HSPB6 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HSPB6
Entrez Gene ID	126393
Full Name	heat shock protein family B (small) member 6
Synonyms	HEL55,Hsp20,PPP1R91
General protein information	Preferred Names heat shock protein family B (small) member 6 Names heat shock protein beta-6 epididymis luminal protein 55 heat shock 20 kDa-like protein p20 heat shock protein family B (small) member B6 heat shock protein, alpha-crystallin-related, B6 protein phosphatase 1, regulatory subunit 91
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.12
Summary	This locus encodes a heat shock protein. The encoded protein likely plays a role in smooth muscle relaxation. [provided by RefSeq, Jan 2012].
Disorder MIM:	610695

mRNA and Protein(s)

mRNA	Protein	Name
NM_144617.2	NP_653218.1	heat shock protein beta-6
NM_144617.3	NP_653218.1	heat shock protein beta-6

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	HSPB6	XP_003316346.1
Canis lupus familiaris (dog)	HSPB6	XP_541688.1
Bos taurus (cattle)	HSPB6	NP_001069495.1
Homo sapiens (human)	HSPB6	NP_653218.1
	HSPB6	XP_001098599.1
Mus musculus (house mouse)	Hspb6	NP_001012401.1
Rattus norvegicus (Norway rat)	Hspb6	NP_620242.1

Gene Ontology
Bibliography

Related articles in PubMed

Regulation of BECN1-Mediated Autophagy by HSPB6: Insights from A Human HSPB6
Liu GS, Zhu H, Cai WF, Wang X, Jiang M, Essandoh K, Vafiadaki E, Haghighi K, Lam CK, Gardner G, Adly G, Nicolaou P, Sanoudou D, Liang Q, Rubinstein J, Fan GC, Kranias EG
Autophagy1-56(2017 Nov)

Specific sequences in the N-terminal domain of human small heat-shock protein HSPB6 dictate preferential hetero-oligomerization with the orthologue HSPB1.
Heirbaut M, Lermyte F, Martin EM, Beelen S, Sobott F, Strelkov SV, Weeks SD
The Journal of biological chemistry292(24)9944-9957(2017 06)

Structural Basis for the Interaction of a Human Small Heat Shock Protein with the 14-3-3 Universal Signaling Regulator.
Sluchanko NN, Beelen S, Kulikova AA, Weeks SD, Antson AA, Gusev NB, Strelkov SV
Structure (London, England : 1993)25(2)305-316(2017 02)

Phosphorylated Heat Shock Protein 20 (HSPB6) Regulates Transforming Growth Factor-?-Induced Migration and Invasion of Hepatocellular Carcinoma Cells.
Matsushima-Nishiwaki R, Toyoda H, Nagasawa T, Yasuda E, Chiba N, Okuda S, Maeda A, Kaneoka Y, Kumada T, Kozawa O
PloS one11(4)e0151907(2016)

Small heat shock proteins are induced during multiple sclerosis lesion development in white but not grey matter.
Peferoen LA, Gerritsen WH, Breur M, Ummenthum KM, Peferoen-Baert RM, van der Valk P, van Noort JM, Amor S
Acta neuropathologica communications387(2015 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that HSPB6 forms hetero-oligomers with HSPB1 under the following rules: (1) highly conserved motif RLFDQXFG is necessary for subunit exchange among oligomers, (2) a site about 20 residues downstream of this motif determines size of resultant hetero-oligomers, and (3) a region in the N-terminal domain that is unique to HSPB6 dictates preferential formation of heterodimers. (HSP = heat shock protein)
Title: Specific sequences in the N-terminal domain of human small heat-shock protein HSPB6 dictate preferential hetero-oligomerization with the orthologue HSPB1.

Structural basis for the interaction of a human HSPB6 protein with the 14-3-3 universal signaling regulator has been reported.
Title: Structural Basis for the Interaction of a Human Small Heat Shock Protein with the 14-3-3 Universal Signaling Regulator.

findings strongly suggest that phosphorylated HSP20 inhibits TGF-alpha-induced HCC cell migration and invasion via suppression of the JNK signaling pathway
Title: Phosphorylated Heat Shock Protein 20 (HSPB6) Regulates Transforming Growth Factor-?-Induced Migration and Invasion of Hepatocellular Carcinoma Cells.

multiple sclerosis lesions revealed exclusive induction of HSPB6 in astrocytes, as confirmed by co-localization of HSPB6 with GFAP.
Title: Small heat shock proteins are induced during multiple sclerosis lesion development in white but not grey matter.

N-terminal mutations increase stability of large HspB1 homooligomers, prevent their phosphorylation-dependent dissociation, modulate their interaction with HspB6 and decrease their chaperoning capacity, preventing normal functioning of HspB1.
Title: Characterization of Mutants of Human Small Heat Shock Protein HspB1 Carrying Replacements in the N-Terminal Domain and Associated with Hereditary Motor Neuron Diseases.

The following HSPB6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HSPB6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu28040	NM_144617.3 Latest version!	Homo sapiens heat shock protein family B (small) member 6 (HSPB6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu28040	NM_144617.2	Homo sapiens heat shock protein family B (small) member 6 (HSPB6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HSPB6 ( NM_144617.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_653218.1 Homo sapiens heat shock protein family B (small) member 6 (HSPB6), mRNA.

Price & Availability↑

CloneID	OHu28040
Clone ID Related Accession (Same CDS sequence)	NM_144617.2 , NM_144617.3
Accession Version	NM_144617.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-08
Organism	Homo sapiens(human)
Product	heat shock protein beta-6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK056951.1, BC068046.1 and AK026458.1. This sequence is a reference standard in the RefSeqGene project. On Jan 4, 2012 this sequence version replaced NM_144617.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK056951.1, BC068046.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END##

ATGGAGATCC CTGTGCCTGT GCAGCCGTCT TGGCTGCGCC GCGCCTCGGC CCCGTTGCCC 
GGACTTTCGG CGCCCGGACG CCTCTTTGAC CAGCGCTTCG GCGAGGGGCT GCTGGAGGCC 
GAGCTGGCTG CGCTCTGCCC CACCACGCTC GCCCCCTACT ACCTGCGCGC ACCCAGCGTG 
GCGCTGCCCG TCGCCCAGGT GCCGACGGAC CCCGGCCACT TTTCGGTGCT GCTAGACGTG 
AAGCACTTCT CGCCGGAGGA AATTGCTGTC AAGGTGGTGG GCGAACACGT GGAGGTGCAC 
GCGCGCCACG AGGAGCGCCC GGATGAGCAC GGATTCGTCG CGCGCGAGTT CCACCGTCGC 
TACCGCCTGC CGCCTGGCGT GGATCCGGCT GCCGTGACGT CCGCGCTGTC CCCCGAGGGC 
GTCCTGTCCA TCCAGGCCGC ACCAGCGTCG GCCCAGGCCC CACCGCCAGC CGCAGCCAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_653218.1
CDS	22..504
Translation	MEIPVPVQPSWLRRASAPLPGLSAPGRLFDQRFGEGLLEAELAALCPTTLAPYYLRAPSVALPVAQVPTDPGHFSVLLDVKHFSPEEIAVKVVGEHVEVHARHEERPDEHGFVAREFHRRYRLPPGVDPAAVTSALSPEGVLSIQAAPASAQAPPPAAAK

Target ORF information:

RefSeq Version	NM_144617.2
Organism	Homo sapiens(human)
Definition	Homo sapiens heat shock protein family B (small) member 6 (HSPB6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144617.2

ORF Insert Sequence:

ATGGAGATCC CTGTGCCTGT GCAGCCGTCT TGGCTGCGCC GCGCCTCGGC CCCGTTGCCC 
GGACTTTCGG CGCCCGGACG CCTCTTTGAC CAGCGCTTCG GCGAGGGGCT GCTGGAGGCC 
GAGCTGGCTG CGCTCTGCCC CACCACGCTC GCCCCCTACT ACCTGCGCGC ACCCAGCGTG 
GCGCTGCCCG TCGCCCAGGT GCCGACGGAC CCCGGCCACT TTTCGGTGCT GCTAGACGTG 
AAGCACTTCT CGCCGGAGGA AATTGCTGTC AAGGTGGTGG GCGAACACGT GGAGGTGCAC 
GCGCGCCACG AGGAGCGCCC GGATGAGCAC GGATTCGTCG CGCGCGAGTT CCACCGTCGC 
TACCGCCTGC CGCCTGGCGT GGATCCGGCT GCCGTGACGT CCGCGCTGTC CCCCGAGGGC 
GTCCTGTCCA TCCAGGCCGC ACCAGCGTCG GCCCAGGCCC CACCGCCAGC CGCAGCCAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HSPB6 ( NM_144617.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_653218.1 Homo sapiens heat shock protein family B (small) member 6 (HSPB6), mRNA.

Price & Availability↑

CloneID	OHu28040
Clone ID Related Accession (Same CDS sequence)	NM_144617.2 , NM_144617.3
Accession Version	NM_144617.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	heat shock protein beta-6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK056951.1, BC068046.1 and AK026458.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_144617.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK056951.1, SRR3476690.1067911.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000004982.6/ ENSP00000004982.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGATCC CTGTGCCTGT GCAGCCGTCT TGGCTGCGCC GCGCCTCGGC CCCGTTGCCC 
GGACTTTCGG CGCCCGGACG CCTCTTTGAC CAGCGCTTCG GCGAGGGGCT GCTGGAGGCC 
GAGCTGGCTG CGCTCTGCCC CACCACGCTC GCCCCCTACT ACCTGCGCGC ACCCAGCGTG 
GCGCTGCCCG TCGCCCAGGT GCCGACGGAC CCCGGCCACT TTTCGGTGCT GCTAGACGTG 
AAGCACTTCT CGCCGGAGGA AATTGCTGTC AAGGTGGTGG GCGAACACGT GGAGGTGCAC 
GCGCGCCACG AGGAGCGCCC GGATGAGCAC GGATTCGTCG CGCGCGAGTT CCACCGTCGC 
TACCGCCTGC CGCCTGGCGT GGATCCGGCT GCCGTGACGT CCGCGCTGTC CCCCGAGGGC 
GTCCTGTCCA TCCAGGCCGC ACCAGCGTCG GCCCAGGCCC CACCGCCAGC CGCAGCCAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_653218.1
CDS	22..504
Translation	MEIPVPVQPSWLRRASAPLPGLSAPGRLFDQRFGEGLLEAELAALCPTTLAPYYLRAPSVALPVAQVPTDPGHFSVLLDVKHFSPEEIAVKVVGEHVEVHARHEERPDEHGFVAREFHRRYRLPPGVDPAAVTSALSPEGVLSIQAAPASAQAPPPAAAK

Target ORF information:

RefSeq Version	NM_144617.3
Organism	Homo sapiens(human)
Definition	Homo sapiens heat shock protein family B (small) member 6 (HSPB6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144617.3

ORF Insert Sequence:

ATGGAGATCC CTGTGCCTGT GCAGCCGTCT TGGCTGCGCC GCGCCTCGGC CCCGTTGCCC 
GGACTTTCGG CGCCCGGACG CCTCTTTGAC CAGCGCTTCG GCGAGGGGCT GCTGGAGGCC 
GAGCTGGCTG CGCTCTGCCC CACCACGCTC GCCCCCTACT ACCTGCGCGC ACCCAGCGTG 
GCGCTGCCCG TCGCCCAGGT GCCGACGGAC CCCGGCCACT TTTCGGTGCT GCTAGACGTG 
AAGCACTTCT CGCCGGAGGA AATTGCTGTC AAGGTGGTGG GCGAACACGT GGAGGTGCAC 
GCGCGCCACG AGGAGCGCCC GGATGAGCAC GGATTCGTCG CGCGCGAGTT CCACCGTCGC 
TACCGCCTGC CGCCTGGCGT GGATCCGGCT GCCGTGACGT CCGCGCTGTC CCCCGAGGGC 
GTCCTGTCCA TCCAGGCCGC ACCAGCGTCG GCCCAGGCCC CACCGCCAGC CGCAGCCAAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Regulation of BECN1-Mediated Autophagy by HSPB6: Insights from A Human HSPB6
Autophagy1-56(2017 Nov)
Liu GS,Zhu H,Cai WF,Wang X,Jiang M,Essandoh K,Vafiadaki E,Haghighi K,Lam CK,Gardner G,Adly G,Nicolaou P,Sanoudou D,Liang Q,Rubinstein J,Fan GC,Kranias EG

Specific sequences in the N-terminal domain of human small heat-shock protein HSPB6 dictate preferential hetero-oligomerization with the orthologue HSPB1.
The Journal of biological chemistry292(24)9944-9957(2017 06)
Heirbaut M,Lermyte F,Martin EM,Beelen S,Sobott F,Strelkov SV,Weeks SD

Structural Basis for the Interaction of a Human Small Heat Shock Protein with the 14-3-3 Universal Signaling Regulator.
Structure (London, England : 1993)25(2)305-316(2017 02)
Sluchanko NN,Beelen S,Kulikova AA,Weeks SD,Antson AA,Gusev NB,Strelkov SV

Phosphorylated Heat Shock Protein 20 (HSPB6) Regulates Transforming Growth Factor-?-Induced Migration and Invasion of Hepatocellular Carcinoma Cells.
PloS one11(4)e0151907(2016)
Matsushima-Nishiwaki R,Toyoda H,Nagasawa T,Yasuda E,Chiba N,Okuda S,Maeda A,Kaneoka Y,Kumada T,Kozawa O

Small heat shock proteins are induced during multiple sclerosis lesion development in white but not grey matter.
Acta neuropathologica communications387(2015 Dec)
Peferoen LA,Gerritsen WH,Breur M,Ummenthum KM,Peferoen-Baert RM,van der Valk P,van Noort JM,Amor S