CD109 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CD109
Entrez Gene ID	135228
Full Name	CD109 molecule
Synonyms	CPAMD7,p180,r150
General protein information	Preferred Names CD109 molecule Names CD109 antigen 150 kDa TGF-beta-1-binding protein C3 and PZP-like alpha-2-macroglobulin domain-containing protein 7 Gov platelet alloantigens activated T-cell marker CD109 platelet-specific Gov antigen
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6q13
Summary	This gene encodes a glycosyl phosphatidylinositol (GPI)-linked glycoprotein that localizes to the surface of platelets, activated T-cells, and endothelial cells. The protein binds to and negatively regulates signalling by transforming growth factor beta (TGF-beta). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2014].
Disorder MIM:	608859

mRNA and Protein(s)

mRNA	Protein	Name
NM_001159587.2	NP_001153059.1	CD109 antigen isoform 2 preproprotein
NM_001159588.2	NP_001153060.1	CD109 antigen isoform 3 preproprotein
XM_005248659.3	XP_005248716.1	CD109 antigen isoform X1
XM_017010283.1	XP_016865772.1	CD109 antigen isoform X3
NM_133493.4	NP_598000.2	CD109 antigen isoform 1 preproprotein
NM_133493.5	NP_598000.2	CD109 antigen isoform 1 preproprotein
XM_011535473.1	XP_011533775.1	CD109 antigen isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	CD109	NP_598000.2
Pan troglodytes (chimpanzee)	CD109	XP_518588.2
	CD109	XP_001112898.2
Canis lupus familiaris (dog)	CD109	XP_532205.3
Mus musculus (house mouse)	Cd109	NP_694738.1
Gallus gallus (chicken)	CD109	XP_004940486.1
Caenorhabditis elegans (roundworm)	tep-1	NP_001021867.1
Xenopus tropicalis (tropical clawed frog)	LOC100487408	XP_002937196.2
Rattus norvegicus (Norway rat)	Cd109	XP_003750594.1
Danio rerio (zebrafish)	cd109	XP_697512.6
Bos taurus (cattle)	CD109	XP_002690068.2
Anopheles gambiae (African malaria mosquito)	TEP15	XP_317088.3
Anopheles gambiae (African malaria mosquito)	TEP2	XP_555086.4
Xenopus tropicalis (tropical clawed frog)	LOC100486953	XP_002937194.2
Xenopus tropicalis (tropical clawed frog)	LOC101734998	XP_004919269.1
Drosophila melanogaster (fruit fly)	Tep3	NP_523507.2

Gene Ontology
Bibliography

Related articles in PubMed

Significance of perivascular tumour cells defined by CD109 expression in progression of glioma.
Shiraki Y, Mii S, Enomoto A, Momota H, Han YP, Kato T, Ushida K, Kato A, Asai N, Murakumo Y, Aoki K, Suzuki H, Ohka F, Wakabayashi T, Todo T, Ogawa S, Natsume A, Takahashi M
The Journal of pathology243(4)468-480(2017 Dec)

CD109, a negative regulator of TGF-? signaling, is a putative risk marker in diffuse large B-cell lymphoma.
Yokoyama M, Ichinoe M, Okina S, Sakurai Y, Nakada N, Yanagisawa N, Jiang SX, Numata Y, Umezawa A, Miyazaki K, Higashihara M, Murakumo Y
International journal of hematology105(5)614-622(2017 May)

CD109 Mediates Cell Survival in Hepatocellular Carcinoma Cells.
Zong G, Xu Z, Zhang S, Shen Y, Qiu H, Zhu G, He S, Tao T, Chen X
Digestive diseases and sciences61(8)2303-14(2016 08)

Reduced expression of CD109 in tumor-associated endothelial cells promotes tumor progression by paracrine interleukin-8 in hepatocellular carcinoma.
Ye BG, Sun HC, Zhu XD, Chai ZT, Zhang YY, Ao JY, Cai H, Ma DN, Wang CH, Qin CD, Gao DM, Tang ZY
Oncotarget7(20)29333-45(2016 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

High expression of CD109 in brain tumor stem cells is involved in glioma progression.
Title: Significance of perivascular tumour cells defined by CD109 expression in progression of glioma.

CD109 is a putative biomarker for identifying a high-risk group among DLBCL patients.
Title: CD109, a negative regulator of TGF-? signaling, is a putative risk marker in diffuse large B-cell lymphoma.

The CD109 protein was up-regulated in hepatocellular carcinoma tissue compared with adjacent noncancerous tissue. CD109 shRNA knockdown delayed the G1-S phase transition, abrogated cell proliferation, and increased cell apoptosis. Furthermore, CD109 impaired TGF-beta/Smad signaling through control of p-smad2.
Title: CD109 Mediates Cell Survival in Hepatocellular Carcinoma Cells.

The most common HPA genotypes among Saudis were HPA-1 a + b- (75%), HPA-2 a + b- (62%), HPA-3 a + b- (51.5%), HPA-4 a + b- (99%), HPA-5 a + b- (76.5%), HPA-6 a + b- (100%) and HPA-15 a + b + (50%). The prevalent allele among the HPA systems was (a), except in the HPA-15 system where the (b) allele was found in 52% of the subjects.
Title: Gene frequency of human platelet alloantigens-1 to -6 and -15 in Saudi blood donors.

CD109 knockdown upregulated IL-8 expression through activation of TGF-beta/Akt/NF-kappaB pathway in human umbilical vein endothelial cells
Title: Reduced expression of CD109 in tumor-associated endothelial cells promotes tumor progression by paracrine interleukin-8 in hepatocellular carcinoma.

The following CD109 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CD109 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17039	NM_133493.5 Latest version!	Homo sapiens CD109 molecule (CD109), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$519.50-$727.30 $1039.00
OHu75773	XM_017010283.1 Latest version!	Homo sapiens CD109 molecule (CD109), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu17040	NM_001159587.2 Latest version!	Homo sapiens CD109 molecule (CD109), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu17041	NM_001159588.2 Latest version!	Homo sapiens CD109 molecule (CD109), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu17039	XM_005248659.3 Latest version!	Homo sapiens CD109 molecule (CD109), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu75772	XM_011535473.1 Latest version!	Homo sapiens CD109 molecule (CD109), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OHu17039	NM_133493.4	Homo sapiens CD109 molecule (CD109), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CD109 ( NM_133493.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_598000.2 Homo sapiens CD109 molecule (CD109), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu17039
Clone ID Related Accession (Same CDS sequence)	XM_005248659.3 , NM_133493.4 , NM_133493.5
Accession Version	NM_133493.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	CD109 antigen isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF410459.1, AK095888.1, AL834478.1, BX646244.1, BX107999.1, CB529476.1, BM722643.1, AK130104.1 and CB853216.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_133493.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF410459.1, EF553520.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000287097.6/ ENSP00000287097.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCAGGGCC CACCGCTCCT GACCGCCGCC CACCTCCTCT GCGTGTGCAC CGCCGCGCTG 
GCCGTGGCTC CCGGGCCTCG GTTTCTGGTG ACAGCCCCAG GGATCATCAG GCCCGGAGGA 
AATGTGACTA TTGGGGTGGA GCTTCTGGAA CACTGCCCTT CACAGGTGAC TGTGAAGGCG 
GAGCTGCTCA AGACAGCATC AAACCTCACT GTCTCTGTCC TGGAAGCAGA AGGAGTCTTT 
GAAAAAGGCT CTTTTAAGAC ACTTACTCTT CCATCACTAC CTCTGAACAG TGCAGATGAG 
ATTTATGAGC TACGTGTAAC CGGACGTACC CAGGATGAGA TTTTATTCTC TAATAGTACC 
CGCTTATCAT TTGAGACCAA GAGAATATCT GTCTTCATTC AAACAGACAA GGCCTTATAC 
AAGCCAAAGC AAGAAGTGAA GTTTCGCATT GTTACACTCT TCTCAGATTT TAAGCCTTAC 
AAAACCTCTT TAAACATTCT CATTAAGGAC CCCAAATCAA ATTTGATCCA ACAGTGGTTG 
TCACAACAAA GTGATCTTGG AGTCATTTCC AAAACTTTTC AGCTATCTTC CCATCCAATA 
CTTGGTGACT GGTCTATTCA AGTTCAAGTG AATGACCAGA CATACTATCA ATCATTTCAG 
GTTTCAGAAT ATGTATTACC AAAATTTGAA GTGACTTTGC AGACACCATT ATATTGTTCT 
ATGAATTCTA AGCATTTAAA TGGTACCATC ACGGCAAAGT ATACATATGG GAAGCCAGTG 
AAAGGAGACG TAACGCTTAC ATTTTTACCT TTATCCTTTT GGGGAAAGAA GAAAAATATT 
ACAAAAACAT TTAAGATAAA TGGATCTGCA AACTTCTCTT TTAATGATGA AGAGATGAAA 
AATGTAATGG ATTCTTCAAA TGGACTTTCT GAATACCTGG ATCTATCTTC CCCTGGACCA 
GTAGAAATTT TAACCACAGT GACAGAATCA GTTACAGGTA TTTCAAGAAA TGTAAGCACT 
AATGTGTTCT TCAAGCAACA TGATTACATC ATTGAGTTTT TTGATTATAC TACTGTCTTG 
AAGCCATCTC TCAACTTCAC AGCCACTGTG AAGGTAACTC GTGCTGATGG CAACCAACTG 
ACTCTTGAAG AAAGAAGAAA TAATGTAGTC ATAACAGTGA CACAGAGAAA CTATACTGAG 
TACTGGAGCG GATCTAACAG TGGAAATCAG AAAATGGAAG CTGTTCAGAA AATAAATTAT 
ACTGTCCCCC AAAGTGGAAC TTTTAAGATT GAATTCCCAA TCCTGGAGGA TTCCAGTGAG 
CTACAGTTGA AGGCCTATTT CCTTGGTAGT AAAAGTAGCA TGGCAGTTCA TAGTCTGTTT 
AAGTCTCCTA GTAAGACATA CATCCAACTA AAAACAAGAG ATGAAAATAT AAAGGTGGGA 
TCGCCTTTTG AGTTGGTGGT TAGTGGCAAC AAACGATTGA AGGAGTTAAG CTATATGGTA 
GTATCCAGGG GACAGTTGGT GGCTGTAGGA AAACAAAATT CAACAATGTT CTCTTTAACA 
CCAGAAAATT CTTGGACTCC AAAAGCCTGT GTAATTGTGT ATTATATTGA AGATGATGGG 
GAAATTATAA GTGATGTTCT AAAAATTCCT GTTCAGCTTG TTTTTAAAAA TAAGATAAAG 
CTATATTGGA GTAAAGTGAA AGCTGAACCA TCTGAGAAAG TCTCTCTTAG GATCTCTGTG 
ACACAGCCTG ACTCCATAGT TGGGATTGTA GCTGTTGACA AAAGTGTGAA TCTGATGAAT 
GCCTCTAATG ATATTACAAT GGAAAATGTG GTCCATGAGT TGGAACTTTA TAACACAGGA 
TATTATTTAG GCATGTTCAT GAATTCTTTT GCAGTCTTTC AGGAATGTGG ACTCTGGGTA 
TTGACAGATG CAAACCTCAC GAAGGATTAT ATTGATGGTG TTTATGACAA TGCAGAATAT 
GCTGAGAGGT TTATGGAGGA AAATGAAGGA CATATTGTAG ATATTCATGA CTTTTCTTTG 
GGTAGCAGTC CACATGTCCG AAAGCATTTT CCAGAGACTT GGATTTGGCT AGACACCAAC 
ATGGGTTACA GGATTTACCA AGAATTTGAA GTAACTGTAC CTGATTCTAT CACTTCTTGG 
GTGGCTACTG GTTTTGTGAT CTCTGAGGAC CTGGGTCTTG GACTAACAAC TACTCCAGTG 
GAGCTCCAAG CCTTCCAACC ATTTTTCATT TTTTTGAATC TTCCCTACTC TGTTATCAGA 
GGTGAAGAAT TTGCTTTGGA AATAACTATA TTCAATTATT TGAAAGATGC CACTGAGGTT 
AAGGTAATCA TTGAGAAAAG TGACAAATTT GATATTCTAA TGACTTCAAA TGAAATAAAT 
GCCACAGGCC ACCAGCAGAC CCTTCTGGTT CCCAGTGAGG ATGGGGCAAC TGTTCTTTTT 
CCCATCAGGC CAACACATCT GGGAGAAATT CCTATCACAG TCACAGCTCT TTCACCCACT 
GCTTCTGATG CTGTCACCCA GATGATTTTA GTAAAGGCTG AAGGAATAGA AAAATCATAT 
TCACAATCCA TCTTATTAGA CTTGACTGAC AATAGGCTAC AGAGTACCCT GAAAACTTTG 
AGTTTCTCAT TTCCTCCTAA TACAGTGACT GGCAGTGAAA GAGTTCAGAT CACTGCAATT 
GGAGATGTTC TTGGTCCTTC CATCAATGGC TTAGCCTCAT TGATTCGGAT GCCTTATGGC 
TGTGGTGAAC AGAACATGAT AAATTTTGCT CCAAATATTT ACATTTTGGA TTATCTGACT 
AAAAAGAAAC AACTGACAGA TAATTTGAAA GAAAAAGCTC TTTCATTTAT GAGGCAAGGT 
TACCAGAGAG AACTTCTCTA TCAGAGGGAA GATGGCTCTT TCAGTGCTTT TGGGAATTAT 
GACCCTTCTG GGAGCACTTG GTTGTCAGCT TTTGTTTTAA GATGTTTCCT TGAAGCCGAT 
CCTTACATAG ATATTGATCA GAATGTGTTA CACAGAACAT ACACTTGGCT TAAAGGACAT 
CAGAAATCCA ACGGTGAATT TTGGGATCCA GGAAGAGTGA TTCATAGTGA GCTTCAAGGT 
GGCAATAAAA GTCCAGTAAC ACTTACAGCC TATATTGTAA CTTCTCTCCT GGGATATAGA 
AAGTATCAGC CTAACATTGA TGTGCAAGAG TCTATCCATT TTTTGGAGTC TGAATTCAGT 
AGAGGAATTT CAGACAATTA TACTCTAGCC CTTATAACTT ATGCATTGTC ATCAGTGGGG 
AGTCCTAAAG CGAAGGAAGC TTTGAATATG CTGACTTGGA GAGCAGAACA AGAAGGTGGC 
ATGCAATTCT GGGTGTCATC AGAGTCCAAA CTTTCTGACT CCTGGCAGCC ACGCTCCCTG 
GATATTGAAG TTGCAGCCTA TGCACTGCTC TCACACTTCT TACAATTTCA GACTTCTGAG 
GGAATCCCAA TTATGAGGTG GCTAAGCAGG CAAAGAAATA GCTTGGGTGG TTTTGCATCT 
ACTCAGGATA CCACTGTGGC TTTAAAGGCT CTGTCTGAAT TTGCAGCCCT AATGAATACA 
GAAAGGACAA ATATCCAAGT GACCGTGACG GGGCCTAGCT CACCAAGTCC TGTAAAGTTT 
CTGATTGACA CACACAACCG CTTACTCCTT CAGACAGCAG AGCTTGCTGT GGTACAGCCA 
ACGGCAGTTA ATATTTCCGC AAATGGTTTT GGATTTGCTA TTTGTCAGCT CAATGTTGTA 
TATAATGTGA AGGCTTCTGG GTCTTCTAGA AGACGAAGAT CTATCCAAAA TCAAGAAGCC 
TTTGATTTAG ATGTTGCTGT AAAAGAAAAT AAAGATGATC TCAATCATGT GGATTTGAAT 
GTGTGTACAA GCTTTTCGGG CCCGGGTAGG AGTGGCATGG CTCTTATGGA AGTTAACCTA 
TTAAGTGGCT TTATGGTGCC TTCAGAAGCA ATTTCTCTGA GCGAGACAGT GAAGAAAGTG 
GAATATGATC ATGGAAAACT CAACCTCTAT TTAGATTCTG TAAATGAAAC CCAGTTTTGT 
GTTAATATTC CTGCTGTGAG AAACTTTAAA GTTTCAAATA CCCAAGATGC TTCAGTGTCC 
ATAGTGGATT ACTATGAGCC AAGGAGACAG GCGGTGAGAA GTTACAACTC TGAAGTGAAG 
CTGTCCTCCT GTGACCTTTG CAGTGATGTC CAGGGCTGCC GTCCTTGTGA GGATGGAGCT 
TCAGGCTCCC ATCATCACTC TTCAGTCATT TTTATTTTCT GTTTCAAGCT TCTGTACTTT 
ATGGAACTTT GGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598000.2
CDS	14..4351
Translation	MQGPPLLTAAHLLCVCTAALAVAPGPRFLVTAPGIIRPGGNVTIGVELLEHCPSQVTVKAELLKTASNLTVSVLEAEGVFEKGSFKTLTLPSLPLNSADEIYELRVTGRTQDEILFSNSTRLSFETKRISVFIQTDKALYKPKQEVKFRIVTLFSDFKPYKTSLNILIKDPKSNLIQQWLSQQSDLGVISKTFQLSSHPILGDWSIQVQVNDQTYYQSFQVSEYVLPKFEVTLQTPLYCSMNSKHLNGTITAKYTYGKPVKGDVTLTFLPLSFWGKKKNITKTFKINGSANFSFNDEEMKNVMDSSNGLSEYLDLSSPGPVEILTTVTESVTGISRNVSTNVFFKQHDYIIEFFDYTTVLKPSLNFTATVKVTRADGNQLTLEERRNNVVITVTQRNYTEYWSGSNSGNQKMEAVQKINYTVPQSGTFKIEFPILEDSSELQLKAYFLGSKSSMAVHSLFKSPSKTYIQLKTRDENIKVGSPFELVVSGNKRLKELSYMVVSRGQLVAVGKQNSTMFSLTPENSWTPKACVIVYYIEDDGEIISDVLKIPVQLVFKNKIKLYWSKVKAEPSEKVSLRISVTQPDSIVGIVAVDKSVNLMNASNDITMENVVHELELYNTGYYLGMFMNSFAVFQECGLWVLTDANLTKDYIDGVYDNAEYAERFMEENEGHIVDIHDFSLGSSPHVRKHFPETWIWLDTNMGYRIYQEFEVTVPDSITSWVATGFVISEDLGLGLTTTPVELQAFQPFFIFLNLPYSVIRGEEFALEITIFNYLKDATEVKVIIEKSDKFDILMTSNEINATGHQQTLLVPSEDGATVLFPIRPTHLGEIPITVTALSPTASDAVTQMILVKAEGIEKSYSQSILLDLTDNRLQSTLKTLSFSFPPNTVTGSERVQITAIGDVLGPSINGLASLIRMPYGCGEQNMINFAPNIYILDYLTKKKQLTDNLKEKALSFMRQGYQRELLYQREDGSFSAFGNYDPSGSTWLSAFVLRCFLEADPYIDIDQNVLHRTYTWLKGHQKSNGEFWDPGRVIHSELQGGNKSPVTLTAYIVTSLLGYRKYQPNIDVQESIHFLESEFSRGISDNYTLALITYALSSVGSPKAKEALNMLTWRAEQEGGMQFWVSSESKLSDSWQPRSLDIEVAAYALLSHFLQFQTSEGIPIMRWLSRQRNSLGGFASTQDTTVALKALSEFAALMNTERTNIQVTVTGPSSPSPVKFLIDTHNRLLLQTAELAVVQPTAVNISANGFGFAICQLNVVYNVKASGSSRRRRSIQNQEAFDLDVAVKENKDDLNHVDLNVCTSFSGPGRSGMALMEVNLLSGFMVPSEAISLSETVKKVEYDHGKLNLYLDSVNETQFCVNIPAVRNFKVSNTQDASVSIVDYYEPRRQAVRSYNSEVKLSSCDLCSDVQGCRPCEDGASGSHHHSSVIFIFCFKLLYFMELWL

Target ORF information:

RefSeq Version	NM_133493.5
Organism	Homo sapiens(human)
Definition	Homo sapiens CD109 molecule (CD109), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133493.5

ORF Insert Sequence:

ATGCAGGGCC CACCGCTCCT GACCGCCGCC CACCTCCTCT GCGTGTGCAC CGCCGCGCTG 
GCCGTGGCTC CCGGGCCTCG GTTTCTGGTG ACAGCCCCAG GGATCATCAG GCCCGGAGGA 
AATGTGACTA TTGGGGTGGA GCTTCTGGAA CACTGCCCTT CACAGGTGAC TGTGAAGGCG 
GAGCTGCTCA AGACAGCATC AAACCTCACT GTCTCTGTCC TGGAAGCAGA AGGAGTCTTT 
GAAAAAGGCT CTTTTAAGAC ACTTACTCTT CCATCACTAC CTCTGAACAG TGCAGATGAG 
ATTTATGAGC TACGTGTAAC CGGACGTACC CAGGATGAGA TTTTATTCTC TAATAGTACC 
CGCTTATCAT TTGAGACCAA GAGAATATCT GTCTTCATTC AAACAGACAA GGCCTTATAC 
AAGCCAAAGC AAGAAGTGAA GTTTCGCATT GTTACACTCT TCTCAGATTT TAAGCCTTAC 
AAAACCTCTT TAAACATTCT CATTAAGGAC CCCAAATCAA ATTTGATCCA ACAGTGGTTG 
TCACAACAAA GTGATCTTGG AGTCATTTCC AAAACTTTTC AGCTATCTTC CCATCCAATA 
CTTGGTGACT GGTCTATTCA AGTTCAAGTG AATGACCAGA CATACTATCA ATCATTTCAG 
GTTTCAGAAT ATGTATTACC AAAATTTGAA GTGACTTTGC AGACACCATT ATATTGTTCT 
ATGAATTCTA AGCATTTAAA TGGTACCATC ACGGCAAAGT ATACATATGG GAAGCCAGTG 
AAAGGAGACG TAACGCTTAC ATTTTTACCT TTATCCTTTT GGGGAAAGAA GAAAAATATT 
ACAAAAACAT TTAAGATAAA TGGATCTGCA AACTTCTCTT TTAATGATGA AGAGATGAAA 
AATGTAATGG ATTCTTCAAA TGGACTTTCT GAATACCTGG ATCTATCTTC CCCTGGACCA 
GTAGAAATTT TAACCACAGT GACAGAATCA GTTACAGGTA TTTCAAGAAA TGTAAGCACT 
AATGTGTTCT TCAAGCAACA TGATTACATC ATTGAGTTTT TTGATTATAC TACTGTCTTG 
AAGCCATCTC TCAACTTCAC AGCCACTGTG AAGGTAACTC GTGCTGATGG CAACCAACTG 
ACTCTTGAAG AAAGAAGAAA TAATGTAGTC ATAACAGTGA CACAGAGAAA CTATACTGAG 
TACTGGAGCG GATCTAACAG TGGAAATCAG AAAATGGAAG CTGTTCAGAA AATAAATTAT 
ACTGTCCCCC AAAGTGGAAC TTTTAAGATT GAATTCCCAA TCCTGGAGGA TTCCAGTGAG 
CTACAGTTGA AGGCCTATTT CCTTGGTAGT AAAAGTAGCA TGGCAGTTCA TAGTCTGTTT 
AAGTCTCCTA GTAAGACATA CATCCAACTA AAAACAAGAG ATGAAAATAT AAAGGTGGGA 
TCGCCTTTTG AGTTGGTGGT TAGTGGCAAC AAACGATTGA AGGAGTTAAG CTATATGGTA 
GTATCCAGGG GACAGTTGGT GGCTGTAGGA AAACAAAATT CAACAATGTT CTCTTTAACA 
CCAGAAAATT CTTGGACTCC AAAAGCCTGT GTAATTGTGT ATTATATTGA AGATGATGGG 
GAAATTATAA GTGATGTTCT AAAAATTCCT GTTCAGCTTG TTTTTAAAAA TAAGATAAAG 
CTATATTGGA GTAAAGTGAA AGCTGAACCA TCTGAGAAAG TCTCTCTTAG GATCTCTGTG 
ACACAGCCTG ACTCCATAGT TGGGATTGTA GCTGTTGACA AAAGTGTGAA TCTGATGAAT 
GCCTCTAATG ATATTACAAT GGAAAATGTG GTCCATGAGT TGGAACTTTA TAACACAGGA 
TATTATTTAG GCATGTTCAT GAATTCTTTT GCAGTCTTTC AGGAATGTGG ACTCTGGGTA 
TTGACAGATG CAAACCTCAC GAAGGATTAT ATTGATGGTG TTTATGACAA TGCAGAATAT 
GCTGAGAGGT TTATGGAGGA AAATGAAGGA CATATTGTAG ATATTCATGA CTTTTCTTTG 
GGTAGCAGTC CACATGTCCG AAAGCATTTT CCAGAGACTT GGATTTGGCT AGACACCAAC 
ATGGGTTACA GGATTTACCA AGAATTTGAA GTAACTGTAC CTGATTCTAT CACTTCTTGG 
GTGGCTACTG GTTTTGTGAT CTCTGAGGAC CTGGGTCTTG GACTAACAAC TACTCCAGTG 
GAGCTCCAAG CCTTCCAACC ATTTTTCATT TTTTTGAATC TTCCCTACTC TGTTATCAGA 
GGTGAAGAAT TTGCTTTGGA AATAACTATA TTCAATTATT TGAAAGATGC CACTGAGGTT 
AAGGTAATCA TTGAGAAAAG TGACAAATTT GATATTCTAA TGACTTCAAA TGAAATAAAT 
GCCACAGGCC ACCAGCAGAC CCTTCTGGTT CCCAGTGAGG ATGGGGCAAC TGTTCTTTTT 
CCCATCAGGC CAACACATCT GGGAGAAATT CCTATCACAG TCACAGCTCT TTCACCCACT 
GCTTCTGATG CTGTCACCCA GATGATTTTA GTAAAGGCTG AAGGAATAGA AAAATCATAT 
TCACAATCCA TCTTATTAGA CTTGACTGAC AATAGGCTAC AGAGTACCCT GAAAACTTTG 
AGTTTCTCAT TTCCTCCTAA TACAGTGACT GGCAGTGAAA GAGTTCAGAT CACTGCAATT 
GGAGATGTTC TTGGTCCTTC CATCAATGGC TTAGCCTCAT TGATTCGGAT GCCTTATGGC 
TGTGGTGAAC AGAACATGAT AAATTTTGCT CCAAATATTT ACATTTTGGA TTATCTGACT 
AAAAAGAAAC AACTGACAGA TAATTTGAAA GAAAAAGCTC TTTCATTTAT GAGGCAAGGT 
TACCAGAGAG AACTTCTCTA TCAGAGGGAA GATGGCTCTT TCAGTGCTTT TGGGAATTAT 
GACCCTTCTG GGAGCACTTG GTTGTCAGCT TTTGTTTTAA GATGTTTCCT TGAAGCCGAT 
CCTTACATAG ATATTGATCA GAATGTGTTA CACAGAACAT ACACTTGGCT TAAAGGACAT 
CAGAAATCCA ACGGTGAATT TTGGGATCCA GGAAGAGTGA TTCATAGTGA GCTTCAAGGT 
GGCAATAAAA GTCCAGTAAC ACTTACAGCC TATATTGTAA CTTCTCTCCT GGGATATAGA 
AAGTATCAGC CTAACATTGA TGTGCAAGAG TCTATCCATT TTTTGGAGTC TGAATTCAGT 
AGAGGAATTT CAGACAATTA TACTCTAGCC CTTATAACTT ATGCATTGTC ATCAGTGGGG 
AGTCCTAAAG CGAAGGAAGC TTTGAATATG CTGACTTGGA GAGCAGAACA AGAAGGTGGC 
ATGCAATTCT GGGTGTCATC AGAGTCCAAA CTTTCTGACT CCTGGCAGCC ACGCTCCCTG 
GATATTGAAG TTGCAGCCTA TGCACTGCTC TCACACTTCT TACAATTTCA GACTTCTGAG 
GGAATCCCAA TTATGAGGTG GCTAAGCAGG CAAAGAAATA GCTTGGGTGG TTTTGCATCT 
ACTCAGGATA CCACTGTGGC TTTAAAGGCT CTGTCTGAAT TTGCAGCCCT AATGAATACA 
GAAAGGACAA ATATCCAAGT GACCGTGACG GGGCCTAGCT CACCAAGTCC TGTAAAGTTT 
CTGATTGACA CACACAACCG CTTACTCCTT CAGACAGCAG AGCTTGCTGT GGTACAGCCA 
ACGGCAGTTA ATATTTCCGC AAATGGTTTT GGATTTGCTA TTTGTCAGCT CAATGTTGTA 
TATAATGTGA AGGCTTCTGG GTCTTCTAGA AGACGAAGAT CTATCCAAAA TCAAGAAGCC 
TTTGATTTAG ATGTTGCTGT AAAAGAAAAT AAAGATGATC TCAATCATGT GGATTTGAAT 
GTGTGTACAA GCTTTTCGGG CCCGGGTAGG AGTGGCATGG CTCTTATGGA AGTTAACCTA 
TTAAGTGGCT TTATGGTGCC TTCAGAAGCA ATTTCTCTGA GCGAGACAGT GAAGAAAGTG 
GAATATGATC ATGGAAAACT CAACCTCTAT TTAGATTCTG TAAATGAAAC CCAGTTTTGT 
GTTAATATTC CTGCTGTGAG AAACTTTAAA GTTTCAAATA CCCAAGATGC TTCAGTGTCC 
ATAGTGGATT ACTATGAGCC AAGGAGACAG GCGGTGAGAA GTTACAACTC TGAAGTGAAG 
CTGTCCTCCT GTGACCTTTG CAGTGATGTC CAGGGCTGCC GTCCTTGTGA GGATGGAGCT 
TCAGGCTCCC ATCATCACTC TTCAGTCATT TTTATTTTCT GTTTCAAGCT TCTGTACTTT 
ATGGAACTTT GGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CD109 ( XM_017010283.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865772.1 Homo sapiens CD109 molecule (CD109), transcript variant X3, mRNA.

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CloneID	OHu75773
Clone ID Related Accession (Same CDS sequence)	XM_017010283.1
Accession Version	XM_017010283.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	CD109 antigen isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCTA AGCATTTAAA TGGTACCATC ACGGCAAAGT ATACATATGG GAAGCCAGTG 
AAAGGAGACG TAACGCTTAC ATTTTTACCT TTATCCTTTT GGGGAAAGAA GAAAAATATT 
ACAAAAACAT TTAAGATAAA TGGATCTGCA AACTTCTCTT TTAATGATGA AGAGATGAAA 
AATGTAATGG ATTCTTCAAA TGGACTTTCT GAATACCTGG ATCTATCTTC CCCTGGACCA 
GTAGAAATTT TAACCACAGT GACAGAATCA GTTACAGGTA TTTCAAGAAA TGTAAGCACT 
AATGTGTTCT TCAAGCAACA TGATTACATC ATTGAGTTTT TTGATTATAC TACTGTCTTG 
AAGCCATCTC TCAACTTCAC AGCCACTGTG AAGGTAACTC GTGCTGATGG CAACCAACTG 
ACTCTTGAAG AAAGAAGAAA TAATGTAGTC ATAACAGTGA CACAGAGAAA CTATACTGAG 
TACTGGAGCG GATCTAACAG TGGAAATCAG AAAATGGAAG CTGTTCAGAA AATAAATTAT 
ACTGTCCCCC AAAGTGGAAC TTTTAAGATT GAATTCCCAA TCCTGGAGGA TTCCAGTGAG 
CTACAGTTGA AGGCCTATTT CCTTGGTAGT AAAAGTAGCA TGGCAGTTCA TAGTCTGTTT 
AAGTCTCCTA GTAAGACATA CATCCAACTA AAAACAAGAG ATGAAAATAT AAAGGTGGGA 
TCGCCTTTTG AGTTGGTGGT TAGTGGCAAC AAACGATTGA AGGAGTTAAG CTATATGGTA 
GTATCCAGGG GACAGTTGGT GGCTGTAGGA AAACAAAATT CAACAATGTT CTCTTTAACA 
CCAGAAAATT CTTGGACTCC AAAAGCCTGT GTAATTGTGT ATTATATTGA AGATGATGGG 
GAAATTATAA GTGATGTTCT AAAAATTCCT GTTCAGCTTG TTTTTAAAAA TAAGATAAAG 
CTATATTGGA GTAAAGTGAA AGCTGAACCA TCTGAGAAAG TCTCTCTTAG GATCTCTGTG 
ACACAGCCTG ACTCCATAGT TGGGATTGTA GCTGTTGACA AAAGTGTGAA TCTGATGAAT 
GCCTCTAATG ATATTACAAT GGAAAATGTG GTCCATGAGT TGGAACTTTA TAACACAGGA 
TATTATTTAG GCATGTTCAT GAATTCTTTT GCAGTCTTTC AGGAATGTGG ACTCTGGGTA 
TTGACAGATG CAAACCTCAC GAAGGATTAT ATTGATGGTG TTTATGACAA TGCAGAATAT 
GCTGAGAGGT TTATGGAGGA AAATGAAGGA CATATTGTAG ATATTCATGA CTTTTCTTTG 
GGTAGCAGTC CACATGTCCG AAAGCATTTT CCAGAGACTT GGATTTGGCT AGACACCAAC 
ATGGGTTACA GGATTTACCA AGAATTTGAA GTAACTGTAC CTGATTCTAT CACTTCTTGG 
GTGGCTACTG GTTTTGTGAT CTCTGAGGAC CTGGGTCTTG GACTAACAAC TACTCCAGTG 
GAGCTCCAAG CCTTCCAACC ATTTTTCATT TTTTTGAATC TTCCCTACTC TGTTATCAGA 
GGTGAAGAAT TTGCTTTGGA AATAACTATA TTCAATTATT TGAAAGATGC CACTGAGGTT 
AAGGTAATCA TTGAGAAAAG TGACAAATTT GATATTCTAA TGACTTCAAA TGAAATAAAT 
GCCACAGGCC ACCAGCAGAC CCTTCTGGTT CCCAGTGAGG ATGGGGCAAC TGTTCTTTTT 
CCCATCAGGC CAACACATCT GGGAGAAATT CCTATCACAG TCACAGCTCT TTCACCCACT 
GCTTCTGATG CTGTCACCCA GATGATTTTA GTAAAGGCTG AAGGAATAGA AAAATCATAT 
TCACAATCCA TCTTATTAGA CTTGACTGAC AATAGGCTAC AGAGTACCCT GAAAACTTTG 
AGTTTCTCAT TTCCTCCTAA TACAGTGACT GGCAGTGAAA GAGTTCAGAT CACTGCAATT 
GGAGATGTTC TTGGTCCTTC CATCAATGGC TTAGCCTCAT TGATTCGGAT GCCTTATGGC 
TGTGGTGAAC AGAACATGAT AAATTTTGCT CCAAATATTT ACATTTTGGA TTATCTGACT 
AAAAAGAAAC AACTGACAGA TAATTTGAAA GAAAAAGCTC TTTCATTTAT GAGGCAAGGT 
TACCAGAGAG AACTTCTCTA TCAGAGGGAA GATGGCTCTT TCAGTGCTTT TGGGAATTAT 
GACCCTTCTG GGAGCACTTG GTTGTCAGCT TTTGTTTTAA GATGTTTCCT TGAAGCCGAT 
CCTTACATAG ATATTGATCA GAATGTGTTA CACAGAACAT ACACTTGGCT TAAAGGACAT 
CAGAAATCCA ACGGTGAATT TTGGGATCCA GGAAGAGTGA TTCATAGTGA GCTTCAAGGT 
GGCAATAAAA GTCCAGTAAC ACTTACAGCC TATATTGTAA CTTCTCTCCT GGGATATAGA 
AAGTATCAGC CTAACATTGA TGTGCAAGAG TCTATCCATT TTTTGGAGTC TGAATTCAGT 
AGAGGAATTT CAGACAATTA TACTCTAGCC CTTATAACTT ATGCATTGTC ATCAGTGGGG 
AGTCCTAAAG CGAAGGAAGC TTTGAATATG CTGACTTGGA GAGCAGAACA AGAAGGTGGC 
ATGCAATTCT GGGTGTCATC AGAGTCCAAA CTTTCTGACT CCTGGCAGCC ACGCTCCCTG 
GATATTGAAG TTGCAGCCTA TGCACTGCTC TCACACTTCT TACAATTTCA GACTTCTGAG 
GGAATCCCAA TTATGAGGTG GCTAAGCAGG CAAAGAAATA GCTTGGGTGG TTTTGCATCT 
ACTCAGGATA CCACTGTGGC TTTAAAGGCT CTGTCTGAAT TTGCAGCCCT AATGAATACA 
GAAAGGACAA ATATCCAAGT GACCGTGACG GGGCCTAGCT CACCAAGTCC TGTAAAGTTT 
CTGATTGACA CACACAACCG CTTACTCCTT CAGACAGCAG AGCTTGCTGT GGTACAGCCA 
ACGGCAGTTA ATATTTCCGC AAATGGTTTT GGATTTGCTA TTTGTCAGCT CAATGTTGTA 
TATAATGTGA AGGCTTCTGG GTCTTCTAGA AGACGAAGAT CTATCCAAAA TCAAGAAGCC 
TTTGATTTAG ATGTTGCTGT AAAAGAAAAT AAAGATGATC TCAATCATGT GGATTTGAAT 
GTGTGTACAA GCTTTTCGGG CCCGGGTAGG AGTGGCATGG CTCTTATGGA AGTTAACCTA 
TTAAGTGGCT TTATGGTGCC TTCAGAAGCA ATTTCTCTGA GCGAGACAGT GAAGAAAGTG 
GAATATGATC ATGGAAAACT CAACCTCTAT TTAGATTCTG TAAATGAAAC CCAGTTTTGT 
GTTAATATTC CTGCTGTGAG AAACTTTAAA GTTTCAAATA CCCAAGATGC TTCAGTGTCC 
ATAGTGGATT ACTATGAGCC AAGGAGACAG GCGGTGAGAA GTTACAACTC TGAAGTGAAG 
CTGTCCTCCT GTGACCTTTG CAGTGATGTC CAGGGCTGCC GTCCTTGTGA GGATGGAGCT 
TCAGGCTCCC ATCATCACTC TTCAGTCATT TTTATTTTCT GTTTCAAGCT TCTGTACTTT 
ATGGAACTTT GGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865772.1
CDS	464..4081
Translation	MNSKHLNGTITAKYTYGKPVKGDVTLTFLPLSFWGKKKNITKTFKINGSANFSFNDEEMKNVMDSSNGLSEYLDLSSPGPVEILTTVTESVTGISRNVSTNVFFKQHDYIIEFFDYTTVLKPSLNFTATVKVTRADGNQLTLEERRNNVVITVTQRNYTEYWSGSNSGNQKMEAVQKINYTVPQSGTFKIEFPILEDSSELQLKAYFLGSKSSMAVHSLFKSPSKTYIQLKTRDENIKVGSPFELVVSGNKRLKELSYMVVSRGQLVAVGKQNSTMFSLTPENSWTPKACVIVYYIEDDGEIISDVLKIPVQLVFKNKIKLYWSKVKAEPSEKVSLRISVTQPDSIVGIVAVDKSVNLMNASNDITMENVVHELELYNTGYYLGMFMNSFAVFQECGLWVLTDANLTKDYIDGVYDNAEYAERFMEENEGHIVDIHDFSLGSSPHVRKHFPETWIWLDTNMGYRIYQEFEVTVPDSITSWVATGFVISEDLGLGLTTTPVELQAFQPFFIFLNLPYSVIRGEEFALEITIFNYLKDATEVKVIIEKSDKFDILMTSNEINATGHQQTLLVPSEDGATVLFPIRPTHLGEIPITVTALSPTASDAVTQMILVKAEGIEKSYSQSILLDLTDNRLQSTLKTLSFSFPPNTVTGSERVQITAIGDVLGPSINGLASLIRMPYGCGEQNMINFAPNIYILDYLTKKKQLTDNLKEKALSFMRQGYQRELLYQREDGSFSAFGNYDPSGSTWLSAFVLRCFLEADPYIDIDQNVLHRTYTWLKGHQKSNGEFWDPGRVIHSELQGGNKSPVTLTAYIVTSLLGYRKYQPNIDVQESIHFLESEFSRGISDNYTLALITYALSSVGSPKAKEALNMLTWRAEQEGGMQFWVSSESKLSDSWQPRSLDIEVAAYALLSHFLQFQTSEGIPIMRWLSRQRNSLGGFASTQDTTVALKALSEFAALMNTERTNIQVTVTGPSSPSPVKFLIDTHNRLLLQTAELAVVQPTAVNISANGFGFAICQLNVVYNVKASGSSRRRRSIQNQEAFDLDVAVKENKDDLNHVDLNVCTSFSGPGRSGMALMEVNLLSGFMVPSEAISLSETVKKVEYDHGKLNLYLDSVNETQFCVNIPAVRNFKVSNTQDASVSIVDYYEPRRQAVRSYNSEVKLSSCDLCSDVQGCRPCEDGASGSHHHSSVIFIFCFKLLYFMELWL

Target ORF information:

RefSeq Version	XM_017010283.1
Organism	Homo sapiens(human)
Definition	Homo sapiens CD109 molecule (CD109), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010283.1

ORF Insert Sequence:

ATGAATTCTA AGCATTTAAA TGGTACCATC ACGGCAAAGT ATACATATGG GAAGCCAGTG 
AAAGGAGACG TAACGCTTAC ATTTTTACCT TTATCCTTTT GGGGAAAGAA GAAAAATATT 
ACAAAAACAT TTAAGATAAA TGGATCTGCA AACTTCTCTT TTAATGATGA AGAGATGAAA 
AATGTAATGG ATTCTTCAAA TGGACTTTCT GAATACCTGG ATCTATCTTC CCCTGGACCA 
GTAGAAATTT TAACCACAGT GACAGAATCA GTTACAGGTA TTTCAAGAAA TGTAAGCACT 
AATGTGTTCT TCAAGCAACA TGATTACATC ATTGAGTTTT TTGATTATAC TACTGTCTTG 
AAGCCATCTC TCAACTTCAC AGCCACTGTG AAGGTAACTC GTGCTGATGG CAACCAACTG 
ACTCTTGAAG AAAGAAGAAA TAATGTAGTC ATAACAGTGA CACAGAGAAA CTATACTGAG 
TACTGGAGCG GATCTAACAG TGGAAATCAG AAAATGGAAG CTGTTCAGAA AATAAATTAT 
ACTGTCCCCC AAAGTGGAAC TTTTAAGATT GAATTCCCAA TCCTGGAGGA TTCCAGTGAG 
CTACAGTTGA AGGCCTATTT CCTTGGTAGT AAAAGTAGCA TGGCAGTTCA TAGTCTGTTT 
AAGTCTCCTA GTAAGACATA CATCCAACTA AAAACAAGAG ATGAAAATAT AAAGGTGGGA 
TCGCCTTTTG AGTTGGTGGT TAGTGGCAAC AAACGATTGA AGGAGTTAAG CTATATGGTA 
GTATCCAGGG GACAGTTGGT GGCTGTAGGA AAACAAAATT CAACAATGTT CTCTTTAACA 
CCAGAAAATT CTTGGACTCC AAAAGCCTGT GTAATTGTGT ATTATATTGA AGATGATGGG 
GAAATTATAA GTGATGTTCT AAAAATTCCT GTTCAGCTTG TTTTTAAAAA TAAGATAAAG 
CTATATTGGA GTAAAGTGAA AGCTGAACCA TCTGAGAAAG TCTCTCTTAG GATCTCTGTG 
ACACAGCCTG ACTCCATAGT TGGGATTGTA GCTGTTGACA AAAGTGTGAA TCTGATGAAT 
GCCTCTAATG ATATTACAAT GGAAAATGTG GTCCATGAGT TGGAACTTTA TAACACAGGA 
TATTATTTAG GCATGTTCAT GAATTCTTTT GCAGTCTTTC AGGAATGTGG ACTCTGGGTA 
TTGACAGATG CAAACCTCAC GAAGGATTAT ATTGATGGTG TTTATGACAA TGCAGAATAT 
GCTGAGAGGT TTATGGAGGA AAATGAAGGA CATATTGTAG ATATTCATGA CTTTTCTTTG 
GGTAGCAGTC CACATGTCCG AAAGCATTTT CCAGAGACTT GGATTTGGCT AGACACCAAC 
ATGGGTTACA GGATTTACCA AGAATTTGAA GTAACTGTAC CTGATTCTAT CACTTCTTGG 
GTGGCTACTG GTTTTGTGAT CTCTGAGGAC CTGGGTCTTG GACTAACAAC TACTCCAGTG 
GAGCTCCAAG CCTTCCAACC ATTTTTCATT TTTTTGAATC TTCCCTACTC TGTTATCAGA 
GGTGAAGAAT TTGCTTTGGA AATAACTATA TTCAATTATT TGAAAGATGC CACTGAGGTT 
AAGGTAATCA TTGAGAAAAG TGACAAATTT GATATTCTAA TGACTTCAAA TGAAATAAAT 
GCCACAGGCC ACCAGCAGAC CCTTCTGGTT CCCAGTGAGG ATGGGGCAAC TGTTCTTTTT 
CCCATCAGGC CAACACATCT GGGAGAAATT CCTATCACAG TCACAGCTCT TTCACCCACT 
GCTTCTGATG CTGTCACCCA GATGATTTTA GTAAAGGCTG AAGGAATAGA AAAATCATAT 
TCACAATCCA TCTTATTAGA CTTGACTGAC AATAGGCTAC AGAGTACCCT GAAAACTTTG 
AGTTTCTCAT TTCCTCCTAA TACAGTGACT GGCAGTGAAA GAGTTCAGAT CACTGCAATT 
GGAGATGTTC TTGGTCCTTC CATCAATGGC TTAGCCTCAT TGATTCGGAT GCCTTATGGC 
TGTGGTGAAC AGAACATGAT AAATTTTGCT CCAAATATTT ACATTTTGGA TTATCTGACT 
AAAAAGAAAC AACTGACAGA TAATTTGAAA GAAAAAGCTC TTTCATTTAT GAGGCAAGGT 
TACCAGAGAG AACTTCTCTA TCAGAGGGAA GATGGCTCTT TCAGTGCTTT TGGGAATTAT 
GACCCTTCTG GGAGCACTTG GTTGTCAGCT TTTGTTTTAA GATGTTTCCT TGAAGCCGAT 
CCTTACATAG ATATTGATCA GAATGTGTTA CACAGAACAT ACACTTGGCT TAAAGGACAT 
CAGAAATCCA ACGGTGAATT TTGGGATCCA GGAAGAGTGA TTCATAGTGA GCTTCAAGGT 
GGCAATAAAA GTCCAGTAAC ACTTACAGCC TATATTGTAA CTTCTCTCCT GGGATATAGA 
AAGTATCAGC CTAACATTGA TGTGCAAGAG TCTATCCATT TTTTGGAGTC TGAATTCAGT 
AGAGGAATTT CAGACAATTA TACTCTAGCC CTTATAACTT ATGCATTGTC ATCAGTGGGG 
AGTCCTAAAG CGAAGGAAGC TTTGAATATG CTGACTTGGA GAGCAGAACA AGAAGGTGGC 
ATGCAATTCT GGGTGTCATC AGAGTCCAAA CTTTCTGACT CCTGGCAGCC ACGCTCCCTG 
GATATTGAAG TTGCAGCCTA TGCACTGCTC TCACACTTCT TACAATTTCA GACTTCTGAG 
GGAATCCCAA TTATGAGGTG GCTAAGCAGG CAAAGAAATA GCTTGGGTGG TTTTGCATCT 
ACTCAGGATA CCACTGTGGC TTTAAAGGCT CTGTCTGAAT TTGCAGCCCT AATGAATACA 
GAAAGGACAA ATATCCAAGT GACCGTGACG GGGCCTAGCT CACCAAGTCC TGTAAAGTTT 
CTGATTGACA CACACAACCG CTTACTCCTT CAGACAGCAG AGCTTGCTGT GGTACAGCCA 
ACGGCAGTTA ATATTTCCGC AAATGGTTTT GGATTTGCTA TTTGTCAGCT CAATGTTGTA 
TATAATGTGA AGGCTTCTGG GTCTTCTAGA AGACGAAGAT CTATCCAAAA TCAAGAAGCC 
TTTGATTTAG ATGTTGCTGT AAAAGAAAAT AAAGATGATC TCAATCATGT GGATTTGAAT 
GTGTGTACAA GCTTTTCGGG CCCGGGTAGG AGTGGCATGG CTCTTATGGA AGTTAACCTA 
TTAAGTGGCT TTATGGTGCC TTCAGAAGCA ATTTCTCTGA GCGAGACAGT GAAGAAAGTG 
GAATATGATC ATGGAAAACT CAACCTCTAT TTAGATTCTG TAAATGAAAC CCAGTTTTGT 
GTTAATATTC CTGCTGTGAG AAACTTTAAA GTTTCAAATA CCCAAGATGC TTCAGTGTCC 
ATAGTGGATT ACTATGAGCC AAGGAGACAG GCGGTGAGAA GTTACAACTC TGAAGTGAAG 
CTGTCCTCCT GTGACCTTTG CAGTGATGTC CAGGGCTGCC GTCCTTGTGA GGATGGAGCT 
TCAGGCTCCC ATCATCACTC TTCAGTCATT TTTATTTTCT GTTTCAAGCT TCTGTACTTT 
ATGGAACTTT GGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CD109 ( NM_133493.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_598000.2 Homo sapiens CD109 molecule (CD109), transcript variant 1, mRNA.

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CloneID	OHu17039
Clone ID Related Accession (Same CDS sequence)	XM_005248659.3 , NM_133493.4 , NM_133493.5
Accession Version	NM_133493.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-28
Organism	Homo sapiens(human)
Product	CD109 antigen isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP279681.1, AF410459.1, AK095888.1, AL834478.1, BX646244.1, BX107999.1, CB529476.1, BM722643.1, AK130104.1 and CB853216.1. This sequence is a reference standard in the RefSeqGene project. On Apr 25, 2014 this sequence version replaced NM_133493.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF410459.1, SRR1803612.249067.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGGGCC CACCGCTCCT GACCGCCGCC CACCTCCTCT GCGTGTGCAC CGCCGCGCTG 
GCCGTGGCTC CCGGGCCTCG GTTTCTGGTG ACAGCCCCAG GGATCATCAG GCCCGGAGGA 
AATGTGACTA TTGGGGTGGA GCTTCTGGAA CACTGCCCTT CACAGGTGAC TGTGAAGGCG 
GAGCTGCTCA AGACAGCATC AAACCTCACT GTCTCTGTCC TGGAAGCAGA AGGAGTCTTT 
GAAAAAGGCT CTTTTAAGAC ACTTACTCTT CCATCACTAC CTCTGAACAG TGCAGATGAG 
ATTTATGAGC TACGTGTAAC CGGACGTACC CAGGATGAGA TTTTATTCTC TAATAGTACC 
CGCTTATCAT TTGAGACCAA GAGAATATCT GTCTTCATTC AAACAGACAA GGCCTTATAC 
AAGCCAAAGC AAGAAGTGAA GTTTCGCATT GTTACACTCT TCTCAGATTT TAAGCCTTAC 
AAAACCTCTT TAAACATTCT CATTAAGGAC CCCAAATCAA ATTTGATCCA ACAGTGGTTG 
TCACAACAAA GTGATCTTGG AGTCATTTCC AAAACTTTTC AGCTATCTTC CCATCCAATA 
CTTGGTGACT GGTCTATTCA AGTTCAAGTG AATGACCAGA CATACTATCA ATCATTTCAG 
GTTTCAGAAT ATGTATTACC AAAATTTGAA GTGACTTTGC AGACACCATT ATATTGTTCT 
ATGAATTCTA AGCATTTAAA TGGTACCATC ACGGCAAAGT ATACATATGG GAAGCCAGTG 
AAAGGAGACG TAACGCTTAC ATTTTTACCT TTATCCTTTT GGGGAAAGAA GAAAAATATT 
ACAAAAACAT TTAAGATAAA TGGATCTGCA AACTTCTCTT TTAATGATGA AGAGATGAAA 
AATGTAATGG ATTCTTCAAA TGGACTTTCT GAATACCTGG ATCTATCTTC CCCTGGACCA 
GTAGAAATTT TAACCACAGT GACAGAATCA GTTACAGGTA TTTCAAGAAA TGTAAGCACT 
AATGTGTTCT TCAAGCAACA TGATTACATC ATTGAGTTTT TTGATTATAC TACTGTCTTG 
AAGCCATCTC TCAACTTCAC AGCCACTGTG AAGGTAACTC GTGCTGATGG CAACCAACTG 
ACTCTTGAAG AAAGAAGAAA TAATGTAGTC ATAACAGTGA CACAGAGAAA CTATACTGAG 
TACTGGAGCG GATCTAACAG TGGAAATCAG AAAATGGAAG CTGTTCAGAA AATAAATTAT 
ACTGTCCCCC AAAGTGGAAC TTTTAAGATT GAATTCCCAA TCCTGGAGGA TTCCAGTGAG 
CTACAGTTGA AGGCCTATTT CCTTGGTAGT AAAAGTAGCA TGGCAGTTCA TAGTCTGTTT 
AAGTCTCCTA GTAAGACATA CATCCAACTA AAAACAAGAG ATGAAAATAT AAAGGTGGGA 
TCGCCTTTTG AGTTGGTGGT TAGTGGCAAC AAACGATTGA AGGAGTTAAG CTATATGGTA 
GTATCCAGGG GACAGTTGGT GGCTGTAGGA AAACAAAATT CAACAATGTT CTCTTTAACA 
CCAGAAAATT CTTGGACTCC AAAAGCCTGT GTAATTGTGT ATTATATTGA AGATGATGGG 
GAAATTATAA GTGATGTTCT AAAAATTCCT GTTCAGCTTG TTTTTAAAAA TAAGATAAAG 
CTATATTGGA GTAAAGTGAA AGCTGAACCA TCTGAGAAAG TCTCTCTTAG GATCTCTGTG 
ACACAGCCTG ACTCCATAGT TGGGATTGTA GCTGTTGACA AAAGTGTGAA TCTGATGAAT 
GCCTCTAATG ATATTACAAT GGAAAATGTG GTCCATGAGT TGGAACTTTA TAACACAGGA 
TATTATTTAG GCATGTTCAT GAATTCTTTT GCAGTCTTTC AGGAATGTGG ACTCTGGGTA 
TTGACAGATG CAAACCTCAC GAAGGATTAT ATTGATGGTG TTTATGACAA TGCAGAATAT 
GCTGAGAGGT TTATGGAGGA AAATGAAGGA CATATTGTAG ATATTCATGA CTTTTCTTTG 
GGTAGCAGTC CACATGTCCG AAAGCATTTT CCAGAGACTT GGATTTGGCT AGACACCAAC 
ATGGGTTACA GGATTTACCA AGAATTTGAA GTAACTGTAC CTGATTCTAT CACTTCTTGG 
GTGGCTACTG GTTTTGTGAT CTCTGAGGAC CTGGGTCTTG GACTAACAAC TACTCCAGTG 
GAGCTCCAAG CCTTCCAACC ATTTTTCATT TTTTTGAATC TTCCCTACTC TGTTATCAGA 
GGTGAAGAAT TTGCTTTGGA AATAACTATA TTCAATTATT TGAAAGATGC CACTGAGGTT 
AAGGTAATCA TTGAGAAAAG TGACAAATTT GATATTCTAA TGACTTCAAA TGAAATAAAT 
GCCACAGGCC ACCAGCAGAC CCTTCTGGTT CCCAGTGAGG ATGGGGCAAC TGTTCTTTTT 
CCCATCAGGC CAACACATCT GGGAGAAATT CCTATCACAG TCACAGCTCT TTCACCCACT 
GCTTCTGATG CTGTCACCCA GATGATTTTA GTAAAGGCTG AAGGAATAGA AAAATCATAT 
TCACAATCCA TCTTATTAGA CTTGACTGAC AATAGGCTAC AGAGTACCCT GAAAACTTTG 
AGTTTCTCAT TTCCTCCTAA TACAGTGACT GGCAGTGAAA GAGTTCAGAT CACTGCAATT 
GGAGATGTTC TTGGTCCTTC CATCAATGGC TTAGCCTCAT TGATTCGGAT GCCTTATGGC 
TGTGGTGAAC AGAACATGAT AAATTTTGCT CCAAATATTT ACATTTTGGA TTATCTGACT 
AAAAAGAAAC AACTGACAGA TAATTTGAAA GAAAAAGCTC TTTCATTTAT GAGGCAAGGT 
TACCAGAGAG AACTTCTCTA TCAGAGGGAA GATGGCTCTT TCAGTGCTTT TGGGAATTAT 
GACCCTTCTG GGAGCACTTG GTTGTCAGCT TTTGTTTTAA GATGTTTCCT TGAAGCCGAT 
CCTTACATAG ATATTGATCA GAATGTGTTA CACAGAACAT ACACTTGGCT TAAAGGACAT 
CAGAAATCCA ACGGTGAATT TTGGGATCCA GGAAGAGTGA TTCATAGTGA GCTTCAAGGT 
GGCAATAAAA GTCCAGTAAC ACTTACAGCC TATATTGTAA CTTCTCTCCT GGGATATAGA 
AAGTATCAGC CTAACATTGA TGTGCAAGAG TCTATCCATT TTTTGGAGTC TGAATTCAGT 
AGAGGAATTT CAGACAATTA TACTCTAGCC CTTATAACTT ATGCATTGTC ATCAGTGGGG 
AGTCCTAAAG CGAAGGAAGC TTTGAATATG CTGACTTGGA GAGCAGAACA AGAAGGTGGC 
ATGCAATTCT GGGTGTCATC AGAGTCCAAA CTTTCTGACT CCTGGCAGCC ACGCTCCCTG 
GATATTGAAG TTGCAGCCTA TGCACTGCTC TCACACTTCT TACAATTTCA GACTTCTGAG 
GGAATCCCAA TTATGAGGTG GCTAAGCAGG CAAAGAAATA GCTTGGGTGG TTTTGCATCT 
ACTCAGGATA CCACTGTGGC TTTAAAGGCT CTGTCTGAAT TTGCAGCCCT AATGAATACA 
GAAAGGACAA ATATCCAAGT GACCGTGACG GGGCCTAGCT CACCAAGTCC TGTAAAGTTT 
CTGATTGACA CACACAACCG CTTACTCCTT CAGACAGCAG AGCTTGCTGT GGTACAGCCA 
ACGGCAGTTA ATATTTCCGC AAATGGTTTT GGATTTGCTA TTTGTCAGCT CAATGTTGTA 
TATAATGTGA AGGCTTCTGG GTCTTCTAGA AGACGAAGAT CTATCCAAAA TCAAGAAGCC 
TTTGATTTAG ATGTTGCTGT AAAAGAAAAT AAAGATGATC TCAATCATGT GGATTTGAAT 
GTGTGTACAA GCTTTTCGGG CCCGGGTAGG AGTGGCATGG CTCTTATGGA AGTTAACCTA 
TTAAGTGGCT TTATGGTGCC TTCAGAAGCA ATTTCTCTGA GCGAGACAGT GAAGAAAGTG 
GAATATGATC ATGGAAAACT CAACCTCTAT TTAGATTCTG TAAATGAAAC CCAGTTTTGT 
GTTAATATTC CTGCTGTGAG AAACTTTAAA GTTTCAAATA CCCAAGATGC TTCAGTGTCC 
ATAGTGGATT ACTATGAGCC AAGGAGACAG GCGGTGAGAA GTTACAACTC TGAAGTGAAG 
CTGTCCTCCT GTGACCTTTG CAGTGATGTC CAGGGCTGCC GTCCTTGTGA GGATGGAGCT 
TCAGGCTCCC ATCATCACTC TTCAGTCATT TTTATTTTCT GTTTCAAGCT TCTGTACTTT 
ATGGAACTTT GGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598000.2
CDS	132..4469
Translation	MQGPPLLTAAHLLCVCTAALAVAPGPRFLVTAPGIIRPGGNVTIGVELLEHCPSQVTVKAELLKTASNLTVSVLEAEGVFEKGSFKTLTLPSLPLNSADEIYELRVTGRTQDEILFSNSTRLSFETKRISVFIQTDKALYKPKQEVKFRIVTLFSDFKPYKTSLNILIKDPKSNLIQQWLSQQSDLGVISKTFQLSSHPILGDWSIQVQVNDQTYYQSFQVSEYVLPKFEVTLQTPLYCSMNSKHLNGTITAKYTYGKPVKGDVTLTFLPLSFWGKKKNITKTFKINGSANFSFNDEEMKNVMDSSNGLSEYLDLSSPGPVEILTTVTESVTGISRNVSTNVFFKQHDYIIEFFDYTTVLKPSLNFTATVKVTRADGNQLTLEERRNNVVITVTQRNYTEYWSGSNSGNQKMEAVQKINYTVPQSGTFKIEFPILEDSSELQLKAYFLGSKSSMAVHSLFKSPSKTYIQLKTRDENIKVGSPFELVVSGNKRLKELSYMVVSRGQLVAVGKQNSTMFSLTPENSWTPKACVIVYYIEDDGEIISDVLKIPVQLVFKNKIKLYWSKVKAEPSEKVSLRISVTQPDSIVGIVAVDKSVNLMNASNDITMENVVHELELYNTGYYLGMFMNSFAVFQECGLWVLTDANLTKDYIDGVYDNAEYAERFMEENEGHIVDIHDFSLGSSPHVRKHFPETWIWLDTNMGYRIYQEFEVTVPDSITSWVATGFVISEDLGLGLTTTPVELQAFQPFFIFLNLPYSVIRGEEFALEITIFNYLKDATEVKVIIEKSDKFDILMTSNEINATGHQQTLLVPSEDGATVLFPIRPTHLGEIPITVTALSPTASDAVTQMILVKAEGIEKSYSQSILLDLTDNRLQSTLKTLSFSFPPNTVTGSERVQITAIGDVLGPSINGLASLIRMPYGCGEQNMINFAPNIYILDYLTKKKQLTDNLKEKALSFMRQGYQRELLYQREDGSFSAFGNYDPSGSTWLSAFVLRCFLEADPYIDIDQNVLHRTYTWLKGHQKSNGEFWDPGRVIHSELQGGNKSPVTLTAYIVTSLLGYRKYQPNIDVQESIHFLESEFSRGISDNYTLALITYALSSVGSPKAKEALNMLTWRAEQEGGMQFWVSSESKLSDSWQPRSLDIEVAAYALLSHFLQFQTSEGIPIMRWLSRQRNSLGGFASTQDTTVALKALSEFAALMNTERTNIQVTVTGPSSPSPVKFLIDTHNRLLLQTAELAVVQPTAVNISANGFGFAICQLNVVYNVKASGSSRRRRSIQNQEAFDLDVAVKENKDDLNHVDLNVCTSFSGPGRSGMALMEVNLLSGFMVPSEAISLSETVKKVEYDHGKLNLYLDSVNETQFCVNIPAVRNFKVSNTQDASVSIVDYYEPRRQAVRSYNSEVKLSSCDLCSDVQGCRPCEDGASGSHHHSSVIFIFCFKLLYFMELWL

Target ORF information:

RefSeq Version	NM_133493.4
Organism	Homo sapiens(human)
Definition	Homo sapiens CD109 molecule (CD109), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133493.4

ORF Insert Sequence:

ATGCAGGGCC CACCGCTCCT GACCGCCGCC CACCTCCTCT GCGTGTGCAC CGCCGCGCTG 
GCCGTGGCTC CCGGGCCTCG GTTTCTGGTG ACAGCCCCAG GGATCATCAG GCCCGGAGGA 
AATGTGACTA TTGGGGTGGA GCTTCTGGAA CACTGCCCTT CACAGGTGAC TGTGAAGGCG 
GAGCTGCTCA AGACAGCATC AAACCTCACT GTCTCTGTCC TGGAAGCAGA AGGAGTCTTT 
GAAAAAGGCT CTTTTAAGAC ACTTACTCTT CCATCACTAC CTCTGAACAG TGCAGATGAG 
ATTTATGAGC TACGTGTAAC CGGACGTACC CAGGATGAGA TTTTATTCTC TAATAGTACC 
CGCTTATCAT TTGAGACCAA GAGAATATCT GTCTTCATTC AAACAGACAA GGCCTTATAC 
AAGCCAAAGC AAGAAGTGAA GTTTCGCATT GTTACACTCT TCTCAGATTT TAAGCCTTAC 
AAAACCTCTT TAAACATTCT CATTAAGGAC CCCAAATCAA ATTTGATCCA ACAGTGGTTG 
TCACAACAAA GTGATCTTGG AGTCATTTCC AAAACTTTTC AGCTATCTTC CCATCCAATA 
CTTGGTGACT GGTCTATTCA AGTTCAAGTG AATGACCAGA CATACTATCA ATCATTTCAG 
GTTTCAGAAT ATGTATTACC AAAATTTGAA GTGACTTTGC AGACACCATT ATATTGTTCT 
ATGAATTCTA AGCATTTAAA TGGTACCATC ACGGCAAAGT ATACATATGG GAAGCCAGTG 
AAAGGAGACG TAACGCTTAC ATTTTTACCT TTATCCTTTT GGGGAAAGAA GAAAAATATT 
ACAAAAACAT TTAAGATAAA TGGATCTGCA AACTTCTCTT TTAATGATGA AGAGATGAAA 
AATGTAATGG ATTCTTCAAA TGGACTTTCT GAATACCTGG ATCTATCTTC CCCTGGACCA 
GTAGAAATTT TAACCACAGT GACAGAATCA GTTACAGGTA TTTCAAGAAA TGTAAGCACT 
AATGTGTTCT TCAAGCAACA TGATTACATC ATTGAGTTTT TTGATTATAC TACTGTCTTG 
AAGCCATCTC TCAACTTCAC AGCCACTGTG AAGGTAACTC GTGCTGATGG CAACCAACTG 
ACTCTTGAAG AAAGAAGAAA TAATGTAGTC ATAACAGTGA CACAGAGAAA CTATACTGAG 
TACTGGAGCG GATCTAACAG TGGAAATCAG AAAATGGAAG CTGTTCAGAA AATAAATTAT 
ACTGTCCCCC AAAGTGGAAC TTTTAAGATT GAATTCCCAA TCCTGGAGGA TTCCAGTGAG 
CTACAGTTGA AGGCCTATTT CCTTGGTAGT AAAAGTAGCA TGGCAGTTCA TAGTCTGTTT 
AAGTCTCCTA GTAAGACATA CATCCAACTA AAAACAAGAG ATGAAAATAT AAAGGTGGGA 
TCGCCTTTTG AGTTGGTGGT TAGTGGCAAC AAACGATTGA AGGAGTTAAG CTATATGGTA 
GTATCCAGGG GACAGTTGGT GGCTGTAGGA AAACAAAATT CAACAATGTT CTCTTTAACA 
CCAGAAAATT CTTGGACTCC AAAAGCCTGT GTAATTGTGT ATTATATTGA AGATGATGGG 
GAAATTATAA GTGATGTTCT AAAAATTCCT GTTCAGCTTG TTTTTAAAAA TAAGATAAAG 
CTATATTGGA GTAAAGTGAA AGCTGAACCA TCTGAGAAAG TCTCTCTTAG GATCTCTGTG 
ACACAGCCTG ACTCCATAGT TGGGATTGTA GCTGTTGACA AAAGTGTGAA TCTGATGAAT 
GCCTCTAATG ATATTACAAT GGAAAATGTG GTCCATGAGT TGGAACTTTA TAACACAGGA 
TATTATTTAG GCATGTTCAT GAATTCTTTT GCAGTCTTTC AGGAATGTGG ACTCTGGGTA 
TTGACAGATG CAAACCTCAC GAAGGATTAT ATTGATGGTG TTTATGACAA TGCAGAATAT 
GCTGAGAGGT TTATGGAGGA AAATGAAGGA CATATTGTAG ATATTCATGA CTTTTCTTTG 
GGTAGCAGTC CACATGTCCG AAAGCATTTT CCAGAGACTT GGATTTGGCT AGACACCAAC 
ATGGGTTACA GGATTTACCA AGAATTTGAA GTAACTGTAC CTGATTCTAT CACTTCTTGG 
GTGGCTACTG GTTTTGTGAT CTCTGAGGAC CTGGGTCTTG GACTAACAAC TACTCCAGTG 
GAGCTCCAAG CCTTCCAACC ATTTTTCATT TTTTTGAATC TTCCCTACTC TGTTATCAGA 
GGTGAAGAAT TTGCTTTGGA AATAACTATA TTCAATTATT TGAAAGATGC CACTGAGGTT 
AAGGTAATCA TTGAGAAAAG TGACAAATTT GATATTCTAA TGACTTCAAA TGAAATAAAT 
GCCACAGGCC ACCAGCAGAC CCTTCTGGTT CCCAGTGAGG ATGGGGCAAC TGTTCTTTTT 
CCCATCAGGC CAACACATCT GGGAGAAATT CCTATCACAG TCACAGCTCT TTCACCCACT 
GCTTCTGATG CTGTCACCCA GATGATTTTA GTAAAGGCTG AAGGAATAGA AAAATCATAT 
TCACAATCCA TCTTATTAGA CTTGACTGAC AATAGGCTAC AGAGTACCCT GAAAACTTTG 
AGTTTCTCAT TTCCTCCTAA TACAGTGACT GGCAGTGAAA GAGTTCAGAT CACTGCAATT 
GGAGATGTTC TTGGTCCTTC CATCAATGGC TTAGCCTCAT TGATTCGGAT GCCTTATGGC 
TGTGGTGAAC AGAACATGAT AAATTTTGCT CCAAATATTT ACATTTTGGA TTATCTGACT 
AAAAAGAAAC AACTGACAGA TAATTTGAAA GAAAAAGCTC TTTCATTTAT GAGGCAAGGT 
TACCAGAGAG AACTTCTCTA TCAGAGGGAA GATGGCTCTT TCAGTGCTTT TGGGAATTAT 
GACCCTTCTG GGAGCACTTG GTTGTCAGCT TTTGTTTTAA GATGTTTCCT TGAAGCCGAT 
CCTTACATAG ATATTGATCA GAATGTGTTA CACAGAACAT ACACTTGGCT TAAAGGACAT 
CAGAAATCCA ACGGTGAATT TTGGGATCCA GGAAGAGTGA TTCATAGTGA GCTTCAAGGT 
GGCAATAAAA GTCCAGTAAC ACTTACAGCC TATATTGTAA CTTCTCTCCT GGGATATAGA 
AAGTATCAGC CTAACATTGA TGTGCAAGAG TCTATCCATT TTTTGGAGTC TGAATTCAGT 
AGAGGAATTT CAGACAATTA TACTCTAGCC CTTATAACTT ATGCATTGTC ATCAGTGGGG 
AGTCCTAAAG CGAAGGAAGC TTTGAATATG CTGACTTGGA GAGCAGAACA AGAAGGTGGC 
ATGCAATTCT GGGTGTCATC AGAGTCCAAA CTTTCTGACT CCTGGCAGCC ACGCTCCCTG 
GATATTGAAG TTGCAGCCTA TGCACTGCTC TCACACTTCT TACAATTTCA GACTTCTGAG 
GGAATCCCAA TTATGAGGTG GCTAAGCAGG CAAAGAAATA GCTTGGGTGG TTTTGCATCT 
ACTCAGGATA CCACTGTGGC TTTAAAGGCT CTGTCTGAAT TTGCAGCCCT AATGAATACA 
GAAAGGACAA ATATCCAAGT GACCGTGACG GGGCCTAGCT CACCAAGTCC TGTAAAGTTT 
CTGATTGACA CACACAACCG CTTACTCCTT CAGACAGCAG AGCTTGCTGT GGTACAGCCA 
ACGGCAGTTA ATATTTCCGC AAATGGTTTT GGATTTGCTA TTTGTCAGCT CAATGTTGTA 
TATAATGTGA AGGCTTCTGG GTCTTCTAGA AGACGAAGAT CTATCCAAAA TCAAGAAGCC 
TTTGATTTAG ATGTTGCTGT AAAAGAAAAT AAAGATGATC TCAATCATGT GGATTTGAAT 
GTGTGTACAA GCTTTTCGGG CCCGGGTAGG AGTGGCATGG CTCTTATGGA AGTTAACCTA 
TTAAGTGGCT TTATGGTGCC TTCAGAAGCA ATTTCTCTGA GCGAGACAGT GAAGAAAGTG 
GAATATGATC ATGGAAAACT CAACCTCTAT TTAGATTCTG TAAATGAAAC CCAGTTTTGT 
GTTAATATTC CTGCTGTGAG AAACTTTAAA GTTTCAAATA CCCAAGATGC TTCAGTGTCC 
ATAGTGGATT ACTATGAGCC AAGGAGACAG GCGGTGAGAA GTTACAACTC TGAAGTGAAG 
CTGTCCTCCT GTGACCTTTG CAGTGATGTC CAGGGCTGCC GTCCTTGTGA GGATGGAGCT 
TCAGGCTCCC ATCATCACTC TTCAGTCATT TTTATTTTCT GTTTCAAGCT TCTGTACTTT 
ATGGAACTTT GGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CD109 ( NM_001159587.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001153059.1 Homo sapiens CD109 molecule (CD109), transcript variant 2, mRNA.

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CloneID	OHu17040
Clone ID Related Accession (Same CDS sequence)	NM_001159587.2
Accession Version	NM_001159587.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	CD109 antigen isoform 2 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP351448.1, AY788891.1, AK095888.1, AF410459.1, AL834478.1, BX646244.1, BX107999.1, CB529476.1, BM722643.1, AK130104.1 and CB853216.1. On Apr 25, 2014 this sequence version replaced NM_001159587.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site, compared to variant 1. The resulting isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY788891.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2146982, SAMN01820697 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGGGCC CACCGCTCCT GACCGCCGCC CACCTCCTCT GCGTGTGCAC CGCCGCGCTG 
GCCGTGGCTC CCGGGCCTCG GTTTCTGGTG ACAGCCCCAG GGATCATCAG GCCCGGAGGA 
AATGTGACTA TTGGGGTGGA GCTTCTGGAA CACTGCCCTT CACAGGTGAC TGTGAAGGCG 
GAGCTGCTCA AGACAGCATC AAACCTCACT GTCTCTGTCC TGGAAGCAGA AGGAGTCTTT 
GAAAAAGGCT CTTTTAAGAC ACTTACTCTT CCATCACTAC CTCTGAACAG TGCAGATGAG 
ATTTATGAGC TACGTGTAAC CGGACGTACC CAGGATGAGA TTTTATTCTC TAATAGTACC 
CGCTTATCAT TTGAGACCAA GAGAATATCT GTCTTCATTC AAACAGACAA GGCCTTATAC 
AAGCCAAAGC AAGAAGTGAA GTTTCGCATT GTTACACTCT TCTCAGATTT TAAGCCTTAC 
AAAACCTCTT TAAACATTCT CATTAAGGAC CCCAAATCAA ATTTGATCCA ACAGTGGTTG 
TCACAACAAA GTGATCTTGG AGTCATTTCC AAAACTTTTC AGCTATCTTC CCATCCAATA 
CTTGGTGACT GGTCTATTCA AGTTCAAGTG AATGACCAGA CATACTATCA ATCATTTCAG 
GTTTCAGAAT ATGTATTACC AAAATTTGAA GTGACTTTGC AGACACCATT ATATTGTTCT 
ATGAATTCTA AGCATTTAAA TGGTACCATC ACGGCAAAGT ATACATATGG GAAGCCAGTG 
AAAGGAGACG TAACGCTTAC ATTTTTACCT TTATCCTTTT GGGGAAAGAA GAAAAATATT 
ACAAAAACAT TTAAGATAAA TGGATCTGCA AACTTCTCTT TTAATGATGA AGAGATGAAA 
AATGTAATGG ATTCTTCAAA TGGACTTTCT GAATACCTGG ATCTATCTTC CCCTGGACCA 
GTAGAAATTT TAACCACAGT GACAGAATCA GTTACAGGTA TTTCAAGAAA TGTAAGCACT 
AATGTGTTCT TCAAGCAACA TGATTACATC ATTGAGTTTT TTGATTATAC TACTGTCTTG 
AAGCCATCTC TCAACTTCAC AGCCACTGTG AAGGTAACTC GTGCTGATGG CAACCAACTG 
ACTCTTGAAG AAAGAAGAAA TAATGTAGTC ATAACAGTGA CACAGAGAAA CTATACTGAG 
TACTGGAGCG GATCTAACAG TGGAAATCAG AAAATGGAAG CTGTTCAGAA AATAAATTAT 
ACTGTCCCCC AAAGTGGAAC TTTTAAGATT GAATTCCCAA TCCTGGAGGA TTCCAGTGAG 
CTACAGTTGA AGGCCTATTT CCTTGGTAGT AAAAGTAGCA TGGCAGTTCA TAGTCTGTTT 
AAGTCTCCTA GTAAGACATA CATCCAACTA AAAACAAGAG ATGAAAATAT AAAGGTGGGA 
TCGCCTTTTG AGTTGGTGGT TAGTGGCAAC AAACGATTGA AGGAGTTAAG CTATATGGTA 
GTATCCAGGG GACAGTTGGT GGCTGTAGGA AAACAAAATT CAACAATGTT CTCTTTAACA 
CCAGAAAATT CTTGGACTCC AAAAGCCTGT GTAATTGTGT ATTATATTGA AGATGATGGG 
GAAATTATAA GTGATGTTCT AAAAATTCCT GTTCAGCTTG TTTTTAAAAA TAAGATAAAG 
CTATATTGGA GTAAAGTGAA AGCTGAACCA TCTGAGAAAG TCTCTCTTAG GATCTCTGTG 
ACACAGCCTG ACTCCATAGT TGGGATTGTA GCTGTTGACA AAAGTGTGAA TCTGATGAAT 
GCCTCTAATG ATATTACAAT GGAAAATGTG GTCCATGAGT TGGAACTTTA TAACACAGGA 
TATTATTTAG GCATGTTCAT GAATTCTTTT GCAGTCTTTC AGGAATGTGG ACTCTGGGTA 
TTGACAGATG CAAACCTCAC GAAGGATTAT ATTGATGGTG TTTATGACAA TGCAGAATAT 
GCTGAGAGGT TTATGGAGGA AAATGAAGGA CATATTGTAG ATATTCATGA CTTTTCTTTG 
GGTAGCAGTC CACATGTCCG AAAGCATTTT CCAGAGACTT GGATTTGGCT AGACACCAAC 
ATGGGTTACA GGATTTACCA AGAATTTGAA GTAACTGTAC CTGATTCTAT CACTTCTTGG 
GTGGCTACTG GTTTTGTGAT CTCTGAGGAC CTGGGTCTTG GACTAACAAC TACTCCAGTG 
GAGCTCCAAG CCTTCCAACC ATTTTTCATT TTTTTGAATC TTCCCTACTC TGTTATCAGA 
GGTGAAGAAT TTGCTTTGGA AATAACTATA TTCAATTATT TGAAAGATGC CACTGAGGTT 
AAGGTAATCA TTGAGAAAAG TGACAAATTT GATATTCTAA TGACTTCAAA TGAAATAAAT 
GCCACAGGCC ACCAGCAGAC CCTTCTGGTT CCCAGTGAGG ATGGGGCAAC TGTTCTTTTT 
CCCATCAGGC CAACACATCT GGGAGAAATT CCTATCACAG TCACAGCTCT TTCACCCACT 
GCTTCTGATG CTGTCACCCA GATGATTTTA GTAAAGGCTG AAGGAATAGA AAAATCATAT 
TCACAATCCA TCTTATTAGA CTTGACTGAC AATAGGCTAC AGAGTACCCT GAAAACTTTG 
AGTTTCTCAT TTCCTCCTAA TACAGTGACT GGCAGTGAAA GAGTTCAGAT CACTGCAATT 
GGAGATGTTC TTGGTCCTTC CATCAATGGC TTAGCCTCAT TGATTCGGAT GCCTTATGGC 
TGTGGTGAAC AGAACATGAT AAATTTTGCT CCAAATATTT ACATTTTGGA TTATCTGACT 
AAAAAGAAAC AACTGACAGA TAATTTGAAA GAAAAAGCTC TTTCATTTAT GAGGCAAGGT 
TACCAGAGAG AACTTCTCTA TCAGAGGGAA GATGGCTCTT TCAGTGCTTT TGGGAATTAT 
GACCCTTCTG GGAGCACTTG GTTGTCAGCT TTTGTTTTAA GATGTTTCCT TGAAGCCGAT 
CCTTACATAG ATATTGATCA GAATGTGTTA CACAGAACAT ACACTTGGCT TAAAGGACAT 
CAGAAATCCA ACGGTGAATT TTGGGATCCA GGAAGAGTGA TTCATAGTGA GCTTCAAGGT 
GGCAATAAAA GTCCAGTAAC ACTTACAGCC TATATTGTAA CTTCTCTCCT GGGATATAGA 
AAGTATCAGC CTAACATTGA TGTGCAAGAG TCTATCCATT TTTTGGAGTC TGAATTCAGT 
AGAGGAATTT CAGACAATTA TACTCTAGCC CTTATAACTT ATGCATTGTC ATCAGTGGGG 
AGTCCTAAAG CGAAGGAAGC TTTGAATATG CTGACTTGGA GAGCAGAACA AGAAGGTGGC 
ATGCAATTCT GGGTGTCATC AGAGTCCAAA CTTTCTGACT CCTGGCAGCC ACGCTCCCTG 
GATATTGAAG TTGCAGCCTA TGCACTGCTC TCACACTTCT TACAATTTCA GACTTCTGAG 
GGAATCCCAA TTATGAGGTG GCTAAGCAGG CAAAGAAATA GCTTGGGTGG TTTTGCATCT 
ACTCAGGATA CCACTGTGGC TTTAAAGGCT CTGTCTGAAT TTGCAGCCCT AATGAATACA 
GAAAGGACAA ATATCCAAGT GACCGTGACG GGGCCTAGCT CACCAAGTCC TCTTGCTGTG 
GTACAGCCAA CGGCAGTTAA TATTTCCGCA AATGGTTTTG GATTTGCTAT TTGTCAGCTC 
AATGTTGTAT ATAATGTGAA GGCTTCTGGG TCTTCTAGAA GACGAAGATC TATCCAAAAT 
CAAGAAGCCT TTGATTTAGA TGTTGCTGTA AAAGAAAATA AAGATGATCT CAATCATGTG 
GATTTGAATG TGTGTACAAG CTTTTCGGGC CCGGGTAGGA GTGGCATGGC TCTTATGGAA 
GTTAACCTAT TAAGTGGCTT TATGGTGCCT TCAGAAGCAA TTTCTCTGAG CGAGACAGTG 
AAGAAAGTGG AATATGATCA TGGAAAACTC AACCTCTATT TAGATTCTGT AAATGAAACC 
CAGTTTTGTG TTAATATTCC TGCTGTGAGA AACTTTAAAG TTTCAAATAC CCAAGATGCT 
TCAGTGTCCA TAGTGGATTA CTATGAGCCA AGGAGACAGG CGGTGAGAAG TTACAACTCT 
GAAGTGAAGC TGTCCTCCTG TGACCTTTGC AGTGATGTCC AGGGCTGCCG TCCTTGTGAG 
GATGGAGCTT CAGGCTCCCA TCATCACTCT TCAGTCATTT TTATTTTCTG TTTCAAGCTT 
CTGTACTTTA TGGAACTTTG GCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153059.1
CDS	132..4418
Translation	MQGPPLLTAAHLLCVCTAALAVAPGPRFLVTAPGIIRPGGNVTIGVELLEHCPSQVTVKAELLKTASNLTVSVLEAEGVFEKGSFKTLTLPSLPLNSADEIYELRVTGRTQDEILFSNSTRLSFETKRISVFIQTDKALYKPKQEVKFRIVTLFSDFKPYKTSLNILIKDPKSNLIQQWLSQQSDLGVISKTFQLSSHPILGDWSIQVQVNDQTYYQSFQVSEYVLPKFEVTLQTPLYCSMNSKHLNGTITAKYTYGKPVKGDVTLTFLPLSFWGKKKNITKTFKINGSANFSFNDEEMKNVMDSSNGLSEYLDLSSPGPVEILTTVTESVTGISRNVSTNVFFKQHDYIIEFFDYTTVLKPSLNFTATVKVTRADGNQLTLEERRNNVVITVTQRNYTEYWSGSNSGNQKMEAVQKINYTVPQSGTFKIEFPILEDSSELQLKAYFLGSKSSMAVHSLFKSPSKTYIQLKTRDENIKVGSPFELVVSGNKRLKELSYMVVSRGQLVAVGKQNSTMFSLTPENSWTPKACVIVYYIEDDGEIISDVLKIPVQLVFKNKIKLYWSKVKAEPSEKVSLRISVTQPDSIVGIVAVDKSVNLMNASNDITMENVVHELELYNTGYYLGMFMNSFAVFQECGLWVLTDANLTKDYIDGVYDNAEYAERFMEENEGHIVDIHDFSLGSSPHVRKHFPETWIWLDTNMGYRIYQEFEVTVPDSITSWVATGFVISEDLGLGLTTTPVELQAFQPFFIFLNLPYSVIRGEEFALEITIFNYLKDATEVKVIIEKSDKFDILMTSNEINATGHQQTLLVPSEDGATVLFPIRPTHLGEIPITVTALSPTASDAVTQMILVKAEGIEKSYSQSILLDLTDNRLQSTLKTLSFSFPPNTVTGSERVQITAIGDVLGPSINGLASLIRMPYGCGEQNMINFAPNIYILDYLTKKKQLTDNLKEKALSFMRQGYQRELLYQREDGSFSAFGNYDPSGSTWLSAFVLRCFLEADPYIDIDQNVLHRTYTWLKGHQKSNGEFWDPGRVIHSELQGGNKSPVTLTAYIVTSLLGYRKYQPNIDVQESIHFLESEFSRGISDNYTLALITYALSSVGSPKAKEALNMLTWRAEQEGGMQFWVSSESKLSDSWQPRSLDIEVAAYALLSHFLQFQTSEGIPIMRWLSRQRNSLGGFASTQDTTVALKALSEFAALMNTERTNIQVTVTGPSSPSPLAVVQPTAVNISANGFGFAICQLNVVYNVKASGSSRRRRSIQNQEAFDLDVAVKENKDDLNHVDLNVCTSFSGPGRSGMALMEVNLLSGFMVPSEAISLSETVKKVEYDHGKLNLYLDSVNETQFCVNIPAVRNFKVSNTQDASVSIVDYYEPRRQAVRSYNSEVKLSSCDLCSDVQGCRPCEDGASGSHHHSSVIFIFCFKLLYFMELWL

Target ORF information:

RefSeq Version	NM_001159587.2
Organism	Homo sapiens(human)
Definition	Homo sapiens CD109 molecule (CD109), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001159587.2

ORF Insert Sequence:

ATGCAGGGCC CACCGCTCCT GACCGCCGCC CACCTCCTCT GCGTGTGCAC CGCCGCGCTG 
GCCGTGGCTC CCGGGCCTCG GTTTCTGGTG ACAGCCCCAG GGATCATCAG GCCCGGAGGA 
AATGTGACTA TTGGGGTGGA GCTTCTGGAA CACTGCCCTT CACAGGTGAC TGTGAAGGCG 
GAGCTGCTCA AGACAGCATC AAACCTCACT GTCTCTGTCC TGGAAGCAGA AGGAGTCTTT 
GAAAAAGGCT CTTTTAAGAC ACTTACTCTT CCATCACTAC CTCTGAACAG TGCAGATGAG 
ATTTATGAGC TACGTGTAAC CGGACGTACC CAGGATGAGA TTTTATTCTC TAATAGTACC 
CGCTTATCAT TTGAGACCAA GAGAATATCT GTCTTCATTC AAACAGACAA GGCCTTATAC 
AAGCCAAAGC AAGAAGTGAA GTTTCGCATT GTTACACTCT TCTCAGATTT TAAGCCTTAC 
AAAACCTCTT TAAACATTCT CATTAAGGAC CCCAAATCAA ATTTGATCCA ACAGTGGTTG 
TCACAACAAA GTGATCTTGG AGTCATTTCC AAAACTTTTC AGCTATCTTC CCATCCAATA 
CTTGGTGACT GGTCTATTCA AGTTCAAGTG AATGACCAGA CATACTATCA ATCATTTCAG 
GTTTCAGAAT ATGTATTACC AAAATTTGAA GTGACTTTGC AGACACCATT ATATTGTTCT 
ATGAATTCTA AGCATTTAAA TGGTACCATC ACGGCAAAGT ATACATATGG GAAGCCAGTG 
AAAGGAGACG TAACGCTTAC ATTTTTACCT TTATCCTTTT GGGGAAAGAA GAAAAATATT 
ACAAAAACAT TTAAGATAAA TGGATCTGCA AACTTCTCTT TTAATGATGA AGAGATGAAA 
AATGTAATGG ATTCTTCAAA TGGACTTTCT GAATACCTGG ATCTATCTTC CCCTGGACCA 
GTAGAAATTT TAACCACAGT GACAGAATCA GTTACAGGTA TTTCAAGAAA TGTAAGCACT 
AATGTGTTCT TCAAGCAACA TGATTACATC ATTGAGTTTT TTGATTATAC TACTGTCTTG 
AAGCCATCTC TCAACTTCAC AGCCACTGTG AAGGTAACTC GTGCTGATGG CAACCAACTG 
ACTCTTGAAG AAAGAAGAAA TAATGTAGTC ATAACAGTGA CACAGAGAAA CTATACTGAG 
TACTGGAGCG GATCTAACAG TGGAAATCAG AAAATGGAAG CTGTTCAGAA AATAAATTAT 
ACTGTCCCCC AAAGTGGAAC TTTTAAGATT GAATTCCCAA TCCTGGAGGA TTCCAGTGAG 
CTACAGTTGA AGGCCTATTT CCTTGGTAGT AAAAGTAGCA TGGCAGTTCA TAGTCTGTTT 
AAGTCTCCTA GTAAGACATA CATCCAACTA AAAACAAGAG ATGAAAATAT AAAGGTGGGA 
TCGCCTTTTG AGTTGGTGGT TAGTGGCAAC AAACGATTGA AGGAGTTAAG CTATATGGTA 
GTATCCAGGG GACAGTTGGT GGCTGTAGGA AAACAAAATT CAACAATGTT CTCTTTAACA 
CCAGAAAATT CTTGGACTCC AAAAGCCTGT GTAATTGTGT ATTATATTGA AGATGATGGG 
GAAATTATAA GTGATGTTCT AAAAATTCCT GTTCAGCTTG TTTTTAAAAA TAAGATAAAG 
CTATATTGGA GTAAAGTGAA AGCTGAACCA TCTGAGAAAG TCTCTCTTAG GATCTCTGTG 
ACACAGCCTG ACTCCATAGT TGGGATTGTA GCTGTTGACA AAAGTGTGAA TCTGATGAAT 
GCCTCTAATG ATATTACAAT GGAAAATGTG GTCCATGAGT TGGAACTTTA TAACACAGGA 
TATTATTTAG GCATGTTCAT GAATTCTTTT GCAGTCTTTC AGGAATGTGG ACTCTGGGTA 
TTGACAGATG CAAACCTCAC GAAGGATTAT ATTGATGGTG TTTATGACAA TGCAGAATAT 
GCTGAGAGGT TTATGGAGGA AAATGAAGGA CATATTGTAG ATATTCATGA CTTTTCTTTG 
GGTAGCAGTC CACATGTCCG AAAGCATTTT CCAGAGACTT GGATTTGGCT AGACACCAAC 
ATGGGTTACA GGATTTACCA AGAATTTGAA GTAACTGTAC CTGATTCTAT CACTTCTTGG 
GTGGCTACTG GTTTTGTGAT CTCTGAGGAC CTGGGTCTTG GACTAACAAC TACTCCAGTG 
GAGCTCCAAG CCTTCCAACC ATTTTTCATT TTTTTGAATC TTCCCTACTC TGTTATCAGA 
GGTGAAGAAT TTGCTTTGGA AATAACTATA TTCAATTATT TGAAAGATGC CACTGAGGTT 
AAGGTAATCA TTGAGAAAAG TGACAAATTT GATATTCTAA TGACTTCAAA TGAAATAAAT 
GCCACAGGCC ACCAGCAGAC CCTTCTGGTT CCCAGTGAGG ATGGGGCAAC TGTTCTTTTT 
CCCATCAGGC CAACACATCT GGGAGAAATT CCTATCACAG TCACAGCTCT TTCACCCACT 
GCTTCTGATG CTGTCACCCA GATGATTTTA GTAAAGGCTG AAGGAATAGA AAAATCATAT 
TCACAATCCA TCTTATTAGA CTTGACTGAC AATAGGCTAC AGAGTACCCT GAAAACTTTG 
AGTTTCTCAT TTCCTCCTAA TACAGTGACT GGCAGTGAAA GAGTTCAGAT CACTGCAATT 
GGAGATGTTC TTGGTCCTTC CATCAATGGC TTAGCCTCAT TGATTCGGAT GCCTTATGGC 
TGTGGTGAAC AGAACATGAT AAATTTTGCT CCAAATATTT ACATTTTGGA TTATCTGACT 
AAAAAGAAAC AACTGACAGA TAATTTGAAA GAAAAAGCTC TTTCATTTAT GAGGCAAGGT 
TACCAGAGAG AACTTCTCTA TCAGAGGGAA GATGGCTCTT TCAGTGCTTT TGGGAATTAT 
GACCCTTCTG GGAGCACTTG GTTGTCAGCT TTTGTTTTAA GATGTTTCCT TGAAGCCGAT 
CCTTACATAG ATATTGATCA GAATGTGTTA CACAGAACAT ACACTTGGCT TAAAGGACAT 
CAGAAATCCA ACGGTGAATT TTGGGATCCA GGAAGAGTGA TTCATAGTGA GCTTCAAGGT 
GGCAATAAAA GTCCAGTAAC ACTTACAGCC TATATTGTAA CTTCTCTCCT GGGATATAGA 
AAGTATCAGC CTAACATTGA TGTGCAAGAG TCTATCCATT TTTTGGAGTC TGAATTCAGT 
AGAGGAATTT CAGACAATTA TACTCTAGCC CTTATAACTT ATGCATTGTC ATCAGTGGGG 
AGTCCTAAAG CGAAGGAAGC TTTGAATATG CTGACTTGGA GAGCAGAACA AGAAGGTGGC 
ATGCAATTCT GGGTGTCATC AGAGTCCAAA CTTTCTGACT CCTGGCAGCC ACGCTCCCTG 
GATATTGAAG TTGCAGCCTA TGCACTGCTC TCACACTTCT TACAATTTCA GACTTCTGAG 
GGAATCCCAA TTATGAGGTG GCTAAGCAGG CAAAGAAATA GCTTGGGTGG TTTTGCATCT 
ACTCAGGATA CCACTGTGGC TTTAAAGGCT CTGTCTGAAT TTGCAGCCCT AATGAATACA 
GAAAGGACAA ATATCCAAGT GACCGTGACG GGGCCTAGCT CACCAAGTCC TCTTGCTGTG 
GTACAGCCAA CGGCAGTTAA TATTTCCGCA AATGGTTTTG GATTTGCTAT TTGTCAGCTC 
AATGTTGTAT ATAATGTGAA GGCTTCTGGG TCTTCTAGAA GACGAAGATC TATCCAAAAT 
CAAGAAGCCT TTGATTTAGA TGTTGCTGTA AAAGAAAATA AAGATGATCT CAATCATGTG 
GATTTGAATG TGTGTACAAG CTTTTCGGGC CCGGGTAGGA GTGGCATGGC TCTTATGGAA 
GTTAACCTAT TAAGTGGCTT TATGGTGCCT TCAGAAGCAA TTTCTCTGAG CGAGACAGTG 
AAGAAAGTGG AATATGATCA TGGAAAACTC AACCTCTATT TAGATTCTGT AAATGAAACC 
CAGTTTTGTG TTAATATTCC TGCTGTGAGA AACTTTAAAG TTTCAAATAC CCAAGATGCT 
TCAGTGTCCA TAGTGGATTA CTATGAGCCA AGGAGACAGG CGGTGAGAAG TTACAACTCT 
GAAGTGAAGC TGTCCTCCTG TGACCTTTGC AGTGATGTCC AGGGCTGCCG TCCTTGTGAG 
GATGGAGCTT CAGGCTCCCA TCATCACTCT TCAGTCATTT TTATTTTCTG TTTCAAGCTT 
CTGTACTTTA TGGAACTTTG GCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CD109 ( NM_001159588.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001153060.1 Homo sapiens CD109 molecule (CD109), transcript variant 3, mRNA.

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CloneID	OHu17041
Clone ID Related Accession (Same CDS sequence)	NM_001159588.2
Accession Version	NM_001159588.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	CD109 antigen isoform 3 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP351448.1, BX641095.1, AL834478.1, BX646244.1, BX107999.1, CB529476.1, BM722643.1, AK130104.1 and CB853216.1. On Apr 25, 2014 this sequence version replaced NM_001159588.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. The resulting isoform (3) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX641095.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154125, SAMEA2467144 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGGGCC CACCGCTCCT GACCGCCGCC CACCTCCTCT GCGTGTGCAC CGCCGCGCTG 
GCCGTGGCTC CCGGGCCTCG GTTTCTGGTG ACAGCCCCAG GGATCATCAG GCCCGGAGGA 
AATGTGACTA TTGGGGTGGA GCTTCTGGAA CACTGCCCTT CACAGGTGAC TGTGAAGGCG 
GAGCTGCTCA AGACAGCATC AAACCTCACT GTCTCTGTCC TGGAAGCAGA AGGAGTCTTT 
GAAAAAGGCT CTTTTAAGAC ACTTACTCTT CCATCAGACC CCAAATCAAA TTTGATCCAA 
CAGTGGTTGT CACAACAAAG TGATCTTGGA GTCATTTCCA AAACTTTTCA GCTATCTTCC 
CATCCAATAC TTGGTGACTG GTCTATTCAA GTTCAAGTGA ATGACCAGAC ATACTATCAA 
TCATTTCAGG TTTCAGAATA TGTATTACCA AAATTTGAAG TGACTTTGCA GACACCATTA 
TATTGTTCTA TGAATTCTAA GCATTTAAAT GGTACCATCA CGGCAAAGTA TACATATGGG 
AAGCCAGTGA AAGGAGACGT AACGCTTACA TTTTTACCTT TATCCTTTTG GGGAAAGAAG 
AAAAATATTA CAAAAACATT TAAGATAAAT GGATCTGCAA ACTTCTCTTT TAATGATGAA 
GAGATGAAAA ATGTAATGGA TTCTTCAAAT GGACTTTCTG AATACCTGGA TCTATCTTCC 
CCTGGACCAG TAGAAATTTT AACCACAGTG ACAGAATCAG TTACAGGTAT TTCAAGAAAT 
GTAAGCACTA ATGTGTTCTT CAAGCAACAT GATTACATCA TTGAGTTTTT TGATTATACT 
ACTGTCTTGA AGCCATCTCT CAACTTCACA GCCACTGTGA AGGTAACTCG TGCTGATGGC 
AACCAACTGA CTCTTGAAGA AAGAAGAAAT AATGTAGTCA TAACAGTGAC ACAGAGAAAC 
TATACTGAGT ACTGGAGCGG ATCTAACAGT GGAAATCAGA AAATGGAAGC TGTTCAGAAA 
ATAAATTATA CTGTCCCCCA AAGTGGAACT TTTAAGATTG AATTCCCAAT CCTGGAGGAT 
TCCAGTGAGC TACAGTTGAA GGCCTATTTC CTTGGTAGTA AAAGTAGCAT GGCAGTTCAT 
AGTCTGTTTA AGTCTCCTAG TAAGACATAC ATCCAACTAA AAACAAGAGA TGAAAATATA 
AAGGTGGGAT CGCCTTTTGA GTTGGTGGTT AGTGGCAACA AACGATTGAA GGAGTTAAGC 
TATATGGTAG TATCCAGGGG ACAGTTGGTG GCTGTAGGAA AACAAAATTC AACAATGTTC 
TCTTTAACAC CAGAAAATTC TTGGACTCCA AAAGCCTGTG TAATTGTGTA TTATATTGAA 
GATGATGGGG AAATTATAAG TGATGTTCTA AAAATTCCTG TTCAGCTTGT TTTTAAAAAT 
AAGATAAAGC TATATTGGAG TAAAGTGAAA GCTGAACCAT CTGAGAAAGT CTCTCTTAGG 
ATCTCTGTGA CACAGCCTGA CTCCATAGTT GGGATTGTAG CTGTTGACAA AAGTGTGAAT 
CTGATGAATG CCTCTAATGA TATTACAATG GAAAATGTGG TCCATGAGTT GGAACTTTAT 
AACACAGGAT ATTATTTAGG CATGTTCATG AATTCTTTTG CAGTCTTTCA GGAATGTGGA 
CTCTGGGTAT TGACAGATGC AAACCTCACG AAGGATTATA TTGATGGTGT TTATGACAAT 
GCAGAATATG CTGAGAGGTT TATGGAGGAA AATGAAGGAC ATATTGTAGA TATTCATGAC 
TTTTCTTTGG GTAGCAGTCC ACATGTCCGA AAGCATTTTC CAGAGACTTG GATTTGGCTA 
GACACCAACA TGGGTTACAG GATTTACCAA GAATTTGAAG TAACTGTACC TGATTCTATC 
ACTTCTTGGG TGGCTACTGG TTTTGTGATC TCTGAGGACC TGGGTCTTGG ACTAACAACT 
ACTCCAGTGG AGCTCCAAGC CTTCCAACCA TTTTTCATTT TTTTGAATCT TCCCTACTCT 
GTTATCAGAG GTGAAGAATT TGCTTTGGAA ATAACTATAT TCAATTATTT GAAAGATGCC 
ACTGAGGTTA AGGTAATCAT TGAGAAAAGT GACAAATTTG ATATTCTAAT GACTTCAAAT 
GAAATAAATG CCACAGGCCA CCAGCAGACC CTTCTGGTTC CCAGTGAGGA TGGGGCAACT 
GTTCTTTTTC CCATCAGGCC AACACATCTG GGAGAAATTC CTATCACAGT CACAGCTCTT 
TCACCCACTG CTTCTGATGC TGTCACCCAG ATGATTTTAG TAAAGGCTGA AGGAATAGAA 
AAATCATATT CACAATCCAT CTTATTAGAC TTGACTGACA ATAGGCTACA GAGTACCCTG 
AAAACTTTGA GTTTCTCATT TCCTCCTAAT ACAGTGACTG GCAGTGAAAG AGTTCAGATC 
ACTGCAATTG GAGATGTTCT TGGTCCTTCC ATCAATGGCT TAGCCTCATT GATTCGGATG 
CCTTATGGCT GTGGTGAACA GAACATGATA AATTTTGCTC CAAATATTTA CATTTTGGAT 
TATCTGACTA AAAAGAAACA ACTGACAGAT AATTTGAAAG AAAAAGCTCT TTCATTTATG 
AGGCAAGGTT ACCAGAGAGA ACTTCTCTAT CAGAGGGAAG ATGGCTCTTT CAGTGCTTTT 
GGGAATTATG ACCCTTCTGG GAGCACTTGG TTGTCAGCTT TTGTTTTAAG ATGTTTCCTT 
GAAGCCGATC CTTACATAGA TATTGATCAG AATGTGTTAC ACAGAACATA CACTTGGCTT 
AAAGGACATC AGAAATCCAA CGGTGAATTT TGGGATCCAG GAAGAGTGAT TCATAGTGAG 
CTTCAAGGTG GCAATAAAAG TCCAGTAACA CTTACAGCCT ATATTGTAAC TTCTCTCCTG 
GGATATAGAA AGTATCAGCC TAACATTGAT GTGCAAGAGT CTATCCATTT TTTGGAGTCT 
GAATTCAGTA GAGGAATTTC AGACAATTAT ACTCTAGCCC TTATAACTTA TGCATTGTCA 
TCAGTGGGGA GTCCTAAAGC GAAGGAAGCT TTGAATATGC TGACTTGGAG AGCAGAACAA 
GAAGGTGGCA TGCAATTCTG GGTGTCATCA GAGTCCAAAC TTTCTGACTC CTGGCAGCCA 
CGCTCCCTGG ATATTGAAGT TGCAGCCTAT GCACTGCTCT CACACTTCTT ACAATTTCAG 
ACTTCTGAGG GAATCCCAAT TATGAGGTGG CTAAGCAGGC AAAGAAATAG CTTGGGTGGT 
TTTGCATCTA CTCAGGATAC CACTGTGGCT TTAAAGGCTC TGTCTGAATT TGCAGCCCTA 
ATGAATACAG AAAGGACAAA TATCCAAGTG ACCGTGACGG GGCCTAGCTC ACCAAGTCCT 
GTAAAGTTTC TGATTGACAC ACACAACCGC TTACTCCTTC AGACAGCAGA GCTTGCTGTG 
GTACAGCCAA CGGCAGTTAA TATTTCCGCA AATGGTTTTG GATTTGCTAT TTGTCAGCTC 
AATGTTGTAT ATAATGTGAA GGCTTCTGGG TCTTCTAGAA GACGAAGATC TATCCAAAAT 
CAAGAAGCCT TTGATTTAGA TGTTGCTGTA AAAGAAAATA AAGATGATCT CAATCATGTG 
GATTTGAATG TGTGTACAAG CTTTTCGGGC CCGGGTAGGA GTGGCATGGC TCTTATGGAA 
GTTAACCTAT TAAGTGGCTT TATGGTGCCT TCAGAAGCAA TTTCTCTGAG CGAGACAGTG 
AAGAAAGTGG AATATGATCA TGGAAAACTC AACCTCTATT TAGATTCTGT AAATGAAACC 
CAGTTTTGTG TTAATATTCC TGCTGTGAGA AACTTTAAAG TTTCAAATAC CCAAGATGCT 
TCAGTGTCCA TAGTGGATTA CTATGAGCCA AGGAGACAGG CGGTGAGAAG TTACAACTCT 
GAAGTGAAGC TGTCCTCCTG TGACCTTTGC AGTGATGTCC AGGGCTGCCG TCCTTGTGAG 
GATGGAGCTT CAGGCTCCCA TCATCACTCT TCAGTCATTT TTATTTTCTG TTTCAAGCTT 
CTGTACTTTA TGGAACTTTG GCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153060.1
CDS	132..4238
Translation	MQGPPLLTAAHLLCVCTAALAVAPGPRFLVTAPGIIRPGGNVTIGVELLEHCPSQVTVKAELLKTASNLTVSVLEAEGVFEKGSFKTLTLPSDPKSNLIQQWLSQQSDLGVISKTFQLSSHPILGDWSIQVQVNDQTYYQSFQVSEYVLPKFEVTLQTPLYCSMNSKHLNGTITAKYTYGKPVKGDVTLTFLPLSFWGKKKNITKTFKINGSANFSFNDEEMKNVMDSSNGLSEYLDLSSPGPVEILTTVTESVTGISRNVSTNVFFKQHDYIIEFFDYTTVLKPSLNFTATVKVTRADGNQLTLEERRNNVVITVTQRNYTEYWSGSNSGNQKMEAVQKINYTVPQSGTFKIEFPILEDSSELQLKAYFLGSKSSMAVHSLFKSPSKTYIQLKTRDENIKVGSPFELVVSGNKRLKELSYMVVSRGQLVAVGKQNSTMFSLTPENSWTPKACVIVYYIEDDGEIISDVLKIPVQLVFKNKIKLYWSKVKAEPSEKVSLRISVTQPDSIVGIVAVDKSVNLMNASNDITMENVVHELELYNTGYYLGMFMNSFAVFQECGLWVLTDANLTKDYIDGVYDNAEYAERFMEENEGHIVDIHDFSLGSSPHVRKHFPETWIWLDTNMGYRIYQEFEVTVPDSITSWVATGFVISEDLGLGLTTTPVELQAFQPFFIFLNLPYSVIRGEEFALEITIFNYLKDATEVKVIIEKSDKFDILMTSNEINATGHQQTLLVPSEDGATVLFPIRPTHLGEIPITVTALSPTASDAVTQMILVKAEGIEKSYSQSILLDLTDNRLQSTLKTLSFSFPPNTVTGSERVQITAIGDVLGPSINGLASLIRMPYGCGEQNMINFAPNIYILDYLTKKKQLTDNLKEKALSFMRQGYQRELLYQREDGSFSAFGNYDPSGSTWLSAFVLRCFLEADPYIDIDQNVLHRTYTWLKGHQKSNGEFWDPGRVIHSELQGGNKSPVTLTAYIVTSLLGYRKYQPNIDVQESIHFLESEFSRGISDNYTLALITYALSSVGSPKAKEALNMLTWRAEQEGGMQFWVSSESKLSDSWQPRSLDIEVAAYALLSHFLQFQTSEGIPIMRWLSRQRNSLGGFASTQDTTVALKALSEFAALMNTERTNIQVTVTGPSSPSPVKFLIDTHNRLLLQTAELAVVQPTAVNISANGFGFAICQLNVVYNVKASGSSRRRRSIQNQEAFDLDVAVKENKDDLNHVDLNVCTSFSGPGRSGMALMEVNLLSGFMVPSEAISLSETVKKVEYDHGKLNLYLDSVNETQFCVNIPAVRNFKVSNTQDASVSIVDYYEPRRQAVRSYNSEVKLSSCDLCSDVQGCRPCEDGASGSHHHSSVIFIFCFKLLYFMELWL

Target ORF information:

RefSeq Version	NM_001159588.2
Organism	Homo sapiens(human)
Definition	Homo sapiens CD109 molecule (CD109), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001159588.2

ORF Insert Sequence:

ATGCAGGGCC CACCGCTCCT GACCGCCGCC CACCTCCTCT GCGTGTGCAC CGCCGCGCTG 
GCCGTGGCTC CCGGGCCTCG GTTTCTGGTG ACAGCCCCAG GGATCATCAG GCCCGGAGGA 
AATGTGACTA TTGGGGTGGA GCTTCTGGAA CACTGCCCTT CACAGGTGAC TGTGAAGGCG 
GAGCTGCTCA AGACAGCATC AAACCTCACT GTCTCTGTCC TGGAAGCAGA AGGAGTCTTT 
GAAAAAGGCT CTTTTAAGAC ACTTACTCTT CCATCAGACC CCAAATCAAA TTTGATCCAA 
CAGTGGTTGT CACAACAAAG TGATCTTGGA GTCATTTCCA AAACTTTTCA GCTATCTTCC 
CATCCAATAC TTGGTGACTG GTCTATTCAA GTTCAAGTGA ATGACCAGAC ATACTATCAA 
TCATTTCAGG TTTCAGAATA TGTATTACCA AAATTTGAAG TGACTTTGCA GACACCATTA 
TATTGTTCTA TGAATTCTAA GCATTTAAAT GGTACCATCA CGGCAAAGTA TACATATGGG 
AAGCCAGTGA AAGGAGACGT AACGCTTACA TTTTTACCTT TATCCTTTTG GGGAAAGAAG 
AAAAATATTA CAAAAACATT TAAGATAAAT GGATCTGCAA ACTTCTCTTT TAATGATGAA 
GAGATGAAAA ATGTAATGGA TTCTTCAAAT GGACTTTCTG AATACCTGGA TCTATCTTCC 
CCTGGACCAG TAGAAATTTT AACCACAGTG ACAGAATCAG TTACAGGTAT TTCAAGAAAT 
GTAAGCACTA ATGTGTTCTT CAAGCAACAT GATTACATCA TTGAGTTTTT TGATTATACT 
ACTGTCTTGA AGCCATCTCT CAACTTCACA GCCACTGTGA AGGTAACTCG TGCTGATGGC 
AACCAACTGA CTCTTGAAGA AAGAAGAAAT AATGTAGTCA TAACAGTGAC ACAGAGAAAC 
TATACTGAGT ACTGGAGCGG ATCTAACAGT GGAAATCAGA AAATGGAAGC TGTTCAGAAA 
ATAAATTATA CTGTCCCCCA AAGTGGAACT TTTAAGATTG AATTCCCAAT CCTGGAGGAT 
TCCAGTGAGC TACAGTTGAA GGCCTATTTC CTTGGTAGTA AAAGTAGCAT GGCAGTTCAT 
AGTCTGTTTA AGTCTCCTAG TAAGACATAC ATCCAACTAA AAACAAGAGA TGAAAATATA 
AAGGTGGGAT CGCCTTTTGA GTTGGTGGTT AGTGGCAACA AACGATTGAA GGAGTTAAGC 
TATATGGTAG TATCCAGGGG ACAGTTGGTG GCTGTAGGAA AACAAAATTC AACAATGTTC 
TCTTTAACAC CAGAAAATTC TTGGACTCCA AAAGCCTGTG TAATTGTGTA TTATATTGAA 
GATGATGGGG AAATTATAAG TGATGTTCTA AAAATTCCTG TTCAGCTTGT TTTTAAAAAT 
AAGATAAAGC TATATTGGAG TAAAGTGAAA GCTGAACCAT CTGAGAAAGT CTCTCTTAGG 
ATCTCTGTGA CACAGCCTGA CTCCATAGTT GGGATTGTAG CTGTTGACAA AAGTGTGAAT 
CTGATGAATG CCTCTAATGA TATTACAATG GAAAATGTGG TCCATGAGTT GGAACTTTAT 
AACACAGGAT ATTATTTAGG CATGTTCATG AATTCTTTTG CAGTCTTTCA GGAATGTGGA 
CTCTGGGTAT TGACAGATGC AAACCTCACG AAGGATTATA TTGATGGTGT TTATGACAAT 
GCAGAATATG CTGAGAGGTT TATGGAGGAA AATGAAGGAC ATATTGTAGA TATTCATGAC 
TTTTCTTTGG GTAGCAGTCC ACATGTCCGA AAGCATTTTC CAGAGACTTG GATTTGGCTA 
GACACCAACA TGGGTTACAG GATTTACCAA GAATTTGAAG TAACTGTACC TGATTCTATC 
ACTTCTTGGG TGGCTACTGG TTTTGTGATC TCTGAGGACC TGGGTCTTGG ACTAACAACT 
ACTCCAGTGG AGCTCCAAGC CTTCCAACCA TTTTTCATTT TTTTGAATCT TCCCTACTCT 
GTTATCAGAG GTGAAGAATT TGCTTTGGAA ATAACTATAT TCAATTATTT GAAAGATGCC 
ACTGAGGTTA AGGTAATCAT TGAGAAAAGT GACAAATTTG ATATTCTAAT GACTTCAAAT 
GAAATAAATG CCACAGGCCA CCAGCAGACC CTTCTGGTTC CCAGTGAGGA TGGGGCAACT 
GTTCTTTTTC CCATCAGGCC AACACATCTG GGAGAAATTC CTATCACAGT CACAGCTCTT 
TCACCCACTG CTTCTGATGC TGTCACCCAG ATGATTTTAG TAAAGGCTGA AGGAATAGAA 
AAATCATATT CACAATCCAT CTTATTAGAC TTGACTGACA ATAGGCTACA GAGTACCCTG 
AAAACTTTGA GTTTCTCATT TCCTCCTAAT ACAGTGACTG GCAGTGAAAG AGTTCAGATC 
ACTGCAATTG GAGATGTTCT TGGTCCTTCC ATCAATGGCT TAGCCTCATT GATTCGGATG 
CCTTATGGCT GTGGTGAACA GAACATGATA AATTTTGCTC CAAATATTTA CATTTTGGAT 
TATCTGACTA AAAAGAAACA ACTGACAGAT AATTTGAAAG AAAAAGCTCT TTCATTTATG 
AGGCAAGGTT ACCAGAGAGA ACTTCTCTAT CAGAGGGAAG ATGGCTCTTT CAGTGCTTTT 
GGGAATTATG ACCCTTCTGG GAGCACTTGG TTGTCAGCTT TTGTTTTAAG ATGTTTCCTT 
GAAGCCGATC CTTACATAGA TATTGATCAG AATGTGTTAC ACAGAACATA CACTTGGCTT 
AAAGGACATC AGAAATCCAA CGGTGAATTT TGGGATCCAG GAAGAGTGAT TCATAGTGAG 
CTTCAAGGTG GCAATAAAAG TCCAGTAACA CTTACAGCCT ATATTGTAAC TTCTCTCCTG 
GGATATAGAA AGTATCAGCC TAACATTGAT GTGCAAGAGT CTATCCATTT TTTGGAGTCT 
GAATTCAGTA GAGGAATTTC AGACAATTAT ACTCTAGCCC TTATAACTTA TGCATTGTCA 
TCAGTGGGGA GTCCTAAAGC GAAGGAAGCT TTGAATATGC TGACTTGGAG AGCAGAACAA 
GAAGGTGGCA TGCAATTCTG GGTGTCATCA GAGTCCAAAC TTTCTGACTC CTGGCAGCCA 
CGCTCCCTGG ATATTGAAGT TGCAGCCTAT GCACTGCTCT CACACTTCTT ACAATTTCAG 
ACTTCTGAGG GAATCCCAAT TATGAGGTGG CTAAGCAGGC AAAGAAATAG CTTGGGTGGT 
TTTGCATCTA CTCAGGATAC CACTGTGGCT TTAAAGGCTC TGTCTGAATT TGCAGCCCTA 
ATGAATACAG AAAGGACAAA TATCCAAGTG ACCGTGACGG GGCCTAGCTC ACCAAGTCCT 
GTAAAGTTTC TGATTGACAC ACACAACCGC TTACTCCTTC AGACAGCAGA GCTTGCTGTG 
GTACAGCCAA CGGCAGTTAA TATTTCCGCA AATGGTTTTG GATTTGCTAT TTGTCAGCTC 
AATGTTGTAT ATAATGTGAA GGCTTCTGGG TCTTCTAGAA GACGAAGATC TATCCAAAAT 
CAAGAAGCCT TTGATTTAGA TGTTGCTGTA AAAGAAAATA AAGATGATCT CAATCATGTG 
GATTTGAATG TGTGTACAAG CTTTTCGGGC CCGGGTAGGA GTGGCATGGC TCTTATGGAA 
GTTAACCTAT TAAGTGGCTT TATGGTGCCT TCAGAAGCAA TTTCTCTGAG CGAGACAGTG 
AAGAAAGTGG AATATGATCA TGGAAAACTC AACCTCTATT TAGATTCTGT AAATGAAACC 
CAGTTTTGTG TTAATATTCC TGCTGTGAGA AACTTTAAAG TTTCAAATAC CCAAGATGCT 
TCAGTGTCCA TAGTGGATTA CTATGAGCCA AGGAGACAGG CGGTGAGAAG TTACAACTCT 
GAAGTGAAGC TGTCCTCCTG TGACCTTTGC AGTGATGTCC AGGGCTGCCG TCCTTGTGAG 
GATGGAGCTT CAGGCTCCCA TCATCACTCT TCAGTCATTT TTATTTTCTG TTTCAAGCTT 
CTGTACTTTA TGGAACTTTG GCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CD109 ( XM_005248659.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005248716.1 Homo sapiens CD109 molecule (CD109), transcript variant X1, mRNA.

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CloneID	OHu17039
Clone ID Related Accession (Same CDS sequence)	XM_005248659.3 , NM_133493.4 , NM_133493.5
Accession Version	XM_005248659.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	CD109 antigen isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005248659.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGGCC CACCGCTCCT GACCGCCGCC CACCTCCTCT GCGTGTGCAC CGCCGCGCTG 
GCCGTGGCTC CCGGGCCTCG GTTTCTGGTG ACAGCCCCAG GGATCATCAG GCCCGGAGGA 
AATGTGACTA TTGGGGTGGA GCTTCTGGAA CACTGCCCTT CACAGGTGAC TGTGAAGGCG 
GAGCTGCTCA AGACAGCATC AAACCTCACT GTCTCTGTCC TGGAAGCAGA AGGAGTCTTT 
GAAAAAGGCT CTTTTAAGAC ACTTACTCTT CCATCACTAC CTCTGAACAG TGCAGATGAG 
ATTTATGAGC TACGTGTAAC CGGACGTACC CAGGATGAGA TTTTATTCTC TAATAGTACC 
CGCTTATCAT TTGAGACCAA GAGAATATCT GTCTTCATTC AAACAGACAA GGCCTTATAC 
AAGCCAAAGC AAGAAGTGAA GTTTCGCATT GTTACACTCT TCTCAGATTT TAAGCCTTAC 
AAAACCTCTT TAAACATTCT CATTAAGGAC CCCAAATCAA ATTTGATCCA ACAGTGGTTG 
TCACAACAAA GTGATCTTGG AGTCATTTCC AAAACTTTTC AGCTATCTTC CCATCCAATA 
CTTGGTGACT GGTCTATTCA AGTTCAAGTG AATGACCAGA CATACTATCA ATCATTTCAG 
GTTTCAGAAT ATGTATTACC AAAATTTGAA GTGACTTTGC AGACACCATT ATATTGTTCT 
ATGAATTCTA AGCATTTAAA TGGTACCATC ACGGCAAAGT ATACATATGG GAAGCCAGTG 
AAAGGAGACG TAACGCTTAC ATTTTTACCT TTATCCTTTT GGGGAAAGAA GAAAAATATT 
ACAAAAACAT TTAAGATAAA TGGATCTGCA AACTTCTCTT TTAATGATGA AGAGATGAAA 
AATGTAATGG ATTCTTCAAA TGGACTTTCT GAATACCTGG ATCTATCTTC CCCTGGACCA 
GTAGAAATTT TAACCACAGT GACAGAATCA GTTACAGGTA TTTCAAGAAA TGTAAGCACT 
AATGTGTTCT TCAAGCAACA TGATTACATC ATTGAGTTTT TTGATTATAC TACTGTCTTG 
AAGCCATCTC TCAACTTCAC AGCCACTGTG AAGGTAACTC GTGCTGATGG CAACCAACTG 
ACTCTTGAAG AAAGAAGAAA TAATGTAGTC ATAACAGTGA CACAGAGAAA CTATACTGAG 
TACTGGAGCG GATCTAACAG TGGAAATCAG AAAATGGAAG CTGTTCAGAA AATAAATTAT 
ACTGTCCCCC AAAGTGGAAC TTTTAAGATT GAATTCCCAA TCCTGGAGGA TTCCAGTGAG 
CTACAGTTGA AGGCCTATTT CCTTGGTAGT AAAAGTAGCA TGGCAGTTCA TAGTCTGTTT 
AAGTCTCCTA GTAAGACATA CATCCAACTA AAAACAAGAG ATGAAAATAT AAAGGTGGGA 
TCGCCTTTTG AGTTGGTGGT TAGTGGCAAC AAACGATTGA AGGAGTTAAG CTATATGGTA 
GTATCCAGGG GACAGTTGGT GGCTGTAGGA AAACAAAATT CAACAATGTT CTCTTTAACA 
CCAGAAAATT CTTGGACTCC AAAAGCCTGT GTAATTGTGT ATTATATTGA AGATGATGGG 
GAAATTATAA GTGATGTTCT AAAAATTCCT GTTCAGCTTG TTTTTAAAAA TAAGATAAAG 
CTATATTGGA GTAAAGTGAA AGCTGAACCA TCTGAGAAAG TCTCTCTTAG GATCTCTGTG 
ACACAGCCTG ACTCCATAGT TGGGATTGTA GCTGTTGACA AAAGTGTGAA TCTGATGAAT 
GCCTCTAATG ATATTACAAT GGAAAATGTG GTCCATGAGT TGGAACTTTA TAACACAGGA 
TATTATTTAG GCATGTTCAT GAATTCTTTT GCAGTCTTTC AGGAATGTGG ACTCTGGGTA 
TTGACAGATG CAAACCTCAC GAAGGATTAT ATTGATGGTG TTTATGACAA TGCAGAATAT 
GCTGAGAGGT TTATGGAGGA AAATGAAGGA CATATTGTAG ATATTCATGA CTTTTCTTTG 
GGTAGCAGTC CACATGTCCG AAAGCATTTT CCAGAGACTT GGATTTGGCT AGACACCAAC 
ATGGGTTACA GGATTTACCA AGAATTTGAA GTAACTGTAC CTGATTCTAT CACTTCTTGG 
GTGGCTACTG GTTTTGTGAT CTCTGAGGAC CTGGGTCTTG GACTAACAAC TACTCCAGTG 
GAGCTCCAAG CCTTCCAACC ATTTTTCATT TTTTTGAATC TTCCCTACTC TGTTATCAGA 
GGTGAAGAAT TTGCTTTGGA AATAACTATA TTCAATTATT TGAAAGATGC CACTGAGGTT 
AAGGTAATCA TTGAGAAAAG TGACAAATTT GATATTCTAA TGACTTCAAA TGAAATAAAT 
GCCACAGGCC ACCAGCAGAC CCTTCTGGTT CCCAGTGAGG ATGGGGCAAC TGTTCTTTTT 
CCCATCAGGC CAACACATCT GGGAGAAATT CCTATCACAG TCACAGCTCT TTCACCCACT 
GCTTCTGATG CTGTCACCCA GATGATTTTA GTAAAGGCTG AAGGAATAGA AAAATCATAT 
TCACAATCCA TCTTATTAGA CTTGACTGAC AATAGGCTAC AGAGTACCCT GAAAACTTTG 
AGTTTCTCAT TTCCTCCTAA TACAGTGACT GGCAGTGAAA GAGTTCAGAT CACTGCAATT 
GGAGATGTTC TTGGTCCTTC CATCAATGGC TTAGCCTCAT TGATTCGGAT GCCTTATGGC 
TGTGGTGAAC AGAACATGAT AAATTTTGCT CCAAATATTT ACATTTTGGA TTATCTGACT 
AAAAAGAAAC AACTGACAGA TAATTTGAAA GAAAAAGCTC TTTCATTTAT GAGGCAAGGT 
TACCAGAGAG AACTTCTCTA TCAGAGGGAA GATGGCTCTT TCAGTGCTTT TGGGAATTAT 
GACCCTTCTG GGAGCACTTG GTTGTCAGCT TTTGTTTTAA GATGTTTCCT TGAAGCCGAT 
CCTTACATAG ATATTGATCA GAATGTGTTA CACAGAACAT ACACTTGGCT TAAAGGACAT 
CAGAAATCCA ACGGTGAATT TTGGGATCCA GGAAGAGTGA TTCATAGTGA GCTTCAAGGT 
GGCAATAAAA GTCCAGTAAC ACTTACAGCC TATATTGTAA CTTCTCTCCT GGGATATAGA 
AAGTATCAGC CTAACATTGA TGTGCAAGAG TCTATCCATT TTTTGGAGTC TGAATTCAGT 
AGAGGAATTT CAGACAATTA TACTCTAGCC CTTATAACTT ATGCATTGTC ATCAGTGGGG 
AGTCCTAAAG CGAAGGAAGC TTTGAATATG CTGACTTGGA GAGCAGAACA AGAAGGTGGC 
ATGCAATTCT GGGTGTCATC AGAGTCCAAA CTTTCTGACT CCTGGCAGCC ACGCTCCCTG 
GATATTGAAG TTGCAGCCTA TGCACTGCTC TCACACTTCT TACAATTTCA GACTTCTGAG 
GGAATCCCAA TTATGAGGTG GCTAAGCAGG CAAAGAAATA GCTTGGGTGG TTTTGCATCT 
ACTCAGGATA CCACTGTGGC TTTAAAGGCT CTGTCTGAAT TTGCAGCCCT AATGAATACA 
GAAAGGACAA ATATCCAAGT GACCGTGACG GGGCCTAGCT CACCAAGTCC TGTAAAGTTT 
CTGATTGACA CACACAACCG CTTACTCCTT CAGACAGCAG AGCTTGCTGT GGTACAGCCA 
ACGGCAGTTA ATATTTCCGC AAATGGTTTT GGATTTGCTA TTTGTCAGCT CAATGTTGTA 
TATAATGTGA AGGCTTCTGG GTCTTCTAGA AGACGAAGAT CTATCCAAAA TCAAGAAGCC 
TTTGATTTAG ATGTTGCTGT AAAAGAAAAT AAAGATGATC TCAATCATGT GGATTTGAAT 
GTGTGTACAA GCTTTTCGGG CCCGGGTAGG AGTGGCATGG CTCTTATGGA AGTTAACCTA 
TTAAGTGGCT TTATGGTGCC TTCAGAAGCA ATTTCTCTGA GCGAGACAGT GAAGAAAGTG 
GAATATGATC ATGGAAAACT CAACCTCTAT TTAGATTCTG TAAATGAAAC CCAGTTTTGT 
GTTAATATTC CTGCTGTGAG AAACTTTAAA GTTTCAAATA CCCAAGATGC TTCAGTGTCC 
ATAGTGGATT ACTATGAGCC AAGGAGACAG GCGGTGAGAA GTTACAACTC TGAAGTGAAG 
CTGTCCTCCT GTGACCTTTG CAGTGATGTC CAGGGCTGCC GTCCTTGTGA GGATGGAGCT 
TCAGGCTCCC ATCATCACTC TTCAGTCATT TTTATTTTCT GTTTCAAGCT TCTGTACTTT 
ATGGAACTTT GGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005248716.1
CDS	279..4616
Translation	MQGPPLLTAAHLLCVCTAALAVAPGPRFLVTAPGIIRPGGNVTIGVELLEHCPSQVTVKAELLKTASNLTVSVLEAEGVFEKGSFKTLTLPSLPLNSADEIYELRVTGRTQDEILFSNSTRLSFETKRISVFIQTDKALYKPKQEVKFRIVTLFSDFKPYKTSLNILIKDPKSNLIQQWLSQQSDLGVISKTFQLSSHPILGDWSIQVQVNDQTYYQSFQVSEYVLPKFEVTLQTPLYCSMNSKHLNGTITAKYTYGKPVKGDVTLTFLPLSFWGKKKNITKTFKINGSANFSFNDEEMKNVMDSSNGLSEYLDLSSPGPVEILTTVTESVTGISRNVSTNVFFKQHDYIIEFFDYTTVLKPSLNFTATVKVTRADGNQLTLEERRNNVVITVTQRNYTEYWSGSNSGNQKMEAVQKINYTVPQSGTFKIEFPILEDSSELQLKAYFLGSKSSMAVHSLFKSPSKTYIQLKTRDENIKVGSPFELVVSGNKRLKELSYMVVSRGQLVAVGKQNSTMFSLTPENSWTPKACVIVYYIEDDGEIISDVLKIPVQLVFKNKIKLYWSKVKAEPSEKVSLRISVTQPDSIVGIVAVDKSVNLMNASNDITMENVVHELELYNTGYYLGMFMNSFAVFQECGLWVLTDANLTKDYIDGVYDNAEYAERFMEENEGHIVDIHDFSLGSSPHVRKHFPETWIWLDTNMGYRIYQEFEVTVPDSITSWVATGFVISEDLGLGLTTTPVELQAFQPFFIFLNLPYSVIRGEEFALEITIFNYLKDATEVKVIIEKSDKFDILMTSNEINATGHQQTLLVPSEDGATVLFPIRPTHLGEIPITVTALSPTASDAVTQMILVKAEGIEKSYSQSILLDLTDNRLQSTLKTLSFSFPPNTVTGSERVQITAIGDVLGPSINGLASLIRMPYGCGEQNMINFAPNIYILDYLTKKKQLTDNLKEKALSFMRQGYQRELLYQREDGSFSAFGNYDPSGSTWLSAFVLRCFLEADPYIDIDQNVLHRTYTWLKGHQKSNGEFWDPGRVIHSELQGGNKSPVTLTAYIVTSLLGYRKYQPNIDVQESIHFLESEFSRGISDNYTLALITYALSSVGSPKAKEALNMLTWRAEQEGGMQFWVSSESKLSDSWQPRSLDIEVAAYALLSHFLQFQTSEGIPIMRWLSRQRNSLGGFASTQDTTVALKALSEFAALMNTERTNIQVTVTGPSSPSPVKFLIDTHNRLLLQTAELAVVQPTAVNISANGFGFAICQLNVVYNVKASGSSRRRRSIQNQEAFDLDVAVKENKDDLNHVDLNVCTSFSGPGRSGMALMEVNLLSGFMVPSEAISLSETVKKVEYDHGKLNLYLDSVNETQFCVNIPAVRNFKVSNTQDASVSIVDYYEPRRQAVRSYNSEVKLSSCDLCSDVQGCRPCEDGASGSHHHSSVIFIFCFKLLYFMELWL

Target ORF information:

RefSeq Version	XM_005248659.3
Organism	Homo sapiens(human)
Definition	Homo sapiens CD109 molecule (CD109), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005248659.3

ORF Insert Sequence:

ATGCAGGGCC CACCGCTCCT GACCGCCGCC CACCTCCTCT GCGTGTGCAC CGCCGCGCTG 
GCCGTGGCTC CCGGGCCTCG GTTTCTGGTG ACAGCCCCAG GGATCATCAG GCCCGGAGGA 
AATGTGACTA TTGGGGTGGA GCTTCTGGAA CACTGCCCTT CACAGGTGAC TGTGAAGGCG 
GAGCTGCTCA AGACAGCATC AAACCTCACT GTCTCTGTCC TGGAAGCAGA AGGAGTCTTT 
GAAAAAGGCT CTTTTAAGAC ACTTACTCTT CCATCACTAC CTCTGAACAG TGCAGATGAG 
ATTTATGAGC TACGTGTAAC CGGACGTACC CAGGATGAGA TTTTATTCTC TAATAGTACC 
CGCTTATCAT TTGAGACCAA GAGAATATCT GTCTTCATTC AAACAGACAA GGCCTTATAC 
AAGCCAAAGC AAGAAGTGAA GTTTCGCATT GTTACACTCT TCTCAGATTT TAAGCCTTAC 
AAAACCTCTT TAAACATTCT CATTAAGGAC CCCAAATCAA ATTTGATCCA ACAGTGGTTG 
TCACAACAAA GTGATCTTGG AGTCATTTCC AAAACTTTTC AGCTATCTTC CCATCCAATA 
CTTGGTGACT GGTCTATTCA AGTTCAAGTG AATGACCAGA CATACTATCA ATCATTTCAG 
GTTTCAGAAT ATGTATTACC AAAATTTGAA GTGACTTTGC AGACACCATT ATATTGTTCT 
ATGAATTCTA AGCATTTAAA TGGTACCATC ACGGCAAAGT ATACATATGG GAAGCCAGTG 
AAAGGAGACG TAACGCTTAC ATTTTTACCT TTATCCTTTT GGGGAAAGAA GAAAAATATT 
ACAAAAACAT TTAAGATAAA TGGATCTGCA AACTTCTCTT TTAATGATGA AGAGATGAAA 
AATGTAATGG ATTCTTCAAA TGGACTTTCT GAATACCTGG ATCTATCTTC CCCTGGACCA 
GTAGAAATTT TAACCACAGT GACAGAATCA GTTACAGGTA TTTCAAGAAA TGTAAGCACT 
AATGTGTTCT TCAAGCAACA TGATTACATC ATTGAGTTTT TTGATTATAC TACTGTCTTG 
AAGCCATCTC TCAACTTCAC AGCCACTGTG AAGGTAACTC GTGCTGATGG CAACCAACTG 
ACTCTTGAAG AAAGAAGAAA TAATGTAGTC ATAACAGTGA CACAGAGAAA CTATACTGAG 
TACTGGAGCG GATCTAACAG TGGAAATCAG AAAATGGAAG CTGTTCAGAA AATAAATTAT 
ACTGTCCCCC AAAGTGGAAC TTTTAAGATT GAATTCCCAA TCCTGGAGGA TTCCAGTGAG 
CTACAGTTGA AGGCCTATTT CCTTGGTAGT AAAAGTAGCA TGGCAGTTCA TAGTCTGTTT 
AAGTCTCCTA GTAAGACATA CATCCAACTA AAAACAAGAG ATGAAAATAT AAAGGTGGGA 
TCGCCTTTTG AGTTGGTGGT TAGTGGCAAC AAACGATTGA AGGAGTTAAG CTATATGGTA 
GTATCCAGGG GACAGTTGGT GGCTGTAGGA AAACAAAATT CAACAATGTT CTCTTTAACA 
CCAGAAAATT CTTGGACTCC AAAAGCCTGT GTAATTGTGT ATTATATTGA AGATGATGGG 
GAAATTATAA GTGATGTTCT AAAAATTCCT GTTCAGCTTG TTTTTAAAAA TAAGATAAAG 
CTATATTGGA GTAAAGTGAA AGCTGAACCA TCTGAGAAAG TCTCTCTTAG GATCTCTGTG 
ACACAGCCTG ACTCCATAGT TGGGATTGTA GCTGTTGACA AAAGTGTGAA TCTGATGAAT 
GCCTCTAATG ATATTACAAT GGAAAATGTG GTCCATGAGT TGGAACTTTA TAACACAGGA 
TATTATTTAG GCATGTTCAT GAATTCTTTT GCAGTCTTTC AGGAATGTGG ACTCTGGGTA 
TTGACAGATG CAAACCTCAC GAAGGATTAT ATTGATGGTG TTTATGACAA TGCAGAATAT 
GCTGAGAGGT TTATGGAGGA AAATGAAGGA CATATTGTAG ATATTCATGA CTTTTCTTTG 
GGTAGCAGTC CACATGTCCG AAAGCATTTT CCAGAGACTT GGATTTGGCT AGACACCAAC 
ATGGGTTACA GGATTTACCA AGAATTTGAA GTAACTGTAC CTGATTCTAT CACTTCTTGG 
GTGGCTACTG GTTTTGTGAT CTCTGAGGAC CTGGGTCTTG GACTAACAAC TACTCCAGTG 
GAGCTCCAAG CCTTCCAACC ATTTTTCATT TTTTTGAATC TTCCCTACTC TGTTATCAGA 
GGTGAAGAAT TTGCTTTGGA AATAACTATA TTCAATTATT TGAAAGATGC CACTGAGGTT 
AAGGTAATCA TTGAGAAAAG TGACAAATTT GATATTCTAA TGACTTCAAA TGAAATAAAT 
GCCACAGGCC ACCAGCAGAC CCTTCTGGTT CCCAGTGAGG ATGGGGCAAC TGTTCTTTTT 
CCCATCAGGC CAACACATCT GGGAGAAATT CCTATCACAG TCACAGCTCT TTCACCCACT 
GCTTCTGATG CTGTCACCCA GATGATTTTA GTAAAGGCTG AAGGAATAGA AAAATCATAT 
TCACAATCCA TCTTATTAGA CTTGACTGAC AATAGGCTAC AGAGTACCCT GAAAACTTTG 
AGTTTCTCAT TTCCTCCTAA TACAGTGACT GGCAGTGAAA GAGTTCAGAT CACTGCAATT 
GGAGATGTTC TTGGTCCTTC CATCAATGGC TTAGCCTCAT TGATTCGGAT GCCTTATGGC 
TGTGGTGAAC AGAACATGAT AAATTTTGCT CCAAATATTT ACATTTTGGA TTATCTGACT 
AAAAAGAAAC AACTGACAGA TAATTTGAAA GAAAAAGCTC TTTCATTTAT GAGGCAAGGT 
TACCAGAGAG AACTTCTCTA TCAGAGGGAA GATGGCTCTT TCAGTGCTTT TGGGAATTAT 
GACCCTTCTG GGAGCACTTG GTTGTCAGCT TTTGTTTTAA GATGTTTCCT TGAAGCCGAT 
CCTTACATAG ATATTGATCA GAATGTGTTA CACAGAACAT ACACTTGGCT TAAAGGACAT 
CAGAAATCCA ACGGTGAATT TTGGGATCCA GGAAGAGTGA TTCATAGTGA GCTTCAAGGT 
GGCAATAAAA GTCCAGTAAC ACTTACAGCC TATATTGTAA CTTCTCTCCT GGGATATAGA 
AAGTATCAGC CTAACATTGA TGTGCAAGAG TCTATCCATT TTTTGGAGTC TGAATTCAGT 
AGAGGAATTT CAGACAATTA TACTCTAGCC CTTATAACTT ATGCATTGTC ATCAGTGGGG 
AGTCCTAAAG CGAAGGAAGC TTTGAATATG CTGACTTGGA GAGCAGAACA AGAAGGTGGC 
ATGCAATTCT GGGTGTCATC AGAGTCCAAA CTTTCTGACT CCTGGCAGCC ACGCTCCCTG 
GATATTGAAG TTGCAGCCTA TGCACTGCTC TCACACTTCT TACAATTTCA GACTTCTGAG 
GGAATCCCAA TTATGAGGTG GCTAAGCAGG CAAAGAAATA GCTTGGGTGG TTTTGCATCT 
ACTCAGGATA CCACTGTGGC TTTAAAGGCT CTGTCTGAAT TTGCAGCCCT AATGAATACA 
GAAAGGACAA ATATCCAAGT GACCGTGACG GGGCCTAGCT CACCAAGTCC TGTAAAGTTT 
CTGATTGACA CACACAACCG CTTACTCCTT CAGACAGCAG AGCTTGCTGT GGTACAGCCA 
ACGGCAGTTA ATATTTCCGC AAATGGTTTT GGATTTGCTA TTTGTCAGCT CAATGTTGTA 
TATAATGTGA AGGCTTCTGG GTCTTCTAGA AGACGAAGAT CTATCCAAAA TCAAGAAGCC 
TTTGATTTAG ATGTTGCTGT AAAAGAAAAT AAAGATGATC TCAATCATGT GGATTTGAAT 
GTGTGTACAA GCTTTTCGGG CCCGGGTAGG AGTGGCATGG CTCTTATGGA AGTTAACCTA 
TTAAGTGGCT TTATGGTGCC TTCAGAAGCA ATTTCTCTGA GCGAGACAGT GAAGAAAGTG 
GAATATGATC ATGGAAAACT CAACCTCTAT TTAGATTCTG TAAATGAAAC CCAGTTTTGT 
GTTAATATTC CTGCTGTGAG AAACTTTAAA GTTTCAAATA CCCAAGATGC TTCAGTGTCC 
ATAGTGGATT ACTATGAGCC AAGGAGACAG GCGGTGAGAA GTTACAACTC TGAAGTGAAG 
CTGTCCTCCT GTGACCTTTG CAGTGATGTC CAGGGCTGCC GTCCTTGTGA GGATGGAGCT 
TCAGGCTCCC ATCATCACTC TTCAGTCATT TTTATTTTCT GTTTCAAGCT TCTGTACTTT 
ATGGAACTTT GGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CD109 ( XM_011535473.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011533775.1 Homo sapiens CD109 molecule (CD109), transcript variant X2, mRNA.

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CloneID	OHu75772
Clone ID Related Accession (Same CDS sequence)	XM_011535473.1
Accession Version	XM_011535473.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	CD109 antigen isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCAAG TGTGTGTGAA CAACGCAGGC TCAGGGCCTC GGTTTCTGGT GACAGCCCCA 
GGGATCATCA GGCCCGGAGG AAATGTGACT ATTGGGGTGG AGCTTCTGGA ACACTGCCCT 
TCACAGGTGA CTGTGAAGGC GGAGCTGCTC AAGACAGCAT CAAACCTCAC TGTCTCTGTC 
CTGGAAGCAG AAGGAGTCTT TGAAAAAGGC TCTTTTAAGA CACTTACTCT TCCATCACTA 
CCTCTGAACA GTGCAGATGA GATTTATGAG CTACGTGTAA CCGGACGTAC CCAGGATGAG 
ATTTTATTCT CTAATAGTAC CCGCTTATCA TTTGAGACCA AGAGAATATC TGTCTTCATT 
CAAACAGACA AGGCCTTATA CAAGCCAAAG CAAGAAGTGA AGTTTCGCAT TGTTACACTC 
TTCTCAGATT TTAAGCCTTA CAAAACCTCT TTAAACATTC TCATTAAGGA CCCCAAATCA 
AATTTGATCC AACAGTGGTT GTCACAACAA AGTGATCTTG GAGTCATTTC CAAAACTTTT 
CAGCTATCTT CCCATCCAAT ACTTGGTGAC TGGTCTATTC AAGTTCAAGT GAATGACCAG 
ACATACTATC AATCATTTCA GGTTTCAGAA TATGTATTAC CAAAATTTGA AGTGACTTTG 
CAGACACCAT TATATTGTTC TATGAATTCT AAGCATTTAA ATGGTACCAT CACGGCAAAG 
TATACATATG GGAAGCCAGT GAAAGGAGAC GTAACGCTTA CATTTTTACC TTTATCCTTT 
TGGGGAAAGA AGAAAAATAT TACAAAAACA TTTAAGATAA ATGGATCTGC AAACTTCTCT 
TTTAATGATG AAGAGATGAA AAATGTAATG GATTCTTCAA ATGGACTTTC TGAATACCTG 
GATCTATCTT CCCCTGGACC AGTAGAAATT TTAACCACAG TGACAGAATC AGTTACAGGT 
ATTTCAAGAA ATGTAAGCAC TAATGTGTTC TTCAAGCAAC ATGATTACAT CATTGAGTTT 
TTTGATTATA CTACTGTCTT GAAGCCATCT CTCAACTTCA CAGCCACTGT GAAGGTAACT 
CGTGCTGATG GCAACCAACT GACTCTTGAA GAAAGAAGAA ATAATGTAGT CATAACAGTG 
ACACAGAGAA ACTATACTGA GTACTGGAGC GGATCTAACA GTGGAAATCA GAAAATGGAA 
GCTGTTCAGA AAATAAATTA TACTGTCCCC CAAAGTGGAA CTTTTAAGAT TGAATTCCCA 
ATCCTGGAGG ATTCCAGTGA GCTACAGTTG AAGGCCTATT TCCTTGGTAG TAAAAGTAGC 
ATGGCAGTTC ATAGTCTGTT TAAGTCTCCT AGTAAGACAT ACATCCAACT AAAAACAAGA 
GATGAAAATA TAAAGGTGGG ATCGCCTTTT GAGTTGGTGG TTAGTGGCAA CAAACGATTG 
AAGGAGTTAA GCTATATGGT AGTATCCAGG GGACAGTTGG TGGCTGTAGG AAAACAAAAT 
TCAACAATGT TCTCTTTAAC ACCAGAAAAT TCTTGGACTC CAAAAGCCTG TGTAATTGTG 
TATTATATTG AAGATGATGG GGAAATTATA AGTGATGTTC TAAAAATTCC TGTTCAGCTT 
GTTTTTAAAA ATAAGATAAA GCTATATTGG AGTAAAGTGA AAGCTGAACC ATCTGAGAAA 
GTCTCTCTTA GGATCTCTGT GACACAGCCT GACTCCATAG TTGGGATTGT AGCTGTTGAC 
AAAAGTGTGA ATCTGATGAA TGCCTCTAAT GATATTACAA TGGAAAATGT GGTCCATGAG 
TTGGAACTTT ATAACACAGG ATATTATTTA GGCATGTTCA TGAATTCTTT TGCAGTCTTT 
CAGGAATGTG GACTCTGGGT ATTGACAGAT GCAAACCTCA CGAAGGATTA TATTGATGGT 
GTTTATGACA ATGCAGAATA TGCTGAGAGG TTTATGGAGG AAAATGAAGG ACATATTGTA 
GATATTCATG ACTTTTCTTT GGGTAGCAGT CCACATGTCC GAAAGCATTT TCCAGAGACT 
TGGATTTGGC TAGACACCAA CATGGGTTAC AGGATTTACC AAGAATTTGA AGTAACTGTA 
CCTGATTCTA TCACTTCTTG GGTGGCTACT GGTTTTGTGA TCTCTGAGGA CCTGGGTCTT 
GGACTAACAA CTACTCCAGT GGAGCTCCAA GCCTTCCAAC CATTTTTCAT TTTTTTGAAT 
CTTCCCTACT CTGTTATCAG AGGTGAAGAA TTTGCTTTGG AAATAACTAT ATTCAATTAT 
TTGAAAGATG CCACTGAGGT TAAGGTAATC ATTGAGAAAA GTGACAAATT TGATATTCTA 
ATGACTTCAA ATGAAATAAA TGCCACAGGC CACCAGCAGA CCCTTCTGGT TCCCAGTGAG 
GATGGGGCAA CTGTTCTTTT TCCCATCAGG CCAACACATC TGGGAGAAAT TCCTATCACA 
GTCACAGCTC TTTCACCCAC TGCTTCTGAT GCTGTCACCC AGATGATTTT AGTAAAGGCT 
GAAGGAATAG AAAAATCATA TTCACAATCC ATCTTATTAG ACTTGACTGA CAATAGGCTA 
CAGAGTACCC TGAAAACTTT GAGTTTCTCA TTTCCTCCTA ATACAGTGAC TGGCAGTGAA 
AGAGTTCAGA TCACTGCAAT TGGAGATGTT CTTGGTCCTT CCATCAATGG CTTAGCCTCA 
TTGATTCGGA TGCCTTATGG CTGTGGTGAA CAGAACATGA TAAATTTTGC TCCAAATATT 
TACATTTTGG ATTATCTGAC TAAAAAGAAA CAACTGACAG ATAATTTGAA AGAAAAAGCT 
CTTTCATTTA TGAGGCAAGG TTACCAGAGA GAACTTCTCT ATCAGAGGGA AGATGGCTCT 
TTCAGTGCTT TTGGGAATTA TGACCCTTCT GGGAGCACTT GGTTGTCAGC TTTTGTTTTA 
AGATGTTTCC TTGAAGCCGA TCCTTACATA GATATTGATC AGAATGTGTT ACACAGAACA 
TACACTTGGC TTAAAGGACA TCAGAAATCC AACGGTGAAT TTTGGGATCC AGGAAGAGTG 
ATTCATAGTG AGCTTCAAGG TGGCAATAAA AGTCCAGTAA CACTTACAGC CTATATTGTA 
ACTTCTCTCC TGGGATATAG AAAGTATCAG CCTAACATTG ATGTGCAAGA GTCTATCCAT 
TTTTTGGAGT CTGAATTCAG TAGAGGAATT TCAGACAATT ATACTCTAGC CCTTATAACT 
TATGCATTGT CATCAGTGGG GAGTCCTAAA GCGAAGGAAG CTTTGAATAT GCTGACTTGG 
AGAGCAGAAC AAGAAGGTGG CATGCAATTC TGGGTGTCAT CAGAGTCCAA ACTTTCTGAC 
TCCTGGCAGC CACGCTCCCT GGATATTGAA GTTGCAGCCT ATGCACTGCT CTCACACTTC 
TTACAATTTC AGACTTCTGA GGGAATCCCA ATTATGAGGT GGCTAAGCAG GCAAAGAAAT 
AGCTTGGGTG GTTTTGCATC TACTCAGGAT ACCACTGTGG CTTTAAAGGC TCTGTCTGAA 
TTTGCAGCCC TAATGAATAC AGAAAGGACA AATATCCAAG TGACCGTGAC GGGGCCTAGC 
TCACCAAGTC CTGTAAAGTT TCTGATTGAC ACACACAACC GCTTACTCCT TCAGACAGCA 
GAGCTTGCTG TGGTACAGCC AACGGCAGTT AATATTTCCG CAAATGGTTT TGGATTTGCT 
ATTTGTCAGC TCAATGTTGT ATATAATGTG AAGGCTTCTG GGTCTTCTAG AAGACGAAGA 
TCTATCCAAA ATCAAGAAGC CTTTGATTTA GATGTTGCTG TAAAAGAAAA TAAAGATGAT 
CTCAATCATG TGGATTTGAA TGTGTGTACA AGCTTTTCGG GCCCGGGTAG GAGTGGCATG 
GCTCTTATGG AAGTTAACCT ATTAAGTGGC TTTATGGTGC CTTCAGAAGC AATTTCTCTG 
AGCGAGACAG TGAAGAAAGT GGAATATGAT CATGGAAAAC TCAACCTCTA TTTAGATTCT 
GTAAATGAAA CCCAGTTTTG TGTTAATATT CCTGCTGTGA GAAACTTTAA AGTTTCAAAT 
ACCCAAGATG CTTCAGTGTC CATAGTGGAT TACTATGAGC CAAGGAGACA GGCGGTGAGA 
AGTTACAACT CTGAAGTGAA GCTGTCCTCC TGTGACCTTT GCAGTGATGT CCAGGGCTGC 
CGTCCTTGTG AGGATGGAGC TTCAGGCTCC CATCATCACT CTTCAGTCAT TTTTATTTTC 
TGTTTCAAGC TTCTGTACTT TATGGAACTT TGGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011533775.1
CDS	16..4314
Translation	MWQVCVNNAGSGPRFLVTAPGIIRPGGNVTIGVELLEHCPSQVTVKAELLKTASNLTVSVLEAEGVFEKGSFKTLTLPSLPLNSADEIYELRVTGRTQDEILFSNSTRLSFETKRISVFIQTDKALYKPKQEVKFRIVTLFSDFKPYKTSLNILIKDPKSNLIQQWLSQQSDLGVISKTFQLSSHPILGDWSIQVQVNDQTYYQSFQVSEYVLPKFEVTLQTPLYCSMNSKHLNGTITAKYTYGKPVKGDVTLTFLPLSFWGKKKNITKTFKINGSANFSFNDEEMKNVMDSSNGLSEYLDLSSPGPVEILTTVTESVTGISRNVSTNVFFKQHDYIIEFFDYTTVLKPSLNFTATVKVTRADGNQLTLEERRNNVVITVTQRNYTEYWSGSNSGNQKMEAVQKINYTVPQSGTFKIEFPILEDSSELQLKAYFLGSKSSMAVHSLFKSPSKTYIQLKTRDENIKVGSPFELVVSGNKRLKELSYMVVSRGQLVAVGKQNSTMFSLTPENSWTPKACVIVYYIEDDGEIISDVLKIPVQLVFKNKIKLYWSKVKAEPSEKVSLRISVTQPDSIVGIVAVDKSVNLMNASNDITMENVVHELELYNTGYYLGMFMNSFAVFQECGLWVLTDANLTKDYIDGVYDNAEYAERFMEENEGHIVDIHDFSLGSSPHVRKHFPETWIWLDTNMGYRIYQEFEVTVPDSITSWVATGFVISEDLGLGLTTTPVELQAFQPFFIFLNLPYSVIRGEEFALEITIFNYLKDATEVKVIIEKSDKFDILMTSNEINATGHQQTLLVPSEDGATVLFPIRPTHLGEIPITVTALSPTASDAVTQMILVKAEGIEKSYSQSILLDLTDNRLQSTLKTLSFSFPPNTVTGSERVQITAIGDVLGPSINGLASLIRMPYGCGEQNMINFAPNIYILDYLTKKKQLTDNLKEKALSFMRQGYQRELLYQREDGSFSAFGNYDPSGSTWLSAFVLRCFLEADPYIDIDQNVLHRTYTWLKGHQKSNGEFWDPGRVIHSELQGGNKSPVTLTAYIVTSLLGYRKYQPNIDVQESIHFLESEFSRGISDNYTLALITYALSSVGSPKAKEALNMLTWRAEQEGGMQFWVSSESKLSDSWQPRSLDIEVAAYALLSHFLQFQTSEGIPIMRWLSRQRNSLGGFASTQDTTVALKALSEFAALMNTERTNIQVTVTGPSSPSPVKFLIDTHNRLLLQTAELAVVQPTAVNISANGFGFAICQLNVVYNVKASGSSRRRRSIQNQEAFDLDVAVKENKDDLNHVDLNVCTSFSGPGRSGMALMEVNLLSGFMVPSEAISLSETVKKVEYDHGKLNLYLDSVNETQFCVNIPAVRNFKVSNTQDASVSIVDYYEPRRQAVRSYNSEVKLSSCDLCSDVQGCRPCEDGASGSHHHSSVIFIFCFKLLYFMELWL

Target ORF information:

RefSeq Version	XM_011535473.1
Organism	Homo sapiens(human)
Definition	Homo sapiens CD109 molecule (CD109), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011535473.1

ORF Insert Sequence:

ATGTGGCAAG TGTGTGTGAA CAACGCAGGC TCAGGGCCTC GGTTTCTGGT GACAGCCCCA 
GGGATCATCA GGCCCGGAGG AAATGTGACT ATTGGGGTGG AGCTTCTGGA ACACTGCCCT 
TCACAGGTGA CTGTGAAGGC GGAGCTGCTC AAGACAGCAT CAAACCTCAC TGTCTCTGTC 
CTGGAAGCAG AAGGAGTCTT TGAAAAAGGC TCTTTTAAGA CACTTACTCT TCCATCACTA 
CCTCTGAACA GTGCAGATGA GATTTATGAG CTACGTGTAA CCGGACGTAC CCAGGATGAG 
ATTTTATTCT CTAATAGTAC CCGCTTATCA TTTGAGACCA AGAGAATATC TGTCTTCATT 
CAAACAGACA AGGCCTTATA CAAGCCAAAG CAAGAAGTGA AGTTTCGCAT TGTTACACTC 
TTCTCAGATT TTAAGCCTTA CAAAACCTCT TTAAACATTC TCATTAAGGA CCCCAAATCA 
AATTTGATCC AACAGTGGTT GTCACAACAA AGTGATCTTG GAGTCATTTC CAAAACTTTT 
CAGCTATCTT CCCATCCAAT ACTTGGTGAC TGGTCTATTC AAGTTCAAGT GAATGACCAG 
ACATACTATC AATCATTTCA GGTTTCAGAA TATGTATTAC CAAAATTTGA AGTGACTTTG 
CAGACACCAT TATATTGTTC TATGAATTCT AAGCATTTAA ATGGTACCAT CACGGCAAAG 
TATACATATG GGAAGCCAGT GAAAGGAGAC GTAACGCTTA CATTTTTACC TTTATCCTTT 
TGGGGAAAGA AGAAAAATAT TACAAAAACA TTTAAGATAA ATGGATCTGC AAACTTCTCT 
TTTAATGATG AAGAGATGAA AAATGTAATG GATTCTTCAA ATGGACTTTC TGAATACCTG 
GATCTATCTT CCCCTGGACC AGTAGAAATT TTAACCACAG TGACAGAATC AGTTACAGGT 
ATTTCAAGAA ATGTAAGCAC TAATGTGTTC TTCAAGCAAC ATGATTACAT CATTGAGTTT 
TTTGATTATA CTACTGTCTT GAAGCCATCT CTCAACTTCA CAGCCACTGT GAAGGTAACT 
CGTGCTGATG GCAACCAACT GACTCTTGAA GAAAGAAGAA ATAATGTAGT CATAACAGTG 
ACACAGAGAA ACTATACTGA GTACTGGAGC GGATCTAACA GTGGAAATCA GAAAATGGAA 
GCTGTTCAGA AAATAAATTA TACTGTCCCC CAAAGTGGAA CTTTTAAGAT TGAATTCCCA 
ATCCTGGAGG ATTCCAGTGA GCTACAGTTG AAGGCCTATT TCCTTGGTAG TAAAAGTAGC 
ATGGCAGTTC ATAGTCTGTT TAAGTCTCCT AGTAAGACAT ACATCCAACT AAAAACAAGA 
GATGAAAATA TAAAGGTGGG ATCGCCTTTT GAGTTGGTGG TTAGTGGCAA CAAACGATTG 
AAGGAGTTAA GCTATATGGT AGTATCCAGG GGACAGTTGG TGGCTGTAGG AAAACAAAAT 
TCAACAATGT TCTCTTTAAC ACCAGAAAAT TCTTGGACTC CAAAAGCCTG TGTAATTGTG 
TATTATATTG AAGATGATGG GGAAATTATA AGTGATGTTC TAAAAATTCC TGTTCAGCTT 
GTTTTTAAAA ATAAGATAAA GCTATATTGG AGTAAAGTGA AAGCTGAACC ATCTGAGAAA 
GTCTCTCTTA GGATCTCTGT GACACAGCCT GACTCCATAG TTGGGATTGT AGCTGTTGAC 
AAAAGTGTGA ATCTGATGAA TGCCTCTAAT GATATTACAA TGGAAAATGT GGTCCATGAG 
TTGGAACTTT ATAACACAGG ATATTATTTA GGCATGTTCA TGAATTCTTT TGCAGTCTTT 
CAGGAATGTG GACTCTGGGT ATTGACAGAT GCAAACCTCA CGAAGGATTA TATTGATGGT 
GTTTATGACA ATGCAGAATA TGCTGAGAGG TTTATGGAGG AAAATGAAGG ACATATTGTA 
GATATTCATG ACTTTTCTTT GGGTAGCAGT CCACATGTCC GAAAGCATTT TCCAGAGACT 
TGGATTTGGC TAGACACCAA CATGGGTTAC AGGATTTACC AAGAATTTGA AGTAACTGTA 
CCTGATTCTA TCACTTCTTG GGTGGCTACT GGTTTTGTGA TCTCTGAGGA CCTGGGTCTT 
GGACTAACAA CTACTCCAGT GGAGCTCCAA GCCTTCCAAC CATTTTTCAT TTTTTTGAAT 
CTTCCCTACT CTGTTATCAG AGGTGAAGAA TTTGCTTTGG AAATAACTAT ATTCAATTAT 
TTGAAAGATG CCACTGAGGT TAAGGTAATC ATTGAGAAAA GTGACAAATT TGATATTCTA 
ATGACTTCAA ATGAAATAAA TGCCACAGGC CACCAGCAGA CCCTTCTGGT TCCCAGTGAG 
GATGGGGCAA CTGTTCTTTT TCCCATCAGG CCAACACATC TGGGAGAAAT TCCTATCACA 
GTCACAGCTC TTTCACCCAC TGCTTCTGAT GCTGTCACCC AGATGATTTT AGTAAAGGCT 
GAAGGAATAG AAAAATCATA TTCACAATCC ATCTTATTAG ACTTGACTGA CAATAGGCTA 
CAGAGTACCC TGAAAACTTT GAGTTTCTCA TTTCCTCCTA ATACAGTGAC TGGCAGTGAA 
AGAGTTCAGA TCACTGCAAT TGGAGATGTT CTTGGTCCTT CCATCAATGG CTTAGCCTCA 
TTGATTCGGA TGCCTTATGG CTGTGGTGAA CAGAACATGA TAAATTTTGC TCCAAATATT 
TACATTTTGG ATTATCTGAC TAAAAAGAAA CAACTGACAG ATAATTTGAA AGAAAAAGCT 
CTTTCATTTA TGAGGCAAGG TTACCAGAGA GAACTTCTCT ATCAGAGGGA AGATGGCTCT 
TTCAGTGCTT TTGGGAATTA TGACCCTTCT GGGAGCACTT GGTTGTCAGC TTTTGTTTTA 
AGATGTTTCC TTGAAGCCGA TCCTTACATA GATATTGATC AGAATGTGTT ACACAGAACA 
TACACTTGGC TTAAAGGACA TCAGAAATCC AACGGTGAAT TTTGGGATCC AGGAAGAGTG 
ATTCATAGTG AGCTTCAAGG TGGCAATAAA AGTCCAGTAA CACTTACAGC CTATATTGTA 
ACTTCTCTCC TGGGATATAG AAAGTATCAG CCTAACATTG ATGTGCAAGA GTCTATCCAT 
TTTTTGGAGT CTGAATTCAG TAGAGGAATT TCAGACAATT ATACTCTAGC CCTTATAACT 
TATGCATTGT CATCAGTGGG GAGTCCTAAA GCGAAGGAAG CTTTGAATAT GCTGACTTGG 
AGAGCAGAAC AAGAAGGTGG CATGCAATTC TGGGTGTCAT CAGAGTCCAA ACTTTCTGAC 
TCCTGGCAGC CACGCTCCCT GGATATTGAA GTTGCAGCCT ATGCACTGCT CTCACACTTC 
TTACAATTTC AGACTTCTGA GGGAATCCCA ATTATGAGGT GGCTAAGCAG GCAAAGAAAT 
AGCTTGGGTG GTTTTGCATC TACTCAGGAT ACCACTGTGG CTTTAAAGGC TCTGTCTGAA 
TTTGCAGCCC TAATGAATAC AGAAAGGACA AATATCCAAG TGACCGTGAC GGGGCCTAGC 
TCACCAAGTC CTGTAAAGTT TCTGATTGAC ACACACAACC GCTTACTCCT TCAGACAGCA 
GAGCTTGCTG TGGTACAGCC AACGGCAGTT AATATTTCCG CAAATGGTTT TGGATTTGCT 
ATTTGTCAGC TCAATGTTGT ATATAATGTG AAGGCTTCTG GGTCTTCTAG AAGACGAAGA 
TCTATCCAAA ATCAAGAAGC CTTTGATTTA GATGTTGCTG TAAAAGAAAA TAAAGATGAT 
CTCAATCATG TGGATTTGAA TGTGTGTACA AGCTTTTCGG GCCCGGGTAG GAGTGGCATG 
GCTCTTATGG AAGTTAACCT ATTAAGTGGC TTTATGGTGC CTTCAGAAGC AATTTCTCTG 
AGCGAGACAG TGAAGAAAGT GGAATATGAT CATGGAAAAC TCAACCTCTA TTTAGATTCT 
GTAAATGAAA CCCAGTTTTG TGTTAATATT CCTGCTGTGA GAAACTTTAA AGTTTCAAAT 
ACCCAAGATG CTTCAGTGTC CATAGTGGAT TACTATGAGC CAAGGAGACA GGCGGTGAGA 
AGTTACAACT CTGAAGTGAA GCTGTCCTCC TGTGACCTTT GCAGTGATGT CCAGGGCTGC 
CGTCCTTGTG AGGATGGAGC TTCAGGCTCC CATCATCACT CTTCAGTCAT TTTTATTTTC 
TGTTTCAAGC TTCTGTACTT TATGGAACTT TGGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Significance of perivascular tumour cells defined by CD109 expression in progression of glioma.
The Journal of pathology243(4)468-480(2017 Dec)
Shiraki Y,Mii S,Enomoto A,Momota H,Han YP,Kato T,Ushida K,Kato A,Asai N,Murakumo Y,Aoki K,Suzuki H,Ohka F,Wakabayashi T,Todo T,Ogawa S,Natsume A,Takahashi M

CD109, a negative regulator of TGF-? signaling, is a putative risk marker in diffuse large B-cell lymphoma.
International journal of hematology105(5)614-622(2017 May)
Yokoyama M,Ichinoe M,Okina S,Sakurai Y,Nakada N,Yanagisawa N,Jiang SX,Numata Y,Umezawa A,Miyazaki K,Higashihara M,Murakumo Y

CD109 Mediates Cell Survival in Hepatocellular Carcinoma Cells.
Digestive diseases and sciences61(8)2303-14(2016 08)
Zong G,Xu Z,Zhang S,Shen Y,Qiu H,Zhu G,He S,Tao T,Chen X

Reduced expression of CD109 in tumor-associated endothelial cells promotes tumor progression by paracrine interleukin-8 in hepatocellular carcinoma.
Oncotarget7(20)29333-45(2016 May)
Ye BG,Sun HC,Zhu XD,Chai ZT,Zhang YY,Ao JY,Cai H,Ma DN,Wang CH,Qin CD,Gao DM,Tang ZY

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CD109 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム