGGTTTG TGGTGGAGAT GGCAGCGTGA GCTGGGTCTT ATCTCTGATT 
GATGCCTTTG GATTACATGA AAAGTGTCAG TTGGCAGTCA TCCCACTTGG AACCGGCAAT 
GATCTGGCTC GTGTTCTGGG CTGGGGTGCA TTCTGGAACA AAAGCAAGTC ACCTCTGGAC 
ATCCTCAACA GAGTGGAGCA GGCTAGTGTG AGGATCCTAG ACAGATGGAG TGTGATGATT 
CGTGAGACTC CCAGACAAAC CCCGCTGCTA AAAGGACAGG TTGAAATGGA TGTACCACGA 
TTTGAGGCTG CTGCCATCCA ACACTTAGAA TCTGCAGCCA CCGAGTTGAA CAAAATCCTG 
AAGGCCAAGT ACCCCACAGA GATGATCATC GCAACCAGAT TCTTGTGTTC AGCTGTGGAA 
GATTTTGTGG TTGATATTGT AAAGGCCTGG GGTCAGATAA AACAGAACAA CACTGCAATA 
GTGTCTGTGA TTTTGAAAAG TGACTTAATG TATGATAGGC TCAGTGTCCT GATCGATGTC 
CTGGCTGAGG AGGCAGCAGC TACTTCTGCT GAAAAAAGTG CTACAGAATA TGCAGACAGC 
AGCAAGGCAG ATAGGAAGCC CTTCATTCCT CAAATAGACC ACATAGCCAA GTGCAAGTTG 
GAGCTGGCTA CAAAGGCCCA GAGTCTCCAG AAATCCTTGA AACTCATCAT ATTTCAAGTT 
GAACAAGCTC TGGATGAGGA AAGCAGACAG ACAATATCTG TTAAGAACTT TAGTTCAACT 
TTCTTCCTGG AAGATGACCC AGAAGATATT AACCAGACAA GCCCACGACG CCGTTCTCGT 
CGTGGCACTT TGTCTTCTAT ATCTTCTCTC AAAAGTGAGG ACCTGGACAA CCTTAACTTG 
GATCACTTAC ATTTTACACC TGAATCTATA CGCTTCAAAG AAAAATGTGT CATGAACAAC 
TACTTCGGAA TTGGACTGGA TGCTAAAATT TCTCTGGACT TCAACACCAG AAGAGATGAA 
CACCCAGGGC AATACAATAG CCGCCTTAAG AACAAGATGT GGTATGGCCT TCTGGGAACC 
AAAGAACTTT TGCAGCGCTC TTACAGGAAA CTGGAAGAAC GAGTGCATTT GGAGTGTGAT 
GGAGAAACCA TCTCCTTGCC AAACCTGCAA GGCATTGTAG TGCTCAACAT TACCAGCTAT 
GCTGGAGGTA TCAACTTCTG GGGAAGCAAC ACAGCAACCA CGGAATATGA GGCTCCTGCA 
ATCGATGATG GGAAACTGGA GGTGGTGGCA ATCTTTGGTT CTGTGCAGAT GGCAATGTCC 
CGTATCATCA ACCTGCATCA TCATCGCATT GCCCAGTGCC ATGAGGTGAT GATAACCATT 
GATGGTGAAG AAGGTATCCC AGTGCAGGTG GATGGGGAGG CCTGGATTCA GAGACCAGGC 
CTTATCAAAA TTAGATACAA GAACGCTGCC CAGATGCTGA CAAGAGATCG GGACTTTGAG 
AACTCAATGA AAATGTGGGA ATACAAGCAT ACTGAAATTC AAGCTGCCCC TCAACCCCAG 
CTGGACTTCC AGGACTCTCA AGAGAGCCTC TCTGACGAGG AGTATGCCCA GATGCAGCAC 
TTAGCTCGGC TTGCAGAAAA CCTCATCAGC AAACTTAATG ACCTGAGCAA GATCCACCAG 
CATGTGTCTG TCCTCATGGG TTCTGTGAAT GCCAGCGCTA ACATCCTGAA TGATATATTT 
TACGGCCAAG ACAGTGGCAA TGAGATGGGT GCAGCTTCCT GTATTCCCAT TGAAACTCTA 
AGCAGAAATG ATGCCGTAGA TGTTACATTT AGTCTTAAAG GTCTCTACGA TGACACCACA 
GCTTTCCTGG ATGAAAAGCT GCTGAGAAGT GCTGAGGATG AGACTGCACT ACAAAGCGCC 
CTGGATGCCA TGAATAAGGA GTTCAAAAAG CTATCTGAGA TTGACTGGAT GAATCCAATC 
TTTGTTCCAG AGGAAAAATC TTCGGACACT GACAGTAGAA GCCTCAGGCT GAAAATTAAG 
TTCCCCAAAT TGGGAAAGAA AAAGGTAGAA GAGGAACGCA AGCCTAAATC AGGCCAGAGT 
GTCCAGAGTT TTATTGGCAA TTTATGGCAC CGCAGACATC GTGAAGATGA AGCAGAGGGT 
GATGATCCTC TAACACCATC GAGATCTCAA CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKK ( XM_017029268.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884757.1 Homo sapiens diacylglycerol kinase kappa (DGKK), transcript variant X1, mRNA.

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CloneID	OHu99152
Clone ID Related Accession (Same CDS sequence)	XM_017029268.1 , XM_017029268.2
Accession Version	XM_017029268.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase kappa isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCGCG GAGCTGCCGC AGCCCAGGGC ACTGCCCCGC CTCAGGATGG AGAGCAGCCC 
GCTGAGTCTC CAGAGCCTCC GCCGCCTTGG CCGCCGCCGC CACCACCACC GGCTCCGCCG 
CCGGCTCCGC CGCTGCTCTC CGAGGCTTCG CCAGAACCCA TACCAGAGCC CTGTCCAGAG 
CTTGCTCCAG GTCCCTGTCC AGAGGCGACC TCAGAATCAG CCACAGAACT GTACACAGAA 
CCGACCCCAG AACCAGCCAC AGAGCCGGCC TCAGAACCGG CCCCAGAACC TGCCACAGAG 
CCGGCCCCAG AACCTGCCAC AGAGCCGGCC CCAGAACCGG CCCCAGAACC TGCCACAGAG 
TCTGCCCCAG AGCCGACTCC AGAACCTGCC CTAGAGTCGG TCCCAGAGCC GGCCCCAGAG 
CTGACTCCAG AAGTTGCCCC AGAGCTGGCC CCAGAGCCGA CCCCAGAACC TGTGACAGAG 
CTGGCCCCAG AGTTCTGCCC TGAGGCGGCT CCAGAGTTCC GTCCAAGTCC AGCACCATGT 
CTATTGCAAT GTCCGGTGGA CACTCGAGAG AGAGGTCTAA AGACCTCGCC ATCGCCATCG 
CCATCGCCAT CGCCCAGAAC GCCAATGTCG TGGTCCAGAA TAAAGAAAAT ATTGAAGGAA 
GGACCTATGC TGAAGAACTG TAACTCTTTC AAGAGATGGA AGCTTAGATA TTTTCTGGTT 
CAAGGACAGA AGCTCTACTT TGCACACCAT CCCGCGTTTG CACACTTTGA AACGATTGAT 
CTGTCTCAAG CCACTGTGGC AGAAAGCAGC TGTAGAAACC TTTGCCACAG TTTTTGTGTT 
ATTACACCAC AACGAAAAAT CACTCTGGCT GCACCCAACC GGAAAGACAT GGAAGAATGG 
ATTAACATCA TAAAAACCAT CCAACAGGGA GAAATTTATA AGATACCTGC AGCAGAAAAC 
AACCCTTTTC TTGTTGGAAT GCATTGTTGG TACTCCAGTT ACAGCCACCG GACCCAGCAC 
TGCAATGTTT GTCGAGAGAG CATTCCTGCC TTATCTAGAG ATGCCATCAT CTGTGAAGTG 
TGCAAAGTGA AATCTCACAG ATTGTGTGCT TTGAGAGCAA GCAAAGACTG CAAGTGGAAT 
ACATTGTCTA TCACTGATGA CCTCCTTCTG CCTGCAGATG AAGTAAACAT GCCCCATCAA 
TGGGTAGAAG GAAACATGCC TGTCAGCTCT CAGTGTGCAG TGTGTCATGA GAGCTGTGGC 
AGTTATCAAA GACTTCAAGA CTTCCGCTGC CTGTGGTGTA ATTCTACGGT GCATGATGAC 
TGTAGGAGAC GGTTTTCCAA GGAATGTTGC TTCAGAAGCC ATCGCTCATC AGTCATTCCT 
CCCACTGCTC TAAGCGACCC CAAAGGCGAT GGCCAATTAG TAGTATCTTC AGACTTCTGG 
AATCTTGATT GGTCATCAGC CTGTTCATGT CCCTTGCTCA TCTTCATCAA CTCCAAAAGT 
GGCGATCATC AGGGGATCGT CTTCCTCCGA AAATTCAAGC AATACCTTAA CCCATCTCAA 
GTGTTCGACT TATTGAAGGG TGGACCTGAA GCAGGGCTGT CTATGTTCAA GAACTTTGCT 
CGCTTTCGCA TTCTGGTTTG TGGTGGAGAT GGCAGCGTGA GCTGGGTCTT ATCTCTGATT 
GATGCCTTTG GATTACATGA AAAGTGTCAG TTGGCAGTCA TCCCACTTGG AACCGGCAAT 
GATCTGGCTC GTGTTCTGGG CTGGGGTGCA TTCTGGAACA AAAGCAAGTC ACCTCTGGAC 
ATCCTCAACA GAGTGGAGCA GGCTAGTGTG AGGATCCTAG ACAGATGGAG TGTGATGATT 
CGTGAGACTC CCAGACAAAC CCCGCTGCTA AAAGGACAGG TTGAAATGGA TGTACCACGA 
TTTGAGGCTG CTGCCATCCA ACACTTAGAA TCTGCAGCCA CCGAGTTGAA CAAAATCCTG 
AAGGCCAAGT ACCCCACAGA GATGATCATC GCAACCAGAT TCTTGTGTTC AGCTGTGGAA 
GATTTTGTGG TTGATATTGT AAAGGCCTGG GGTCAGATAA AACAGAACAA CACTGCAATA 
GTGTCTGTGA TTTTGAAAAG TGACTTAATG TATGATAGGC TCAGTGTCCT GATCGATGTC 
CTGGCTGAGG AGGCAGCAGC TACTTCTGCT GAAAAAAGTG CTACAGAATA TGCAGACAGC 
AGCAAGGCAG ATAGGAAGCC CTTCATTCCT CAAATAGACC ACATAGCCAA GTGCAAGTTG 
GAGCTGGCTA CAAAGGCCCA GAGTCTCCAG AAATCCTTGA AACTCATCAT ATTTCAAGTT 
GAACAAGCTC TGGATGAGGA AAGCAGACAG ACAATATCTG TTAAGAACTT TAGTTCAACT 
TTCTTCCTGG AAGATGACCC AGAAGATATT AACCAGACAA GCCCACGACG CCGTTCTCGT 
CGTGGCACTT TGTCTTCTAT ATCTTCTCTC AAAAGTGAGG ACCTGGACAA CCTTAACTTG 
GATCACTTAC ATTTTACACC TGAATCTATA CGCTTCAAAG AAAAATGTGT CATGAACAAC 
TACTTCGGAA TTGGACTGGA TGCTAAAATT TCTCTGGACT TCAACACCAG AAGAGATGAA 
CACCCAGGGC AATACAATAG CCGCCTTAAG AACAAGATGT GGTATGGCCT TCTGGGAACC 
AAAGAACTTT TGCAGCGCTC TTACAGGAAA CTGGAAGAAC GAGTGCATTT GGAGTGTGAT 
GGAGAAACCA TCTCCTTGCC AAACCTGCAA GGCATTGTAG TGCTCAACAT TACCAGCTAT 
GCTGGAGGTA TCAACTTCTG GGGAAGCAAC ACAGCAACCA CGGAATATGA GGCTCCTGCA 
ATCGATGATG GGAAACTGGA GGTGGTGGCA ATCTTTGGTT CTGTGCAGAT GGCAATGTCC 
CGTATCATCA ACCTGCATCA TCATCGCATT GCCCAGTGCC ATGAGGTGAT GATAACCATT 
GATGGTGAAG AAGGTATCCC AGTGCAGGTG GATGGGGAGG CCTGGATTCA GAGACCAGGC 
CTTATCAAAA TTAGATACAA GAACGCTGCC CAGATGCTGA CAAGAGATCG GGACTTTGAG 
AACTCAATGA AAATGTGGGA ATACAAGCAT ACTGAAATTC AAGCTGCCCC TCAACCCCAG 
CTGGACTTCC AGGACTCTCA AGAGAGCCTC TCTGACGAGG AGTATGCCCA GATGCAGCAC 
TTAGCTCGGC TTGCAGAAAA CCTCATCAGC AAACTTAATG ACCTGAGCAA GATCCACCAG 
CATGTGTCTG TCCTCATGGG TTCTGTGAAT GCCAGCGCTA ACATCCTGAA TGATATATTT 
TACGGCCAAG ACAGTGGCAA TGAGATGGGT GCAGCTTCCT GTATTCCCAT TGAACTGAGA 
AGTGCTGAGG ATGAGACTGC ACTACAAAGC GCCCTGGATG CCATGAATAA GGAGTTCAAA 
AAGCTATCTG AGATTGACTG GATGAATCCA ATCTTTGTTC CAGAGGAAAA ATCTTCGGAC 
ACTGACAGTA GAAGCCTCAG GCTGAAAATT AAGTTCCCCA AATTGGGAAA GAAAAAGGTA 
GAAGAGGAAC GCAAGCCTAA ATCAGGCCAG AGTGTCCAGA GTTTTATTGG CAATTTATGG 
CACCGCAGAC ATCGTGAAGA TGAAGCAGAG GGTGATGATC CTCTAACACC ATCGAGATCT 
CAACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884757.1
CDS	173..3901
Translation	MDRGAAAAQGTAPPQDGEQPAESPEPPPPWPPPPPPPAPPPAPPLLSEASPEPIPEPCPELAPGPCPEATSESATELYTEPTPEPATEPASEPAPEPATEPAPEPATEPAPEPAPEPATESAPEPTPEPALESVPEPAPELTPEVAPELAPEPTPEPVTELAPEFCPEAAPEFRPSPAPCLLQCPVDTRERGLKTSPSPSPSPSPRTPMSWSRIKKILKEGPMLKNCNSFKRWKLRYFLVQGQKLYFAHHPAFAHFETIDLSQATVAESSCRNLCHSFCVITPQRKITLAAPNRKDMEEWINIIKTIQQGEIYKIPAAENNPFLVGMHCWYSSYSHRTQHCNVCRESIPALSRDAIICEVCKVKSHRLCALRASKDCKWNTLSITDDLLLPADEVNMPHQWVEGNMPVSSQCAVCHESCGSYQRLQDFRCLWCNSTVHDDCRRRFSKECCFRSHRSSVIPPTALSDPKGDGQLVVSSDFWNLDWSSACSCPLLIFINSKSGDHQGIVFLRKFKQYLNPSQVFDLLKGGPEAGLSMFKNFARFRILVCGGDGSVSWVLSLIDAFGLHEKCQLAVIPLGTGNDLARVLGWGAFWNKSKSPLDILNRVEQASVRILDRWSVMIRETPRQTPLLKGQVEMDVPRFEAAAIQHLESAATELNKILKAKYPTEMIIATRFLCSAVEDFVVDIVKAWGQIKQNNTAIVSVILKSDLMYDRLSVLIDVLAEEAAATSAEKSATEYADSSKADRKPFIPQIDHIAKCKLELATKAQSLQKSLKLIIFQVEQALDEESRQTISVKNFSSTFFLEDDPEDINQTSPRRRSRRGTLSSISSLKSEDLDNLNLDHLHFTPESIRFKEKCVMNNYFGIGLDAKISLDFNTRRDEHPGQYNSRLKNKMWYGLLGTKELLQRSYRKLEERVHLECDGETISLPNLQGIVVLNITSYAGGINFWGSNTATTEYEAPAIDDGKLEVVAIFGSVQMAMSRIINLHHHRIAQCHEVMITIDGEEGIPVQVDGEAWIQRPGLIKIRYKNAAQMLTRDRDFENSMKMWEYKHTEIQAAPQPQLDFQDSQESLSDEEYAQMQHLARLAENLISKLNDLSKIHQHVSVLMGSVNASANILNDIFYGQDSGNEMGAASCIPIELRSAEDETALQSALDAMNKEFKKLSEIDWMNPIFVPEEKSSDTDSRSLRLKIKFPKLGKKKVEEERKPKSGQSVQSFIGNLWHRRHREDEAEGDDPLTPSRSQL

Target ORF information:

RefSeq Version	XM_017029268.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase kappa (DGKK), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029268.1

ORF Insert Sequence:

ATGGACCGCG GAGCTGCCGC AGCCCAGGGC ACTGCCCCGC CTCAGGATGG AGAGCAGCCC 
GCTGAGTCTC CAGAGCCTCC GCCGCCTTGG CCGCCGCCGC CACCACCACC GGCTCCGCCG 
CCGGCTCCGC CGCTGCTCTC CGAGGCTTCG CCAGAACCCA TACCAGAGCC CTGTCCAGAG 
CTTGCTCCAG GTCCCTGTCC AGAGGCGACC TCAGAATCAG CCACAGAACT GTACACAGAA 
CCGACCCCAG AACCAGCCAC AGAGCCGGCC TCAGAACCGG CCCCAGAACC TGCCACAGAG 
CCGGCCCCAG AACCTGCCAC AGAGCCGGCC CCAGAACCGG CCCCAGAACC TGCCACAGAG 
TCTGCCCCAG AGCCGACTCC AGAACCTGCC CTAGAGTCGG TCCCAGAGCC GGCCCCAGAG 
CTGACTCCAG AAGTTGCCCC AGAGCTGGCC CCAGAGCCGA CCCCAGAACC TGTGACAGAG 
CTGGCCCCAG AGTTCTGCCC TGAGGCGGCT CCAGAGTTCC GTCCAAGTCC AGCACCATGT 
CTATTGCAAT GTCCGGTGGA CACTCGAGAG AGAGGTCTAA AGACCTCGCC ATCGCCATCG 
CCATCGCCAT CGCCCAGAAC GCCAATGTCG TGGTCCAGAA TAAAGAAAAT ATTGAAGGAA 
GGACCTATGC TGAAGAACTG TAACTCTTTC AAGAGATGGA AGCTTAGATA TTTTCTGGTT 
CAAGGACAGA AGCTCTACTT TGCACACCAT CCCGCGTTTG CACACTTTGA AACGATTGAT 
CTGTCTCAAG CCACTGTGGC AGAAAGCAGC TGTAGAAACC TTTGCCACAG TTTTTGTGTT 
ATTACACCAC AACGAAAAAT CACTCTGGCT GCACCCAACC GGAAAGACAT GGAAGAATGG 
ATTAACATCA TAAAAACCAT CCAACAGGGA GAAATTTATA AGATACCTGC AGCAGAAAAC 
AACCCTTTTC TTGTTGGAAT GCATTGTTGG TACTCCAGTT ACAGCCACCG GACCCAGCAC 
TGCAATGTTT GTCGAGAGAG CATTCCTGCC TTATCTAGAG ATGCCATCAT CTGTGAAGTG 
TGCAAAGTGA AATCTCACAG ATTGTGTGCT TTGAGAGCAA GCAAAGACTG CAAGTGGAAT 
ACATTGTCTA TCACTGATGA CCTCCTTCTG CCTGCAGATG AAGTAAACAT GCCCCATCAA 
TGGGTAGAAG GAAACATGCC TGTCAGCTCT CAGTGTGCAG TGTGTCATGA GAGCTGTGGC 
AGTTATCAAA GACTTCAAGA CTTCCGCTGC CTGTGGTGTA ATTCTACGGT GCATGATGAC 
TGTAGGAGAC GGTTTTCCAA GGAATGTTGC TTCAGAAGCC ATCGCTCATC AGTCATTCCT 
CCCACTGCTC TAAGCGACCC CAAAGGCGAT GGCCAATTAG TAGTATCTTC AGACTTCTGG 
AATCTTGATT GGTCATCAGC CTGTTCATGT CCCTTGCTCA TCTTCATCAA CTCCAAAAGT 
GGCGATCATC AGGGGATCGT CTTCCTCCGA AAATTCAAGC AATACCTTAA CCCATCTCAA 
GTGTTCGACT TATTGAAGGG TGGACCTGAA GCAGGGCTGT CTATGTTCAA GAACTTTGCT 
CGCTTTCGCA TTCTGGTTTG TGGTGGAGAT GGCAGCGTGA GCTGGGTCTT ATCTCTGATT 
GATGCCTTTG GATTACATGA AAAGTGTCAG TTGGCAGTCA TCCCACTTGG AACCGGCAAT 
GATCTGGCTC GTGTTCTGGG CTGGGGTGCA TTCTGGAACA AAAGCAAGTC ACCTCTGGAC 
ATCCTCAACA GAGTGGAGCA GGCTAGTGTG AGGATCCTAG ACAGATGGAG TGTGATGATT 
CGTGAGACTC CCAGACAAAC CCCGCTGCTA AAAGGACAGG TTGAAATGGA TGTACCACGA 
TTTGAGGCTG CTGCCATCCA ACACTTAGAA TCTGCAGCCA CCGAGTTGAA CAAAATCCTG 
AAGGCCAAGT ACCCCACAGA GATGATCATC GCAACCAGAT TCTTGTGTTC AGCTGTGGAA 
GATTTTGTGG TTGATATTGT AAAGGCCTGG GGTCAGATAA AACAGAACAA CACTGCAATA 
GTGTCTGTGA TTTTGAAAAG TGACTTAATG TATGATAGGC TCAGTGTCCT GATCGATGTC 
CTGGCTGAGG AGGCAGCAGC TACTTCTGCT GAAAAAAGTG CTACAGAATA TGCAGACAGC 
AGCAAGGCAG ATAGGAAGCC CTTCATTCCT CAAATAGACC ACATAGCCAA GTGCAAGTTG 
GAGCTGGCTA CAAAGGCCCA GAGTCTCCAG AAATCCTTGA AACTCATCAT ATTTCAAGTT 
GAACAAGCTC TGGATGAGGA AAGCAGACAG ACAATATCTG TTAAGAACTT TAGTTCAACT 
TTCTTCCTGG AAGATGACCC AGAAGATATT AACCAGACAA GCCCACGACG CCGTTCTCGT 
CGTGGCACTT TGTCTTCTAT ATCTTCTCTC AAAAGTGAGG ACCTGGACAA CCTTAACTTG 
GATCACTTAC ATTTTACACC TGAATCTATA CGCTTCAAAG AAAAATGTGT CATGAACAAC 
TACTTCGGAA TTGGACTGGA TGCTAAAATT TCTCTGGACT TCAACACCAG AAGAGATGAA 
CACCCAGGGC AATACAATAG CCGCCTTAAG AACAAGATGT GGTATGGCCT TCTGGGAACC 
AAAGAACTTT TGCAGCGCTC TTACAGGAAA CTGGAAGAAC GAGTGCATTT GGAGTGTGAT 
GGAGAAACCA TCTCCTTGCC AAACCTGCAA GGCATTGTAG TGCTCAACAT TACCAGCTAT 
GCTGGAGGTA TCAACTTCTG GGGAAGCAAC ACAGCAACCA CGGAATATGA GGCTCCTGCA 
ATCGATGATG GGAAACTGGA GGTGGTGGCA ATCTTTGGTT CTGTGCAGAT GGCAATGTCC 
CGTATCATCA ACCTGCATCA TCATCGCATT GCCCAGTGCC ATGAGGTGAT GATAACCATT 
GATGGTGAAG AAGGTATCCC AGTGCAGGTG GATGGGGAGG CCTGGATTCA GAGACCAGGC 
CTTATCAAAA TTAGATACAA GAACGCTGCC CAGATGCTGA CAAGAGATCG GGACTTTGAG 
AACTCAATGA AAATGTGGGA ATACAAGCAT ACTGAAATTC AAGCTGCCCC TCAACCCCAG 
CTGGACTTCC AGGACTCTCA AGAGAGCCTC TCTGACGAGG AGTATGCCCA GATGCAGCAC 
TTAGCTCGGC TTGCAGAAAA CCTCATCAGC AAACTTAATG ACCTGAGCAA GATCCACCAG 
CATGTGTCTG TCCTCATGGG TTCTGTGAAT GCCAGCGCTA ACATCCTGAA TGATATATTT 
TACGGCCAAG ACAGTGGCAA TGAGATGGGT GCAGCTTCCT GTATTCCCAT TGAACTGAGA 
AGTGCTGAGG ATGAGACTGC ACTACAAAGC GCCCTGGATG CCATGAATAA GGAGTTCAAA 
AAGCTATCTG AGATTGACTG GATGAATCCA ATCTTTGTTC CAGAGGAAAA ATCTTCGGAC 
ACTGACAGTA GAAGCCTCAG GCTGAAAATT AAGTTCCCCA AATTGGGAAA GAAAAAGGTA 
GAAGAGGAAC GCAAGCCTAA ATCAGGCCAG AGTGTCCAGA GTTTTATTGG CAATTTATGG 
CACCGCAGAC ATCGTGAAGA TGAAGCAGAG GGTGATGATC CTCTAACACC ATCGAGATCT 
CAACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKK ( XM_017029268.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884757.1 Homo sapiens diacylglycerol kinase kappa (DGKK), transcript variant X1, mRNA.

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CloneID	OHu99152
Clone ID Related Accession (Same CDS sequence)	XM_017029268.1 , XM_017029268.2
Accession Version	XM_017029268.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase kappa isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017029268.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCGCG GAGCTGCCGC AGCCCAGGGC ACTGCCCCGC CTCAGGATGG AGAGCAGCCC 
GCTGAGTCTC CAGAGCCTCC GCCGCCTTGG CCGCCGCCGC CACCACCACC GGCTCCGCCG 
CCGGCTCCGC CGCTGCTCTC CGAGGCTTCG CCAGAACCCA TACCAGAGCC CTGTCCAGAG 
CTTGCTCCAG GTCCCTGTCC AGAGGCGACC TCAGAATCAG CCACAGAACT GTACACAGAA 
CCGACCCCAG AACCAGCCAC AGAGCCGGCC TCAGAACCGG CCCCAGAACC TGCCACAGAG 
CCGGCCCCAG AACCTGCCAC AGAGCCGGCC CCAGAACCGG CCCCAGAACC TGCCACAGAG 
TCTGCCCCAG AGCCGACTCC AGAACCTGCC CTAGAGTCGG TCCCAGAGCC GGCCCCAGAG 
CTGACTCCAG AAGTTGCCCC AGAGCTGGCC CCAGAGCCGA CCCCAGAACC TGTGACAGAG 
CTGGCCCCAG AGTTCTGCCC TGAGGCGGCT CCAGAGTTCC GTCCAAGTCC AGCACCATGT 
CTATTGCAAT GTCCGGTGGA CACTCGAGAG AGAGGTCTAA AGACCTCGCC ATCGCCATCG 
CCATCGCCAT CGCCCAGAAC GCCAATGTCG TGGTCCAGAA TAAAGAAAAT ATTGAAGGAA 
GGACCTATGC TGAAGAACTG TAACTCTTTC AAGAGATGGA AGCTTAGATA TTTTCTGGTT 
CAAGGACAGA AGCTCTACTT TGCACACCAT CCCGCGTTTG CACACTTTGA AACGATTGAT 
CTGTCTCAAG CCACTGTGGC AGAAAGCAGC TGTAGAAACC TTTGCCACAG TTTTTGTGTT 
ATTACACCAC AACGAAAAAT CACTCTGGCT GCACCCAACC GGAAAGACAT GGAAGAATGG 
ATTAACATCA TAAAAACCAT CCAACAGGGA GAAATTTATA AGATACCTGC AGCAGAAAAC 
AACCCTTTTC TTGTTGGAAT GCATTGTTGG TACTCCAGTT ACAGCCACCG GACCCAGCAC 
TGCAATGTTT GTCGAGAGAG CATTCCTGCC TTATCTAGAG ATGCCATCAT CTGTGAAGTG 
TGCAAAGTGA AATCTCACAG ATTGTGTGCT TTGAGAGCAA GCAAAGACTG CAAGTGGAAT 
ACATTGTCTA TCACTGATGA CCTCCTTCTG CCTGCAGATG AAGTAAACAT GCCCCATCAA 
TGGGTAGAAG GAAACATGCC TGTCAGCTCT CAGTGTGCAG TGTGTCATGA GAGCTGTGGC 
AGTTATCAAA GACTTCAAGA CTTCCGCTGC CTGTGGTGTA ATTCTACGGT GCATGATGAC 
TGTAGGAGAC GGTTTTCCAA GGAATGTTGC TTCAGAAGCC ATCGCTCATC AGTCATTCCT 
CCCACTGCTC TAAGCGACCC CAAAGGCGAT GGCCAATTAG TAGTATCTTC AGACTTCTGG 
AATCTTGATT GGTCATCAGC CTGTTCATGT CCCTTGCTCA TCTTCATCAA CTCCAAAAGT 
GGCGATCATC AGGGGATCGT CTTCCTCCGA AAATTCAAGC AATACCTTAA CCCATCTCAA 
GTGTTCGACT TATTGAAGGG TGGACCTGAA GCAGGGCTGT CTATGTTCAA GAACTTTGCT 
CGCTTTCGCA TTCTGGTTTG TGGTGGAGAT GGCAGCGTGA GCTGGGTCTT ATCTCTGATT 
GATGCCTTTG GATTACATGA AAAGTGTCAG TTGGCAGTCA TCCCACTTGG AACCGGCAAT 
GATCTGGCTC GTGTTCTGGG CTGGGGTGCA TTCTGGAACA AAAGCAAGTC ACCTCTGGAC 
ATCCTCAACA GAGTGGAGCA GGCTAGTGTG AGGATCCTAG ACAGATGGAG TGTGATGATT 
CGTGAGACTC CCAGACAAAC CCCGCTGCTA AAAGGACAGG TTGAAATGGA TGTACCACGA 
TTTGAGGCTG CTGCCATCCA ACACTTAGAA TCTGCAGCCA CCGAGTTGAA CAAAATCCTG 
AAGGCCAAGT ACCCCACAGA GATGATCATC GCAACCAGAT TCTTGTGTTC AGCTGTGGAA 
GATTTTGTGG TTGATATTGT AAAGGCCTGG GGTCAGATAA AACAGAACAA CACTGCAATA 
GTGTCTGTGA TTTTGAAAAG TGACTTAATG TATGATAGGC TCAGTGTCCT GATCGATGTC 
CTGGCTGAGG AGGCAGCAGC TACTTCTGCT GAAAAAAGTG CTACAGAATA TGCAGACAGC 
AGCAAGGCAG ATAGGAAGCC CTTCATTCCT CAAATAGACC ACATAGCCAA GTGCAAGTTG 
GAGCTGGCTA CAAAGGCCCA GAGTCTCCAG AAATCCTTGA AACTCATCAT ATTTCAAGTT 
GAACAAGCTC TGGATGAGGA AAGCAGACAG ACAATATCTG TTAAGAACTT TAGTTCAACT 
TTCTTCCTGG AAGATGACCC AGAAGATATT AACCAGACAA GCCCACGACG CCGTTCTCGT 
CGTGGCACTT TGTCTTCTAT ATCTTCTCTC AAAAGTGAGG ACCTGGACAA CCTTAACTTG 
GATCACTTAC ATTTTACACC TGAATCTATA CGCTTCAAAG AAAAATGTGT CATGAACAAC 
TACTTCGGAA TTGGACTGGA TGCTAAAATT TCTCTGGACT TCAACACCAG AAGAGATGAA 
CACCCAGGGC AATACAATAG CCGCCTTAAG AACAAGATGT GGTATGGCCT TCTGGGAACC 
AAAGAACTTT TGCAGCGCTC TTACAGGAAA CTGGAAGAAC GAGTGCATTT GGAGTGTGAT 
GGAGAAACCA TCTCCTTGCC AAACCTGCAA GGCATTGTAG TGCTCAACAT TACCAGCTAT 
GCTGGAGGTA TCAACTTCTG GGGAAGCAAC ACAGCAACCA CGGAATATGA GGCTCCTGCA 
ATCGATGATG GGAAACTGGA GGTGGTGGCA ATCTTTGGTT CTGTGCAGAT GGCAATGTCC 
CGTATCATCA ACCTGCATCA TCATCGCATT GCCCAGTGCC ATGAGGTGAT GATAACCATT 
GATGGTGAAG AAGGTATCCC AGTGCAGGTG GATGGGGAGG CCTGGATTCA GAGACCAGGC 
CTTATCAAAA TTAGATACAA GAACGCTGCC CAGATGCTGA CAAGAGATCG GGACTTTGAG 
AACTCAATGA AAATGTGGGA ATACAAGCAT ACTGAAATTC AAGCTGCCCC TCAACCCCAG 
CTGGACTTCC AGGACTCTCA AGAGAGCCTC TCTGACGAGG AGTATGCCCA GATGCAGCAC 
TTAGCTCGGC TTGCAGAAAA CCTCATCAGC AAACTTAATG ACCTGAGCAA GATCCACCAG 
CATGTGTCTG TCCTCATGGG TTCTGTGAAT GCCAGCGCTA ACATCCTGAA TGATATATTT 
TACGGCCAAG ACAGTGGCAA TGAGATGGGT GCAGCTTCCT GTATTCCCAT TGAACTGAGA 
AGTGCTGAGG ATGAGACTGC ACTACAAAGC GCCCTGGATG CCATGAATAA GGAGTTCAAA 
AAGCTATCTG AGATTGACTG GATGAATCCA ATCTTTGTTC CAGAGGAAAA ATCTTCGGAC 
ACTGACAGTA GAAGCCTCAG GCTGAAAATT AAGTTCCCCA AATTGGGAAA GAAAAAGGTA 
GAAGAGGAAC GCAAGCCTAA ATCAGGCCAG AGTGTCCAGA GTTTTATTGG CAATTTATGG 
CACCGCAGAC ATCGTGAAGA TGAAGCAGAG GGTGATGATC CTCTAACACC ATCGAGATCT 
CAACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884757.1
CDS	174..3902
Translation	MDRGAAAAQGTAPPQDGEQPAESPEPPPPWPPPPPPPAPPPAPPLLSEASPEPIPEPCPELAPGPCPEATSESATELYTEPTPEPATEPASEPAPEPATEPAPEPATEPAPEPAPEPATESAPEPTPEPALESVPEPAPELTPEVAPELAPEPTPEPVTELAPEFCPEAAPEFRPSPAPCLLQCPVDTRERGLKTSPSPSPSPSPRTPMSWSRIKKILKEGPMLKNCNSFKRWKLRYFLVQGQKLYFAHHPAFAHFETIDLSQATVAESSCRNLCHSFCVITPQRKITLAAPNRKDMEEWINIIKTIQQGEIYKIPAAENNPFLVGMHCWYSSYSHRTQHCNVCRESIPALSRDAIICEVCKVKSHRLCALRASKDCKWNTLSITDDLLLPADEVNMPHQWVEGNMPVSSQCAVCHESCGSYQRLQDFRCLWCNSTVHDDCRRRFSKECCFRSHRSSVIPPTALSDPKGDGQLVVSSDFWNLDWSSACSCPLLIFINSKSGDHQGIVFLRKFKQYLNPSQVFDLLKGGPEAGLSMFKNFARFRILVCGGDGSVSWVLSLIDAFGLHEKCQLAVIPLGTGNDLARVLGWGAFWNKSKSPLDILNRVEQASVRILDRWSVMIRETPRQTPLLKGQVEMDVPRFEAAAIQHLESAATELNKILKAKYPTEMIIATRFLCSAVEDFVVDIVKAWGQIKQNNTAIVSVILKSDLMYDRLSVLIDVLAEEAAATSAEKSATEYADSSKADRKPFIPQIDHIAKCKLELATKAQSLQKSLKLIIFQVEQALDEESRQTISVKNFSSTFFLEDDPEDINQTSPRRRSRRGTLSSISSLKSEDLDNLNLDHLHFTPESIRFKEKCVMNNYFGIGLDAKISLDFNTRRDEHPGQYNSRLKNKMWYGLLGTKELLQRSYRKLEERVHLECDGETISLPNLQGIVVLNITSYAGGINFWGSNTATTEYEAPAIDDGKLEVVAIFGSVQMAMSRIINLHHHRIAQCHEVMITIDGEEGIPVQVDGEAWIQRPGLIKIRYKNAAQMLTRDRDFENSMKMWEYKHTEIQAAPQPQLDFQDSQESLSDEEYAQMQHLARLAENLISKLNDLSKIHQHVSVLMGSVNASANILNDIFYGQDSGNEMGAASCIPIELRSAEDETALQSALDAMNKEFKKLSEIDWMNPIFVPEEKSSDTDSRSLRLKIKFPKLGKKKVEEERKPKSGQSVQSFIGNLWHRRHREDEAEGDDPLTPSRSQL

Target ORF information:

RefSeq Version	XM_017029268.2
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase kappa (DGKK), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029268.2

ORF Insert Sequence:

ATGGACCGCG GAGCTGCCGC AGCCCAGGGC ACTGCCCCGC CTCAGGATGG AGAGCAGCCC 
GCTGAGTCTC CAGAGCCTCC GCCGCCTTGG CCGCCGCCGC CACCACCACC GGCTCCGCCG 
CCGGCTCCGC CGCTGCTCTC CGAGGCTTCG CCAGAACCCA TACCAGAGCC CTGTCCAGAG 
CTTGCTCCAG GTCCCTGTCC AGAGGCGACC TCAGAATCAG CCACAGAACT GTACACAGAA 
CCGACCCCAG AACCAGCCAC AGAGCCGGCC TCAGAACCGG CCCCAGAACC TGCCACAGAG 
CCGGCCCCAG AACCTGCCAC AGAGCCGGCC CCAGAACCGG CCCCAGAACC TGCCACAGAG 
TCTGCCCCAG AGCCGACTCC AGAACCTGCC CTAGAGTCGG TCCCAGAGCC GGCCCCAGAG 
CTGACTCCAG AAGTTGCCCC AGAGCTGGCC CCAGAGCCGA CCCCAGAACC TGTGACAGAG 
CTGGCCCCAG AGTTCTGCCC TGAGGCGGCT CCAGAGTTCC GTCCAAGTCC AGCACCATGT 
CTATTGCAAT GTCCGGTGGA CACTCGAGAG AGAGGTCTAA AGACCTCGCC ATCGCCATCG 
CCATCGCCAT CGCCCAGAAC GCCAATGTCG TGGTCCAGAA TAAAGAAAAT ATTGAAGGAA 
GGACCTATGC TGAAGAACTG TAACTCTTTC AAGAGATGGA AGCTTAGATA TTTTCTGGTT 
CAAGGACAGA AGCTCTACTT TGCACACCAT CCCGCGTTTG CACACTTTGA AACGATTGAT 
CTGTCTCAAG CCACTGTGGC AGAAAGCAGC TGTAGAAACC TTTGCCACAG TTTTTGTGTT 
ATTACACCAC AACGAAAAAT CACTCTGGCT GCACCCAACC GGAAAGACAT GGAAGAATGG 
ATTAACATCA TAAAAACCAT CCAACAGGGA GAAATTTATA AGATACCTGC AGCAGAAAAC 
AACCCTTTTC TTGTTGGAAT GCATTGTTGG TACTCCAGTT ACAGCCACCG GACCCAGCAC 
TGCAATGTTT GTCGAGAGAG CATTCCTGCC TTATCTAGAG ATGCCATCAT CTGTGAAGTG 
TGCAAAGTGA AATCTCACAG ATTGTGTGCT TTGAGAGCAA GCAAAGACTG CAAGTGGAAT 
ACATTGTCTA TCACTGATGA CCTCCTTCTG CCTGCAGATG AAGTAAACAT GCCCCATCAA 
TGGGTAGAAG GAAACATGCC TGTCAGCTCT CAGTGTGCAG TGTGTCATGA GAGCTGTGGC 
AGTTATCAAA GACTTCAAGA CTTCCGCTGC CTGTGGTGTA ATTCTACGGT GCATGATGAC 
TGTAGGAGAC GGTTTTCCAA GGAATGTTGC TTCAGAAGCC ATCGCTCATC AGTCATTCCT 
CCCACTGCTC TAAGCGACCC CAAAGGCGAT GGCCAATTAG TAGTATCTTC AGACTTCTGG 
AATCTTGATT GGTCATCAGC CTGTTCATGT CCCTTGCTCA TCTTCATCAA CTCCAAAAGT 
GGCGATCATC AGGGGATCGT CTTCCTCCGA AAATTCAAGC AATACCTTAA CCCATCTCAA 
GTGTTCGACT TATTGAAGGG TGGACCTGAA GCAGGGCTGT CTATGTTCAA GAACTTTGCT 
CGCTTTCGCA TTCTGGTTTG TGGTGGAGAT GGCAGCGTGA GCTGGGTCTT ATCTCTGATT 
GATGCCTTTG GATTACATGA AAAGTGTCAG TTGGCAGTCA TCCCACTTGG AACCGGCAAT 
GATCTGGCTC GTGTTCTGGG CTGGGGTGCA TTCTGGAACA AAAGCAAGTC ACCTCTGGAC 
ATCCTCAACA GAGTGGAGCA GGCTAGTGTG AGGATCCTAG ACAGATGGAG TGTGATGATT 
CGTGAGACTC CCAGACAAAC CCCGCTGCTA AAAGGACAGG TTGAAATGGA TGTACCACGA 
TTTGAGGCTG CTGCCATCCA ACACTTAGAA TCTGCAGCCA CCGAGTTGAA CAAAATCCTG 
AAGGCCAAGT ACCCCACAGA GATGATCATC GCAACCAGAT TCTTGTGTTC AGCTGTGGAA 
GATTTTGTGG TTGATATTGT AAAGGCCTGG GGTCAGATAA AACAGAACAA CACTGCAATA 
GTGTCTGTGA TTTTGAAAAG TGACTTAATG TATGATAGGC TCAGTGTCCT GATCGATGTC 
CTGGCTGAGG AGGCAGCAGC TACTTCTGCT GAAAAAAGTG CTACAGAATA TGCAGACAGC 
AGCAAGGCAG ATAGGAAGCC CTTCATTCCT CAAATAGACC ACATAGCCAA GTGCAAGTTG 
GAGCTGGCTA CAAAGGCCCA GAGTCTCCAG AAATCCTTGA AACTCATCAT ATTTCAAGTT 
GAACAAGCTC TGGATGAGGA AAGCAGACAG ACAATATCTG TTAAGAACTT TAGTTCAACT 
TTCTTCCTGG AAGATGACCC AGAAGATATT AACCAGACAA GCCCACGACG CCGTTCTCGT 
CGTGGCACTT TGTCTTCTAT ATCTTCTCTC AAAAGTGAGG ACCTGGACAA CCTTAACTTG 
GATCACTTAC ATTTTACACC TGAATCTATA CGCTTCAAAG AAAAATGTGT CATGAACAAC 
TACTTCGGAA TTGGACTGGA TGCTAAAATT TCTCTGGACT TCAACACCAG AAGAGATGAA 
CACCCAGGGC AATACAATAG CCGCCTTAAG AACAAGATGT GGTATGGCCT TCTGGGAACC 
AAAGAACTTT TGCAGCGCTC TTACAGGAAA CTGGAAGAAC GAGTGCATTT GGAGTGTGAT 
GGAGAAACCA TCTCCTTGCC AAACCTGCAA GGCATTGTAG TGCTCAACAT TACCAGCTAT 
GCTGGAGGTA TCAACTTCTG GGGAAGCAAC ACAGCAACCA CGGAATATGA GGCTCCTGCA 
ATCGATGATG GGAAACTGGA GGTGGTGGCA ATCTTTGGTT CTGTGCAGAT GGCAATGTCC 
CGTATCATCA ACCTGCATCA TCATCGCATT GCCCAGTGCC ATGAGGTGAT GATAACCATT 
GATGGTGAAG AAGGTATCCC AGTGCAGGTG GATGGGGAGG CCTGGATTCA GAGACCAGGC 
CTTATCAAAA TTAGATACAA GAACGCTGCC CAGATGCTGA CAAGAGATCG GGACTTTGAG 
AACTCAATGA AAATGTGGGA ATACAAGCAT ACTGAAATTC AAGCTGCCCC TCAACCCCAG 
CTGGACTTCC AGGACTCTCA AGAGAGCCTC TCTGACGAGG AGTATGCCCA GATGCAGCAC 
TTAGCTCGGC TTGCAGAAAA CCTCATCAGC AAACTTAATG ACCTGAGCAA GATCCACCAG 
CATGTGTCTG TCCTCATGGG TTCTGTGAAT GCCAGCGCTA ACATCCTGAA TGATATATTT 
TACGGCCAAG ACAGTGGCAA TGAGATGGGT GCAGCTTCCT GTATTCCCAT TGAACTGAGA 
AGTGCTGAGG ATGAGACTGC ACTACAAAGC GCCCTGGATG CCATGAATAA GGAGTTCAAA 
AAGCTATCTG AGATTGACTG GATGAATCCA ATCTTTGTTC CAGAGGAAAA ATCTTCGGAC 
ACTGACAGTA GAAGCCTCAG GCTGAAAATT AAGTTCCCCA AATTGGGAAA GAAAAAGGTA 
GAAGAGGAAC GCAAGCCTAA ATCAGGCCAG AGTGTCCAGA GTTTTATTGG CAATTTATGG 
CACCGCAGAC ATCGTGAAGA TGAAGCAGAG GGTGATGATC CTCTAACACC ATCGAGATCT 
CAACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Association between diacylglycerol kinase kappa variants and hypospadias susceptibility in a Han Chinese population.
Asian journal of andrology20(1)85-89(2018 Jan-Feb)
Xie H,Lin XL,Zhang S,Yu L,Li XX,Huang YC,Lyu YQ,Chen HT,Xu J,Chen F

Fragile X Mental Retardation Protein (FMRP) controls diacylglycerol kinase activity in neurons.
Proceedings of the National Academy of Sciences of the United States of America113(26)E3619-28(2016 06)
Tabet R,Moutin E,Becker JA,Heintz D,Fouillen L,Flatter E,Kr??el W,Alunni V,Koebel P,Demb?l? D,Tassone F,Bardoni B,Mandel JL,Vitale N,Muller D,Le Merrer J,Moine H

Expression Analysis of DGKK during External Genitalia Formation.
The Journal of urology194(6)1728-36(2015 Dec)
Shen J,Liu B,Sinclair A,Cunha G,Baskin LS,Choudhry S

Diacylglycerol kinase ? (DGKK) variants and hypospadias in Han Chinese: association and meta-analysis.
BJU international116(4)634-40(2015 Oct)
Ma Q,Tang Y,Lin H,Xu M,Xu G,Fang X,Chen J,Song Z,Li Z,Shi Y,Geng H

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DGKK cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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