MBD3L2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MBD3L2
Entrez Gene ID	125997
Full Name	methyl-CpG binding domain protein 3 like 2
General protein information	Preferred Names methyl-CpG binding domain protein 3 like 2 Names methyl-CpG-binding domain protein 3-like 2 CTB-25J19.1 MBD3-like protein 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.2
Summary	This gene encodes a protein that is related to methyl-CpG-binding proteins but lacks the methyl-CpG binding domain. The protein has been found in germ cell tumors and some somatic tissues. [provided by RefSeq, Jul 2008].
Disorder MIM:	607964

mRNA and Protein(s)

mRNA	Protein	Name
NM_144614.3	NP_653215.2	methyl-CpG-binding domain protein 3-like 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	MBD3L4	NP_001157891.1
Homo sapiens (human)	MBD3L3	NP_001157897.1
Pan troglodytes (chimpanzee)	MBD3L2	XP_001165954.1
Rattus norvegicus (Norway rat)	LOC100363016	XP_002729925.1
Rattus norvegicus (Norway rat)	LOC100912114	XP_003752238.1
Pan troglodytes (chimpanzee)	MBD3L2	XP_001144438.1
Pan troglodytes (chimpanzee)	MBD3L2	XP_524079.2
	LOC705273	XP_001093645.1
	LOC705142	XP_001093522.1
Bos taurus (cattle)	MBD3L3	XP_002688877.2
Mus musculus (house mouse)	Mbd3l2	NP_659183.3
Rattus norvegicus (Norway rat)	Mbd3l2	XP_235852.3
Canis lupus familiaris (dog)	LOC100688619	XP_003432915.2
Homo sapiens (human)	LOC729458	XP_001130308.1
Pan troglodytes (chimpanzee)	LOC749125	XP_001165749.2
Pan troglodytes (chimpanzee)	MBD3L2	XP_001165919.1
Pan troglodytes (chimpanzee)	MBD3L2	XP_001165993.1
Homo sapiens (human)	MBD3L5	NP_001129979.1
Homo sapiens (human)	MBD3L2	NP_653215.2

Gene Ontology
Bibliography

Related articles in PubMed

MBD3L2 promotes Tet2 enzymatic activity for mediating 5-methylcytosine oxidation.
Peng L, Li Y, Xi Y, Li W, Li J, Lv R, Zhang L, Zou Q, Dong S, Luo H, Wu F, Yu W
Journal of cell science129(5)1059-71(2016 Mar)

Unique features of the anti-parallel, heterodimeric coiled-coil interaction between methyl-cytosine binding domain 2 (MBD2) homologues and GATA zinc finger domain containing 2A (GATAD2A/p66?).
Walavalkar NM, Gordon N, Williams DC Jr
The Journal of biological chemistry288(5)3419-27(2013 Feb)

MBD3L2 interacts with MBD3 and components of the NuRD complex and can oppose MBD2-MeCP1-mediated methylation silencing.
Jin SG, Jiang CL, Rauch T, Li H, Pfeifer GP
The Journal of biological chemistry280(13)12700-9(2005 Apr)

MBD3L1 and MBD3L2, two new proteins homologous to the methyl-CpG-binding proteins MBD2 and MBD3: characterization of MBD3L1 as a testis-specific transcriptional repressor.
Jiang CL, Jin SG, Lee DH, Lan ZJ, Xu X, O'Connor TR, Szab? PE, Mann JR, Cooney AJ, Pfeifer GP
Genomics80(6)621-9(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

MBD3L2 is more effective than MBD3 in promoting Tet2 enzymatic activity through strengthening the binding affinity between Tet2 and the methylated DNA target.
Title: MBD3L2 promotes Tet2 enzymatic activity for mediating 5-methylcytosine oxidation.

These factors lead to a binding affinity hierarchy of p66alpha for the different MBD2 homologues (MBD2 approximately MBD3 > MBD3L1 approximately MBD3L2).
Title: Unique features of the anti-parallel, heterodimeric coiled-coil interaction between methyl-cytosine binding domain 2 (MBD2) homologues and GATA zinc finger domain containing 2A (GATAD2A/p66?).

MBD3L2 interacts with MBD3 and components of the NuRD complex and can oppose MBD2-MeCP1-mediated methylation silencing
Title: MBD3L2 interacts with MBD3 and components of the NuRD complex and can oppose MBD2-MeCP1-mediated methylation silencing.

The following MBD3L2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MBD3L2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu08890	NM_144614.3 Latest version!	Homo sapiens methyl-CpG binding domain protein 3 like 2 (MBD3L2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$134.50-$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MBD3L2 ( NM_144614.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_653215.2 Homo sapiens methyl-CpG binding domain protein 3 like 2 (MBD3L2), mRNA.

Price & Availability↑

CloneID	OHu08890
Clone ID Related Accession (Same CDS sequence)	NM_144614.3
Accession Version	NM_144614.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Homo sapiens(human)
Product	methyl-CpG-binding domain protein 3-like 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010606.7 and AF503919.2. On Sep 20, 2007 this sequence version replaced NM_144614.2. ##Evidence-Data-START## Transcript exon combination :: AF503919.2, BE281376.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMN03465418 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGAGAAC CTGCGTTCAC CTCTTTTCCA AGCCTGCCTG TTCTGGGGAA GCTCAAAAGG 
AACATGATGC CCTGGGCTTT ACAGAAGAAA CGAGAAATCC ACATGGCCAA GGCCCATCGG 
AGACGAGCTG CGAGGTCTGC TCTCCCCATG AGACTCACCA GCTGCATCTT CCGGAGGCCG 
GTGACAAGGA TCAGGTCTCA TCCTGACAAC CAGGTCAGAC GCAGAAAAGG GGACGAGCAC 
CTGGAGAAGC CGCAGCAACT CTGCGCCTAC CGGAGACTGC AGGCCCTGCA GCCCTGCAGC 
AGCCAAGGAG AAGGTTCAAG TCCACTGCAT TTGGAGAGCG TCTTAAGTAT CCTTGCACCG 
GGGACGGCCG GTGAATCTCT GGACAGAGCT GGTGCTGAGC GTGTGCGCAG CCCGCTTGAG 
CCCACCCCTG GGCGGTTTCC AGCTGTGGCA GGGGGGCCAA CCCCAGGAAT GGGTTGTCAG 
CTCCCACCGC CCCTCTCTGG CCAATTGGTG ACTCCTGCAG ATATCCGGAG ACAGGCCAGG 
AGGGTGAAGA AAGCCAGGGA GAGACTGGCC AAGGCCTTGC AGGCAGACAG GCTGGCCAGG 
CGGGCAGAAA TGCTGACAGG TGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_653215.2
CDS	35..661
Translation	MGEPAFTSFPSLPVLGKLKRNMMPWALQKKREIHMAKAHRRRAARSALPMRLTSCIFRRPVTRIRSHPDNQVRRRKGDEHLEKPQQLCAYRRLQALQPCSSQGEGSSPLHLESVLSILAPGTAGESLDRAGAERVRSPLEPTPGRFPAVAGGPTPGMGCQLPPPLSGQLVTPADIRRQARRVKKARERLAKALQADRLARRAEMLTGG

Target ORF information:

RefSeq Version	NM_144614.3
Organism	Homo sapiens(human)
Definition	Homo sapiens methyl-CpG binding domain protein 3 like 2 (MBD3L2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144614.3

ORF Insert Sequence:

ATGGGAGAAC CTGCGTTCAC CTCTTTTCCA AGCCTGCCTG TTCTGGGGAA GCTCAAAAGG 
AACATGATGC CCTGGGCTTT ACAGAAGAAA CGAGAAATCC ACATGGCCAA GGCCCATCGG 
AGACGAGCTG CGAGGTCTGC TCTCCCCATG AGACTCACCA GCTGCATCTT CCGGAGGCCG 
GTGACAAGGA TCAGGTCTCA TCCTGACAAC CAGGTCAGAC GCAGAAAAGG GGACGAGCAC 
CTGGAGAAGC CGCAGCAACT CTGCGCCTAC CGGAGACTGC AGGCCCTGCA GCCCTGCAGC 
AGCCAAGGAG AAGGTTCAAG TCCACTGCAT TTGGAGAGCG TCTTAAGTAT CCTTGCACCG 
GGGACGGCCG GTGAATCTCT GGACAGAGCT GGTGCTGAGC GTGTGCGCAG CCCGCTTGAG 
CCCACCCCTG GGCGGTTTCC AGCTGTGGCA GGGGGGCCAA CCCCAGGAAT GGGTTGTCAG 
CTCCCACCGC CCCTCTCTGG CCAATTGGTG ACTCCTGCAG ATATCCGGAG ACAGGCCAGG 
AGGGTGAAGA AAGCCAGGGA GAGACTGGCC AAGGCCTTGC AGGCAGACAG GCTGGCCAGG 
CGGGCAGAAA TGCTGACAGG TGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MBD3L2 promotes Tet2 enzymatic activity for mediating 5-methylcytosine oxidation.
Journal of cell science129(5)1059-71(2016 Mar)
Peng L,Li Y,Xi Y,Li W,Li J,Lv R,Zhang L,Zou Q,Dong S,Luo H,Wu F,Yu W

Unique features of the anti-parallel, heterodimeric coiled-coil interaction between methyl-cytosine binding domain 2 (MBD2) homologues and GATA zinc finger domain containing 2A (GATAD2A/p66?).
The Journal of biological chemistry288(5)3419-27(2013 Feb)
Walavalkar NM,Gordon N,Williams DC Jr

MBD3L2 interacts with MBD3 and components of the NuRD complex and can oppose MBD2-MeCP1-mediated methylation silencing.
The Journal of biological chemistry280(13)12700-9(2005 Apr)
Jin SG,Jiang CL,Rauch T,Li H,Pfeifer GP

MBD3L1 and MBD3L2, two new proteins homologous to the methyl-CpG-binding proteins MBD2 and MBD3: characterization of MBD3L1 as a testis-specific transcriptional repressor.
Genomics80(6)621-9(2002 Dec)
Jiang CL,Jin SG,Lee DH,Lan ZJ,Xu X,O'Connor TR,Szab? PE,Mann JR,Cooney AJ,Pfeifer GP